TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6409
AB  - Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries.
AB  - Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.
PB  - Serbian Nutrition Society, Belgrade
T2  - Hrana i ishrana
T1  - Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques
EP  - 20
IS  - 2
SP  - 15
VL  - 62
DO  - 10.5937/hraIsh2102015J
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries., Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.",
publisher = "Serbian Nutrition Society, Belgrade",
journal = "Hrana i ishrana",
title = "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques",
pages = "20-15",
number = "2",
volume = "62",
doi = "10.5937/hraIsh2102015J"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana
Serbian Nutrition Society, Belgrade., 62(2), 15-20.
https://doi.org/10.5937/hraIsh2102015J

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Bugarski B. Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana. 2021;62(2):15-20.
doi:10.5937/hraIsh2102015J .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques" in Hrana i ishrana, 62, no. 2 (2021):15-20,
https://doi.org/10.5937/hraIsh2102015J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6410
AB  - Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Thymus serpyllum extract loaded liposomes produced by proliposome method
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6410
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Petrović, Predrag and Đorđević, Verica and Šavikin, Katarina and Zdunić, Gordana and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Thymus serpyllum extract loaded liposomes produced by proliposome method",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6410"
}

Jovanović, A. A., Balanč, B., Petrović, P., Đorđević, V., Šavikin, K., Zdunić, G.,& Bugarski, B.. (2021). Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference
University of Belgrade..
https://hdl.handle.net/21.15107/rcub_technorep_6410

Jovanović AA, Balanč B, Petrović P, Đorđević V, Šavikin K, Zdunić G, Bugarski B. Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference. 2021;:null-144.
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

Jovanović, Aleksandra A., Balanč, Bojana, Petrović, Predrag, Đorđević, Verica, Šavikin, Katarina, Zdunić, Gordana, Bugarski, Branko, "Thymus serpyllum extract loaded liposomes produced by proliposome method" in 2nd International UNIfood Conference (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Mijin, Dušan
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6443
AB  - Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.
PB  - Faculty of Technology, Zvornik
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
T1  - The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme
EP  - 143
SP  - 137
DO  - 10.7251/EEMEN2101137J
ER  - 

@conference{
author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Mijin, Dušan and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.",
publisher = "Faculty of Technology, Zvornik",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM",
title = "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme",
pages = "143-137",
doi = "10.7251/EEMEN2101137J"
}

Jovanović, A., Petrović, P., Đorđević, V., Mijin, D., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
Faculty of Technology, Zvornik., 137-143.
https://doi.org/10.7251/EEMEN2101137J

Jovanović A, Petrović P, Đorđević V, Mijin D, Zdunić G, Šavikin K, Bugarski B. The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM. 2021;:137-143.
doi:10.7251/EEMEN2101137J .

Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Mijin, Dušan, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme" in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM (2021):137-143,
https://doi.org/10.7251/EEMEN2101137J . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Skrt, Mihaela
AU  - Petrović, Predrag
AU  - Janković-Častvan, Ivona
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4288
AB  - The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity
EP  - 29
IS  - 39
SP  - 23
DO  - 10.5937/leksir1939023J
ER  - 

@article{
author = "Jovanović, Aleksandra and Skrt, Mihaela and Petrović, Predrag and Janković-Častvan, Ivona and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2019",
abstract = "The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity",
pages = "29-23",
number = "39",
doi = "10.5937/leksir1939023J"
}

Jovanović, A., Skrt, M., Petrović, P., Janković-Častvan, I., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2019). Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(39), 23-29.
https://doi.org/10.5937/leksir1939023J

Jovanović A, Skrt M, Petrović P, Janković-Častvan I, Zdunić G, Šavikin K, Bugarski B. Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine. 2019;(39):23-29.
doi:10.5937/leksir1939023J .

Jovanović, Aleksandra, Skrt, Mihaela, Petrović, Predrag, Janković-Častvan, Ivona, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity" in Lekovite sirovine, no. 39 (2019):23-29,
https://doi.org/10.5937/leksir1939023J . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3522
AB  - Different conventional (maceration and heat-assisted extraction) and new alternative techniques (ultrasound-assisted and microwave-assisted extractions) have been developed for the extraction of polyphenols compounds from different plant sources. Novel procedures have established with the aim to reduce the extraction time and solvent consumption, as well as to increase polyphenols yield and to improve extract quality. A critical review was conducted to introduce and compare traditional and modern procedures applied for extraction of bioactive polyphenols compounds. This review focuses on the different techniques of polyphenols extraction, discussing their operating conditions, mechanism, choice of particle size and solvent, solid/solvent ratio, extraction time, advantages/disadvantages and effectiveness. Finally, potential application of these extraction procedures in polyphenols isolation is reviewed.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Polyphenols extraction from plant sources
EP  - 49
IS  - 37
SP  - 45
DO  - 10.5937/leksir1737045J
ER  - 

@article{
author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2017",
abstract = "Different conventional (maceration and heat-assisted extraction) and new alternative techniques (ultrasound-assisted and microwave-assisted extractions) have been developed for the extraction of polyphenols compounds from different plant sources. Novel procedures have established with the aim to reduce the extraction time and solvent consumption, as well as to increase polyphenols yield and to improve extract quality. A critical review was conducted to introduce and compare traditional and modern procedures applied for extraction of bioactive polyphenols compounds. This review focuses on the different techniques of polyphenols extraction, discussing their operating conditions, mechanism, choice of particle size and solvent, solid/solvent ratio, extraction time, advantages/disadvantages and effectiveness. Finally, potential application of these extraction procedures in polyphenols isolation is reviewed.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Polyphenols extraction from plant sources",
pages = "49-45",
number = "37",
doi = "10.5937/leksir1737045J"
}

Jovanović, A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2017). Polyphenols extraction from plant sources. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(37), 45-49.
https://doi.org/10.5937/leksir1737045J

Jovanović A, Petrović P, Đorđević V, Zdunić G, Šavikin K, Bugarski B. Polyphenols extraction from plant sources. in Lekovite sirovine. 2017;(37):45-49.
doi:10.5937/leksir1737045J .

Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Polyphenols extraction from plant sources" in Lekovite sirovine, no. 37 (2017):45-49,
https://doi.org/10.5937/leksir1737045J . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Pljevljakusić, Dejan
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3751
AB  - Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE  gt  HAE  gt  maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.
PB  - Elsevier Science Bv, Amsterdam
T2  - Separation and Purification Technology
T1  - Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques
EP  - 380
SP  - 369
VL  - 179
DO  - 10.1016/j.seppur.2017.01.055
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Zdunić, Gordana and Pljevljakusić, Dejan and Šavikin, Katarina and Gođevac, Dejan and Bugarski, Branko",
year = "2017",
abstract = "Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE  gt  HAE  gt  maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Separation and Purification Technology",
title = "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques",
pages = "380-369",
volume = "179",
doi = "10.1016/j.seppur.2017.01.055"
}

Jovanović, A., Đorđević, V., Zdunić, G., Pljevljakusić, D., Šavikin, K., Gođevac, D.,& Bugarski, B.. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology
Elsevier Science Bv, Amsterdam., 179, 369-380.
https://doi.org/10.1016/j.seppur.2017.01.055

Jovanović A, Đorđević V, Zdunić G, Pljevljakusić D, Šavikin K, Gođevac D, Bugarski B. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology. 2017;179:369-380.
doi:10.1016/j.seppur.2017.01.055 .

Jovanović, Aleksandra, Đorđević, Verica, Zdunić, Gordana, Pljevljakusić, Dejan, Šavikin, Katarina, Gođevac, Dejan, Bugarski, Branko, "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques" in Separation and Purification Technology, 179 (2017):369-380,
https://doi.org/10.1016/j.seppur.2017.01.055 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Pljevljakusić, Dejan
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3291
AB  - The present study was designed to establish and optimize a method for extracting natural bioactive compounds from Thymus serpyllum which possess antioxidant, antimicrobial, antispasmodic and stimulant properties. Ultrasound-assisted extraction (UAE) is a welle-stablished method in the processing of plant material, particularly for extraction of bioactive substances such as polyphenols. The influential factors including extraction time (3, 7 and 10 min), solid:solvent ratio (1:10, 1:20 and 1:30) and particle size (0.3, 0.7 and 1.5 mm), have been studied to optimize the extraction process, while using 30% ethanol as an extraction medium and amplitude set to 65%. The yield of UAE was expressed via total phenol content and antioxidant activity of the obtained extracts. The optimum process parameters were found to be: extraction time, 3 min; solid:solvent ratio, 1:30; particle size, 0.3 mm. Under these conditions, the yield of total polyphenols was raised up to 23.03 mg/L GA and the highest antioxidant activity was recorded (10.32 mmol/mg Trolox and IC50 of 3.00 mg/ml).
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity
EP  - 398
IS  - 4
SP  - 391
VL  - 70
DO  - 10.2298/HEMIND150629044J
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Pljevljakusić, Dejan and Bugarski, Branko",
year = "2016",
abstract = "The present study was designed to establish and optimize a method for extracting natural bioactive compounds from Thymus serpyllum which possess antioxidant, antimicrobial, antispasmodic and stimulant properties. Ultrasound-assisted extraction (UAE) is a welle-stablished method in the processing of plant material, particularly for extraction of bioactive substances such as polyphenols. The influential factors including extraction time (3, 7 and 10 min), solid:solvent ratio (1:10, 1:20 and 1:30) and particle size (0.3, 0.7 and 1.5 mm), have been studied to optimize the extraction process, while using 30% ethanol as an extraction medium and amplitude set to 65%. The yield of UAE was expressed via total phenol content and antioxidant activity of the obtained extracts. The optimum process parameters were found to be: extraction time, 3 min; solid:solvent ratio, 1:30; particle size, 0.3 mm. Under these conditions, the yield of total polyphenols was raised up to 23.03 mg/L GA and the highest antioxidant activity was recorded (10.32 mmol/mg Trolox and IC50 of 3.00 mg/ml).",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity",
pages = "398-391",
number = "4",
volume = "70",
doi = "10.2298/HEMIND150629044J"
}

Jovanović, A., Đorđević, V., Zdunić, G., Šavikin, K., Pljevljakusić, D.,& Bugarski, B.. (2016). Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(4), 391-398.
https://doi.org/10.2298/HEMIND150629044J

Jovanović A, Đorđević V, Zdunić G, Šavikin K, Pljevljakusić D, Bugarski B. Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity. in Hemijska industrija. 2016;70(4):391-398.
doi:10.2298/HEMIND150629044J .

Jovanović, Aleksandra, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Pljevljakusić, Dejan, Bugarski, Branko, "Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity" in Hemijska industrija, 70, no. 4 (2016):391-398,
https://doi.org/10.2298/HEMIND150629044J . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Kalagasidis Krušić, Melina
AU  - Knežević-Jugović, Zorica
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3077
AB  - Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.
PB  - Sage Publications Ltd, London
T2  - Journal of Biomaterials Applications
T1  - Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties
EP  - 631
IS  - 5
SP  - 618
VL  - 30
DO  - 10.1177/0885328215598940
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica and Zdunić, Gordana and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko",
year = "2015",
abstract = "Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Biomaterials Applications",
title = "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties",
pages = "631-618",
number = "5",
volume = "30",
doi = "10.1177/0885328215598940"
}

Trifković, K. T., Milašinović, N., Đorđević, V., Zdunić, G., Kalagasidis Krušić, M., Knežević-Jugović, Z., Šavikin, K., Nedović, V.,& Bugarski, B.. (2015). Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of Biomaterials Applications
Sage Publications Ltd, London., 30(5), 618-631.
https://doi.org/10.1177/0885328215598940

Trifković KT, Milašinović N, Đorđević V, Zdunić G, Kalagasidis Krušić M, Knežević-Jugović Z, Šavikin K, Nedović V, Bugarski B. Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of Biomaterials Applications. 2015;30(5):618-631.
doi:10.1177/0885328215598940 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica, Zdunić, Gordana, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties" in Journal of Biomaterials Applications, 30, no. 5 (2015):618-631,
https://doi.org/10.1177/0885328215598940 . .

TY  - JOUR
AU  - Čančarević, Aleksandra
AU  - Bugarski, Branko
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2297
AB  - The Lamiaceae family, which contains about 200 genus and 3000 species, has a cosmopolitan distribution. Plants of this family are rich in essential oils and phenol compounds. Thymus vulgaris (Thyme) is perennial, subshrub plant which belongs to family of Labiates. Calyx and corolla are covered by dense gleaming glands that eradiate essential oil. Leaves or herba, and essential oils are used. Thymus serpyllum (Wild Thyme) is perennial, herbaceous plant. Leaves are evergreen, small and oval. Commitee on Herbal Medicinal Products (HMPC) classifies thyme herba and essential oil as a traditional herbal medicine used as expectorant in cough associated with cold. It is used in folk medicine to treat many diseases, including respiratory disease (cough pertussis, bronchitis and asthma), and has analgesic properties. Wild Thyme is approved for use in treatment of cough and bronchitis. It is unproven to treat kidney and bladder disorders. It is also used internally for dysmenorrheal, colic-like pain, and whooping cough. Thymol and carvacrol from essential oils of Thyme and Wild Thyme inhibit lipid peroxidation and manifest strong antimicrobial activity against different kinds of microorganisms. All the aromatic and medicinal plants available worldwide have been used for their preservative and medicinal values. Polyphenolic phytochemicals in traditionally used medicinal plants act as powerful antioxidants, which aroused an increasing interest in their application in functional food development. Recently, there has been growing considerable interest in extracts and essential oils of aromatic plants for the development of alternative food additives, in order to prevent the growth of food-borne pathogens or to delay the onset of food spoilage.
AB  - Familija Lamiaceae (Usnatice) obuhvata veliki broj kosmopolitskih vrsta, među kojima i vrste T. vulgaris (timijan) i T. serpyllum (majčina dušica), koje karakteriše prisustvo etarskih ulja i fenolnih jedinjenja. Timijan predstavlja višegodišnju polužbunastu biljku. Čašicu i krunicu gusto pokrivaju trihome u kojima se sintetišu i skladište etarska ulja. Koristi se list ili zeljasti nadzemni deo biljke u cvetu i etarsko ulje. Majčina dušica je višegodišnja zeljasta biljka. Cvetne grane su ravnomerno prekrivene žlezdanim trihomama. Koristi se nadzemni deo biljke u cvetu. Herbal Medicinal Product Committee (HMPC) klasifikuje herbu i etarsko ulje timijana kao tradicionalni biljni lek koji se koristi kao ekspektorans kod kašlja udruženog sa prehladom. Potvrđena primena majčine dušice je u tretmanu kašlja, bronhitisa i upalnih procesa gornjih disajnih puteva. Snažnu antioksidativnu aktivnost timijan duguje visokoj koncentraciji polifenolnih kiselina i flavonoidima. Timol i karvakrol prisutni u etarskom ulju majčine dušice inhibiraju lipidnu peroksidaciju i ispoljavaju antimikrobno dejstvo na različite vrste mikroorganizama. Dugogodišnja tradicionalna primena aromatičnih i lekovitih biljaka, pritisak potrošača da se toksični i kancerogeni sintetski aditivi zamene prirodnim supstancama i pojava multirezistentnih sojeva mikroorganizama povećavaju interes za istraživanjem datih biljnih vrsta, što bi omogućilo njihovu širu primenu u medicini, prehrambenoj, farmaceutskoj i kozmetičkoj industriji.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum
T1  - Biološka aktivnost vrsta Thymus vulgaris i Thymus serpyllum i njihovo korišćenje u etnomedicini
EP  - 17
IS  - 33
SP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2297
ER  - 

@article{
author = "Čančarević, Aleksandra and Bugarski, Branko and Šavikin, Katarina and Zdunić, Gordana",
year = "2013",
abstract = "The Lamiaceae family, which contains about 200 genus and 3000 species, has a cosmopolitan distribution. Plants of this family are rich in essential oils and phenol compounds. Thymus vulgaris (Thyme) is perennial, subshrub plant which belongs to family of Labiates. Calyx and corolla are covered by dense gleaming glands that eradiate essential oil. Leaves or herba, and essential oils are used. Thymus serpyllum (Wild Thyme) is perennial, herbaceous plant. Leaves are evergreen, small and oval. Commitee on Herbal Medicinal Products (HMPC) classifies thyme herba and essential oil as a traditional herbal medicine used as expectorant in cough associated with cold. It is used in folk medicine to treat many diseases, including respiratory disease (cough pertussis, bronchitis and asthma), and has analgesic properties. Wild Thyme is approved for use in treatment of cough and bronchitis. It is unproven to treat kidney and bladder disorders. It is also used internally for dysmenorrheal, colic-like pain, and whooping cough. Thymol and carvacrol from essential oils of Thyme and Wild Thyme inhibit lipid peroxidation and manifest strong antimicrobial activity against different kinds of microorganisms. All the aromatic and medicinal plants available worldwide have been used for their preservative and medicinal values. Polyphenolic phytochemicals in traditionally used medicinal plants act as powerful antioxidants, which aroused an increasing interest in their application in functional food development. Recently, there has been growing considerable interest in extracts and essential oils of aromatic plants for the development of alternative food additives, in order to prevent the growth of food-borne pathogens or to delay the onset of food spoilage., Familija Lamiaceae (Usnatice) obuhvata veliki broj kosmopolitskih vrsta, među kojima i vrste T. vulgaris (timijan) i T. serpyllum (majčina dušica), koje karakteriše prisustvo etarskih ulja i fenolnih jedinjenja. Timijan predstavlja višegodišnju polužbunastu biljku. Čašicu i krunicu gusto pokrivaju trihome u kojima se sintetišu i skladište etarska ulja. Koristi se list ili zeljasti nadzemni deo biljke u cvetu i etarsko ulje. Majčina dušica je višegodišnja zeljasta biljka. Cvetne grane su ravnomerno prekrivene žlezdanim trihomama. Koristi se nadzemni deo biljke u cvetu. Herbal Medicinal Product Committee (HMPC) klasifikuje herbu i etarsko ulje timijana kao tradicionalni biljni lek koji se koristi kao ekspektorans kod kašlja udruženog sa prehladom. Potvrđena primena majčine dušice je u tretmanu kašlja, bronhitisa i upalnih procesa gornjih disajnih puteva. Snažnu antioksidativnu aktivnost timijan duguje visokoj koncentraciji polifenolnih kiselina i flavonoidima. Timol i karvakrol prisutni u etarskom ulju majčine dušice inhibiraju lipidnu peroksidaciju i ispoljavaju antimikrobno dejstvo na različite vrste mikroorganizama. Dugogodišnja tradicionalna primena aromatičnih i lekovitih biljaka, pritisak potrošača da se toksični i kancerogeni sintetski aditivi zamene prirodnim supstancama i pojava multirezistentnih sojeva mikroorganizama povećavaju interes za istraživanjem datih biljnih vrsta, što bi omogućilo njihovu širu primenu u medicini, prehrambenoj, farmaceutskoj i kozmetičkoj industriji.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum, Biološka aktivnost vrsta Thymus vulgaris i Thymus serpyllum i njihovo korišćenje u etnomedicini",
pages = "17-3",
number = "33",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2297"
}

Čančarević, A., Bugarski, B., Šavikin, K.,& Zdunić, G.. (2013). Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(33), 3-17.
https://hdl.handle.net/21.15107/rcub_technorep_2297

Čančarević A, Bugarski B, Šavikin K, Zdunić G. Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum. in Lekovite sirovine. 2013;(33):3-17.
https://hdl.handle.net/21.15107/rcub_technorep_2297 .

Čančarević, Aleksandra, Bugarski, Branko, Šavikin, Katarina, Zdunić, Gordana, "Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum" in Lekovite sirovine, no. 33 (2013):3-17,
https://hdl.handle.net/21.15107/rcub_technorep_2297 .