TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Lađarević, Jelena
AU  - Onjia, Antonije
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7444
AB  - This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain
DO  - 10.1007/s11356-024-33367-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2024",
abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain",
doi = "10.1007/s11356-024-33367-x"
}

Ilić, N., Davidović, S., Milić, M., Lađarević, J., Onjia, A., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2024). Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research
Springer..
https://doi.org/10.1007/s11356-024-33367-x

Ilić N, Davidović S, Milić M, Lađarević J, Onjia A, Dimitrijević-Branković S, Mihajlovski K. Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research. 2024;.
doi:10.1007/s11356-024-33367-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Lađarević, Jelena, Onjia, Antonije, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain" in Environmental Science and Pollution Research (2024),
https://doi.org/10.1007/s11356-024-33367-x . .

TY  - CHAP
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Milić, Marija
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7427
AB  - Due to the depletion of fossil fuel sources and the issues posed by climate change, there is an increasing demand for renewable and sustainable fuel alternatives. Biofuels are a viable replacement for fossil fuels since they are made from renewable biomass. Degradation and valorization of renewable biomass start in the biorefinery with the main goal of producing biofuels and biochemicals in a more sustainable way. Lignocellulosic biomass (LB) presents one of the most abundant feedstocks for low-carbon fuel production. Enzymes belonging to cellulase, xylanase, pectinase, laccase, amylase, and lipase are utilized for biomass degradation and for the biofuels production. The emphasis of this chapter will be on the smart degrading enzymes designed to produce the two low-carbon biofuels, bioethanol and biodiesel, which are mostly used in the industrial sector. The technological development of commercial enzymes involved in biofuel and chemical production is also discussed.
PB  - Cham : Springer
T2  - Biorefinery and Industry 4.0: Empowering Sustainability
T1  - The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production
EP  - 179
SP  - 113
DO  - 10.1007/978-3-031-51601-6_5
ER  - 

@inbook{
author = "Mihajlovski, Katarina and Ilić, Nevena and Milić, Marija",
year = "2024",
abstract = "Due to the depletion of fossil fuel sources and the issues posed by climate change, there is an increasing demand for renewable and sustainable fuel alternatives. Biofuels are a viable replacement for fossil fuels since they are made from renewable biomass. Degradation and valorization of renewable biomass start in the biorefinery with the main goal of producing biofuels and biochemicals in a more sustainable way. Lignocellulosic biomass (LB) presents one of the most abundant feedstocks for low-carbon fuel production. Enzymes belonging to cellulase, xylanase, pectinase, laccase, amylase, and lipase are utilized for biomass degradation and for the biofuels production. The emphasis of this chapter will be on the smart degrading enzymes designed to produce the two low-carbon biofuels, bioethanol and biodiesel, which are mostly used in the industrial sector. The technological development of commercial enzymes involved in biofuel and chemical production is also discussed.",
publisher = "Cham : Springer",
journal = "Biorefinery and Industry 4.0: Empowering Sustainability",
booktitle = "The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production",
pages = "179-113",
doi = "10.1007/978-3-031-51601-6_5"
}

Mihajlovski, K., Ilić, N.,& Milić, M.. (2024). The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production. in Biorefinery and Industry 4.0: Empowering Sustainability
Cham : Springer., 113-179.
https://doi.org/10.1007/978-3-031-51601-6_5

Mihajlovski K, Ilić N, Milić M. The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production. in Biorefinery and Industry 4.0: Empowering Sustainability. 2024;:113-179.
doi:10.1007/978-3-031-51601-6_5 .

Mihajlovski, Katarina, Ilić, Nevena, Milić, Marija, "The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production" in Biorefinery and Industry 4.0: Empowering Sustainability (2024):113-179,
https://doi.org/10.1007/978-3-031-51601-6_5 . .

TY  - JOUR
AU  - Milić, Marija
AU  - Buntić, Aneta V.
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4981
AB  - This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.
T2  - Biomass Conversion and Biorefinery
T1  - The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization
EP  - 6759
IS  - 8
SP  - 6747
VL  - 13
DO  - 10.1007/s13399-021-01605-8
ER  - 

@article{
author = "Milić, Marija and Buntić, Aneta V. and Mihajlovski, Katarina and Ilić, Nevena and Davidović, Slađana and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.",
journal = "Biomass Conversion and Biorefinery",
title = "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization",
pages = "6759-6747",
number = "8",
volume = "13",
doi = "10.1007/s13399-021-01605-8"
}

Milić, M., Buntić, A. V., Mihajlovski, K., Ilić, N., Davidović, S.,& Dimitrijević-Branković, S.. (2023). The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery, 13(8), 6747-6759.
https://doi.org/10.1007/s13399-021-01605-8

Milić M, Buntić AV, Mihajlovski K, Ilić N, Davidović S, Dimitrijević-Branković S. The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery. 2023;13(8):6747-6759.
doi:10.1007/s13399-021-01605-8 .

Milić, Marija, Buntić, Aneta V., Mihajlovski, Katarina, Ilić, Nevena, Davidović, Slađana, Dimitrijević-Branković, Suzana, "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization" in Biomass Conversion and Biorefinery, 13, no. 8 (2023):6747-6759,
https://doi.org/10.1007/s13399-021-01605-8 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6605
AB  - Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
T1  - APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET
EP  - 186
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6605
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23",
title = "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET",
pages = "186-179",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6605"
}

Ilić, N., Milić, M., Davidović, S., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
Zvornik : University of East Sarajevo Faculty of Technology., 179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605

Ilić N, Milić M, Davidović S, Dimitrijević-Branković S, Mihajlovski K. APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23. 2023;:179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23 (2023):179-186,
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

TY  - JOUR
AU  - Papan Đaniš, Jelena
AU  - Periša, Jovana
AU  - Hribar Boštjančič, Patricija
AU  - Mihajlovski, Katarina
AU  - Lazić, Vesna
AU  - Dramićanin, Miroslav
AU  - Lisjak, Darja
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5864
AB  - Colloidal stabilization of magnetic nanoparticles is one of the most important steps in the preparation of magnetic nanoparticles for potential biomedical applications. A special kind of magnetic nanoparticle are barium hexaferrite nanoplatelets (BSHF NPLs) with a hexagonal shape and a permanent magnetic moment. One strategy for the stabilization of BHF in aqueous media is to use coatings. In our research, we used an eco-friendly tannic acid, as a coating on BSHF NPLs. As-prepared BSHF NPLs coated with tannic acid were examined with transmission electron microscopy, infrared and UV-Vis spectroscopy, electro-kinetic measurements, and their room-temperature magnetic properties were measured. Stable colloids were tested in two biological complex media and antimicrobial properties of the material were examined. To enhance the antimicrobial properties of our material, we used tannic acid as a platform for the in-situ production of silver on BSHF NPLs. New hybrid material with silver also possesses magnetic properties and excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus.
PB  - Elsevier B.V.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Barium hexaferrite nanoplatelets with polyphenol coatings for versatile applications as a stable, magnetic, and antimicrobial colloid
SP  - 113198
VL  - 224
DO  - 10.1016/j.colsurfb.2023.113198
ER  - 

@article{
author = "Papan Đaniš, Jelena and Periša, Jovana and Hribar Boštjančič, Patricija and Mihajlovski, Katarina and Lazić, Vesna and Dramićanin, Miroslav and Lisjak, Darja",
year = "2023",
abstract = "Colloidal stabilization of magnetic nanoparticles is one of the most important steps in the preparation of magnetic nanoparticles for potential biomedical applications. A special kind of magnetic nanoparticle are barium hexaferrite nanoplatelets (BSHF NPLs) with a hexagonal shape and a permanent magnetic moment. One strategy for the stabilization of BHF in aqueous media is to use coatings. In our research, we used an eco-friendly tannic acid, as a coating on BSHF NPLs. As-prepared BSHF NPLs coated with tannic acid were examined with transmission electron microscopy, infrared and UV-Vis spectroscopy, electro-kinetic measurements, and their room-temperature magnetic properties were measured. Stable colloids were tested in two biological complex media and antimicrobial properties of the material were examined. To enhance the antimicrobial properties of our material, we used tannic acid as a platform for the in-situ production of silver on BSHF NPLs. New hybrid material with silver also possesses magnetic properties and excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus.",
publisher = "Elsevier B.V.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Barium hexaferrite nanoplatelets with polyphenol coatings for versatile applications as a stable, magnetic, and antimicrobial colloid",
pages = "113198",
volume = "224",
doi = "10.1016/j.colsurfb.2023.113198"
}

Papan Đaniš, J., Periša, J., Hribar Boštjančič, P., Mihajlovski, K., Lazić, V., Dramićanin, M.,& Lisjak, D.. (2023). Barium hexaferrite nanoplatelets with polyphenol coatings for versatile applications as a stable, magnetic, and antimicrobial colloid. in Colloids and Surfaces B: Biointerfaces
Elsevier B.V.., 224, 113198.
https://doi.org/10.1016/j.colsurfb.2023.113198

Papan Đaniš J, Periša J, Hribar Boštjančič P, Mihajlovski K, Lazić V, Dramićanin M, Lisjak D. Barium hexaferrite nanoplatelets with polyphenol coatings for versatile applications as a stable, magnetic, and antimicrobial colloid. in Colloids and Surfaces B: Biointerfaces. 2023;224:113198.
doi:10.1016/j.colsurfb.2023.113198 .

Papan Đaniš, Jelena, Periša, Jovana, Hribar Boštjančič, Patricija, Mihajlovski, Katarina, Lazić, Vesna, Dramićanin, Miroslav, Lisjak, Darja, "Barium hexaferrite nanoplatelets with polyphenol coatings for versatile applications as a stable, magnetic, and antimicrobial colloid" in Colloids and Surfaces B: Biointerfaces, 224 (2023):113198,
https://doi.org/10.1016/j.colsurfb.2023.113198 . .

TY  - JOUR
AU  - Beluhan, Sunčica
AU  - Mihajlovski, Katarina
AU  - Šantek, Božidar
AU  - Ivančić Šantek, Mirela
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6728
AB  - Bioethanol is the most widely used alternative transportation fuel to petrol. Bioethanol is considered a clean, renewable, and environmentally friendly fuel that can contribute to climate change mitigation, decreased environmental pollution, and enhanced energy security. Commercial bioethanol production is based on traditional agricultural crops such as corn, sugarcane, and sugarbeet, primarily used as food and feed. In order to meet the growing demand for this fuel and decrease competition in the food and biofuel sectors for the same feedstock, other raw materials and process technologies have been intensively studied. Lignocellulosic biomass is one of the most abundant renewable resources, with it being rich in compounds that could be processed into energy, transportation fuels, various chemical compounds, and diverse materials. Bioethanol production from lignocellulosic biomass has received substantial attention in recent decades. This review gives an overview of bioethanol production steps from lignocellulosic biomass and challenges in the production process. The following aspects of bioethanol production are covered here, including pretreatment methods, process strategies, strain development, ethanol isolation and purification, and technical hurdles.
PB  - MDPI
T2  - Energies
T1  - The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing
IS  - 19
SP  - 7003
VL  - 16
DO  - 10.3390/en16197003
ER  - 

@article{
author = "Beluhan, Sunčica and Mihajlovski, Katarina and Šantek, Božidar and Ivančić Šantek, Mirela",
year = "2023",
abstract = "Bioethanol is the most widely used alternative transportation fuel to petrol. Bioethanol is considered a clean, renewable, and environmentally friendly fuel that can contribute to climate change mitigation, decreased environmental pollution, and enhanced energy security. Commercial bioethanol production is based on traditional agricultural crops such as corn, sugarcane, and sugarbeet, primarily used as food and feed. In order to meet the growing demand for this fuel and decrease competition in the food and biofuel sectors for the same feedstock, other raw materials and process technologies have been intensively studied. Lignocellulosic biomass is one of the most abundant renewable resources, with it being rich in compounds that could be processed into energy, transportation fuels, various chemical compounds, and diverse materials. Bioethanol production from lignocellulosic biomass has received substantial attention in recent decades. This review gives an overview of bioethanol production steps from lignocellulosic biomass and challenges in the production process. The following aspects of bioethanol production are covered here, including pretreatment methods, process strategies, strain development, ethanol isolation and purification, and technical hurdles.",
publisher = "MDPI",
journal = "Energies",
title = "The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing",
number = "19",
pages = "7003",
volume = "16",
doi = "10.3390/en16197003"
}

Beluhan, S., Mihajlovski, K., Šantek, B.,& Ivančić Šantek, M.. (2023). The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing. in Energies
MDPI., 16(19), 7003.
https://doi.org/10.3390/en16197003

Beluhan S, Mihajlovski K, Šantek B, Ivančić Šantek M. The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing. in Energies. 2023;16(19):7003.
doi:10.3390/en16197003 .

Beluhan, Sunčica, Mihajlovski, Katarina, Šantek, Božidar, Ivančić Šantek, Mirela, "The Production of Bioethanol from Lignocellulosic Biomass: Pretreatment Methods, Fermentation, and Downstream Processing" in Energies, 16, no. 19 (2023):7003,
https://doi.org/10.3390/en16197003 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6943
AB  - In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
T1  - IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES
EP  - 196
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6943
ER  - 

@conference{
author = "Ilić, Nevena and Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "In recent decades, enzyme immobilization on different supports occupies an important place in the modern biotechnology, given that it allows the design of green and sustainable production processes. Nanoparticles are very efficient supports for enzyme immobilization. The enzyme immobilized on nanoparticles is more stable than its soluble form and can be used in several operative cycles. In this study, the potential of modified titanium dioxide nanoparticles (Degussa P25, TiO2) for crude fungal laccase (47.43 U/g) immobilization was investigated. The TiO2 nanoparticles were modified with (3-glycidyloxypropyl) trimetoxylane under different conditions in order to obtain support with an optimal concentration of epoxy groups for immobilization. The obtained TiO2 nanoparticles had different concentrations of epoxy groups on their surface, exactly 950 μmol/g (TiO2M1) and 500 μmol/g (TiO2M2), respectively. The immobilization was carried out at pH 5 and at room temperature for 4 h. The residual activity of immobilized laccase on TiO2M2 was 33.40 %, while the residual activity of immobilized laccase on TiO2M1 was 17.39 %. The immobilization efficiency was 26.75 % and 20.18 % for laccase immobilized on TiO2M2 and immobilized laccase on TiO2M1, respectively. The immobilization of crude fungal laccase on TiO2M2 was further optimized by testing the influence of different contact time between laccase and TiO2M2 (2 h ,3 h, 3,5 h and 4 h) as well as the effect of various pH values (pH 4, pH 5 and pH 6) on immobilization. The highest residual activity of 35.04 % and immobilization efficiency of 28.95 % were obtained for optimal contact time of 3.5 h between crude fungal laccase and TiO2M2. The lowest residual activity (30.38 %) and immobilization efficiency (20.18 %) were obtained when immobilization was carried out at room temperature and at pH 5 for 2 h. The pH values had significant effect on immobilization. The optimal pH value was pH 5 with highest residual activity of 35.04 %, while the lowest residual activity of 14. 83 % was at pH 6. The residual activity of 21.21 % and immobilization efficency of 19.52 % were obtained when crude fungal laccase immobilized at pH 4. The immobilized laccase on TiO2M2 was successfully used in 5 cycles of guaiacol oxidation. In the 2nd cycle, the immobilized laccase had residual activity of about 60%, while after 3rd cycle, immobilized laccase had residual activity of 40 %. The lowest residual activity of 14.83 %, immobilized laccase had after 5th cycle.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023",
title = "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES",
pages = "196-187",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6943"
}

Ilić, N., Davidović, S., Miljković, M., Radovanović, N., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023
Zvornik : University of East Sarajevo Faculty of Technology., 187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943

Ilić N, Davidović S, Miljković M, Radovanović N, Dimitrijević-Branković S, Mihajlovski K. IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023. 2023;:187-196.
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

Ilić, Nevena, Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "IMMOBILIZATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. ON MODIFIED TITANIUM DIOXIDE NANOPARTICLES" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23, 2023 (2023):187-196,
https://hdl.handle.net/21.15107/rcub_technorep_6943 .

TY  - JOUR
AU  - Mihajlovski, Katarina R.
AU  - Stajčić, Željka
AU  - Lazić, Vesna M.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7038
AB  - The main goal of this work was to optimize the method of processing wound dressing products (like gauzes, sanitary pads, cotton wool, compresses, and bandages) with a commercial silver colloidal solution (Koloid doo, Belgrade, Serbia) and then to examine the antimicrobial properties of the obtained items in order to potentially reach the market with new improved wound dressing products. The influence of different silver concentrations used for treatment on antimicrobial activity was investigated only against Escherichia coli. The antimicrobial activity of different types of materials treated with silver solutions of 30 ppm was investigated against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis, and Enterococcus faecalis, as well as the fungus Candida albicans. The microbial reduction of the tested materials loaded with a silver solution of 30 ppm (15-20 µg of Ag on 1 g of fabric) against the Gram-negative bacteria E. coli and P. aeruginosa was almost maximal after 2 h of contact (i.e. 95 and 99 %, respectively). In the case of Gram-positive bacteria S. aureus, B. subtilis, and E. faecalis, a longer time is needed to completely eradicate bacteria (over 99 %). Antifungal activity testing against the fungus C. albicans gave moderate antifungal activity results.
AB  - Cilj ovog rada je ispitivanje antimikrobne efikasnosti pamučnih materijala kao što su gaza,
higijenski ulošci, vata, komprese i zavoji obrađeni komercijalnim koloidnim rastvorom srebra
(kompanija Koloid doo). Ispitan je uticaj koncentracije koloidnog rastvora srebra kojim je
obrađena tkanina na antimikrobnu aktivnost prema Gram-negativnim bakterijama Escherichia coli i Pseudomonas aeruginosa, Gram-pozitivnim bakterijama Staphylococcus aureus,
Bacillus subtilis i Enterococcus faecalis i gljivici Candida albicans. Redukcija bakterija postignuta testiranim materijalima obrađenim rastvorom srebra koncentracije 30 ppm (15 do
20 µg Ag na 1 g materijala) prema Gram-negativnim bakterijama E. coli i P. aeruginosa je
skoro maksimalna nakon dva sata kontakta, 95 i 99 %, redom. U slučaju Gram-pozitivnih
bakterija S. aureus, B. subtilis i E. faecalis, potrebno je duže vreme za potpunu redukciju broja
bakterija, osim za B. subtilis, gde je dovoljno dva sata kontakta za maksimalno smanjenje
početnog broja bakterija. Antifungalna aktivnost prema gljivici C. albicans je umerena.
PB  - Savez hemijskih inženjera Srbije
T2  - Hemijska industrija
T1  - Antimicrobial activity of different wound dressing products treated with silver
T1  - Antimikrobna aktivnost medicinskih materijala obrađenih srebrom
EP  - 273
IS  - 4
SP  - 265
VL  - 77
DO  - 10.2298/HEMIND230113021M
ER  - 

@article{
author = "Mihajlovski, Katarina R. and Stajčić, Željka and Lazić, Vesna M.",
year = "2023",
abstract = "The main goal of this work was to optimize the method of processing wound dressing products (like gauzes, sanitary pads, cotton wool, compresses, and bandages) with a commercial silver colloidal solution (Koloid doo, Belgrade, Serbia) and then to examine the antimicrobial properties of the obtained items in order to potentially reach the market with new improved wound dressing products. The influence of different silver concentrations used for treatment on antimicrobial activity was investigated only against Escherichia coli. The antimicrobial activity of different types of materials treated with silver solutions of 30 ppm was investigated against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis, and Enterococcus faecalis, as well as the fungus Candida albicans. The microbial reduction of the tested materials loaded with a silver solution of 30 ppm (15-20 µg of Ag on 1 g of fabric) against the Gram-negative bacteria E. coli and P. aeruginosa was almost maximal after 2 h of contact (i.e. 95 and 99 %, respectively). In the case of Gram-positive bacteria S. aureus, B. subtilis, and E. faecalis, a longer time is needed to completely eradicate bacteria (over 99 %). Antifungal activity testing against the fungus C. albicans gave moderate antifungal activity results., Cilj ovog rada je ispitivanje antimikrobne efikasnosti pamučnih materijala kao što su gaza,
higijenski ulošci, vata, komprese i zavoji obrađeni komercijalnim koloidnim rastvorom srebra
(kompanija Koloid doo). Ispitan je uticaj koncentracije koloidnog rastvora srebra kojim je
obrađena tkanina na antimikrobnu aktivnost prema Gram-negativnim bakterijama Escherichia coli i Pseudomonas aeruginosa, Gram-pozitivnim bakterijama Staphylococcus aureus,
Bacillus subtilis i Enterococcus faecalis i gljivici Candida albicans. Redukcija bakterija postignuta testiranim materijalima obrađenim rastvorom srebra koncentracije 30 ppm (15 do
20 µg Ag na 1 g materijala) prema Gram-negativnim bakterijama E. coli i P. aeruginosa je
skoro maksimalna nakon dva sata kontakta, 95 i 99 %, redom. U slučaju Gram-pozitivnih
bakterija S. aureus, B. subtilis i E. faecalis, potrebno je duže vreme za potpunu redukciju broja
bakterija, osim za B. subtilis, gde je dovoljno dva sata kontakta za maksimalno smanjenje
početnog broja bakterija. Antifungalna aktivnost prema gljivici C. albicans je umerena.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Antimicrobial activity of different wound dressing products treated with silver, Antimikrobna aktivnost medicinskih materijala obrađenih srebrom",
pages = "273-265",
number = "4",
volume = "77",
doi = "10.2298/HEMIND230113021M"
}

Mihajlovski, K. R., Stajčić, Ž.,& Lazić, V. M.. (2023). Antimicrobial activity of different wound dressing products treated with silver. in Hemijska industrija
Savez hemijskih inženjera Srbije., 77(4), 265-273.
https://doi.org/10.2298/HEMIND230113021M

Mihajlovski KR, Stajčić Ž, Lazić VM. Antimicrobial activity of different wound dressing products treated with silver. in Hemijska industrija. 2023;77(4):265-273.
doi:10.2298/HEMIND230113021M .

Mihajlovski, Katarina R., Stajčić, Željka, Lazić, Vesna M., "Antimicrobial activity of different wound dressing products treated with silver" in Hemijska industrija, 77, no. 4 (2023):265-273,
https://doi.org/10.2298/HEMIND230113021M . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Kostić, Anđela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5940
AB  - In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.
C3  - Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,
T1  - Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5940
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Kostić, Anđela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.",
journal = "Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,",
title = "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5940"
}

Ilić, N., Milić, M., Davidović, S., Kostić, A., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,, 80.
https://hdl.handle.net/21.15107/rcub_technorep_5940

Ilić N, Milić M, Davidović S, Kostić A, Mihajlovski K, Dimitrijević-Branković S. Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,. 2022;:80.
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Kostić, Anđela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix" in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering, (2022):80,
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

TY  - CHAP
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6145
AB  - The utilization of lignocellulosic biomass for biofuel production is a very important 
step in the transition towards to the circular economy. Within this process, the enzymatic 
hydrolysis are employed in a conversion of renewable biomass to a value-added product. 
The predominant polymer in lignocellulose is cellulose and enzymes involved in its 
degradation are cellulases. Those group of enzymes play a crucial role in biochemical degradation
of recalcitrant nature of plant lignocellulose, so marketable demand for them is rapidly growing. 
A leading enzyme manufacturers, like Novozymes, AB Enzymes, DSM, Genencor DuPont, have already developed a highly efficient cellulose-digesting enzymes cellulases for 
industrial use. Over time, those enzyme preparations have been improved and novel enzymes 
innovations in the form of enzyme cocktails have been launched on the market. Consecutively, 
this great expansion maintains a constant need among researchers for isolating and selecting 
the novel microorganisms, more potent producers of industrially powerful enzymes.
PB  - Wiley
T2  - Lignocellulose Bioconversion Through White Biotechnology
T1  - The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development
DO  - 10.1002/9781119735984.ch5
ER  - 

@inbook{
author = "Mihajlovski, Katarina and Milić, Marija",
year = "2022",
abstract = "The utilization of lignocellulosic biomass for biofuel production is a very important 
step in the transition towards to the circular economy. Within this process, the enzymatic 
hydrolysis are employed in a conversion of renewable biomass to a value-added product. 
The predominant polymer in lignocellulose is cellulose and enzymes involved in its 
degradation are cellulases. Those group of enzymes play a crucial role in biochemical degradation
of recalcitrant nature of plant lignocellulose, so marketable demand for them is rapidly growing. 
A leading enzyme manufacturers, like Novozymes, AB Enzymes, DSM, Genencor DuPont, have already developed a highly efficient cellulose-digesting enzymes cellulases for 
industrial use. Over time, those enzyme preparations have been improved and novel enzymes 
innovations in the form of enzyme cocktails have been launched on the market. Consecutively, 
this great expansion maintains a constant need among researchers for isolating and selecting 
the novel microorganisms, more potent producers of industrially powerful enzymes.",
publisher = "Wiley",
journal = "Lignocellulose Bioconversion Through White Biotechnology",
booktitle = "The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development",
doi = "10.1002/9781119735984.ch5"
}

Mihajlovski, K.,& Milić, M.. (2022). The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development. in Lignocellulose Bioconversion Through White Biotechnology
Wiley..
https://doi.org/10.1002/9781119735984.ch5

Mihajlovski K, Milić M. The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development. in Lignocellulose Bioconversion Through White Biotechnology. 2022;.
doi:10.1002/9781119735984.ch5 .

Mihajlovski, Katarina, Milić, Marija, "The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development" in Lignocellulose Bioconversion Through White Biotechnology (2022),
https://doi.org/10.1002/9781119735984.ch5 . .

TY  - CONF
AU  - Knežević, Magdalena
AU  - Stajković-Srbinović, Olivera
AU  - Maibassova, Assel
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Delić, Dušica
AU  - Buntić, Aneta
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6434
AB  - The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.
PB  - MDPI
C3  - 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
T1  - Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6434
ER  - 

@conference{
author = "Knežević, Magdalena and Stajković-Srbinović, Olivera and Maibassova, Assel and Milić, Marija and Mihajlovski, Katarina and Delić, Dušica and Buntić, Aneta",
year = "2022",
abstract = "The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.",
publisher = "MDPI",
journal = "1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection",
title = "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6434"
}

Knežević, M., Stajković-Srbinović, O., Maibassova, A., Milić, M., Mihajlovski, K., Delić, D.,& Buntić, A.. (2022). Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
MDPI..
https://hdl.handle.net/21.15107/rcub_technorep_6434

Knežević M, Stajković-Srbinović O, Maibassova A, Milić M, Mihajlovski K, Delić D, Buntić A. Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

Knežević, Magdalena, Stajković-Srbinović, Olivera, Maibassova, Assel, Milić, Marija, Mihajlovski, Katarina, Delić, Dušica, Buntić, Aneta, "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades" in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

TY  - CONF
AU  - Rogan, Jelena
AU  - Radovanović, Lidija
AU  - Simović, Bojana
AU  - Radovanović, Željko
AU  - Mihajlovski, Katarina
AU  - Dapčević, Aleksandra
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7404
AB  - Zinc oxide nanoparticles (ZnO-NPs) have been a subject of numerous researches owing to their multifunctional properties in many applications, such as solar cells, UV light-emitting devices, gas sensors and photocatalysts. ZnO-NPs can be synthesized through various methods and their features can be tailored by shape and size, resulting in new applications relevant to their structural properties. The synthesis of ZnO-NPs via controlled thermal decomposition of the single-source precursors represents a novel synthetic methodology. The basic goal of this study was to investigate the influence of precursors on crystallite size and morphology of the resulting ZnO-NPs. Four structurally characterized Zn complexes with different benzenepolycarboxylato ligands [1] were used as precursors for investigation of photocatalytic and antimicrobial effects of thermally obtained ZnO-NPs. The XRPD and FESEM analysis of ZnO-NPs, prepared by thermolysis of Zn precursors at 450 °C in the air atmosphere, revealed hexagonal wurtzite structure (space group P63mc, a ≈ 3.25 and c ≈ 5.21 Å) with an average crystallite size in the range of 39–47 nm and similar morphology. The best photocatalytic activity for degradation of Reactive orange 16 dye has been observed for ZnO-NPs where crystallites form the smallest agglomerates. All obtained ZnO-NPs showed excellent inhibitory effect against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli.
PB  - Zagreb : Croatian Association of Crystallographers
C3  - Book of Abstracts / The European Powder Diffraction Conference EPDIC 17, Šibenik, Croatia, 31 May – 03 June 2022
T1  - Photocatalytic and antimicrobial effects of zinc oxide nanoparticles prepared by thermal decomposition of zinc benzenepolycarboxylato complexes
SP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7404
ER  - 

@conference{
author = "Rogan, Jelena and Radovanović, Lidija and Simović, Bojana and Radovanović, Željko and Mihajlovski, Katarina and Dapčević, Aleksandra",
year = "2022",
abstract = "Zinc oxide nanoparticles (ZnO-NPs) have been a subject of numerous researches owing to their multifunctional properties in many applications, such as solar cells, UV light-emitting devices, gas sensors and photocatalysts. ZnO-NPs can be synthesized through various methods and their features can be tailored by shape and size, resulting in new applications relevant to their structural properties. The synthesis of ZnO-NPs via controlled thermal decomposition of the single-source precursors represents a novel synthetic methodology. The basic goal of this study was to investigate the influence of precursors on crystallite size and morphology of the resulting ZnO-NPs. Four structurally characterized Zn complexes with different benzenepolycarboxylato ligands [1] were used as precursors for investigation of photocatalytic and antimicrobial effects of thermally obtained ZnO-NPs. The XRPD and FESEM analysis of ZnO-NPs, prepared by thermolysis of Zn precursors at 450 °C in the air atmosphere, revealed hexagonal wurtzite structure (space group P63mc, a ≈ 3.25 and c ≈ 5.21 Å) with an average crystallite size in the range of 39–47 nm and similar morphology. The best photocatalytic activity for degradation of Reactive orange 16 dye has been observed for ZnO-NPs where crystallites form the smallest agglomerates. All obtained ZnO-NPs showed excellent inhibitory effect against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli.",
publisher = "Zagreb : Croatian Association of Crystallographers",
journal = "Book of Abstracts / The European Powder Diffraction Conference EPDIC 17, Šibenik, Croatia, 31 May – 03 June 2022",
title = "Photocatalytic and antimicrobial effects of zinc oxide nanoparticles prepared by thermal decomposition of zinc benzenepolycarboxylato complexes",
pages = "127",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7404"
}

Rogan, J., Radovanović, L., Simović, B., Radovanović, Ž., Mihajlovski, K.,& Dapčević, A.. (2022). Photocatalytic and antimicrobial effects of zinc oxide nanoparticles prepared by thermal decomposition of zinc benzenepolycarboxylato complexes. in Book of Abstracts / The European Powder Diffraction Conference EPDIC 17, Šibenik, Croatia, 31 May – 03 June 2022
Zagreb : Croatian Association of Crystallographers., 127.
https://hdl.handle.net/21.15107/rcub_technorep_7404

Rogan J, Radovanović L, Simović B, Radovanović Ž, Mihajlovski K, Dapčević A. Photocatalytic and antimicrobial effects of zinc oxide nanoparticles prepared by thermal decomposition of zinc benzenepolycarboxylato complexes. in Book of Abstracts / The European Powder Diffraction Conference EPDIC 17, Šibenik, Croatia, 31 May – 03 June 2022. 2022;:127.
https://hdl.handle.net/21.15107/rcub_technorep_7404 .

Rogan, Jelena, Radovanović, Lidija, Simović, Bojana, Radovanović, Željko, Mihajlovski, Katarina, Dapčević, Aleksandra, "Photocatalytic and antimicrobial effects of zinc oxide nanoparticles prepared by thermal decomposition of zinc benzenepolycarboxylato complexes" in Book of Abstracts / The European Powder Diffraction Conference EPDIC 17, Šibenik, Croatia, 31 May – 03 June 2022 (2022):127,
https://hdl.handle.net/21.15107/rcub_technorep_7404 .

TY  - JOUR
AU  - Grubišić, Marina
AU  - Perečinec Galić, Maja
AU  - Peremin, Ines
AU  - Mihajlovski, Katarina
AU  - Beluhan, Sunčica
AU  - Šantek, Božidar
AU  - Šantek Ivančić, Mirela
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5128
AB  - Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentration of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus.
PB  - MDPI
T2  - Energies
T1  - Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus
IS  - 9
SP  - 3208
VL  - 15
DO  - 10.3390/en15093208
ER  - 

@article{
author = "Grubišić, Marina and Perečinec Galić, Maja and Peremin, Ines and Mihajlovski, Katarina and Beluhan, Sunčica and Šantek, Božidar and Šantek Ivančić, Mirela",
year = "2022",
abstract = "Microbial lipids produced from lignocellulosic biomass are sustainable alternative feedstock for biodiesel production. In this study, corn cobs were used as a carbon source for lipid production and growth of oleaginous yeast Trichosporon oleaginosus. Lignocellulosic biomass was subjected to alkali and acid pretreatment using sulfuric acid and sodium hydroxide under different temperatures, catalyst concentrations and treatment times. Pretreatment of corn cobs was followed by cellulase hydrolysis. Hydrolysis of alkali pretreated (2% NaOH at 50◦C for 6 h, 1% NaOH at 50◦C for 16 h, 2% NaOH at 121◦C for 1 h, 1% NaOH at 121◦C for 2 h) and acid pretreated (1% H2SO4 120◦C for 20 min, and 2% H2SO4 120◦C for 10 min) corn cobs resulted in more than 80% of the theoretical yield of glucose. The effect of substrate (5, 10, 15 and 20%, g g−1 ) and cellulase loading (15 and 30 Filter Paper Units per gram of glucan, FPU g−1 ) on fermentable sugar yield was also studied. The maximal glucose concentration of 81.64 g L−1 was obtained from alkali-pretreated corn cobs (2% NaOH at 50◦C for 6 h) at 20% substrate loading and 30 FPU of Cellic CTec2 g−1 of glucan. Enzymatic hydrolysates of pretreated biomasses and filtrates of lignocellulosic slurries obtained after pretreatment were used for growth and lipid synthesis by T. oleaginosus. The highest lipid concentration of 18.97 g L−1 was obtained on hydrolysate of alkali-pretreated corn cobs (with 1% NaOH at 50◦C for 16 h) using a 15% (g g−1 ) substrate loading and 15 FPU g−1 of cellulase loading. Significant lipid accumulation was also achieved using undetoxified filtrates of pretreated slurries as substrates. Results showed that pretreated corn cobs and undetoxified filtrates are suitable carbon sources for the growth and efficient accumulation of lipids in T. oleaginosus.",
publisher = "MDPI",
journal = "Energies",
title = "Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus",
number = "9",
pages = "3208",
volume = "15",
doi = "10.3390/en15093208"
}

Grubišić, M., Perečinec Galić, M., Peremin, I., Mihajlovski, K., Beluhan, S., Šantek, B.,& Šantek Ivančić, M.. (2022). Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus. in Energies
MDPI., 15(9), 3208.
https://doi.org/10.3390/en15093208

Grubišić M, Perečinec Galić M, Peremin I, Mihajlovski K, Beluhan S, Šantek B, Šantek Ivančić M. Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus. in Energies. 2022;15(9):3208.
doi:10.3390/en15093208 .

Grubišić, Marina, Perečinec Galić, Maja, Peremin, Ines, Mihajlovski, Katarina, Beluhan, Sunčica, Šantek, Božidar, Šantek Ivančić, Mirela, "Optimization of Pretreatment Conditions and Enzymatic Hydrolysis of Corn Cobs for Production of Microbial Lipids by Trichosporon oleaginosus" in Energies, 15, no. 9 (2022):3208,
https://doi.org/10.3390/en15093208 . .

TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Gajić Savić, Ivana
AU  - Lađarević, Jelena
AU  - Milošević, Marija
AU  - Savić, Ivan
AU  - Mihajlovski, Katarina
AU  - Kostić, Mirjana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5237
AB  - A diluted ethanol orange peel extract was used for sustainable dyeing and functionalization of different fabrics. The extract analysis was performed using UPLC-ESI-MS/MS; its total flavonoid (0.67 g RE/100 g d.w.) and antioxidant (2.81 g GAE/100 g d.w.) contents and antioxidant activity (IC50 of 65.5 µg/mL) were also determined. The extract dyeing performance at various dyebath pH values was evaluated using multifiber fabric. Among six fabrics, extract possessed the ability for dyeing wool, polyamide, and cellulose acetate (at pH 4.5), which color strength (K/S) values increased after washing (9.7–19.8 vs. 11.6–23.2). Extract:water ratio of 20:35 (v/v) was found to be sufficient for achieving satisfactory K/S values (i.e., 20.17, 12.56, and 10.38 for wool, polyamide, and cellulose acetate, respectively) that were slightly changed after washing. The optimal dyeing temperatures for wool, polyamide, and cellulose acetate are 55, 35, and 25 °C, while the equilibrium dye exhaustion at those temperatures was achieved after 45, 120, and 90 min, respectively. The color coordinate measurements revealed that wool and polyamide fabrics are yellower than cellulose acetate, while, compared to polyamide and cellulose acetate, wool is redder. Possible interactions between selected fabrics and extract compounds are suggested. All fabrics possessed excellent antioxidant activity (88.6–99.6%) both before and after washing. Cellulose acetate provided maximum bacterial reduction (99.99%) for Escherichia coli, and Staphylococcus aureus, which in the case of Staphylococcus aureus remained unchanged after washing. Orange peel extract could be used for simultaneous dyeing and functionalization of wool and polyamide (excellent antioxidant activity) and cellulose acetate (excellent antioxidant and antibacterial activity) fabrics.
PB  - MDPI
T2  - Antioxidants
T1  - Sustainable Dyeing and Functionalization of Different Fibers Using Orange Peel Extract’s Antioxidants
IS  - 10
SP  - 2059
VL  - 11
DO  - 10.3390/antiox11102059
ER  - 

@article{
author = "Ivanovska, Aleksandra and Gajić Savić, Ivana and Lađarević, Jelena and Milošević, Marija and Savić, Ivan and Mihajlovski, Katarina and Kostić, Mirjana",
year = "2022",
abstract = "A diluted ethanol orange peel extract was used for sustainable dyeing and functionalization of different fabrics. The extract analysis was performed using UPLC-ESI-MS/MS; its total flavonoid (0.67 g RE/100 g d.w.) and antioxidant (2.81 g GAE/100 g d.w.) contents and antioxidant activity (IC50 of 65.5 µg/mL) were also determined. The extract dyeing performance at various dyebath pH values was evaluated using multifiber fabric. Among six fabrics, extract possessed the ability for dyeing wool, polyamide, and cellulose acetate (at pH 4.5), which color strength (K/S) values increased after washing (9.7–19.8 vs. 11.6–23.2). Extract:water ratio of 20:35 (v/v) was found to be sufficient for achieving satisfactory K/S values (i.e., 20.17, 12.56, and 10.38 for wool, polyamide, and cellulose acetate, respectively) that were slightly changed after washing. The optimal dyeing temperatures for wool, polyamide, and cellulose acetate are 55, 35, and 25 °C, while the equilibrium dye exhaustion at those temperatures was achieved after 45, 120, and 90 min, respectively. The color coordinate measurements revealed that wool and polyamide fabrics are yellower than cellulose acetate, while, compared to polyamide and cellulose acetate, wool is redder. Possible interactions between selected fabrics and extract compounds are suggested. All fabrics possessed excellent antioxidant activity (88.6–99.6%) both before and after washing. Cellulose acetate provided maximum bacterial reduction (99.99%) for Escherichia coli, and Staphylococcus aureus, which in the case of Staphylococcus aureus remained unchanged after washing. Orange peel extract could be used for simultaneous dyeing and functionalization of wool and polyamide (excellent antioxidant activity) and cellulose acetate (excellent antioxidant and antibacterial activity) fabrics.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Sustainable Dyeing and Functionalization of Different Fibers Using Orange Peel Extract’s Antioxidants",
number = "10",
pages = "2059",
volume = "11",
doi = "10.3390/antiox11102059"
}

Ivanovska, A., Gajić Savić, I., Lađarević, J., Milošević, M., Savić, I., Mihajlovski, K.,& Kostić, M.. (2022). Sustainable Dyeing and Functionalization of Different Fibers Using Orange Peel Extract’s Antioxidants. in Antioxidants
MDPI., 11(10), 2059.
https://doi.org/10.3390/antiox11102059

Ivanovska A, Gajić Savić I, Lađarević J, Milošević M, Savić I, Mihajlovski K, Kostić M. Sustainable Dyeing and Functionalization of Different Fibers Using Orange Peel Extract’s Antioxidants. in Antioxidants. 2022;11(10):2059.
doi:10.3390/antiox11102059 .

Ivanovska, Aleksandra, Gajić Savić, Ivana, Lađarević, Jelena, Milošević, Marija, Savić, Ivan, Mihajlovski, Katarina, Kostić, Mirjana, "Sustainable Dyeing and Functionalization of Different Fibers Using Orange Peel Extract’s Antioxidants" in Antioxidants, 11, no. 10 (2022):2059,
https://doi.org/10.3390/antiox11102059 . .

TY  - CONF
AU  - Vasić, Katja
AU  - Ilić, Nevena
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
AU  - Knez, Željko
AU  - Leitgeb, Maja
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5941
AB  - The principles of green chemistry encourage simple and eco-friendly approaches,
using various enzymes for different industries, such as fine chemicals productions
and water treatments. To increase operational stability of enzymes, different immobilization techniques must be employed in preparation of highly active
biocatalysts. Entrapment of enzymes within a polymer matrix is the simplest immobilization method. Properties, such as size
of beads, porosity, enzyme leakage degree,
and stability are optimized through alginate and calcium concentrations and the
use of cross-linking agents.
Alginate beads were prepared in calcium chloride and later on activated using
cross-linking agent glutaraldehyde (GA),
where GA concentration and time of activation were optimized. Such GA-activated
alginate beads were further immobilized
with laccase, where the time of immobilization was optimized. Immobilization efficiency and residual activity were determined after optimization protocol,
resulting in 97 % and 88 %, respectively.
Results indicate that laccase can be immobilized onto alginate beads and their residual activity can be improved by varying
the GA concentration for beads’ activation. Such GA-activated alginate beads can
be successfully used for laccase immobilization for further applications.
PB  - Wiley
C3  - Book of Abstracts- Chemie Ingenieur Technik
T1  - Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde
IS  - 9
SP  - 1266
VL  - 94
DO  - 10.1002/cite.202255288
ER  - 

@conference{
author = "Vasić, Katja and Ilić, Nevena and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana and Knez, Željko and Leitgeb, Maja",
year = "2022",
abstract = "The principles of green chemistry encourage simple and eco-friendly approaches,
using various enzymes for different industries, such as fine chemicals productions
and water treatments. To increase operational stability of enzymes, different immobilization techniques must be employed in preparation of highly active
biocatalysts. Entrapment of enzymes within a polymer matrix is the simplest immobilization method. Properties, such as size
of beads, porosity, enzyme leakage degree,
and stability are optimized through alginate and calcium concentrations and the
use of cross-linking agents.
Alginate beads were prepared in calcium chloride and later on activated using
cross-linking agent glutaraldehyde (GA),
where GA concentration and time of activation were optimized. Such GA-activated
alginate beads were further immobilized
with laccase, where the time of immobilization was optimized. Immobilization efficiency and residual activity were determined after optimization protocol,
resulting in 97 % and 88 %, respectively.
Results indicate that laccase can be immobilized onto alginate beads and their residual activity can be improved by varying
the GA concentration for beads’ activation. Such GA-activated alginate beads can
be successfully used for laccase immobilization for further applications.",
publisher = "Wiley",
journal = "Book of Abstracts- Chemie Ingenieur Technik",
title = "Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde",
number = "9",
pages = "1266",
volume = "94",
doi = "10.1002/cite.202255288"
}

Vasić, K., Ilić, N., Mihajlovski, K., Dimitrijević-Branković, S., Knez, Ž.,& Leitgeb, M.. (2022). Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde. in Book of Abstracts- Chemie Ingenieur Technik
Wiley., 94(9), 1266.
https://doi.org/10.1002/cite.202255288

Vasić K, Ilić N, Mihajlovski K, Dimitrijević-Branković S, Knez Ž, Leitgeb M. Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde. in Book of Abstracts- Chemie Ingenieur Technik. 2022;94(9):1266.
doi:10.1002/cite.202255288 .

Vasić, Katja, Ilić, Nevena, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, Knez, Željko, Leitgeb, Maja, "Immobilization of laccase onto sodium alginate beads activated with glutaraldehyde" in Book of Abstracts- Chemie Ingenieur Technik, 94, no. 9 (2022):1266,
https://doi.org/10.1002/cite.202255288 . .

TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Lađarević, Jelena
AU  - Asanović, Koviljka
AU  - Barać, Nemanja
AU  - Mihajlovski, Katarina
AU  - Kostić, Mirjana
AU  - Mangovska, Biljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5968
AB  - The quality of cotton and cotton/elastane single jersey knitted fabrics before and after softening was evaluated through their mechanical properties, pilling, and volume electrical resistivity. Elastane-containing fabrics have higher bursting elongation and stiffness, lower bursting strength, the same or higher pilling grade, and 23–27% lower electrical resistivities compared to 100% cotton due to the increased compactness that enables better contact between the loops, and easier flow of charge through them. Softening does not affect or slightly improves the fabric pilling (up to 1000 cycles); it decreases fabrics’ stiffness and increases their bursting strength and electrical resistivities. The last one was significantly decreased after the in situ synthesis of Cu-based nanoparticles on fabrics’ surfaces. Namely, fabrics were first treated with sodium alginate whose carboxylate groups crosslinked Cu2+-ions (from CuSO4 solution) that were further reduced with ascorbic acid. In situ synthesis of Cu-based nanoparticles (detected by FESEM) improves the fabrics’ quality and enables obtaining multifunctional fabrics having 3.4–9.6 times lower resistivities, which are in line with the content of Cu after reduction (determined using ICP-OES), up to 2.5 times higher antioxidant activity and provided maximum microbial reduction for E. Coli, S. aureus, and C. albicans.
PB  - Taylor and Francis Ltd.
T2  - Journal of Natural Fibers
T1  - Quality of Cotton and cotton/elastane Single Jersey Knitted Fabrics before and after Softening and in Situ Synthesis of Cu-based Nanoparticles
EP  - 15150
IS  - 16
SP  - 15139
VL  - 19
DO  - 10.1080/15440478.2022.2070328
ER  - 

@article{
author = "Ivanovska, Aleksandra and Lađarević, Jelena and Asanović, Koviljka and Barać, Nemanja and Mihajlovski, Katarina and Kostić, Mirjana and Mangovska, Biljana",
year = "2022",
abstract = "The quality of cotton and cotton/elastane single jersey knitted fabrics before and after softening was evaluated through their mechanical properties, pilling, and volume electrical resistivity. Elastane-containing fabrics have higher bursting elongation and stiffness, lower bursting strength, the same or higher pilling grade, and 23–27% lower electrical resistivities compared to 100% cotton due to the increased compactness that enables better contact between the loops, and easier flow of charge through them. Softening does not affect or slightly improves the fabric pilling (up to 1000 cycles); it decreases fabrics’ stiffness and increases their bursting strength and electrical resistivities. The last one was significantly decreased after the in situ synthesis of Cu-based nanoparticles on fabrics’ surfaces. Namely, fabrics were first treated with sodium alginate whose carboxylate groups crosslinked Cu2+-ions (from CuSO4 solution) that were further reduced with ascorbic acid. In situ synthesis of Cu-based nanoparticles (detected by FESEM) improves the fabrics’ quality and enables obtaining multifunctional fabrics having 3.4–9.6 times lower resistivities, which are in line with the content of Cu after reduction (determined using ICP-OES), up to 2.5 times higher antioxidant activity and provided maximum microbial reduction for E. Coli, S. aureus, and C. albicans.",
publisher = "Taylor and Francis Ltd.",
journal = "Journal of Natural Fibers",
title = "Quality of Cotton and cotton/elastane Single Jersey Knitted Fabrics before and after Softening and in Situ Synthesis of Cu-based Nanoparticles",
pages = "15150-15139",
number = "16",
volume = "19",
doi = "10.1080/15440478.2022.2070328"
}

Ivanovska, A., Lađarević, J., Asanović, K., Barać, N., Mihajlovski, K., Kostić, M.,& Mangovska, B.. (2022). Quality of Cotton and cotton/elastane Single Jersey Knitted Fabrics before and after Softening and in Situ Synthesis of Cu-based Nanoparticles. in Journal of Natural Fibers
Taylor and Francis Ltd.., 19(16), 15139-15150.
https://doi.org/10.1080/15440478.2022.2070328

Ivanovska A, Lađarević J, Asanović K, Barać N, Mihajlovski K, Kostić M, Mangovska B. Quality of Cotton and cotton/elastane Single Jersey Knitted Fabrics before and after Softening and in Situ Synthesis of Cu-based Nanoparticles. in Journal of Natural Fibers. 2022;19(16):15139-15150.
doi:10.1080/15440478.2022.2070328 .

Ivanovska, Aleksandra, Lađarević, Jelena, Asanović, Koviljka, Barać, Nemanja, Mihajlovski, Katarina, Kostić, Mirjana, Mangovska, Biljana, "Quality of Cotton and cotton/elastane Single Jersey Knitted Fabrics before and after Softening and in Situ Synthesis of Cu-based Nanoparticles" in Journal of Natural Fibers, 19, no. 16 (2022):15139-15150,
https://doi.org/10.1080/15440478.2022.2070328 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/20
AB  - Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Waste and Biomass Valorization
T1  - From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production
EP  - 174
IS  - 1
SP  - 165
VL  - 12
DO  - 10.1007/s12649-020-00960-3
ER  - 

@article{
author = "Mihajlovski, Katarina and Buntić, Aneta and Milić, Marija and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Waste and Biomass Valorization",
title = "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production",
pages = "174-165",
number = "1",
volume = "12",
doi = "10.1007/s12649-020-00960-3"
}

Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization
Springer, Dordrecht., 12(1), 165-174.
https://doi.org/10.1007/s12649-020-00960-3

Mihajlovski K, Buntić A, Milić M, Rajilić-Stojanović M, Dimitrijević-Branković S. From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization. 2021;12(1):165-174.
doi:10.1007/s12649-020-00960-3 .

Mihajlovski, Katarina, Buntić, Aneta, Milić, Marija, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production" in Waste and Biomass Valorization, 12, no. 1 (2021):165-174,
https://doi.org/10.1007/s12649-020-00960-3 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
AU  - Pecarski, Danijela
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4853
AB  - A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.
T2  - Journal of the Serbian Chemical Society
T1  - Statistical optimization of bioethanol production from waste bread hydrolysate
EP  - 662
IS  - 7-8
SP  - 651
VL  - 86
DO  - 10.2298/JSC210308032M
ER  - 

@article{
author = "Mihajlovski, Katarina and Milić, Marija and Pecarski, Danijela and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.",
journal = "Journal of the Serbian Chemical Society",
title = "Statistical optimization of bioethanol production from waste bread hydrolysate",
pages = "662-651",
number = "7-8",
volume = "86",
doi = "10.2298/JSC210308032M"
}

Mihajlovski, K., Milić, M., Pecarski, D.,& Dimitrijević-Branković, S.. (2021). Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society, 86(7-8), 651-662.
https://doi.org/10.2298/JSC210308032M

Mihajlovski K, Milić M, Pecarski D, Dimitrijević-Branković S. Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society. 2021;86(7-8):651-662.
doi:10.2298/JSC210308032M .

Mihajlovski, Katarina, Milić, Marija, Pecarski, Danijela, Dimitrijević-Branković, Suzana, "Statistical optimization of bioethanol production from waste bread hydrolysate" in Journal of the Serbian Chemical Society, 86, no. 7-8 (2021):651-662,
https://doi.org/10.2298/JSC210308032M . .

TY  - JOUR
AU  - Knezevic, Magdalena M.
AU  - Stajkovic-Srbinovic, Olivera S.
AU  - Assel, Maibassova
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Delic, Dusica, I
AU  - Buntić, Aneta V.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4919
AB  - This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.
T2  - Rhizosphere
T1  - The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium
VL  - 18
DO  - 10.1016/j.rhisph.2021.100353
ER  - 

@article{
author = "Knezevic, Magdalena M. and Stajkovic-Srbinovic, Olivera S. and Assel, Maibassova and Milić, Marija and Mihajlovski, Katarina and Delic, Dusica, I and Buntić, Aneta V.",
year = "2021",
abstract = "This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.",
journal = "Rhizosphere",
title = "The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium",
volume = "18",
doi = "10.1016/j.rhisph.2021.100353"
}

Knezevic, M. M., Stajkovic-Srbinovic, O. S., Assel, M., Milić, M., Mihajlovski, K., Delic, D. I.,& Buntić, A. V.. (2021). The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium. in Rhizosphere, 18.
https://doi.org/10.1016/j.rhisph.2021.100353

Knezevic MM, Stajkovic-Srbinovic OS, Assel M, Milić M, Mihajlovski K, Delic DI, Buntić AV. The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium. in Rhizosphere. 2021;18.
doi:10.1016/j.rhisph.2021.100353 .

Knezevic, Magdalena M., Stajkovic-Srbinovic, Olivera S., Assel, Maibassova, Milić, Marija, Mihajlovski, Katarina, Delic, Dusica, I, Buntić, Aneta V., "The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium" in Rhizosphere, 18 (2021),
https://doi.org/10.1016/j.rhisph.2021.100353 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5939
AB  - The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.
C3  - Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021
T1  - The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5939
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.",
journal = "Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021",
title = "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5939"
}

Ilić, N., Milić, M., Davidović, S., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2021). The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021, 158.
https://hdl.handle.net/21.15107/rcub_technorep_5939

Ilić N, Milić M, Davidović S, Mihajlovski K, Dimitrijević-Branković S. The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021. 2021;:158.
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme" in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021 (2021):158,
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

TY  - CONF
AU  - Filipović, Vladimir
AU  - Ugrenović, Vladan
AU  - Maksimović, Zoran
AU  - Dimitrijević, Snežana
AU  - Popović, Vera
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6424
AB  - This study has explored the effect of three phytohormones application, based on α-naphthyl acetic acid, named INCIT 1, INCIT 8 and INCIT K, on the root system growing potential and rooting stimulation of Thymus pannonicus All. cuttings. This plant is highly reputable not only in a folk medicine, but also recognized in the business sector, due to a multiple uses and beneficial effects on human health.
The research was located in Veliko Središte, Vršac (Serbia) and conducted in May, 2021. The cuttings were dipped in a powdered formulation of phytohormones and placed in plastic containers pre-filled with commercial substrate. The phytohormone-free variant was taken as a control. After 2 months, the cuttings were removed and the morphological and biological properties (polyphenols content and antioxidant activity by DPPH radical and FRAP method) were measured.
Based on the morphological results, all of treated samples showed satisfactory behaviour, whereby the most suitable phytohormone to stimulate the growth of cuttings was INCIT 8, in which the average weight of the aboveground vegetative part of the plant and root mass were higher for more than 60% in relation to the control; the number of roots were about 25% higher, whereby the mass of the whole plant was almost 3 times increased. Considering the biological activities in a 5% aqueous infusions of the samples, it was found that the polyphenols content and the antioxidant activity were lowered in treated samples, with relation to the control, excluding the sample INCIT 8 where the FRAP value was slightly increased, while the inhibition of DPPH radicals was higher by around 15%.
The applied phytohormone treatment may ensure economically viable yields of standard or even improved plant quality. In the following period, it is expected that all of treated adult plants will have a higher content of biologically active substances.
PB  - University of Belgrade
C3  - UNIFood Conference
T1  - The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6424
ER  - 

@conference{
author = "Filipović, Vladimir and Ugrenović, Vladan and Maksimović, Zoran and Dimitrijević, Snežana and Popović, Vera and Mihajlovski, Katarina and Milić, Marija",
year = "2021",
abstract = "This study has explored the effect of three phytohormones application, based on α-naphthyl acetic acid, named INCIT 1, INCIT 8 and INCIT K, on the root system growing potential and rooting stimulation of Thymus pannonicus All. cuttings. This plant is highly reputable not only in a folk medicine, but also recognized in the business sector, due to a multiple uses and beneficial effects on human health.
The research was located in Veliko Središte, Vršac (Serbia) and conducted in May, 2021. The cuttings were dipped in a powdered formulation of phytohormones and placed in plastic containers pre-filled with commercial substrate. The phytohormone-free variant was taken as a control. After 2 months, the cuttings were removed and the morphological and biological properties (polyphenols content and antioxidant activity by DPPH radical and FRAP method) were measured.
Based on the morphological results, all of treated samples showed satisfactory behaviour, whereby the most suitable phytohormone to stimulate the growth of cuttings was INCIT 8, in which the average weight of the aboveground vegetative part of the plant and root mass were higher for more than 60% in relation to the control; the number of roots were about 25% higher, whereby the mass of the whole plant was almost 3 times increased. Considering the biological activities in a 5% aqueous infusions of the samples, it was found that the polyphenols content and the antioxidant activity were lowered in treated samples, with relation to the control, excluding the sample INCIT 8 where the FRAP value was slightly increased, while the inhibition of DPPH radicals was higher by around 15%.
The applied phytohormone treatment may ensure economically viable yields of standard or even improved plant quality. In the following period, it is expected that all of treated adult plants will have a higher content of biologically active substances.",
publisher = "University of Belgrade",
journal = "UNIFood Conference",
title = "The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6424"
}

Filipović, V., Ugrenović, V., Maksimović, Z., Dimitrijević, S., Popović, V., Mihajlovski, K.,& Milić, M.. (2021). The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all. in UNIFood Conference
University of Belgrade., 151.
https://hdl.handle.net/21.15107/rcub_technorep_6424

Filipović V, Ugrenović V, Maksimović Z, Dimitrijević S, Popović V, Mihajlovski K, Milić M. The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all. in UNIFood Conference. 2021;:151.
https://hdl.handle.net/21.15107/rcub_technorep_6424 .

Filipović, Vladimir, Ugrenović, Vladan, Maksimović, Zoran, Dimitrijević, Snežana, Popović, Vera, Mihajlovski, Katarina, Milić, Marija, "The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all" in UNIFood Conference (2021):151,
https://hdl.handle.net/21.15107/rcub_technorep_6424 .

TY  - CONF
AU  - Buntić, Aneta
AU  - Tošić Jojević, Sonja
AU  - Knežević, Magdalena
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Koković, Nikola
AU  - Saljnikov, Elmira
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6426
AB  - The purpose of this study was to evaluate the effect of biomass ash from combustion of soybean straw
alone or in combination with bacterial inoculums on yield of barley crop, and on soil and plant quality.
The greenhouse pot experiment included four treatments and two control soils (control without any
amendment - ØØ and control with mineral fertilizer ØØ+CAN, 0.3g CAN/pot). Treatments included
soil (3 kg/pot) and biomass ash (30 g/pot) without and with one of three selected bacterial inoculums.
Bacterial strains Streptomyces fulvissimus CKS7, Hymenobacter sp. CKS3 and Sinorhizobium (Ensifer)
meliloti 207 were used for three inoculums preparation (CKS7, CKS7+CKS3 and 207). The ash and
experimental soil samples, as well as soil and plant samples collected at the stage of crop maturity
were tested for: total N, C, S, plant available P2O5 and K2O and for the content of potentially toxic and
hazardous microelements (As, Cd, Сo, Cr, Cu, Mn, Ni, Pb, Zn). The results showed that the addition of
biomass ash significantly reduced soil acidity and increased content of plant available P and K
compared to the controls (in all treatments). Barley yield was almost twice as higher in ash treatment
and by 1.56, 1.70 and 1.81 times higher in treatments Ash+CKS7, Ash+CKS7+CKS3 and Ash+207
respectively, compared to control (ØØ). The content of potentially toxic microelements was below the
maximum allowed concentrations in the soils and shoots. The use of combinations of ash and bacterial
inoculum improved the quality of barley in terms of nitrogen content. This study showed that biomass
ash can be used as fertilizer on acidic soils with low nutrient content alone or in combination with
applied inoculants and provide an environmentally friendly approach in agriculture to reduce the
need for chemical fertilizers and solve the problem of biomass ash disposal.
PB  - Federation of Eurasian Soil Science Societies
C3  - The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey
T1  - The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality
SP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6426
ER  - 

@conference{
author = "Buntić, Aneta and Tošić Jojević, Sonja and Knežević, Magdalena and Milić, Marija and Mihajlovski, Katarina and Koković, Nikola and Saljnikov, Elmira",
year = "2021",
abstract = "The purpose of this study was to evaluate the effect of biomass ash from combustion of soybean straw
alone or in combination with bacterial inoculums on yield of barley crop, and on soil and plant quality.
The greenhouse pot experiment included four treatments and two control soils (control without any
amendment - ØØ and control with mineral fertilizer ØØ+CAN, 0.3g CAN/pot). Treatments included
soil (3 kg/pot) and biomass ash (30 g/pot) without and with one of three selected bacterial inoculums.
Bacterial strains Streptomyces fulvissimus CKS7, Hymenobacter sp. CKS3 and Sinorhizobium (Ensifer)
meliloti 207 were used for three inoculums preparation (CKS7, CKS7+CKS3 and 207). The ash and
experimental soil samples, as well as soil and plant samples collected at the stage of crop maturity
were tested for: total N, C, S, plant available P2O5 and K2O and for the content of potentially toxic and
hazardous microelements (As, Cd, Сo, Cr, Cu, Mn, Ni, Pb, Zn). The results showed that the addition of
biomass ash significantly reduced soil acidity and increased content of plant available P and K
compared to the controls (in all treatments). Barley yield was almost twice as higher in ash treatment
and by 1.56, 1.70 and 1.81 times higher in treatments Ash+CKS7, Ash+CKS7+CKS3 and Ash+207
respectively, compared to control (ØØ). The content of potentially toxic microelements was below the
maximum allowed concentrations in the soils and shoots. The use of combinations of ash and bacterial
inoculum improved the quality of barley in terms of nitrogen content. This study showed that biomass
ash can be used as fertilizer on acidic soils with low nutrient content alone or in combination with
applied inoculants and provide an environmentally friendly approach in agriculture to reduce the
need for chemical fertilizers and solve the problem of biomass ash disposal.",
publisher = "Federation of Eurasian Soil Science Societies",
journal = "The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey",
title = "The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality",
pages = "22",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6426"
}

Buntić, A., Tošić Jojević, S., Knežević, M., Milić, M., Mihajlovski, K., Koković, N.,& Saljnikov, E.. (2021). The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality. in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey
Federation of Eurasian Soil Science Societies., 22.
https://hdl.handle.net/21.15107/rcub_technorep_6426

Buntić A, Tošić Jojević S, Knežević M, Milić M, Mihajlovski K, Koković N, Saljnikov E. The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality. in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey. 2021;:22.
https://hdl.handle.net/21.15107/rcub_technorep_6426 .

Buntić, Aneta, Tošić Jojević, Sonja, Knežević, Magdalena, Milić, Marija, Mihajlovski, Katarina, Koković, Nikola, Saljnikov, Elmira, "The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality" in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey (2021):22,
https://hdl.handle.net/21.15107/rcub_technorep_6426 .

TY  - CONF
AU  - Radovanović, Željko
AU  - Mihajlovski, Katarina
AU  - Radovanović, Lidija
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7399
AB  - The synergistic effect of two materials is a desirable phenomenon when designing a conjugate
material. Synthetic Hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is very similar to the inorganic part of
bones and teeth of mammals. It is a well-known biomaterial with good biocompatibility,
osteoconductivity and bioactivity. Nifuroxazide (NFX, C12H9N3O5) is a broad-spectrum antibacterial
drug and poorly soluble in water. Nano HAp and NFX were mixed and homogenized in rotovapor
giving as a result HAp/NFX conjugate. All materials were analyzed by Fourier transform infrared
spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), X-Ray diffraction
(XRD), Thermogravimetry (TG) and Brunauer–Emmett–Teller (BET) method. The in vitro
antibacterial activities of HAp, NFX and HAp/NFX were examined against Gram-positive bacteria
Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans. In vitro
drug release in simulated stomach acid and intestinal fluid showed a much faster release of NFX from
HAp surface than those of raw drug. Afore mentioned analyses showed the unique properties of the
HAp/NFX conjugate and introduced it as particularly prominent drug carrier.
PB  - Belgrade : Materials Research Society of Serbia
C3  - Programme and the Book of abstracts / Twenty-second Annual Conference YUCOMAT 2021 Herceg Novi, Montenegro, August 30 - September 3, 2021
T1  - Promising Hydroxyapatite/Nifuroksazid Conjugate
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7399
ER  - 

@conference{
author = "Radovanović, Željko and Mihajlovski, Katarina and Radovanović, Lidija and Petrović, Rada and Janaćković, Đorđe",
year = "2021",
abstract = "The synergistic effect of two materials is a desirable phenomenon when designing a conjugate
material. Synthetic Hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is very similar to the inorganic part of
bones and teeth of mammals. It is a well-known biomaterial with good biocompatibility,
osteoconductivity and bioactivity. Nifuroxazide (NFX, C12H9N3O5) is a broad-spectrum antibacterial
drug and poorly soluble in water. Nano HAp and NFX were mixed and homogenized in rotovapor
giving as a result HAp/NFX conjugate. All materials were analyzed by Fourier transform infrared
spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), X-Ray diffraction
(XRD), Thermogravimetry (TG) and Brunauer–Emmett–Teller (BET) method. The in vitro
antibacterial activities of HAp, NFX and HAp/NFX were examined against Gram-positive bacteria
Staphylococcus aureus, Gram-negative bacteria Escherichia coli and yeast Candida albicans. In vitro
drug release in simulated stomach acid and intestinal fluid showed a much faster release of NFX from
HAp surface than those of raw drug. Afore mentioned analyses showed the unique properties of the
HAp/NFX conjugate and introduced it as particularly prominent drug carrier.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "Programme and the Book of abstracts / Twenty-second Annual Conference YUCOMAT 2021 Herceg Novi, Montenegro, August 30 - September 3, 2021",
title = "Promising Hydroxyapatite/Nifuroksazid Conjugate",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7399"
}

Radovanović, Ž., Mihajlovski, K., Radovanović, L., Petrović, R.,& Janaćković, Đ.. (2021). Promising Hydroxyapatite/Nifuroksazid Conjugate. in Programme and the Book of abstracts / Twenty-second Annual Conference YUCOMAT 2021 Herceg Novi, Montenegro, August 30 - September 3, 2021
Belgrade : Materials Research Society of Serbia., 126.
https://hdl.handle.net/21.15107/rcub_technorep_7399

Radovanović Ž, Mihajlovski K, Radovanović L, Petrović R, Janaćković Đ. Promising Hydroxyapatite/Nifuroksazid Conjugate. in Programme and the Book of abstracts / Twenty-second Annual Conference YUCOMAT 2021 Herceg Novi, Montenegro, August 30 - September 3, 2021. 2021;:126.
https://hdl.handle.net/21.15107/rcub_technorep_7399 .

Radovanović, Željko, Mihajlovski, Katarina, Radovanović, Lidija, Petrović, Rada, Janaćković, Đorđe, "Promising Hydroxyapatite/Nifuroksazid Conjugate" in Programme and the Book of abstracts / Twenty-second Annual Conference YUCOMAT 2021 Herceg Novi, Montenegro, August 30 - September 3, 2021 (2021):126,
https://hdl.handle.net/21.15107/rcub_technorep_7399 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Lazić, Vesna
AU  - Radovanović, Neda
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana
AU  - Miljković, Miona
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5927
AB  - Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean
EP  - 460
SP  - 451
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5927
ER  - 

@conference{
author = "Ilić, Nevena and Lazić, Vesna and Radovanović, Neda and Mihajlovski, Katarina and Davidović, Slađana and Miljković, Miona",
year = "2021",
abstract = "Silver and titanium dioxide nanoparticles (NPs) are the most produced NPs worldwide due to their antimicrobial properties. These NPs are widely used in many fields including agriculture, where they can inhibit the growth of plant pathogens and stimulate plant growth. However, there are concerns about nanoparticles reaching the environment, as they can also manifest adverse effects. In this study, the effects of TiO2 and two types of Ag NPs (synthesized by chemical reduction and “green” by dextrane) on soil microorganisms and organic beans were investigated. Two different species of rhizospheric soil microorganisms, namely actinomycetes Streptomyces microflavus and nitrogen-fixing soil bacteria Rhizobium sp. were exposed to the low concentration of NPs. For that purpose, microorganisms were grown in nutrient media supplemented with 0.2 mM NPs. After 24 h of exposure, the numbers of viable cells of both tested microorganisms were higher in the samples compared to the controls (media without NPs) for all three NPs, suggesting their stimulatory effect on the soil microorganisms' growth. The highest positive effect was observed for Rhizobium sp. treated with TiO2 NPs (the number of viable cells increased for two orders of magnitude, from ~106 to ~108 CFU/mL). Ag NPs showed equal influence on both tested microorganisms, with dextrane-coated Ag NPs were more effective. In the experiment with organic mung beans, the same colloid solutions of Ag NPs and TiO2 NPs had a stimulating effect on the germination and root length of this plant, also. The number of germinated seeds was the same as in the control, while the average root length was higher in the samples than in the control. The average root length was 1.47 cm in the control. Seeds treated with dextrane-coated Ag NPs had an average root length even 82 % higher compared to the control. The stimulatory effect was also observed in the case of seeds treated with Ag NPs synthesized by chemical reduction and TiO2 NPs, where the average root lengths were higher for 40% and 76 %, respectively than in the control. Germination index values, which are higher than 100%, suggesting that tested nanoparticles can be used as fertilizers in agriculture. Moreover, the obtained results show that silver nanoparticles and titanium dioxide nanoparticles have a stimulatory effect on the soil bacteria and organic mung bean, which prove their safety for this kind of application.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean",
pages = "460-451",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5927"
}

Ilić, N., Lazić, V., Radovanović, N., Mihajlovski, K., Davidović, S.,& Miljković, M.. (2021). Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927

Ilić N, Lazić V, Radovanović N, Mihajlovski K, Davidović S, Miljković M. Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:451-460.
https://hdl.handle.net/21.15107/rcub_technorep_5927 .

Ilić, Nevena, Lazić, Vesna, Radovanović, Neda, Mihajlovski, Katarina, Davidović, Slađana, Miljković, Miona, "Investigation of the influence of different nanoparticles on the growth of soil microorganisms and organic mung bean" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):451-460,
https://hdl.handle.net/21.15107/rcub_technorep_5927 .