TY  - CONF
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Banjanac, Katarina
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Marinković, Aleksandar
AU  - Palomo, Jose Miguel
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6844
AB  - Flavonoids are a big class of plants’ secondary metabolites which possess antimicrobial, antioxidative, anti-inflammatory, etc. activities. However, their wider application is limited due to their poor solubility and stability in different environments. In order to overcome this problem and improve their biological and physicochemical characteristics, enzymatic oligomerization of flavonoids can be performed by using laccases as biocatalyst. In this study, laccase from Trametes versicolor was successfully immobilized onto two nanocarriers and used as a catalyst for the oligomerization of two flavonoids, esculin and phloridzin. For immobilization, nanocelulose prepared by ultrasonic treatment of commercial microcellulose and nanosilica from rice were used. Both carriers were modified in order to introduce amino groups on their surface: nanocelulose with poly(ethyleneimine) (NC-PEI) and nanosilica with organosylane (NS-OS). Most active preparation for NC-PEIlaccase and NS-OS-laccase showed activities of 10.05 IU/g of support and 9.08 IU/g of support, respectively. The oligomerization of flavonoids was performed using immobilized laccase and obtained results were compared with those obtained with free laccase (same amount of activity units) as biocatalyst. Immobilized preparations showed promising activities in reaction of esculin oligomerization, since after 24h of reaction the conversions of 73.3% and 61.6% with NC-PEI-laccase and NS-OS-laccase, respectively was achieved, while the soluble enzyme gave the conversion of 92.4%. On the other hand, some lower activity of NC-PEI-laccase was observed in reaction of phloridzin oligomerization, since the achieved conversion was 43.3% compared to 80.7% of phloridzin conversion obtained with soluble laccase. The lower activity could be explained by different reaction medium, since, compared to the esculin oligomerization, the phloridzin oligomerization was performed in 30% methanol, due to its low water solubility. However, obtained results indicate that novel immobilized laccase preparations can be successfully applied in the production of various flavonoid oligomers.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6844
ER  - 

@conference{
author = "Vukoičić, Ana and Milivojević, Ana and Banjanac, Katarina and Petrov Ivanković, Anja and Ćorović, Marija and Marinković, Aleksandar and Palomo, Jose Miguel and Bezbradica, Dejan",
year = "2023",
abstract = "Flavonoids are a big class of plants’ secondary metabolites which possess antimicrobial, antioxidative, anti-inflammatory, etc. activities. However, their wider application is limited due to their poor solubility and stability in different environments. In order to overcome this problem and improve their biological and physicochemical characteristics, enzymatic oligomerization of flavonoids can be performed by using laccases as biocatalyst. In this study, laccase from Trametes versicolor was successfully immobilized onto two nanocarriers and used as a catalyst for the oligomerization of two flavonoids, esculin and phloridzin. For immobilization, nanocelulose prepared by ultrasonic treatment of commercial microcellulose and nanosilica from rice were used. Both carriers were modified in order to introduce amino groups on their surface: nanocelulose with poly(ethyleneimine) (NC-PEI) and nanosilica with organosylane (NS-OS). Most active preparation for NC-PEIlaccase and NS-OS-laccase showed activities of 10.05 IU/g of support and 9.08 IU/g of support, respectively. The oligomerization of flavonoids was performed using immobilized laccase and obtained results were compared with those obtained with free laccase (same amount of activity units) as biocatalyst. Immobilized preparations showed promising activities in reaction of esculin oligomerization, since after 24h of reaction the conversions of 73.3% and 61.6% with NC-PEI-laccase and NS-OS-laccase, respectively was achieved, while the soluble enzyme gave the conversion of 92.4%. On the other hand, some lower activity of NC-PEI-laccase was observed in reaction of phloridzin oligomerization, since the achieved conversion was 43.3% compared to 80.7% of phloridzin conversion obtained with soluble laccase. The lower activity could be explained by different reaction medium, since, compared to the esculin oligomerization, the phloridzin oligomerization was performed in 30% methanol, due to its low water solubility. However, obtained results indicate that novel immobilized laccase preparations can be successfully applied in the production of various flavonoid oligomers.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6844"
}

Vukoičić, A., Milivojević, A., Banjanac, K., Petrov Ivanković, A., Ćorović, M., Marinković, A., Palomo, J. M.,& Bezbradica, D.. (2023). Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 27.
https://hdl.handle.net/21.15107/rcub_technorep_6844

Vukoičić A, Milivojević A, Banjanac K, Petrov Ivanković A, Ćorović M, Marinković A, Palomo JM, Bezbradica D. Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:27.
https://hdl.handle.net/21.15107/rcub_technorep_6844 .

Vukoičić, Ana, Milivojević, Ana, Banjanac, Katarina, Petrov Ivanković, Anja, Ćorović, Marija, Marinković, Aleksandar, Palomo, Jose Miguel, Bezbradica, Dejan, "Immobilization of laccase from Trametes versicolor on different nanocarriers and its application in the synthesis of flavonoid oligomers" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):27,
https://hdl.handle.net/21.15107/rcub_technorep_6844 .

TY  - JOUR
AU  - Salih, Rabab
AU  - Banjanac, Katarina
AU  - Vukoičić, Ana
AU  - Gržetić, Jelena
AU  - Popović, Ana
AU  - Veljković, Milica
AU  - Bezbradica, Dejan
AU  - Marinković, Aleksandar
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5278
AB  - The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles).
PB  - Elsevier Ltd.
T2  - Journal of Environmental Chemical Engineering
T1  - Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes
IS  - 1
SP  - 109077
VL  - 11
DO  - 10.1016/j.jece.2022.109077
ER  - 

@article{
author = "Salih, Rabab and Banjanac, Katarina and Vukoičić, Ana and Gržetić, Jelena and Popović, Ana and Veljković, Milica and Bezbradica, Dejan and Marinković, Aleksandar",
year = "2023",
abstract = "The novel lignin-based microspheres (LMS-DEGDMA) were produced through a two-step modification procedure consisting of kraft lignin (KL) modification with acrylic acid synthesized from fructose; and suspension and copolymerization of acrylic acid modified KL (KL-A) with diethylene glycol dimethacrylate (DEGDMA). The applied procedure provided LMS-DEGDMA microspheres in the size range of 50-90 μm with specific surface area of 39 m2/g, porosity ϵp 62, pore diameters of 4-20 nm and total pore volume of 0.14 cm3/g. Immobilization of laccase from Myceliophthora thermophilia expressed in Aspergillus oryzae (Novozyme® 51003) on LMS-DEGDMA was explored. By optimization of the immobilization process, it was demonstrated that laccase immobilization on LMS-DEGDMA exhibited the best results (protein immobilization yield of 70 %, activity yield of 27 %, and catalytic activity of 262 IU/g of support) when the immobilization was carried out at pH 5.0 and 100 mg of offered protein per g support during 1 h. Additionally, the LMS-DEGDMA-laccase preparation showed efficiency (>80 %, 5-7 cycles) in decolorization of anthraquinone dyes (Lanaset® violet B, Lanaset® blue 2 R and C.I. Acid Green 40) and their mixture. In case of Lanaset® violet B, LMS-DEGDMA improved laccase's kinetics characteristics (90 % over 24 h) along with operational stability (> 63 % catalytic activity, 7 cycles).",
publisher = "Elsevier Ltd.",
journal = "Journal of Environmental Chemical Engineering",
title = "Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes",
number = "1",
pages = "109077",
volume = "11",
doi = "10.1016/j.jece.2022.109077"
}

Salih, R., Banjanac, K., Vukoičić, A., Gržetić, J., Popović, A., Veljković, M., Bezbradica, D.,& Marinković, A.. (2023). Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. in Journal of Environmental Chemical Engineering
Elsevier Ltd.., 11(1), 109077.
https://doi.org/10.1016/j.jece.2022.109077

Salih R, Banjanac K, Vukoičić A, Gržetić J, Popović A, Veljković M, Bezbradica D, Marinković A. Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes. in Journal of Environmental Chemical Engineering. 2023;11(1):109077.
doi:10.1016/j.jece.2022.109077 .

Salih, Rabab, Banjanac, Katarina, Vukoičić, Ana, Gržetić, Jelena, Popović, Ana, Veljković, Milica, Bezbradica, Dejan, Marinković, Aleksandar, "Acrylic modified kraft lignin microspheres as novel support for immobilization of laccase from M.Thermophila expressed in A. oryzae (Novozym® 51003) and application in degradation of anthraquinone textile dyes" in Journal of Environmental Chemical Engineering, 11, no. 1 (2023):109077,
https://doi.org/10.1016/j.jece.2022.109077 . .

TY  - JOUR
AU  - Petrov Ivanković, Anja
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Jansen, Patrick
AU  - Vukoičić, Ana
AU  - van den Bogaard, Ellen
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6870
AB  - Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Chemical and Biological Technologies in Agriculture
T1  - In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives
IS  - 1
SP  - 125
VL  - 10
DO  - 10.1186/s40538-023-00502-8
ER  - 

@article{
author = "Petrov Ivanković, Anja and Milivojević, Ana and Ćorović, Marija and Simović, Milica and Banjanac, Katarina and Jansen, Patrick and Vukoičić, Ana and van den Bogaard, Ellen and Bezbradica, Dejan",
year = "2023",
abstract = "Background Blackcurrant is a rich source of polyphenols with proven physiological activity, that have lately been classified as emerging prebiotics, making its extracts suitable for application in functional food and bioactive cosmetics. However, these biomolecules are trapped in cell wall structures and nowadays, enzymatic hydrolysis is recognised as powerful tool for extraction efficiency improvement in an environmentally sound manner. This study aimed to optimize enzyme-assisted extraction to maximize yields of blackcurrant polyphenols. In addition, their prebiotic potential was tested by examining their influence on typical skin microbiota representatives as model microorganisms—beneficial coagulase-negative Staphylococcus epidermidis and two opportunistic pathogens, Staphylococcus aureus and Cutibacterium acnes. Results Among four examined commercial enzyme preparations, Viscozyme® L and Rohapect® MC, demonstrated the highest potential as extraction enhancers by increasing antioxidant activity, total polyphenol, and flavonoid contents. Furthermore, their synergetic effect enabled 95.3% increase in polyphenol concentration compared to conventional extraction with a maximum of 27.6 mg gallic acid equivalents (GAE)/g dry matter (DM) at a total enzyme concentration of 0.05 mL/g DM (Viscozyme® L to Rohapect® MC ratio 2:1) after 60 min. HPLC profiles of two samples showed differences in polyphenol composition indicating simultaneous extraction–biotransformation. At a concentration of 0.025 mg GAE/mL, PAS (prebiotic activity score) values were 0.096 and 0.172 for conventionally and enzymatically obtained extract, respectively. Moderate inhibitory effect of optimal extract concentration on Cutibacterium acnes was shown, as well. Hydrogel prepared with optimal extract concentration showed improved hydration effect, decreased irritability and increased dermatological compatibility comparing to basic formulation. Conclusions Overall results demonstrated that blackcurrant extract obtained under optimized conditions using enzymes possesses an emerging prebiotic potential for selective skin microbiota stimulation.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Chemical and Biological Technologies in Agriculture",
title = "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives",
number = "1",
pages = "125",
volume = "10",
doi = "10.1186/s40538-023-00502-8"
}

Petrov Ivanković, A., Milivojević, A., Ćorović, M., Simović, M., Banjanac, K., Jansen, P., Vukoičić, A., van den Bogaard, E.,& Bezbradica, D.. (2023). In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture
Springer Science and Business Media Deutschland GmbH., 10(1), 125.
https://doi.org/10.1186/s40538-023-00502-8

Petrov Ivanković A, Milivojević A, Ćorović M, Simović M, Banjanac K, Jansen P, Vukoičić A, van den Bogaard E, Bezbradica D. In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives. in Chemical and Biological Technologies in Agriculture. 2023;10(1):125.
doi:10.1186/s40538-023-00502-8 .

Petrov Ivanković, Anja, Milivojević, Ana, Ćorović, Marija, Simović, Milica, Banjanac, Katarina, Jansen, Patrick, Vukoičić, Ana, van den Bogaard, Ellen, Bezbradica, Dejan, "In vitro evaluation of enzymatically derived blackcurrant extract as prebiotic cosmetic ingredient: extraction conditions optimization and effect on cutaneous microbiota representatives" in Chemical and Biological Technologies in Agriculture, 10, no. 1 (2023):125,
https://doi.org/10.1186/s40538-023-00502-8 . .

TY  - CONF
AU  - Ortega-Nieto, Clara
AU  - Losada-Gracia, Noelia
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Bezbradica, Dejan
AU  - Palomo, Jose M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6959
AB  - In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6959
ER  - 

@conference{
author = "Ortega-Nieto, Clara and Losada-Gracia, Noelia and Vukoičić, Ana and Milivojević, Ana and Bezbradica, Dejan and Palomo, Jose M.",
year = "2023",
abstract = "In recent years, the synthesis of novel artificial metalloenzymes by the combination of
metal or complex organometallic systems and enzymes is rapidly growing field of research in
catalytic applications. In this study, new artificial metalloenzymes have been designed and
developed based on the combination of an enzyme and different metal salts. For this purpose,
novel synthesis method has been used, where the enzyme, Lipase B from Candida antarctica
(CALB), acts as an inducer of metal nanoparticles of copper and silver. Obtained biohybrids
show improved stability properties compared to natural enzymes. Moreover, the synergetic
effect between copper and silver has been evaluated, looking for an improvement in the
catalytic efficiency of an only copper-metalloenzyme previously developed.
The copper-silver-CALB metalloenzymes have been characterized by X-Ray
diffraction analysis, mass spectrometry, and SEM and TEM as microscopies techniques. After
that, their catalytic efficiency was tested in different reactions, in particular in the phloridzin
oligomerization as laccase-like activity.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6959"
}

Ortega-Nieto, C., Losada-Gracia, N., Vukoičić, A., Milivojević, A., Bezbradica, D.,& Palomo, J. M.. (2023). NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 39.
https://hdl.handle.net/21.15107/rcub_technorep_6959

Ortega-Nieto C, Losada-Gracia N, Vukoičić A, Milivojević A, Bezbradica D, Palomo JM. NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:39.
https://hdl.handle.net/21.15107/rcub_technorep_6959 .

Ortega-Nieto, Clara, Losada-Gracia, Noelia, Vukoičić, Ana, Milivojević, Ana, Bezbradica, Dejan, Palomo, Jose M., "NOVEL Cu/Ag-ENZYME BIOHYBRIDS AS ARTIFICIAL METALLOENZYME" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):39,
https://hdl.handle.net/21.15107/rcub_technorep_6959 .

TY  - CONF
AU  - García-Sanz, Carla
AU  - Andreu, Alicia
AU  - de las Rivas, Blanca
AU  - Muñoz, Rosario
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Bezbradica, Dejan
AU  - Palomo, Jose Miguel
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6949
AB  - Laccase (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductase) is a blue copper oxidase
of great industrial interest due to its ability to catalyseoxidation processes of phenols and
persistent organic pollutants. However, it is susceptible to denaturation by high temperatures,
and sensitive to pH and the presence of high concentrations of solvents, which is a problem
for industrial use. To solve this problem, this project develops the synthesis in aqueous
medium of a new Mn metalloenzyme with laccase oxidase mimetic catalytic activity. To do
this, Geobacillus thermocatenulatus lipase (GTL) is used as a "scaffold" enzyme, which is
mixed with a manganese salt at 50 ºC in an aqueous medium. In this way, the in situ
formation of manganese (IV) oxide nanowires is generated, interacting with the enzyme and
obtaining the GTL-Mn bioconjugate. On the other hand, its oxidative activity was evaluated
using the ABTS assay, obtaining a specificity 300 times greater than the laccase from
Trametes versicolor and 2 times more than the laccase from Myceliophthora thermophila
expressed in Aspergillus oryzae (Novozym 51003®). In addition, the new metalloenzyme
turned out to be 2 times more stable at 40 ºC, 3 times more stable in thepresence of 10% can,
and 10 times more stable at 20% and 30% AcN than laccase (Novozym 51003®) by
evaluating it at 2 hour incubation. Moreover, it was shown that the use of immobilization
strategies improves the stabilization of this artificial metalloenzyme two times more. Finally,
the novel Mn metalloenzymes were effective in the reaction of phlorizin oligomerization.
Future research will aim to extend this study for the use of flavonoids as prebiotic molecules
in the cosmetics industry.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6949
ER  - 

@conference{
author = "García-Sanz, Carla and Andreu, Alicia and de las Rivas, Blanca and Muñoz, Rosario and Vukoičić, Ana and Milivojević, Ana and Bezbradica, Dejan and Palomo, Jose Miguel",
year = "2023",
abstract = "Laccase (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductase) is a blue copper oxidase
of great industrial interest due to its ability to catalyseoxidation processes of phenols and
persistent organic pollutants. However, it is susceptible to denaturation by high temperatures,
and sensitive to pH and the presence of high concentrations of solvents, which is a problem
for industrial use. To solve this problem, this project develops the synthesis in aqueous
medium of a new Mn metalloenzyme with laccase oxidase mimetic catalytic activity. To do
this, Geobacillus thermocatenulatus lipase (GTL) is used as a "scaffold" enzyme, which is
mixed with a manganese salt at 50 ºC in an aqueous medium. In this way, the in situ
formation of manganese (IV) oxide nanowires is generated, interacting with the enzyme and
obtaining the GTL-Mn bioconjugate. On the other hand, its oxidative activity was evaluated
using the ABTS assay, obtaining a specificity 300 times greater than the laccase from
Trametes versicolor and 2 times more than the laccase from Myceliophthora thermophila
expressed in Aspergillus oryzae (Novozym 51003®). In addition, the new metalloenzyme
turned out to be 2 times more stable at 40 ºC, 3 times more stable in thepresence of 10% can,
and 10 times more stable at 20% and 30% AcN than laccase (Novozym 51003®) by
evaluating it at 2 hour incubation. Moreover, it was shown that the use of immobilization
strategies improves the stabilization of this artificial metalloenzyme two times more. Finally,
the novel Mn metalloenzymes were effective in the reaction of phlorizin oligomerization.
Future research will aim to extend this study for the use of flavonoids as prebiotic molecules
in the cosmetics industry.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6949"
}

García-Sanz, C., Andreu, A., de las Rivas, B., Muñoz, R., Vukoičić, A., Milivojević, A., Bezbradica, D.,& Palomo, J. M.. (2023). STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 35.
https://hdl.handle.net/21.15107/rcub_technorep_6949

García-Sanz C, Andreu A, de las Rivas B, Muñoz R, Vukoičić A, Milivojević A, Bezbradica D, Palomo JM. STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:35.
https://hdl.handle.net/21.15107/rcub_technorep_6949 .

García-Sanz, Carla, Andreu, Alicia, de las Rivas, Blanca, Muñoz, Rosario, Vukoičić, Ana, Milivojević, Ana, Bezbradica, Dejan, Palomo, Jose Miguel, "STUDY AND PREPARATION OF ARTIFICIAL MANGANESE METALLOENZYMES WITH LACCASE LIKE ACTIVITY" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):35,
https://hdl.handle.net/21.15107/rcub_technorep_6949 .

TY  - CONF
AU  - Vasić, Katja
AU  - Primožić, Mateja
AU  - Leitgeb, Maja
AU  - Knez, Željko
AU  - Vukoičić, Ana
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6960
AB  - In this work, immobilization of laccase from Trametes versicolor using different
immobilization techniques was investigated and obtained results regarding immobilized
enzyme activity were compared. The enzyme was successfully immobilized onto different
nanoparticles such as magnetic (MNPs) as well as cellulose (CNP) and silica nanoparticles
(SNP). Functionalized MNPs were prepared by co-precipitation of Fe2+ and Fe3+ ions,
which were initially coated with a layer of citric acid to prevent particle agglomeration.
Further, they were coated with sodium silicate and functionalized with aminosilane. MNPs
were activated with the crosslinking reagent glutaraldehyde and laccase was immobilized
onto functionalized MNPs. On the other hand, CNPs were prepared by ultrasonic treatment of
microcellulose while the SNPs were obtained from rice. Functionalization of these
nanoparticles was performed by introducing amino groups onto their surface, by modification
of CNPs with poly(ethyleneimine) and with organosilane in case of SNPs. Additionally, the
immobilized laccase in the form of cross-linked enzyme aggregates (Lac-CLEAs) was
prepared by simple immobilization method involving precipitation of the enzyme from
aqueous buffer using ethanol as precipitation solvent followed by cross-linking of aggregates
of enzyme molecules by glutaraldehyde. When the magnetic cross-linked enzyme aggregates
from laccase (Lac-mCLEAs) were synthesized, magnetic nanoparticles were added to the
synthesis process. The immobilization yield of laccase immobilized onto nanoparticles was
95%, 83% and 84% for MNPs, CNPs and SNPs, respectively, followed by activity yield of
77%, 73% and 66%, respectively. Some lower immobilization yield was achieved for the
Lac-CLEAs and Lac-mCLEAs, 74% and 75%, respectively. Lac-mCLEAs showed the
highest activity yield (85%) while activity yield of 76% was achieved for Lac-CLEAs when
BSA, as proteic feeder was used. Obtained results suggest that laccase could be successfully
immobilized using different supports and immobilization techniques.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - INFLUENCE OF DIFFERENT IMMOBILIZATION TECHNIQUES ON IMMOBILIZED LACCASE ACTIVITY
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6960
ER  - 

@conference{
author = "Vasić, Katja and Primožić, Mateja and Leitgeb, Maja and Knez, Željko and Vukoičić, Ana and Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana",
year = "2023",
abstract = "In this work, immobilization of laccase from Trametes versicolor using different
immobilization techniques was investigated and obtained results regarding immobilized
enzyme activity were compared. The enzyme was successfully immobilized onto different
nanoparticles such as magnetic (MNPs) as well as cellulose (CNP) and silica nanoparticles
(SNP). Functionalized MNPs were prepared by co-precipitation of Fe2+ and Fe3+ ions,
which were initially coated with a layer of citric acid to prevent particle agglomeration.
Further, they were coated with sodium silicate and functionalized with aminosilane. MNPs
were activated with the crosslinking reagent glutaraldehyde and laccase was immobilized
onto functionalized MNPs. On the other hand, CNPs were prepared by ultrasonic treatment of
microcellulose while the SNPs were obtained from rice. Functionalization of these
nanoparticles was performed by introducing amino groups onto their surface, by modification
of CNPs with poly(ethyleneimine) and with organosilane in case of SNPs. Additionally, the
immobilized laccase in the form of cross-linked enzyme aggregates (Lac-CLEAs) was
prepared by simple immobilization method involving precipitation of the enzyme from
aqueous buffer using ethanol as precipitation solvent followed by cross-linking of aggregates
of enzyme molecules by glutaraldehyde. When the magnetic cross-linked enzyme aggregates
from laccase (Lac-mCLEAs) were synthesized, magnetic nanoparticles were added to the
synthesis process. The immobilization yield of laccase immobilized onto nanoparticles was
95%, 83% and 84% for MNPs, CNPs and SNPs, respectively, followed by activity yield of
77%, 73% and 66%, respectively. Some lower immobilization yield was achieved for the
Lac-CLEAs and Lac-mCLEAs, 74% and 75%, respectively. Lac-mCLEAs showed the
highest activity yield (85%) while activity yield of 76% was achieved for Lac-CLEAs when
BSA, as proteic feeder was used. Obtained results suggest that laccase could be successfully
immobilized using different supports and immobilization techniques.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "INFLUENCE OF DIFFERENT IMMOBILIZATION TECHNIQUES ON IMMOBILIZED LACCASE ACTIVITY",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6960"
}

Vasić, K., Primožić, M., Leitgeb, M., Knez, Ž., Vukoičić, A., Petrov Ivanković, A., Ćorović, M.,& Milivojević, A.. (2023). INFLUENCE OF DIFFERENT IMMOBILIZATION TECHNIQUES ON IMMOBILIZED LACCASE ACTIVITY. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 40.
https://hdl.handle.net/21.15107/rcub_technorep_6960

Vasić K, Primožić M, Leitgeb M, Knez Ž, Vukoičić A, Petrov Ivanković A, Ćorović M, Milivojević A. INFLUENCE OF DIFFERENT IMMOBILIZATION TECHNIQUES ON IMMOBILIZED LACCASE ACTIVITY. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:40.
https://hdl.handle.net/21.15107/rcub_technorep_6960 .

Vasić, Katja, Primožić, Mateja, Leitgeb, Maja, Knez, Željko, Vukoičić, Ana, Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, "INFLUENCE OF DIFFERENT IMMOBILIZATION TECHNIQUES ON IMMOBILIZED LACCASE ACTIVITY" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):40,
https://hdl.handle.net/21.15107/rcub_technorep_6960 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Banjanac, Katarina
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Vukoičić, Ana
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6843
AB  - With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.
PB  - Belgrade : University, Faculty of Geography
C3  - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
T1  - Effective approach for obtaining high-purity prebiotics using membrane separation technology
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6843
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Vukoičić, Ana and Bezbradica, Dejan",
year = "2023",
abstract = "With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.",
publisher = "Belgrade : University, Faculty of Geography",
journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023",
title = "Effective approach for obtaining high-purity prebiotics using membrane separation technology",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6843"
}

Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Vukoičić, A.,& Bezbradica, D.. (2023). Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023
Belgrade : University, Faculty of Geography., 25.
https://hdl.handle.net/21.15107/rcub_technorep_6843

Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Vukoičić A, Bezbradica D. Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:25.
https://hdl.handle.net/21.15107/rcub_technorep_6843 .

Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Vukoičić, Ana, Bezbradica, Dejan, "Effective approach for obtaining high-purity prebiotics using membrane separation technology" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):25,
https://hdl.handle.net/21.15107/rcub_technorep_6843 .

TY  - CONF
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Baščarević, Jana
AU  - Petrov Ivanković, Anja
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6945
AB  - Dihydrochalcones are a class of flavonoids found in apple trees (Malus sp) generally
present in high levels in young leaves and immature fruits. Phloridzin is a member of the
dihydrochalcones which possess good antioxidant activity, as well as a protective role against
UV radiation, hence it is a constituent of various commercial cosmetic preparations. These
natural low-molecular weight phenolic compounds with diverse physicochemical
characteristics are substrates for laccases, copper-containing polyphenol oxidases whose
activity can yield compounds that vary in structure and biological activities. The positive
effect of laccase-catalyzed polymerization of flavonoids on the functionality of produced
oligomers or polymers has been already shown on some flavonoids’ representatives.
In this work, fungal laccase from Trametes versicolor was applied as a biocatalyst in
the reaction of phloridzin oligomerization. Synthesized phloridzin oligomers were separated
from the reaction mixture and lyophilized in order to test their antioxidant and antimicrobial
activity. Different antioxidant assays, such as ABTS (2,2’-azino-bis(3-
ethylbenzenothiazoline-6-sulfonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-
picrylhydrazyl) showed that in comparison with the monomer, synthesized oligomers had the
weaker ability to scavenge the free ABTS•+ and DPPH•
radicals, since for both assays,
examined IC50 values of phloridzin oligomers (0.46 mg/ml and 1.52 mg/ml, respectively)
was higher than the values for parent molecule (0.13 mg/ml and 1.07 mg/ml, respectively).
This could be ascribed to the fact that some of the functional groups responsible for the
antioxidant activity probably participate in the formation of linkage between phloridzin units
in the structure of oligomers. On the other hand, synthesized phloridzin oligomers
demonstrated a significantly higher antimicrobial effect on the Gram-positive
bacteria Staphylococcus aureus compared to the parent molecule phloridzin. Moreover, the
greater inhibition of bacterial growth was achieved by increasing the oligomers’
concentration. Given the obtained results, synthesized compounds show potential to be
further examined as dermal prebiotics.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6945
ER  - 

@conference{
author = "Vukoičić, Ana and Milivojević, Ana and Baščarević, Jana and Petrov Ivanković, Anja and Banjanac, Katarina and Ćorović, Marija and Bezbradica, Dejan",
year = "2023",
abstract = "Dihydrochalcones are a class of flavonoids found in apple trees (Malus sp) generally
present in high levels in young leaves and immature fruits. Phloridzin is a member of the
dihydrochalcones which possess good antioxidant activity, as well as a protective role against
UV radiation, hence it is a constituent of various commercial cosmetic preparations. These
natural low-molecular weight phenolic compounds with diverse physicochemical
characteristics are substrates for laccases, copper-containing polyphenol oxidases whose
activity can yield compounds that vary in structure and biological activities. The positive
effect of laccase-catalyzed polymerization of flavonoids on the functionality of produced
oligomers or polymers has been already shown on some flavonoids’ representatives.
In this work, fungal laccase from Trametes versicolor was applied as a biocatalyst in
the reaction of phloridzin oligomerization. Synthesized phloridzin oligomers were separated
from the reaction mixture and lyophilized in order to test their antioxidant and antimicrobial
activity. Different antioxidant assays, such as ABTS (2,2’-azino-bis(3-
ethylbenzenothiazoline-6-sulfonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-
picrylhydrazyl) showed that in comparison with the monomer, synthesized oligomers had the
weaker ability to scavenge the free ABTS•+ and DPPH•
radicals, since for both assays,
examined IC50 values of phloridzin oligomers (0.46 mg/ml and 1.52 mg/ml, respectively)
was higher than the values for parent molecule (0.13 mg/ml and 1.07 mg/ml, respectively).
This could be ascribed to the fact that some of the functional groups responsible for the
antioxidant activity probably participate in the formation of linkage between phloridzin units
in the structure of oligomers. On the other hand, synthesized phloridzin oligomers
demonstrated a significantly higher antimicrobial effect on the Gram-positive
bacteria Staphylococcus aureus compared to the parent molecule phloridzin. Moreover, the
greater inhibition of bacterial growth was achieved by increasing the oligomers’
concentration. Given the obtained results, synthesized compounds show potential to be
further examined as dermal prebiotics.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6945"
}

Vukoičić, A., Milivojević, A., Baščarević, J., Petrov Ivanković, A., Banjanac, K., Ćorović, M.,& Bezbradica, D.. (2023). EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 26.
https://hdl.handle.net/21.15107/rcub_technorep_6945

Vukoičić A, Milivojević A, Baščarević J, Petrov Ivanković A, Banjanac K, Ćorović M, Bezbradica D. EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:26.
https://hdl.handle.net/21.15107/rcub_technorep_6945 .

Vukoičić, Ana, Milivojević, Ana, Baščarević, Jana, Petrov Ivanković, Anja, Banjanac, Katarina, Ćorović, Marija, Bezbradica, Dejan, "EVALUATION OF BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED PHLORIDZIN OLIGOMERS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):26,
https://hdl.handle.net/21.15107/rcub_technorep_6945 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Veljković, Milica
AU  - Vukoičić, Ana
AU  - Simović, Milica
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6946
AB  - Berries are known to be one of the richest sources of polyphenols which can offer
various health benefits. Dietary supplementation with berries has a positive influence on the
gut microbiota, which directly affects overall health, including skin health. However, topical
application of berry polyphenols has been used mainly for its antioxidant activity to prevent
premature aging and improve the skin's appearance. Therefore, this study examined the
content of different polyphenol classes of cranberry (Vaccinium macrocarpa), chokeberry
(Aronia melanocarpa) and blueberry (Vaccinium angustifolium) extracts, investigating their
antioxidant properties and potential impact on skin as topical prebiotics. The prebiotic
capacity of these extracts in applied concentrations range of 0.015-0.05 mg GAE/mL, was
determined against two cutaneous bacteria - beneficial Staphylococcus epidermidis and
opportunistic pathogen Staphylococcus aureus, since the disrupted balance between them
may contribute worsening of atopic dermatitis. The obtained results showed that the total
polyphenol content was highest in chokeberry extract (9.88 mg GAE/g DM), followed by
cranberry extract (8.78 mg GAE/g DM), and the lowest in blueberry extract (6.48 mg GAE/g
DM). Chokeberry extract was also richest in flavonoids, flavonols, anthocyanins and phenolic
acids. Notably, cranberry extract had the highest concentration of tannins, almost three times
higher compared to blueberry extract. According to DPPH and ABTS methods, the
antioxidant activity was significantly high in chokeberry extract, while FRAP method
revealed that cranberry extract is the most potent antioxidant. Regarding prebiotic capacity,
positive values (0.10-0.48) were observed at all concentrations of cranberry extract, with a
trend indicating a decrease in prebiotic capacity as polyphenol concentration increases.
Prebiotic capacities of blueberry and chokeberry extracts had either negative values or values
equal to zero, indicating that these extracts do not positively impact the microorganisms’
ratio. Obtained findings suggest cranberry extract potential for enhancing both antioxidant
defense of skin and rebalancing cutaneous microbiota.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6946
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Veljković, Milica and Vukoičić, Ana and Simović, Milica and Bezbradica, Dejan",
year = "2023",
abstract = "Berries are known to be one of the richest sources of polyphenols which can offer
various health benefits. Dietary supplementation with berries has a positive influence on the
gut microbiota, which directly affects overall health, including skin health. However, topical
application of berry polyphenols has been used mainly for its antioxidant activity to prevent
premature aging and improve the skin's appearance. Therefore, this study examined the
content of different polyphenol classes of cranberry (Vaccinium macrocarpa), chokeberry
(Aronia melanocarpa) and blueberry (Vaccinium angustifolium) extracts, investigating their
antioxidant properties and potential impact on skin as topical prebiotics. The prebiotic
capacity of these extracts in applied concentrations range of 0.015-0.05 mg GAE/mL, was
determined against two cutaneous bacteria - beneficial Staphylococcus epidermidis and
opportunistic pathogen Staphylococcus aureus, since the disrupted balance between them
may contribute worsening of atopic dermatitis. The obtained results showed that the total
polyphenol content was highest in chokeberry extract (9.88 mg GAE/g DM), followed by
cranberry extract (8.78 mg GAE/g DM), and the lowest in blueberry extract (6.48 mg GAE/g
DM). Chokeberry extract was also richest in flavonoids, flavonols, anthocyanins and phenolic
acids. Notably, cranberry extract had the highest concentration of tannins, almost three times
higher compared to blueberry extract. According to DPPH and ABTS methods, the
antioxidant activity was significantly high in chokeberry extract, while FRAP method
revealed that cranberry extract is the most potent antioxidant. Regarding prebiotic capacity,
positive values (0.10-0.48) were observed at all concentrations of cranberry extract, with a
trend indicating a decrease in prebiotic capacity as polyphenol concentration increases.
Prebiotic capacities of blueberry and chokeberry extracts had either negative values or values
equal to zero, indicating that these extracts do not positively impact the microorganisms’
ratio. Obtained findings suggest cranberry extract potential for enhancing both antioxidant
defense of skin and rebalancing cutaneous microbiota.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS",
pages = "29",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6946"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Veljković, M., Vukoičić, A., Simović, M.,& Bezbradica, D.. (2023). DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 29.
https://hdl.handle.net/21.15107/rcub_technorep_6946

Petrov Ivanković A, Ćorović M, Milivojević A, Veljković M, Vukoičić A, Simović M, Bezbradica D. DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:29.
https://hdl.handle.net/21.15107/rcub_technorep_6946 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Veljković, Milica, Vukoičić, Ana, Simović, Milica, Bezbradica, Dejan, "DISCOVERING POTENTIAL OF POLYPHENOL COMPOUNDS FROM BLUEBERRY, CRANBERRY AND CHOKEBERRY EXTRACTS AS SKIN PREBIOTICS" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):29,
https://hdl.handle.net/21.15107/rcub_technorep_6946 .

TY  - CONF
AU  - Veljković, Milica
AU  - Simović, Milica
AU  - Petrov Ivanković, Anja
AU  - Vukoičić, Ana
AU  - Banjanac, Katarina
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5993
AB  - It is well known that maple syrup, a product obtained by processing maple tree sap, is widely
used in many households due to its sweetness and beneficial ingredients. It is most often used
as a topping for pancakes, waffles, donuts, and other desserts, but also as a sweetener for
numerous beverages such as coffee, tea, lemonade, and many others. This natural syrup, in
which, in addition to numerous minerals, antioxidants, carbohydrates and other components,
sucrose predominates, presents an excellent substrate for the synthesis of bioactive
molecules, i.e. fructo-oligosaccharides (FOS). These indigestible oligosaccharides which
belong to a group of established prebiotics are very suitable from the standpoint of human
health because they enable the normal functioning of the gastrointestinal tract, have a
positive effect on the immune system as well as many other benefits for the human organism.
With the approach which include the conversion of sucrose into FOS, it is possible to obtain a
product with improved characteristics, i.e. higher functional and lower caloric values.
Accordingly, in this study, a detailed optimization of the enzymatic synthesis of FOS was
performed using maple syrup as a source of sucrose and commercial enzyme mixture
Pectinex® Ultra SP-L as a source of fructosyltransferase. Namely, by individual varying of
enzymatic synthesis key factors such as temperature (30-80 °C), enzyme concentration (1, 3
and 5%) and reaction time (0-25 h), optimal conditions were selected. It was determined that
by performing the synthesis reaction at a temperature of 60 °C with an enzyme concentration
of 3% for 12h, a remarkable sucrose hydrolysis degree of 82% and a FOS yield of approximately 55% were achieved. In this case, the estimated caloric value of the obtained
product is about 2.2 kcal/mL, which is around 1.6-fold lower compared to the initial value of
3.6 kcal/mL. The product obtained in this manner could represent a low-calorie sweetener
with high fiber content and could potentially be included in the diet of diabetics and obese
people, as well as all those who aim to maintain better overall health
PB  - Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia
C3  - 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
T1  - In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product
EP  - 153
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5993
ER  - 

@conference{
author = "Veljković, Milica and Simović, Milica and Petrov Ivanković, Anja and Vukoičić, Ana and Banjanac, Katarina and Bezbradica, Dejan",
year = "2022",
abstract = "It is well known that maple syrup, a product obtained by processing maple tree sap, is widely
used in many households due to its sweetness and beneficial ingredients. It is most often used
as a topping for pancakes, waffles, donuts, and other desserts, but also as a sweetener for
numerous beverages such as coffee, tea, lemonade, and many others. This natural syrup, in
which, in addition to numerous minerals, antioxidants, carbohydrates and other components,
sucrose predominates, presents an excellent substrate for the synthesis of bioactive
molecules, i.e. fructo-oligosaccharides (FOS). These indigestible oligosaccharides which
belong to a group of established prebiotics are very suitable from the standpoint of human
health because they enable the normal functioning of the gastrointestinal tract, have a
positive effect on the immune system as well as many other benefits for the human organism.
With the approach which include the conversion of sucrose into FOS, it is possible to obtain a
product with improved characteristics, i.e. higher functional and lower caloric values.
Accordingly, in this study, a detailed optimization of the enzymatic synthesis of FOS was
performed using maple syrup as a source of sucrose and commercial enzyme mixture
Pectinex® Ultra SP-L as a source of fructosyltransferase. Namely, by individual varying of
enzymatic synthesis key factors such as temperature (30-80 °C), enzyme concentration (1, 3
and 5%) and reaction time (0-25 h), optimal conditions were selected. It was determined that
by performing the synthesis reaction at a temperature of 60 °C with an enzyme concentration
of 3% for 12h, a remarkable sucrose hydrolysis degree of 82% and a FOS yield of approximately 55% were achieved. In this case, the estimated caloric value of the obtained
product is about 2.2 kcal/mL, which is around 1.6-fold lower compared to the initial value of
3.6 kcal/mL. The product obtained in this manner could represent a low-calorie sweetener
with high fiber content and could potentially be included in the diet of diabetics and obese
people, as well as all those who aim to maintain better overall health",
publisher = "Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia",
journal = "10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts",
title = "In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product",
pages = "153-152",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5993"
}

Veljković, M., Simović, M., Petrov Ivanković, A., Vukoičić, A., Banjanac, K.,& Bezbradica, D.. (2022). In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product. in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia., 152-153.
https://hdl.handle.net/21.15107/rcub_technorep_5993

Veljković M, Simović M, Petrov Ivanković A, Vukoičić A, Banjanac K, Bezbradica D. In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product. in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts. 2022;:152-153.
https://hdl.handle.net/21.15107/rcub_technorep_5993 .

Veljković, Milica, Simović, Milica, Petrov Ivanković, Anja, Vukoičić, Ana, Banjanac, Katarina, Bezbradica, Dejan, "In situ transformation of sucrose in maple syrup in order to produce fructo-oligosaccharide enriched product" in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts (2022):152-153,
https://hdl.handle.net/21.15107/rcub_technorep_5993 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Veljković, Milica
AU  - Vukoičić, Ana
AU  - Milivojević, Ana
AU  - Ćorović, Marija
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5994
AB  - According to experts, berries are a major source of phytochemicals, disease-fighting
components, which can help to boost the immune system and lower the risk of many agingrelated conditions. The simplest way to isolate these compounds is through solvent
extraction. However, this method is not entirely successful since, in addition to free
phytochemicals, there are also significant amounts of bound components that are trapped in
the cell wall. For these reasons, the addition of enzymes such as cellulases and pectinases
provides the hydrolysis of the cell wall, facilitate the release of molecules and increase the
extraction yield of phytochemical. Therefore, in this work, enzyme-assisted extraction of
lyophilized blackcurrant (LCR), a fruit that is increasingly used for pharmaceutical and
cosmetic purposes, was examined. Extraction was performed in acetate buffer pH 4.5, at 50 ̊
C using four enzymes: Pectinex® Ultra SP-L, Viscozyme® L, Cellic® CTec3, Rohapect® MC. In
the first step of the experiment, the extraction was optimized by different-time varying, from
10 to 120 min, and after choosing the optimal time, the influence of the solid-liquid ratio (1:4,
1:10, 1:40) on the extraction efficiency was examined. The progress of extraction was
monitored spectrophotometrically, through antioxidant properties, the total content of
polyphenols, flavonoids, and phenolic acids from all blackcurrant extracts and compared to
the control (extract without enzyme). Regarding the total content of polyphenols, it was
revealed that 60 min was the optimal extraction time. On the other hand, it was found that for a solid-liquid ratio of 1:4, all results were about 1.5 times higher than the control values.
In terms of enzymes, Viscozyme® and Pectinex® Ultra SP-L enabled the recovery of the highest
total phenolic content (~28 mg GAE/g LCR) and phenolic acids (~3 mg CA/g LCR), while, on the
other hand, Rohapect® MC enhanced the extraction of antioxidants (FRAP 380 µmol Fe2+/g
LCR), as well as total flavonoids (2.85 mg QE/g LCR). Obtained results open new possibilities
for blackcurrant extracts treated with different enzymes as value-added ingredients for the
cosmetic and food industry
PB  - Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia
C3  - 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
T1  - Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)
EP  - 146
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5994
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Veljković, Milica and Vukoičić, Ana and Milivojević, Ana and Ćorović, Marija and Pjanović, Rada and Bezbradica, Dejan",
year = "2022",
abstract = "According to experts, berries are a major source of phytochemicals, disease-fighting
components, which can help to boost the immune system and lower the risk of many agingrelated conditions. The simplest way to isolate these compounds is through solvent
extraction. However, this method is not entirely successful since, in addition to free
phytochemicals, there are also significant amounts of bound components that are trapped in
the cell wall. For these reasons, the addition of enzymes such as cellulases and pectinases
provides the hydrolysis of the cell wall, facilitate the release of molecules and increase the
extraction yield of phytochemical. Therefore, in this work, enzyme-assisted extraction of
lyophilized blackcurrant (LCR), a fruit that is increasingly used for pharmaceutical and
cosmetic purposes, was examined. Extraction was performed in acetate buffer pH 4.5, at 50 ̊
C using four enzymes: Pectinex® Ultra SP-L, Viscozyme® L, Cellic® CTec3, Rohapect® MC. In
the first step of the experiment, the extraction was optimized by different-time varying, from
10 to 120 min, and after choosing the optimal time, the influence of the solid-liquid ratio (1:4,
1:10, 1:40) on the extraction efficiency was examined. The progress of extraction was
monitored spectrophotometrically, through antioxidant properties, the total content of
polyphenols, flavonoids, and phenolic acids from all blackcurrant extracts and compared to
the control (extract without enzyme). Regarding the total content of polyphenols, it was
revealed that 60 min was the optimal extraction time. On the other hand, it was found that for a solid-liquid ratio of 1:4, all results were about 1.5 times higher than the control values.
In terms of enzymes, Viscozyme® and Pectinex® Ultra SP-L enabled the recovery of the highest
total phenolic content (~28 mg GAE/g LCR) and phenolic acids (~3 mg CA/g LCR), while, on the
other hand, Rohapect® MC enhanced the extraction of antioxidants (FRAP 380 µmol Fe2+/g
LCR), as well as total flavonoids (2.85 mg QE/g LCR). Obtained results open new possibilities
for blackcurrant extracts treated with different enzymes as value-added ingredients for the
cosmetic and food industry",
publisher = "Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia",
journal = "10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts",
title = "Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)",
pages = "146-145",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5994"
}

Petrov Ivanković, A., Veljković, M., Vukoičić, A., Milivojević, A., Ćorović, M., Pjanović, R.,& Bezbradica, D.. (2022). Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum). in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts
Croatian Society of Food Technologists, Biotechnologists and Nutritionists, Zagreb, Croatia., 145-146.
https://hdl.handle.net/21.15107/rcub_technorep_5994

Petrov Ivanković A, Veljković M, Vukoičić A, Milivojević A, Ćorović M, Pjanović R, Bezbradica D. Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum). in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts. 2022;:145-146.
https://hdl.handle.net/21.15107/rcub_technorep_5994 .

Petrov Ivanković, Anja, Veljković, Milica, Vukoičić, Ana, Milivojević, Ana, Ćorović, Marija, Pjanović, Rada, Bezbradica, Dejan, "Enzyme-assisted extraction of various bioactive components from blackcurrant (Ribes nigrum)" in 10th International Congress of Food Technologists, Biotechnologists and Nutritionists, Book of Abstracts (2022):145-146,
https://hdl.handle.net/21.15107/rcub_technorep_5994 .

TY  - CONF
AU  - Petrov Ivanković, Anja
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Skenderija, Jovana
AU  - Vukoičić, Ana
AU  - Pjanović, Rada
AU  - Bezbradica, Dejan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6049
AB  - According to the current definition, a prebiotic is described as “a substrate that is selectively utilized by host microorganisms conferring a health benefit”. Originally, the concept of prebiotics was defined for the gut microbiota. However, it can be also applied to modulate the composition of other microbiological populations, for example, skin microbiota. In recent years, scientific interest in the composition and function of the dermal microbiota has been growing, as it has been established that certain skin diseases are associated with dysbiosis of the skin microbiota. Consequently, for the cosmetics industry, it is of utmost importance to develop products that have a prebiotic effect and thus can provide a balanced composition of the skin microbiota. In this paper, an assessment of the galacto-oligosaccharides (GOS) prebiotic activity was performed by following the effect of the addition of these oligosaccharides on the growth of Staphylococcus epidermidis, a member of the permanent skin microbiota. Simultaneously, changes in nutrient medium composition during the fermentation were monitored using high-performance liquid chromatography. In the end, bearing in mind the future implementation of GOS in cosmetic formulations, the possibility of controlled release was determined using the Franz cell diffusion study. The obtained results showed that GOS has a stimulating effect on the growth of S. epidermidis since it has been noticed that the number of vegetative cells increases with increasing concentration of GOS up to the concentration of 5 % (w/v). Based on the change in the composition of soluble sugars in nutrient media enriched with oligosaccharides, it was confirmed that the bacteria S. epidermidis can utilize GOS. Finally, the effective diffusion coefficients of GOS, that have been incorporated into two different cosmetic formulations for previously defined optimal concentration (5 % w/v), were determined. Namely, the best results were obtained for formulation with Heliogel as a gelling agent - the effective diffusion coefficients of GOS trisaccharides (GOS3) and GOS tetrasaccharides (GOS4) were 1.904·10-6 cm2/s and 4.696·10-9 cm2/s, respectively. On the other hand, the effective diffusion coefficient of GOS3 in hydrogel Aristoflex AVC was 5.148·10-8 cm2/s, while the diffusion coefficient of GOS4 could not be determined, due to the low diffused concentrations. This indicates that GOS3 diffuse significantly faster than GOS4 and the rate and mechanism of transport of these molecules are highly dependent upon the type and characteristics of the formulation.
PB  - Faculty of Technology, University of East Sarajevo
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts
T1  - EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS
IS  - ENG-97
SP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6049
ER  - 

@conference{
author = "Petrov Ivanković, Anja and Ćorović, Marija and Milivojević, Ana and Skenderija, Jovana and Vukoičić, Ana and Pjanović, Rada and Bezbradica, Dejan",
year = "2021",
abstract = "According to the current definition, a prebiotic is described as “a substrate that is selectively utilized by host microorganisms conferring a health benefit”. Originally, the concept of prebiotics was defined for the gut microbiota. However, it can be also applied to modulate the composition of other microbiological populations, for example, skin microbiota. In recent years, scientific interest in the composition and function of the dermal microbiota has been growing, as it has been established that certain skin diseases are associated with dysbiosis of the skin microbiota. Consequently, for the cosmetics industry, it is of utmost importance to develop products that have a prebiotic effect and thus can provide a balanced composition of the skin microbiota. In this paper, an assessment of the galacto-oligosaccharides (GOS) prebiotic activity was performed by following the effect of the addition of these oligosaccharides on the growth of Staphylococcus epidermidis, a member of the permanent skin microbiota. Simultaneously, changes in nutrient medium composition during the fermentation were monitored using high-performance liquid chromatography. In the end, bearing in mind the future implementation of GOS in cosmetic formulations, the possibility of controlled release was determined using the Franz cell diffusion study. The obtained results showed that GOS has a stimulating effect on the growth of S. epidermidis since it has been noticed that the number of vegetative cells increases with increasing concentration of GOS up to the concentration of 5 % (w/v). Based on the change in the composition of soluble sugars in nutrient media enriched with oligosaccharides, it was confirmed that the bacteria S. epidermidis can utilize GOS. Finally, the effective diffusion coefficients of GOS, that have been incorporated into two different cosmetic formulations for previously defined optimal concentration (5 % w/v), were determined. Namely, the best results were obtained for formulation with Heliogel as a gelling agent - the effective diffusion coefficients of GOS trisaccharides (GOS3) and GOS tetrasaccharides (GOS4) were 1.904·10-6 cm2/s and 4.696·10-9 cm2/s, respectively. On the other hand, the effective diffusion coefficient of GOS3 in hydrogel Aristoflex AVC was 5.148·10-8 cm2/s, while the diffusion coefficient of GOS4 could not be determined, due to the low diffused concentrations. This indicates that GOS3 diffuse significantly faster than GOS4 and the rate and mechanism of transport of these molecules are highly dependent upon the type and characteristics of the formulation.",
publisher = "Faculty of Technology, University of East Sarajevo",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts",
title = "EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS",
number = "ENG-97",
pages = "140",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6049"
}

Petrov Ivanković, A., Ćorović, M., Milivojević, A., Skenderija, J., Vukoičić, A., Pjanović, R.,& Bezbradica, D.. (2021). EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS. in VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts
Faculty of Technology, University of East Sarajevo.(ENG-97), 140.
https://hdl.handle.net/21.15107/rcub_technorep_6049

Petrov Ivanković A, Ćorović M, Milivojević A, Skenderija J, Vukoičić A, Pjanović R, Bezbradica D. EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS. in VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts. 2021;(ENG-97):140.
https://hdl.handle.net/21.15107/rcub_technorep_6049 .

Petrov Ivanković, Anja, Ćorović, Marija, Milivojević, Ana, Skenderija, Jovana, Vukoičić, Ana, Pjanović, Rada, Bezbradica, Dejan, "EFFECT OF GALACTO-OLIGOSACCHARIDES ON STAPHYLOCOCCUS EPIDERMIDIS GROWTH AND EXAMINATION OF THEIR DIFFUSION FROM COSMETIC FORMULATIONS" in VII International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, Republic of Srpska Bosnia and Hercegovina, Book of abstracts, no. ENG-97 (2021):140,
https://hdl.handle.net/21.15107/rcub_technorep_6049 .

TY  - JOUR
AU  - Bebić, Jelena M.
AU  - Banjanac, Katarina M.
AU  - Ćorović, Marija M.
AU  - Milivojević, Ana D.
AU  - Simović, Milica B.
AU  - Vukoičić, Ana Ž.
AU  - Mitrović, Danica D.
AU  - Bezbradica, Dejan I.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4374
AB  - In this study, immobilization of laccase from Trametes versicolor on eight Lifetech (TM) supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech (TM) ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech (TM) ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset (R) Violet B, Lanaset (R) Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems.
AB  - U ovom radu je ispitivana i optimizovana imobilizacija lakaze producenta Trametes
versicolor na osam Lifetech™nosača, različitih karakteristika (kao što su veličina pora,
dužina “nožice” i funkcionalne grupe), za predviđenu namenu u bioremedijaciji radi
obezbojavanja industrijskih otpadnih voda. Od šest ispitanih amino-funkcionalizovanih nosača, kao najadekvatniji se pokazao porozni Lifetech™ ECR8309F nosač sa
primarnim amino grupama i C2 “nožicom”. Na ovaj nosač lakaza se vezuje formiranjem
elektrostatičkih interakcija i dobijeni preparat je imao aktivnost od 66876 U/g nosača.
Nasuprot tome, prilikom imobilizacije lakaze na epoksi/butil metakrilatni nosač
Lifetech™ ECR8285F, hidrofobnim interakcijama i kovalentnim vezama, što je
potvrđeno tretmanom imobilisanog preparata desorpcionim sredstvima, postignut je
prinos imobilizacije proteina od 60 %, a dobijena je aktivnost od 118929 U/g. Pored
toga, preparat ECR8285F-lakaza pokazao je veliku aktivnost u obezbojavanju boja
(Lanaset® Violet B, Lanaset® Blue 2R, bromtimol plavo i bromkrezol zeleno)
kombinacijom adsorpcije i enzimske razgradnje, u opsegu obezbojavanja od 88 % do
96 % nakon 4 h, sa više od 80 % obezbojavanja postignutog nakon samo 45 min.
Takođe, ovaj preparat je pokazao veliku operativnu stabilnost tokom 7 uzastopnih
ponovnih upotreba u svim ispitanim reakcionim sistemima.
PB  - Savez hemijskih inženjera
T2  - Hemijska industrija
T1  - Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes
T1  - Imobilizacija lakaze producenta Trametes versicolor na Lifetech (TM) nosače u cilju primene u razgradnji industrijskih boja
EP  - 209
IS  - 3
SP  - 197
VL  - 74
DO  - 10.2298/HEMIND200320016B
ER  - 

@article{
author = "Bebić, Jelena M. and Banjanac, Katarina M. and Ćorović, Marija M. and Milivojević, Ana D. and Simović, Milica B. and Vukoičić, Ana Ž. and Mitrović, Danica D. and Bezbradica, Dejan I.",
year = "2020",
abstract = "In this study, immobilization of laccase from Trametes versicolor on eight Lifetech (TM) supports, with different characteristics (pore size, length of the spacer arm and functional groups), was studied and optimized for intended use in bioremediation for decolorization of industrial wastewaters. Out of six tested amino-functionalized supports, the most promising carrier was proved to be porous Lifetech (TM) ECR8309F with primary amino groups and a C2 spacer arm. Onto this support, laccase is attached by forming electrostatic interactions so that the most active preparation has shown the activity of 66876 U/g support. On the other hand, during immobilization of laccase on epoxy-functionalized Lifetech (TM) ECR8285F, via hydrophobic interactions and covalent bonding confirmed by a desorption assay, immobilization yield of 60 % and the activity of 118929 U/g were accomplished. Furthermore, immobilized enzyme on this support showed high capacity for decolorization of dyes (Lanaset (R) Violet B, Lanaset (R) Blue 2R, bromothymol blue and bromocresol green), by combination of both adsorption and enzyme degradation. Decolorization was in the range of 88 to 96 % after 4 h, with more than 80 % achieved after only 45 min. Also, this preparation demonstrated high operational stability during seven consecutive reuses in all examined dye reaction systems., U ovom radu je ispitivana i optimizovana imobilizacija lakaze producenta Trametes
versicolor na osam Lifetech™nosača, različitih karakteristika (kao što su veličina pora,
dužina “nožice” i funkcionalne grupe), za predviđenu namenu u bioremedijaciji radi
obezbojavanja industrijskih otpadnih voda. Od šest ispitanih amino-funkcionalizovanih nosača, kao najadekvatniji se pokazao porozni Lifetech™ ECR8309F nosač sa
primarnim amino grupama i C2 “nožicom”. Na ovaj nosač lakaza se vezuje formiranjem
elektrostatičkih interakcija i dobijeni preparat je imao aktivnost od 66876 U/g nosača.
Nasuprot tome, prilikom imobilizacije lakaze na epoksi/butil metakrilatni nosač
Lifetech™ ECR8285F, hidrofobnim interakcijama i kovalentnim vezama, što je
potvrđeno tretmanom imobilisanog preparata desorpcionim sredstvima, postignut je
prinos imobilizacije proteina od 60 %, a dobijena je aktivnost od 118929 U/g. Pored
toga, preparat ECR8285F-lakaza pokazao je veliku aktivnost u obezbojavanju boja
(Lanaset® Violet B, Lanaset® Blue 2R, bromtimol plavo i bromkrezol zeleno)
kombinacijom adsorpcije i enzimske razgradnje, u opsegu obezbojavanja od 88 % do
96 % nakon 4 h, sa više od 80 % obezbojavanja postignutog nakon samo 45 min.
Takođe, ovaj preparat je pokazao veliku operativnu stabilnost tokom 7 uzastopnih
ponovnih upotreba u svim ispitanim reakcionim sistemima.",
publisher = "Savez hemijskih inženjera",
journal = "Hemijska industrija",
title = "Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes, Imobilizacija lakaze producenta Trametes versicolor na Lifetech (TM) nosače u cilju primene u razgradnji industrijskih boja",
pages = "209-197",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200320016B"
}

Bebić, J. M., Banjanac, K. M., Ćorović, M. M., Milivojević, A. D., Simović, M. B., Vukoičić, A. Ž., Mitrović, D. D.,& Bezbradica, D. I.. (2020). Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes. in Hemijska industrija
Savez hemijskih inženjera., 74(3), 197-209.
https://doi.org/10.2298/HEMIND200320016B

Bebić JM, Banjanac KM, Ćorović MM, Milivojević AD, Simović MB, Vukoičić AŽ, Mitrović DD, Bezbradica DI. Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes. in Hemijska industrija. 2020;74(3):197-209.
doi:10.2298/HEMIND200320016B .

Bebić, Jelena M., Banjanac, Katarina M., Ćorović, Marija M., Milivojević, Ana D., Simović, Milica B., Vukoičić, Ana Ž., Mitrović, Danica D., Bezbradica, Dejan I., "Immobilization of laccase from Trametes versicolor on Lifetech (TM) supports for applications in degradation of industrial dyes" in Hemijska industrija, 74, no. 3 (2020):197-209,
https://doi.org/10.2298/HEMIND200320016B . .