TY  - JOUR
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Dinić, Ivana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar
AU  - Costa, Antonio Mario Leal Martins
AU  - Marinković, Bojan A.
AU  - Mojović, Ljiljana
AU  - Milošević, Olivera
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3885
AB  - The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt  (4)/(15/2)) and stronger red emission (F-4(9/2) - gt  (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt  (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging
EP  - 27437
IS  - 48
SP  - 27429
VL  - 8
DO  - 10.1039/c8ra04178d
ER  - 

@article{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Marinković, Bojan A. and Mojović, Ljiljana and Milošević, Olivera",
year = "2018",
abstract = "The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic alpha phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (H-2(11/2), S-4(3/2) - gt  (4)/(15/2)) and stronger red emission (F-4(9/2) - gt  (4)/(15/2)), as a result of enhanced non-radiative (4)/(11/2) - gt  (4)/(13/2) Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 mu g ml(-1), without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging",
pages = "27437-27429",
number = "48",
volume = "8",
doi = "10.1039/c8ra04178d"
}

Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Marinković, B. A., Mojović, L.,& Milošević, O.. (2018). One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances
Royal Society of Chemistry., 8(48), 27429-27437.
https://doi.org/10.1039/c8ra04178d

Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Marinković BA, Mojović L, Milošević O. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. in RSC Advances. 2018;8(48):27429-27437.
doi:10.1039/c8ra04178d .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Marinković, Bojan A., Mojović, Ljiljana, Milošević, Olivera, "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging" in RSC Advances, 8, no. 48 (2018):27429-27437,
https://doi.org/10.1039/c8ra04178d . .

TY  - DATA
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Dinić, Ivana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar
AU  - Costa, Antonio Mario Leal Martins
AU  - Marinković, Bojan A.
AU  - Mojović, Ljiljana
AU  - Milošević, Olivera
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3772
AB  - Fig. S1. TEM (a) and HRTEM (b) images of amino modified NaYF4:Yb,Er UCNPs. Corresponding FFT/IFFT given as insets in b, confirms that much smaller crystallites notable at the particles surfaces (a) revealed periodic array of cubic alpha phase, (111) plane with d value of 3.140 Å; Fig. S2. XPS spectrum of amino-functionalized NaYF4:Yb,Er UCNPs: survey spectrum and fine-scan spectra of Na 1s, Y 3d, Yb 4d, Er 4d, F 1s and decomposed ones of C 1s, O 1s and N 1s; Fig. S3. Photostability of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles. The emission intensity was traced during 1h;
PB  - Royal Society of Chemistry
T2  - RSC Advances
T1  - Supplementary file: Mancic, L., Djukic-Vukovic, A., Dinic, I., Nikolic, M.G., Rabasovic, M.D., Krmpot, A.J., Costa, A.M.L.M., Marinkovic, B.A., Mojovic, L., Milosevic, O., 2018. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. RSC Adv. 8, 27429–27437. https://doi.org/10.1039/C8RA04178D
IS  - 48
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_dais_3785
ER  - 

@misc{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Marinković, Bojan A. and Mojović, Ljiljana and Milošević, Olivera",
year = "2018",
abstract = "Fig. S1. TEM (a) and HRTEM (b) images of amino modified NaYF4:Yb,Er UCNPs. Corresponding FFT/IFFT given as insets in b, confirms that much smaller crystallites notable at the particles surfaces (a) revealed periodic array of cubic alpha phase, (111) plane with d value of 3.140 Å; Fig. S2. XPS spectrum of amino-functionalized NaYF4:Yb,Er UCNPs: survey spectrum and fine-scan spectra of Na 1s, Y 3d, Yb 4d, Er 4d, F 1s and decomposed ones of C 1s, O 1s and N 1s; Fig. S3. Photostability of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles. The emission intensity was traced during 1h;",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances",
title = "Supplementary file: Mancic, L., Djukic-Vukovic, A., Dinic, I., Nikolic, M.G., Rabasovic, M.D., Krmpot, A.J., Costa, A.M.L.M., Marinkovic, B.A., Mojovic, L., Milosevic, O., 2018. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. RSC Adv. 8, 27429–27437. https://doi.org/10.1039/C8RA04178D",
number = "48",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_dais_3785"
}

Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Marinković, B. A., Mojović, L.,& Milošević, O.. (2018). Supplementary file: Mancic, L., Djukic-Vukovic, A., Dinic, I., Nikolic, M.G., Rabasovic, M.D., Krmpot, A.J., Costa, A.M.L.M., Marinkovic, B.A., Mojovic, L., Milosevic, O., 2018. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. RSC Adv. 8, 27429–27437. https://doi.org/10.1039/C8RA04178D. in RSC Advances
Royal Society of Chemistry., 8(48).
https://hdl.handle.net/21.15107/rcub_dais_3785

Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Marinković BA, Mojović L, Milošević O. Supplementary file: Mancic, L., Djukic-Vukovic, A., Dinic, I., Nikolic, M.G., Rabasovic, M.D., Krmpot, A.J., Costa, A.M.L.M., Marinkovic, B.A., Mojovic, L., Milosevic, O., 2018. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. RSC Adv. 8, 27429–27437. https://doi.org/10.1039/C8RA04178D. in RSC Advances. 2018;8(48).
https://hdl.handle.net/21.15107/rcub_dais_3785 .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Marinković, Bojan A., Mojović, Ljiljana, Milošević, Olivera, "Supplementary file: Mancic, L., Djukic-Vukovic, A., Dinic, I., Nikolic, M.G., Rabasovic, M.D., Krmpot, A.J., Costa, A.M.L.M., Marinkovic, B.A., Mojovic, L., Milosevic, O., 2018. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging. RSC Adv. 8, 27429–27437. https://doi.org/10.1039/C8RA04178D" in RSC Advances, 8, no. 48 (2018),
https://hdl.handle.net/21.15107/rcub_dais_3785 .

TY  - DATA
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Dinić, Ivana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar
AU  - Costa, Antonio Mario Leal Martins
AU  - Trišić, Dijana
AU  - Lazarević, Miloš
AU  - Mojović, Ljiljana
AU  - Milošević, Olivera B.
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3769
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science and Engineering. C: Materials for Biological Applications
T1  - Supplementary material for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.;  Costa, A. M. L. M.; Trisic, D.; Lazarevic, M.; Mojovic, L.; et al. NIR Photo-Driven  Upconversion in NaYF 4 :Yb,Er/PLGA Particles for in Vitro Bioimaging of Cancer Cells.  Materials Science and Engineering C 2018, 91, 597–605.  https://doi.org/10.1016/j.msec.2018.05.081
UR  - https://hdl.handle.net/21.15107/rcub_dais_5978
ER  - 

@misc{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Trišić, Dijana and Lazarević, Miloš and Mojović, Ljiljana and Milošević, Olivera B.",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science and Engineering. C: Materials for Biological Applications",
title = "Supplementary material for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.;  Costa, A. M. L. M.; Trisic, D.; Lazarevic, M.; Mojovic, L.; et al. NIR Photo-Driven  Upconversion in NaYF 4 :Yb,Er/PLGA Particles for in Vitro Bioimaging of Cancer Cells.  Materials Science and Engineering C 2018, 91, 597–605.  https://doi.org/10.1016/j.msec.2018.05.081",
url = "https://hdl.handle.net/21.15107/rcub_dais_5978"
}

Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Trišić, D., Lazarević, M., Mojović, L.,& Milošević, O. B.. (2018). Supplementary material for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.;  Costa, A. M. L. M.; Trisic, D.; Lazarevic, M.; Mojovic, L.; et al. NIR Photo-Driven  Upconversion in NaYF 4 :Yb,Er/PLGA Particles for in Vitro Bioimaging of Cancer Cells.  Materials Science and Engineering C 2018, 91, 597–605.  https://doi.org/10.1016/j.msec.2018.05.081. in Materials Science and Engineering. C: Materials for Biological Applications
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_dais_5978

Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Trišić D, Lazarević M, Mojović L, Milošević OB. Supplementary material for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.;  Costa, A. M. L. M.; Trisic, D.; Lazarevic, M.; Mojovic, L.; et al. NIR Photo-Driven  Upconversion in NaYF 4 :Yb,Er/PLGA Particles for in Vitro Bioimaging of Cancer Cells.  Materials Science and Engineering C 2018, 91, 597–605.  https://doi.org/10.1016/j.msec.2018.05.081. in Materials Science and Engineering. C: Materials for Biological Applications. 2018;.
https://hdl.handle.net/21.15107/rcub_dais_5978 .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Trišić, Dijana, Lazarević, Miloš, Mojović, Ljiljana, Milošević, Olivera B., "Supplementary material for the article: Mancic, L.; Djukic-Vukovic, A.; Dinic, I.; Nikolic, M. G.; Rabasovic, M. D.; Krmpot, A. J.;  Costa, A. M. L. M.; Trisic, D.; Lazarevic, M.; Mojovic, L.; et al. NIR Photo-Driven  Upconversion in NaYF 4 :Yb,Er/PLGA Particles for in Vitro Bioimaging of Cancer Cells.  Materials Science and Engineering C 2018, 91, 597–605.  https://doi.org/10.1016/j.msec.2018.05.081" in Materials Science and Engineering. C: Materials for Biological Applications (2018),
https://hdl.handle.net/21.15107/rcub_dais_5978 .

TY  - JOUR
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Dinić, Ivana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar
AU  - Costa, Antonio Mario Leal Martins
AU  - Trišić, Dijana
AU  - Lazarević, Miloš
AU  - Mojović, Ljiljana
AU  - Milošević, Olivera
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3852
AB  - Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science & Engineering C-Materials for Biological Applications
T1  - NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells
EP  - 605
SP  - 597
VL  - 91
DO  - 10.1016/j.msec.2018.05.081
ER  - 

@article{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Trišić, Dijana and Lazarević, Miloš and Mojović, Ljiljana and Milošević, Olivera",
year = "2018",
abstract = "Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P6(3)/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (lambda(ex) = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10-50 mu g/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science & Engineering C-Materials for Biological Applications",
title = "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells",
pages = "605-597",
volume = "91",
doi = "10.1016/j.msec.2018.05.081"
}

Mančić, L., Đukić-Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Trišić, D., Lazarević, M., Mojović, L.,& Milošević, O.. (2018). NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science & Engineering C-Materials for Biological Applications
Elsevier Science Bv, Amsterdam., 91, 597-605.
https://doi.org/10.1016/j.msec.2018.05.081

Mančić L, Đukić-Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Trišić D, Lazarević M, Mojović L, Milošević O. NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science & Engineering C-Materials for Biological Applications. 2018;91:597-605.
doi:10.1016/j.msec.2018.05.081 .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Trišić, Dijana, Lazarević, Miloš, Mojović, Ljiljana, Milošević, Olivera, "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells" in Materials Science & Engineering C-Materials for Biological Applications, 91 (2018):597-605,
https://doi.org/10.1016/j.msec.2018.05.081 . .

TY  - CONF
AU  - Dinić, Ivana
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Nikolić, Marko G.
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar
AU  - Milošević, Olivera
AU  - Mančić, Lidija
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3486
AB  - There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles with specific morphological characteristics and efficient luminescence response suitable for biomedical use [1]. A conventional approach for generation of such particles comprises decomposition of organometallic compoundsin an oxygen-free environment and additional ligand exchange [2,3]. The biocompatible and water soluble NaYF4:Yb,Er"Chitosane particles used in this study were synthesized through facile one-pot hydrothermal synthesis and were characterized using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Due to the presence of the amino groups at their surface these particles exhibit excellent hydrophilic properties and low cytotoxicity against human gingival fibroblasts (HGF), which was proven by MTT assay. Furthermore, upon 980 nm laser irradiation the as-prepared particles were successfully used for in-vitro visualization of the primary cell cultures of head and neck squamous carcinoma cells (HNSCC). In a NaYF4:Yb,Er phase upconversion is enabled by the sequential absorption of two or more near-infrared photons by Yb3+ and subsequent energy transfer to the long-lived metastable electron states of Er3+ which produces luminescence emission at visible spectra after relaxation.
PB  - Belgrade : Institute of Physics Belgrade
C3  - Abstracts of Tutorial, Keynote, Invited Lectures, Progress Reports and Contributed Papers of the Six
T1  - One-step synthesis of NIR-responsive NaYF4:Yb,Er"Chitosane nanoparticles for biomedical application
EP  - 81
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3486
ER  - 

@conference{
author = "Dinić, Ivana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Milošević, Olivera and Mančić, Lidija",
year = "2017",
abstract = "There is a great technological interest in synthesis of lanthanide doped upconverting nanoparticles with specific morphological characteristics and efficient luminescence response suitable for biomedical use [1]. A conventional approach for generation of such particles comprises decomposition of organometallic compoundsin an oxygen-free environment and additional ligand exchange [2,3]. The biocompatible and water soluble NaYF4:Yb,Er"Chitosane particles used in this study were synthesized through facile one-pot hydrothermal synthesis and were characterized using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Due to the presence of the amino groups at their surface these particles exhibit excellent hydrophilic properties and low cytotoxicity against human gingival fibroblasts (HGF), which was proven by MTT assay. Furthermore, upon 980 nm laser irradiation the as-prepared particles were successfully used for in-vitro visualization of the primary cell cultures of head and neck squamous carcinoma cells (HNSCC). In a NaYF4:Yb,Er phase upconversion is enabled by the sequential absorption of two or more near-infrared photons by Yb3+ and subsequent energy transfer to the long-lived metastable electron states of Er3+ which produces luminescence emission at visible spectra after relaxation.",
publisher = "Belgrade : Institute of Physics Belgrade",
journal = "Abstracts of Tutorial, Keynote, Invited Lectures, Progress Reports and Contributed Papers of the Six",
title = "One-step synthesis of NIR-responsive NaYF4:Yb,Er"Chitosane nanoparticles for biomedical application",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3486"
}

Dinić, I., Đukić-Vuković, A., Mojović, L., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Milošević, O.,& Mančić, L.. (2017). One-step synthesis of NIR-responsive NaYF4:Yb,Er"Chitosane nanoparticles for biomedical application. in Abstracts of Tutorial, Keynote, Invited Lectures, Progress Reports and Contributed Papers of the Six
Belgrade : Institute of Physics Belgrade., 81-81.
https://hdl.handle.net/21.15107/rcub_technorep_3486

Dinić I, Đukić-Vuković A, Mojović L, Nikolić MG, Rabasović MD, Krmpot A, Milošević O, Mančić L. One-step synthesis of NIR-responsive NaYF4:Yb,Er"Chitosane nanoparticles for biomedical application. in Abstracts of Tutorial, Keynote, Invited Lectures, Progress Reports and Contributed Papers of the Six. 2017;:81-81.
https://hdl.handle.net/21.15107/rcub_technorep_3486 .

Dinić, Ivana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Milošević, Olivera, Mančić, Lidija, "One-step synthesis of NIR-responsive NaYF4:Yb,Er"Chitosane nanoparticles for biomedical application" in Abstracts of Tutorial, Keynote, Invited Lectures, Progress Reports and Contributed Papers of the Six (2017):81-81,
https://hdl.handle.net/21.15107/rcub_technorep_3486 .

TY  - CONF
AU  - Mančić, Lidija
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Rabasović, Mihailo D.
AU  - Krmpot, Aleksandar
AU  - Dinić, Ivana
AU  - Costa, Antonio Mario Leal Martins
AU  - Milošević, Olivera
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3488
AB  - For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er"PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.
PB  - Belgrade : Serbian Ceramic Society
C3  - Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica
T1  - In-vitro visualization of primary tumor cells using up-conversion nanophosphors
EP  - 59
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3488
ER  - 

@conference{
author = "Mančić, Lidija and Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Rabasović, Mihailo D. and Krmpot, Aleksandar and Dinić, Ivana and Costa, Antonio Mario Leal Martins and Milošević, Olivera",
year = "2017",
abstract = "For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er"PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica",
title = "In-vitro visualization of primary tumor cells using up-conversion nanophosphors",
pages = "59-59",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3488"
}

Mančić, L., Đukić-Vuković, A., Mojović, L., Rabasović, M. D., Krmpot, A., Dinić, I., Costa, A. M. L. M.,& Milošević, O.. (2017). In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica
Belgrade : Serbian Ceramic Society., 59-59.
https://hdl.handle.net/21.15107/rcub_technorep_3488

Mančić L, Đukić-Vuković A, Mojović L, Rabasović MD, Krmpot A, Dinić I, Costa AMLM, Milošević O. In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica. 2017;:59-59.
https://hdl.handle.net/21.15107/rcub_technorep_3488 .

Mančić, Lidija, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Rabasović, Mihailo D., Krmpot, Aleksandar, Dinić, Ivana, Costa, Antonio Mario Leal Martins, Milošević, Olivera, "In-vitro visualization of primary tumor cells using up-conversion nanophosphors" in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Applica (2017):59-59,
https://hdl.handle.net/21.15107/rcub_technorep_3488 .

TY  - CONF
AU  - Dinić, Ivana
AU  - Đukić-Vuković, Aleksandra
AU  - Nikolić, Marko G.
AU  - Milošević, Olivera
AU  - Mančić, Lidija
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3492
AB  - Lanthanide doped up-converting nanoparticles (UCNPs) have significant role in biomedical field, especially in cell imaging and target drug delivery, due to their convenient luminescent properties. For that purpose UCNPs should have the specific morphological and luminescent characteristics. In this study the biocompatible NaYF4:Yb,Er"Chitosane particles were synthesized through one-step hydrothermal synthesis. Obtained particles were characterized in detail using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Structural refinement data show the presence of cubic (Fm-3m) phase in spherical shaped nanoparticles size up to 200 nm, while the FTIR analysis revealed the presence of chitosan on the particle surface which have no influence on the luminescence efficiency of the UCNPs. Cytotoxicity as well as cell labeling capability of synthesized UCNPs were tested in vitro on the human gingival fibroblasts (HGF) and head and neck squamous carcinoma cells (HNSCC). The results show excellent biocompatibility against HGF, and successful in- vitro visualization of HNSCC cell cultures upon 980 nm laser irradiation.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and E
T1  - Photo-driven upconversion in NaYF4:Yb,Er"chitosane particles for cancer cells bioimaging
EP  - 2
SP  - 2
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3492
ER  - 

@conference{
author = "Dinić, Ivana and Đukić-Vuković, Aleksandra and Nikolić, Marko G. and Milošević, Olivera and Mančić, Lidija",
year = "2017",
abstract = "Lanthanide doped up-converting nanoparticles (UCNPs) have significant role in biomedical field, especially in cell imaging and target drug delivery, due to their convenient luminescent properties. For that purpose UCNPs should have the specific morphological and luminescent characteristics. In this study the biocompatible NaYF4:Yb,Er"Chitosane particles were synthesized through one-step hydrothermal synthesis. Obtained particles were characterized in detail using X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, field emission scanning and transmission electron microscopy (FESEM and TEM) and photoluminesce measurement (PL). Structural refinement data show the presence of cubic (Fm-3m) phase in spherical shaped nanoparticles size up to 200 nm, while the FTIR analysis revealed the presence of chitosan on the particle surface which have no influence on the luminescence efficiency of the UCNPs. Cytotoxicity as well as cell labeling capability of synthesized UCNPs were tested in vitro on the human gingival fibroblasts (HGF) and head and neck squamous carcinoma cells (HNSCC). The results show excellent biocompatibility against HGF, and successful in- vitro visualization of HNSCC cell cultures upon 980 nm laser irradiation.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and E",
title = "Photo-driven upconversion in NaYF4:Yb,Er"chitosane particles for cancer cells bioimaging",
pages = "2-2",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3492"
}

Dinić, I., Đukić-Vuković, A., Nikolić, M. G., Milošević, O.,& Mančić, L.. (2017). Photo-driven upconversion in NaYF4:Yb,Er"chitosane particles for cancer cells bioimaging. in Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and E
Belgrade : Institute of Technical Sciences of SASA., 2-2.
https://hdl.handle.net/21.15107/rcub_technorep_3492

Dinić I, Đukić-Vuković A, Nikolić MG, Milošević O, Mančić L. Photo-driven upconversion in NaYF4:Yb,Er"chitosane particles for cancer cells bioimaging. in Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and E. 2017;:2-2.
https://hdl.handle.net/21.15107/rcub_technorep_3492 .

Dinić, Ivana, Đukić-Vuković, Aleksandra, Nikolić, Marko G., Milošević, Olivera, Mančić, Lidija, "Photo-driven upconversion in NaYF4:Yb,Er"chitosane particles for cancer cells bioimaging" in Program and the Book of Abstracts / Sixteenth Young Researchers' Conference Materials Sciences and E (2017):2-2,
https://hdl.handle.net/21.15107/rcub_technorep_3492 .

TY  - CONF
AU  - Milisavljević, Ana
AU  - Stojanović, Marija
AU  - Dinić, Ivana
AU  - Carević, Milica
AU  - Mihailović, Mladen
AU  - Milosavić, Nenad
AU  - Bezbradica, Dejan
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6070
AB  - Phloridzin is member of chemical class of dihydrochalcones, phenylpropanoids with
structure similar to chalcones, immediate flavonoid precursors, hence it is often classified
as flavonoid glucoside [1,2]. It is usually extracted from Malus species since it is very
abundant in young apple leaves and twigs. Due to its phenolic structure, phloridzin has
significant antioxidant activity and anti-UV properties, which makes it interesting for
application in food and cosmetics. Major limitation to wider application of phloridzin is its
low solubility in hydrophobic environment, which can be circumvented by synthesis of
physiologically active compounds derivatives by acylation of phloridzin. Synthesis of acyl
esters can be catalyzed by inorganic catalysts or enzymes, but chemical esterification is
not regioselective and results with unwanted functionalization of phenolic hydroxyl
groups responsible for antioxidative properties.
Therefore, in our study enzymatic esterification of phloridzin was performed using
immobilized lipase from Candida antarctica (Novozyme® 435). Several organic solvents
were tested and acetonitrile was proved to be the most adequate medium for this
reaction. Different acyl-donors were used with respect to chain length and saturation
level. Potential physiological activity of obtained esters was evaluated by determination of
their antioxidant activity using DPPH assay, so acyl donors were compared with respect to
both, product yields and antioxidant activity. After comparison of results of preliminary
study, phloridzyl oleate was selected as derivative with the best prospects and it used in
further experimental series for optimization of key experimental factors. Response surface
methodology was applied as statistical tool for optimization of product concentration (in
mM) as output and analyzed factors were: reaction time, temperature, enzyme
concentration, substrate molar ratio, and phloridzin concentration. After statistical
analysis each of examined experimental factors was found significant and second order
regression model was obtained. It was established that highest product concentration can
be expected at 68 oC, with 0.17 M of phloridzin, 2,5 % (w/v) of enzyme, 19-fold molar
excess of oleic acid after 7 days of reaction.
C3  - 8th International Conference of the Chemical Societies of the South-East European Countries, Belgrade, Serbia
T1  - Lipase-catalyzed synthesis of phloridzin esters
SP  - 254
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6070
ER  - 

@conference{
author = "Milisavljević, Ana and Stojanović, Marija and Dinić, Ivana and Carević, Milica and Mihailović, Mladen and Milosavić, Nenad and Bezbradica, Dejan",
year = "2013",
abstract = "Phloridzin is member of chemical class of dihydrochalcones, phenylpropanoids with
structure similar to chalcones, immediate flavonoid precursors, hence it is often classified
as flavonoid glucoside [1,2]. It is usually extracted from Malus species since it is very
abundant in young apple leaves and twigs. Due to its phenolic structure, phloridzin has
significant antioxidant activity and anti-UV properties, which makes it interesting for
application in food and cosmetics. Major limitation to wider application of phloridzin is its
low solubility in hydrophobic environment, which can be circumvented by synthesis of
physiologically active compounds derivatives by acylation of phloridzin. Synthesis of acyl
esters can be catalyzed by inorganic catalysts or enzymes, but chemical esterification is
not regioselective and results with unwanted functionalization of phenolic hydroxyl
groups responsible for antioxidative properties.
Therefore, in our study enzymatic esterification of phloridzin was performed using
immobilized lipase from Candida antarctica (Novozyme® 435). Several organic solvents
were tested and acetonitrile was proved to be the most adequate medium for this
reaction. Different acyl-donors were used with respect to chain length and saturation
level. Potential physiological activity of obtained esters was evaluated by determination of
their antioxidant activity using DPPH assay, so acyl donors were compared with respect to
both, product yields and antioxidant activity. After comparison of results of preliminary
study, phloridzyl oleate was selected as derivative with the best prospects and it used in
further experimental series for optimization of key experimental factors. Response surface
methodology was applied as statistical tool for optimization of product concentration (in
mM) as output and analyzed factors were: reaction time, temperature, enzyme
concentration, substrate molar ratio, and phloridzin concentration. After statistical
analysis each of examined experimental factors was found significant and second order
regression model was obtained. It was established that highest product concentration can
be expected at 68 oC, with 0.17 M of phloridzin, 2,5 % (w/v) of enzyme, 19-fold molar
excess of oleic acid after 7 days of reaction.",
journal = "8th International Conference of the Chemical Societies of the South-East European Countries, Belgrade, Serbia",
title = "Lipase-catalyzed synthesis of phloridzin esters",
pages = "254",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6070"
}

Milisavljević, A., Stojanović, M., Dinić, I., Carević, M., Mihailović, M., Milosavić, N.,& Bezbradica, D.. (2013). Lipase-catalyzed synthesis of phloridzin esters. in 8th International Conference of the Chemical Societies of the South-East European Countries, Belgrade, Serbia, 254.
https://hdl.handle.net/21.15107/rcub_technorep_6070

Milisavljević A, Stojanović M, Dinić I, Carević M, Mihailović M, Milosavić N, Bezbradica D. Lipase-catalyzed synthesis of phloridzin esters. in 8th International Conference of the Chemical Societies of the South-East European Countries, Belgrade, Serbia. 2013;:254.
https://hdl.handle.net/21.15107/rcub_technorep_6070 .

Milisavljević, Ana, Stojanović, Marija, Dinić, Ivana, Carević, Milica, Mihailović, Mladen, Milosavić, Nenad, Bezbradica, Dejan, "Lipase-catalyzed synthesis of phloridzin esters" in 8th International Conference of the Chemical Societies of the South-East European Countries, Belgrade, Serbia (2013):254,
https://hdl.handle.net/21.15107/rcub_technorep_6070 .