@conference{
author = "Nešović, Katarina and Janković, Ana and Surudžić, Rade and Vukašinović-Sekulić, Maja and Kojić, Vesna and Radetić, Tamara and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Recent advances in biomaterials science are increasingly focused
on (re)search for next-generation soft tissue regeneration solutions.
Hydrogel-based materials are becoming a focal point to address
many tissue engineering requirements, possessing exceptional biocompatibility,
with tailorable shape, structure and mechanical
properties, as well as infinite possibilities drug delivery. The widespread
antibiotics use has led to aggravated resistance issues in many
bacterial strains, so the focus is increasingly shifted towards alternative
antibacterial solutions such as silver nanoparticles (AgNPs).
In this work, we have developed novel composite materials based on
poly(vinyl alcohol)/chitosan (PVA/CHI) and poly(vinyl alcohol)/
chitosan/graphene (PVA/CHI/Gr) hydrogels with silver nanoparticles
obtained through green in situ constant-voltage electrochemical
synthesis. The obtained AgNPs’ properties, sizes and size
distributions were examined using UV-visible spectroscopy, dynamic
light scattering and transmission electron microscopy. In vitro
swelling and silver release kinetics were monitored in the simulated
physiological conditions (pH7.4, 37 C). The obtained release and
swelling isotherms were fitted with several theoretical models that
helped discern the mechanisms of these processes. Tensile testing
was carried out to examine the composites’ mechanical behavior and
elasticity – important properties for soft tissue engineering materials.
Finally, the excellent antibacterial activity of the obtained silver/
poly(vinyl alcohol)/chitosan (Ag/PVA/CHI) and silver/poly(vinyl
alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) hydrogels against
Staphylococcus aureus and Escherichia coli was confirmed quantitatively
by colony counting method, and their non-toxicity was
verified towards two model fibroblast cell lines (MRC-5 and L929)
utilizing the MTT assay. Through thorough characterization, the
obtained nanocomposite hydrogel materials were identified as strong
candidates for active antibacterial soft tissue engineering materials.",
publisher = "Mary Ann Liebert, Inc.",
journal = "Tissue Engineering Part A",
title = "Silver Nanoparticles-Loaded Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Obtained by Electrochemical Synthesis",
number = "S1",
pages = "S-132",
volume = "28",
doi = "10.1089/ten.tea.2022.29025.abstracts"
}