TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Andrejevic, Milica
AU  - Janaćković, Đorđe
AU  - Obradović, Bojana
AU  - Veljović, Đorđe
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4934
AB  - In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.
T2  - Journal of Biomedical Materials Research Part B-Applied Biomaterials
T1  - Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions
EP  - 2090
IS  - 12
SP  - 2079
VL  - 109
DO  - 10.1002/jbm.b.34856
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Andrejevic, Milica and Janaćković, Đorđe and Obradović, Bojana and Veljović, Đorđe",
year = "2021",
abstract = "In the present study, we synthesized hydroxyapatite (HAP) powders followed by the production of alginate based macroporous scaffolds with the aim to imitate the natural bone structure. HAP powders were synthesized by using a hydrothermal method, and after calcination, dominant phases in the powders, undoped and doped with Mg2+ were HAP and beta-tricalcium phosphate, respectively. Upon mixing with Na-alginate, followed by gelation and freeze-dying, highly macroporous composite scaffolds were obtained with open and connected pores and uniformly dispersed mineral phase as determined by scanning electron microscopy. Mechanical properties of the scaffolds were influenced by the composition of calcium phosphate fillers being improved as Ca2+ concentration increased while Mg2+ concentration decreased. HAP formation within all scaffolds was investigated in simulated body fluid (SBF) during 28 days under static conditions while the best candidate (Mg substituted HAP filler, precursor solution with [Ca + Mg]/P molar ratio of 1.52) was investigated under more physiological conditions in a biomimetic perfusion bioreactor. The continuous SBF flow (superficial velocity of 400 mu m/s) induced the formation of abundant HAP crystals throughout the scaffolds leading to improved mechanical properties to some extent as compared to the initial scaffolds. These findings indicated potentials of novel biomimetic scaffolds for use in bone tissue engineering.",
journal = "Journal of Biomedical Materials Research Part B-Applied Biomaterials",
title = "Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions",
pages = "2090-2079",
number = "12",
volume = "109",
doi = "10.1002/jbm.b.34856"
}

Stojkovska, J., Zvicer, J., Andrejevic, M., Janaćković, Đ., Obradović, B.,& Veljović, Đ.. (2021). Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions. in Journal of Biomedical Materials Research Part B-Applied Biomaterials, 109(12), 2079-2090.
https://doi.org/10.1002/jbm.b.34856

Stojkovska J, Zvicer J, Andrejevic M, Janaćković Đ, Obradović B, Veljović Đ. Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions. in Journal of Biomedical Materials Research Part B-Applied Biomaterials. 2021;109(12):2079-2090.
doi:10.1002/jbm.b.34856 .

Stojkovska, Jasmina, Zvicer, Jovana, Andrejevic, Milica, Janaćković, Đorđe, Obradović, Bojana, Veljović, Đorđe, "Novel composite scaffolds based on alginate and Mg-doped calcium phosphate fillers: Enhanced hydroxyapatite formation under biomimetic conditions" in Journal of Biomedical Materials Research Part B-Applied Biomaterials, 109, no. 12 (2021):2079-2090,
https://doi.org/10.1002/jbm.b.34856 . .