TY  - JOUR
AU  - Stanišić, Marija D.
AU  - Popović Kokar, Nikolina
AU  - Ristić, Predrag
AU  - Balaž, Ana Marija
AU  - Ognjanović, Miloš
AU  - Đokić, Veljko R.
AU  - Prodanović, Radivoje
AU  - Todorović, Tamara R.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5269
AB  - Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites.
PB  - MDPI
T2  - Polymers
T1  - The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance
IS  - 22
SP  - 4834
VL  - 14
DO  - 10.3390/polym14224834
ER  - 

@article{
author = "Stanišić, Marija D. and Popović Kokar, Nikolina and Ristić, Predrag and Balaž, Ana Marija and Ognjanović, Miloš and Đokić, Veljko R. and Prodanović, Radivoje and Todorović, Tamara R.",
year = "2022",
abstract = "Many articles in the literature deal with horseradish peroxidase (HRP) biomineralization, but none pay attention to the isoenzyme composition of commercial HRP or the influence of the carbohydrate component of the protein molecule on the biomineralization process. To study the impact of these factors, we performed periodate oxidation of commercial HRP and a purified HRP-C isoform for biomineralization within ZIF-8. With purified HRP, enzyme@ZIF-8 biocomposites with higher activity were obtained, while periodate oxidation of the carbohydrate component of both commercial HRP and purified HRP-C yields biocomposites with very high activity in acetate buffer that does not degrade the ZIF-8 structure. Using acetate instead of phosphate buffer can prevent the false high activity of HRP@ZIF-8 biocomposites caused by the degradation of ZIF-8 coating. At the same time, purification and especially oxidation of the carbohydrate component of enzymes prior to biomineralization lead to significantly improved activity of the biocomposites.",
publisher = "MDPI",
journal = "Polymers",
title = "The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance",
number = "22",
pages = "4834",
volume = "14",
doi = "10.3390/polym14224834"
}

Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in Polymers
MDPI., 14(22), 4834.
https://doi.org/10.3390/polym14224834

Stanišić MD, Popović Kokar N, Ristić P, Balaž AM, Ognjanović M, Đokić VR, Prodanović R, Todorović TR. The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in Polymers. 2022;14(22):4834.
doi:10.3390/polym14224834 .

Stanišić, Marija D., Popović Kokar, Nikolina, Ristić, Predrag, Balaž, Ana Marija, Ognjanović, Miloš, Đokić, Veljko R., Prodanović, Radivoje, Todorović, Tamara R., "The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance" in Polymers, 14, no. 22 (2022):4834,
https://doi.org/10.3390/polym14224834 . .

TY  - DATA
AU  - Stanišić, Marija D.
AU  - Popović Kokar, Nikolina
AU  - Ristić, Predrag
AU  - Balaž, Ana Marija
AU  - Ognjanović, Miloš
AU  - Đokić, Veljko R.
AU  - Prodanović, Radivoje
AU  - Todorović, Tamara R.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5951
T2  - Polymers
T1  - Primary research data for the article: Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in PolymersMDPI., 14(22), 4834.https://doi.org/10.3390/polym14224834
IS  - 22
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5740
ER  - 

@misc{
author = "Stanišić, Marija D. and Popović Kokar, Nikolina and Ristić, Predrag and Balaž, Ana Marija and Ognjanović, Miloš and Đokić, Veljko R. and Prodanović, Radivoje and Todorović, Tamara R.",
year = "2022",
journal = "Polymers",
title = "Primary research data for the article: Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in PolymersMDPI., 14(22), 4834.https://doi.org/10.3390/polym14224834",
number = "22",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5740"
}

Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). Primary research data for the article: Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in PolymersMDPI., 14(22), 4834.https://doi.org/10.3390/polym14224834. in Polymers, 14(22).
https://hdl.handle.net/21.15107/rcub_cherry_5740

Stanišić MD, Popović Kokar N, Ristić P, Balaž AM, Ognjanović M, Đokić VR, Prodanović R, Todorović TR. Primary research data for the article: Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in PolymersMDPI., 14(22), 4834.https://doi.org/10.3390/polym14224834. in Polymers. 2022;14(22).
https://hdl.handle.net/21.15107/rcub_cherry_5740 .

Stanišić, Marija D., Popović Kokar, Nikolina, Ristić, Predrag, Balaž, Ana Marija, Ognjanović, Miloš, Đokić, Veljko R., Prodanović, Radivoje, Todorović, Tamara R., "Primary research data for the article: Stanišić, M. D., Popović Kokar, N., Ristić, P., Balaž, A. M., Ognjanović, M., Đokić, V. R., Prodanović, R.,& Todorović, T. R.. (2022). The Influence of Isoenzyme Composition and Chemical Modification on Horseradish Peroxidase@ZIF-8 Biocomposite Performance. in PolymersMDPI., 14(22), 4834.https://doi.org/10.3390/polym14224834" in Polymers, 14, no. 22 (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5740 .

TY  - CONF
AU  - Ristić, Predrag
AU  - Pavlović, Pavle
AU  - Stanišić, Marija
AU  - Prodanović, Radivoje
AU  - Ognjanović, Miloš
AU  - Đokić, Veljko
AU  - Todorović, Tamara
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5954
AB  - Metal-organic frameworks (MOFs) are a class of inorganic materials with potential application in gas adsorption, biosensitization, biocatalysis, and drug delivery [1]. Zeolitic imidazolate frame-works (ZIFs) are a subclass of MOFs, particularly suitable for enzyme immobilization by biomi-metic mineralization [1]. ZIF-8 consisting of tetrahedral Zn(II) ions bridged via 2-methylimidazole ligands (mIM) is most commonly used for this purpose. However, the topology and morphology of ZIF-8 crystals vary depending on the experimental synthesis conditions. In addition, during the process of biomimetic mineralization, the biocomposite composed of the enzyme immobilized in ZIF-8 is washed with buffers and detergents to remove excess of an adsorbed enzyme, which can lead to the chemical transformation of the surface and undesirable release of the enzyme. There-fore, the influence of anion nature on the topology and morphology of ZIF-8 was investigated in this work, and the stability of ZIF-8 crystallites was tested in acetate buffer (0.1 M; pH = 5.5) and sodium dodecyl sulfate solution (ω = 10%). Crystal morphology was monitored by scanning elec-tron microscopy, while topology was determined using powder X-ray diffraction.
PB  - Beograd : Srpsko kristalografsko društvo
C3  - XXVII Konferencija Srpskog kristalografskog društva, 16-17. Septembar 2021, Kragujevac
T1  - Uticaj anjona, pufera i detergenata na topologiju i morfologiju ZIF-8 kristalita
EP  - 65
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5750
ER  - 

@conference{
author = "Ristić, Predrag and Pavlović, Pavle and Stanišić, Marija and Prodanović, Radivoje and Ognjanović, Miloš and Đokić, Veljko and Todorović, Tamara",
year = "2021",
abstract = "Metal-organic frameworks (MOFs) are a class of inorganic materials with potential application in gas adsorption, biosensitization, biocatalysis, and drug delivery [1]. Zeolitic imidazolate frame-works (ZIFs) are a subclass of MOFs, particularly suitable for enzyme immobilization by biomi-metic mineralization [1]. ZIF-8 consisting of tetrahedral Zn(II) ions bridged via 2-methylimidazole ligands (mIM) is most commonly used for this purpose. However, the topology and morphology of ZIF-8 crystals vary depending on the experimental synthesis conditions. In addition, during the process of biomimetic mineralization, the biocomposite composed of the enzyme immobilized in ZIF-8 is washed with buffers and detergents to remove excess of an adsorbed enzyme, which can lead to the chemical transformation of the surface and undesirable release of the enzyme. There-fore, the influence of anion nature on the topology and morphology of ZIF-8 was investigated in this work, and the stability of ZIF-8 crystallites was tested in acetate buffer (0.1 M; pH = 5.5) and sodium dodecyl sulfate solution (ω = 10%). Crystal morphology was monitored by scanning elec-tron microscopy, while topology was determined using powder X-ray diffraction.",
publisher = "Beograd : Srpsko kristalografsko društvo",
journal = "XXVII Konferencija Srpskog kristalografskog društva, 16-17. Septembar 2021, Kragujevac",
title = "Uticaj anjona, pufera i detergenata na topologiju i morfologiju ZIF-8 kristalita",
pages = "65-64",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5750"
}

Ristić, P., Pavlović, P., Stanišić, M., Prodanović, R., Ognjanović, M., Đokić, V.,& Todorović, T.. (2021). Uticaj anjona, pufera i detergenata na topologiju i morfologiju ZIF-8 kristalita. in XXVII Konferencija Srpskog kristalografskog društva, 16-17. Septembar 2021, Kragujevac
Beograd : Srpsko kristalografsko društvo., 64-65.
https://hdl.handle.net/21.15107/rcub_cherry_5750

Ristić P, Pavlović P, Stanišić M, Prodanović R, Ognjanović M, Đokić V, Todorović T. Uticaj anjona, pufera i detergenata na topologiju i morfologiju ZIF-8 kristalita. in XXVII Konferencija Srpskog kristalografskog društva, 16-17. Septembar 2021, Kragujevac. 2021;:64-65.
https://hdl.handle.net/21.15107/rcub_cherry_5750 .

Ristić, Predrag, Pavlović, Pavle, Stanišić, Marija, Prodanović, Radivoje, Ognjanović, Miloš, Đokić, Veljko, Todorović, Tamara, "Uticaj anjona, pufera i detergenata na topologiju i morfologiju ZIF-8 kristalita" in XXVII Konferencija Srpskog kristalografskog društva, 16-17. Septembar 2021, Kragujevac (2021):64-65,
https://hdl.handle.net/21.15107/rcub_cherry_5750 .