TY  - CONF
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Tyśkiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6894
AB  - The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals.
PB  - Valladolid : The lnstitute of Bioeconomy of University of Valladolid
C3  - Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain
T1  - The separation of biologically active extracts from chamomile seeds
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6894
ER  - 

@conference{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Tyśkiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "The main aim of this study was to evaluate chamomile seeds as a new source of valuable bioactive compounds. For this purpose, a green extraction technique was employed with supercritical carbon dioxide (sc-CO2) as a solvent and ethanol as a co-solvent. The extracts from chamomile seeds with an average particle size of 0.20 mm were extracted at pressures of 30 and 45 MPa and temperatures of 40 and 60 °C. The composition of extracts was investigated by a gas chromatography-mass spectrometry analytical method as well as Folin−Ciocalteu assay (for total phenolic content) and total flavonoid content test. The antioxidant activity of obtained chamomile extracts was evaluated using DPPH radical. The antimicrobial effect of extracts was tested on yeasts, Gram-positive, and Gram-negative bacteria while the cytotoxic effect was evaluated on normal and cancer cells. The extraction process parameters were evaluated and results showed a significant effect of operating pressure, temperature, and the addition of co-solvent on the amount of separated chamomile extract and its composition. The extraction yield ranged from 2.1 to 5.4% and extracts contained high amounts of unsaturated fatty acids. The addition of ethanol as a co-solvent (40 w/w%) had a slight influence on total phenolic and flavonoid contents increasing their values from 29.1 to 31.5 mg GAE/g and from 2.1 to 4.2 mg QE/g, respectively. The obtained supercritical chamomile extracts showed antioxidant activity achieving values of IC50 around 10 mg/mL. The antimicrobial activity of extracts was most pronounced against Bacillus subtilis. Extracts did not show cytotoxic activity against normal cells. Overall, results indicated a significant potential of extracts from chamomile seeds, produced in an environmentally friendly manner, to be used as a functional food or phytopharmaceuticals.",
publisher = "Valladolid : The lnstitute of Bioeconomy of University of Valladolid",
journal = "Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain",
title = "The separation of biologically active extracts from chamomile seeds",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6894"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Tyśkiewicz, K.,& Konkol, M.. (2023). The separation of biologically active extracts from chamomile seeds. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain
Valladolid : The lnstitute of Bioeconomy of University of Valladolid..
https://hdl.handle.net/21.15107/rcub_technorep_6894

Milovanović S, Grzegorczyk A, Świątek Ł, Tyśkiewicz K, Konkol M. The separation of biologically active extracts from chamomile seeds. in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6894 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Tyśkiewicz, Katarzyna, Konkol, Marcin, "The separation of biologically active extracts from chamomile seeds" in Book of abstracts / 2nd Greenering International Conference, 21–23th March 2023, Valladolid, Spain (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6894 .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Grzęda, Anita
AU  - Dębczak, Agnieszka
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6031
AB  - The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO2) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food.
PB  - Springer
T2  - Food and Bioprocess Technology
T1  - A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds
DO  - 10.1007/s11947-023-03038-9
ER  - 

@article{
author = "Milovanović, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Grzęda, Anita and Dębczak, Agnieszka and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2023",
abstract = "The main aim of this study was to evaluate the oils from chamomile seeds as a new source of bioactive compounds suitable for human consumption. A green extraction technique with supercritical carbon dioxide (sc-CO2) at pressures up to 450 bar and temperatures up to 60 °C was employed for the production of a high amount of biologically active oil. Additionally, exhausted waste material was re-extracted using sc-CO2 with the addition of ethanol. By optimization in operating pressure, temperature, production cost, fraction of milled seeds, and co-solvent addition, the amount of separated chamomile oil increased from 2.4 to 18.6% and the content of unsaturated fatty acids up to 88.7%. Oils contained α-bisabolol oxide A and B in amounts up to 1.4%. Linoleic acid was detected in an amount up to 711.1 mg/g and α-linolenic acid up to 27.5 mg/g. The total phenolic content in separated oil reached 80.4 mg GAE/g while the total flavonoid content reached 11.6 mg QE/g. The obtained chamomile oils showed antioxidant activity with an IC50 of up to 3.9 mg/mL. Among the 23 tested microorganisms, the antimicrobial activity of oils was the most pronounced against Gram-positive bacteria. The cytotoxic activity of oils was tested on normal and cancer-derived cell lines. Results indicated a significant potential for oil from chamomile seeds, produced in an eco-friendly manner, as a functional food.",
publisher = "Springer",
journal = "Food and Bioprocess Technology",
title = "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds",
doi = "10.1007/s11947-023-03038-9"
}

Milovanović, S., Grzegorczyk, A., Świątek, Ł., Grzęda, A., Dębczak, A., Tyskiewicz, K.,& Konkol, M.. (2023). A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds. in Food and Bioprocess Technology
Springer..
https://doi.org/10.1007/s11947-023-03038-9

Milovanović S, Grzegorczyk A, Świątek Ł, Grzęda A, Dębczak A, Tyskiewicz K, Konkol M. A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds. in Food and Bioprocess Technology. 2023;.
doi:10.1007/s11947-023-03038-9 .

Milovanović, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Grzęda, Anita, Dębczak, Agnieszka, Tyskiewicz, Katarzyna, Konkol, Marcin, "A Novel Strategy for the Separation of Functional Oils from Chamomile Seeds" in Food and Bioprocess Technology (2023),
https://doi.org/10.1007/s11947-023-03038-9 . .

TY  - JOUR
AU  - Milovanovic, Stoja
AU  - Grzegorczyk, Agnieszka
AU  - Świątek, Łukasz
AU  - Dębczak, Agnieszka
AU  - Tyskiewicz, Katarzyna
AU  - Konkol, Marcin
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5163
AB  - The present study was intended to explore dandelion seeds as a new source of biologically active constituents using an environmentally friendly extraction technique. The process-function relationships were determined by following the influence of temperature (40 and 60 °C) and pressure (from 100 to 450 bar) on the amount of separated extract, its density, composition, and bioactivity. It was shown that the supercritical CO2-assisted technique enables the separation of 0.6–25.0% extract having a density in a range of 827–941 kg/m3. Extracts contained a high amount of unsaturated fatty acids (89.9–90.6%) including linoleic acid (71%) and α-linolenic acid (up to 0.9%), as well as phenolic (5.5–12.1 mg GAE/g) and flavonoid compounds (208.6–564.5 μg QE/g). The antioxidant activity test showed that 20 mg/mL extract solutions inhibit 26.0–64.4% DPPH radical. In addition, extracts showed significant antimicrobial activity, especially against Gram-positive Bacillus subtilis and Micrococcus luteus. There was no cytotoxic effect on normal green monkey kidney cells and human skin fibroblasts nor on hypopharyngeal cancer, cervical adenocarcinoma, and colon cancer cells. The study indicated the possible safe application of bioactive dandelion seeds extract in the pharmaceutical, food, and cosmetic industry.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique
SP  - 100796
VL  - 29
DO  - 10.1016/j.scp.2022.100796
ER  - 

@article{
author = "Milovanovic, Stoja and Grzegorczyk, Agnieszka and Świątek, Łukasz and Dębczak, Agnieszka and Tyskiewicz, Katarzyna and Konkol, Marcin",
year = "2022",
abstract = "The present study was intended to explore dandelion seeds as a new source of biologically active constituents using an environmentally friendly extraction technique. The process-function relationships were determined by following the influence of temperature (40 and 60 °C) and pressure (from 100 to 450 bar) on the amount of separated extract, its density, composition, and bioactivity. It was shown that the supercritical CO2-assisted technique enables the separation of 0.6–25.0% extract having a density in a range of 827–941 kg/m3. Extracts contained a high amount of unsaturated fatty acids (89.9–90.6%) including linoleic acid (71%) and α-linolenic acid (up to 0.9%), as well as phenolic (5.5–12.1 mg GAE/g) and flavonoid compounds (208.6–564.5 μg QE/g). The antioxidant activity test showed that 20 mg/mL extract solutions inhibit 26.0–64.4% DPPH radical. In addition, extracts showed significant antimicrobial activity, especially against Gram-positive Bacillus subtilis and Micrococcus luteus. There was no cytotoxic effect on normal green monkey kidney cells and human skin fibroblasts nor on hypopharyngeal cancer, cervical adenocarcinoma, and colon cancer cells. The study indicated the possible safe application of bioactive dandelion seeds extract in the pharmaceutical, food, and cosmetic industry.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique",
pages = "100796",
volume = "29",
doi = "10.1016/j.scp.2022.100796"
}

Milovanovic, S., Grzegorczyk, A., Świątek, Ł., Dębczak, A., Tyskiewicz, K.,& Konkol, M.. (2022). Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 29, 100796.
https://doi.org/10.1016/j.scp.2022.100796

Milovanovic S, Grzegorczyk A, Świątek Ł, Dębczak A, Tyskiewicz K, Konkol M. Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique. in Sustainable Chemistry and Pharmacy. 2022;29:100796.
doi:10.1016/j.scp.2022.100796 .

Milovanovic, Stoja, Grzegorczyk, Agnieszka, Świątek, Łukasz, Dębczak, Agnieszka, Tyskiewicz, Katarzyna, Konkol, Marcin, "Dandelion seeds as a new and valuable source of bioactive extracts obtained using the supercritical fluid extraction technique" in Sustainable Chemistry and Pharmacy, 29 (2022):100796,
https://doi.org/10.1016/j.scp.2022.100796 . .