TY  - PAT
AU  - Svetozarević Arsović, Milica
AU  - Šekuljica, Nataša
AU  - Stupar, Stevan
AU  - Nedeljkov, Tanja
AU  - Ivanovska, Aleksandra
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6970
T2  - Zavod za intelektualnu svojinu republike Srbije
T1  - Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6970
ER  - 

@misc{
author = "Svetozarević Arsović, Milica and Šekuljica, Nataša and Stupar, Stevan and Nedeljkov, Tanja and Ivanovska, Aleksandra and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2023",
journal = "Zavod za intelektualnu svojinu republike Srbije",
title = "Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6970"
}

Svetozarević Arsović, M., Šekuljica, N., Stupar, S., Nedeljkov, T., Ivanovska, A., Knežević-Jugović, Z.,& Mijin, D.. (2023). Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira. in Zavod za intelektualnu svojinu republike Srbije.
https://hdl.handle.net/21.15107/rcub_technorep_6970

Svetozarević Arsović M, Šekuljica N, Stupar S, Nedeljkov T, Ivanovska A, Knežević-Jugović Z, Mijin D. Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira. in Zavod za intelektualnu svojinu republike Srbije. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6970 .

Svetozarević Arsović, Milica, Šekuljica, Nataša, Stupar, Stevan, Nedeljkov, Tanja, Ivanovska, Aleksandra, Knežević-Jugović, Zorica, Mijin, Dušan, "Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira" in Zavod za intelektualnu svojinu republike Srbije (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6970 .

TY  - CONF
AU  - Nedeljkov, Tanja
AU  - Svetozarević Arsović, Milica
AU  - Šekuljica, Nataša
AU  - Mijin, Dušan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7034
AB  - Various strategies have been developed for the removal of synthetic dyes from the
wastewater of the textile industry due to their stability as well as their negative effect on the
environment. Enzymatic wastewater treatments as environmentally safe processes have many
benefits. An enzyme suitable for use in the treatment of wastewater from the textile industry
is horseradish peroxidase (HRP) because it can degrade different types of dyes. Immobilized
enzymes have many advantages over free enzymes in solution such as higher stability and the
opportunity to be removed easily from the reaction mixtures. Consequently, immobilized
enzymes can be used more than once. Many different materials are used as a carrier for
enzyme immobilization, so if residual material is used as a carrier, waste valorization will be
achieved. In this study immobilized commercial HRP was used for decolorization of
anthraquinone dye Acid green 40 (AG 40). Waste material – potato peels were used as a
carrier for the immobilization of HRP. Potato peels were oxidized with sodium periodate to
introduce aldehyde groups that can react with the primary amino groups of the enzyme to
form Schiff bases, resulting in covalently immobilized HRP. The success of the oxidation
was verified by the 2,4-dinitrophenylhydrazine (DNPH) test, and subsequently, the oxidized
potato peels were characterized by SEM and FTIR. After oxidation, the influence of the mass
of the immobilization carrier on enzyme activity and immobilization efficiency was
investigated. The optimal conditions of pH, hydrogen peroxide concentration, concentration
of AG 40, and enzyme activity were determined for decolorization of AG 40 with
immobilized HRP. Adsorption of AG 40 on the carrier was also determined. In addition, the
operational stability of the enzyme was determined. The results obtained show that oxidized
potato peels are a promising material that can be used as a carrier for the immobilization of
HRP.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023
T1  - New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7034
ER  - 

@conference{
author = "Nedeljkov, Tanja and Svetozarević Arsović, Milica and Šekuljica, Nataša and Mijin, Dušan",
year = "2023",
abstract = "Various strategies have been developed for the removal of synthetic dyes from the
wastewater of the textile industry due to their stability as well as their negative effect on the
environment. Enzymatic wastewater treatments as environmentally safe processes have many
benefits. An enzyme suitable for use in the treatment of wastewater from the textile industry
is horseradish peroxidase (HRP) because it can degrade different types of dyes. Immobilized
enzymes have many advantages over free enzymes in solution such as higher stability and the
opportunity to be removed easily from the reaction mixtures. Consequently, immobilized
enzymes can be used more than once. Many different materials are used as a carrier for
enzyme immobilization, so if residual material is used as a carrier, waste valorization will be
achieved. In this study immobilized commercial HRP was used for decolorization of
anthraquinone dye Acid green 40 (AG 40). Waste material – potato peels were used as a
carrier for the immobilization of HRP. Potato peels were oxidized with sodium periodate to
introduce aldehyde groups that can react with the primary amino groups of the enzyme to
form Schiff bases, resulting in covalently immobilized HRP. The success of the oxidation
was verified by the 2,4-dinitrophenylhydrazine (DNPH) test, and subsequently, the oxidized
potato peels were characterized by SEM and FTIR. After oxidation, the influence of the mass
of the immobilization carrier on enzyme activity and immobilization efficiency was
investigated. The optimal conditions of pH, hydrogen peroxide concentration, concentration
of AG 40, and enzyme activity were determined for decolorization of AG 40 with
immobilized HRP. Adsorption of AG 40 on the carrier was also determined. In addition, the
operational stability of the enzyme was determined. The results obtained show that oxidized
potato peels are a promising material that can be used as a carrier for the immobilization of
HRP.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023",
title = "New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye",
pages = "37",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7034"
}

Nedeljkov, T., Svetozarević Arsović, M., Šekuljica, N.,& Mijin, D.. (2023). New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023
Belgrade : University, Faculty of Technology and Metallurgy., 37.
https://hdl.handle.net/21.15107/rcub_technorep_7034

Nedeljkov T, Svetozarević Arsović M, Šekuljica N, Mijin D. New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023. 2023;:37.
https://hdl.handle.net/21.15107/rcub_technorep_7034 .

Nedeljkov, Tanja, Svetozarević Arsović, Milica, Šekuljica, Nataša, Mijin, Dušan, "New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023 (2023):37,
https://hdl.handle.net/21.15107/rcub_technorep_7034 .