TY  - CONF
AU  - Milošević, Mia
AU  - Banićević, Ivana
AU  - Pavlović, Marija
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6351
PB  - Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
T1  - Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6351
ER  - 

@conference{
author = "Milošević, Mia and Banićević, Ivana and Pavlović, Marija and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts",
title = "Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6351"
}

Milošević, M., Banićević, I., Pavlović, M., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts
Institute of Technical Sciences of SASA., 8.
https://hdl.handle.net/21.15107/rcub_technorep_6351

Milošević M, Banićević I, Pavlović M, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor. in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts. 2022;:8.
https://hdl.handle.net/21.15107/rcub_technorep_6351 .

Milošević, Mia, Banićević, Ivana, Pavlović, Marija, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Development of a physiologically relevant osteosarcoma model based on alginate scaffolds and perfusion bioreactor" in Twentieth Young Researchers’ Conference - Materials Science and Engineering: Programme and the Book of Abstracts (2022):8,
https://hdl.handle.net/21.15107/rcub_technorep_6351 .

TY  - CONF
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6354
AB  - Cancer cell immobilization in polymer hydrogels serving as extracellular matrices and cultivation in perfusion bioreactors
that provide appropriate chemical signals, efficient mass transfer and hydrodynamic shear stresses is a promising strategy
for development of physiologically relevant tumor models. In this work, perfusion cultures of 2 cancer cell types (C6 rat
glioma and embryonal carcinoma NT2/D1 cells) immobilized in alginate microgels were established, while static cultures
served as controls. Continuous perfusion had different effects on the cultured cells inducing enhanced proliferation of the
glioma cells immobilized in microfibers (8x10^6 cell/ml), while reducing the viability of the NT2/D1 cells immobilized in
microbeads (1x10^6 cell/ml). In order to elucidate the observed effects, chemical engineering principles were applied to
assess mass transfer and hydrodynamic conditions. The second Fick’s law was solved analytically while the diffusionadvection-reaction equation was solved numerically to model mass transport in the static and bioreactor cultures,
respectively. Moreover, Reynolds numbers, pressure drops and shear stresses in bioreactor cultures were calculated for
assessment of flow regime and hydrodynamic conditions. The modeling results have indicated that oxygen transport is
diffusion-controlled through the alginate hydrogel, while medium perfusion improves mass transfer of larger compounds
having smaller diffusion coefficients (∼10^(-13) m^2/s), which possibly stimulated glioma cell proliferation. On the other
hand, the obtained shear stress (~50 mPa) in the perfused packed bed of microbeads was above physiological levels, which
provided the explanation of the poor NT2/D1 cell survival. This study stresses the importance of multidisciplinary approach
in addressing such multifactorial diseases as cancer.
C3  - 6th World TERMIS Conference 2021 Abstract Book
T1  - Optimization of 3D cancer cell culture conditions by application of chemical engineering principles
SP  - 1408
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6354
ER  - 

@conference{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2021",
abstract = "Cancer cell immobilization in polymer hydrogels serving as extracellular matrices and cultivation in perfusion bioreactors
that provide appropriate chemical signals, efficient mass transfer and hydrodynamic shear stresses is a promising strategy
for development of physiologically relevant tumor models. In this work, perfusion cultures of 2 cancer cell types (C6 rat
glioma and embryonal carcinoma NT2/D1 cells) immobilized in alginate microgels were established, while static cultures
served as controls. Continuous perfusion had different effects on the cultured cells inducing enhanced proliferation of the
glioma cells immobilized in microfibers (8x10^6 cell/ml), while reducing the viability of the NT2/D1 cells immobilized in
microbeads (1x10^6 cell/ml). In order to elucidate the observed effects, chemical engineering principles were applied to
assess mass transfer and hydrodynamic conditions. The second Fick’s law was solved analytically while the diffusionadvection-reaction equation was solved numerically to model mass transport in the static and bioreactor cultures,
respectively. Moreover, Reynolds numbers, pressure drops and shear stresses in bioreactor cultures were calculated for
assessment of flow regime and hydrodynamic conditions. The modeling results have indicated that oxygen transport is
diffusion-controlled through the alginate hydrogel, while medium perfusion improves mass transfer of larger compounds
having smaller diffusion coefficients (∼10^(-13) m^2/s), which possibly stimulated glioma cell proliferation. On the other
hand, the obtained shear stress (~50 mPa) in the perfused packed bed of microbeads was above physiological levels, which
provided the explanation of the poor NT2/D1 cell survival. This study stresses the importance of multidisciplinary approach
in addressing such multifactorial diseases as cancer.",
journal = "6th World TERMIS Conference 2021 Abstract Book",
title = "Optimization of 3D cancer cell culture conditions by application of chemical engineering principles",
pages = "1408",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6354"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2021). Optimization of 3D cancer cell culture conditions by application of chemical engineering principles. in 6th World TERMIS Conference 2021 Abstract Book, 1408.
https://hdl.handle.net/21.15107/rcub_technorep_6354

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Optimization of 3D cancer cell culture conditions by application of chemical engineering principles. in 6th World TERMIS Conference 2021 Abstract Book. 2021;:1408.
https://hdl.handle.net/21.15107/rcub_technorep_6354 .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of 3D cancer cell culture conditions by application of chemical engineering principles" in 6th World TERMIS Conference 2021 Abstract Book (2021):1408,
https://hdl.handle.net/21.15107/rcub_technorep_6354 .