TY  - JOUR
AU  - Mijin, Nemanja
AU  - Milošević, Jelica
AU  - Stevanović, Sanja
AU  - Petrović, Predrag
AU  - Lolić, Aleksandar
AU  - Urbic, Tomaz
AU  - Polović, Natalija
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5268
AB  - The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.
PB  - Elsevier B.V.
T2  - Food Hydrocolloids
T1  - Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin
SP  - 108292
VL  - 136
DO  - 10.1016/j.foodhyd.2022.108292
ER  - 

@article{
author = "Mijin, Nemanja and Milošević, Jelica and Stevanović, Sanja and Petrović, Predrag and Lolić, Aleksandar and Urbic, Tomaz and Polović, Natalija",
year = "2023",
abstract = "The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.",
publisher = "Elsevier B.V.",
journal = "Food Hydrocolloids",
title = "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin",
pages = "108292",
volume = "136",
doi = "10.1016/j.foodhyd.2022.108292"
}

Mijin, N., Milošević, J., Stevanović, S., Petrović, P., Lolić, A., Urbic, T.,& Polović, N.. (2023). Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids
Elsevier B.V.., 136, 108292.
https://doi.org/10.1016/j.foodhyd.2022.108292

Mijin N, Milošević J, Stevanović S, Petrović P, Lolić A, Urbic T, Polović N. Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids. 2023;136:108292.
doi:10.1016/j.foodhyd.2022.108292 .

Mijin, Nemanja, Milošević, Jelica, Stevanović, Sanja, Petrović, Predrag, Lolić, Aleksandar, Urbic, Tomaz, Polović, Natalija, "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin" in Food Hydrocolloids, 136 (2023):108292,
https://doi.org/10.1016/j.foodhyd.2022.108292 . .

TY  - CONF
AU  - Radenković, Mirjana
AU  - Milošević, Sanja
AU  - Stanković, Srboljub
AU  - Joksić, J.
AU  - Onjia, Antonije
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7099
AB  - Natural uranium, consisting of 238U, 234U and 235U isotopes is present in the environment in low
concentrations especially in the atmosphere where it usually exists as a constituent of particulate
matter submicron conglomerates suspended in the air. In the case of contamination, uranium may be
present in the aerosol, being transferred to other areas by wind and again settled on the surface soil
with possible migration into deeper layers or resuspension under certain meteorological conditions.
Here results on the airborne uranium assessment based on the analysis of lichen species already
present or transplanted into contaminated areas will be presented. With that aim, different in situ
and transplanted epiphytic lichen species have been taken at selected locations in southern Serbia in
the stage of existing contamination by depleted uranium, during the clean-up activities and
afterwards, in all four seasons. Collected samples underwent analysis by sensitive nuclear analytical
techniques. The INAA and ICP MS results are derived from 238U mass fraction while high resolution
alpha-spectrometry gave results for each isotope expressed as specific activity concentration
(Bq kg-1). Based on the isotopic ratios 235U /238U and 234U /238U, depleted uranium content was
possible to distinguish from natural uranium in the samples. Results have shown that the sensitivity
of the examined morphologically different lichen types and their ability to accumulate metals
including uranium, strongly depend on metals’ concentration in the air as well as on the age of
lichens, properties of host species, chemical properties of particles, local climate conditions, exposure
time etc. Epiphytic lichen species Evernia prunastri was found to be the most suitable bioindicator
for the accumulation of uranium airborne particles. Concerning the uranium content, a significant
difference in concentrations had been observed for different sampling phases, with maximum
depleted uranium contribution during the clean-up activities. The variability in uranium
concentrations was noticeable in relation to prevailing wind direction, position and distance of the
sampling points. Having in mind the results obtained within the national radioactivity monitoring
program 2011-2017, it may be concluded that for detailed airborne uranium assessment, a
methodology such as biomonitoring with the application of sensitive nuclear techniques should be
considered.
PB  - Niš : RAD Centre
C3  - Book of abstracts / Seventh International Conference on Radiation in Various Fields of Research, RAD 7, RAD 2019, 10-14.06.2019 Herceg Novi, Montenegro
T1  - Airborne uranium assessment by epiphytic lichen species in contaminated areas
SP  - 419
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7099
ER  - 

@conference{
author = "Radenković, Mirjana and Milošević, Sanja and Stanković, Srboljub and Joksić, J. and Onjia, Antonije",
year = "2019",
abstract = "Natural uranium, consisting of 238U, 234U and 235U isotopes is present in the environment in low
concentrations especially in the atmosphere where it usually exists as a constituent of particulate
matter submicron conglomerates suspended in the air. In the case of contamination, uranium may be
present in the aerosol, being transferred to other areas by wind and again settled on the surface soil
with possible migration into deeper layers or resuspension under certain meteorological conditions.
Here results on the airborne uranium assessment based on the analysis of lichen species already
present or transplanted into contaminated areas will be presented. With that aim, different in situ
and transplanted epiphytic lichen species have been taken at selected locations in southern Serbia in
the stage of existing contamination by depleted uranium, during the clean-up activities and
afterwards, in all four seasons. Collected samples underwent analysis by sensitive nuclear analytical
techniques. The INAA and ICP MS results are derived from 238U mass fraction while high resolution
alpha-spectrometry gave results for each isotope expressed as specific activity concentration
(Bq kg-1). Based on the isotopic ratios 235U /238U and 234U /238U, depleted uranium content was
possible to distinguish from natural uranium in the samples. Results have shown that the sensitivity
of the examined morphologically different lichen types and their ability to accumulate metals
including uranium, strongly depend on metals’ concentration in the air as well as on the age of
lichens, properties of host species, chemical properties of particles, local climate conditions, exposure
time etc. Epiphytic lichen species Evernia prunastri was found to be the most suitable bioindicator
for the accumulation of uranium airborne particles. Concerning the uranium content, a significant
difference in concentrations had been observed for different sampling phases, with maximum
depleted uranium contribution during the clean-up activities. The variability in uranium
concentrations was noticeable in relation to prevailing wind direction, position and distance of the
sampling points. Having in mind the results obtained within the national radioactivity monitoring
program 2011-2017, it may be concluded that for detailed airborne uranium assessment, a
methodology such as biomonitoring with the application of sensitive nuclear techniques should be
considered.",
publisher = "Niš : RAD Centre",
journal = "Book of abstracts / Seventh International Conference on Radiation in Various Fields of Research, RAD 7, RAD 2019, 10-14.06.2019 Herceg Novi, Montenegro",
title = "Airborne uranium assessment by epiphytic lichen species in contaminated areas",
pages = "419",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7099"
}

Radenković, M., Milošević, S., Stanković, S., Joksić, J.,& Onjia, A.. (2019). Airborne uranium assessment by epiphytic lichen species in contaminated areas. in Book of abstracts / Seventh International Conference on Radiation in Various Fields of Research, RAD 7, RAD 2019, 10-14.06.2019 Herceg Novi, Montenegro
Niš : RAD Centre., 419.
https://hdl.handle.net/21.15107/rcub_technorep_7099

Radenković M, Milošević S, Stanković S, Joksić J, Onjia A. Airborne uranium assessment by epiphytic lichen species in contaminated areas. in Book of abstracts / Seventh International Conference on Radiation in Various Fields of Research, RAD 7, RAD 2019, 10-14.06.2019 Herceg Novi, Montenegro. 2019;:419.
https://hdl.handle.net/21.15107/rcub_technorep_7099 .

Radenković, Mirjana, Milošević, Sanja, Stanković, Srboljub, Joksić, J., Onjia, Antonije, "Airborne uranium assessment by epiphytic lichen species in contaminated areas" in Book of abstracts / Seventh International Conference on Radiation in Various Fields of Research, RAD 7, RAD 2019, 10-14.06.2019 Herceg Novi, Montenegro (2019):419,
https://hdl.handle.net/21.15107/rcub_technorep_7099 .