TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6441
AB  - The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.
PB  - Serbian Nutrition Society
C3  - 14th Congress of Nutrition
T1  - The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6441
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko",
year = "2021",
abstract = "The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.",
publisher = "Serbian Nutrition Society",
journal = "14th Congress of Nutrition",
title = "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6441"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K., Nedović, V.,& Bugarski, B.. (2021). The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition
Serbian Nutrition Society..
https://hdl.handle.net/21.15107/rcub_technorep_6441

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Nedović V, Bugarski B. The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition. 2021;:null-76.
https://hdl.handle.net/21.15107/rcub_technorep_6441 .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts" in 14th Congress of Nutrition (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6441 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6416
PB  - Serbian society for mitochondrial and free-radical physiology Ministry of Education
PB  - Science and Technological Development University of Belgrade
PB  - Faculty of Biology University of Belgrade
PB  - Institute for biological research “Siniša Stanković” University of Belgrade
PB  - Faculty of Pharmacy University of Belgrade
PB  - Faculty of Medicine
C3  - Third Congress REDOX MEDICINE Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease
T1  - Polyphenolic content and antioxidant activity of Thymus serpyllum extracts depending on extraction conditions
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6416
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2015",
publisher = "Serbian society for mitochondrial and free-radical physiology Ministry of Education, Science and Technological Development University of Belgrade, Faculty of Biology University of Belgrade, Institute for biological research “Siniša Stanković” University of Belgrade, Faculty of Pharmacy University of Belgrade, Faculty of Medicine",
journal = "Third Congress REDOX MEDICINE Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease",
title = "Polyphenolic content and antioxidant activity of Thymus serpyllum extracts depending on extraction conditions",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6416"
}

Jovanović, A. A., Petrović, P., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2015). Polyphenolic content and antioxidant activity of Thymus serpyllum extracts depending on extraction conditions. in Third Congress REDOX MEDICINE Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease
Serbian society for mitochondrial and free-radical physiology Ministry of Education..
https://hdl.handle.net/21.15107/rcub_technorep_6416

Jovanović AA, Petrović P, Zdunić G, Šavikin K, Bugarski B. Polyphenolic content and antioxidant activity of Thymus serpyllum extracts depending on extraction conditions. in Third Congress REDOX MEDICINE Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease. 2015;:null-26.
https://hdl.handle.net/21.15107/rcub_technorep_6416 .

Jovanović, Aleksandra A., Petrović, Predrag, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Polyphenolic content and antioxidant activity of Thymus serpyllum extracts depending on extraction conditions" in Third Congress REDOX MEDICINE Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease (2015),
https://hdl.handle.net/21.15107/rcub_technorep_6416 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina P.
AU  - Trifković, Kata
AU  - Jovanović, Jelena R.
AU  - Nedović, Viktor
AU  - Bugarski, Branko M.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6444
AB  - Aromatic plant species of family Lamiaceae are important medicinal plants because of their antioxidant, antimicrobial, antispasmodic, expectorant, stimulant and diuretic properties. In this study T. serpyllum herba was evaluated as a source of polyphenolic compounds, which are extracted by the application of ultrasound probe instead of the traditional solvent extraction. Ultrasound extraction has become a good alternative extraction method when compared to classical methods due to its high efficiency, low energy and solvent consumption. Ultrasound assisted extraction is a well established method in the processing of plant material, particularly to extract bioactive substances such as polyphenols. Optimization of the extraction was carried out through varying degrees of fragmentation (0.3, 0.7 and 1.5), time of extraction (3, 7 and 10 minutes) and ratio drugs:solvent (1:10, 1:20 and 1:30) in 30% ethanol as an extraction medium. Extraction efficiency was expressed via total polyphenols content (Folin-Ciocalteu method) and antioxidant activity (DPPH method). The highest total polyphenols content and antioxidant activity were recorded in extract with a degree of fragmentation of 0.3 and ratio drug:solvent 1:30 after 3 minutes, 23.03 mg/L GA and IC50 3.00 mg/ml, respectively. The lowest total polyphenols content and antioxidant capacity were obtained in extract with a degree of fragmentation of 1.5 and ratio drug:solvent 1:10 (12.06 mg/L GA and IC50 4.78 mg/ml, respectively). This study found that both total polyphenols and antioxidant activities determined were significantly affected by the degree of fragmentation and ratio drugs:solvent. Also, the levels of total soluble phenolic compounds were positively correlated with free radical scavenging activities against DPPH radicals.
PB  - School of Chemical Engineering, Athens
C3  - 29th EFFoST International Conference, Food Science Research and Innovation: Delivering sustainable solutions to the global economy and society
T1  - Ultrasound Extraction of Polyphenolic Compounds from Thymus serpyllum
EP  - 1368
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6444
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina P. and Trifković, Kata and Jovanović, Jelena R. and Nedović, Viktor and Bugarski, Branko M.",
year = "2015",
abstract = "Aromatic plant species of family Lamiaceae are important medicinal plants because of their antioxidant, antimicrobial, antispasmodic, expectorant, stimulant and diuretic properties. In this study T. serpyllum herba was evaluated as a source of polyphenolic compounds, which are extracted by the application of ultrasound probe instead of the traditional solvent extraction. Ultrasound extraction has become a good alternative extraction method when compared to classical methods due to its high efficiency, low energy and solvent consumption. Ultrasound assisted extraction is a well established method in the processing of plant material, particularly to extract bioactive substances such as polyphenols. Optimization of the extraction was carried out through varying degrees of fragmentation (0.3, 0.7 and 1.5), time of extraction (3, 7 and 10 minutes) and ratio drugs:solvent (1:10, 1:20 and 1:30) in 30% ethanol as an extraction medium. Extraction efficiency was expressed via total polyphenols content (Folin-Ciocalteu method) and antioxidant activity (DPPH method). The highest total polyphenols content and antioxidant activity were recorded in extract with a degree of fragmentation of 0.3 and ratio drug:solvent 1:30 after 3 minutes, 23.03 mg/L GA and IC50 3.00 mg/ml, respectively. The lowest total polyphenols content and antioxidant capacity were obtained in extract with a degree of fragmentation of 1.5 and ratio drug:solvent 1:10 (12.06 mg/L GA and IC50 4.78 mg/ml, respectively). This study found that both total polyphenols and antioxidant activities determined were significantly affected by the degree of fragmentation and ratio drugs:solvent. Also, the levels of total soluble phenolic compounds were positively correlated with free radical scavenging activities against DPPH radicals.",
publisher = "School of Chemical Engineering, Athens",
journal = "29th EFFoST International Conference, Food Science Research and Innovation: Delivering sustainable solutions to the global economy and society",
title = "Ultrasound Extraction of Polyphenolic Compounds from Thymus serpyllum",
pages = "1368",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6444"
}

Jovanović, A. A., Đorđević, V., Zdunić, G., Šavikin, K. P., Trifković, K., Jovanović, J. R., Nedović, V.,& Bugarski, B. M.. (2015). Ultrasound Extraction of Polyphenolic Compounds from Thymus serpyllum. in 29th EFFoST International Conference, Food Science Research and Innovation: Delivering sustainable solutions to the global economy and society
School of Chemical Engineering, Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6444

Jovanović AA, Đorđević V, Zdunić G, Šavikin KP, Trifković K, Jovanović JR, Nedović V, Bugarski BM. Ultrasound Extraction of Polyphenolic Compounds from Thymus serpyllum. in 29th EFFoST International Conference, Food Science Research and Innovation: Delivering sustainable solutions to the global economy and society. 2015;:null-1368.
https://hdl.handle.net/21.15107/rcub_technorep_6444 .

Jovanović, Aleksandra A., Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina P., Trifković, Kata, Jovanović, Jelena R., Nedović, Viktor, Bugarski, Branko M., "Ultrasound Extraction of Polyphenolic Compounds from Thymus serpyllum" in 29th EFFoST International Conference, Food Science Research and Innovation: Delivering sustainable solutions to the global economy and society (2015),
https://hdl.handle.net/21.15107/rcub_technorep_6444 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Pravilović, Radoslava
AU  - Đorđević, Verica
AU  - Isailović, Bojana
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6445
AB  - Wild Thyme (Thymus serpyllum) is perennial, herbaceous plant of Lamiaceae family. It has
aromatic, antioxidant, antiseptic, anthelmintic, repelent, antirheumatic, expectorant,
antispasmotic, carminative, sedative and diuretic properties. Optimization of the extraction by
maceration of T. serpyllum was carried out through varying degrees of fragmentation (0.3, 0.7
and 1.5) and the type of solvent (water, ethanol and mixtures of those in weight ratio 50:50 and
70:30). Extraction efficiency was expressed via total polyphenols content and antioxidant
activity. The highest total polyphenols content and antioxidant activity were recorded in 50%
ethanolic extract, with a degree of fragmentation of 0.3, 921,51 mg GA/g DW and 0,538 mM
Trolox/g DW, respectively. No significant differences were observed between total polyphenols
and antioxidant activity in water and in 70% ethanolic extracts. The lowest total polyphenols
content and antioxidant activity were obtained in 96% ethanolic extract (70.73 mg GA/g DW and
0,142 mM Trolox/g DW, respectively).
PB  - Faculty of Technology, Zvornik
C3  - IV International Congress of Engineering, Environment and Materials in Processing Industry
T1  - Effects of solvent and degree of fragmentation on total polyphenols and antioxidant activity of Thymus serpyllum extracts
EP  - 459
SP  - 453
DO  - 10.7251/EEMSR1501453
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Zdunić, Gordana and Šavikin, Katarina and Pravilović, Radoslava and Đorđević, Verica and Isailović, Bojana and Bugarski, Branko",
year = "2015",
abstract = "Wild Thyme (Thymus serpyllum) is perennial, herbaceous plant of Lamiaceae family. It has
aromatic, antioxidant, antiseptic, anthelmintic, repelent, antirheumatic, expectorant,
antispasmotic, carminative, sedative and diuretic properties. Optimization of the extraction by
maceration of T. serpyllum was carried out through varying degrees of fragmentation (0.3, 0.7
and 1.5) and the type of solvent (water, ethanol and mixtures of those in weight ratio 50:50 and
70:30). Extraction efficiency was expressed via total polyphenols content and antioxidant
activity. The highest total polyphenols content and antioxidant activity were recorded in 50%
ethanolic extract, with a degree of fragmentation of 0.3, 921,51 mg GA/g DW and 0,538 mM
Trolox/g DW, respectively. No significant differences were observed between total polyphenols
and antioxidant activity in water and in 70% ethanolic extracts. The lowest total polyphenols
content and antioxidant activity were obtained in 96% ethanolic extract (70.73 mg GA/g DW and
0,142 mM Trolox/g DW, respectively).",
publisher = "Faculty of Technology, Zvornik",
journal = "IV International Congress of Engineering, Environment and Materials in Processing Industry",
title = "Effects of solvent and degree of fragmentation on total polyphenols and antioxidant activity of Thymus serpyllum extracts",
pages = "459-453",
doi = "10.7251/EEMSR1501453"
}

Jovanović, A. A., Zdunić, G., Šavikin, K., Pravilović, R., Đorđević, V., Isailović, B.,& Bugarski, B.. (2015). Effects of solvent and degree of fragmentation on total polyphenols and antioxidant activity of Thymus serpyllum extracts. in IV International Congress of Engineering, Environment and Materials in Processing Industry
Faculty of Technology, Zvornik., 453-459.
https://doi.org/10.7251/EEMSR1501453

Jovanović AA, Zdunić G, Šavikin K, Pravilović R, Đorđević V, Isailović B, Bugarski B. Effects of solvent and degree of fragmentation on total polyphenols and antioxidant activity of Thymus serpyllum extracts. in IV International Congress of Engineering, Environment and Materials in Processing Industry. 2015;:453-459.
doi:10.7251/EEMSR1501453 .

Jovanović, Aleksandra A., Zdunić, Gordana, Šavikin, Katarina, Pravilović, Radoslava, Đorđević, Verica, Isailović, Bojana, Bugarski, Branko, "Effects of solvent and degree of fragmentation on total polyphenols and antioxidant activity of Thymus serpyllum extracts" in IV International Congress of Engineering, Environment and Materials in Processing Industry (2015):453-459,
https://doi.org/10.7251/EEMSR1501453 . .