TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Miljković, Miona
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Filipović, Jovanka
AU  - Tomić, Simonida
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3709
AB  - The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.
PB  - Inst Chemical Engineers, Rugby
T2  - Chemical Engineering Research & Design
T1  - Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels
EP  - 380
SP  - 368
VL  - 121
DO  - 10.1016/j.cherd.2017.03.030
ER  - 

@article{
author = "Krezović, Bojana D. and Miljković, Miona and Stojanović, Sanja T. and Najman, Stevo and Filipović, Jovanka and Tomić, Simonida",
year = "2017",
abstract = "The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Chemical Engineering Research & Design",
title = "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels",
pages = "380-368",
volume = "121",
doi = "10.1016/j.cherd.2017.03.030"
}

Krezović, B. D., Miljković, M., Stojanović, S. T., Najman, S., Filipović, J.,& Tomić, S.. (2017). Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design
Inst Chemical Engineers, Rugby., 121, 368-380.
https://doi.org/10.1016/j.cherd.2017.03.030

Krezović BD, Miljković M, Stojanović ST, Najman S, Filipović J, Tomić S. Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design. 2017;121:368-380.
doi:10.1016/j.cherd.2017.03.030 .

Krezović, Bojana D., Miljković, Miona, Stojanović, Sanja T., Najman, Stevo, Filipović, Jovanka, Tomić, Simonida, "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels" in Chemical Engineering Research & Design, 121 (2017):368-380,
https://doi.org/10.1016/j.cherd.2017.03.030 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Filipović, Jovanka
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3409
AB  - A series of dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) (P(HPA/IA)) hydrogels were synthesized and evaluated as potential highly effective antiproliferative drug delivery system. Investigated hydrophobic antiproliferative agent, Ni(II) complex with Oxaprozin, was successfully synthesized and efficiently loaded into the'"intelligent" P(HPA/IA) hydrogels. Swelling studies showed that loaded agent did not annul pH- and temperature-sensitivity of the investigated hydrogels. In vitro antiproliferative activity of investigated complex against human cervical (HeLa) and melanoma cancer (FemX) cell lines was tested. The results of in vitro release study at different pH values confirmed synthesized hydrogels loaded with investigated complex as a highly effective pH-triggered drug delivery system for the advanced anticancer therapy as well as for the targeted treatment of intestine/colon cancers.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin
EP  - 217
SP  - 214
VL  - 163
DO  - 10.1016/j.matlet.2015.10.078
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Filipović, Jovanka and Ušćumlić, Gordana and Tomić, Simonida",
year = "2016",
abstract = "A series of dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) (P(HPA/IA)) hydrogels were synthesized and evaluated as potential highly effective antiproliferative drug delivery system. Investigated hydrophobic antiproliferative agent, Ni(II) complex with Oxaprozin, was successfully synthesized and efficiently loaded into the'"intelligent" P(HPA/IA) hydrogels. Swelling studies showed that loaded agent did not annul pH- and temperature-sensitivity of the investigated hydrogels. In vitro antiproliferative activity of investigated complex against human cervical (HeLa) and melanoma cancer (FemX) cell lines was tested. The results of in vitro release study at different pH values confirmed synthesized hydrogels loaded with investigated complex as a highly effective pH-triggered drug delivery system for the advanced anticancer therapy as well as for the targeted treatment of intestine/colon cancers.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin",
pages = "217-214",
volume = "163",
doi = "10.1016/j.matlet.2015.10.078"
}

Babić, M., Božić, B., Božić, B., Filipović, J., Ušćumlić, G.,& Tomić, S.. (2016). Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin. in Materials Letters
Elsevier, Amsterdam., 163, 214-217.
https://doi.org/10.1016/j.matlet.2015.10.078

Babić M, Božić B, Božić B, Filipović J, Ušćumlić G, Tomić S. Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin. in Materials Letters. 2016;163:214-217.
doi:10.1016/j.matlet.2015.10.078 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Filipović, Jovanka, Ušćumlić, Gordana, Tomić, Simonida, "Evaluation of novel antiproliferative controlled drug delivery system based on poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels and nickel complex with Oxaprozin" in Materials Letters, 163 (2016):214-217,
https://doi.org/10.1016/j.matlet.2015.10.078 . .

TY  - CONF
AU  - Tomić, Simonida
AU  - Babić, Marija
AU  - Vuković, Jovana
AU  - Perišić, Marija D.
AU  - Filipović, Vuk
AU  - Davidović, Slađana
AU  - Filipović, Jovanka M.
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3296
AB  - In this study, superporous hydrogels were synthesized by free radical polymerization of 2-hydroxyethyl methacrylate without and in the presence of gelatin. Highly porous hydrogel structures were obtained by two different techniques: using a gas blowing agent, sodium bicarbonate, and a cryogenic treatment followed by freeze-drying. After the gel synthesis, gelatin molecules were covalently immobilised onto PHEMA via glytaraldehyde activation. All samples were characterized for morphological, mechanical, swelling and antibacterial properties. The results obtained show that samples with gelatin show better properties in comparison with PHEMA samples, which make these materials highly attractive for developing hydrogel scaffolds for tissue regeneration.
PB  - Amer Inst Physics, Melville
C3  - VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology
T1  - 2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration
SP  - 4949668
VL  - 1736
DO  - 10.1063/1.4949668
ER  - 

@conference{
author = "Tomić, Simonida and Babić, Marija and Vuković, Jovana and Perišić, Marija D. and Filipović, Vuk and Davidović, Slađana and Filipović, Jovanka M.",
year = "2016",
abstract = "In this study, superporous hydrogels were synthesized by free radical polymerization of 2-hydroxyethyl methacrylate without and in the presence of gelatin. Highly porous hydrogel structures were obtained by two different techniques: using a gas blowing agent, sodium bicarbonate, and a cryogenic treatment followed by freeze-drying. After the gel synthesis, gelatin molecules were covalently immobilised onto PHEMA via glytaraldehyde activation. All samples were characterized for morphological, mechanical, swelling and antibacterial properties. The results obtained show that samples with gelatin show better properties in comparison with PHEMA samples, which make these materials highly attractive for developing hydrogel scaffolds for tissue regeneration.",
publisher = "Amer Inst Physics, Melville",
journal = "VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology",
title = "2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration",
pages = "4949668",
volume = "1736",
doi = "10.1063/1.4949668"
}

Tomić, S., Babić, M., Vuković, J., Perišić, M. D., Filipović, V., Davidović, S.,& Filipović, J. M.. (2016). 2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration. in VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology
Amer Inst Physics, Melville., 1736, 4949668.
https://doi.org/10.1063/1.4949668

Tomić S, Babić M, Vuković J, Perišić MD, Filipović V, Davidović S, Filipović JM. 2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration. in VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology. 2016;1736:4949668.
doi:10.1063/1.4949668 .

Tomić, Simonida, Babić, Marija, Vuković, Jovana, Perišić, Marija D., Filipović, Vuk, Davidović, Slađana, Filipović, Jovanka M., "2-Hydroxyethyl Metahcrylate/Gelatin based Superporous Hydrogels for Tissue Regeneration" in VIII International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology, 1736 (2016):4949668,
https://doi.org/10.1063/1.4949668 . .

TY  - JOUR
AU  - Antić, Katarina
AU  - Babić, Marija
AU  - Vuković, Jovana
AU  - Onjia, Antonije
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3231
AB  - A series of poly(2-hydroxyethyl acrylate-co-itaconic acid), P(HEA/IA), hydrogels with different HEA/IA ratio, were synthesized using free radical crosslinking/copolymerization and investigated as sorbents for Pb2+ from aqueous solutions. Hydrogels were characterized using DMA, FTIR, DSC, SEM and AFM. The adsorption was found to be highly dependent on hydrogel composition, solution pH, sorbent weight, ionic strength and contact time. Five isotherm models, Langmuir, Freundlich, Redlich-Peterson, Temkin and Dubinin-Radushkevich, were applied to the sorption data. The best fit was obtained with Redlich-Peterson isotherm. The separation factor, RL, value indicated favorable sorption for Pb2+. The maximum sorption capacities were 392.2 and 409.8 mg/g for P(HEA/2IA) and P(HEA/10IA), respectively. Kinetic data showed best fit with pseudo-second-order model. Thermodynamic studies revealed that the reaction was exothermic and proceeds with a decrease in entropy. Moreover, P(HEA/IA) hydrogel showed the most pronounced sorption toward Pb2+ from environment containing Cu2+, Zn2+, Cd2+, Ni2+ and Co2+. Sorption/desorption experiments, showed that the P(HEA/IA) hydrogels could be reused without significant loss of the initial properties even after three adsorption-desorption cycles.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels
EP  - 705
IS  - 6
SP  - 695
VL  - 70
DO  - 10.2298/HEMIND151225006A
ER  - 

@article{
author = "Antić, Katarina and Babić, Marija and Vuković, Jovana and Onjia, Antonije and Filipović, Jovanka M. and Tomić, Simonida",
year = "2016",
abstract = "A series of poly(2-hydroxyethyl acrylate-co-itaconic acid), P(HEA/IA), hydrogels with different HEA/IA ratio, were synthesized using free radical crosslinking/copolymerization and investigated as sorbents for Pb2+ from aqueous solutions. Hydrogels were characterized using DMA, FTIR, DSC, SEM and AFM. The adsorption was found to be highly dependent on hydrogel composition, solution pH, sorbent weight, ionic strength and contact time. Five isotherm models, Langmuir, Freundlich, Redlich-Peterson, Temkin and Dubinin-Radushkevich, were applied to the sorption data. The best fit was obtained with Redlich-Peterson isotherm. The separation factor, RL, value indicated favorable sorption for Pb2+. The maximum sorption capacities were 392.2 and 409.8 mg/g for P(HEA/2IA) and P(HEA/10IA), respectively. Kinetic data showed best fit with pseudo-second-order model. Thermodynamic studies revealed that the reaction was exothermic and proceeds with a decrease in entropy. Moreover, P(HEA/IA) hydrogel showed the most pronounced sorption toward Pb2+ from environment containing Cu2+, Zn2+, Cd2+, Ni2+ and Co2+. Sorption/desorption experiments, showed that the P(HEA/IA) hydrogels could be reused without significant loss of the initial properties even after three adsorption-desorption cycles.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels",
pages = "705-695",
number = "6",
volume = "70",
doi = "10.2298/HEMIND151225006A"
}

Antić, K., Babić, M., Vuković, J., Onjia, A., Filipović, J. M.,& Tomić, S.. (2016). Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(6), 695-705.
https://doi.org/10.2298/HEMIND151225006A

Antić K, Babić M, Vuković J, Onjia A, Filipović JM, Tomić S. Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels. in Hemijska industrija. 2016;70(6):695-705.
doi:10.2298/HEMIND151225006A .

Antić, Katarina, Babić, Marija, Vuković, Jovana, Onjia, Antonije, Filipović, Jovanka M., Tomić, Simonida, "Removal of Pb2+ from aqueous solution by P(HEA/IA) hydrogels" in Hemijska industrija, 70, no. 6 (2016):695-705,
https://doi.org/10.2298/HEMIND151225006A . .

TY  - JOUR
AU  - Vuković, Jovana
AU  - Babić, Marija
AU  - Antić, Katarina
AU  - Filipović, Jovanka M.
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3352
AB  - In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.
PB  - Elsevier Science Sa, Lausanne
T2  - Materials Chemistry and Physics
T1  - In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management
EP  - 163
SP  - 158
VL  - 175
DO  - 10.1016/j.matchemphys.2016.03.009
ER  - 

@article{
author = "Vuković, Jovana and Babić, Marija and Antić, Katarina and Filipović, Jovanka M. and Stojanović, Sanja T. and Najman, Stevo and Tomić, Simonida",
year = "2016",
abstract = "In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Materials Chemistry and Physics",
title = "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management",
pages = "163-158",
volume = "175",
doi = "10.1016/j.matchemphys.2016.03.009"
}

Vuković, J., Babić, M., Antić, K., Filipović, J. M., Stojanović, S. T., Najman, S.,& Tomić, S.. (2016). In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics
Elsevier Science Sa, Lausanne., 175, 158-163.
https://doi.org/10.1016/j.matchemphys.2016.03.009

Vuković J, Babić M, Antić K, Filipović JM, Stojanović ST, Najman S, Tomić S. In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics. 2016;175:158-163.
doi:10.1016/j.matchemphys.2016.03.009 .

Vuković, Jovana, Babić, Marija, Antić, Katarina, Filipović, Jovanka M., Stojanović, Sanja T., Najman, Stevo, Tomić, Simonida, "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management" in Materials Chemistry and Physics, 175 (2016):158-163,
https://doi.org/10.1016/j.matchemphys.2016.03.009 . .

TY  - JOUR
AU  - Takić-Miladinov, Dijana
AU  - Tomić, Simonida
AU  - Stojanović, Sanja T.
AU  - Najdanović, Jelena
AU  - Filipović, Jovanka
AU  - Trajanović, Miroslav
AU  - Najman, Stevo
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3307
AB  - In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.
PB  - University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos
T2  - Materials Research-Ibero-American Journal of Materials
T1  - Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid
EP  - 1079
IS  - 5
SP  - 1070
VL  - 19
DO  - 10.1590/1980-5373-MR-2016-0222
ER  - 

@article{
author = "Takić-Miladinov, Dijana and Tomić, Simonida and Stojanović, Sanja T. and Najdanović, Jelena and Filipović, Jovanka and Trajanović, Miroslav and Najman, Stevo",
year = "2016",
abstract = "In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.",
publisher = "University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos",
journal = "Materials Research-Ibero-American Journal of Materials",
title = "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid",
pages = "1079-1070",
number = "5",
volume = "19",
doi = "10.1590/1980-5373-MR-2016-0222"
}

Takić-Miladinov, D., Tomić, S., Stojanović, S. T., Najdanović, J., Filipović, J., Trajanović, M.,& Najman, S.. (2016). Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials
University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos., 19(5), 1070-1079.
https://doi.org/10.1590/1980-5373-MR-2016-0222

Takić-Miladinov D, Tomić S, Stojanović ST, Najdanović J, Filipović J, Trajanović M, Najman S. Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials. 2016;19(5):1070-1079.
doi:10.1590/1980-5373-MR-2016-0222 .

Takić-Miladinov, Dijana, Tomić, Simonida, Stojanović, Sanja T., Najdanović, Jelena, Filipović, Jovanka, Trajanović, Miroslav, Najman, Stevo, "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid" in Materials Research-Ibero-American Journal of Materials, 19, no. 5 (2016):1070-1079,
https://doi.org/10.1590/1980-5373-MR-2016-0222 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Božić, Biljana
AU  - Filipović, Jovanka M.
AU  - Ušćumlić, Gordana
AU  - Tomić, Simonida
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3126
AB  - A series of dual-sensitive poly(2-hydroxyethyl acrylate/itaconic acid) (P(HEA/IA)) hydrogels were synthesized and evaluated as drug delivery systems for potential antiproliferative agents. Investigated hydrophobic compounds, Mn(II) and Zn(II) complexes with Oxaprozin, were efficiently loaded into the P(HEA/IA) hydrogels, which was confirmed by FTIR and UV-Vis spectroscopy. Swelling studies, conducted in the physiological pH range of 2.20-8.00 and in temperature range of 30-50 A degrees C, demonstrated that loaded transition metal complexes in P(HEA/IA) hydrogels did not annul pH and temperature sensitivity of the hydrogels. In vitro antiproliferative activity of Mn(II) and Zn(II) complexes with Oxaprozin against human cervical (HeLa) and melanoma cancer (Fem X) cell lines was tested. Results of in vitro release study investigated at different pH conditions confirmed P(HEA/IA) hydrogels as a highly effective pH-triggered drug delivery system for hydrophobic antiproliferative agents. These performances indicate that P(HEA/IA) hydrogels loaded with transition metal complexes can be further studied as a promising candidate for anticancer therapy, as well as for targeted treatment of intestine/colon cancers.
PB  - Springer, New York
T2  - Journal of Materials Science
T1  - Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents
EP  - 6219
IS  - 18
SP  - 6208
VL  - 50
DO  - 10.1007/s10853-015-9179-6
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Božić, Biljana and Filipović, Jovanka M. and Ušćumlić, Gordana and Tomić, Simonida",
year = "2015",
abstract = "A series of dual-sensitive poly(2-hydroxyethyl acrylate/itaconic acid) (P(HEA/IA)) hydrogels were synthesized and evaluated as drug delivery systems for potential antiproliferative agents. Investigated hydrophobic compounds, Mn(II) and Zn(II) complexes with Oxaprozin, were efficiently loaded into the P(HEA/IA) hydrogels, which was confirmed by FTIR and UV-Vis spectroscopy. Swelling studies, conducted in the physiological pH range of 2.20-8.00 and in temperature range of 30-50 A degrees C, demonstrated that loaded transition metal complexes in P(HEA/IA) hydrogels did not annul pH and temperature sensitivity of the hydrogels. In vitro antiproliferative activity of Mn(II) and Zn(II) complexes with Oxaprozin against human cervical (HeLa) and melanoma cancer (Fem X) cell lines was tested. Results of in vitro release study investigated at different pH conditions confirmed P(HEA/IA) hydrogels as a highly effective pH-triggered drug delivery system for hydrophobic antiproliferative agents. These performances indicate that P(HEA/IA) hydrogels loaded with transition metal complexes can be further studied as a promising candidate for anticancer therapy, as well as for targeted treatment of intestine/colon cancers.",
publisher = "Springer, New York",
journal = "Journal of Materials Science",
title = "Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents",
pages = "6219-6208",
number = "18",
volume = "50",
doi = "10.1007/s10853-015-9179-6"
}

Babić, M., Božić, B., Božić, B., Filipović, J. M., Ušćumlić, G.,& Tomić, S.. (2015). Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents. in Journal of Materials Science
Springer, New York., 50(18), 6208-6219.
https://doi.org/10.1007/s10853-015-9179-6

Babić M, Božić B, Božić B, Filipović JM, Ušćumlić G, Tomić S. Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents. in Journal of Materials Science. 2015;50(18):6208-6219.
doi:10.1007/s10853-015-9179-6 .

Babić, Marija, Božić, Bojan, Božić, Biljana, Filipović, Jovanka M., Ušćumlić, Gordana, Tomić, Simonida, "Evaluation of poly(hydroxyethyl acrylate/itaconic acid) hydrogels for controlled delivery of transition metal complexes with Oxaprozin as potential antiproliferative agents" in Journal of Materials Science, 50, no. 18 (2015):6208-6219,
https://doi.org/10.1007/s10853-015-9179-6 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Božić, Bojan
AU  - Antić, Katarina
AU  - Jovašević-Vuković, Jovana J.
AU  - Perišić, Marija D.
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2970
AB  - Series of novel dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels were designed as multifunctional drug delivery system which can provide several advantages including drug protection, self-regulated oscillatory release and targeted delivery to a single entity. The hydrogels were synthesized by the free-radical crosslinking copolymerization and evaluated as carriers for hydrophobic drug, Oxaprozin, with specific controlled release properties. Structural, morphological, thermal, surface charge, swelling and antimicrobial properties of the hydrogels were investigated for unloaded and Oxaprozin-loaded samples. Swelling studies demonstrated pH- and temperature-sensitivity of drug-free and drug-loaded P(HPA/IA) hydrogels. The results of swelling and oscillatory swelling, and swelling behavior of drug-free, and drug-loaded hydrogels in simulated gastrointestinal conditions, and in vitro Oxaprozin release studies confirmed these hydrogels as a highly effective colon-specific drug delivery system with excellent performance of long-term controlled release. These unique properties make the P(HPA/IA) hydrogels highly attractive materials for developing multifunctional drug delivery systems.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Letters
T1  - Design of novel multifunctional Oxaprozin delivery system based on dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels
EP  - 68
SP  - 64
VL  - 147
DO  - 10.1016/j.matlet.2015.02.035
ER  - 

@article{
author = "Babić, Marija and Božić, Bojan and Antić, Katarina and Jovašević-Vuković, Jovana J. and Perišić, Marija D. and Filipović, Jovanka M. and Tomić, Simonida",
year = "2015",
abstract = "Series of novel dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels were designed as multifunctional drug delivery system which can provide several advantages including drug protection, self-regulated oscillatory release and targeted delivery to a single entity. The hydrogels were synthesized by the free-radical crosslinking copolymerization and evaluated as carriers for hydrophobic drug, Oxaprozin, with specific controlled release properties. Structural, morphological, thermal, surface charge, swelling and antimicrobial properties of the hydrogels were investigated for unloaded and Oxaprozin-loaded samples. Swelling studies demonstrated pH- and temperature-sensitivity of drug-free and drug-loaded P(HPA/IA) hydrogels. The results of swelling and oscillatory swelling, and swelling behavior of drug-free, and drug-loaded hydrogels in simulated gastrointestinal conditions, and in vitro Oxaprozin release studies confirmed these hydrogels as a highly effective colon-specific drug delivery system with excellent performance of long-term controlled release. These unique properties make the P(HPA/IA) hydrogels highly attractive materials for developing multifunctional drug delivery systems.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Letters",
title = "Design of novel multifunctional Oxaprozin delivery system based on dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels",
pages = "68-64",
volume = "147",
doi = "10.1016/j.matlet.2015.02.035"
}

Babić, M., Božić, B., Antić, K., Jovašević-Vuković, J. J., Perišić, M. D., Filipović, J. M.,& Tomić, S.. (2015). Design of novel multifunctional Oxaprozin delivery system based on dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels. in Materials Letters
Elsevier Science Bv, Amsterdam., 147, 64-68.
https://doi.org/10.1016/j.matlet.2015.02.035

Babić M, Božić B, Antić K, Jovašević-Vuković JJ, Perišić MD, Filipović JM, Tomić S. Design of novel multifunctional Oxaprozin delivery system based on dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels. in Materials Letters. 2015;147:64-68.
doi:10.1016/j.matlet.2015.02.035 .

Babić, Marija, Božić, Bojan, Antić, Katarina, Jovašević-Vuković, Jovana J., Perišić, Marija D., Filipović, Jovanka M., Tomić, Simonida, "Design of novel multifunctional Oxaprozin delivery system based on dual-sensitive poly(2-hydroxypropyl acrylate/itaconic acid) hydrogels" in Materials Letters, 147 (2015):64-68,
https://doi.org/10.1016/j.matlet.2015.02.035 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Antić, Katarina
AU  - Jovašević-Vuković, Jovana J.
AU  - Božić, Bojan
AU  - Davidović, Slađana
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3023
AB  - In this study, a series of novel stimuli-sensitive hydrogels based on 2-hydroxyethyl acrylate and itaconic acid monomers were designed for the controlled release of hydrophobic drug, Oxaprozin. All samples were synthesized by the free-radical crosslinking copolymerization and characterized for structural, morphological, thermal, surface charge, swelling and antibacterial properties. In order to investigate the influence of the drug on hydrogel properties the same characterization was conducted for all Oxaprozin-loaded samples. The chemical composition of hydrogels was studied using Fourier transform infrared spectroscopy, while their morphology and thermal properties were examined by scanning electron microscopy and differential scanning calorimetry. Swelling studies, conducted in the physiological pH range from 2.20 to 8.00 and in the temperature range from 25 to 50 degrees C, showed that the loaded drug does not modify the pH and temperature sensitivity of the hydrogels, but reduces their swelling capacity. The in vitro drug release study conducted at pH 2.20 and 7.40 showed that all hydrogels can be tailored as colon specific drug delivery systems, and the drug release rate can be effectively controlled by IA content. In addition, the antibacterial activity of the hydrogels was determined against Escherichia coli and Staphylococcus aureus, by the zone of inhibition test. Results of our study indicate that these "smart" hydrogels, with specific morphology, surface charge, swelling capacity, drug loading efficiency and release behavior, could be designed to obtain an enhanced and site-specific controlled drug release system by simply adjusting their composition.
PB  - Springer, New York
T2  - Journal of Materials Science
T1  - Oxaprozin/poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels: morphological, thermal, swelling, drug release and antibacterial properties
EP  - 922
IS  - 2
SP  - 906
VL  - 50
DO  - 10.1007/s10853-014-8651-z
ER  - 

@article{
author = "Babić, Marija and Antić, Katarina and Jovašević-Vuković, Jovana J. and Božić, Bojan and Davidović, Slađana and Filipović, Jovanka M. and Tomić, Simonida",
year = "2015",
abstract = "In this study, a series of novel stimuli-sensitive hydrogels based on 2-hydroxyethyl acrylate and itaconic acid monomers were designed for the controlled release of hydrophobic drug, Oxaprozin. All samples were synthesized by the free-radical crosslinking copolymerization and characterized for structural, morphological, thermal, surface charge, swelling and antibacterial properties. In order to investigate the influence of the drug on hydrogel properties the same characterization was conducted for all Oxaprozin-loaded samples. The chemical composition of hydrogels was studied using Fourier transform infrared spectroscopy, while their morphology and thermal properties were examined by scanning electron microscopy and differential scanning calorimetry. Swelling studies, conducted in the physiological pH range from 2.20 to 8.00 and in the temperature range from 25 to 50 degrees C, showed that the loaded drug does not modify the pH and temperature sensitivity of the hydrogels, but reduces their swelling capacity. The in vitro drug release study conducted at pH 2.20 and 7.40 showed that all hydrogels can be tailored as colon specific drug delivery systems, and the drug release rate can be effectively controlled by IA content. In addition, the antibacterial activity of the hydrogels was determined against Escherichia coli and Staphylococcus aureus, by the zone of inhibition test. Results of our study indicate that these "smart" hydrogels, with specific morphology, surface charge, swelling capacity, drug loading efficiency and release behavior, could be designed to obtain an enhanced and site-specific controlled drug release system by simply adjusting their composition.",
publisher = "Springer, New York",
journal = "Journal of Materials Science",
title = "Oxaprozin/poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels: morphological, thermal, swelling, drug release and antibacterial properties",
pages = "922-906",
number = "2",
volume = "50",
doi = "10.1007/s10853-014-8651-z"
}

Babić, M., Antić, K., Jovašević-Vuković, J. J., Božić, B., Davidović, S., Filipović, J. M.,& Tomić, S.. (2015). Oxaprozin/poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels: morphological, thermal, swelling, drug release and antibacterial properties. in Journal of Materials Science
Springer, New York., 50(2), 906-922.
https://doi.org/10.1007/s10853-014-8651-z

Babić M, Antić K, Jovašević-Vuković JJ, Božić B, Davidović S, Filipović JM, Tomić S. Oxaprozin/poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels: morphological, thermal, swelling, drug release and antibacterial properties. in Journal of Materials Science. 2015;50(2):906-922.
doi:10.1007/s10853-014-8651-z .

Babić, Marija, Antić, Katarina, Jovašević-Vuković, Jovana J., Božić, Bojan, Davidović, Slađana, Filipović, Jovanka M., Tomić, Simonida, "Oxaprozin/poly(2-hydroxyethyl acrylate/itaconic acid) hydrogels: morphological, thermal, swelling, drug release and antibacterial properties" in Journal of Materials Science, 50, no. 2 (2015):906-922,
https://doi.org/10.1007/s10853-014-8651-z . .

TY  - JOUR
AU  - Antić, Katarina
AU  - Babić, Marija
AU  - Jovašević-Vuković, Jovana J.
AU  - Vasiljević-Radović, Dana
AU  - Onjia, Antonije
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3027
AB  - Series of novel hydrogels based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), P(HEA/IA) copolymers, were prepared by free radical cross-linking copolymerization and investigated as potential adsorbents for Cd2+ removal from aqueous solution. The hydrogels before and after Cd2+ adsorption were characterized using FTIR, temperature sensitive. In order to evaluate adsorption behavior of samples various factors affecting the Cd2+ uptake behavior, such as: contact time, temperature, pH, ionic strength, adsorbent weight, competitive ions and initial concentration of the metal ions were investigated. Five adsorption isotherms and two kinetic models were studied. The adsorption behavior can be very well described by the pseudo-second order kinetic model and Langmuir isotherm. Multicomponent adsorption studies revealed that adsorption of cadmium depends on the type of metal ions present in the system. Desorption studies showed that hydrogel can be reused three times with only 15% loss of adsorption capacity. All results indicate that the sample with the highest IA content is the most promising adsorbent for Cd2+ removal.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Surface Science
T1  - Preparation and characterization of novel P(HEA/IA) hydrogels for Cd2+ ion removal from aqueous solution
EP  - 189
SP  - 178
VL  - 338
DO  - 10.1016/j.apsusc.2015.02.133
ER  - 

@article{
author = "Antić, Katarina and Babić, Marija and Jovašević-Vuković, Jovana J. and Vasiljević-Radović, Dana and Onjia, Antonije and Filipović, Jovanka M. and Tomić, Simonida",
year = "2015",
abstract = "Series of novel hydrogels based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), P(HEA/IA) copolymers, were prepared by free radical cross-linking copolymerization and investigated as potential adsorbents for Cd2+ removal from aqueous solution. The hydrogels before and after Cd2+ adsorption were characterized using FTIR, temperature sensitive. In order to evaluate adsorption behavior of samples various factors affecting the Cd2+ uptake behavior, such as: contact time, temperature, pH, ionic strength, adsorbent weight, competitive ions and initial concentration of the metal ions were investigated. Five adsorption isotherms and two kinetic models were studied. The adsorption behavior can be very well described by the pseudo-second order kinetic model and Langmuir isotherm. Multicomponent adsorption studies revealed that adsorption of cadmium depends on the type of metal ions present in the system. Desorption studies showed that hydrogel can be reused three times with only 15% loss of adsorption capacity. All results indicate that the sample with the highest IA content is the most promising adsorbent for Cd2+ removal.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Surface Science",
title = "Preparation and characterization of novel P(HEA/IA) hydrogels for Cd2+ ion removal from aqueous solution",
pages = "189-178",
volume = "338",
doi = "10.1016/j.apsusc.2015.02.133"
}

Antić, K., Babić, M., Jovašević-Vuković, J. J., Vasiljević-Radović, D., Onjia, A., Filipović, J. M.,& Tomić, S.. (2015). Preparation and characterization of novel P(HEA/IA) hydrogels for Cd2+ ion removal from aqueous solution. in Applied Surface Science
Elsevier Science Bv, Amsterdam., 338, 178-189.
https://doi.org/10.1016/j.apsusc.2015.02.133

Antić K, Babić M, Jovašević-Vuković JJ, Vasiljević-Radović D, Onjia A, Filipović JM, Tomić S. Preparation and characterization of novel P(HEA/IA) hydrogels for Cd2+ ion removal from aqueous solution. in Applied Surface Science. 2015;338:178-189.
doi:10.1016/j.apsusc.2015.02.133 .

Antić, Katarina, Babić, Marija, Jovašević-Vuković, Jovana J., Vasiljević-Radović, Dana, Onjia, Antonije, Filipović, Jovanka M., Tomić, Simonida, "Preparation and characterization of novel P(HEA/IA) hydrogels for Cd2+ ion removal from aqueous solution" in Applied Surface Science, 338 (2015):178-189,
https://doi.org/10.1016/j.apsusc.2015.02.133 . .

TY  - JOUR
AU  - Vuković, Jovana
AU  - Babić, Marija
AU  - Antić, Katarina
AU  - Miljković, Miona
AU  - Perić-Grujić, Aleksandra
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3139
AB  - In this study, three series of hydrogels based on 2-hydroxyethyl acrylate and itaconic acid, unloaded, with incorporated copper(II) ions and reduced copper, were successfully prepared, characterized and evaluated as novel wound healing materials. Fourier transform infrared spectroscopy (FTIR) confirmed the expected structure of obtained hydrogels. Scanning electron microscopy (SEM) revealed porous morphology of unloaded hydrogels, and the morphological modifications in case of loaded hydrogels. Thermal characteristics were examined by differential scanning calorimetry (DSC) and the glass transition temperatures were observed in range of 12-50 degrees C. Swelling study was conducted in wide range of pHs at 37 degrees C, confirming pH sensitive behaviour for all three series of hydrogels. The in vitro copper release was investigated and the experimental data were analysed using several models in order to elucidate the transport mechanism. The antimicrobial assay revealed excellent antimicrobial activity, over 99% against Escherichia coli, Staphylococcus aureus and Candida albicans, as well as good correlation with the copper release experiments. In accordance with potential application, water vapour transmission rate, oxygen penetration, dispersion characteristics, fluid retention were observed and the suitability of the hydrogels for wound healing application was discussed.
PB  - Elsevier Science Sa, Lausanne
T2  - Materials Chemistry and Physics
T1  - A high efficacy antimicrobial acrylate based hydrogels with incorporated copper for wound healing application
EP  - 62
SP  - 51
VL  - 164
DO  - 10.1016/j.matchemphys.2015.08.022
ER  - 

@article{
author = "Vuković, Jovana and Babić, Marija and Antić, Katarina and Miljković, Miona and Perić-Grujić, Aleksandra and Filipović, Jovanka M. and Tomić, Simonida",
year = "2015",
abstract = "In this study, three series of hydrogels based on 2-hydroxyethyl acrylate and itaconic acid, unloaded, with incorporated copper(II) ions and reduced copper, were successfully prepared, characterized and evaluated as novel wound healing materials. Fourier transform infrared spectroscopy (FTIR) confirmed the expected structure of obtained hydrogels. Scanning electron microscopy (SEM) revealed porous morphology of unloaded hydrogels, and the morphological modifications in case of loaded hydrogels. Thermal characteristics were examined by differential scanning calorimetry (DSC) and the glass transition temperatures were observed in range of 12-50 degrees C. Swelling study was conducted in wide range of pHs at 37 degrees C, confirming pH sensitive behaviour for all three series of hydrogels. The in vitro copper release was investigated and the experimental data were analysed using several models in order to elucidate the transport mechanism. The antimicrobial assay revealed excellent antimicrobial activity, over 99% against Escherichia coli, Staphylococcus aureus and Candida albicans, as well as good correlation with the copper release experiments. In accordance with potential application, water vapour transmission rate, oxygen penetration, dispersion characteristics, fluid retention were observed and the suitability of the hydrogels for wound healing application was discussed.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Materials Chemistry and Physics",
title = "A high efficacy antimicrobial acrylate based hydrogels with incorporated copper for wound healing application",
pages = "62-51",
volume = "164",
doi = "10.1016/j.matchemphys.2015.08.022"
}

Vuković, J., Babić, M., Antić, K., Miljković, M., Perić-Grujić, A., Filipović, J. M.,& Tomić, S.. (2015). A high efficacy antimicrobial acrylate based hydrogels with incorporated copper for wound healing application. in Materials Chemistry and Physics
Elsevier Science Sa, Lausanne., 164, 51-62.
https://doi.org/10.1016/j.matchemphys.2015.08.022

Vuković J, Babić M, Antić K, Miljković M, Perić-Grujić A, Filipović JM, Tomić S. A high efficacy antimicrobial acrylate based hydrogels with incorporated copper for wound healing application. in Materials Chemistry and Physics. 2015;164:51-62.
doi:10.1016/j.matchemphys.2015.08.022 .

Vuković, Jovana, Babić, Marija, Antić, Katarina, Miljković, Miona, Perić-Grujić, Aleksandra, Filipović, Jovanka M., Tomić, Simonida, "A high efficacy antimicrobial acrylate based hydrogels with incorporated copper for wound healing application" in Materials Chemistry and Physics, 164 (2015):51-62,
https://doi.org/10.1016/j.matchemphys.2015.08.022 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lučić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2771
AB  - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Hindawi Publishing Corp, New York
T2  - Scientific World Journal
T1  - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
DO  - 10.1155/2014/142123
ER  - 

@article{
author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lučić, Marija and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2014",
abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Scientific World Journal",
title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel",
doi = "10.1155/2014/142123"
}

Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lučić, M., Filipović, J.,& Kalagasidis Krušić, M.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/142123

Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lučić M, Filipović J, Kalagasidis Krušić M. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal. 2014;.
doi:10.1155/2014/142123 .

Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/142123 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lučić-Škorić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2828
AB  - Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
VL  - 2014
DO  - 10.1155/2014/364930
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lučić-Škorić, Marija and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2014",
abstract = "Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions",
volume = "2014",
doi = "10.1155/2014/364930"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Lučić-Škorić, M., Filipović, J.,& Kalagasidis Krušić, M.. (2014). Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed Research International
Hindawi Ltd, London., 2014.
https://doi.org/10.1155/2014/364930

Milašinović N, Knežević-Jugović Z, Milosavljević N, Lučić-Škorić M, Filipović J, Kalagasidis Krušić M. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed Research International. 2014;2014.
doi:10.1155/2014/364930 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić-Škorić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions" in Biomed Research International, 2014 (2014),
https://doi.org/10.1155/2014/364930 . .

TY  - JOUR
AU  - Malešić, Neda B.
AU  - Rusmirović, Jelena
AU  - Jovašević, Jovana S.
AU  - Perišić, Marija D.
AU  - Dimitrijević, Suzana
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2595
AB  - The objective of our study was to develop new antimicrobial hydrogels silver/poly(2-hydroxyethyl methacrylate/ itaconic acid) (Ag/P(HEMA/IA)). The P(HEMA/IA) samples, with different HEMA/IA ratio, were prepared by free radical crosslinking copolymerization. Ag ion were introduced in hydrogels by immersing dried P(HEMA/IA) disks in silver(I) salt solution, which was confirmed by FTIR spectroscopy. The in vitro controlled release of silver(I) ion from (Ag/P(HEMA/IA)) gels, and antimicrobial activity during the release period were also studied. The release profiles have shown a two-phase exponential profile, with fast initial phase, followed by a much slower release rate. Kinetic parameters determined, have indicated Fickian transport of Ag(I) ion in the initial phase. It is perceived that the antimicrobial activity of the Ag/P(HEMA/IA) depends on IA content. Excellent antimicrobial potential was maintained during the entire release time.
AB  - Cilj našeg istraživanja je bio da se razviju novi antimikrobni hidrogelovi. Srebro/poli(2-hidroksietil metakrilat/itakonska kiselina) (Ag/P(HEMA/IK)) hidrogelovi su sintetisani, i proučavano je kontrolisano otpuštanje srebro(I) jona iz Ag/P(HEMA/IK) hidrogelova, kao i njihov antimikrobni potencijal. P(HEMA/IK) uzorci, sa različitim odnosima monomera HEMA/IK, dobijeni su radikalnom kopolimerizacijom/ umrežavanjem. Ag/P(HEMA/IK) hidrogelovi su formirani potapanjem suvih diskova gelova u srebro(I) so, što je i potvrđeno FTIR spektroskopijom. Ispitani su kontrolisano otpuštanje srebro(I) jona iz Ag/P(HEMA/IA) gelova in vitro, kao i antimikrobna aktivnost tokom perioda otpuštanja. Profili otpuštanja su pokazali dve faze (eksponencijalni rast), sa brzom početnom fazom, a zatim sporijom brzinom otpuštanja. Antimikrobna aktivnost u toku otpuštanja u maloj meri zavisi od sadržaja IA i vremena otpuštanja. Odličan antimikrobni potencijal se održava tokom celog vremena otpuštanja. Na osnovu dobijenih rezultata proizilazi da se Ag/P(HEMA/IK) hidrogelovi mogu koristiti u širokom spektru biomedicinskih primena.
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika
T1  - Antimicrobial hydrogels based on 2-hydroxyethyl methacrylate and itaconic acid containing silver(I) ion
T1  - Antimikrobni hidrogelovi na bazi 2-hidroksietil metakrilata i itakonske kiseline koji sadrže srebro(I) jon
EP  - 568
IS  - 4
SP  - 563
VL  - 69
DO  - 10.5937/tehnika1404563M
ER  - 

@article{
author = "Malešić, Neda B. and Rusmirović, Jelena and Jovašević, Jovana S. and Perišić, Marija D. and Dimitrijević, Suzana and Filipović, Jovanka M. and Tomić, Simonida",
year = "2014",
abstract = "The objective of our study was to develop new antimicrobial hydrogels silver/poly(2-hydroxyethyl methacrylate/ itaconic acid) (Ag/P(HEMA/IA)). The P(HEMA/IA) samples, with different HEMA/IA ratio, were prepared by free radical crosslinking copolymerization. Ag ion were introduced in hydrogels by immersing dried P(HEMA/IA) disks in silver(I) salt solution, which was confirmed by FTIR spectroscopy. The in vitro controlled release of silver(I) ion from (Ag/P(HEMA/IA)) gels, and antimicrobial activity during the release period were also studied. The release profiles have shown a two-phase exponential profile, with fast initial phase, followed by a much slower release rate. Kinetic parameters determined, have indicated Fickian transport of Ag(I) ion in the initial phase. It is perceived that the antimicrobial activity of the Ag/P(HEMA/IA) depends on IA content. Excellent antimicrobial potential was maintained during the entire release time., Cilj našeg istraživanja je bio da se razviju novi antimikrobni hidrogelovi. Srebro/poli(2-hidroksietil metakrilat/itakonska kiselina) (Ag/P(HEMA/IK)) hidrogelovi su sintetisani, i proučavano je kontrolisano otpuštanje srebro(I) jona iz Ag/P(HEMA/IK) hidrogelova, kao i njihov antimikrobni potencijal. P(HEMA/IK) uzorci, sa različitim odnosima monomera HEMA/IK, dobijeni su radikalnom kopolimerizacijom/ umrežavanjem. Ag/P(HEMA/IK) hidrogelovi su formirani potapanjem suvih diskova gelova u srebro(I) so, što je i potvrđeno FTIR spektroskopijom. Ispitani su kontrolisano otpuštanje srebro(I) jona iz Ag/P(HEMA/IA) gelova in vitro, kao i antimikrobna aktivnost tokom perioda otpuštanja. Profili otpuštanja su pokazali dve faze (eksponencijalni rast), sa brzom početnom fazom, a zatim sporijom brzinom otpuštanja. Antimikrobna aktivnost u toku otpuštanja u maloj meri zavisi od sadržaja IA i vremena otpuštanja. Odličan antimikrobni potencijal se održava tokom celog vremena otpuštanja. Na osnovu dobijenih rezultata proizilazi da se Ag/P(HEMA/IK) hidrogelovi mogu koristiti u širokom spektru biomedicinskih primena.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika",
title = "Antimicrobial hydrogels based on 2-hydroxyethyl methacrylate and itaconic acid containing silver(I) ion, Antimikrobni hidrogelovi na bazi 2-hidroksietil metakrilata i itakonske kiseline koji sadrže srebro(I) jon",
pages = "568-563",
number = "4",
volume = "69",
doi = "10.5937/tehnika1404563M"
}

Malešić, N. B., Rusmirović, J., Jovašević, J. S., Perišić, M. D., Dimitrijević, S., Filipović, J. M.,& Tomić, S.. (2014). Antimicrobial hydrogels based on 2-hydroxyethyl methacrylate and itaconic acid containing silver(I) ion. in Tehnika
Savez inženjera i tehničara Srbije, Beograd., 69(4), 563-568.
https://doi.org/10.5937/tehnika1404563M

Malešić NB, Rusmirović J, Jovašević JS, Perišić MD, Dimitrijević S, Filipović JM, Tomić S. Antimicrobial hydrogels based on 2-hydroxyethyl methacrylate and itaconic acid containing silver(I) ion. in Tehnika. 2014;69(4):563-568.
doi:10.5937/tehnika1404563M .

Malešić, Neda B., Rusmirović, Jelena, Jovašević, Jovana S., Perišić, Marija D., Dimitrijević, Suzana, Filipović, Jovanka M., Tomić, Simonida, "Antimicrobial hydrogels based on 2-hydroxyethyl methacrylate and itaconic acid containing silver(I) ion" in Tehnika, 69, no. 4 (2014):563-568,
https://doi.org/10.5937/tehnika1404563M . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Babić, Marija
AU  - Antić, Katarina
AU  - Jovašević-Vuković, Jovana J.
AU  - Malešić, Neda B.
AU  - Filipović, Jovanka M.
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2719
AB  - Novel hydrogels based on 2-hydroxyethyl methacrylate (HEMA), itaconic acid (IA) and different poly (alkylene glycol) (meth)acrylates (PAGM) (P(HEMA/IA/PAGM)) were synthesized. We investigated the influence of different PAGM components, with acrylic or methacrylic acid residues in the main chain and ethylene glycol (EG) and/or propylene glycol (PG) units in pendant chains of varying length, on the nature and inherent properties of P(HEMA/IA/PAGM) copolymeric hydrogels. Swelling studies revealed pH sensitive behavior of P(HEMA/IA/PAGM) samples. Hydrogel structure and morphology were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM), which confirmed their chemical structure and differences in pore size. The shear modulus values for P(HEMA/IA/PAGM) hydrogels were close to that of PHEMA, but slightly lower than the value for P(HEMA/IA). Cephalexin (CEX) drug release profiles from P(HEMA/IA/PAGM) samples showed a marked dependence on the PAGM component. The presence of IA also influenced the release rate of CEX, leading to a faster release when IA was combined with the more hydrophilic PAGM component. An in vitro assay of P(HEMA/IA/PAGM) cytotoxicity showed good cell viability. The results obtained indicate that P(HEMA/IA/PAGM) hydrogel properties were significantly dependent on the PAGM component, meaning that the type of side chains can be used to tune the characteristics of such biomaterials. These properties make P(HEMA/IA/PAGM) copolymeric hydrogels applicable in biomedical and biotechnological fields and controlled drug delivery.
PB  - Polymer Soc Korea, Seoul
T2  - Macromolecular Research
T1  - pH-Sensitive Hydrogels Based on (Meth)Acrylates and Itaconic Acid
EP  - 1213
IS  - 11
SP  - 1203
VL  - 22
DO  - 10.1007/s13233-014-2172-0
ER  - 

@article{
author = "Tomić, Simonida and Babić, Marija and Antić, Katarina and Jovašević-Vuković, Jovana J. and Malešić, Neda B. and Filipović, Jovanka M.",
year = "2014",
abstract = "Novel hydrogels based on 2-hydroxyethyl methacrylate (HEMA), itaconic acid (IA) and different poly (alkylene glycol) (meth)acrylates (PAGM) (P(HEMA/IA/PAGM)) were synthesized. We investigated the influence of different PAGM components, with acrylic or methacrylic acid residues in the main chain and ethylene glycol (EG) and/or propylene glycol (PG) units in pendant chains of varying length, on the nature and inherent properties of P(HEMA/IA/PAGM) copolymeric hydrogels. Swelling studies revealed pH sensitive behavior of P(HEMA/IA/PAGM) samples. Hydrogel structure and morphology were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM), which confirmed their chemical structure and differences in pore size. The shear modulus values for P(HEMA/IA/PAGM) hydrogels were close to that of PHEMA, but slightly lower than the value for P(HEMA/IA). Cephalexin (CEX) drug release profiles from P(HEMA/IA/PAGM) samples showed a marked dependence on the PAGM component. The presence of IA also influenced the release rate of CEX, leading to a faster release when IA was combined with the more hydrophilic PAGM component. An in vitro assay of P(HEMA/IA/PAGM) cytotoxicity showed good cell viability. The results obtained indicate that P(HEMA/IA/PAGM) hydrogel properties were significantly dependent on the PAGM component, meaning that the type of side chains can be used to tune the characteristics of such biomaterials. These properties make P(HEMA/IA/PAGM) copolymeric hydrogels applicable in biomedical and biotechnological fields and controlled drug delivery.",
publisher = "Polymer Soc Korea, Seoul",
journal = "Macromolecular Research",
title = "pH-Sensitive Hydrogels Based on (Meth)Acrylates and Itaconic Acid",
pages = "1213-1203",
number = "11",
volume = "22",
doi = "10.1007/s13233-014-2172-0"
}

Tomić, S., Babić, M., Antić, K., Jovašević-Vuković, J. J., Malešić, N. B.,& Filipović, J. M.. (2014). pH-Sensitive Hydrogels Based on (Meth)Acrylates and Itaconic Acid. in Macromolecular Research
Polymer Soc Korea, Seoul., 22(11), 1203-1213.
https://doi.org/10.1007/s13233-014-2172-0

Tomić S, Babić M, Antić K, Jovašević-Vuković JJ, Malešić NB, Filipović JM. pH-Sensitive Hydrogels Based on (Meth)Acrylates and Itaconic Acid. in Macromolecular Research. 2014;22(11):1203-1213.
doi:10.1007/s13233-014-2172-0 .

Tomić, Simonida, Babić, Marija, Antić, Katarina, Jovašević-Vuković, Jovana J., Malešić, Neda B., Filipović, Jovanka M., "pH-Sensitive Hydrogels Based on (Meth)Acrylates and Itaconic Acid" in Macromolecular Research, 22, no. 11 (2014):1203-1213,
https://doi.org/10.1007/s13233-014-2172-0 . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Jovašević, Jovana S.
AU  - Filipović, Jovanka M.
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2399
AB  - Two series of novel hydrogels, based on 2-hydroxyethyl acrylate (HEA), itaconic acid (IA), and two poly(ethylene glycol) dimethacrylates (PEGDMA), of different ethylene glycol chain lengths, were prepared by free radical crosslinking copolymerization. The influence of different ethylene glycol chain lengths and concentration in P(HEA/IA/PEGDMA) hydrogels on biocompatibility, swelling and thermal properties was investigated. All samples in contact with blood showed a mean hemolysis value  lt  1.0 % in the direct contact assay, and even  lt  0.5 % in the indirect contact assay, for in vitro testing conditions. Swelling studies, conducted in a physiological pH and temperature range, showed pH sensitivity and relatively small changes of equilibrium swelling with temperature, which varied with PEGDMA molecular weight. The glass transition temperatures (T (g)) of P(HEA/IA/PEGDMA) networks were in the range 28.1-36.9 A degrees C, respectively, and also dependent on copolymer composition. Due to good biocompatibility, favorable swelling, and thermal properties these hydrogels show good potential for biomedical uses.
PB  - Springer, New York
T2  - Polymer Bulletin
T1  - Hemocompatibility, swelling and thermal properties of hydrogels based on 2-hydroxyethyl acrylate, itaconic acid and poly(ethylene glycol) dimethacrylate
EP  - 2909
IS  - 10
SP  - 2895
VL  - 70
DO  - 10.1007/s00289-013-0995-z
ER  - 

@article{
author = "Tomić, Simonida and Jovašević, Jovana S. and Filipović, Jovanka M.",
year = "2013",
abstract = "Two series of novel hydrogels, based on 2-hydroxyethyl acrylate (HEA), itaconic acid (IA), and two poly(ethylene glycol) dimethacrylates (PEGDMA), of different ethylene glycol chain lengths, were prepared by free radical crosslinking copolymerization. The influence of different ethylene glycol chain lengths and concentration in P(HEA/IA/PEGDMA) hydrogels on biocompatibility, swelling and thermal properties was investigated. All samples in contact with blood showed a mean hemolysis value  lt  1.0 % in the direct contact assay, and even  lt  0.5 % in the indirect contact assay, for in vitro testing conditions. Swelling studies, conducted in a physiological pH and temperature range, showed pH sensitivity and relatively small changes of equilibrium swelling with temperature, which varied with PEGDMA molecular weight. The glass transition temperatures (T (g)) of P(HEA/IA/PEGDMA) networks were in the range 28.1-36.9 A degrees C, respectively, and also dependent on copolymer composition. Due to good biocompatibility, favorable swelling, and thermal properties these hydrogels show good potential for biomedical uses.",
publisher = "Springer, New York",
journal = "Polymer Bulletin",
title = "Hemocompatibility, swelling and thermal properties of hydrogels based on 2-hydroxyethyl acrylate, itaconic acid and poly(ethylene glycol) dimethacrylate",
pages = "2909-2895",
number = "10",
volume = "70",
doi = "10.1007/s00289-013-0995-z"
}

Tomić, S., Jovašević, J. S.,& Filipović, J. M.. (2013). Hemocompatibility, swelling and thermal properties of hydrogels based on 2-hydroxyethyl acrylate, itaconic acid and poly(ethylene glycol) dimethacrylate. in Polymer Bulletin
Springer, New York., 70(10), 2895-2909.
https://doi.org/10.1007/s00289-013-0995-z

Tomić S, Jovašević JS, Filipović JM. Hemocompatibility, swelling and thermal properties of hydrogels based on 2-hydroxyethyl acrylate, itaconic acid and poly(ethylene glycol) dimethacrylate. in Polymer Bulletin. 2013;70(10):2895-2909.
doi:10.1007/s00289-013-0995-z .

Tomić, Simonida, Jovašević, Jovana S., Filipović, Jovanka M., "Hemocompatibility, swelling and thermal properties of hydrogels based on 2-hydroxyethyl acrylate, itaconic acid and poly(ethylene glycol) dimethacrylate" in Polymer Bulletin, 70, no. 10 (2013):2895-2909,
https://doi.org/10.1007/s00289-013-0995-z . .

TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Dimitrijević, Suzana
AU  - Filipović, Jovanka M.
AU  - Nikolić, Rajko R.
AU  - Tomić, Simonida
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2543
AB  - New semi-interpenetrating networks (semi-IPNHs) (P(HEMA/IA)/PVP) were prepared by free radical crosslinking copolymerization of 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA, 5 mol%), in the presence of poly(vinyl pyrrolidone) (PVP, 2, 5, and 10 mol%), as an interpenetrating agent. The structure of the semi-IPNHs was confirmed by Fourier transform infrared spectroscopy, and morphology study was performed by scanning electron microscopy, which revealed the characteristic porous morphology. The results obtained by dynamic mechanical analysis showed the improvement of mechanical properties with increasing PVP content in semi-IPNs. The maximum swelling was observed for all studied systems at a slightly acidic media (around pH 6), so it can be said that the content of PVP has no influence on the swelling behavior, in the PVP range investigated. Along with the pH sensitivity, which was expected due to the presence of IA, semi-IPNHs showed temperature-sensitive swelling properties, with the lower critical solution temperature value around 41 degrees C, which is in the physiologically interesting interval. The antimicrobial activity of the samples was tested using E. coli, S. aureus, and C. albicans pathogens. It was noticed that the antimicrobial potential depends on type of microbes, time of exposure, and PVP content in the samples. Due to their good antimicrobial and mechanical properties these stimuli-sensitive semi-IPNHs have potential to be used as biomaterials for the applications in medicine and pharmacy.
PB  - Springer, New York
T2  - Polymer Bulletin
T1  - Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels
EP  - 819
IS  - 3
SP  - 809
VL  - 70
DO  - 10.1007/s00289-012-0830-y
ER  - 

@article{
author = "Krezović, Bojana D. and Dimitrijević, Suzana and Filipović, Jovanka M. and Nikolić, Rajko R. and Tomić, Simonida",
year = "2013",
abstract = "New semi-interpenetrating networks (semi-IPNHs) (P(HEMA/IA)/PVP) were prepared by free radical crosslinking copolymerization of 2-hydroxyethyl methacrylate (HEMA) and itaconic acid (IA, 5 mol%), in the presence of poly(vinyl pyrrolidone) (PVP, 2, 5, and 10 mol%), as an interpenetrating agent. The structure of the semi-IPNHs was confirmed by Fourier transform infrared spectroscopy, and morphology study was performed by scanning electron microscopy, which revealed the characteristic porous morphology. The results obtained by dynamic mechanical analysis showed the improvement of mechanical properties with increasing PVP content in semi-IPNs. The maximum swelling was observed for all studied systems at a slightly acidic media (around pH 6), so it can be said that the content of PVP has no influence on the swelling behavior, in the PVP range investigated. Along with the pH sensitivity, which was expected due to the presence of IA, semi-IPNHs showed temperature-sensitive swelling properties, with the lower critical solution temperature value around 41 degrees C, which is in the physiologically interesting interval. The antimicrobial activity of the samples was tested using E. coli, S. aureus, and C. albicans pathogens. It was noticed that the antimicrobial potential depends on type of microbes, time of exposure, and PVP content in the samples. Due to their good antimicrobial and mechanical properties these stimuli-sensitive semi-IPNHs have potential to be used as biomaterials for the applications in medicine and pharmacy.",
publisher = "Springer, New York",
journal = "Polymer Bulletin",
title = "Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels",
pages = "819-809",
number = "3",
volume = "70",
doi = "10.1007/s00289-012-0830-y"
}

Krezović, B. D., Dimitrijević, S., Filipović, J. M., Nikolić, R. R.,& Tomić, S.. (2013). Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels. in Polymer Bulletin
Springer, New York., 70(3), 809-819.
https://doi.org/10.1007/s00289-012-0830-y

Krezović BD, Dimitrijević S, Filipović JM, Nikolić RR, Tomić S. Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels. in Polymer Bulletin. 2013;70(3):809-819.
doi:10.1007/s00289-012-0830-y .

Krezović, Bojana D., Dimitrijević, Suzana, Filipović, Jovanka M., Nikolić, Rajko R., Tomić, Simonida, "Antimicrobial P(HEMA/IA)/PVP semi-interpenetrating network hydrogels" in Polymer Bulletin, 70, no. 3 (2013):809-819,
https://doi.org/10.1007/s00289-012-0830-y . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Jakovljević, Živana
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2235
AB  - A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester  gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Elsevier Science Sa, Lausanne
T2  - Chemical Engineering Journal
T1  - Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels
EP  - 623
SP  - 614
VL  - 181
DO  - 10.1016/j.cej.2011.11.115
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Jakovljević, Živana and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester  gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Chemical Engineering Journal",
title = "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels",
pages = "623-614",
volume = "181",
doi = "10.1016/j.cej.2011.11.115"
}

Milašinović, N., Knežević-Jugović, Z., Jakovljević, Ž., Filipović, J.,& Kalagasidis Krušić, M.. (2012). Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal
Elsevier Science Sa, Lausanne., 181, 614-623.
https://doi.org/10.1016/j.cej.2011.11.115

Milašinović N, Knežević-Jugović Z, Jakovljević Ž, Filipović J, Kalagasidis Krušić M. Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal. 2012;181:614-623.
doi:10.1016/j.cej.2011.11.115 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Jakovljević, Živana, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels" in Chemical Engineering Journal, 181 (2012):614-623,
https://doi.org/10.1016/j.cej.2011.11.115 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2170
AB  - The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid
EP  - 340
IS  - 1-2
SP  - 332
VL  - 436
DO  - 10.1016/j.ijpharm.2012.06.050
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid",
pages = "340-332",
number = "1-2",
volume = "436",
doi = "10.1016/j.ijpharm.2012.06.050"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Filipović, J.,& Kalagasidis Krušić, M.. (2012). Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics
Elsevier Science Bv, Amsterdam., 436(1-2), 332-340.
https://doi.org/10.1016/j.ijpharm.2012.06.050

Milašinović N, Knežević-Jugović Z, Milosavljević N, Filipović J, Kalagasidis Krušić M. Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics. 2012;436(1-2):332-340.
doi:10.1016/j.ijpharm.2012.06.050 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid" in International Journal of Pharmaceutics, 436, no. 1-2 (2012):332-340,
https://doi.org/10.1016/j.ijpharm.2012.06.050 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Milosavljević, Nedeljko
AU  - Filipović, Jovanka
AU  - Knežević-Jugović, Zorica
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2132
AB  - Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.
PB  - Springer, New York
T2  - Polymer Bulletin
T1  - Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions
EP  - 361
IS  - 3
SP  - 347
VL  - 69
DO  - 10.1007/s00289-012-0737-7
ER  - 

@article{
author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.",
publisher = "Springer, New York",
journal = "Polymer Bulletin",
title = "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions",
pages = "361-347",
number = "3",
volume = "69",
doi = "10.1007/s00289-012-0737-7"
}

Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2012). Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin
Springer, New York., 69(3), 347-361.
https://doi.org/10.1007/s00289-012-0737-7

Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin. 2012;69(3):347-361.
doi:10.1007/s00289-012-0737-7 .

Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions" in Polymer Bulletin, 69, no. 3 (2012):347-361,
https://doi.org/10.1007/s00289-012-0737-7 . .

TY  - JOUR
AU  - Babić, Marija
AU  - Jovašević, Jovana S.
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2093
AB  - The aim of this paper is to propose equations for the diffusion of drugs for investigated drug/hydrogel systems using the parameters affecting the transport of drug through poly- (2-hydroxyethylmethacrylate/itaconic acid) (P(HEMA/IA)), poly(2-hydroxyethylacrylate/ita- conic acid) (P(HEA/IA)), and poly(2-hydroxyethylmethacrylate/poly(alkyleneglycol) (meth)- acrylates) (P(HEMA/BIS)) copolymeric hydrogels. Different monomer types, as well as the variable content of some components in hydrogel composition (the amount of ionizable comonomer (IA) and different type of nonionic poly(alkyleneglycol) (meth)acrylates), ultimately defined the pore size available for drug diffusion. The hydrogels synthesized ranged from nonporous to microporous, based on the classification in accordance to the pore size, and could be classified as hydrogels that contain ionic groups and hydrogels without ionic groups. The drugs selected for this study are bronchodilators-theophylline (TPH), fenethylline hydrochloride (FE), and antibiotic cephalexin (CEX). Results of in vitro drug release tests defined the release systems based on the drug type, as well as the type of hydrogel used. The diffusion coefficient of drugs and the restriction coefficient, λ, defined as the ratio of solute to 'pore' radius (rs/rζ) that describes the ease of drug release from the gels, were used as factors that govern the release process.
AB  - Cilj ove studije je da se predlože difuzione jednačine za ispitivane sisteme lek/hidrogel. Korišćeni su hidrogelovi poli(2-hidroksietilmetakrilat/itakonska kiselina) (P(HEMA/IK)), poli(2-hidroksietilakrilat/itakonska kiselina) (P(HEA/IK)) i poli-(2-hidroksietilmetakrilat/poli(alkilenglikol)-(met)akrilati) (P(HEMA/BIS)). Komponenta koja se menja u sastavu hidrogela HEMA, HEA, kao i udeo komponente sa promenljivim sadržajem (udeo jonizujućeg komonomera (IK) i tip BIS komponente) definiše veličinu pora koja je dostupna za difuziju leka. U ovoj studiji su korišćeni lekovi bronhodilatori teofilin (TPH) i fenetilin-hidrohlorid (FE), i antibiotik cefaleksin (CEX). Ovi gelovi su klasifikovani u režimu poroznosti kao neporozni i mikroporozni, sa veličinom pora u opsegu 0,18-24,9 nm. Kontrolisano otpuštanje lekova je izvedeno u in vitro uslovima u puferu pH 7,40 i na 37 °C, da bi se odredili difuzioni koeficijenti leka u hidrogelovima. Na osnovu toga su predložene jednačine difuzije leka kroz hidrogel za svaki sistem lek/hidrogel. Rezultati dobijeni fitovanjem eksperimentalnih podataka su pokazali da difuzija leka zavisi od hemijske strukture i morfologije hidrogela i parametra λ, koji predstavlja odnos prečnika leka i veličine pora. Eksponencijalna zavisnost koeficijenta restrikcije od normalizovanog koeficijenta difuzije je dobijena za sisteme TPH/P(HEMA/IA), FE/P(HEMA/ /IA), CEX/P(HEMA/BIS) i CEX/P(HEA/IA).Utvrđeno je da veliki uticaj na difuziju leka imaju interakcije koje se odigravaju između funkcionalnih grupa leka i polimerne mreže.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Diffusion of drugs in hydrogels based on (meth)acrylates, poly(alkylene glycol) (meth)acrylates and itaconic acid
T1  - Difuzija lekova u hidrogelovima na bazi (met)akrilata, poli(alkilenglikol)-(met)akrilata i itakonske kiseline
EP  - 829
IS  - 6
SP  - 823
VL  - 66
DO  - 10.2298/HEMIND120406073B
ER  - 

@article{
author = "Babić, Marija and Jovašević, Jovana S. and Filipović, Jovanka M. and Tomić, Simonida",
year = "2012",
abstract = "The aim of this paper is to propose equations for the diffusion of drugs for investigated drug/hydrogel systems using the parameters affecting the transport of drug through poly- (2-hydroxyethylmethacrylate/itaconic acid) (P(HEMA/IA)), poly(2-hydroxyethylacrylate/ita- conic acid) (P(HEA/IA)), and poly(2-hydroxyethylmethacrylate/poly(alkyleneglycol) (meth)- acrylates) (P(HEMA/BIS)) copolymeric hydrogels. Different monomer types, as well as the variable content of some components in hydrogel composition (the amount of ionizable comonomer (IA) and different type of nonionic poly(alkyleneglycol) (meth)acrylates), ultimately defined the pore size available for drug diffusion. The hydrogels synthesized ranged from nonporous to microporous, based on the classification in accordance to the pore size, and could be classified as hydrogels that contain ionic groups and hydrogels without ionic groups. The drugs selected for this study are bronchodilators-theophylline (TPH), fenethylline hydrochloride (FE), and antibiotic cephalexin (CEX). Results of in vitro drug release tests defined the release systems based on the drug type, as well as the type of hydrogel used. The diffusion coefficient of drugs and the restriction coefficient, λ, defined as the ratio of solute to 'pore' radius (rs/rζ) that describes the ease of drug release from the gels, were used as factors that govern the release process., Cilj ove studije je da se predlože difuzione jednačine za ispitivane sisteme lek/hidrogel. Korišćeni su hidrogelovi poli(2-hidroksietilmetakrilat/itakonska kiselina) (P(HEMA/IK)), poli(2-hidroksietilakrilat/itakonska kiselina) (P(HEA/IK)) i poli-(2-hidroksietilmetakrilat/poli(alkilenglikol)-(met)akrilati) (P(HEMA/BIS)). Komponenta koja se menja u sastavu hidrogela HEMA, HEA, kao i udeo komponente sa promenljivim sadržajem (udeo jonizujućeg komonomera (IK) i tip BIS komponente) definiše veličinu pora koja je dostupna za difuziju leka. U ovoj studiji su korišćeni lekovi bronhodilatori teofilin (TPH) i fenetilin-hidrohlorid (FE), i antibiotik cefaleksin (CEX). Ovi gelovi su klasifikovani u režimu poroznosti kao neporozni i mikroporozni, sa veličinom pora u opsegu 0,18-24,9 nm. Kontrolisano otpuštanje lekova je izvedeno u in vitro uslovima u puferu pH 7,40 i na 37 °C, da bi se odredili difuzioni koeficijenti leka u hidrogelovima. Na osnovu toga su predložene jednačine difuzije leka kroz hidrogel za svaki sistem lek/hidrogel. Rezultati dobijeni fitovanjem eksperimentalnih podataka su pokazali da difuzija leka zavisi od hemijske strukture i morfologije hidrogela i parametra λ, koji predstavlja odnos prečnika leka i veličine pora. Eksponencijalna zavisnost koeficijenta restrikcije od normalizovanog koeficijenta difuzije je dobijena za sisteme TPH/P(HEMA/IA), FE/P(HEMA/ /IA), CEX/P(HEMA/BIS) i CEX/P(HEA/IA).Utvrđeno je da veliki uticaj na difuziju leka imaju interakcije koje se odigravaju između funkcionalnih grupa leka i polimerne mreže.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Diffusion of drugs in hydrogels based on (meth)acrylates, poly(alkylene glycol) (meth)acrylates and itaconic acid, Difuzija lekova u hidrogelovima na bazi (met)akrilata, poli(alkilenglikol)-(met)akrilata i itakonske kiseline",
pages = "829-823",
number = "6",
volume = "66",
doi = "10.2298/HEMIND120406073B"
}

Babić, M., Jovašević, J. S., Filipović, J. M.,& Tomić, S.. (2012). Diffusion of drugs in hydrogels based on (meth)acrylates, poly(alkylene glycol) (meth)acrylates and itaconic acid. in Hemijska industrija
Association of Chemical Engineers of Serbia., 66(6), 823-829.
https://doi.org/10.2298/HEMIND120406073B

Babić M, Jovašević JS, Filipović JM, Tomić S. Diffusion of drugs in hydrogels based on (meth)acrylates, poly(alkylene glycol) (meth)acrylates and itaconic acid. in Hemijska industrija. 2012;66(6):823-829.
doi:10.2298/HEMIND120406073B .

Babić, Marija, Jovašević, Jovana S., Filipović, Jovanka M., Tomić, Simonida, "Diffusion of drugs in hydrogels based on (meth)acrylates, poly(alkylene glycol) (meth)acrylates and itaconic acid" in Hemijska industrija, 66, no. 6 (2012):823-829,
https://doi.org/10.2298/HEMIND120406073B . .

TY  - CONF
AU  - Tomić, Simonida
AU  - Jovašević, Jovana S.
AU  - Perić-Grujić, Aleksandra
AU  - Dimitrijević, Suzana
AU  - Filipović, Jovanka M.
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2184
PB  - Springer, New York
C3  - 5th European Conference of the International Federation for Medical and Biological Engineering, Pts
T1  - Controlled Release and Antimicrobial Study of zinc(II)-Ion from zinc/poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hybrid Hydrogels
EP  - 974
SP  - 974
VL  - 37
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2184
ER  - 

@conference{
author = "Tomić, Simonida and Jovašević, Jovana S. and Perić-Grujić, Aleksandra and Dimitrijević, Suzana and Filipović, Jovanka M.",
year = "2012",
publisher = "Springer, New York",
journal = "5th European Conference of the International Federation for Medical and Biological Engineering, Pts",
title = "Controlled Release and Antimicrobial Study of zinc(II)-Ion from zinc/poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hybrid Hydrogels",
pages = "974-974",
volume = "37",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2184"
}

Tomić, S., Jovašević, J. S., Perić-Grujić, A., Dimitrijević, S.,& Filipović, J. M.. (2012). Controlled Release and Antimicrobial Study of zinc(II)-Ion from zinc/poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hybrid Hydrogels. in 5th European Conference of the International Federation for Medical and Biological Engineering, Pts
Springer, New York., 37, 974-974.
https://hdl.handle.net/21.15107/rcub_technorep_2184

Tomić S, Jovašević JS, Perić-Grujić A, Dimitrijević S, Filipović JM. Controlled Release and Antimicrobial Study of zinc(II)-Ion from zinc/poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hybrid Hydrogels. in 5th European Conference of the International Federation for Medical and Biological Engineering, Pts. 2012;37:974-974.
https://hdl.handle.net/21.15107/rcub_technorep_2184 .

Tomić, Simonida, Jovašević, Jovana S., Perić-Grujić, Aleksandra, Dimitrijević, Suzana, Filipović, Jovanka M., "Controlled Release and Antimicrobial Study of zinc(II)-Ion from zinc/poly(2-Hydroxyethyl Acrylate/Itaconic Acid) Hybrid Hydrogels" in 5th European Conference of the International Federation for Medical and Biological Engineering, Pts, 37 (2012):974-974,
https://hdl.handle.net/21.15107/rcub_technorep_2184 .

TY  - JOUR
AU  - Dobić, Sava N.
AU  - Filipović, Jovanka M.
AU  - Tomić, Simonida
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2213
AB  - Hydrogels based on 2-hydroxyethyl methacrylate (HEMA), different poly(ethylene glycol) dimethacrylates (PEGDMA) and itaconic acid (IA) were prepared by free radical crosslinking copolymerization. The influence of different PEGDMA content and type, with PEG chain length of 550 and 875, on the structure, morphology, biocompatibility, swelling and release properties of P(HEMA/IA/550PEGDMA) and P(HEMA/IA/875PEGDMA) copolymeric hydrogels have been investigated. Preliminary biocompatibility characterization of P(HEMA/IA/PEGDMA) hydrogels, done by the cytotoxicity assays using the HeLa cell line, indicated satisfactory cytocompatibility. Swelling studies were conducted for all samples in a physiological pH and temperature range, showing a noticeable pH sensitivity and temperature dependent equilibrium swelling. The characterization by Fourier transform infrared (FTIR) spectroscopy confirmed the presence of all monomers used in the gel structure, and morphology study, performed by scanning electron microscopy (SEM), revealed no significant differences between the samples with respect to pore size. In order to elucidate the release mechanism, drug release study was carried out in vitro using two forms of vitamin 6:3: nicotinamide (NAm) and nicotinic acid (NAc). The dissolution profiles were fitted to various mathematical models and the best fit was obtained for first order kinetics. The favorable swelling and drug release properties of P(HEMA/IA/PEGDMA) hydrogels make them good candidates to be used as biomaterials.
PB  - Elsevier Science Sa, Lausanne
T2  - Chemical Engineering Journal
T1  - Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid/poly(ethylene glycol) dimethacrylate) hydrogels
EP  - 380
SP  - 372
VL  - 179
DO  - 10.1016/j.cej.2011.10.083
ER  - 

@article{
author = "Dobić, Sava N. and Filipović, Jovanka M. and Tomić, Simonida",
year = "2012",
abstract = "Hydrogels based on 2-hydroxyethyl methacrylate (HEMA), different poly(ethylene glycol) dimethacrylates (PEGDMA) and itaconic acid (IA) were prepared by free radical crosslinking copolymerization. The influence of different PEGDMA content and type, with PEG chain length of 550 and 875, on the structure, morphology, biocompatibility, swelling and release properties of P(HEMA/IA/550PEGDMA) and P(HEMA/IA/875PEGDMA) copolymeric hydrogels have been investigated. Preliminary biocompatibility characterization of P(HEMA/IA/PEGDMA) hydrogels, done by the cytotoxicity assays using the HeLa cell line, indicated satisfactory cytocompatibility. Swelling studies were conducted for all samples in a physiological pH and temperature range, showing a noticeable pH sensitivity and temperature dependent equilibrium swelling. The characterization by Fourier transform infrared (FTIR) spectroscopy confirmed the presence of all monomers used in the gel structure, and morphology study, performed by scanning electron microscopy (SEM), revealed no significant differences between the samples with respect to pore size. In order to elucidate the release mechanism, drug release study was carried out in vitro using two forms of vitamin 6:3: nicotinamide (NAm) and nicotinic acid (NAc). The dissolution profiles were fitted to various mathematical models and the best fit was obtained for first order kinetics. The favorable swelling and drug release properties of P(HEMA/IA/PEGDMA) hydrogels make them good candidates to be used as biomaterials.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Chemical Engineering Journal",
title = "Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid/poly(ethylene glycol) dimethacrylate) hydrogels",
pages = "380-372",
volume = "179",
doi = "10.1016/j.cej.2011.10.083"
}

Dobić, S. N., Filipović, J. M.,& Tomić, S.. (2012). Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid/poly(ethylene glycol) dimethacrylate) hydrogels. in Chemical Engineering Journal
Elsevier Science Sa, Lausanne., 179, 372-380.
https://doi.org/10.1016/j.cej.2011.10.083

Dobić SN, Filipović JM, Tomić S. Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid/poly(ethylene glycol) dimethacrylate) hydrogels. in Chemical Engineering Journal. 2012;179:372-380.
doi:10.1016/j.cej.2011.10.083 .

Dobić, Sava N., Filipović, Jovanka M., Tomić, Simonida, "Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid/poly(ethylene glycol) dimethacrylate) hydrogels" in Chemical Engineering Journal, 179 (2012):372-380,
https://doi.org/10.1016/j.cej.2011.10.083 . .

TY  - JOUR
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Popović, Ivanka
AU  - Filipović, Jovanka M.
AU  - Kalagasidis Krušić, Melina
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1908
AB  - Novel pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid were prepared in two steps. Chitosan was first ionically crosslinked with itaconic acid, after which a free radical polymerization and crosslinking of the chitosan/itaconic acid network was performed by adding methacrylic acid and a crosslinker in order to achieve better mechanical properties and tunable swelling. The samples were characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, dynamic mechanical analysis and the swelling ratios of the hydrogels at various pH values (2.0-8.0). The hydrogel composition is found to have a great impact on the hydrogel structure, mechanical and thermal properties, morphology and swelling kinetics. The highly porous morphology of the gels is probably connected with the bulky chitosan/itaconic acid network which reduces the degree of crosslinking in the second step of the synthesis due to steric hindrances. The gels demonstrate substantial change in buffer absorbency with change of pH, low for acid buffers and the higher for pH values above 6 where the swelling is considerably slow, thus suggesting their strong candidature for use as oral drug-delivery systems in the lower parts of the gastrointestinal tract and for drugs that require longer release times.
PB  - Wiley-Blackwell, Hoboken
T2  - Polymer International
T1  - Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid
EP  - 452
IS  - 3
SP  - 443
VL  - 60
DO  - 10.1002/pi.2967
ER  - 

@article{
author = "Milosavljević, Nedeljko and Milašinović, Nikola and Popović, Ivanka and Filipović, Jovanka M. and Kalagasidis Krušić, Melina",
year = "2011",
abstract = "Novel pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid were prepared in two steps. Chitosan was first ionically crosslinked with itaconic acid, after which a free radical polymerization and crosslinking of the chitosan/itaconic acid network was performed by adding methacrylic acid and a crosslinker in order to achieve better mechanical properties and tunable swelling. The samples were characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, dynamic mechanical analysis and the swelling ratios of the hydrogels at various pH values (2.0-8.0). The hydrogel composition is found to have a great impact on the hydrogel structure, mechanical and thermal properties, morphology and swelling kinetics. The highly porous morphology of the gels is probably connected with the bulky chitosan/itaconic acid network which reduces the degree of crosslinking in the second step of the synthesis due to steric hindrances. The gels demonstrate substantial change in buffer absorbency with change of pH, low for acid buffers and the higher for pH values above 6 where the swelling is considerably slow, thus suggesting their strong candidature for use as oral drug-delivery systems in the lower parts of the gastrointestinal tract and for drugs that require longer release times.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Polymer International",
title = "Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid",
pages = "452-443",
number = "3",
volume = "60",
doi = "10.1002/pi.2967"
}

Milosavljević, N., Milašinović, N., Popović, I., Filipović, J. M.,& Kalagasidis Krušić, M.. (2011). Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid. in Polymer International
Wiley-Blackwell, Hoboken., 60(3), 443-452.
https://doi.org/10.1002/pi.2967

Milosavljević N, Milašinović N, Popović I, Filipović JM, Kalagasidis Krušić M. Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid. in Polymer International. 2011;60(3):443-452.
doi:10.1002/pi.2967 .

Milosavljević, Nedeljko, Milašinović, Nikola, Popović, Ivanka, Filipović, Jovanka M., Kalagasidis Krušić, Melina, "Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid" in Polymer International, 60, no. 3 (2011):443-452,
https://doi.org/10.1002/pi.2967 . .

TY  - JOUR
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Filipović, Jovanka M.
AU  - Kalagasidis Krušić, Melina
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1814
AB  - This paper deals with the synthesis of copolymer hydrogels of N-isopropylacrylamide, itaconic acid and chitosan. During synthesis, pH of the reaction solution and temperature were varied. It was found that the reaction at higher temperature, above lower critical solution temperature (LCST) value of N-isopropyl-acrylamide resulted in a formation of polymer rich domains or aggregates in the matrix of loosely tied network, which cause a higher degree of swelling. More-over, reaction pH also affects the degree of swelling and contraction of hydrogels owing to the different ionization of the ionic groups present in the hydrogel. The values of the diffusion exponent, n, indicate non-Fickian kinetics corresponding to diffusion and relaxation of polymer chains. These hydrogels could be classified as macroporous hydrogels. Hence, it is possible to synthesize hydrogels of desired functionality which allow their application in different fields. .
AB  - U ovom radu je prikazana sinteza kopolimernih hidrogelova N-izopropilakrilamida, itakonske kiseline i hitozana. Pri sintezi su varirani temperatura i pH vrednost rastvora za sintezu. Utvrđeno je da temperatura sinteze iznad donje kritične temperature rastvora (LCST) za NIPAM utiče na stvaranje agregata i elastičnih polimernih lanaca u mreži što omogućava veće bubrenje hidrogelova. Takođe je utvrđeno da i pH rastvora pri sintezi utiče na stepen bubrenja i kontrakcije hidrogelova, jer dolazi do različitog stepena jonizacije grupa koje ulaze u sastav hidrogela. Transport tečnosti kroz hidrogel ne prati Fick-ov zakon. Ovi hidrogelovi spadaju u grupu makroporoznih hidrogelova. Na ovaj način moguće je sintetisati hidrogelove željene funkcionalnosti što im omogućava primenu u raznim oblastima.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide
T1  - Sinteza i karakterizacija kopolimernih hidrogelova hitozana, itakonske kiseline i N-izopropilakrilamida
EP  - 666
IS  - 6
SP  - 657
VL  - 65
DO  - 10.2298/HEMIND110913072M
ER  - 

@article{
author = "Milosavljević, Nedeljko and Milašinović, Nikola and Filipović, Jovanka M. and Kalagasidis Krušić, Melina",
year = "2011",
abstract = "This paper deals with the synthesis of copolymer hydrogels of N-isopropylacrylamide, itaconic acid and chitosan. During synthesis, pH of the reaction solution and temperature were varied. It was found that the reaction at higher temperature, above lower critical solution temperature (LCST) value of N-isopropyl-acrylamide resulted in a formation of polymer rich domains or aggregates in the matrix of loosely tied network, which cause a higher degree of swelling. More-over, reaction pH also affects the degree of swelling and contraction of hydrogels owing to the different ionization of the ionic groups present in the hydrogel. The values of the diffusion exponent, n, indicate non-Fickian kinetics corresponding to diffusion and relaxation of polymer chains. These hydrogels could be classified as macroporous hydrogels. Hence, it is possible to synthesize hydrogels of desired functionality which allow their application in different fields. ., U ovom radu je prikazana sinteza kopolimernih hidrogelova N-izopropilakrilamida, itakonske kiseline i hitozana. Pri sintezi su varirani temperatura i pH vrednost rastvora za sintezu. Utvrđeno je da temperatura sinteze iznad donje kritične temperature rastvora (LCST) za NIPAM utiče na stvaranje agregata i elastičnih polimernih lanaca u mreži što omogućava veće bubrenje hidrogelova. Takođe je utvrđeno da i pH rastvora pri sintezi utiče na stepen bubrenja i kontrakcije hidrogelova, jer dolazi do različitog stepena jonizacije grupa koje ulaze u sastav hidrogela. Transport tečnosti kroz hidrogel ne prati Fick-ov zakon. Ovi hidrogelovi spadaju u grupu makroporoznih hidrogelova. Na ovaj način moguće je sintetisati hidrogelove željene funkcionalnosti što im omogućava primenu u raznim oblastima.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide, Sinteza i karakterizacija kopolimernih hidrogelova hitozana, itakonske kiseline i N-izopropilakrilamida",
pages = "666-657",
number = "6",
volume = "65",
doi = "10.2298/HEMIND110913072M"
}

Milosavljević, N., Milašinović, N., Filipović, J. M.,& Kalagasidis Krušić, M.. (2011). Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide. in Hemijska industrija
Association of Chemical Engineers of Serbia., 65(6), 657-666.
https://doi.org/10.2298/HEMIND110913072M

Milosavljević N, Milašinović N, Filipović JM, Kalagasidis Krušić M. Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide. in Hemijska industrija. 2011;65(6):657-666.
doi:10.2298/HEMIND110913072M .

Milosavljević, Nedeljko, Milašinović, Nikola, Filipović, Jovanka M., Kalagasidis Krušić, Melina, "Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide" in Hemijska industrija, 65, no. 6 (2011):657-666,
https://doi.org/10.2298/HEMIND110913072M . .