TY  - JOUR
AU  - Lukic, Ivana
AU  - Pajnik, Jelena
AU  - Tadic, Vanja
AU  - Milovanovic, Stoja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5146
AB  - The aim of the present study was the utilization of supercritical CO2 as a green medium in various processes: starch gel drying, supercritical extraction from hemp seed flour (SCE process), hemp seed oil (HSO) impregnation (SCI process), as well as integrated process of hemp seed flour (HSF) extraction and starch gels impregnation (SCE-SCI process) for development of added-value materials that can be used as phytopharmaceuticals. Optimization of starch aerogels was performed by variation of temperature (35 and 45 °C) and pressure (8, 10, and 20 MPa) in order to obtain materials with high porosity, which will enable maximal loading capacity for hemp seed extracts. Proposed scCO2-assisted processes of SCE from HSF, SCI and SCE-SCI were performed at pressure of 30 MPa and temperature of 60 °C. It was shown that conditions of starch gel drying significantly influenced material morphology (porosity was in a range of 48-82%, and specific surface area of 71-208 m2/g), which consequently determined aerogel loading capacity. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after drying process. The highest loading of both HSO and HSF extract (24.9% and 29.78%, respectively) was achieved when aerogel obtained at 10 MPa and 45 °C was tested as a carrier. Furthermore, chemical analysis showed that both HSO and HSF extract are rich in unsaturated fatty acids especially linoleic acid (54-59%) and α-linolenic acid (15-18%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases.
PB  - Elsevier Ltd
T2  - Journal of CO2 Utilization
T1  - Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation
SP  - 102036
VL  - 61
DO  - 10.1016/j.jcou.2022.102036
ER  - 

@article{
author = "Lukic, Ivana and Pajnik, Jelena and Tadic, Vanja and Milovanovic, Stoja",
year = "2022",
abstract = "The aim of the present study was the utilization of supercritical CO2 as a green medium in various processes: starch gel drying, supercritical extraction from hemp seed flour (SCE process), hemp seed oil (HSO) impregnation (SCI process), as well as integrated process of hemp seed flour (HSF) extraction and starch gels impregnation (SCE-SCI process) for development of added-value materials that can be used as phytopharmaceuticals. Optimization of starch aerogels was performed by variation of temperature (35 and 45 °C) and pressure (8, 10, and 20 MPa) in order to obtain materials with high porosity, which will enable maximal loading capacity for hemp seed extracts. Proposed scCO2-assisted processes of SCE from HSF, SCI and SCE-SCI were performed at pressure of 30 MPa and temperature of 60 °C. It was shown that conditions of starch gel drying significantly influenced material morphology (porosity was in a range of 48-82%, and specific surface area of 71-208 m2/g), which consequently determined aerogel loading capacity. FTIR analysis confirmed that scCO2 did not have effect on polymer composition nor it remained in polymer after drying process. The highest loading of both HSO and HSF extract (24.9% and 29.78%, respectively) was achieved when aerogel obtained at 10 MPa and 45 °C was tested as a carrier. Furthermore, chemical analysis showed that both HSO and HSF extract are rich in unsaturated fatty acids especially linoleic acid (54-59%) and α-linolenic acid (15-18%). These essential fatty acids have well-established health benefits including protection against cardiovascular, neurodegenerative and inflammatory diseases.",
publisher = "Elsevier Ltd",
journal = "Journal of CO2 Utilization",
title = "Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation",
pages = "102036",
volume = "61",
doi = "10.1016/j.jcou.2022.102036"
}

Lukic, I., Pajnik, J., Tadic, V.,& Milovanovic, S.. (2022). Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation. in Journal of CO2 Utilization
Elsevier Ltd., 61, 102036.
https://doi.org/10.1016/j.jcou.2022.102036

Lukic I, Pajnik J, Tadic V, Milovanovic S. Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation. in Journal of CO2 Utilization. 2022;61:102036.
doi:10.1016/j.jcou.2022.102036 .

Lukic, Ivana, Pajnik, Jelena, Tadic, Vanja, Milovanovic, Stoja, "Supercritical CO2-assisted processes for development of added-value materials: Optimization of starch aerogels preparation and hemp seed extracts impregnation" in Journal of CO2 Utilization, 61 (2022):102036,
https://doi.org/10.1016/j.jcou.2022.102036 . .

TY  - JOUR
AU  - Tadic, Vanja M.
AU  - Zugic, Ana
AU  - Martinovic, Milica
AU  - Stankovic, Milica
AU  - Maksimović, Svetolik
AU  - Frank, Almut
AU  - Nesic, Ivana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4821
AB  - Immortelle, as rich source of chlorogenic acid and the phloroglucinol alpha-pyrone compound arzanol, possesses anti-inflammatory and antioxidant properties, affects cell regeneration, and has positive effect on many skin conditions. Hemp oil, characterized by a favorable omega-6 to omega-3 ratio, as well as an abundance of essential fatty acids and vitamin E, participates in immunoregulation and also act as an anti-inflammatory. In the present study, we examined the effect on the skin of creams and emulgels with immortelle extract and hemp oil, by comparing them to placebo samples and a non-treated control. A long-term in vivo study of biophysical skin characteristics, which lasted for 30 days, was conducted on 25 healthy human volunteers. Measured parameters were electrical capacitance of the stratum corneum, trans-epidermal water loss (TEWL), and skin pH and erythema index. Further, a sensory study was carried out in which the panelists had to choose descriptive terms for sensory attributes in questionnaire. The results showed that application of all preparations led to increase of skin hydration and TEWL reduction, while the skin was not irritated, and its normal pH was not disrupted. This study also showed importance of the carrier. Not only were emulgels described by panelists as preparations with better sensory properties, there was a significant difference between the skin hydration effect of emulgel with immortelle extract and hemp oil compared to the placebo emulgel, which was not the case with creams. Such findings indicated enhanced delivery of herbal active substances from emulgel compared to the cream.
T2  - Pharmaceutics
T1  - Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)
IS  - 11
VL  - 13
DO  - 10.3390/pharmaceutics13111919
ER  - 

@article{
author = "Tadic, Vanja M. and Zugic, Ana and Martinovic, Milica and Stankovic, Milica and Maksimović, Svetolik and Frank, Almut and Nesic, Ivana",
year = "2021",
abstract = "Immortelle, as rich source of chlorogenic acid and the phloroglucinol alpha-pyrone compound arzanol, possesses anti-inflammatory and antioxidant properties, affects cell regeneration, and has positive effect on many skin conditions. Hemp oil, characterized by a favorable omega-6 to omega-3 ratio, as well as an abundance of essential fatty acids and vitamin E, participates in immunoregulation and also act as an anti-inflammatory. In the present study, we examined the effect on the skin of creams and emulgels with immortelle extract and hemp oil, by comparing them to placebo samples and a non-treated control. A long-term in vivo study of biophysical skin characteristics, which lasted for 30 days, was conducted on 25 healthy human volunteers. Measured parameters were electrical capacitance of the stratum corneum, trans-epidermal water loss (TEWL), and skin pH and erythema index. Further, a sensory study was carried out in which the panelists had to choose descriptive terms for sensory attributes in questionnaire. The results showed that application of all preparations led to increase of skin hydration and TEWL reduction, while the skin was not irritated, and its normal pH was not disrupted. This study also showed importance of the carrier. Not only were emulgels described by panelists as preparations with better sensory properties, there was a significant difference between the skin hydration effect of emulgel with immortelle extract and hemp oil compared to the placebo emulgel, which was not the case with creams. Such findings indicated enhanced delivery of herbal active substances from emulgel compared to the cream.",
journal = "Pharmaceutics",
title = "Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)",
number = "11",
volume = "13",
doi = "10.3390/pharmaceutics13111919"
}

Tadic, V. M., Zugic, A., Martinovic, M., Stankovic, M., Maksimović, S., Frank, A.,& Nesic, I.. (2021). Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil). in Pharmaceutics, 13(11).
https://doi.org/10.3390/pharmaceutics13111919

Tadic VM, Zugic A, Martinovic M, Stankovic M, Maksimović S, Frank A, Nesic I. Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil). in Pharmaceutics. 2021;13(11).
doi:10.3390/pharmaceutics13111919 .

Tadic, Vanja M., Zugic, Ana, Martinovic, Milica, Stankovic, Milica, Maksimović, Svetolik, Frank, Almut, Nesic, Ivana, "Enhanced Skin Performance of Emulgel vs. Cream as Systems for Topical Delivery of Herbal Actives (Immortelle Extract and Hemp Oil)" in Pharmaceutics, 13, no. 11 (2021),
https://doi.org/10.3390/pharmaceutics13111919 . .

TY  - JOUR
AU  - Maksimović, Svetolik
AU  - Tadic, Vanja
AU  - Zvezdanovic, Jelena
AU  - Zizovic, Irena
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4904
AB  - Helichrysum italicum (Roth) G. Don fil., Asteraceae, possesses numerous secondary plant metabolites with a wide range of biological activities. Yet, data on the potential of supercritical fluid extraction (SFE) in their isolation are scarce. This study provides analyses of the chemical profiles of extracts obtained by SFE with or without ethanol as a cosolvent using GC-FID, GC-MS, HPLC, and UHPLC-MS techniques. Among the compounds with proven biological activity identified, the presence of arzanol was confirmed. In the next step, the integrated process of supercritical fluid extraction and impregnation was applied to deliver active compounds to cotton gauze and polypropylene fabric for possible topical applications. The analytical procedures results showed a considerable affinity of both textile materials for incorporating active components present in the H. italicum. The study indicated the high-pressure techniques applied as very efficient in the isolation of bioactive components from H. italicum and their adsorption on selected carriers.
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical CO2 in bioactive principles isolation from Helichrysum italicum and their adsorption on selected fabrics
VL  - 171
DO  - 10.1016/j.supflu.2021.105197
ER  - 

@article{
author = "Maksimović, Svetolik and Tadic, Vanja and Zvezdanovic, Jelena and Zizovic, Irena",
year = "2021",
abstract = "Helichrysum italicum (Roth) G. Don fil., Asteraceae, possesses numerous secondary plant metabolites with a wide range of biological activities. Yet, data on the potential of supercritical fluid extraction (SFE) in their isolation are scarce. This study provides analyses of the chemical profiles of extracts obtained by SFE with or without ethanol as a cosolvent using GC-FID, GC-MS, HPLC, and UHPLC-MS techniques. Among the compounds with proven biological activity identified, the presence of arzanol was confirmed. In the next step, the integrated process of supercritical fluid extraction and impregnation was applied to deliver active compounds to cotton gauze and polypropylene fabric for possible topical applications. The analytical procedures results showed a considerable affinity of both textile materials for incorporating active components present in the H. italicum. The study indicated the high-pressure techniques applied as very efficient in the isolation of bioactive components from H. italicum and their adsorption on selected carriers.",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical CO2 in bioactive principles isolation from Helichrysum italicum and their adsorption on selected fabrics",
volume = "171",
doi = "10.1016/j.supflu.2021.105197"
}

Maksimović, S., Tadic, V., Zvezdanovic, J.,& Zizovic, I.. (2021). Utilization of supercritical CO2 in bioactive principles isolation from Helichrysum italicum and their adsorption on selected fabrics. in Journal of Supercritical Fluids, 171.
https://doi.org/10.1016/j.supflu.2021.105197

Maksimović S, Tadic V, Zvezdanovic J, Zizovic I. Utilization of supercritical CO2 in bioactive principles isolation from Helichrysum italicum and their adsorption on selected fabrics. in Journal of Supercritical Fluids. 2021;171.
doi:10.1016/j.supflu.2021.105197 .

Maksimović, Svetolik, Tadic, Vanja, Zvezdanovic, Jelena, Zizovic, Irena, "Utilization of supercritical CO2 in bioactive principles isolation from Helichrysum italicum and their adsorption on selected fabrics" in Journal of Supercritical Fluids, 171 (2021),
https://doi.org/10.1016/j.supflu.2021.105197 . .