TY  - JOUR
AU  - Lukic, Ivana
AU  - Pajnik, Jelena
AU  - Tadic, Vanja
AU  - Milovanovic, Stoja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5146
AB  - The aim of the present study was the utilization of supercritical CO2 as a green medium in various processes: starch gel drying, supercritical extraction from hemp seed flour (SCE process), hemp seed oil (HSO) impregnation (SCI process), as well as integrated process of hemp seed flour (HSF) extraction and starch gels impregnation (SCE-SCI process) for development of added-value materials that can be used as phytopharmaceuticals. Optimization of starch aerogels was performed by variation of temperature (35 and 45 °C) and pressure (8, 10, and 20 MPa) in order to obtain materials with high porosity, which will enable maximal loading capacity for hemp seed extracts. Proposed scCO2-assisted processes of SCE from HSF, SCI and SCE-SCI were performed at pressure of 30 MPa and temperature of 60 °C. It was shown that conditions of starch gel drying significantly influenced material morphology (porosity was in a range of 48-82%, and specific surface area of 71-208 m2/g), which consequently determined aerogel loading capacity. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after drying process. The highest loading of both HSO and HSF extract (24.9% and 29.78%, respectively) was achieved when aerogel obtained at 10 MPa and 45 °C was tested as a carrier. Furthermore, chemical analysis showed that both HSO and HSF extract are rich in unsaturated fatty acids especially linoleic acid (54-59%) and α-linolenic acid (15-18%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases.
PB  - Elsevier Ltd
T2  - Journal of CO2 Utilization
T1  - Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation
SP  - 102036
VL  - 61
DO  - 10.1016/j.jcou.2022.102036
ER  - 

@article{
author = "Lukic, Ivana and Pajnik, Jelena and Tadic, Vanja and Milovanovic, Stoja",
year = "2022",
abstract = "The aim of the present study was the utilization of supercritical CO2 as a green medium in various processes: starch gel drying, supercritical extraction from hemp seed flour (SCE process), hemp seed oil (HSO) impregnation (SCI process), as well as integrated process of hemp seed flour (HSF) extraction and starch gels impregnation (SCE-SCI process) for development of added-value materials that can be used as phytopharmaceuticals. Optimization of starch aerogels was performed by variation of temperature (35 and 45 °C) and pressure (8, 10, and 20 MPa) in order to obtain materials with high porosity, which will enable maximal loading capacity for hemp seed extracts. Proposed scCO2-assisted processes of SCE from HSF, SCI and SCE-SCI were performed at pressure of 30 MPa and temperature of 60 °C. It was shown that conditions of starch gel drying significantly influenced material morphology (porosity was in a range of 48-82%, and specific surface area of 71-208 m2/g), which consequently determined aerogel loading capacity. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after drying process. The highest loading of both HSO and HSF extract (24.9% and 29.78%, respectively) was achieved when aerogel obtained at 10 MPa and 45 °C was tested as a carrier. Furthermore, chemical analysis showed that both HSO and HSF extract are rich in unsaturated fatty acids especially linoleic acid (54-59%) and α-linolenic acid (15-18%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases.",
publisher = "Elsevier Ltd",
journal = "Journal of CO2 Utilization",
title = "Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation",
pages = "102036",
volume = "61",
doi = "10.1016/j.jcou.2022.102036"
}

Lukic, I., Pajnik, J., Tadic, V.,& Milovanovic, S.. (2022). Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation. in Journal of CO2 Utilization
Elsevier Ltd., 61, 102036.
https://doi.org/10.1016/j.jcou.2022.102036

Lukic I, Pajnik J, Tadic V, Milovanovic S. Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation. in Journal of CO2 Utilization. 2022;61:102036.
doi:10.1016/j.jcou.2022.102036 .

Lukic, Ivana, Pajnik, Jelena, Tadic, Vanja, Milovanovic, Stoja, "Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation" in Journal of CO2 Utilization, 61 (2022):102036,
https://doi.org/10.1016/j.jcou.2022.102036 . .

TY  - JOUR
AU  - Milovanovic, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Dębczak, Agnieszka
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5163
AB  - The present study was intended to explore dandelion seeds as a new source of biologically active constituents using an environmentally friendly extraction technique. The process-function relationships were determined by following the influence of temperature (40 and 60 °C) and pressure (from 100 to 450 bar) on the amount of separated extract, its density, composition, and bioactivity. It was shown that the supercritical CO2-assisted technique enables the separation of 0.6–25.0% extract having a density in a range of 827–941 kg/m3. Extracts contained a high amount of unsaturated fatty acids (89.9–90.6%) including linoleic acid (71%) and α-linolenic acid (up to 0.9%), as well as phenolic (5.5–12.1 mg GAE/g) and flavonoid compounds (208.6–564.5 μg QE/g). The antioxidant activity test showed that 20 mg/mL extract solutions inhibit 26.0–64.4% DPPH radical. In addition, extracts showed significant antimicrobial activity, especially against Gram-positive Bacillus subtilis and Micrococcus luteus. There was no cytotoxic effect on normal green monkey kidney cells and human skin fibroblasts nor on hypopharyngeal cancer, cervical adenocarcinoma, and colon cancer cells. The study indicated the possible safe application of bioactive dandelion seeds extract in the pharmaceutical, food, and cosmetic industry.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique
SP  - 100796
VL  - 29
DO  - 10.1016/j.scp.2022.100796
ER  - 

@article{
author = "Milovanovic, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Dębczak, Agnieszka and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The present study was intended to explore dandelion seeds as a new source of biologically active constituents using an environmentally friendly extraction technique. The process-function relationships were determined by following the influence of temperature (40 and 60 °C) and pressure (from 100 to 450 bar) on the amount of separated extract, its density, composition, and bioactivity. It was shown that the supercritical CO2-assisted technique enables the separation of 0.6–25.0% extract having a density in a range of 827–941 kg/m3. Extracts contained a high amount of unsaturated fatty acids (89.9–90.6%) including linoleic acid (71%) and α-linolenic acid (up to 0.9%), as well as phenolic (5.5–12.1 mg GAE/g) and flavonoid compounds (208.6–564.5 μg QE/g). The antioxidant activity test showed that 20 mg/mL extract solutions inhibit 26.0–64.4% DPPH radical. In addition, extracts showed significant antimicrobial activity, especially against Gram-positive Bacillus subtilis and Micrococcus luteus. There was no cytotoxic effect on normal green monkey kidney cells and human skin fibroblasts nor on hypopharyngeal cancer, cervical adenocarcinoma, and colon cancer cells. The study indicated the possible safe application of bioactive dandelion seeds extract in the pharmaceutical, food, and cosmetic industry.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique",
pages = "100796",
volume = "29",
doi = "10.1016/j.scp.2022.100796"
}

Milovanovic, S., Grzegorczyk, A., Świątek, Ł., Dębczak, A., Tyskiewicz, K.,& Konkol, M.. (2022). Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 29, 100796.
https://doi.org/10.1016/j.scp.2022.100796

Milovanovic S, Grzegorczyk A, Świątek Ł, Dębczak A, Tyskiewicz K, Konkol M. Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique. in Sustainable Chemistry and Pharmacy. 2022;29:100796.
doi:10.1016/j.scp.2022.100796 .

Milovanovic, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Dębczak, Agnieszka, Tyskiewicz, Katarzyna, Konkol, Marcin, "Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique" in Sustainable Chemistry and Pharmacy, 29 (2022):100796,
https://doi.org/10.1016/j.scp.2022.100796 . .

TY  - JOUR
AU  - Djuris, Jelena
AU  - Milovanovic, Stoja
AU  - Medarevic, Djordje
AU  - Dobricic, Vladimir
AU  - Dapčević, Aleksandra
AU  - Ibric, Svetlana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5030
AB  - Solid dispersions production is one of the substantial approaches for improvement of poor drug solubility. Additionally, supercritical fluid assisted method for preparation of solid dispersions can offer many advantages in comparison to the conventional melting or solvent-evaporation methods. Miscibility analysis provides valuable guidance for selection of the most appropriate polymeric carrier for dispersion of the drug of interest. In addition to the increased drug release rate, solid dispersions should have proper mechanical attributes in order to be successfully formulated in the final solid dosage form such as tablet. Therefore, several pharmaceutical grade polymers have been selected for development of BCS Class II drug carvedilol (CARV) solid dispersions. They were compared based on behavior in supercritical CO 2 and affinity towards CARV calculated from the miscibility analysis. By utilization of the supercritical CO 2 assisted method, solid dispersions of CARV with the selected (co)polymers (polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), Soluplus® and Eudragit®) were obtained. Properties of the prepared CARV-polymer dispersions were observed by the polarizing and scanning electron microscopy and analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. CARV was additionally characterized by X-ray powder diffraction. Furthermore, in vitro dissolution studies and dynamic compaction analysis were performed on the selected samples of solid dispersions. Among the studied polymers, PVP and HPMC have been identified as polymers with the highest affinity towards CARV, based on the calculated δ p values. This has been also confirmed with the highest dissolution efficiency of CARV-PVP and CARV-HPMC solid dispersions. Solid state characterization indicated that CARV was dispersed either molecularly, or in the amorphous form, depending on interactions with each polymer. Determination of CARV-PVP and CARV-HPMC mechanical properties revealed that CARV-PVP solid dispersion has superior compactibility and tabletability. Therefore, CARV-PVP solid dispersion has been highlighted as the most appropriate for the further development of tablets as the final dosage form. Presented study provides an example for efficient approach for development of poorly soluble drug solid dispersion with satisfactory tableting properties.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions
EP  - 200
SP  - 190
VL  - 554
DO  - 10.1016/j.ijpharm.2018.11.015
ER  - 

@article{
author = "Djuris, Jelena and Milovanovic, Stoja and Medarevic, Djordje and Dobricic, Vladimir and Dapčević, Aleksandra and Ibric, Svetlana",
year = "2019",
abstract = "Solid dispersions production is one of the substantial approaches for improvement of poor drug solubility. Additionally, supercritical fluid assisted method for preparation of solid dispersions can offer many advantages in comparison to the conventional melting or solvent-evaporation methods. Miscibility analysis provides valuable guidance for selection of the most appropriate polymeric carrier for dispersion of the drug of interest. In addition to the increased drug release rate, solid dispersions should have proper mechanical attributes in order to be successfully formulated in the final solid dosage form such as tablet. Therefore, several pharmaceutical grade polymers have been selected for development of BCS Class II drug carvedilol (CARV) solid dispersions. They were compared based on behavior in supercritical CO 2 and affinity towards CARV calculated from the miscibility analysis. By utilization of the supercritical CO 2 assisted method, solid dispersions of CARV with the selected (co)polymers (polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), Soluplus® and Eudragit®) were obtained. Properties of the prepared CARV-polymer dispersions were observed by the polarizing and scanning electron microscopy and analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. CARV was additionally characterized by X-ray powder diffraction. Furthermore, in vitro dissolution studies and dynamic compaction analysis were performed on the selected samples of solid dispersions. Among the studied polymers, PVP and HPMC have been identified as polymers with the highest affinity towards CARV, based on the calculated δ p values. This has been also confirmed with the highest dissolution efficiency of CARV-PVP and CARV-HPMC solid dispersions. Solid state characterization indicated that CARV was dispersed either molecularly, or in the amorphous form, depending on interactions with each polymer. Determination of CARV-PVP and CARV-HPMC mechanical properties revealed that CARV-PVP solid dispersion has superior compactibility and tabletability. Therefore, CARV-PVP solid dispersion has been highlighted as the most appropriate for the further development of tablets as the final dosage form. Presented study provides an example for efficient approach for development of poorly soluble drug solid dispersion with satisfactory tableting properties.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions",
pages = "200-190",
volume = "554",
doi = "10.1016/j.ijpharm.2018.11.015"
}

Djuris, J., Milovanovic, S., Medarevic, D., Dobricic, V., Dapčević, A.,& Ibric, S.. (2019). Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions. in International Journal of Pharmaceutics
Elsevier B.V.., 554, 190-200.
https://doi.org/10.1016/j.ijpharm.2018.11.015

Djuris J, Milovanovic S, Medarevic D, Dobricic V, Dapčević A, Ibric S. Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions. in International Journal of Pharmaceutics. 2019;554:190-200.
doi:10.1016/j.ijpharm.2018.11.015 .

Djuris, Jelena, Milovanovic, Stoja, Medarevic, Djordje, Dobricic, Vladimir, Dapčević, Aleksandra, Ibric, Svetlana, "Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions" in International Journal of Pharmaceutics, 554 (2019):190-200,
https://doi.org/10.1016/j.ijpharm.2018.11.015 . .