TY  - CONF
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Meng, Huan
AU  - Li, Zhen
AU  - Grad, Sibylle
AU  - Panić, Vesna V.
AU  - Kalagasidis Krušić, Melina T.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7391
AB  - INTRODUCTION: Inflammation  process  in  human  body  can  lead  to  many serious inflammation-related  diseases. Hence, there are urge to find better solution for the treatment of the inflammation processes. Solution can be found in application  ofpoly(methacrylicacid)  hydrogels  which  have  potential  for  targeted  delivery  and  controlled  release  of drugs. These pH-sensitive hydrogelscan swell at the pH values between 5 and 8,and release drug in the process. So,taking into account that pH value at the inflammation site is around 6, these hydrogels are materials of choice. It  is  very  important thatthe system  for  controlled  releasebe  prepared  through mild  and  non-toxic  conditions  in order to preserve bioactivity of the drug andkeep good impact on environment. Enzymes are good candidates for eco-friendly preparation of hydrogels, because these greensubstancescan initiate polymerisation of various monomers. 
EXPERIMENTAL (or Materials and Methods):In this study, hydrogels based on poly(methacrylic acid) were prepared through  eco-friendly  method  by  using enzyme/hydrogen  peroxide(HP)/ascorbic  acid(AA)as  initiator.  Two  groupsof the samples were prepared: in the first group peroxidase isolated from potato peel waste (with activity of 0.8 IU) was used in the initiation system, whereas in the second group peroxidase isolated from soya bean coats (with activity of 0.8  IU) was  employed  in  the  initiation  system.  The  amounts  of HPand AAin  both  series  were  40  μL  and  10  mg, respectively.Anti-inflammatory drugs,dexamethasone(5 mg/mL)and diclofenac(4.5 mg/mL)were encapsulated in the first and the second group of the PMAA samples, respectively. Anti-inflammatory  effect of the PMAA hydrogels  with encapsulated drugs were tested on the Bovine Chondrocytes cells.
RESULTS  AND  DISCUSSION:Results  showed  that  the  level  of  pro-inflammatory  mediators  NO  and  IL-8  decreased. The PCR analysis showed that the proinflammatory TNα, IL-6 genesexpression level decreased, the matrix catabolism MMP1and MMP3 genesexpression level decreased and the fibrotic COL2 and ACAN genes expression level increased.
CONCLUSIONS:The studyshowed that the PMAA hydrogels have anti-inflammatory effect and have potential for treatment of the inflammation processes.
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method
IS  - 1S
SP  - 28
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7391
ER  - 

@conference{
author = "Marković, Maja D. and Svetozarević, Milica M. and Meng, Huan and Li, Zhen and Grad, Sibylle and Panić, Vesna V. and Kalagasidis Krušić, Melina T.",
year = "2024",
abstract = "INTRODUCTION: Inflammation  process  in  human  body  can  lead  to  many serious inflammation-related  diseases. Hence, there are urge to find better solution for the treatment of the inflammation processes. Solution can be found in application  ofpoly(methacrylicacid)  hydrogels  which  have  potential  for  targeted  delivery  and  controlled  release  of drugs. These pH-sensitive hydrogelscan swell at the pH values between 5 and 8,and release drug in the process. So,taking into account that pH value at the inflammation site is around 6, these hydrogels are materials of choice. It  is  very  important thatthe system  for  controlled  releasebe  prepared  through mild  and  non-toxic  conditions  in order to preserve bioactivity of the drug andkeep good impact on environment. Enzymes are good candidates for eco-friendly preparation of hydrogels, because these greensubstancescan initiate polymerisation of various monomers. 
EXPERIMENTAL (or Materials and Methods):In this study, hydrogels based on poly(methacrylic acid) were prepared through  eco-friendly  method  by  using enzyme/hydrogen  peroxide(HP)/ascorbic  acid(AA)as  initiator.  Two  groupsof the samples were prepared: in the first group peroxidase isolated from potato peel waste (with activity of 0.8 IU) was used in the initiation system, whereas in the second group peroxidase isolated from soya bean coats (with activity of 0.8  IU) was  employed  in  the  initiation  system.  The  amounts  of HPand AAin  both  series  were  40  μL  and  10  mg, respectively.Anti-inflammatory drugs,dexamethasone(5 mg/mL)and diclofenac(4.5 mg/mL)were encapsulated in the first and the second group of the PMAA samples, respectively. Anti-inflammatory  effect of the PMAA hydrogels  with encapsulated drugs were tested on the Bovine Chondrocytes cells.
RESULTS  AND  DISCUSSION:Results  showed  that  the  level  of  pro-inflammatory  mediators  NO  and  IL-8  decreased. The PCR analysis showed that the proinflammatory TNα, IL-6 genesexpression level decreased, the matrix catabolism MMP1and MMP3 genesexpression level decreased and the fibrotic COL2 and ACAN genes expression level increased.
CONCLUSIONS:The studyshowed that the PMAA hydrogels have anti-inflammatory effect and have potential for treatment of the inflammation processes.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method",
number = "1S",
pages = "28",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7391"
}

Marković, M. D., Svetozarević, M. M., Meng, H., Li, Z., Grad, S., Panić, V. V.,& Kalagasidis Krušić, M. T.. (2024). Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 28.
https://hdl.handle.net/21.15107/rcub_technorep_7391

Marković MD, Svetozarević MM, Meng H, Li Z, Grad S, Panić VV, Kalagasidis Krušić MT. Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):28.
https://hdl.handle.net/21.15107/rcub_technorep_7391 .

Marković, Maja D., Svetozarević, Milica M., Meng, Huan, Li, Zhen, Grad, Sibylle, Panić, Vesna V., Kalagasidis Krušić, Melina T., "Biocharacterization of hydrogels based on poly(methacrylic acid) prepared by eco-friendly method" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):28,
https://hdl.handle.net/21.15107/rcub_technorep_7391 .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Panić, Vesna V.
AU  - Savić, Sanja I.
AU  - Mašulović, Aleksandra D.
AU  - Spasojević, Pavle M.
AU  - Pjanović, Rada V.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5860
AB  - This study reports successful free radical synthesis of pH-sensitive hydrogels based on poly(methacrylic acid) (PMAA) by using new green initiation system based on vitamin C and hydrogen peroxide (VC/H2O2). The application of proposed initiation system provides many advantages, above all cost effective and eco-friendly synthesis which can be carried out under ambient conditions. The obtained PMAA hydrogels are analyzed by various technics: Differential Scanning Calorimetry, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy and by using single compression tests. In line with the intended application, PMAA hydrogels are further investigated in terms of their potential to be used for encapsulation and controlled release of active substances such as antioxidant phenolic compounds. To keep it green, the total phenolic compounds (TPC) were obtained from orange peels waste by applying ultrasonic-assisted extraction and deep eutectic solvent (DES) based on glycerol:urea:water. The TPC were successfully encapsulated into the PMAA hydrogels which were previously estimated to have the optimal mechanical and swelling properties with respect to the final application. The swelling behavior of the PMAA hydrogels and controlled release of the TPC were tested as a function of the various synthesis parameters in several media with different pH values. It was shown that TPC can be released in control manner in medium which simulates the environment in human intestines, finally resulting in enhanced bioavailability of TPC, reduced side effects and improved therapeutic effects.
PB  - Elsevier B.V.
T2  - Chemical Engineering Journal
T1  - Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds
SP  - 141580
VL  - 459
DO  - 10.1016/j.cej.2023.141580
ER  - 

@article{
author = "Marković, Maja D. and Svetozarević, Milica M. and Panić, Vesna V. and Savić, Sanja I. and Mašulović, Aleksandra D. and Spasojević, Pavle M. and Pjanović, Rada V.",
year = "2023",
abstract = "This study reports successful free radical synthesis of pH-sensitive hydrogels based on poly(methacrylic acid) (PMAA) by using new green initiation system based on vitamin C and hydrogen peroxide (VC/H2O2). The application of proposed initiation system provides many advantages, above all cost effective and eco-friendly synthesis which can be carried out under ambient conditions. The obtained PMAA hydrogels are analyzed by various technics: Differential Scanning Calorimetry, Fourier Transform Infrared spectroscopy, Scanning Electron Microscopy and by using single compression tests. In line with the intended application, PMAA hydrogels are further investigated in terms of their potential to be used for encapsulation and controlled release of active substances such as antioxidant phenolic compounds. To keep it green, the total phenolic compounds (TPC) were obtained from orange peels waste by applying ultrasonic-assisted extraction and deep eutectic solvent (DES) based on glycerol:urea:water. The TPC were successfully encapsulated into the PMAA hydrogels which were previously estimated to have the optimal mechanical and swelling properties with respect to the final application. The swelling behavior of the PMAA hydrogels and controlled release of the TPC were tested as a function of the various synthesis parameters in several media with different pH values. It was shown that TPC can be released in control manner in medium which simulates the environment in human intestines, finally resulting in enhanced bioavailability of TPC, reduced side effects and improved therapeutic effects.",
publisher = "Elsevier B.V.",
journal = "Chemical Engineering Journal",
title = "Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds",
pages = "141580",
volume = "459",
doi = "10.1016/j.cej.2023.141580"
}

Marković, M. D., Svetozarević, M. M., Panić, V. V., Savić, S. I., Mašulović, A. D., Spasojević, P. M.,& Pjanović, R. V.. (2023). Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds. in Chemical Engineering Journal
Elsevier B.V.., 459, 141580.
https://doi.org/10.1016/j.cej.2023.141580

Marković MD, Svetozarević MM, Panić VV, Savić SI, Mašulović AD, Spasojević PM, Pjanović RV. Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds. in Chemical Engineering Journal. 2023;459:141580.
doi:10.1016/j.cej.2023.141580 .

Marković, Maja D., Svetozarević, Milica M., Panić, Vesna V., Savić, Sanja I., Mašulović, Aleksandra D., Spasojević, Pavle M., Pjanović, Rada V., "Novel eco-friendly initiation system based on vitamin C for energy efficient synthesis of PMAA hydrogel used for delivery of phenolic compounds" in Chemical Engineering Journal, 459 (2023):141580,
https://doi.org/10.1016/j.cej.2023.141580 . .

TY  - CONF
AU  - Marković, Maja D.
AU  - Svetozarević, Milica M.
AU  - Ugrinović, Vukašin Đ.
AU  - Pjanović, Rada V.
AU  - Obradović, Bojana M.
AU  - Kalagasidis Krušić, Melina T.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6620
AB  - Everyday struggle of humanity with novel diseases and present once, urge researchers to find novel and improve existing therapies to enhance their efficiency and safety. One of the promising approaches to overcome these challenges is controlled release of drugs. Biomaterials based on poly(methacrylic acid) (PMAA) are excellent drug delivery systems because they can control release rate and released amount of drug. Also, due to their pH sensitivity the PMAA hydrogels can release drug at the site of action. Namely, these nontoxic and biocompatible hydrogels swell in the environment with pH value higher than pKa of PMAA (4.6) and release encapsulated drug during the process. In present study, PMAA hydrogels are synthetized under ambient conditions by simple, cost effective and eco-friendly synthesis. Novel initiation system based on hydrogen peroxide, potato peel peroxidase and vitamin C (VC) is used for the first time for free radical polymerization of PMAA hydrogel. In accordance with the principles of circular economy, peroxidase was isolated from potato peel waste by water extraction for 12 h at 4 ℃. Four PMAA hydrogels were prepared by using potato peel peroxidase with various enzyme activity (0.4; 0.8; 1.2 and 1.8 IU), whereas the amounts of H2O2 (30 mL) and VC (10 mg) have been kept constant. The composition of the PMAA hydrogels was confirmed by FTIR analysis, whereas their porous structure was revealed by SEM. The swelling of the PMAA hydrogels was monitored in two media: 0.1M HCl (as simulation of human stomach) and phosphate buffer with pH 7.4 (as simulation of human intestines). In order to encapsulate anti-inflammatory drug – dexamethasone into the PMAA hydrogels, the hydrogels were immersed into the dexamethasone aqueous solution (5 mg/ml) and left to swell to the equilibrium, after which they were dried at room temperature. Dexamethasone release from the PMAA hydrogels was monitored in the same environments as was the PMAA swelling. The results showed that around seven times higher amount of dexamethasone was released in the medium with pH 7.4 than in the medium with pH 1. Present study has showed that the PMAA hydrogels, prepared through green and eco-friendly method, have huge potential for encapsulation and controlled release of anti-inflammatory drugs and therefore, for the treatment of rheumatoid arthritis.
PB  - AO Foundation
C3  - ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical
T1  - Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs
SP  - 572
VL  - 3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6620
ER  - 

@conference{
author = "Marković, Maja D. and Svetozarević, Milica M. and Ugrinović, Vukašin Đ. and Pjanović, Rada V. and Obradović, Bojana M. and Kalagasidis Krušić, Melina T.",
year = "2023",
abstract = "Everyday struggle of humanity with novel diseases and present once, urge researchers to find novel and improve existing therapies to enhance their efficiency and safety. One of the promising approaches to overcome these challenges is controlled release of drugs. Biomaterials based on poly(methacrylic acid) (PMAA) are excellent drug delivery systems because they can control release rate and released amount of drug. Also, due to their pH sensitivity the PMAA hydrogels can release drug at the site of action. Namely, these nontoxic and biocompatible hydrogels swell in the environment with pH value higher than pKa of PMAA (4.6) and release encapsulated drug during the process. In present study, PMAA hydrogels are synthetized under ambient conditions by simple, cost effective and eco-friendly synthesis. Novel initiation system based on hydrogen peroxide, potato peel peroxidase and vitamin C (VC) is used for the first time for free radical polymerization of PMAA hydrogel. In accordance with the principles of circular economy, peroxidase was isolated from potato peel waste by water extraction for 12 h at 4 ℃. Four PMAA hydrogels were prepared by using potato peel peroxidase with various enzyme activity (0.4; 0.8; 1.2 and 1.8 IU), whereas the amounts of H2O2 (30 mL) and VC (10 mg) have been kept constant. The composition of the PMAA hydrogels was confirmed by FTIR analysis, whereas their porous structure was revealed by SEM. The swelling of the PMAA hydrogels was monitored in two media: 0.1M HCl (as simulation of human stomach) and phosphate buffer with pH 7.4 (as simulation of human intestines). In order to encapsulate anti-inflammatory drug – dexamethasone into the PMAA hydrogels, the hydrogels were immersed into the dexamethasone aqueous solution (5 mg/ml) and left to swell to the equilibrium, after which they were dried at room temperature. Dexamethasone release from the PMAA hydrogels was monitored in the same environments as was the PMAA swelling. The results showed that around seven times higher amount of dexamethasone was released in the medium with pH 7.4 than in the medium with pH 1. Present study has showed that the PMAA hydrogels, prepared through green and eco-friendly method, have huge potential for encapsulation and controlled release of anti-inflammatory drugs and therefore, for the treatment of rheumatoid arthritis.",
publisher = "AO Foundation",
journal = "ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical",
title = "Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs",
pages = "572",
volume = "3",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6620"
}

Marković, M. D., Svetozarević, M. M., Ugrinović, V. Đ., Pjanović, R. V., Obradović, B. M.,& Kalagasidis Krušić, M. T.. (2023). Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs. in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical
AO Foundation., 3, 572.
https://hdl.handle.net/21.15107/rcub_technorep_6620

Marković MD, Svetozarević MM, Ugrinović VĐ, Pjanović RV, Obradović BM, Kalagasidis Krušić MT. Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs. in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical. 2023;3:572.
https://hdl.handle.net/21.15107/rcub_technorep_6620 .

Marković, Maja D., Svetozarević, Milica M., Ugrinović, Vukašin Đ., Pjanović, Rada V., Obradović, Bojana M., Kalagasidis Krušić, Melina T., "Hydrogels based on poly(methacrylic acid) for controlled release of anti-inflammatory drugs" in ESB2023: 33rd Annual Conference of the European Society for Biomaterials, ARI Abstracts Periodical, 3 (2023):572,
https://hdl.handle.net/21.15107/rcub_technorep_6620 .

TY  - CONF
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Marković, Maja
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Mijin, Dušan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6668
AB  - Agro-industrial waste has gained special attention as an abundatly available natural carbon source, which can be used for value-added products such as generating power, biofuel, biogas and biofertilizers production. Further application of agro-industrial waste may be as a substrate for solid state fermentation for production of antibiotics, enzymes, and phytochemicals. Isolation of enzymes from waste material as crude extracts contributes to the concept of sustainability and lowers their cost significantly. The enzyme of interest in this study was peroxidase isolated from soybean hull. Furthermore, the crude peroxidase from soybean hull was crosslinked by oxidized pectin onto the inner walls of PTFE microtubular reactor. The influence of the crosslinker concentration on the enzyme activity and crosslinking efficiency was evaluated, together with the effect of the reagents flow rate on the enzyme activity and crosslinking efficiency. Two different microtubular diameters were examined for the crude peroxidase crosslinking: 0.5 and 0.8 mm. After the immobilization, the possibility of anthraquinone dye removal by crosslinked peroxidase in the microtubular reactor was examined. The reusability of the immobilized enzyme was also evaluated in this study.
AB  - Poljoprivredni otpad predstavlja lako dostupan prirodni izvor ugljenika, koji se može koristiti za dobijanje proizvoda sa dodatom vrednošću. Poljoprivredni otpad se može koristiti kao sirovina za proizvodnju električne energije, biogorivo, biogas i proizvodnju biođubriva. Dalja primena poljoprivrednog otpada može biti kao supstrat za fermentaciju u čvrstom stanju (eng. solid state fermentation), za proizvodnju antibiotika, enzima i fitohemikalija. Izolovanje enzima iz otpadnog materijala kao sirovi ekstrakt doprinosi konceptu održivosti i značajno smanjuje cenu enzima. Enzim od interesa u ovom radu je peroksidaza izolovana iz sojinih ljuspica. Sirova peroksidaza iz sojinih ljuspica umrežena je oksidovanim pektinom na unutrašnje zidove PTFE cevnog mikroreaktor, i ispitivan je uticaj koncentracije umreživača na aktivnost enzima i efikasnost umrežavanja, kao i uticaj protoka reagensa na aktivnost enzima i efikasnost umrežavanja. Nakon toga, vršeno je ispitivanje uticaja prečnika cevnog mikroreaktora na efikasnost umrežavanja i aktivnost peroksidaze: 0,5 i 0,8 mm. Nakon imobilizacije ispitana je mogućnost uklanjanja antrahinonske boje umreženom peroksidazom u cevnom mikroreaktoru. U ovom radu je takođe je ispitivana mogućnost ponovne upotrebe imobilisanog enzima.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac
T1  - Flow in the tube: crosslinking of peroxidase from agricultural waste for dye removal in microtubular reactor
T1  - Kontinualni sistem za obezbojavanje otpadnih voda. Primena umrežene peroksidaze iz poljoprivrednog otpada u uklanjanju boje
EP  - 112
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6668
ER  - 

@conference{
author = "Svetozarević, Milica and Šekuljica, Nataša and Marković, Maja and Dajić, Ana and Mihajlović, Marina and Mijin, Dušan",
year = "2023",
abstract = "Agro-industrial waste has gained special attention as an abundatly available natural carbon source, which can be used for value-added products such as generating power, biofuel, biogas and biofertilizers production. Further application of agro-industrial waste may be as a substrate for solid state fermentation for production of antibiotics, enzymes, and phytochemicals. Isolation of enzymes from waste material as crude extracts contributes to the concept of sustainability and lowers their cost significantly. The enzyme of interest in this study was peroxidase isolated from soybean hull. Furthermore, the crude peroxidase from soybean hull was crosslinked by oxidized pectin onto the inner walls of PTFE microtubular reactor. The influence of the crosslinker concentration on the enzyme activity and crosslinking efficiency was evaluated, together with the effect of the reagents flow rate on the enzyme activity and crosslinking efficiency. Two different microtubular diameters were examined for the crude peroxidase crosslinking: 0.5 and 0.8 mm. After the immobilization, the possibility of anthraquinone dye removal by crosslinked peroxidase in the microtubular reactor was examined. The reusability of the immobilized enzyme was also evaluated in this study., Poljoprivredni otpad predstavlja lako dostupan prirodni izvor ugljenika, koji se može koristiti za dobijanje proizvoda sa dodatom vrednošću. Poljoprivredni otpad se može koristiti kao sirovina za proizvodnju električne energije, biogorivo, biogas i proizvodnju biođubriva. Dalja primena poljoprivrednog otpada može biti kao supstrat za fermentaciju u čvrstom stanju (eng. solid state fermentation), za proizvodnju antibiotika, enzima i fitohemikalija. Izolovanje enzima iz otpadnog materijala kao sirovi ekstrakt doprinosi konceptu održivosti i značajno smanjuje cenu enzima. Enzim od interesa u ovom radu je peroksidaza izolovana iz sojinih ljuspica. Sirova peroksidaza iz sojinih ljuspica umrežena je oksidovanim pektinom na unutrašnje zidove PTFE cevnog mikroreaktor, i ispitivan je uticaj koncentracije umreživača na aktivnost enzima i efikasnost umrežavanja, kao i uticaj protoka reagensa na aktivnost enzima i efikasnost umrežavanja. Nakon toga, vršeno je ispitivanje uticaja prečnika cevnog mikroreaktora na efikasnost umrežavanja i aktivnost peroksidaze: 0,5 i 0,8 mm. Nakon imobilizacije ispitana je mogućnost uklanjanja antrahinonske boje umreženom peroksidazom u cevnom mikroreaktoru. U ovom radu je takođe je ispitivana mogućnost ponovne upotrebe imobilisanog enzima.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac",
title = "Flow in the tube: crosslinking of peroxidase from agricultural waste for dye removal in microtubular reactor, Kontinualni sistem za obezbojavanje otpadnih voda. Primena umrežene peroksidaze iz poljoprivrednog otpada u uklanjanju boje",
pages = "112-107",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6668"
}

Svetozarević, M., Šekuljica, N., Marković, M., Dajić, A., Mihajlović, M.,& Mijin, D.. (2023). Flow in the tube: crosslinking of peroxidase from agricultural waste for dye removal in microtubular reactor. in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 107-112.
https://hdl.handle.net/21.15107/rcub_technorep_6668

Svetozarević M, Šekuljica N, Marković M, Dajić A, Mihajlović M, Mijin D. Flow in the tube: crosslinking of peroxidase from agricultural waste for dye removal in microtubular reactor. in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac. 2023;:107-112.
https://hdl.handle.net/21.15107/rcub_technorep_6668 .

Svetozarević, Milica, Šekuljica, Nataša, Marković, Maja, Dajić, Ana, Mihajlović, Marina, Mijin, Dušan, "Flow in the tube: crosslinking of peroxidase from agricultural waste for dye removal in microtubular reactor" in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac (2023):107-112,
https://hdl.handle.net/21.15107/rcub_technorep_6668 .

TY  - CONF
AU  - Svetozarević Arsović, Milica
AU  - Šekuljica, Nataša
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Marković, Maja
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6741
AB  - Wastewater from textile industry contains considerable amount of dissolved dye that can trigger environmental issues if is not treated properly. Numerous methods have been developed to degrade recalcitrant pollutants safely and utterly. Among them, enzymatic treatment of wastewater is gaining attention due to the enzyme’s specificity, easier manipulation, and generation of less toxic by-products. Still, the cost of enzymatic systems is the main limitation keeping the biocatalysts at lab-scale. Alternative solution for reducing the cost of enzymatic reaction systems is the use of microfluidic systems, which contribute to better mixing, process intensification and cleaner production. In this study, implementation of horseradish peroxidase for removal of the textile dye Acid Violet 109 is performed in a microfluidic reactor. The microreactor consists of three plunger pump units, two mixers and PTFE tube. The process parameters: residence time, dye, hydrogen peroxide, enzyme activity, the reactors’ diameter and length were optimized. Under the optimal process conditions: 30 mg/L dye concentration, 0.8 U/mL horseradish peroxidase activity, 0.1 mM hydrogen peroxide, 0.25 mm reactor's diameter, 97,3 % removal was achieved at residence time of 6 min. The results from this study show that enzymatic microfluidic reactors are a convenient technology for dye removal.
PB  - EDP Sciences
C3  - E3S Web of Conferences
T1  - Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor
SP  - 10003
VL  - 436
DO  - 10.1051/e3sconf/202343610003
ER  - 

@conference{
author = "Svetozarević Arsović, Milica and Šekuljica, Nataša and Dajić, Ana and Mihajlović, Marina and Marković, Maja and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2023",
abstract = "Wastewater from textile industry contains considerable amount of dissolved dye that can trigger environmental issues if is not treated properly. Numerous methods have been developed to degrade recalcitrant pollutants safely and utterly. Among them, enzymatic treatment of wastewater is gaining attention due to the enzyme’s specificity, easier manipulation, and generation of less toxic by-products. Still, the cost of enzymatic systems is the main limitation keeping the biocatalysts at lab-scale. Alternative solution for reducing the cost of enzymatic reaction systems is the use of microfluidic systems, which contribute to better mixing, process intensification and cleaner production. In this study, implementation of horseradish peroxidase for removal of the textile dye Acid Violet 109 is performed in a microfluidic reactor. The microreactor consists of three plunger pump units, two mixers and PTFE tube. The process parameters: residence time, dye, hydrogen peroxide, enzyme activity, the reactors’ diameter and length were optimized. Under the optimal process conditions: 30 mg/L dye concentration, 0.8 U/mL horseradish peroxidase activity, 0.1 mM hydrogen peroxide, 0.25 mm reactor's diameter, 97,3 % removal was achieved at residence time of 6 min. The results from this study show that enzymatic microfluidic reactors are a convenient technology for dye removal.",
publisher = "EDP Sciences",
journal = "E3S Web of Conferences",
title = "Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor",
pages = "10003",
volume = "436",
doi = "10.1051/e3sconf/202343610003"
}

Svetozarević Arsović, M., Šekuljica, N., Dajić, A., Mihajlović, M., Marković, M., Knežević-Jugović, Z.,& Mijin, D.. (2023). Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor. in E3S Web of Conferences
EDP Sciences., 436, 10003.
https://doi.org/10.1051/e3sconf/202343610003

Svetozarević Arsović M, Šekuljica N, Dajić A, Mihajlović M, Marković M, Knežević-Jugović Z, Mijin D. Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor. in E3S Web of Conferences. 2023;436:10003.
doi:10.1051/e3sconf/202343610003 .

Svetozarević Arsović, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Marković, Maja, Knežević-Jugović, Zorica, Mijin, Dušan, "Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor" in E3S Web of Conferences, 436 (2023):10003,
https://doi.org/10.1051/e3sconf/202343610003 . .

TY  - PAT
AU  - Mijin, Dušan
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Knežević-Jugović, Zorica
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Jovanović, Mića
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6660
AB  - Predmetni pronalazak se odnosi na postupak biodegradacije antrahinonske boje Acid Violet 109 peroksidazom, izolovanom iz poljoprivrednog otpada u kontinualnom mikroreaktorskom sistemu. Postupak se sastoji od ekstrakcije enzima i biodegradacije boje u mikroreaktoru. Koncentracija boje treba da bude do 450 mg/L, enzimski ekstrakt aktivnosti 1 U/mL i vodonik-peroksid koncentracije do 1 mM, da bi se postigao najveći stepen biodegradacije. Na ovaj način dobija se visoko efikasan postupak biodegradacije antrahinonskih boja (~97 %) koji je održiv, ekološki i ekonomski opravdan.
AB  - The present invention relates to a method for anthraquinone dye Acid Violet 109 biodegradation using peroxidase, isolated from agro-industrial waste in continuous microreactor system. The method consists of enzyme extraction and dye biodegradation in microreactor. Dye concentration should be up to 450 mg/L, enzyme extract with the activity of 1 U/mL and hydrogen peroxide concentration up to 1 mM, in order to achieve the highest level of biodegradation. In this way highly efficient method for anthraquinone dyes biodegradation is obtained (~97 %) which is sustainable, ecologically and economically justified.
PB  - Zavod za intelektualnu svojinu Republike Srbije
T2  - Glasnik intelektualne svojine
T1  - Novi postupak za biodegradaciju antrahinonskih boja u kontinualnom mikroreaktorskom sistemu peroksidazom izolovanom iz poljoprivrednog otpada
T1  - New method for anthraquinone dyes' biodegradation in continuous microreactor using peroxidase from agro-industrial waste
SP  - 22
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6660
ER  - 

@misc{
author = "Mijin, Dušan and Svetozarević, Milica and Šekuljica, Nataša and Knežević-Jugović, Zorica and Dajić, Ana and Mihajlović, Marina and Jovanović, Mića",
year = "2023",
abstract = "Predmetni pronalazak se odnosi na postupak biodegradacije antrahinonske boje Acid Violet 109 peroksidazom, izolovanom iz poljoprivrednog otpada u kontinualnom mikroreaktorskom sistemu. Postupak se sastoji od ekstrakcije enzima i biodegradacije boje u mikroreaktoru. Koncentracija boje treba da bude do 450 mg/L, enzimski ekstrakt aktivnosti 1 U/mL i vodonik-peroksid koncentracije do 1 mM, da bi se postigao najveći stepen biodegradacije. Na ovaj način dobija se visoko efikasan postupak biodegradacije antrahinonskih boja (~97 %) koji je održiv, ekološki i ekonomski opravdan., The present invention relates to a method for anthraquinone dye Acid Violet 109 biodegradation using peroxidase, isolated from agro-industrial waste in continuous microreactor system. The method consists of enzyme extraction and dye biodegradation in microreactor. Dye concentration should be up to 450 mg/L, enzyme extract with the activity of 1 U/mL and hydrogen peroxide concentration up to 1 mM, in order to achieve the highest level of biodegradation. In this way highly efficient method for anthraquinone dyes biodegradation is obtained (~97 %) which is sustainable, ecologically and economically justified.",
publisher = "Zavod za intelektualnu svojinu Republike Srbije",
journal = "Glasnik intelektualne svojine",
title = "Novi postupak za biodegradaciju antrahinonskih boja u kontinualnom mikroreaktorskom sistemu peroksidazom izolovanom iz poljoprivrednog otpada, New method for anthraquinone dyes' biodegradation in continuous microreactor using peroxidase from agro-industrial waste",
pages = "22",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6660"
}

Mijin, D., Svetozarević, M., Šekuljica, N., Knežević-Jugović, Z., Dajić, A., Mihajlović, M.,& Jovanović, M.. (2023). Novi postupak za biodegradaciju antrahinonskih boja u kontinualnom mikroreaktorskom sistemu peroksidazom izolovanom iz poljoprivrednog otpada. in Glasnik intelektualne svojine
Zavod za intelektualnu svojinu Republike Srbije., 5, 22.
https://hdl.handle.net/21.15107/rcub_technorep_6660

Mijin D, Svetozarević M, Šekuljica N, Knežević-Jugović Z, Dajić A, Mihajlović M, Jovanović M. Novi postupak za biodegradaciju antrahinonskih boja u kontinualnom mikroreaktorskom sistemu peroksidazom izolovanom iz poljoprivrednog otpada. in Glasnik intelektualne svojine. 2023;5:22.
https://hdl.handle.net/21.15107/rcub_technorep_6660 .

Mijin, Dušan, Svetozarević, Milica, Šekuljica, Nataša, Knežević-Jugović, Zorica, Dajić, Ana, Mihajlović, Marina, Jovanović, Mića, "Novi postupak za biodegradaciju antrahinonskih boja u kontinualnom mikroreaktorskom sistemu peroksidazom izolovanom iz poljoprivrednog otpada" in Glasnik intelektualne svojine, 5 (2023):22,
https://hdl.handle.net/21.15107/rcub_technorep_6660 .

TY  - PAT
AU  - Svetozarević Arsović, Milica
AU  - Šekuljica, Nataša
AU  - Stupar, Stevan
AU  - Nedeljkov, Tanja
AU  - Ivanovska, Aleksandra
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6970
T2  - Zavod za intelektualnu svojinu republike Srbije
T1  - Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6970
ER  - 

@misc{
author = "Svetozarević Arsović, Milica and Šekuljica, Nataša and Stupar, Stevan and Nedeljkov, Tanja and Ivanovska, Aleksandra and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2023",
journal = "Zavod za intelektualnu svojinu republike Srbije",
title = "Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6970"
}

Svetozarević Arsović, M., Šekuljica, N., Stupar, S., Nedeljkov, T., Ivanovska, A., Knežević-Jugović, Z.,& Mijin, D.. (2023). Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira. in Zavod za intelektualnu svojinu republike Srbije.
https://hdl.handle.net/21.15107/rcub_technorep_6970

Svetozarević Arsović M, Šekuljica N, Stupar S, Nedeljkov T, Ivanovska A, Knežević-Jugović Z, Mijin D. Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira. in Zavod za intelektualnu svojinu republike Srbije. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6970 .

Svetozarević Arsović, Milica, Šekuljica, Nataša, Stupar, Stevan, Nedeljkov, Tanja, Ivanovska, Aleksandra, Knežević-Jugović, Zorica, Mijin, Dušan, "Postupak za imobilizaciju enzima na oksidovanim ljuskama krompira" in Zavod za intelektualnu svojinu republike Srbije (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6970 .

TY  - CONF
AU  - Nedeljkov, Tanja
AU  - Svetozarević Arsović, Milica
AU  - Šekuljica, Nataša
AU  - Mijin, Dušan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7034
AB  - Various strategies have been developed for the removal of synthetic dyes from the
wastewater of the textile industry due to their stability as well as their negative effect on the
environment. Enzymatic wastewater treatments as environmentally safe processes have many
benefits. An enzyme suitable for use in the treatment of wastewater from the textile industry
is horseradish peroxidase (HRP) because it can degrade different types of dyes. Immobilized
enzymes have many advantages over free enzymes in solution such as higher stability and the
opportunity to be removed easily from the reaction mixtures. Consequently, immobilized
enzymes can be used more than once. Many different materials are used as a carrier for
enzyme immobilization, so if residual material is used as a carrier, waste valorization will be
achieved. In this study immobilized commercial HRP was used for decolorization of
anthraquinone dye Acid green 40 (AG 40). Waste material – potato peels were used as a
carrier for the immobilization of HRP. Potato peels were oxidized with sodium periodate to
introduce aldehyde groups that can react with the primary amino groups of the enzyme to
form Schiff bases, resulting in covalently immobilized HRP. The success of the oxidation
was verified by the 2,4-dinitrophenylhydrazine (DNPH) test, and subsequently, the oxidized
potato peels were characterized by SEM and FTIR. After oxidation, the influence of the mass
of the immobilization carrier on enzyme activity and immobilization efficiency was
investigated. The optimal conditions of pH, hydrogen peroxide concentration, concentration
of AG 40, and enzyme activity were determined for decolorization of AG 40 with
immobilized HRP. Adsorption of AG 40 on the carrier was also determined. In addition, the
operational stability of the enzyme was determined. The results obtained show that oxidized
potato peels are a promising material that can be used as a carrier for the immobilization of
HRP.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023
T1  - New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7034
ER  - 

@conference{
author = "Nedeljkov, Tanja and Svetozarević Arsović, Milica and Šekuljica, Nataša and Mijin, Dušan",
year = "2023",
abstract = "Various strategies have been developed for the removal of synthetic dyes from the
wastewater of the textile industry due to their stability as well as their negative effect on the
environment. Enzymatic wastewater treatments as environmentally safe processes have many
benefits. An enzyme suitable for use in the treatment of wastewater from the textile industry
is horseradish peroxidase (HRP) because it can degrade different types of dyes. Immobilized
enzymes have many advantages over free enzymes in solution such as higher stability and the
opportunity to be removed easily from the reaction mixtures. Consequently, immobilized
enzymes can be used more than once. Many different materials are used as a carrier for
enzyme immobilization, so if residual material is used as a carrier, waste valorization will be
achieved. In this study immobilized commercial HRP was used for decolorization of
anthraquinone dye Acid green 40 (AG 40). Waste material – potato peels were used as a
carrier for the immobilization of HRP. Potato peels were oxidized with sodium periodate to
introduce aldehyde groups that can react with the primary amino groups of the enzyme to
form Schiff bases, resulting in covalently immobilized HRP. The success of the oxidation
was verified by the 2,4-dinitrophenylhydrazine (DNPH) test, and subsequently, the oxidized
potato peels were characterized by SEM and FTIR. After oxidation, the influence of the mass
of the immobilization carrier on enzyme activity and immobilization efficiency was
investigated. The optimal conditions of pH, hydrogen peroxide concentration, concentration
of AG 40, and enzyme activity were determined for decolorization of AG 40 with
immobilized HRP. Adsorption of AG 40 on the carrier was also determined. In addition, the
operational stability of the enzyme was determined. The results obtained show that oxidized
potato peels are a promising material that can be used as a carrier for the immobilization of
HRP.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023",
title = "New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye",
pages = "37",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7034"
}

Nedeljkov, T., Svetozarević Arsović, M., Šekuljica, N.,& Mijin, D.. (2023). New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023
Belgrade : University, Faculty of Technology and Metallurgy., 37.
https://hdl.handle.net/21.15107/rcub_technorep_7034

Nedeljkov T, Svetozarević Arsović M, Šekuljica N, Mijin D. New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye. in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023. 2023;:37.
https://hdl.handle.net/21.15107/rcub_technorep_7034 .

Nedeljkov, Tanja, Svetozarević Arsović, Milica, Šekuljica, Nataša, Mijin, Dušan, "New life of waste material: immobilized horseradish peroxidase for degradation of antraquinone dye" in Book of Abstracts / International Conference Biochemical Engineering and Biotechnology for Young Scientists, Belgrade, 2023 (2023):37,
https://hdl.handle.net/21.15107/rcub_technorep_7034 .

TY  - CONF
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Svetozarević Arsović, Milica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6954
AB  - Climate change projections indicate that Republic of Serbia and the Western Balkans will
face a high probability of continuing temperature increases, climatic extremes are projected to become
more common, including a significant increase in the number of extreme heat events. Heavier
precipitation events are expected in the winter months, whilst summers are projected to become even
drier. This paper aims to analyse effect of precipitation changes on landfill gas generation on the
Vinča landfill, the biggest landfill in Serbia. Quantities of generated landfill gas and methane have
been estimated by using LandGem model. Site specific methane generation rate has been calculated
according to GMI methodology for Central Eastern Europe. Sensitivity analyses is performed to
determine influence of a precipitation regimes on the methane generation rate on the site. Any increase
in precipitation (in range 10-30%) may increase landfill gas production for 4.3%. The paper shows
the difference in gas emissions, with the same composition of waste, and different precipitation
regimes. Changes in precipitation regimes due to climate change can affect the dynamics of landfill
gas emissions. This information is significant for the possible re-circulation of leachate from the
landfill which could result in an increased production of landfill gas.
PB  - EDP Sciences
C3  - E3S Web of Conferences
T1  - The effect of climate change on the potential for landfill gas generation at the Vinča landfill site
SP  - 02010
VL  - 436
DO  - 10.1051/e3sconf/202343602010
ER  - 

@conference{
author = "Dajić, Ana and Mihajlović, Marina and Svetozarević Arsović, Milica",
year = "2023",
abstract = "Climate change projections indicate that Republic of Serbia and the Western Balkans will
face a high probability of continuing temperature increases, climatic extremes are projected to become
more common, including a significant increase in the number of extreme heat events. Heavier
precipitation events are expected in the winter months, whilst summers are projected to become even
drier. This paper aims to analyse effect of precipitation changes on landfill gas generation on the
Vinča landfill, the biggest landfill in Serbia. Quantities of generated landfill gas and methane have
been estimated by using LandGem model. Site specific methane generation rate has been calculated
according to GMI methodology for Central Eastern Europe. Sensitivity analyses is performed to
determine influence of a precipitation regimes on the methane generation rate on the site. Any increase
in precipitation (in range 10-30%) may increase landfill gas production for 4.3%. The paper shows
the difference in gas emissions, with the same composition of waste, and different precipitation
regimes. Changes in precipitation regimes due to climate change can affect the dynamics of landfill
gas emissions. This information is significant for the possible re-circulation of leachate from the
landfill which could result in an increased production of landfill gas.",
publisher = "EDP Sciences",
journal = "E3S Web of Conferences",
title = "The effect of climate change on the potential for landfill gas generation at the Vinča landfill site",
pages = "02010",
volume = "436",
doi = "10.1051/e3sconf/202343602010"
}

Dajić, A., Mihajlović, M.,& Svetozarević Arsović, M.. (2023). The effect of climate change on the potential for landfill gas generation at the Vinča landfill site. in E3S Web of Conferences
EDP Sciences., 436, 02010.
https://doi.org/10.1051/e3sconf/202343602010

Dajić A, Mihajlović M, Svetozarević Arsović M. The effect of climate change on the potential for landfill gas generation at the Vinča landfill site. in E3S Web of Conferences. 2023;436:02010.
doi:10.1051/e3sconf/202343602010 .

Dajić, Ana, Mihajlović, Marina, Svetozarević Arsović, Milica, "The effect of climate change on the potential for landfill gas generation at the Vinča landfill site" in E3S Web of Conferences, 436 (2023):02010,
https://doi.org/10.1051/e3sconf/202343602010 . .

TY  - CONF
AU  - Mašulović, Aleksandra
AU  - Svetozarević, Milica
AU  - Ivanovska, Aleksandra
AU  - Lazić, Anita
AU  - Tadić, Julijana
AU  - Mijin, Dušan
AU  - Lađarević, Jelena
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6666
AB  - Wide commercial application of azo pyridone dyes rests on the cost-effectiveness of their preparation and almost innumerable possibilities for the synthesis by combining various diazo and coupling pyridone components that enable tuning of targeted properties. The great significance of these dyes in the last few decades is due to their outstanding properties, such as vivid colors and good color fastness properties. Azo pyridone dyes are also applicable in different coloration related processes such as inkjet printing and liquid crystal displays. On the other hand, they are recognized as valuable compounds in biological studies, as they possess antioxidant, antimicrobial and antiproliferative properties. In this work, three novel 6-hydroxy-4-methyl-1-pyridinium-2-pyridone based dyes bearing three bioactive heterocyclic rings in their structures are synthesized, wherein thiazole based anilines (2-aminothiazole, 2-aminobenzothiazole, 6-methoxy-2-aminobenzothiazole) are used as diazo components. The compounds are characterized by melting points, NMR and FTIR spectroscopy. The solvatochromic properties are investigated in solvents of different properties. Furthermore, the antioxidant capacities of the compounds are evaluated by ABTS (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) assay, while antimicrobial activity is determined by broth microdilution method against five bacterial strains (S. aureus, E. faecalis, B. subtilis, E. coli and K. pneumoniae) and one fungal strain (C. albicans).
AB  - Široka komercijalna upotreba piridonskih azo boja zasniva se na njihovoj relativno jeftinoj i jednostavnoj pripremi, kao i činjenici da se veliki broj kuplujućih i diazo komponenti mogu međusobno kombinovati kako bi se dobile boje odgovarajućih svojstava. Veliki značaj ovih boja poslednjih nekoliko decenija, ogleda se u njihovim istaknutim svojstvima kao što su živopisna obojenja i dobra postojanost. Ove boje, takođe, nalaze primenu u ink-džet štampi i LCD ekranima. Sa druge strane, ove boje predstavljaju značajna jedinjenja u biološkim studijama, jer poseduju antioksidativnu, antimikrobnu i antiproliferativnu aktivnost. U ovom radu, sintetisane su tri boje koje u svojoj strukturi obuhvataju tri bioakativna heterociklična jezgra, pri čemu je kao kuplujuća komponenta korišćen 6-hidroksi-4-metil-1-piridinijum-2-piridon, a kao diazo komponente anilini na bazi tiazola (2-aminotiazol, 2-aminobenzotiazol i 6-metoksi-2-aminobenzotiazol). Jedinjenja su okarakterisana temperaturom topljenja, NMR i FTIR spektroskopijama. Solvatohromna svojstva su ispitana u rastvaračima različitih svojstava. Antioksidativna aktivnost jedinjenja ispitana je ABTS (2,2'-azino-bis-3-etilbenzotiazolin-6-sulfonat) testom, dok je minimalna inhibitorna koncentracija ispitana primenom mikrodilucione metode u bujonu prema 5 bakterijskih sojeva (S. aureus, E. faecalis, B. subtilis, E. coli i K. pneumoniae), kao i prema oportunističkoj gljivici C. albicans.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac
T1  - Multipurpose azo pyridone dyes: synthesis, characterization, antioxidant and antimicrobial activity
T1  - Azo piridonske boje za višenamensku upotrebu: sinteza, karakterizacija, antioksidativna i antimikrobna aktivnost
EP  - 196
SP  - 191
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6666
ER  - 

@conference{
author = "Mašulović, Aleksandra and Svetozarević, Milica and Ivanovska, Aleksandra and Lazić, Anita and Tadić, Julijana and Mijin, Dušan and Lađarević, Jelena",
year = "2023",
abstract = "Wide commercial application of azo pyridone dyes rests on the cost-effectiveness of their preparation and almost innumerable possibilities for the synthesis by combining various diazo and coupling pyridone components that enable tuning of targeted properties. The great significance of these dyes in the last few decades is due to their outstanding properties, such as vivid colors and good color fastness properties. Azo pyridone dyes are also applicable in different coloration related processes such as inkjet printing and liquid crystal displays. On the other hand, they are recognized as valuable compounds in biological studies, as they possess antioxidant, antimicrobial and antiproliferative properties. In this work, three novel 6-hydroxy-4-methyl-1-pyridinium-2-pyridone based dyes bearing three bioactive heterocyclic rings in their structures are synthesized, wherein thiazole based anilines (2-aminothiazole, 2-aminobenzothiazole, 6-methoxy-2-aminobenzothiazole) are used as diazo components. The compounds are characterized by melting points, NMR and FTIR spectroscopy. The solvatochromic properties are investigated in solvents of different properties. Furthermore, the antioxidant capacities of the compounds are evaluated by ABTS (2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) assay, while antimicrobial activity is determined by broth microdilution method against five bacterial strains (S. aureus, E. faecalis, B. subtilis, E. coli and K. pneumoniae) and one fungal strain (C. albicans)., Široka komercijalna upotreba piridonskih azo boja zasniva se na njihovoj relativno jeftinoj i jednostavnoj pripremi, kao i činjenici da se veliki broj kuplujućih i diazo komponenti mogu međusobno kombinovati kako bi se dobile boje odgovarajućih svojstava. Veliki značaj ovih boja poslednjih nekoliko decenija, ogleda se u njihovim istaknutim svojstvima kao što su živopisna obojenja i dobra postojanost. Ove boje, takođe, nalaze primenu u ink-džet štampi i LCD ekranima. Sa druge strane, ove boje predstavljaju značajna jedinjenja u biološkim studijama, jer poseduju antioksidativnu, antimikrobnu i antiproliferativnu aktivnost. U ovom radu, sintetisane su tri boje koje u svojoj strukturi obuhvataju tri bioakativna heterociklična jezgra, pri čemu je kao kuplujuća komponenta korišćen 6-hidroksi-4-metil-1-piridinijum-2-piridon, a kao diazo komponente anilini na bazi tiazola (2-aminotiazol, 2-aminobenzotiazol i 6-metoksi-2-aminobenzotiazol). Jedinjenja su okarakterisana temperaturom topljenja, NMR i FTIR spektroskopijama. Solvatohromna svojstva su ispitana u rastvaračima različitih svojstava. Antioksidativna aktivnost jedinjenja ispitana je ABTS (2,2'-azino-bis-3-etilbenzotiazolin-6-sulfonat) testom, dok je minimalna inhibitorna koncentracija ispitana primenom mikrodilucione metode u bujonu prema 5 bakterijskih sojeva (S. aureus, E. faecalis, B. subtilis, E. coli i K. pneumoniae), kao i prema oportunističkoj gljivici C. albicans.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac",
title = "Multipurpose azo pyridone dyes: synthesis, characterization, antioxidant and antimicrobial activity, Azo piridonske boje za višenamensku upotrebu: sinteza, karakterizacija, antioksidativna i antimikrobna aktivnost",
pages = "196-191",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6666"
}

Mašulović, A., Svetozarević, M., Ivanovska, A., Lazić, A., Tadić, J., Mijin, D.,& Lađarević, J.. (2023). Multipurpose azo pyridone dyes: synthesis, characterization, antioxidant and antimicrobial activity. in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 191-196.
https://hdl.handle.net/21.15107/rcub_technorep_6666

Mašulović A, Svetozarević M, Ivanovska A, Lazić A, Tadić J, Mijin D, Lađarević J. Multipurpose azo pyridone dyes: synthesis, characterization, antioxidant and antimicrobial activity. in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac. 2023;:191-196.
https://hdl.handle.net/21.15107/rcub_technorep_6666 .

Mašulović, Aleksandra, Svetozarević, Milica, Ivanovska, Aleksandra, Lazić, Anita, Tadić, Julijana, Mijin, Dušan, Lađarević, Jelena, "Multipurpose azo pyridone dyes: synthesis, characterization, antioxidant and antimicrobial activity" in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac (2023):191-196,
https://hdl.handle.net/21.15107/rcub_technorep_6666 .

TY  - JOUR
AU  - Ivanovska, Aleksandra
AU  - Dojčinović, Biljana
AU  - Lađarević, Jelena
AU  - Pavun, Leposava
AU  - Mijin, Dušan
AU  - Kostić, Mirjana
AU  - Svetozarević, Milica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6223
AB  - This study is aimed at extending the soybean hulls' lifetime by their utilization as an adsorbent for metal ions (Cd2+ and Cu2+) and dyes (Reactive Yellow 39 (RY 39) and Acid Blue 225 (AB 225)). ATR-FTIR spectroscopy, FE-SEM microscopy, and zeta potential measurements were used for adsorbent characterization. The effect of the solution's pH, peroxidase extraction, adsorbent particle size, contact time, the pollutant's initial concentration, and temperature on the soybean hulls' adsorption potential was studied. Before peroxidase extraction, soybean hulls were capable of removing 72% Cd2+, 71% Cu2+ (at a pH of 5.00) or 81% RY 39, and 73% AB 225 (at a pH of 3.00). For further experiments, soybean hulls without peroxidase were used for several reasons: (1) due to their observed higher metal ion removal, (2) in order to reduce the waste disposal cost after the peroxidase (usually used for wastewater decolorization) extraction, and (3) since the soybean hulls without peroxidase possessed significantly lower secondary pollution than those with peroxidase. Cd2+ and Cu2+ removal was slightly increased when the smaller adsorbent fraction (710-1000 μm) was used, while the adsorbent particle size did not have an impact on dye removal. After 30 min of contact time, 92% and 88% of RY 39 and AB 225 were removed, respectively, while after the same contact time, 80% and 69% of Cd2+ and Cu2+ were removed, respectively. Adsorption of all tested pollutants follows a pseudo-second-order reaction through the fast adsorption, intraparticle diffusion, and final equilibrium stage. The maximal adsorption capacities determined by the Langmuir model were 21.10, 20.54, 16.54, and 17.23 mg/g for Cd2+, Cu2+, RY 39, and AB 225, respectively. Calculated thermodynamic parameters suggested that the adsorption of all pollutants is spontaneous and of endothermic character. Moreover, different binary mixtures were prepared, and the competitive adsorptions revealed that the soybean hulls are the most efficient adsorbent for the mixture of AB 225 and Cu2+. The findings of this study contribute to the soybean hulls' recovery after the peroxidase extraction and bring them into the circular economy concept.
PB  - Hindawi Limited
T2  - Adsorption Science and Technology
T1  - Recovering the Soybean Hulls after Peroxidase Extraction and Their Application as Adsorbent for Metal Ions and Dyes
SP  - 8532316
VL  - 2023
DO  - 10.1155/2023/8532316
ER  - 

@article{
author = "Ivanovska, Aleksandra and Dojčinović, Biljana and Lađarević, Jelena and Pavun, Leposava and Mijin, Dušan and Kostić, Mirjana and Svetozarević, Milica",
year = "2023",
abstract = "This study is aimed at extending the soybean hulls' lifetime by their utilization as an adsorbent for metal ions (Cd2+ and Cu2+) and dyes (Reactive Yellow 39 (RY 39) and Acid Blue 225 (AB 225)). ATR-FTIR spectroscopy, FE-SEM microscopy, and zeta potential measurements were used for adsorbent characterization. The effect of the solution's pH, peroxidase extraction, adsorbent particle size, contact time, the pollutant's initial concentration, and temperature on the soybean hulls' adsorption potential was studied. Before peroxidase extraction, soybean hulls were capable of removing 72% Cd2+, 71% Cu2+ (at a pH of 5.00) or 81% RY 39, and 73% AB 225 (at a pH of 3.00). For further experiments, soybean hulls without peroxidase were used for several reasons: (1) due to their observed higher metal ion removal, (2) in order to reduce the waste disposal cost after the peroxidase (usually used for wastewater decolorization) extraction, and (3) since the soybean hulls without peroxidase possessed significantly lower secondary pollution than those with peroxidase. Cd2+ and Cu2+ removal was slightly increased when the smaller adsorbent fraction (710-1000 μm) was used, while the adsorbent particle size did not have an impact on dye removal. After 30 min of contact time, 92% and 88% of RY 39 and AB 225 were removed, respectively, while after the same contact time, 80% and 69% of Cd2+ and Cu2+ were removed, respectively. Adsorption of all tested pollutants follows a pseudo-second-order reaction through the fast adsorption, intraparticle diffusion, and final equilibrium stage. The maximal adsorption capacities determined by the Langmuir model were 21.10, 20.54, 16.54, and 17.23 mg/g for Cd2+, Cu2+, RY 39, and AB 225, respectively. Calculated thermodynamic parameters suggested that the adsorption of all pollutants is spontaneous and of endothermic character. Moreover, different binary mixtures were prepared, and the competitive adsorptions revealed that the soybean hulls are the most efficient adsorbent for the mixture of AB 225 and Cu2+. The findings of this study contribute to the soybean hulls' recovery after the peroxidase extraction and bring them into the circular economy concept.",
publisher = "Hindawi Limited",
journal = "Adsorption Science and Technology",
title = "Recovering the Soybean Hulls after Peroxidase Extraction and Their Application as Adsorbent for Metal Ions and Dyes",
pages = "8532316",
volume = "2023",
doi = "10.1155/2023/8532316"
}

Ivanovska, A., Dojčinović, B., Lađarević, J., Pavun, L., Mijin, D., Kostić, M.,& Svetozarević, M.. (2023). Recovering the Soybean Hulls after Peroxidase Extraction and Their Application as Adsorbent for Metal Ions and Dyes. in Adsorption Science and Technology
Hindawi Limited., 2023, 8532316.
https://doi.org/10.1155/2023/8532316

Ivanovska A, Dojčinović B, Lađarević J, Pavun L, Mijin D, Kostić M, Svetozarević M. Recovering the Soybean Hulls after Peroxidase Extraction and Their Application as Adsorbent for Metal Ions and Dyes. in Adsorption Science and Technology. 2023;2023:8532316.
doi:10.1155/2023/8532316 .

Ivanovska, Aleksandra, Dojčinović, Biljana, Lađarević, Jelena, Pavun, Leposava, Mijin, Dušan, Kostić, Mirjana, Svetozarević, Milica, "Recovering the Soybean Hulls after Peroxidase Extraction and Their Application as Adsorbent for Metal Ions and Dyes" in Adsorption Science and Technology, 2023 (2023):8532316,
https://doi.org/10.1155/2023/8532316 . .

TY  - CONF
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Svetozarević, Milica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6667
AB  - Deponovanje  otpada  jedan  je  od  elemenata  integrisanog  upravljanja  otpadom.  Valjano  pro-jektovanje bi, između ostalih elemenata, obavezno moralo da podrazumeva i prikupljanje deponij-skog gasa. Deponijski gas (eng. Landfill gas –LFG) nastaje kao proizvod anaerobnog razlaganja biodegradabilnih  komponenti  ostataka  komunalnog  otpada  na deponiji i prosečno sadrži 50-55% metana  (CH4)  i  45-50%  ugljen-dioksida  (CO2). Energetska vrednost deponijskog gasa kreće se u rasponu 46-30 MJ/m3. Pored energetskog potencijala deponijski gas je veoma važan sa stanovišta zaštite životne sredine i važno jeiskoristiti deponijski gas pre nego što se deponija potpuno zatvori. Rad govori o mogućnosti iskorišćenja potencijala deponijskog gasa pre njenog zatvaranja.
AB  - Waste disposal is an important element of integrated waste management. It is a necessary step although it could significantly affect the environment and the goal is to do everything to protect it. Collection of landfill gas (LFG)should be included the in proper landfill design. Landfill gas, as a product of the anaerobic decomposition of biodegradable components from municipal waste at the landfill, contains on average 50-55% methane (CH4) and 45-50% carbon dioxide (CO2). The energy value of landfill gas ranges from 46 to 30 MJ/m3. The paper is about the possibility of utilizing the potential of landfill gas before its closure.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac
T1  - Iskorišćenje potencijala deponijskog gasa u slučaju parcijalnog zatvaranja deponije
T1  - The potential of landfill gas utilization for the case of partial closure of the sanitary landfill
EP  - 81
SP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6667
ER  - 

@conference{
author = "Dajić, Ana and Mihajlović, Marina and Svetozarević, Milica",
year = "2023",
abstract = "Deponovanje  otpada  jedan  je  od  elemenata  integrisanog  upravljanja  otpadom.  Valjano  pro-jektovanje bi, između ostalih elemenata, obavezno moralo da podrazumeva i prikupljanje deponij-skog gasa. Deponijski gas (eng. Landfill gas –LFG) nastaje kao proizvod anaerobnog razlaganja biodegradabilnih  komponenti  ostataka  komunalnog  otpada  na deponiji i prosečno sadrži 50-55% metana  (CH4)  i  45-50%  ugljen-dioksida  (CO2). Energetska vrednost deponijskog gasa kreće se u rasponu 46-30 MJ/m3. Pored energetskog potencijala deponijski gas je veoma važan sa stanovišta zaštite životne sredine i važno jeiskoristiti deponijski gas pre nego što se deponija potpuno zatvori. Rad govori o mogućnosti iskorišćenja potencijala deponijskog gasa pre njenog zatvaranja., Waste disposal is an important element of integrated waste management. It is a necessary step although it could significantly affect the environment and the goal is to do everything to protect it. Collection of landfill gas (LFG)should be included the in proper landfill design. Landfill gas, as a product of the anaerobic decomposition of biodegradable components from municipal waste at the landfill, contains on average 50-55% methane (CH4) and 45-50% carbon dioxide (CO2). The energy value of landfill gas ranges from 46 to 30 MJ/m3. The paper is about the possibility of utilizing the potential of landfill gas before its closure.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac",
title = "Iskorišćenje potencijala deponijskog gasa u slučaju parcijalnog zatvaranja deponije, The potential of landfill gas utilization for the case of partial closure of the sanitary landfill",
pages = "81-77",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6667"
}

Dajić, A., Mihajlović, M.,& Svetozarević, M.. (2023). Iskorišćenje potencijala deponijskog gasa u slučaju parcijalnog zatvaranja deponije. in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 77-81.
https://hdl.handle.net/21.15107/rcub_technorep_6667

Dajić A, Mihajlović M, Svetozarević M. Iskorišćenje potencijala deponijskog gasa u slučaju parcijalnog zatvaranja deponije. in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac. 2023;:77-81.
https://hdl.handle.net/21.15107/rcub_technorep_6667 .

Dajić, Ana, Mihajlović, Marina, Svetozarević, Milica, "Iskorišćenje potencijala deponijskog gasa u slučaju parcijalnog zatvaranja deponije" in Zbornik radova / 36. Međunarodni kongres o procesnoj industriji, PROCESING '23, 1 i 2. jun 2023, Šabac (2023):77-81,
https://hdl.handle.net/21.15107/rcub_technorep_6667 .

TY  - CONF
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6652
AB  - Enzyme immobilization is a convenient technique for reuse of enzymes –it is one of the advantages that contributes for enhanced productivity and efficacy of these processes. Enzyme stability and their recovery from a reaction mixture are just a couple of the many benefits that can be acquired by immobilization [1]. These features give opportunity for enzymatic application at industrial scale. The enzyme used in this study is perox-idase, an oxidoreductase that oxidizes variety of organic pollutants such as: phenols, textile dyes and phar-maceutically active compounds[2]. The use of potato peel as a source of peroxidase for degradation of textile dye contributes to the  sustainability  of the treatment. The simple isolation of peroxidase as  a crude extract adds up to the cost effectiveness of the product (peroxidase).Given the fact that peroxidase is an eco-friendly biocatalyst, an effort is made to find a suitable ‘green’ way of immobilization. Glutaraldehyde is the most common cross-linker used for the same kind of immobilization [3]. However, it is a toxic compound, so it is replaced by alternative compounds such as natural polysaccharides. One of them is pectin that can be oxidized in order to introduce aldehyde groups, which in turn react with the amino-groups from the amino-acid residues and form Schiff bases [4], [5]. Afterwards, the cross-linked peroxidase can be applied for oxidation reaction.In this study, peroxidase was isolated from waste material –potato peel as a crude extract. The enzy-matic crude extract was precipitated with different reagents, in order to find the most suitable one. Next, the influence of the cross-linker concentration was examined. The cross-linked potato peel peroxidase was used for  biodegradation  of  the  anthraquinone  dye  Acid  Violet  109.  The  process  parameters:  pH,  reaction  time, enzyme, hydrogen peroxide and dye concentration were optimized for achieving the maximal degradation rate. The operational stability, as a key parameter in immobilized systems was also examined.
AB  - Boje koje se koriste u tekstilnoj industriji predstavljaju opasnost po životnu sredinu zbog toga što veći deo na kraju završi u vodotokovima. Kako konvencionalne tretmane karakteriše potreba za velikim količinama reagenasa, visoki troškovi procesa kao i pojava značajnih količina otpadnih materija na kraju procesa, stalno se radi na pronalasku novih efikasnijih tretmana otpadne vode. Za razliku od klasičnih tretmana, primena enzima, kao ekološki benignih biokatalizatora, predstavljaju „zeleno“ rešenje ovog problema. Kako bi se smanjili troškovi proizvodnje enzima predložena je njihova izolacija iz otpadnih materijala. Dugotrajni, komplikovani postupci prečišćavanja otpadnih materijala mogli bi se zaobići izolacijom u obliku sirovog ekstrakta. Imobilizacija enzima omogućila bi njihovu višekratnu upotrebu što bi doprinelo efikasnosti procesa. U izvedenim eksperimentima, ispitan je uticaj taložnog reagensa i koncentracije sredstva za umrežavanje na aktivnost enzima i efikasnost imobilizacije. Nakon umrežavanja, enzimski agregati su su korišćeni za razgradnju boje Acid Violet 109 i optimizovani su sledeći parametri: pH, koncentracija enzima, koncentracija vodonik-peroksida i koncentracija boje. Tokom eksperimenata ispitana je i operativna stabilnost umreženih agregartata.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
T1  - To be free or not to be free: cross-linking of potato peel peroxidase for “green” degradation of anthraquinone dye
T1  - Imobilizacija peroksidaze iz krompirovih ljuski u obliku umreženih enzimskih agregata za “zelenu” razgradnju antrahinonske boje
EP  - 146
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6652
ER  - 

@conference{
author = "Svetozarević, Milica and Šekuljica, Nataša and Dajić, Ana and Mihajlović, Marina and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2022",
abstract = "Enzyme immobilization is a convenient technique for reuse of enzymes –it is one of the advantages that contributes for enhanced productivity and efficacy of these processes. Enzyme stability and their recovery from a reaction mixture are just a couple of the many benefits that can be acquired by immobilization [1]. These features give opportunity for enzymatic application at industrial scale. The enzyme used in this study is perox-idase, an oxidoreductase that oxidizes variety of organic pollutants such as: phenols, textile dyes and phar-maceutically active compounds[2]. The use of potato peel as a source of peroxidase for degradation of textile dye contributes to the  sustainability  of the treatment. The simple isolation of peroxidase as  a crude extract adds up to the cost effectiveness of the product (peroxidase).Given the fact that peroxidase is an eco-friendly biocatalyst, an effort is made to find a suitable ‘green’ way of immobilization. Glutaraldehyde is the most common cross-linker used for the same kind of immobilization [3]. However, it is a toxic compound, so it is replaced by alternative compounds such as natural polysaccharides. One of them is pectin that can be oxidized in order to introduce aldehyde groups, which in turn react with the amino-groups from the amino-acid residues and form Schiff bases [4], [5]. Afterwards, the cross-linked peroxidase can be applied for oxidation reaction.In this study, peroxidase was isolated from waste material –potato peel as a crude extract. The enzy-matic crude extract was precipitated with different reagents, in order to find the most suitable one. Next, the influence of the cross-linker concentration was examined. The cross-linked potato peel peroxidase was used for  biodegradation  of  the  anthraquinone  dye  Acid  Violet  109.  The  process  parameters:  pH,  reaction  time, enzyme, hydrogen peroxide and dye concentration were optimized for achieving the maximal degradation rate. The operational stability, as a key parameter in immobilized systems was also examined., Boje koje se koriste u tekstilnoj industriji predstavljaju opasnost po životnu sredinu zbog toga što veći deo na kraju završi u vodotokovima. Kako konvencionalne tretmane karakteriše potreba za velikim količinama reagenasa, visoki troškovi procesa kao i pojava značajnih količina otpadnih materija na kraju procesa, stalno se radi na pronalasku novih efikasnijih tretmana otpadne vode. Za razliku od klasičnih tretmana, primena enzima, kao ekološki benignih biokatalizatora, predstavljaju „zeleno“ rešenje ovog problema. Kako bi se smanjili troškovi proizvodnje enzima predložena je njihova izolacija iz otpadnih materijala. Dugotrajni, komplikovani postupci prečišćavanja otpadnih materijala mogli bi se zaobići izolacijom u obliku sirovog ekstrakta. Imobilizacija enzima omogućila bi njihovu višekratnu upotrebu što bi doprinelo efikasnosti procesa. U izvedenim eksperimentima, ispitan je uticaj taložnog reagensa i koncentracije sredstva za umrežavanje na aktivnost enzima i efikasnost imobilizacije. Nakon umrežavanja, enzimski agregati su su korišćeni za razgradnju boje Acid Violet 109 i optimizovani su sledeći parametri: pH, koncentracija enzima, koncentracija vodonik-peroksida i koncentracija boje. Tokom eksperimenata ispitana je i operativna stabilnost umreženih agregartata.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd",
title = "To be free or not to be free: cross-linking of potato peel peroxidase for “green” degradation of anthraquinone dye, Imobilizacija peroksidaze iz krompirovih ljuski u obliku umreženih enzimskih agregata za “zelenu” razgradnju antrahinonske boje",
pages = "146-141",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6652"
}

Svetozarević, M., Šekuljica, N., Dajić, A., Mihajlović, M., Knežević-Jugović, Z.,& Mijin, D.. (2022). To be free or not to be free: cross-linking of potato peel peroxidase for “green” degradation of anthraquinone dye. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 141-146.
https://hdl.handle.net/21.15107/rcub_technorep_6652

Svetozarević M, Šekuljica N, Dajić A, Mihajlović M, Knežević-Jugović Z, Mijin D. To be free or not to be free: cross-linking of potato peel peroxidase for “green” degradation of anthraquinone dye. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd. 2022;:141-146.
https://hdl.handle.net/21.15107/rcub_technorep_6652 .

Svetozarević, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Knežević-Jugović, Zorica, Mijin, Dušan, "To be free or not to be free: cross-linking of potato peel peroxidase for “green” degradation of anthraquinone dye" in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd (2022):141-146,
https://hdl.handle.net/21.15107/rcub_technorep_6652 .

TY  - CONF
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Onjia, Antonije
AU  - Barać, Nemanja
AU  - Mihajlović, Marina
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6653
AB  - Uklanjanje boja iz otpadnih voda je jedan od glavnih interesovanja naučne zajednice koja
se bavi zaštitom životne sredine. U ekološko prihvatljive metode za prečišćavanje otpadnih
voda spadaju i enzimski tretmani, među kojima je i oksidacija boja peroksidazom. Visoka
tržišna cena prečišćenih enzima dovela je do izolovanja enzima iz jeftinih izvora i do
primene enzima u obliku sirovog ekstrakta. Izvođenje kontinualnog sistema doprinosi
povećanoj efikasnosti procesa. U ovom radu, izolovana je peroksidaza iz otpadnog
materijala – krompirovih ljuski za obezbojavanje boje Acid Violet 109. Uklanjanje je
izvršeno u kontinualnom mikroreaktoru. Optimizovani su glavni procesni parametri: vreme
zadržavanja, aktivnost enzima, koncentracija vodonik-peroksida i boje, kao i prečnik i
dužina reaktora. Nakon vremena zadržavanja od 3 min u mikroreaktoru prečnika 0,5 mm i
dužine 6m, procenat obezbojavanje je iznosio 76%.
AB  - Dye removal from wastewater is one of the major interests of the scientific community
dealing with environmental protection. Among available techniques for wastewater
decolorization is dye oxidation using peroxidase. Taking into consideration the high
market price of purified enzymes, the isolation of enzymes from cheap sources and the
potential use of crude enzyme extractscontributes to the sustainabilityof the treatment.
Furthermore, introducing continuous flow systems enhances the process's productivity and
efficiency. In this study, peroxidase was isolated from waste material –potato peel and
used for removal of anthraquinone dye Acid Violet 109 in a continuous flow. The main
process parameters were optimized: residence time, enzyme activity, hydrogen peroxide
and dye concentration, as well as the reactor's length and diameter. After 3min of residence
time, in a microreactor with 0.5 mm diameter and 6m length, the decolorization rate was
76%.
PB  - Beograd : Srpsko hemijsko društvo
C3  - Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine
T1  - Biokataliza u mikroreaktoru: enzimsko obezbojavanje tekstilne boje
T1  - Continuous flowbiocatalysis: enzymatic decolorization of textile dye
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6653
ER  - 

@conference{
author = "Svetozarević, Milica and Šekuljica, Nataša and Onjia, Antonije and Barać, Nemanja and Mihajlović, Marina and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2022",
abstract = "Uklanjanje boja iz otpadnih voda je jedan od glavnih interesovanja naučne zajednice koja
se bavi zaštitom životne sredine. U ekološko prihvatljive metode za prečišćavanje otpadnih
voda spadaju i enzimski tretmani, među kojima je i oksidacija boja peroksidazom. Visoka
tržišna cena prečišćenih enzima dovela je do izolovanja enzima iz jeftinih izvora i do
primene enzima u obliku sirovog ekstrakta. Izvođenje kontinualnog sistema doprinosi
povećanoj efikasnosti procesa. U ovom radu, izolovana je peroksidaza iz otpadnog
materijala – krompirovih ljuski za obezbojavanje boje Acid Violet 109. Uklanjanje je
izvršeno u kontinualnom mikroreaktoru. Optimizovani su glavni procesni parametri: vreme
zadržavanja, aktivnost enzima, koncentracija vodonik-peroksida i boje, kao i prečnik i
dužina reaktora. Nakon vremena zadržavanja od 3 min u mikroreaktoru prečnika 0,5 mm i
dužine 6m, procenat obezbojavanje je iznosio 76%., Dye removal from wastewater is one of the major interests of the scientific community
dealing with environmental protection. Among available techniques for wastewater
decolorization is dye oxidation using peroxidase. Taking into consideration the high
market price of purified enzymes, the isolation of enzymes from cheap sources and the
potential use of crude enzyme extractscontributes to the sustainabilityof the treatment.
Furthermore, introducing continuous flow systems enhances the process's productivity and
efficiency. In this study, peroxidase was isolated from waste material –potato peel and
used for removal of anthraquinone dye Acid Violet 109 in a continuous flow. The main
process parameters were optimized: residence time, enzyme activity, hydrogen peroxide
and dye concentration, as well as the reactor's length and diameter. After 3min of residence
time, in a microreactor with 0.5 mm diameter and 6m length, the decolorization rate was
76%.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine",
title = "Biokataliza u mikroreaktoru: enzimsko obezbojavanje tekstilne boje, Continuous flowbiocatalysis: enzymatic decolorization of textile dye",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6653"
}

Svetozarević, M., Šekuljica, N., Onjia, A., Barać, N., Mihajlović, M., Knežević-Jugović, Z.,& Mijin, D.. (2022). Biokataliza u mikroreaktoru: enzimsko obezbojavanje tekstilne boje. in Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine
Beograd : Srpsko hemijsko društvo., 81.
https://hdl.handle.net/21.15107/rcub_technorep_6653

Svetozarević M, Šekuljica N, Onjia A, Barać N, Mihajlović M, Knežević-Jugović Z, Mijin D. Biokataliza u mikroreaktoru: enzimsko obezbojavanje tekstilne boje. in Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine. 2022;:81.
https://hdl.handle.net/21.15107/rcub_technorep_6653 .

Svetozarević, Milica, Šekuljica, Nataša, Onjia, Antonije, Barać, Nemanja, Mihajlović, Marina, Knežević-Jugović, Zorica, Mijin, Dušan, "Biokataliza u mikroreaktoru: enzimsko obezbojavanje tekstilne boje" in Kratki izvodi radova ; Knjiga radova / 58. savetovanje Srpskog hemijskog društva, Beograd, 9. i 10. jun 2022. godine (2022):81,
https://hdl.handle.net/21.15107/rcub_technorep_6653 .

TY  - THES
AU  - Svetozarević, Milica
PY  - 2022
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9086
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:29321/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/115388681
UR  - https://nardus.mpn.gov.rs/handle/123456789/21416
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6665
AB  - Ideja ove doktorske disertacije je izolovanje peroksidaze iz otpadnog materijala i njena upotreba urazgradnji antrahinonske boje Kiselo ljubičasto 109 (Acid Violet 109, AV109). Enzim je izolovaniz otpadnog materijala u cilju povećanje ekonomičnosti i održivosti procesa. Razmotreni su šaržni ikontinualni sistemi u cilju optimizacije razgradnje boje.U prvom delu disertacije ispitivana je mogućnost izolovanja peroksidaze iz različitog otpadnogmaterijala i odabir najpogodnijeg izvora. Potom, je izvršena optimizacija procesa razgradnje AV109u šaržnom reaktoru. Sa tim u vezi ispitan je uticaj procesnih parametara kao što su: vreme kontakta,pH reakcione smeše, koncentracija enzima, vodonik-peroksida i boje, i temperatura. Zatim, posebnapažnja je posvećena ispitivanju početne kinetike enzimske reakcije i utvrđivanju kinetičkihkonstanti.Sledeći deo disertacije odnosi se na kontinualnu razgradnju antrahinonske boje AV109 umikroreaktorskom sistemu. Kontinualni sistemi generalno pokazuju veću efikasnost, a olakšano je ivođenje procesa. U tu svrhu, optimizovani su parametri koji imaju glavnu ulogu u razgradnjiAV109 u mikroreaktoru: vreme zadržavanja, dužina i prečnik reaktora kao i koncentracijski protokvodonik-peroksida, enzima i boje.Da bi se čitav proces unapredio i postao efikasniji, ,,zeleniji” i ekonomičniji, vršena je imobilizacijaenzima umrežavanjem u kontinualnom i šaržnom reaktoru. Na taj način, bilo je moguće koristitienzim dobijen iz otpadnog materijala više puta. Ova vrsta imobilizacije odvija se u dva koraka:taloženje enzima i umrežavanje. Za umrežavanje enzima korišćen je pektin - prirodni polimerbezbedan za okolinu. Nakon odabira najpogodnijeg taložnog reagensa ispitivan je uticajkoncentracije umreživača na aktivnost enzima. Umreženi enzimski agregati su primenjeni u reakcijibiorazgradnje AV109 u šaržnom reaktoru zapremine 50 mL i optimizovani su sledeći parametri: pHvrednost, koncentracija imobilizata, vodonik-peroksida i boje. Takođe, ispitana je operativnastabilnost umreženih enzimskih agregata.U kontinualnom sistemu, je ispitivana mogućnost imobilizacije enzima na unutrašnjim zidovimamikroreaktora. Imobilizacija peroksidaze u cevnom mikroreaktoru je potvrđena SEM analizom.Dalje, optimizovana je koncentracija umreživača i koncentracija enzima, a ispitivana je i operativnastabilnost u reakciji razgradnje boje AV109.Dobijeni rezultati ukazuju na efikasnu razgradnje boje, ali mehanizam delovanja peroksidaze nijerasvetljen u potpunosti. Zbog toga, izvršena je LC/GC – MS analiza proizvoda razgradnje.Ispitivana je i hemijska potrošnja kiseonika u cilju utvrđivanja toksičnosti sekundarnih proizvodareakcije.
AB  - The idea behind this doctoral thesis is extraction of peroxidase from waste material and its use forbiodegradation of the anthraquinone dye Acid Violet 109. The enzyme is isolated from agro-industrial waste in order to increase the overall cost-effectiveness and sustainability of the process.Batch and continuous systems are considered for dye removal process’s optimization.In the thesis first section, the possibility of peroxidase extraction from different waste material andselection of the most suitable one is examined. Afterwards, optimization of the biodegradationbatch process is carried out. Contact time, pH, enzyme, hydrogen peroxide and dye concentration,temperature, kinetic constants and inhibition constants are optimized.Next section of this doctoral thesis deals with the continuous dye biodegradation in a microreactor.Continuous systems show increased efficiency and easier manipulation of the process. Bearing inmind the latter mentioned characteristics, the key parameters for continuous biodegradation of theAcid Violet 109 dye are optimized: residence time, reactor’s length and diameter, together withprocess parameters: concentration flow of enzyme, hydrogen peroxide and dye.As a means to a more advanced, efficient and greener process, the enzyme is immobilized by cross-linking in the batch and continuous reactor. Thus, the enzyme obtained from a waste material can bereused several times. Pectin is used for enzyme cross-linking. It is a biopolymer that is recognizedas safe for the environment. The effect of the precipitation reagents on the enzyme activity isexamined. After choosing the proper precipitant, the cross-linker concentration is optimized. Thecross-linked enzyme aggregates are applied in the biodegradation of the athraquinone dye in a 50mL batch reactor. The following parameters are optimized: pH, cross-linked enzyme, hydrogenperoxide and dye concentration. The possibility of the cross-linked enzyme aggregates reuse isconfirmed by biodegrading the dye in 10 successive cycles.In continuous flow, the peroxidase is cross-linked onto the inner walls of the PTFE tubemicroreactor. Scanning electron microscopy analysis confirmed the successful outcome of theimmobilization. Afterwards, the enzyme and cross-linker concentration was optimized, togetherwith reusability assessment of the immobilized enzyme microreactor.The obtained results indicate of a successful prosperous dye biodegradation process, but themechanism of the biodegradation is not revealed. Hence, LC/GC-MS analysis of the degradationproducts is performed. Chemical oxygen demand was analyzed as a validation that generation ofsecondary byproducts which are more toxic than the dye itself does not occur.
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T2  - Univerzitet u Beogradu
T1  - Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21416
ER  - 

@phdthesis{
author = "Svetozarević, Milica",
year = "2022",
abstract = "Ideja ove doktorske disertacije je izolovanje peroksidaze iz otpadnog materijala i njena upotreba urazgradnji antrahinonske boje Kiselo ljubičasto 109 (Acid Violet 109, AV109). Enzim je izolovaniz otpadnog materijala u cilju povećanje ekonomičnosti i održivosti procesa. Razmotreni su šaržni ikontinualni sistemi u cilju optimizacije razgradnje boje.U prvom delu disertacije ispitivana je mogućnost izolovanja peroksidaze iz različitog otpadnogmaterijala i odabir najpogodnijeg izvora. Potom, je izvršena optimizacija procesa razgradnje AV109u šaržnom reaktoru. Sa tim u vezi ispitan je uticaj procesnih parametara kao što su: vreme kontakta,pH reakcione smeše, koncentracija enzima, vodonik-peroksida i boje, i temperatura. Zatim, posebnapažnja je posvećena ispitivanju početne kinetike enzimske reakcije i utvrđivanju kinetičkihkonstanti.Sledeći deo disertacije odnosi se na kontinualnu razgradnju antrahinonske boje AV109 umikroreaktorskom sistemu. Kontinualni sistemi generalno pokazuju veću efikasnost, a olakšano je ivođenje procesa. U tu svrhu, optimizovani su parametri koji imaju glavnu ulogu u razgradnjiAV109 u mikroreaktoru: vreme zadržavanja, dužina i prečnik reaktora kao i koncentracijski protokvodonik-peroksida, enzima i boje.Da bi se čitav proces unapredio i postao efikasniji, ,,zeleniji” i ekonomičniji, vršena je imobilizacijaenzima umrežavanjem u kontinualnom i šaržnom reaktoru. Na taj način, bilo je moguće koristitienzim dobijen iz otpadnog materijala više puta. Ova vrsta imobilizacije odvija se u dva koraka:taloženje enzima i umrežavanje. Za umrežavanje enzima korišćen je pektin - prirodni polimerbezbedan za okolinu. Nakon odabira najpogodnijeg taložnog reagensa ispitivan je uticajkoncentracije umreživača na aktivnost enzima. Umreženi enzimski agregati su primenjeni u reakcijibiorazgradnje AV109 u šaržnom reaktoru zapremine 50 mL i optimizovani su sledeći parametri: pHvrednost, koncentracija imobilizata, vodonik-peroksida i boje. Takođe, ispitana je operativnastabilnost umreženih enzimskih agregata.U kontinualnom sistemu, je ispitivana mogućnost imobilizacije enzima na unutrašnjim zidovimamikroreaktora. Imobilizacija peroksidaze u cevnom mikroreaktoru je potvrđena SEM analizom.Dalje, optimizovana je koncentracija umreživača i koncentracija enzima, a ispitivana je i operativnastabilnost u reakciji razgradnje boje AV109.Dobijeni rezultati ukazuju na efikasnu razgradnje boje, ali mehanizam delovanja peroksidaze nijerasvetljen u potpunosti. Zbog toga, izvršena je LC/GC – MS analiza proizvoda razgradnje.Ispitivana je i hemijska potrošnja kiseonika u cilju utvrđivanja toksičnosti sekundarnih proizvodareakcije., The idea behind this doctoral thesis is extraction of peroxidase from waste material and its use forbiodegradation of the anthraquinone dye Acid Violet 109. The enzyme is isolated from agro-industrial waste in order to increase the overall cost-effectiveness and sustainability of the process.Batch and continuous systems are considered for dye removal process’s optimization.In the thesis first section, the possibility of peroxidase extraction from different waste material andselection of the most suitable one is examined. Afterwards, optimization of the biodegradationbatch process is carried out. Contact time, pH, enzyme, hydrogen peroxide and dye concentration,temperature, kinetic constants and inhibition constants are optimized.Next section of this doctoral thesis deals with the continuous dye biodegradation in a microreactor.Continuous systems show increased efficiency and easier manipulation of the process. Bearing inmind the latter mentioned characteristics, the key parameters for continuous biodegradation of theAcid Violet 109 dye are optimized: residence time, reactor’s length and diameter, together withprocess parameters: concentration flow of enzyme, hydrogen peroxide and dye.As a means to a more advanced, efficient and greener process, the enzyme is immobilized by cross-linking in the batch and continuous reactor. Thus, the enzyme obtained from a waste material can bereused several times. Pectin is used for enzyme cross-linking. It is a biopolymer that is recognizedas safe for the environment. The effect of the precipitation reagents on the enzyme activity isexamined. After choosing the proper precipitant, the cross-linker concentration is optimized. Thecross-linked enzyme aggregates are applied in the biodegradation of the athraquinone dye in a 50mL batch reactor. The following parameters are optimized: pH, cross-linked enzyme, hydrogenperoxide and dye concentration. The possibility of the cross-linked enzyme aggregates reuse isconfirmed by biodegrading the dye in 10 successive cycles.In continuous flow, the peroxidase is cross-linked onto the inner walls of the PTFE tubemicroreactor. Scanning electron microscopy analysis confirmed the successful outcome of theimmobilization. Afterwards, the enzyme and cross-linker concentration was optimized, togetherwith reusability assessment of the immobilized enzyme microreactor.The obtained results indicate of a successful prosperous dye biodegradation process, but themechanism of the biodegradation is not revealed. Hence, LC/GC-MS analysis of the degradationproducts is performed. Chemical oxygen demand was analyzed as a validation that generation ofsecondary byproducts which are more toxic than the dye itself does not occur.",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
journal = "Univerzitet u Beogradu",
title = "Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21416"
}

Svetozarević, M.. (2022). Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu. in Univerzitet u Beogradu
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_21416

Svetozarević M. Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu. in Univerzitet u Beogradu. 2022;.
https://hdl.handle.net/21.15107/rcub_nardus_21416 .

Svetozarević, Milica, "Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu" in Univerzitet u Beogradu (2022),
https://hdl.handle.net/21.15107/rcub_nardus_21416 .

TY  - CONF
AU  - Tadić, Julijana
AU  - Gazikalović, Ivana
AU  - Lađarević, Jelena
AU  - Mašulović, Aleksandra
AU  - Svetozarević, Milica
AU  - Porobić, Slavica
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6658
AB  - The heterocyclic azo dyes represent significant synthetic organic colorants. In addition to their exceptional coloration properties, heterocyclic azo compounds exhibit antibacterial, antifungal, an-tiviral, antioxidant, anti-inflammatory and anticancer properties. The antimicrobial resistance is one of the major problems for global public health. Therefore, finding new organic structures with anti-microbial properties represents a challenging research field. In this study, antimicrobial screening of two Biginelli-based azo pyridone dyes has been carried out. The antimicrobial activity of synthe-tized dyes wasstudied against bacteria (Staphylococcus aureus, Escherichia coli) and yeast (Candida albicans), using agar diffusion method. The preliminary research results indicate the antimicrobial potential of the investigated compounds.
AB  - Heterociklična azo jedinjenja predstavljaju značajnu klasnu organskih sintetskih boja. Pored njihovih izuzetnih svojstava za bojenje, heterociklična azo jedinjenja imaju i biološku aktivnost. Mnoge studije pokazuju da azo boje sa heterocikličnim fragmentima u strukturi mogu ispoljavati antibakterijska, antifungalna, antivirusna, antiinflamatorna i antikancerogena svojstva. Rezistencija mikroorganizama na postojeće antimikrobne agense predstavlja jedan od najvećih problema u svetskom zdravstvu. Zbog toga je razvijanje novih molekula sa antimikrobnim svojstvima kako značajno, tako i neophodno za globalno očuvanje zdravlja. U ovom radu, ispitivanje antimikrobne aktivnosti dve Biđineli-azo piridonske boje je urađeno pomoću metode difuzije na agarnoj podlozi. Antimikrobna svojstva su ispitana prema patogenim sojevima Staphylococcus aureus (Gram-pozitivna bakterija), Escherichia coli (Gram-negativna bakterija) i Candida albicans (oportunistički kvasac). Preliminarni rezultati istraživanja ukazali su na antimikrobni potencijal ispitivanih azo jedinjenja.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
T1  - The antimicrobial evaluation of some biginelli-based azo pyridone dyes
T1  - Ispitivanje antimikrobnih svojstava nekih biđineli-azo piridonskih boja
EP  - 28
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6658
ER  - 

@conference{
author = "Tadić, Julijana and Gazikalović, Ivana and Lađarević, Jelena and Mašulović, Aleksandra and Svetozarević, Milica and Porobić, Slavica and Mijin, Dušan",
year = "2022",
abstract = "The heterocyclic azo dyes represent significant synthetic organic colorants. In addition to their exceptional coloration properties, heterocyclic azo compounds exhibit antibacterial, antifungal, an-tiviral, antioxidant, anti-inflammatory and anticancer properties. The antimicrobial resistance is one of the major problems for global public health. Therefore, finding new organic structures with anti-microbial properties represents a challenging research field. In this study, antimicrobial screening of two Biginelli-based azo pyridone dyes has been carried out. The antimicrobial activity of synthe-tized dyes wasstudied against bacteria (Staphylococcus aureus, Escherichia coli) and yeast (Candida albicans), using agar diffusion method. The preliminary research results indicate the antimicrobial potential of the investigated compounds., Heterociklična azo jedinjenja predstavljaju značajnu klasnu organskih sintetskih boja. Pored njihovih izuzetnih svojstava za bojenje, heterociklična azo jedinjenja imaju i biološku aktivnost. Mnoge studije pokazuju da azo boje sa heterocikličnim fragmentima u strukturi mogu ispoljavati antibakterijska, antifungalna, antivirusna, antiinflamatorna i antikancerogena svojstva. Rezistencija mikroorganizama na postojeće antimikrobne agense predstavlja jedan od najvećih problema u svetskom zdravstvu. Zbog toga je razvijanje novih molekula sa antimikrobnim svojstvima kako značajno, tako i neophodno za globalno očuvanje zdravlja. U ovom radu, ispitivanje antimikrobne aktivnosti dve Biđineli-azo piridonske boje je urađeno pomoću metode difuzije na agarnoj podlozi. Antimikrobna svojstva su ispitana prema patogenim sojevima Staphylococcus aureus (Gram-pozitivna bakterija), Escherichia coli (Gram-negativna bakterija) i Candida albicans (oportunistički kvasac). Preliminarni rezultati istraživanja ukazali su na antimikrobni potencijal ispitivanih azo jedinjenja.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd",
title = "The antimicrobial evaluation of some biginelli-based azo pyridone dyes, Ispitivanje antimikrobnih svojstava nekih biđineli-azo piridonskih boja",
pages = "28-25",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6658"
}

Tadić, J., Gazikalović, I., Lađarević, J., Mašulović, A., Svetozarević, M., Porobić, S.,& Mijin, D.. (2022). The antimicrobial evaluation of some biginelli-based azo pyridone dyes. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 25-28.
https://hdl.handle.net/21.15107/rcub_technorep_6658

Tadić J, Gazikalović I, Lađarević J, Mašulović A, Svetozarević M, Porobić S, Mijin D. The antimicrobial evaluation of some biginelli-based azo pyridone dyes. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd. 2022;:25-28.
https://hdl.handle.net/21.15107/rcub_technorep_6658 .

Tadić, Julijana, Gazikalović, Ivana, Lađarević, Jelena, Mašulović, Aleksandra, Svetozarević, Milica, Porobić, Slavica, Mijin, Dušan, "The antimicrobial evaluation of some biginelli-based azo pyridone dyes" in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd (2022):25-28,
https://hdl.handle.net/21.15107/rcub_technorep_6658 .

TY  - CONF
AU  - Mašulović, Aleksandra
AU  - Lađarević, Jelena
AU  - Veruševski, Vanja
AU  - Matović, Luka
AU  - Svetozarević, Milica
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6657
AB  - Considering a significant antioxidant activity of 6-hydroxy-6-methyl-2-pyridones, in this paper four series of arylazo pyridone dyes were subjected to antioxidant ABTS assay. Firstly, preliminary tests were conducted at concentration of 1 mg/ml in order to investigate the effects of different substituents on the dye activity. Based on the preliminary results, dyes with the highest activity were selected for further evaluation of IC50 values and these values were compared with IC50 value of ascorbic acid used as standard. It has been shown that five arylazo pyridone dyes exhibit more potent activity when compared to the ascorbic acid.
AB  - Uzimajući u obzir značajnu antioksidativnu aktivnost 6-hidroksi-4-metil-2-piridona, u okviru ovog rada ispitana je antioksidativna aktivnost četiri serije arilazo piridonskih azo boja pomoću ABTS testa. U prvom delu su urađeni preliminari testovi aktivnosti pri koncentracij od 1 mg/ml kako bi se ispitao uticaj različitih supstituenata na antioksidativnu aktivnost. Na osnovu ovih rezultata izabrane su boje sa najvećom antioksidativnom aktivnošću, i u drugom delu je za njih određena IC50 vrednost i upoređena sa vrednošću askorbinske kiseline koja je korišćena kao standard. Rezultati su pokazali da pet boja imaju značajniju aktivnost od askorbinske kiseline.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
T1  - Investigation of antioxidant activity of 6-hydroxy-6-methyl-2-pyridones
T1  - Ispitivanje antioksidativne aktivnosti azo boja na bazi 6--hidroksi-4-metil-2-piridona
EP  - 40
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6657
ER  - 

@conference{
author = "Mašulović, Aleksandra and Lađarević, Jelena and Veruševski, Vanja and Matović, Luka and Svetozarević, Milica and Mijin, Dušan",
year = "2022",
abstract = "Considering a significant antioxidant activity of 6-hydroxy-6-methyl-2-pyridones, in this paper four series of arylazo pyridone dyes were subjected to antioxidant ABTS assay. Firstly, preliminary tests were conducted at concentration of 1 mg/ml in order to investigate the effects of different substituents on the dye activity. Based on the preliminary results, dyes with the highest activity were selected for further evaluation of IC50 values and these values were compared with IC50 value of ascorbic acid used as standard. It has been shown that five arylazo pyridone dyes exhibit more potent activity when compared to the ascorbic acid., Uzimajući u obzir značajnu antioksidativnu aktivnost 6-hidroksi-4-metil-2-piridona, u okviru ovog rada ispitana je antioksidativna aktivnost četiri serije arilazo piridonskih azo boja pomoću ABTS testa. U prvom delu su urađeni preliminari testovi aktivnosti pri koncentracij od 1 mg/ml kako bi se ispitao uticaj različitih supstituenata na antioksidativnu aktivnost. Na osnovu ovih rezultata izabrane su boje sa najvećom antioksidativnom aktivnošću, i u drugom delu je za njih određena IC50 vrednost i upoređena sa vrednošću askorbinske kiseline koja je korišćena kao standard. Rezultati su pokazali da pet boja imaju značajniju aktivnost od askorbinske kiseline.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd",
title = "Investigation of antioxidant activity of 6-hydroxy-6-methyl-2-pyridones, Ispitivanje antioksidativne aktivnosti azo boja na bazi 6--hidroksi-4-metil-2-piridona",
pages = "40-37",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6657"
}

Mašulović, A., Lađarević, J., Veruševski, V., Matović, L., Svetozarević, M.,& Mijin, D.. (2022). Investigation of antioxidant activity of 6-hydroxy-6-methyl-2-pyridones. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 37-40.
https://hdl.handle.net/21.15107/rcub_technorep_6657

Mašulović A, Lađarević J, Veruševski V, Matović L, Svetozarević M, Mijin D. Investigation of antioxidant activity of 6-hydroxy-6-methyl-2-pyridones. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd. 2022;:37-40.
https://hdl.handle.net/21.15107/rcub_technorep_6657 .

Mašulović, Aleksandra, Lađarević, Jelena, Veruševski, Vanja, Matović, Luka, Svetozarević, Milica, Mijin, Dušan, "Investigation of antioxidant activity of 6-hydroxy-6-methyl-2-pyridones" in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd (2022):37-40,
https://hdl.handle.net/21.15107/rcub_technorep_6657 .

TY  - CONF
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Popovski, Z.
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5749
AB  - Despite the obvious benefits from mass production such as an increased productivity, lower product cost and rapid evolution, nowadays we are dealing with energy consumption issues and pollution. The generated waste poses a threat to the environment, so new green techniques are developed constantly. Waste valorization is one of the trending concepts that is a part of sustainability strategies. Potato exploitation due to mass production of chips, hash browns, frozen food and starch leads to a formation of high waste load. There are several available ways for potato peel valorization: as a biofertilizer, as a substrate for microbial growth, as an adsorbent, for extraction of antioxidants and for extraction of enzymes. The enzyme peroxidase is abundant in potato peel. This enzyme uses hydrogen peroxide as an activator and can be readily used for oxidation of different compounds - pollutants. In this study, peroxidase was isolated from potato peel and immobilized as cross-linked enzyme aggregates. Pectin was used as a green cross-linker. The immobilized potato peel peroxidase was used for degradation of a textile anthraquinone dye Lanaset Violet B. Under the optimal process parameters: pH 3, 0.4 mM hydrogen peroxide, 0.8 μmol/min CLEA peroxidase, 10 mg/L dye and 70 min, 85.71±1.45 % dye degradation was achieved. The operational stability, as a key parameter for immobilized enzyme systems, was also examined. After 4 cycles CLEA peroxidase kept 31.57±1.79 % of its biodegradation efficiency.
PB  - Institute of Physics
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Cross-linking the peroxidase: From potato peel valorization to colored effluents treatment
IS  - 1
SP  - 012005
VL  - 1123
DO  - 10.1088/1755-1315/1123/1/012005
ER  - 

@conference{
author = "Svetozarević, Milica and Šekuljica, Nataša and Dajić, Ana and Mihajlović, Marina and Popovski, Z. and Mijin, Dušan",
year = "2022",
abstract = "Despite the obvious benefits from mass production such as an increased productivity, lower product cost and rapid evolution, nowadays we are dealing with energy consumption issues and pollution. The generated waste poses a threat to the environment, so new green techniques are developed constantly. Waste valorization is one of the trending concepts that is a part of sustainability strategies. Potato exploitation due to mass production of chips, hash browns, frozen food and starch leads to a formation of high waste load. There are several available ways for potato peel valorization: as a biofertilizer, as a substrate for microbial growth, as an adsorbent, for extraction of antioxidants and for extraction of enzymes. The enzyme peroxidase is abundant in potato peel. This enzyme uses hydrogen peroxide as an activator and can be readily used for oxidation of different compounds - pollutants. In this study, peroxidase was isolated from potato peel and immobilized as cross-linked enzyme aggregates. Pectin was used as a green cross-linker. The immobilized potato peel peroxidase was used for degradation of a textile anthraquinone dye Lanaset Violet B. Under the optimal process parameters: pH 3, 0.4 mM hydrogen peroxide, 0.8 μmol/min CLEA peroxidase, 10 mg/L dye and 70 min, 85.71±1.45 % dye degradation was achieved. The operational stability, as a key parameter for immobilized enzyme systems, was also examined. After 4 cycles CLEA peroxidase kept 31.57±1.79 % of its biodegradation efficiency.",
publisher = "Institute of Physics",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Cross-linking the peroxidase: From potato peel valorization to colored effluents treatment",
number = "1",
pages = "012005",
volume = "1123",
doi = "10.1088/1755-1315/1123/1/012005"
}

Svetozarević, M., Šekuljica, N., Dajić, A., Mihajlović, M., Popovski, Z.,& Mijin, D.. (2022). Cross-linking the peroxidase: From potato peel valorization to colored effluents treatment. in IOP Conference Series: Earth and Environmental Science
Institute of Physics., 1123(1), 012005.
https://doi.org/10.1088/1755-1315/1123/1/012005

Svetozarević M, Šekuljica N, Dajić A, Mihajlović M, Popovski Z, Mijin D. Cross-linking the peroxidase: From potato peel valorization to colored effluents treatment. in IOP Conference Series: Earth and Environmental Science. 2022;1123(1):012005.
doi:10.1088/1755-1315/1123/1/012005 .

Svetozarević, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Popovski, Z., Mijin, Dušan, "Cross-linking the peroxidase: From potato peel valorization to colored effluents treatment" in IOP Conference Series: Earth and Environmental Science, 1123, no. 1 (2022):012005,
https://doi.org/10.1088/1755-1315/1123/1/012005 . .

TY  - JOUR
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Onjia, Antonije
AU  - Barać, Nemanja
AU  - Mihajlović, Marina
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4729
AB  - This paper aims to reduce the gap between intensified innovative process designs and the application of enzyme technologies at an industrial scale that often take full advantage of microreactor systems. Soybean and potato processing waste was used as the source of peroxidase in both the free and immobilized form for the biodegradation of the Acid Violet 109 anthraquinone dye (AV109) in a PTFE (polytetrafluoroethylene) microreactor which is 0.5 mm in diameter and 6 m in length. High biodegradation efficiencies of 95 and 76% were achieved by soybean and potato peroxidase respectively after 3 min of residence in the microreactor, with a noticeable lack of hydrogen peroxide inhibition. The reactor’s design, with regard to its diameter and length, significantly affected the biodegradation efficiency. The polymer membrane formation by the cross-linking approach with oxidized pectin and soybean or potato peel peroxidase on the inner PTFE wall was SEM confirmed. The cross-linking efficiency with oxidized pectin of both peroxidases is inversely correlated with the activity, hence the highest recorded activity of immobilized peroxidase was 11 and 19μmol/min of soybean and potato peroxidase, respectively, at an initial pectin concentration of 0.05%. A promising reusability for AV109 biodegradation in the microreactor with immobilized soybean and potato peroxidase was confirmed, since the immobilized peroxidases retained 65 and 35% of the initial activity after the tenth cycle, respectively. The LC–MS and GC–MS techniques confirmed the biodegradation of the AV 109 dye, and the intermediates with a lower molecular weight were separated and identified.
PB  - Elsevier B.V.
T2  - Environmental Technology & Innovation
T1  - Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor
SP  - 102373
VL  - 26
DO  - 10.1016/j.eti.2022.102373
ER  - 

@article{
author = "Svetozarević, Milica and Šekuljica, Nataša and Onjia, Antonije and Barać, Nemanja and Mihajlović, Marina and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2022",
abstract = "This paper aims to reduce the gap between intensified innovative process designs and the application of enzyme technologies at an industrial scale that often take full advantage of microreactor systems. Soybean and potato processing waste was used as the source of peroxidase in both the free and immobilized form for the biodegradation of the Acid Violet 109 anthraquinone dye (AV109) in a PTFE (polytetrafluoroethylene) microreactor which is 0.5 mm in diameter and 6 m in length. High biodegradation efficiencies of 95 and 76% were achieved by soybean and potato peroxidase respectively after 3 min of residence in the microreactor, with a noticeable lack of hydrogen peroxide inhibition. The reactor’s design, with regard to its diameter and length, significantly affected the biodegradation efficiency. The polymer membrane formation by the cross-linking approach with oxidized pectin and soybean or potato peel peroxidase on the inner PTFE wall was SEM confirmed. The cross-linking efficiency with oxidized pectin of both peroxidases is inversely correlated with the activity, hence the highest recorded activity of immobilized peroxidase was 11 and 19μmol/min of soybean and potato peroxidase, respectively, at an initial pectin concentration of 0.05%. A promising reusability for AV109 biodegradation in the microreactor with immobilized soybean and potato peroxidase was confirmed, since the immobilized peroxidases retained 65 and 35% of the initial activity after the tenth cycle, respectively. The LC–MS and GC–MS techniques confirmed the biodegradation of the AV 109 dye, and the intermediates with a lower molecular weight were separated and identified.",
publisher = "Elsevier B.V.",
journal = "Environmental Technology & Innovation",
title = "Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor",
pages = "102373",
volume = "26",
doi = "10.1016/j.eti.2022.102373"
}

Svetozarević, M., Šekuljica, N., Onjia, A., Barać, N., Mihajlović, M., Knežević-Jugović, Z.,& Mijin, D.. (2022). Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor. in Environmental Technology & Innovation
Elsevier B.V.., 26, 102373.
https://doi.org/10.1016/j.eti.2022.102373

Svetozarević M, Šekuljica N, Onjia A, Barać N, Mihajlović M, Knežević-Jugović Z, Mijin D. Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor. in Environmental Technology & Innovation. 2022;26:102373.
doi:10.1016/j.eti.2022.102373 .

Svetozarević, Milica, Šekuljica, Nataša, Onjia, Antonije, Barać, Nemanja, Mihajlović, Marina, Knežević-Jugović, Zorica, Mijin, Dušan, "Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor" in Environmental Technology & Innovation, 26 (2022):102373,
https://doi.org/10.1016/j.eti.2022.102373 . .

TY  - CONF
AU  - Dajić, Ana
AU  - Mihajlović, Marina
AU  - Svetozarević, Milica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6656
AB  - Porast industrijske aktivnosti za posledicu ima sve veću potrošnju vode a samim tim i sve veće količine otpadne vode opterećene značajnim količinama zagađujućih materija. U vreme kada je zaštita životne sredine aktuelna tema, tehnike prečišćavanja se neprestano unapređuju. Relativno nov koncept prerade otpadne vode uključuje tretmane u mikroreaktorskim sistemima kao atraktivnu alternativu standardnim procesima prečišćavanja. Kako postoji veliki broj različitih tipova mikroreaktorskih sistema i veliki broj metoda uklanjanja zagađujućih materija, otvaraju se nove mogućnosti primene različitih tretmana obrade. U radu je dat pregled tretmana industrijskih otpadnih voda koje je moguće primeniti u mikroreaktorskim sistemima.
AB  - The increase in industrial activity results in increasing water consumption and increasing amounts of wastewater burdened with significant amounts of pollutants. Environmental protection has become an important topic and wastewater treatment techniques are constantly being improved. A relatively new concept includes treatments in microreactor systems as an attractive alternative to standard processes. Large number of different types of microreactor systems and large number of methods for removing pollutants opens up, new possibilities for the application of different treatment types. The paper gives an overview of industrial wastewater treatments that could be applied in microreactor systems with their advantages over classical processes.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
T1  - Upotreba mikroreaktorskih sistema u procesima prečišćavanja otpadne vode
T1  - The use of microreactor systems in wastewater treatment processes
EP  - 140
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6656
ER  - 

@conference{
author = "Dajić, Ana and Mihajlović, Marina and Svetozarević, Milica",
year = "2022",
abstract = "Porast industrijske aktivnosti za posledicu ima sve veću potrošnju vode a samim tim i sve veće količine otpadne vode opterećene značajnim količinama zagađujućih materija. U vreme kada je zaštita životne sredine aktuelna tema, tehnike prečišćavanja se neprestano unapređuju. Relativno nov koncept prerade otpadne vode uključuje tretmane u mikroreaktorskim sistemima kao atraktivnu alternativu standardnim procesima prečišćavanja. Kako postoji veliki broj različitih tipova mikroreaktorskih sistema i veliki broj metoda uklanjanja zagađujućih materija, otvaraju se nove mogućnosti primene različitih tretmana obrade. U radu je dat pregled tretmana industrijskih otpadnih voda koje je moguće primeniti u mikroreaktorskim sistemima., The increase in industrial activity results in increasing water consumption and increasing amounts of wastewater burdened with significant amounts of pollutants. Environmental protection has become an important topic and wastewater treatment techniques are constantly being improved. A relatively new concept includes treatments in microreactor systems as an attractive alternative to standard processes. Large number of different types of microreactor systems and large number of methods for removing pollutants opens up, new possibilities for the application of different treatment types. The paper gives an overview of industrial wastewater treatments that could be applied in microreactor systems with their advantages over classical processes.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd",
title = "Upotreba mikroreaktorskih sistema u procesima prečišćavanja otpadne vode, The use of microreactor systems in wastewater treatment processes",
pages = "140-137",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6656"
}

Dajić, A., Mihajlović, M.,& Svetozarević, M.. (2022). Upotreba mikroreaktorskih sistema u procesima prečišćavanja otpadne vode. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 137-140.
https://hdl.handle.net/21.15107/rcub_technorep_6656

Dajić A, Mihajlović M, Svetozarević M. Upotreba mikroreaktorskih sistema u procesima prečišćavanja otpadne vode. in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd. 2022;:137-140.
https://hdl.handle.net/21.15107/rcub_technorep_6656 .

Dajić, Ana, Mihajlović, Marina, Svetozarević, Milica, "Upotreba mikroreaktorskih sistema u procesima prečišćavanja otpadne vode" in Zbornik radova / 35. Međunarodni kongres o procesnoj industriji, PROCESING '22, 1-3. jun 2022, Beograd (2022):137-140,
https://hdl.handle.net/21.15107/rcub_technorep_6656 .

TY  - DATA
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Onjia, Antonije
AU  - Barać, Nemanja
AU  - Mihajlović, Marina
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4730
PB  - Elsevier
T2  - Environmental Technology & Innovation
T1  - Supplementary data for article: Svetozarević, M.; Šekuljica, N.; Onjia, A.; Barać, N.; Mihajlović, M.; Knežević-Jugović, Z.; Mijin, D. Biodegradation of Synthetic Dyes by Free and Cross-Linked Peroxidase in Microfluidic Reactor. Environmental Technology & Innovation 2022, 26, 102373. https://doi.org/10.1016/j.eti.2022.102373.
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4730
ER  - 

@misc{
author = "Svetozarević, Milica and Šekuljica, Nataša and Onjia, Antonije and Barać, Nemanja and Mihajlović, Marina and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2022",
publisher = "Elsevier",
journal = "Environmental Technology & Innovation",
title = "Supplementary data for article: Svetozarević, M.; Šekuljica, N.; Onjia, A.; Barać, N.; Mihajlović, M.; Knežević-Jugović, Z.; Mijin, D. Biodegradation of Synthetic Dyes by Free and Cross-Linked Peroxidase in Microfluidic Reactor. Environmental Technology & Innovation 2022, 26, 102373. https://doi.org/10.1016/j.eti.2022.102373.",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4730"
}

Svetozarević, M., Šekuljica, N., Onjia, A., Barać, N., Mihajlović, M., Knežević-Jugović, Z.,& Mijin, D.. (2022). Supplementary data for article: Svetozarević, M.; Šekuljica, N.; Onjia, A.; Barać, N.; Mihajlović, M.; Knežević-Jugović, Z.; Mijin, D. Biodegradation of Synthetic Dyes by Free and Cross-Linked Peroxidase in Microfluidic Reactor. Environmental Technology & Innovation 2022, 26, 102373. https://doi.org/10.1016/j.eti.2022.102373.. in Environmental Technology & Innovation
Elsevier..
https://hdl.handle.net/21.15107/rcub_technorep_4730

Svetozarević M, Šekuljica N, Onjia A, Barać N, Mihajlović M, Knežević-Jugović Z, Mijin D. Supplementary data for article: Svetozarević, M.; Šekuljica, N.; Onjia, A.; Barać, N.; Mihajlović, M.; Knežević-Jugović, Z.; Mijin, D. Biodegradation of Synthetic Dyes by Free and Cross-Linked Peroxidase in Microfluidic Reactor. Environmental Technology & Innovation 2022, 26, 102373. https://doi.org/10.1016/j.eti.2022.102373.. in Environmental Technology & Innovation. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_4730 .

Svetozarević, Milica, Šekuljica, Nataša, Onjia, Antonije, Barać, Nemanja, Mihajlović, Marina, Knežević-Jugović, Zorica, Mijin, Dušan, "Supplementary data for article: Svetozarević, M.; Šekuljica, N.; Onjia, A.; Barać, N.; Mihajlović, M.; Knežević-Jugović, Z.; Mijin, D. Biodegradation of Synthetic Dyes by Free and Cross-Linked Peroxidase in Microfluidic Reactor. Environmental Technology & Innovation 2022, 26, 102373. https://doi.org/10.1016/j.eti.2022.102373." in Environmental Technology & Innovation (2022),
https://hdl.handle.net/21.15107/rcub_technorep_4730 .

TY  - CONF
AU  - Mihajlović, Marina
AU  - Dajić, Ana
AU  - Svetozarević, Milica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5750
AB  - Landfilling is a dominant method of waste management in most developing countries. Moreover, a significant quantity of waste has been disposed on open dumps and unmanaged disposal sites. Landfill gas (LFG) can be considerable source of GHG emissions, as it consists of 50 % methane and 50 % carbon dioxide. Republic of Serbia is a developing country. Its waste management legislation is based on EU legislation. In the first decade of 2000s a set of laws was passed in this area. Unfortunately, the majority of goals were not fulfilled. An updated version of Waste management program is active since 2022. In Serbia, 2.95 million tons of waste is generated every year. It is estimated that 15-20 % is disposed on dumps, while 80 % is collected and disposed on the landfills. Waste treatment does not exist. Recycling rate is a very low, around 15%. Different waste management scenarios of impact on GHG emissions have been analysed. The analysis was performed using the software "Tool for Calculating Greenhouse Gases (GHG) in Solid Waste Management (SWM-GHG calculator)"developed by the German IFEU (ger. Institut für Energie- und Umweltforschung Heidelberg). The base scenario describes the current situation. Scenarios 1 and 2 describe goals set for 2025 and 2030 by Waste management program in the Republic of Serbia for the period 2022 - 2031. Scenario 3 describes best case scenario, with fully functional modern waste management system. The analysis includes several parameters, such as landfill type, recycling rate, LFG collection efficiency and use of waste incineration technology.
PB  - Institute of Physics
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Waste management strategies effects on GHG emission: Case study of Serbia
IS  - 1
SP  - 012074
VL  - 1123
DO  - 10.1088/1755-1315/1123/1/012074
ER  - 

@conference{
author = "Mihajlović, Marina and Dajić, Ana and Svetozarević, Milica",
year = "2022",
abstract = "Landfilling is a dominant method of waste management in most developing countries. Moreover, a significant quantity of waste has been disposed on open dumps and unmanaged disposal sites. Landfill gas (LFG) can be considerable source of GHG emissions, as it consists of 50 % methane and 50 % carbon dioxide. Republic of Serbia is a developing country. Its waste management legislation is based on EU legislation. In the first decade of 2000s a set of laws was passed in this area. Unfortunately, the majority of goals were not fulfilled. An updated version of Waste management program is active since 2022. In Serbia, 2.95 million tons of waste is generated every year. It is estimated that 15-20 % is disposed on dumps, while 80 % is collected and disposed on the landfills. Waste treatment does not exist. Recycling rate is a very low, around 15%. Different waste management scenarios of impact on GHG emissions have been analysed. The analysis was performed using the software "Tool for Calculating Greenhouse Gases (GHG) in Solid Waste Management (SWM-GHG calculator)"developed by the German IFEU (ger. Institut für Energie- und Umweltforschung Heidelberg). The base scenario describes the current situation. Scenarios 1 and 2 describe goals set for 2025 and 2030 by Waste management program in the Republic of Serbia for the period 2022 - 2031. Scenario 3 describes best case scenario, with fully functional modern waste management system. The analysis includes several parameters, such as landfill type, recycling rate, LFG collection efficiency and use of waste incineration technology.",
publisher = "Institute of Physics",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Waste management strategies effects on GHG emission: Case study of Serbia",
number = "1",
pages = "012074",
volume = "1123",
doi = "10.1088/1755-1315/1123/1/012074"
}

Mihajlović, M., Dajić, A.,& Svetozarević, M.. (2022). Waste management strategies effects on GHG emission: Case study of Serbia. in IOP Conference Series: Earth and Environmental Science
Institute of Physics., 1123(1), 012074.
https://doi.org/10.1088/1755-1315/1123/1/012074

Mihajlović M, Dajić A, Svetozarević M. Waste management strategies effects on GHG emission: Case study of Serbia. in IOP Conference Series: Earth and Environmental Science. 2022;1123(1):012074.
doi:10.1088/1755-1315/1123/1/012074 .

Mihajlović, Marina, Dajić, Ana, Svetozarević, Milica, "Waste management strategies effects on GHG emission: Case study of Serbia" in IOP Conference Series: Earth and Environmental Science, 1123, no. 1 (2022):012074,
https://doi.org/10.1088/1755-1315/1123/1/012074 . .

TY  - CONF
AU  - Tadić, Julijana
AU  - Lađarević, Jelena
AU  - Svetozarević, Milica
AU  - Matović, Luka
AU  - Mašulović, Aleksandra
AU  - Mijin, Dušan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6743
AB  - Azo dyes are the most important group of organic synthetic dyes. High molar extinction coefficients, which characterize azo compounds, contribute to their wide use in traditional dyeing of various materials, as well as the increasing application in new technologies such as optical devices and solar cells. Numerous studies show that azo dyes based on heterocyclic molecules possess biological activity. Arylazo pyridone dyes belong to the class of disperse dyes, which exhibit antibacterial, antifungal, anticancer and antioxidant properties. In this work, three azo dyes were synthesized starting from 2-, 3- and 4-aminophenol and 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone. The structure of dyes was confirmed by ATR-FTIR, NMR and UV-Vis spectroscopy. The antioxidant activity of compounds was examined by the ABTS method. Physico-chemical descriptors and ADME parameters were determined in silico using SwissADME program.
AB  - Azo boje su najznačajnija grupa organskih sintetskih boja. Visoki molarni ekstencioni koefici-jenti, koji karakterišu azo jedinjena, doprinose njihovoj širokoj upotrebi kako u tradicionalnom bo-jenju materijala, tako i sve većoj primeni u novijim tehnologijama kao što su optički materijali i solarne ćelije. Mnoga istraživanja pokazuju da azo boje dobijene iz heterocikličnih molekula imaju biološku aktivnost. Arilazo piridonske boje pripadaju klasi disperznih boja koje ispoljavaju antibak-terijsku,  antifungalnu,  antikancerogenu  i  antioksidativnu  aktivnost.  U  ovom  radu  sintetisane  su  tri azo boje polazeći iz 2-, 3-i 4-aminofenola i 3-cijano-6-hidroksi-4-metil-1-propil-2-piridona. Struk-tura jedinjenja potvrđena je ATR-FTIR, NMR i UV-Vis podacima. Antioksidsativna aktivnost jedinje-nja ispitana je ABTS metodom. Fizičko-hemijski deskriptori i ADME parametari određeni su in silico pomoću SwissADME programa.
PB  - Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku
C3  - Zbornik radova / 34. Međunarodni kongres o procesnoj industriji, PROCESING '21, 3. i 4. jun 2021, Novi Sad
T1  - Synthesis and biological evaluation of some azo dyes based on 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone
T1  - Sinteza i biološka svojstva nekih azo boja na bazi 3-cijano-6-hidroksi-4-metil-1-propil-2-piridona
EP  - 88
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6743
ER  - 

@conference{
author = "Tadić, Julijana and Lađarević, Jelena and Svetozarević, Milica and Matović, Luka and Mašulović, Aleksandra and Mijin, Dušan",
year = "2021",
abstract = "Azo dyes are the most important group of organic synthetic dyes. High molar extinction coefficients, which characterize azo compounds, contribute to their wide use in traditional dyeing of various materials, as well as the increasing application in new technologies such as optical devices and solar cells. Numerous studies show that azo dyes based on heterocyclic molecules possess biological activity. Arylazo pyridone dyes belong to the class of disperse dyes, which exhibit antibacterial, antifungal, anticancer and antioxidant properties. In this work, three azo dyes were synthesized starting from 2-, 3- and 4-aminophenol and 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone. The structure of dyes was confirmed by ATR-FTIR, NMR and UV-Vis spectroscopy. The antioxidant activity of compounds was examined by the ABTS method. Physico-chemical descriptors and ADME parameters were determined in silico using SwissADME program., Azo boje su najznačajnija grupa organskih sintetskih boja. Visoki molarni ekstencioni koefici-jenti, koji karakterišu azo jedinjena, doprinose njihovoj širokoj upotrebi kako u tradicionalnom bo-jenju materijala, tako i sve većoj primeni u novijim tehnologijama kao što su optički materijali i solarne ćelije. Mnoga istraživanja pokazuju da azo boje dobijene iz heterocikličnih molekula imaju biološku aktivnost. Arilazo piridonske boje pripadaju klasi disperznih boja koje ispoljavaju antibak-terijsku,  antifungalnu,  antikancerogenu  i  antioksidativnu  aktivnost.  U  ovom  radu  sintetisane  su  tri azo boje polazeći iz 2-, 3-i 4-aminofenola i 3-cijano-6-hidroksi-4-metil-1-propil-2-piridona. Struk-tura jedinjenja potvrđena je ATR-FTIR, NMR i UV-Vis podacima. Antioksidsativna aktivnost jedinje-nja ispitana je ABTS metodom. Fizičko-hemijski deskriptori i ADME parametari određeni su in silico pomoću SwissADME programa.",
publisher = "Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku",
journal = "Zbornik radova / 34. Međunarodni kongres o procesnoj industriji, PROCESING '21, 3. i 4. jun 2021, Novi Sad",
title = "Synthesis and biological evaluation of some azo dyes based on 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone, Sinteza i biološka svojstva nekih azo boja na bazi 3-cijano-6-hidroksi-4-metil-1-propil-2-piridona",
pages = "88-83",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6743"
}

Tadić, J., Lađarević, J., Svetozarević, M., Matović, L., Mašulović, A.,& Mijin, D.. (2021). Synthesis and biological evaluation of some azo dyes based on 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone. in Zbornik radova / 34. Međunarodni kongres o procesnoj industriji, PROCESING '21, 3. i 4. jun 2021, Novi Sad
Beograd : Savez mašinskih i elektrotehničkih inženjera i tehničara Srbije (SMEITS), Društvo za procesnu tehniku., 83-88.
https://hdl.handle.net/21.15107/rcub_technorep_6743

Tadić J, Lađarević J, Svetozarević M, Matović L, Mašulović A, Mijin D. Synthesis and biological evaluation of some azo dyes based on 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone. in Zbornik radova / 34. Međunarodni kongres o procesnoj industriji, PROCESING '21, 3. i 4. jun 2021, Novi Sad. 2021;:83-88.
https://hdl.handle.net/21.15107/rcub_technorep_6743 .

Tadić, Julijana, Lađarević, Jelena, Svetozarević, Milica, Matović, Luka, Mašulović, Aleksandra, Mijin, Dušan, "Synthesis and biological evaluation of some azo dyes based on 3-cyano-6-hydroxy-4-methyl-1-propyl-2-pyridone" in Zbornik radova / 34. Međunarodni kongres o procesnoj industriji, PROCESING '21, 3. i 4. jun 2021, Novi Sad (2021):83-88,
https://hdl.handle.net/21.15107/rcub_technorep_6743 .

TY  - JOUR
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4893
AB  - The number of different technologies available for colored wastewater treatment shows the vastness of the issue. Finding the most feasible one, yet taking into account the cost, the efficiency and eco-friendliness is like searching needle in a haystack. Here, we have taken the eco-friendliness to a level above, using one bio-waste material for treatment of another waste. Potato peel and soybean hull are agroindustrial waste that is at the same time abundant source of potent enzymes such as peroxidases that can be used in oxidation reactions. In this study, the anthraquinone dye Acid Violet 109 is used as a model for simulation of colored wastewater and for oxidation by peroxidase from two bio-waste sources: potato peel and soybean hull. The operational conditions were optimized and a kinetic study was performed in order to obtain detailed information about the behavior of the enzymes when faced with extreme substrate concentration. Under the optimal conditions, 72.78 +/- 3.13% and 66.12 +/- 2.51% biodegradation was achieved with potato peel peroxidase, and soybean hull, respectively. As most of the industrial wastewater treatments require higher temperatures, both soybean hull and potato peel peroxidase were subjected to and proved to be able to operate at significantly higher temperatures up to 70 degrees C.
T2  - Environmental Technology & Innovation
T1  - Agricultural waste as a source of peroxidase for wastewater treatment: Insight in kinetics and process parameters optimization for anthraquinone dye removal
VL  - 21
DO  - 10.1016/j.eti.2020.101289
ER  - 

@article{
author = "Svetozarević, Milica and Šekuljica, Nataša and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2021",
abstract = "The number of different technologies available for colored wastewater treatment shows the vastness of the issue. Finding the most feasible one, yet taking into account the cost, the efficiency and eco-friendliness is like searching needle in a haystack. Here, we have taken the eco-friendliness to a level above, using one bio-waste material for treatment of another waste. Potato peel and soybean hull are agroindustrial waste that is at the same time abundant source of potent enzymes such as peroxidases that can be used in oxidation reactions. In this study, the anthraquinone dye Acid Violet 109 is used as a model for simulation of colored wastewater and for oxidation by peroxidase from two bio-waste sources: potato peel and soybean hull. The operational conditions were optimized and a kinetic study was performed in order to obtain detailed information about the behavior of the enzymes when faced with extreme substrate concentration. Under the optimal conditions, 72.78 +/- 3.13% and 66.12 +/- 2.51% biodegradation was achieved with potato peel peroxidase, and soybean hull, respectively. As most of the industrial wastewater treatments require higher temperatures, both soybean hull and potato peel peroxidase were subjected to and proved to be able to operate at significantly higher temperatures up to 70 degrees C.",
journal = "Environmental Technology & Innovation",
title = "Agricultural waste as a source of peroxidase for wastewater treatment: Insight in kinetics and process parameters optimization for anthraquinone dye removal",
volume = "21",
doi = "10.1016/j.eti.2020.101289"
}

Svetozarević, M., Šekuljica, N., Knežević-Jugović, Z.,& Mijin, D.. (2021). Agricultural waste as a source of peroxidase for wastewater treatment: Insight in kinetics and process parameters optimization for anthraquinone dye removal. in Environmental Technology & Innovation, 21.
https://doi.org/10.1016/j.eti.2020.101289

Svetozarević M, Šekuljica N, Knežević-Jugović Z, Mijin D. Agricultural waste as a source of peroxidase for wastewater treatment: Insight in kinetics and process parameters optimization for anthraquinone dye removal. in Environmental Technology & Innovation. 2021;21.
doi:10.1016/j.eti.2020.101289 .

Svetozarević, Milica, Šekuljica, Nataša, Knežević-Jugović, Zorica, Mijin, Dušan, "Agricultural waste as a source of peroxidase for wastewater treatment: Insight in kinetics and process parameters optimization for anthraquinone dye removal" in Environmental Technology & Innovation, 21 (2021),
https://doi.org/10.1016/j.eti.2020.101289 . .

TY  - JOUR
AU  - Svetozarević, Milica
AU  - Šekuljica, Nataša
AU  - Knežević-Jugović, Zorica
AU  - Mijin, Dušan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4749
AB  - As water contamination emerges as a serious threat to the environment, ventures for cleaner and sustainable solutions are continuously being developed. The present study investigates the ability of crude peroxidase extract from soybean seeds to degrade the anthraquinone dye Acid Violet 109. The influence of the essential parameters pH, dye concentration, hydrogen peroxide dosage, and temperature were inspected. The enzyme had 81.9 % biodegradation at pH 4 in 30 min with 0.1 U peroxidase, 40 mg/l dye concentration, and 1 mM hydrogen peroxide. Considering that substrate concentration can cause reaction inhibition, a kinetic study was performed. Kinetic data fitting using bisubstrate kinetics with a substrate inhibition model revealed the high inhibitory effect of the dye, which was confirmed by the inhibition constant, 7.123.10(-5) mM. Alongside the inhibition constant values, the Ping-Pong Bi-Bi model gave the maximum rates 15.788 and 14.321 mM/min for hydrogen peroxide and dye inhibition, respectively.
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase
EP  - 206
IS  - 2
SP  - 197
VL  - 39
DO  - 10.20450/mjcce.2020.2150
ER  - 

@article{
author = "Svetozarević, Milica and Šekuljica, Nataša and Knežević-Jugović, Zorica and Mijin, Dušan",
year = "2020",
abstract = "As water contamination emerges as a serious threat to the environment, ventures for cleaner and sustainable solutions are continuously being developed. The present study investigates the ability of crude peroxidase extract from soybean seeds to degrade the anthraquinone dye Acid Violet 109. The influence of the essential parameters pH, dye concentration, hydrogen peroxide dosage, and temperature were inspected. The enzyme had 81.9 % biodegradation at pH 4 in 30 min with 0.1 U peroxidase, 40 mg/l dye concentration, and 1 mM hydrogen peroxide. Considering that substrate concentration can cause reaction inhibition, a kinetic study was performed. Kinetic data fitting using bisubstrate kinetics with a substrate inhibition model revealed the high inhibitory effect of the dye, which was confirmed by the inhibition constant, 7.123.10(-5) mM. Alongside the inhibition constant values, the Ping-Pong Bi-Bi model gave the maximum rates 15.788 and 14.321 mM/min for hydrogen peroxide and dye inhibition, respectively.",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase",
pages = "206-197",
number = "2",
volume = "39",
doi = "10.20450/mjcce.2020.2150"
}

Svetozarević, M., Šekuljica, N., Knežević-Jugović, Z.,& Mijin, D.. (2020). Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase. in Macedonian Journal of Chemistry and Chemical Engineering, 39(2), 197-206.
https://doi.org/10.20450/mjcce.2020.2150

Svetozarević M, Šekuljica N, Knežević-Jugović Z, Mijin D. Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase. in Macedonian Journal of Chemistry and Chemical Engineering. 2020;39(2):197-206.
doi:10.20450/mjcce.2020.2150 .

Svetozarević, Milica, Šekuljica, Nataša, Knežević-Jugović, Zorica, Mijin, Dušan, "Optimization and kinetic study of anthraquinone dye removal from colored wastewater using soybean seed as a source of peroxidase" in Macedonian Journal of Chemistry and Chemical Engineering, 39, no. 2 (2020):197-206,
https://doi.org/10.20450/mjcce.2020.2150 . .