TY  - JOUR
AU  - Stanković, Milica
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
AU  - Arsić, Ivana A.
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3811
AB  - Oils from wild fruits have gained attention due to the presence of the wide variety of natural bioactive lipid components (essential polyunsaturated fatty acids, phytosterols, lipid-soluble vitamins), which have positive effects on human health. The present study describes the extraction of oils from fruits of different plant species, Sambucus nigra, Prunus spinosa, Sorbus aucuparia and Rosa canina, using the conventional Soxhlet extraction technique with petroleum ether. Before the extraction, mechanical processing of fruit samples (exposure to elevated pressure and temperature) was performed. The yield of oil (%) was expressed as mass of obtained oil per mass of dry plant material (w/w). Moisture content of fruit samples was also determined. Wild fruits showed different moisture contents, ranging from 6.53 to 71.52 g/100 g of fresh weight, in R. canina and S. aucuparia fruits, respectively. S. nigra and P. spinosa fruits revealed similar moisture contents, 68.48 g/100 g and 68.89 g/100 g of fresh weight, respectively. Oil yields of the investigated fruit samples showed statistically significant differences (p  lt  0.05) and ranged from 0.38 to 3.22% (w/w) in the R. canina and P. spinosa fruits, respectively. Dry fruits of S. nigra had 2.55% (w/w) of oil, while the oil content in S. aucuparia fruits was 0.68% (w/w). The findings obtained from this study might be useful from the aspect of potential utilization of these wild fruits, in particular fruits of P. spinosa and S. nigra, as raw materials for the extraction of valuable oil for the application in food, cosmetic and pharmaceutical industry.
AB  - Ulja dobijena iz plodova biljaka sve više dobijaju na značaju, upravo zbog prisustva velikog broja prirodnih, bioaktivnih lipidnih komponenata (esencijalnih, polinezasićenih masnih kiselina, fitosterola, liposolubilnih vitamina), koji ispoljavaju pozitivne efekte na ljudsko zdravlje. U ovom radu opisan je postupak ekstrakcije ulja iz plodova različitih biljnih vrsta (Sambucus nigra, Prunus spinosa, Sorbus aucuparia i Rosa canina), primenom konvencionalne Soxhlet ekstrakcione tehnike sa petrol-etrom. Pre ekstrakcije izvršena je mehanička obrada uzoraka plodova, njihovim izlaganjem povišenom pritisku i temperaturi. Prinos ulja (%) je izražen kao masa dobijenog ulja u odnosu na masu suvog biljnog materijala (w/w). Određen je i sadržaj vlage u uzorcima plodova. Divlji plodovi su se razlikovali po sadržaju vlage, čije su vrednosti bile u granicama od 6,53 g/100 g svežih plodova (R. canina) do 71,52 g/100 g svežih plodova (S. aucuparia). Biljne vrste S. nigra i P. spinosa pokazale su sličan sadržaj vlage u plodovima, i to 68,48 g/100 g i 68,89 g/100 g svežih plodova. Prinosi ulja ispitivanih uzoraka plodova razlikovali su se statistički značajno (p  lt  0,05), a vrednosti su bile od 0,38% (kod R. canina plodova) do 3,22% (plodovi P. spinosa). Kod suvih plodova S. nigra dobijeni prinos ulja je iznosio 2,55%, dok je sadržaj ulja u plodovima S. aucuparia iznosio 0,68%. Dobijeni rezultati su korisni zbog mogućnosti upotrebe ispitivanih divljih plodova, naročito plodova P. spinosa i S. nigra (imale su najveći sadržaj ulja), kao polaznih sirovina za ekstrakciju biljnog ulja za primenu u prehrambenoj, kozmetičkoj i farmaceutskoj industriji.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique
T1  - Sadržaj ulja divljih plodova različitih biljnih vrsta dobijen konvencionalnom Soxhlet tehnikom ekstrakcije
EP  - 200
IS  - 3
SP  - 193
VL  - 35
DO  - 10.2478/afmnai-2018-0021
ER  - 

@article{
author = "Stanković, Milica and Maksimović, Svetolik and Tadić, Vanja and Arsić, Ivana A.",
year = "2018",
abstract = "Oils from wild fruits have gained attention due to the presence of the wide variety of natural bioactive lipid components (essential polyunsaturated fatty acids, phytosterols, lipid-soluble vitamins), which have positive effects on human health. The present study describes the extraction of oils from fruits of different plant species, Sambucus nigra, Prunus spinosa, Sorbus aucuparia and Rosa canina, using the conventional Soxhlet extraction technique with petroleum ether. Before the extraction, mechanical processing of fruit samples (exposure to elevated pressure and temperature) was performed. The yield of oil (%) was expressed as mass of obtained oil per mass of dry plant material (w/w). Moisture content of fruit samples was also determined. Wild fruits showed different moisture contents, ranging from 6.53 to 71.52 g/100 g of fresh weight, in R. canina and S. aucuparia fruits, respectively. S. nigra and P. spinosa fruits revealed similar moisture contents, 68.48 g/100 g and 68.89 g/100 g of fresh weight, respectively. Oil yields of the investigated fruit samples showed statistically significant differences (p  lt  0.05) and ranged from 0.38 to 3.22% (w/w) in the R. canina and P. spinosa fruits, respectively. Dry fruits of S. nigra had 2.55% (w/w) of oil, while the oil content in S. aucuparia fruits was 0.68% (w/w). The findings obtained from this study might be useful from the aspect of potential utilization of these wild fruits, in particular fruits of P. spinosa and S. nigra, as raw materials for the extraction of valuable oil for the application in food, cosmetic and pharmaceutical industry., Ulja dobijena iz plodova biljaka sve više dobijaju na značaju, upravo zbog prisustva velikog broja prirodnih, bioaktivnih lipidnih komponenata (esencijalnih, polinezasićenih masnih kiselina, fitosterola, liposolubilnih vitamina), koji ispoljavaju pozitivne efekte na ljudsko zdravlje. U ovom radu opisan je postupak ekstrakcije ulja iz plodova različitih biljnih vrsta (Sambucus nigra, Prunus spinosa, Sorbus aucuparia i Rosa canina), primenom konvencionalne Soxhlet ekstrakcione tehnike sa petrol-etrom. Pre ekstrakcije izvršena je mehanička obrada uzoraka plodova, njihovim izlaganjem povišenom pritisku i temperaturi. Prinos ulja (%) je izražen kao masa dobijenog ulja u odnosu na masu suvog biljnog materijala (w/w). Određen je i sadržaj vlage u uzorcima plodova. Divlji plodovi su se razlikovali po sadržaju vlage, čije su vrednosti bile u granicama od 6,53 g/100 g svežih plodova (R. canina) do 71,52 g/100 g svežih plodova (S. aucuparia). Biljne vrste S. nigra i P. spinosa pokazale su sličan sadržaj vlage u plodovima, i to 68,48 g/100 g i 68,89 g/100 g svežih plodova. Prinosi ulja ispitivanih uzoraka plodova razlikovali su se statistički značajno (p  lt  0,05), a vrednosti su bile od 0,38% (kod R. canina plodova) do 3,22% (plodovi P. spinosa). Kod suvih plodova S. nigra dobijeni prinos ulja je iznosio 2,55%, dok je sadržaj ulja u plodovima S. aucuparia iznosio 0,68%. Dobijeni rezultati su korisni zbog mogućnosti upotrebe ispitivanih divljih plodova, naročito plodova P. spinosa i S. nigra (imale su najveći sadržaj ulja), kao polaznih sirovina za ekstrakciju biljnog ulja za primenu u prehrambenoj, kozmetičkoj i farmaceutskoj industriji.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique, Sadržaj ulja divljih plodova različitih biljnih vrsta dobijen konvencionalnom Soxhlet tehnikom ekstrakcije",
pages = "200-193",
number = "3",
volume = "35",
doi = "10.2478/afmnai-2018-0021"
}

Stanković, M., Maksimović, S., Tadić, V.,& Arsić, I. A.. (2018). The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique. in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 35(3), 193-200.
https://doi.org/10.2478/afmnai-2018-0021

Stanković M, Maksimović S, Tadić V, Arsić IA. The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique. in Acta Facultatis Medicae Naissensis. 2018;35(3):193-200.
doi:10.2478/afmnai-2018-0021 .

Stanković, Milica, Maksimović, Svetolik, Tadić, Vanja, Arsić, Ivana A., "The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique" in Acta Facultatis Medicae Naissensis, 35, no. 3 (2018):193-200,
https://doi.org/10.2478/afmnai-2018-0021 . .

TY  - JOUR
AU  - Bogdanović, Aleksandra
AU  - Tadić, Vanja
AU  - Arsić, Ivana A.
AU  - Milovanović, Stoja
AU  - Petrović, Slobodan
AU  - Skala, Dejan
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3406
AB  - Analysis of yield and composition profile of Melissa officinalis (Lemon balm) extract obtained by two-step CO2 extraction at high pressure was analyzed. The first or essential oil fraction, collected at 10 MPa and 40 degrees C, contained mostly the high volatile and aromatic compounds. The second fraction was consenquently extracted at 30 MPa using different temperature: 25 degrees C (higher pressure than critical pressure but at temperature below the critical; HPCP-CO2), 40 degrees C or 100 degrees C (supercritical CO2; SC-CO2). Obtained yield of essential oil fraction was 0.45% at 10 MPa, while depending on applied extraction temperature (HPCP-CO2 or SC-CO2), the yields of the second fractions collected at 30 MPa varied from 0.44% to 0.94%. Within the study, the content of active substances in obtained extracts was analyzed, too. For comparison with CO2 extracts (HPCP or SC) the yield and characterization of essential oil obtained by hydrodistillation was accomplished, as well. A significant difference was noted not only between the essential oil isolated by hydrodistillation and SC-CO2 extraction (40 degrees C, 10 MPa) but also between total extracts obtained by HPCP-CO2 and SC-CO2 extraction. The pseudo kinetic model was applied to define SC-CO2 extraction of Lemon balm to describe the relation between the yield of extraction and CO2 consumption. Antioxidant activity of obtained extracts was determined by free radical scavenging capacity (RSC) and significant correlation of antioxidant capacity and composition profile between obtained extracts was observed.
PB  - Elsevier, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Supercritical and high pressure subcritical fluid extraction from Lemon balm (Melissa officinalis L., Lamiaceae)
EP  - 242
SP  - 234
VL  - 107
DO  - 10.1016/j.supflu.2015.09.008
ER  - 

@article{
author = "Bogdanović, Aleksandra and Tadić, Vanja and Arsić, Ivana A. and Milovanović, Stoja and Petrović, Slobodan and Skala, Dejan",
year = "2016",
abstract = "Analysis of yield and composition profile of Melissa officinalis (Lemon balm) extract obtained by two-step CO2 extraction at high pressure was analyzed. The first or essential oil fraction, collected at 10 MPa and 40 degrees C, contained mostly the high volatile and aromatic compounds. The second fraction was consenquently extracted at 30 MPa using different temperature: 25 degrees C (higher pressure than critical pressure but at temperature below the critical; HPCP-CO2), 40 degrees C or 100 degrees C (supercritical CO2; SC-CO2). Obtained yield of essential oil fraction was 0.45% at 10 MPa, while depending on applied extraction temperature (HPCP-CO2 or SC-CO2), the yields of the second fractions collected at 30 MPa varied from 0.44% to 0.94%. Within the study, the content of active substances in obtained extracts was analyzed, too. For comparison with CO2 extracts (HPCP or SC) the yield and characterization of essential oil obtained by hydrodistillation was accomplished, as well. A significant difference was noted not only between the essential oil isolated by hydrodistillation and SC-CO2 extraction (40 degrees C, 10 MPa) but also between total extracts obtained by HPCP-CO2 and SC-CO2 extraction. The pseudo kinetic model was applied to define SC-CO2 extraction of Lemon balm to describe the relation between the yield of extraction and CO2 consumption. Antioxidant activity of obtained extracts was determined by free radical scavenging capacity (RSC) and significant correlation of antioxidant capacity and composition profile between obtained extracts was observed.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Supercritical and high pressure subcritical fluid extraction from Lemon balm (Melissa officinalis L., Lamiaceae)",
pages = "242-234",
volume = "107",
doi = "10.1016/j.supflu.2015.09.008"
}

Bogdanović, A., Tadić, V., Arsić, I. A., Milovanović, S., Petrović, S.,& Skala, D.. (2016). Supercritical and high pressure subcritical fluid extraction from Lemon balm (Melissa officinalis L., Lamiaceae). in Journal of Supercritical Fluids
Elsevier, Amsterdam., 107, 234-242.
https://doi.org/10.1016/j.supflu.2015.09.008

Bogdanović A, Tadić V, Arsić IA, Milovanović S, Petrović S, Skala D. Supercritical and high pressure subcritical fluid extraction from Lemon balm (Melissa officinalis L., Lamiaceae). in Journal of Supercritical Fluids. 2016;107:234-242.
doi:10.1016/j.supflu.2015.09.008 .

Bogdanović, Aleksandra, Tadić, Vanja, Arsić, Ivana A., Milovanović, Stoja, Petrović, Slobodan, Skala, Dejan, "Supercritical and high pressure subcritical fluid extraction from Lemon balm (Melissa officinalis L., Lamiaceae)" in Journal of Supercritical Fluids, 107 (2016):234-242,
https://doi.org/10.1016/j.supflu.2015.09.008 . .

TY  - JOUR
AU  - Roj, Edward
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Arsić, Ivana
AU  - Dobrzynska-Inger, Agnieszka
AU  - Kostrzewa, Dorota
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5797
AB  - Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 mu g mL(-1). Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of a-acids, beta-acids, iso-alpha-acids and xanthohumol in the samples were determined by HPLC.
PB  - De Gruyter Open Ltd, Warsaw
T2  - Open Chemistry
T1  - Supercritical carbon dioxide hops extracts with antimicrobial properties
EP  - 1171
IS  - 1
SP  - 1157
VL  - 13
DO  - 10.1515/chem-2015-0131
ER  - 

@article{
author = "Roj, Edward and Tadić, Vanja M. and Mišić, Dušan and Žižović, Irena and Arsić, Ivana and Dobrzynska-Inger, Agnieszka and Kostrzewa, Dorota",
year = "2015",
abstract = "Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 mu g mL(-1). Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of a-acids, beta-acids, iso-alpha-acids and xanthohumol in the samples were determined by HPLC.",
publisher = "De Gruyter Open Ltd, Warsaw",
journal = "Open Chemistry",
title = "Supercritical carbon dioxide hops extracts with antimicrobial properties",
pages = "1171-1157",
number = "1",
volume = "13",
doi = "10.1515/chem-2015-0131"
}

Roj, E., Tadić, V. M., Mišić, D., Žižović, I., Arsić, I., Dobrzynska-Inger, A.,& Kostrzewa, D.. (2015). Supercritical carbon dioxide hops extracts with antimicrobial properties. in Open Chemistry
De Gruyter Open Ltd, Warsaw., 13(1), 1157-1171.
https://doi.org/10.1515/chem-2015-0131

Roj E, Tadić VM, Mišić D, Žižović I, Arsić I, Dobrzynska-Inger A, Kostrzewa D. Supercritical carbon dioxide hops extracts with antimicrobial properties. in Open Chemistry. 2015;13(1):1157-1171.
doi:10.1515/chem-2015-0131 .

Roj, Edward, Tadić, Vanja M., Mišić, Dušan, Žižović, Irena, Arsić, Ivana, Dobrzynska-Inger, Agnieszka, Kostrzewa, Dorota, "Supercritical carbon dioxide hops extracts with antimicrobial properties" in Open Chemistry, 13, no. 1 (2015):1157-1171,
https://doi.org/10.1515/chem-2015-0131 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lukić, Milica Z.
AU  - Tasić-Kostov, Marija Z.
AU  - Tadić, Vanja
AU  - Arsić, Ivana A.
AU  - Mišić, Dušan
AU  - Petrović, Slobodan
AU  - Savić, Snežana D.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2883
AB  - Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.
AB  - Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation
T1  - Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije
EP  - 712
IS  - 6
SP  - 703
VL  - 69
DO  - 10.2298/HEMIND140701002Z
ER  - 

@article{
author = "Žugić, Ana R. and Lukić, Milica Z. and Tasić-Kostov, Marija Z. and Tadić, Vanja and Arsić, Ivana A. and Mišić, Dušan and Petrović, Slobodan and Savić, Snežana D.",
year = "2015",
abstract = "Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections., Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation, Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije",
pages = "712-703",
number = "6",
volume = "69",
doi = "10.2298/HEMIND140701002Z"
}

Žugić, A. R., Lukić, M. Z., Tasić-Kostov, M. Z., Tadić, V., Arsić, I. A., Mišić, D., Petrović, S.,& Savić, S. D.. (2015). Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija
Association of Chemical Engineers of Serbia., 69(6), 703-712.
https://doi.org/10.2298/HEMIND140701002Z

Žugić AR, Lukić MZ, Tasić-Kostov MZ, Tadić V, Arsić IA, Mišić D, Petrović S, Savić SD. Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija. 2015;69(6):703-712.
doi:10.2298/HEMIND140701002Z .

Žugić, Ana R., Lukić, Milica Z., Tasić-Kostov, Marija Z., Tadić, Vanja, Arsić, Ivana A., Mišić, Dušan, Petrović, Slobodan, Savić, Snežana D., "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation" in Hemijska industrija, 69, no. 6 (2015):703-712,
https://doi.org/10.2298/HEMIND140701002Z . .

TY  - JOUR
AU  - Arsić, Ivana A.
AU  - Tadić, Vanja
AU  - Đorđević, Sofija M.
AU  - Žugić, Ana R.
AU  - Vujić, Zorica
AU  - Petrović, Slobodan
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2639
AB  - Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99).
AB  - Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Optimization of extraction of antioxidant components from yarrow herb
T1  - Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave
EP  - 517
IS  - 4
SP  - 511
VL  - 68
DO  - 10.2298/HEMIND130910076A
ER  - 

@article{
author = "Arsić, Ivana A. and Tadić, Vanja and Đorđević, Sofija M. and Žugić, Ana R. and Vujić, Zorica and Petrović, Slobodan",
year = "2014",
abstract = "Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99)., Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Optimization of extraction of antioxidant components from yarrow herb, Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave",
pages = "517-511",
number = "4",
volume = "68",
doi = "10.2298/HEMIND130910076A"
}

Arsić, I. A., Tadić, V., Đorđević, S. M., Žugić, A. R., Vujić, Z.,& Petrović, S.. (2014). Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija
Association of Chemical Engineers of Serbia., 68(4), 511-517.
https://doi.org/10.2298/HEMIND130910076A

Arsić IA, Tadić V, Đorđević SM, Žugić AR, Vujić Z, Petrović S. Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija. 2014;68(4):511-517.
doi:10.2298/HEMIND130910076A .

Arsić, Ivana A., Tadić, Vanja, Đorđević, Sofija M., Žugić, Ana R., Vujić, Zorica, Petrović, Slobodan, "Optimization of extraction of antioxidant components from yarrow herb" in Hemijska industrija, 68, no. 4 (2014):511-517,
https://doi.org/10.2298/HEMIND130910076A . .

TY  - JOUR
AU  - Tadić, V.
AU  - Đorđević, S.
AU  - Žugić, Ana R.
AU  - Arsić, Ivana A.
AU  - Bogdanović, Aleksandra
AU  - Homsek, Irena J.
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2318
PB  - Elsevier Science Bv, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - Sensitive HPLC Method for the Determination of Usnic Acid in Tablets
EP  - E206
SP  - E206
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2318
ER  - 

@article{
author = "Tadić, V. and Đorđević, S. and Žugić, Ana R. and Arsić, Ivana A. and Bogdanović, Aleksandra and Homsek, Irena J.",
year = "2013",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Sensitive HPLC Method for the Determination of Usnic Acid in Tablets",
pages = "E206-E206",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2318"
}

Tadić, V., Đorđević, S., Žugić, A. R., Arsić, I. A., Bogdanović, A.,& Homsek, I. J.. (2013). Sensitive HPLC Method for the Determination of Usnic Acid in Tablets. in European Journal of Pharmaceutical Sciences
Elsevier Science Bv, Amsterdam., 50, E206-E206.
https://hdl.handle.net/21.15107/rcub_technorep_2318

Tadić V, Đorđević S, Žugić AR, Arsić IA, Bogdanović A, Homsek IJ. Sensitive HPLC Method for the Determination of Usnic Acid in Tablets. in European Journal of Pharmaceutical Sciences. 2013;50:E206-E206.
https://hdl.handle.net/21.15107/rcub_technorep_2318 .

Tadić, V., Đorđević, S., Žugić, Ana R., Arsić, Ivana A., Bogdanović, Aleksandra, Homsek, Irena J., "Sensitive HPLC Method for the Determination of Usnic Acid in Tablets" in European Journal of Pharmaceutical Sciences, 50 (2013):E206-E206,
https://hdl.handle.net/21.15107/rcub_technorep_2318 .