TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Plavšić, Milenko
AU  - Uskoković, Dragan
PY  - 2001
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5350
AB  - Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C=O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found. Copyright © 2001 Elsevier Science Ltd.

Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C double bond O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found.
PB  - Elsevier Science Ltd
T2  - Biomaterials
T1  - A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy
EP  - 575
IS  - 6
SP  - 571
VL  - 22
DO  - 10.1016/S0142-9612(00)00215-5
ER  - 

@article{
author = "Ignjatović, Nenad and Savić, Vojin and Najman, Stevo and Plavšić, Milenko and Uskoković, Dragan",
year = "2001",
abstract = "Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C=O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found. Copyright © 2001 Elsevier Science Ltd.

Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C double bond O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found.",
publisher = "Elsevier Science Ltd",
journal = "Biomaterials",
title = "A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy",
pages = "575-571",
number = "6",
volume = "22",
doi = "10.1016/S0142-9612(00)00215-5"
}

Ignjatović, N., Savić, V., Najman, S., Plavšić, M.,& Uskoković, D.. (2001). A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy. in Biomaterials
Elsevier Science Ltd., 22(6), 571-575.
https://doi.org/10.1016/S0142-9612(00)00215-5

Ignjatović N, Savić V, Najman S, Plavšić M, Uskoković D. A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy. in Biomaterials. 2001;22(6):571-575.
doi:10.1016/S0142-9612(00)00215-5 .

Ignjatović, Nenad, Savić, Vojin, Najman, Stevo, Plavšić, Milenko, Uskoković, Dragan, "A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy" in Biomaterials, 22, no. 6 (2001):571-575,
https://doi.org/10.1016/S0142-9612(00)00215-5 . .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Plavšić, Milenko
AU  - Najman, Stevo
AU  - Savić, Vojin
AU  - Uskoković, Dragan
PY  - 2000
UR  - https://dais.sanu.ac.rs/123456789/3859
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5313
AB  - Hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial with PLLA of 50,000 and 430,000 g/mole molecular weight was studied in vivo. The biocomposite with PLLA of both molecular weights was implanted into mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After one week of testing in vivo, the implanted samples gave spectra in which absorption bands arising from new-formed functional groups of amine and peptide can be seen. Analysis of the spectra revealed faster formation of peptide compounds when the biocomposite with PLLA of lower molecular weight was used. After 3 weeks, the spectra of the biocomposite of both compositions were registered with pronounced absorption bands at about 3420 and 1650 cm-1 assigned to new-generated collagen, a component of the extracellular connective-tissue matrix. Also, in the case of the biocomposite sample with PLLA of lower molecular weight, disappearance of the previously present absorption band at about 1760 cm-1 originating from the C double bond O group of PLLA indicates complete bioresorption of the PLLA used. Analysis of the microstructures of the sample surfaces by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase in the system with PLLA of lower molecular weight and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial alone were found.
PB  - Uetikon-Zuerich : Trans Tech Publ
C3  - Trends in Advanced Materials and Processes
T1  - Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy
EP  - 150
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_dais_3859
ER  - 

@conference{
author = "Ignjatović, Nenad and Plavšić, Milenko and Najman, Stevo and Savić, Vojin and Uskoković, Dragan",
year = "2000",
abstract = "Hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial with PLLA of 50,000 and 430,000 g/mole molecular weight was studied in vivo. The biocomposite with PLLA of both molecular weights was implanted into mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After one week of testing in vivo, the implanted samples gave spectra in which absorption bands arising from new-formed functional groups of amine and peptide can be seen. Analysis of the spectra revealed faster formation of peptide compounds when the biocomposite with PLLA of lower molecular weight was used. After 3 weeks, the spectra of the biocomposite of both compositions were registered with pronounced absorption bands at about 3420 and 1650 cm-1 assigned to new-generated collagen, a component of the extracellular connective-tissue matrix. Also, in the case of the biocomposite sample with PLLA of lower molecular weight, disappearance of the previously present absorption band at about 1760 cm-1 originating from the C double bond O group of PLLA indicates complete bioresorption of the PLLA used. Analysis of the microstructures of the sample surfaces by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase in the system with PLLA of lower molecular weight and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial alone were found.",
publisher = "Uetikon-Zuerich : Trans Tech Publ",
journal = "Trends in Advanced Materials and Processes",
title = "Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy",
pages = "150-143",
url = "https://hdl.handle.net/21.15107/rcub_dais_3859"
}

Ignjatović, N., Plavšić, M., Najman, S., Savić, V.,& Uskoković, D.. (2000). Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy. in Trends in Advanced Materials and Processes
Uetikon-Zuerich : Trans Tech Publ., 143-150.
https://hdl.handle.net/21.15107/rcub_dais_3859

Ignjatović N, Plavšić M, Najman S, Savić V, Uskoković D. Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy. in Trends in Advanced Materials and Processes. 2000;:143-150.
https://hdl.handle.net/21.15107/rcub_dais_3859 .

Ignjatović, Nenad, Plavšić, Milenko, Najman, Stevo, Savić, Vojin, Uskoković, Dragan, "Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy" in Trends in Advanced Materials and Processes (2000):143-150,
https://hdl.handle.net/21.15107/rcub_dais_3859 .