TY  - JOUR
AU  - Misan, Aleksandra
AU  - Sarić, Bojana
AU  - Milovanović, Ivan
AU  - Jovanov, Pavle
AU  - Sedej, Ivana
AU  - Tadić, Vanja
AU  - Mandić, Anamarija
AU  - Sakac, Marijana
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3712
AB  - Due to a high content of rutin (2-10%), dried buckwheat leaf and flower (DBLF) formulations were shown to be efficient in the treatment of vascular diseases. In order to find a cost effective way for the extraction of antioxidants, the effects of ethanol/water ratio and temperature on the extraction efficiency of phenolic compounds and the mechanisms of antioxidant action of the extracts were tested. Extraction with ethanol/water mixture (80:20, v/v) for 24 h at room temperature, after the mixture was just brought to boil was demonstrated to be an efficient and cheap way for obtaining a high yield of rutin (49.94 +/- 0.623 mg/g DBLF). The most abundant phenolic compounds in DBLF extracts were rutin and chlorogenic acid. Flavonoids, especially rutin, were shown to be the most responsible for the antioxidant activity in all investigated lipid model systems, acting as free radical scavengers, electron-donating substances and chelators of iron ions. In beta-carotene bleaching tests, the extracts with the highest activity were as efficient as BHT (butylated hydroxytoluole). Regarding the results of antihemolytic and Schaal oven tests, the extracts demonstrated remarkable ability to inhibit the oxidative destruction of erythrocytes and to prolong the beginning of the oxidation process in sunflower oil.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Phenolic profile and antioxidant properties of dried buckwheat leaf and flower extracts
EP  - 47
IS  - 1
SP  - 39
VL  - 23
DO  - 10.2298/CICEQ150704004M
ER  - 

@article{
author = "Misan, Aleksandra and Sarić, Bojana and Milovanović, Ivan and Jovanov, Pavle and Sedej, Ivana and Tadić, Vanja and Mandić, Anamarija and Sakac, Marijana",
year = "2017",
abstract = "Due to a high content of rutin (2-10%), dried buckwheat leaf and flower (DBLF) formulations were shown to be efficient in the treatment of vascular diseases. In order to find a cost effective way for the extraction of antioxidants, the effects of ethanol/water ratio and temperature on the extraction efficiency of phenolic compounds and the mechanisms of antioxidant action of the extracts were tested. Extraction with ethanol/water mixture (80:20, v/v) for 24 h at room temperature, after the mixture was just brought to boil was demonstrated to be an efficient and cheap way for obtaining a high yield of rutin (49.94 +/- 0.623 mg/g DBLF). The most abundant phenolic compounds in DBLF extracts were rutin and chlorogenic acid. Flavonoids, especially rutin, were shown to be the most responsible for the antioxidant activity in all investigated lipid model systems, acting as free radical scavengers, electron-donating substances and chelators of iron ions. In beta-carotene bleaching tests, the extracts with the highest activity were as efficient as BHT (butylated hydroxytoluole). Regarding the results of antihemolytic and Schaal oven tests, the extracts demonstrated remarkable ability to inhibit the oxidative destruction of erythrocytes and to prolong the beginning of the oxidation process in sunflower oil.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Phenolic profile and antioxidant properties of dried buckwheat leaf and flower extracts",
pages = "47-39",
number = "1",
volume = "23",
doi = "10.2298/CICEQ150704004M"
}

Misan, A., Sarić, B., Milovanović, I., Jovanov, P., Sedej, I., Tadić, V., Mandić, A.,& Sakac, M.. (2017). Phenolic profile and antioxidant properties of dried buckwheat leaf and flower extracts. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 23(1), 39-47.
https://doi.org/10.2298/CICEQ150704004M

Misan A, Sarić B, Milovanović I, Jovanov P, Sedej I, Tadić V, Mandić A, Sakac M. Phenolic profile and antioxidant properties of dried buckwheat leaf and flower extracts. in Chemical Industry & Chemical Engineering Quarterly. 2017;23(1):39-47.
doi:10.2298/CICEQ150704004M .

Misan, Aleksandra, Sarić, Bojana, Milovanović, Ivan, Jovanov, Pavle, Sedej, Ivana, Tadić, Vanja, Mandić, Anamarija, Sakac, Marijana, "Phenolic profile and antioxidant properties of dried buckwheat leaf and flower extracts" in Chemical Industry & Chemical Engineering Quarterly, 23, no. 1 (2017):39-47,
https://doi.org/10.2298/CICEQ150704004M . .

TY  - JOUR
AU  - Vajdle, Olga
AU  - Guzsvány, Valéria
AU  - Skorić, Dušan
AU  - Anojčić, Jasmina
AU  - Jovanov, Pavle
AU  - Avramov-Ivić, Milka
AU  - Csanadi, Janos
AU  - Kónya, Zoltán
AU  - Petrović, Slobodan
AU  - Bobrowski, Andrzej
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3465
AB  - Erythromycin, a macrolide antibiotic, has similar antimicrobial spectrum to penicillin and it is widely used, especially in the treatment of patients who are allergic to penicillin. In this work, the application of a renewable silver-amalgam film electrode (Hg(Ag)FE) for the characterization and determination of erythromycin ethylsuccinate (EES), a widely used esterified form of this antibiotic, by means of cyclic voltammetry (CV) and square wave voltammetry (SWV) is presented. In the aqueous Britton-Robinson buffer (pH 5.0-9.0) that served as the supporting electrolyte, one reduction peak of EES was observed in the investigated potential range between -0.75 V and -1.80 V vs SCE, with peak potential maxima ranging from -1.59 V to -1.70 V, which strongly depended on the applied pH, as did the peak shape. For the analytical purposes the pH of 7.0 was selected, since in this electrolyte the EES peak was well-shaped and separated from the background current of the supporting electrolyte in both cases; in the direct cathodic SWV and in the case of square wave adsorptive stripping voltammetry (SW-AdSV). It was established, by the E-p-pH correlation, that protons strongly influenced the electrochemical reduction of EES. The CVs recorded between 0.025-0.50 V s (1) at pH 7.0 confirmed that the electrode reaction is adsorption-controlled. Based on the series of H-1 NMR measurements it is proved that the tertiary amino group of EES is mainly in its protonated form at pH 7.0 which may lead, at appropriate accumulation potential and time, to the favored adsorption of the target ionic form of the analyte improving on such way the sensitivity of the SW-AdSV method. The optimized procedures resulted in stable SWV responses with good linear correlation in the EES concentration range from 4.53 to 29.8 mu g mL (1) (LOD = 1.36 mu g mL (1)), and from 0.69 mu g mL (1) to 2.44 mu g mL (1) (LOD 0.21 mu g mL (1)) in the case of optimized SW-AdSV. The relative standard deviation is below 1.5%. The reliability of the elaborated procedures and thus the accuracy of the obtained results were validated by comparing them with those obtained by means of HPLC-DAD measurements. The direct cathodic SWV method was successfully applied for the determination of EES in the pharmaceutical preparation Eritromicin (R), while SW-AdSV was applied in the case of the spiked urine sample. In both cases, the standard addition method was used.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Electrochimica Acta
T1  - Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation
EP  - 54
SP  - 44
VL  - 191
DO  - 10.1016/j.electacta.2015.12.207
ER  - 

@article{
author = "Vajdle, Olga and Guzsvány, Valéria and Skorić, Dušan and Anojčić, Jasmina and Jovanov, Pavle and Avramov-Ivić, Milka and Csanadi, Janos and Kónya, Zoltán and Petrović, Slobodan and Bobrowski, Andrzej",
year = "2016",
abstract = "Erythromycin, a macrolide antibiotic, has similar antimicrobial spectrum to penicillin and it is widely used, especially in the treatment of patients who are allergic to penicillin. In this work, the application of a renewable silver-amalgam film electrode (Hg(Ag)FE) for the characterization and determination of erythromycin ethylsuccinate (EES), a widely used esterified form of this antibiotic, by means of cyclic voltammetry (CV) and square wave voltammetry (SWV) is presented. In the aqueous Britton-Robinson buffer (pH 5.0-9.0) that served as the supporting electrolyte, one reduction peak of EES was observed in the investigated potential range between -0.75 V and -1.80 V vs SCE, with peak potential maxima ranging from -1.59 V to -1.70 V, which strongly depended on the applied pH, as did the peak shape. For the analytical purposes the pH of 7.0 was selected, since in this electrolyte the EES peak was well-shaped and separated from the background current of the supporting electrolyte in both cases; in the direct cathodic SWV and in the case of square wave adsorptive stripping voltammetry (SW-AdSV). It was established, by the E-p-pH correlation, that protons strongly influenced the electrochemical reduction of EES. The CVs recorded between 0.025-0.50 V s (1) at pH 7.0 confirmed that the electrode reaction is adsorption-controlled. Based on the series of H-1 NMR measurements it is proved that the tertiary amino group of EES is mainly in its protonated form at pH 7.0 which may lead, at appropriate accumulation potential and time, to the favored adsorption of the target ionic form of the analyte improving on such way the sensitivity of the SW-AdSV method. The optimized procedures resulted in stable SWV responses with good linear correlation in the EES concentration range from 4.53 to 29.8 mu g mL (1) (LOD = 1.36 mu g mL (1)), and from 0.69 mu g mL (1) to 2.44 mu g mL (1) (LOD 0.21 mu g mL (1)) in the case of optimized SW-AdSV. The relative standard deviation is below 1.5%. The reliability of the elaborated procedures and thus the accuracy of the obtained results were validated by comparing them with those obtained by means of HPLC-DAD measurements. The direct cathodic SWV method was successfully applied for the determination of EES in the pharmaceutical preparation Eritromicin (R), while SW-AdSV was applied in the case of the spiked urine sample. In both cases, the standard addition method was used.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Electrochimica Acta",
title = "Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation",
pages = "54-44",
volume = "191",
doi = "10.1016/j.electacta.2015.12.207"
}

Vajdle, O., Guzsvány, V., Skorić, D., Anojčić, J., Jovanov, P., Avramov-Ivić, M., Csanadi, J., Kónya, Z., Petrović, S.,& Bobrowski, A.. (2016). Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation. in Electrochimica Acta
Pergamon-Elsevier Science Ltd, Oxford., 191, 44-54.
https://doi.org/10.1016/j.electacta.2015.12.207

Vajdle O, Guzsvány V, Skorić D, Anojčić J, Jovanov P, Avramov-Ivić M, Csanadi J, Kónya Z, Petrović S, Bobrowski A. Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation. in Electrochimica Acta. 2016;191:44-54.
doi:10.1016/j.electacta.2015.12.207 .

Vajdle, Olga, Guzsvány, Valéria, Skorić, Dušan, Anojčić, Jasmina, Jovanov, Pavle, Avramov-Ivić, Milka, Csanadi, Janos, Kónya, Zoltán, Petrović, Slobodan, Bobrowski, Andrzej, "Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation" in Electrochimica Acta, 191 (2016):44-54,
https://doi.org/10.1016/j.electacta.2015.12.207 . .