TY  - JOUR
AU  - Moftah, Omar A.S.
AU  - Grbavčić, Sanja
AU  - Moftah, Walid A.S.
AU  - Luković, Nevena
AU  - Prodanović, Olivera
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2362
AB  - In this study, the solid and liquid wastes from the olive oil processing industry were evaluated as substrates for Yarrowia lipolytica growth with the aim of lipase production. Olive mill wastewater and olive oil cake seemed to provide the necessary nutrients and physical support for yeast growth and enzyme production. The highest lipolytic activity of 850 IU dm-3 was achieved after 4 days of submerged cultivation in supplemented olive mill wastewater. In addition, olive oil cake appeared to be a convenient substrate for lipase production under a solid-state fermentation mode. Lipase production was further improved by media supplementation and/or change in the physical settings of the experiment. However, the most significant improvement of lipase production under solid-state fermentation was achieved by an alkaline treatment of the substrate (more than 10-fold), when the amount of produced lipase reached up to ≈40 IU g-1 of substrate.
AB  - U ovom radu, tečne i čvrste otpadne sirovine koje zaostaju prilikom prerade maslina ispitane su kao potencijalni supstrati za rast kvasca Yarrowia lipolytica sa ciljem proizvodnje lipaza. Otpadna voda iz mlina, kao i pogača koja zaostaje nakon ceđenja ulja iz maslina, pokazali su se kao dobri izvori nutrijenata za rast ovog kvasca i proizvodnju enzima. U optimizovanoj tečnoj podlozi, prinos lipaza dostiže i do 850 IU dm-3. Pored toga, pogača koja zaostaje nakon ceđenja ulja iz maslina se pokazala kao pogodan čvrsti supstrat za gajenje proizvodnog mikroorganizma. Produkcija lipaza na ovom medijumu je dodatno optimizovana suplementacijom različitim izvorima azota i ugljenika, kao i promenom ostalih parametara fermentacije. Utvrđeno je da se najznačajnije poboljšanje produkcije lipaze ostvaruje alkalnim predtretmanom supstrata (više od 10 puta).
PB  - Serbian Chemical Society, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Lipase production by Yarrowia lipolytica using olive oil processing wastes as substrates
T1  - Proizvodnja lipaze iz Yarrowia lipolytica korišćenjem otpadnih sirovina industrije prerade maslina kao supstrata
EP  - 794
IS  - 6
SP  - 781
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2362
ER  - 

@article{
author = "Moftah, Omar A.S. and Grbavčić, Sanja and Moftah, Walid A.S. and Luković, Nevena and Prodanović, Olivera and Jakovetić, Sonja and Knežević-Jugović, Zorica",
year = "2013",
abstract = "In this study, the solid and liquid wastes from the olive oil processing industry were evaluated as substrates for Yarrowia lipolytica growth with the aim of lipase production. Olive mill wastewater and olive oil cake seemed to provide the necessary nutrients and physical support for yeast growth and enzyme production. The highest lipolytic activity of 850 IU dm-3 was achieved after 4 days of submerged cultivation in supplemented olive mill wastewater. In addition, olive oil cake appeared to be a convenient substrate for lipase production under a solid-state fermentation mode. Lipase production was further improved by media supplementation and/or change in the physical settings of the experiment. However, the most significant improvement of lipase production under solid-state fermentation was achieved by an alkaline treatment of the substrate (more than 10-fold), when the amount of produced lipase reached up to ≈40 IU g-1 of substrate., U ovom radu, tečne i čvrste otpadne sirovine koje zaostaju prilikom prerade maslina ispitane su kao potencijalni supstrati za rast kvasca Yarrowia lipolytica sa ciljem proizvodnje lipaza. Otpadna voda iz mlina, kao i pogača koja zaostaje nakon ceđenja ulja iz maslina, pokazali su se kao dobri izvori nutrijenata za rast ovog kvasca i proizvodnju enzima. U optimizovanoj tečnoj podlozi, prinos lipaza dostiže i do 850 IU dm-3. Pored toga, pogača koja zaostaje nakon ceđenja ulja iz maslina se pokazala kao pogodan čvrsti supstrat za gajenje proizvodnog mikroorganizma. Produkcija lipaza na ovom medijumu je dodatno optimizovana suplementacijom različitim izvorima azota i ugljenika, kao i promenom ostalih parametara fermentacije. Utvrđeno je da se najznačajnije poboljšanje produkcije lipaze ostvaruje alkalnim predtretmanom supstrata (više od 10 puta).",
publisher = "Serbian Chemical Society, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Lipase production by Yarrowia lipolytica using olive oil processing wastes as substrates, Proizvodnja lipaze iz Yarrowia lipolytica korišćenjem otpadnih sirovina industrije prerade maslina kao supstrata",
pages = "794-781",
number = "6",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2362"
}

Moftah, O. A.S., Grbavčić, S., Moftah, W. A.S., Luković, N., Prodanović, O., Jakovetić, S.,& Knežević-Jugović, Z.. (2013). Lipase production by Yarrowia lipolytica using olive oil processing wastes as substrates. in Journal of the Serbian Chemical Society
Serbian Chemical Society, Belgrade., 78(6), 781-794.
https://hdl.handle.net/21.15107/rcub_technorep_2362

Moftah OA, Grbavčić S, Moftah WA, Luković N, Prodanović O, Jakovetić S, Knežević-Jugović Z. Lipase production by Yarrowia lipolytica using olive oil processing wastes as substrates. in Journal of the Serbian Chemical Society. 2013;78(6):781-794.
https://hdl.handle.net/21.15107/rcub_technorep_2362 .

Moftah, Omar A.S., Grbavčić, Sanja, Moftah, Walid A.S., Luković, Nevena, Prodanović, Olivera, Jakovetić, Sonja, Knežević-Jugović, Zorica, "Lipase production by Yarrowia lipolytica using olive oil processing wastes as substrates" in Journal of the Serbian Chemical Society, 78, no. 6 (2013):781-794,
https://hdl.handle.net/21.15107/rcub_technorep_2362 .

TY  - JOUR
AU  - Moftah, Omar A.S.
AU  - Grbavčić, Sanja
AU  - Žuža, Milena
AU  - Luković, Nevena
AU  - Bezbradica, Dejan
AU  - Knežević-Jugović, Zorica
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2227
AB  - Olive oil cake is a by-product from the olive oil processing industry and can be used for the lipase and protease production by Candida utilis in solid state fermentation. Different carbon and nitrogen sources were evaluated, and the results showed that the supplementation of the substrate with maltose and starch as carbon sources and yeast extract as a nitrogen source significantly increased the lipase production. The best results were obtained with maltose, whereas rather low lipase and protease activities were found with glucose and oleic acid. Response surface methodology and a five-level-three-factor central composite rotatable design were used to evaluate the effects of the initial moisture content, inoculum size and fermentation time on both lipase and protease activity levels. A lipase activity value of approximate to 25 Ug(-1) and a protease activity value of 110 Ug(-1) were obtained under the optimized fermentation conditions. An alkaline treatment of the substrate appeared to be efficient, leading to increases of 39% and 133% in the lipase and protease production, respectively. The results showed that the olive cake could be a good source for enzyme production by solid state fermentation.
PB  - Springer, New York
T2  - Applied Biochemistry and Biotechnology
T1  - Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation
EP  - 364
IS  - 2
SP  - 348
VL  - 166
DO  - 10.1007/s12010-011-9429-2
ER  - 

@article{
author = "Moftah, Omar A.S. and Grbavčić, Sanja and Žuža, Milena and Luković, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica",
year = "2012",
abstract = "Olive oil cake is a by-product from the olive oil processing industry and can be used for the lipase and protease production by Candida utilis in solid state fermentation. Different carbon and nitrogen sources were evaluated, and the results showed that the supplementation of the substrate with maltose and starch as carbon sources and yeast extract as a nitrogen source significantly increased the lipase production. The best results were obtained with maltose, whereas rather low lipase and protease activities were found with glucose and oleic acid. Response surface methodology and a five-level-three-factor central composite rotatable design were used to evaluate the effects of the initial moisture content, inoculum size and fermentation time on both lipase and protease activity levels. A lipase activity value of approximate to 25 Ug(-1) and a protease activity value of 110 Ug(-1) were obtained under the optimized fermentation conditions. An alkaline treatment of the substrate appeared to be efficient, leading to increases of 39% and 133% in the lipase and protease production, respectively. The results showed that the olive cake could be a good source for enzyme production by solid state fermentation.",
publisher = "Springer, New York",
journal = "Applied Biochemistry and Biotechnology",
title = "Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation",
pages = "364-348",
number = "2",
volume = "166",
doi = "10.1007/s12010-011-9429-2"
}

Moftah, O. A.S., Grbavčić, S., Žuža, M., Luković, N., Bezbradica, D.,& Knežević-Jugović, Z.. (2012). Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation. in Applied Biochemistry and Biotechnology
Springer, New York., 166(2), 348-364.
https://doi.org/10.1007/s12010-011-9429-2

Moftah OA, Grbavčić S, Žuža M, Luković N, Bezbradica D, Knežević-Jugović Z. Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation. in Applied Biochemistry and Biotechnology. 2012;166(2):348-364.
doi:10.1007/s12010-011-9429-2 .

Moftah, Omar A.S., Grbavčić, Sanja, Žuža, Milena, Luković, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, "Adding Value to the Oil Cake as a Waste from Oil Processing Industry: Production of Lipase and Protease by Candida utilis in Solid State Fermentation" in Applied Biochemistry and Biotechnology, 166, no. 2 (2012):348-364,
https://doi.org/10.1007/s12010-011-9429-2 . .