TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Kostić, Marina
AU  - Gašić, Uroš
AU  - Prijić, Željana
AU  - Ren, Xiuxia
AU  - Lukić, Milan
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5298
AB  - Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia
IS  - 12
SP  - 1537
VL  - 15
DO  - 10.3390/ph15121537
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Kostić, Marina and Gašić, Uroš and Prijić, Željana and Ren, Xiuxia and Lukić, Milan and Bugarski, Branko",
year = "2022",
abstract = "Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia",
number = "12",
pages = "1537",
volume = "15",
doi = "10.3390/ph15121537"
}

Čutović, N., Marković, T., Kostić, M., Gašić, U., Prijić, Ž., Ren, X., Lukić, M.,& Bugarski, B.. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals
MDPI., 15(12), 1537.
https://doi.org/10.3390/ph15121537

Čutović N, Marković T, Kostić M, Gašić U, Prijić Ž, Ren X, Lukić M, Bugarski B. Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals. 2022;15(12):1537.
doi:10.3390/ph15121537 .

Čutović, Natalija, Marković, Tatjana, Kostić, Marina, Gašić, Uroš, Prijić, Željana, Ren, Xiuxia, Lukić, Milan, Bugarski, Branko, "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia" in Pharmaceuticals, 15, no. 12 (2022):1537,
https://doi.org/10.3390/ph15121537 . .

TY  - JOUR
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Nikolić, Dragan
AU  - Milošević, Nebojša
AU  - Glišić, Ivana
AU  - Marić, Slađana
AU  - Lukić, Milan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4117
AB  - A newly released, late ripening plum cultivar 'Pozna Plava' sets fruit poorly, although it produces high quality fruit. This study aimed to evaluate which factors in the reproductive process could be related to the lack of fruit set. In two consecutive years, establishment of a suitable polleniser and the stage of ovule development at anthesis as well as initial and final fruit set have been studied. In addition to this, the impact made by temperature fluctuations on the interaction between male gametophytes and female sporophytes was also analysed. Growth of the pollen tubes in the style and penetration into the nucellus as well as fruit set were more effective in cross-pollination than in open and self-pollination. A relative delay in ovule development was observed, and most ovules had an embryo sac with eight nuclei. Considering the results of the quantitative parameter study of pollen tube growth in the ovary as well as the results of the stage of ovule development, a conclusion can be made that this cultivar is characterised by an extremely short effective period of pollination.
PB  - Springer, New York
T2  - Erwerbs-Obstbau
T1  - Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set
EP  - 322
IS  - 4
SP  - 313
VL  - 61
DO  - 10.1007/s10341-019-00431-7
ER  - 

@article{
author = "Đorđević, Milena and Cerović, Radosav and Radičević, Sanja and Nikolić, Dragan and Milošević, Nebojša and Glišić, Ivana and Marić, Slađana and Lukić, Milan",
year = "2019",
abstract = "A newly released, late ripening plum cultivar 'Pozna Plava' sets fruit poorly, although it produces high quality fruit. This study aimed to evaluate which factors in the reproductive process could be related to the lack of fruit set. In two consecutive years, establishment of a suitable polleniser and the stage of ovule development at anthesis as well as initial and final fruit set have been studied. In addition to this, the impact made by temperature fluctuations on the interaction between male gametophytes and female sporophytes was also analysed. Growth of the pollen tubes in the style and penetration into the nucellus as well as fruit set were more effective in cross-pollination than in open and self-pollination. A relative delay in ovule development was observed, and most ovules had an embryo sac with eight nuclei. Considering the results of the quantitative parameter study of pollen tube growth in the ovary as well as the results of the stage of ovule development, a conclusion can be made that this cultivar is characterised by an extremely short effective period of pollination.",
publisher = "Springer, New York",
journal = "Erwerbs-Obstbau",
title = "Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set",
pages = "322-313",
number = "4",
volume = "61",
doi = "10.1007/s10341-019-00431-7"
}

Đorđević, M., Cerović, R., Radičević, S., Nikolić, D., Milošević, N., Glišić, I., Marić, S.,& Lukić, M.. (2019). Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set. in Erwerbs-Obstbau
Springer, New York., 61(4), 313-322.
https://doi.org/10.1007/s10341-019-00431-7

Đorđević M, Cerović R, Radičević S, Nikolić D, Milošević N, Glišić I, Marić S, Lukić M. Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set. in Erwerbs-Obstbau. 2019;61(4):313-322.
doi:10.1007/s10341-019-00431-7 .

Đorđević, Milena, Cerović, Radosav, Radičević, Sanja, Nikolić, Dragan, Milošević, Nebojša, Glišić, Ivana, Marić, Slađana, Lukić, Milan, "Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set" in Erwerbs-Obstbau, 61, no. 4 (2019):313-322,
https://doi.org/10.1007/s10341-019-00431-7 . .

TY  - JOUR
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Glišić, Ivana
AU  - Milošević, Nebojša
AU  - Marić, Slađana
AU  - Lukić, Milan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4216
AB  - Factors such as the stage of ovule maturity and longevity at flowering, when the stigma is able to receive the pollen, are significant in terms of fruit set. Various abnormalities during ovule development can often limit pollination, effective pollination period (EPP) and fruit set. In the plum cultivar 'Pozna Plava' (Prunus domestica L.), in the ovaries of open-pollinated flowers and of emasculated unpollinated flowers, we investigated abnormalities in the tissues of primary and secondary ovules during the time of anthesis until 10 days thereafter. At the same time, the viability of the ovules of the unpollinated flowers was examined. Abnormal ovules were considered those with degenerations in the egg apparatus, inverse arrangement of egg cells, more than eight nuclei in the embryo sac as well as degeneration of the nucellus and integuments. In primary ovules, the most frequent abnormality in open-pollinated and unpollinated flowers was the degeneration of the egg apparatus, with frequencies of 14.3-50.0% and 10.0-89.0%, respectively. In the secondary ovules, in both treatments, a completely different picture was obtained. The processes that led to abnormalities in the structure of the secondary ovules first occurred in the cells of the nucellus, while the elements of the embryo sac had a relatively normal cytological organisation. However, the observed abnormalities do not explain the low fruit set of this cultivar.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis
EP  - 228
SP  - 222
VL  - 252
DO  - 10.1016/j.scienta.2019.03.059
ER  - 

@article{
author = "Đorđević, Milena and Cerović, Radosav and Radičević, Sanja and Glišić, Ivana and Milošević, Nebojša and Marić, Slađana and Lukić, Milan",
year = "2019",
abstract = "Factors such as the stage of ovule maturity and longevity at flowering, when the stigma is able to receive the pollen, are significant in terms of fruit set. Various abnormalities during ovule development can often limit pollination, effective pollination period (EPP) and fruit set. In the plum cultivar 'Pozna Plava' (Prunus domestica L.), in the ovaries of open-pollinated flowers and of emasculated unpollinated flowers, we investigated abnormalities in the tissues of primary and secondary ovules during the time of anthesis until 10 days thereafter. At the same time, the viability of the ovules of the unpollinated flowers was examined. Abnormal ovules were considered those with degenerations in the egg apparatus, inverse arrangement of egg cells, more than eight nuclei in the embryo sac as well as degeneration of the nucellus and integuments. In primary ovules, the most frequent abnormality in open-pollinated and unpollinated flowers was the degeneration of the egg apparatus, with frequencies of 14.3-50.0% and 10.0-89.0%, respectively. In the secondary ovules, in both treatments, a completely different picture was obtained. The processes that led to abnormalities in the structure of the secondary ovules first occurred in the cells of the nucellus, while the elements of the embryo sac had a relatively normal cytological organisation. However, the observed abnormalities do not explain the low fruit set of this cultivar.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis",
pages = "228-222",
volume = "252",
doi = "10.1016/j.scienta.2019.03.059"
}

Đorđević, M., Cerović, R., Radičević, S., Glišić, I., Milošević, N., Marić, S.,& Lukić, M.. (2019). Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 252, 222-228.
https://doi.org/10.1016/j.scienta.2019.03.059

Đorđević M, Cerović R, Radičević S, Glišić I, Milošević N, Marić S, Lukić M. Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis. in Scientia Horticulturae. 2019;252:222-228.
doi:10.1016/j.scienta.2019.03.059 .

Đorđević, Milena, Cerović, Radosav, Radičević, Sanja, Glišić, Ivana, Milošević, Nebojša, Marić, Slađana, Lukić, Milan, "Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis" in Scientia Horticulturae, 252 (2019):222-228,
https://doi.org/10.1016/j.scienta.2019.03.059 . .

TY  - JOUR
AU  - Marić, Slađana
AU  - Radičević, Sanja
AU  - Lukić, Milan
AU  - Cerović, Radosav
AU  - Paunović, Svetlana A.
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3572
AB  - Apples and sweet cherries are generally characterized by self-incompatibility, which is controlled by the multiallelic locus S with gametophytic action. Thus the identification of S-genotype represents crucial information for fruit breeders and growers. The aim of this study was to identify the S-genotype of two apple ('Cacanska Pozna' and 'Cadel') and two sweet cherry ('Asenova Rana' and 'Carna') cultivars developed at Fruit Research Institute, Cacak using the polymerase chain reaction (PCR) method with consensus and allele-specific primers. The S-genotype of apple cultivars 'Cacanska Pozna' (S7S9) and 'Cadel' (S2S7) were consistent with parental combinations 'Starking Delicious' (S9S28) x 'Jonathan' (S7S9) and 'Golden Delicious' (S2S3) x 'Jonathan' (S7S9), respectively. For sweet cherry cultivars 'Asenova Rana' ['Drogans Gelbe' (S1S5) x 'Majova Rana' (S1S4)] and 'Carna' ['Majova Rana' (S1S4) x 'Bigarreau de Schrecken' (S1S3)], S3S9 and S1S4 were identified respectively, suggesting that the reported pedigree is erroneous. The trueness-to-type of DNA samples of both sweet cherry cultivars was confirmed by comparison of cultivar samples from more than one site. Upon this testing, the remaining inconsistency indicated that aforementioned parentages of the sweet cherry cultivars were incorrect. These discrepancies could be the result of possible pollen contamination, or mistakes either in breeders recording or in seed processing and seedling production. The results of S-genotyping of apple and sweet cherry cultivars provide relevant information on correct assignment of these cultivars to cross-compatibility groups, which are important for cross design in developing new cultivar, as well as for orchard management in the efficient production of fruits.
PB  - Društvo genetičara Srbije, Beograd
T2  - GENETIKA-BELGRADE
T1  - Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak
EP  - 138
IS  - 1
SP  - 127
VL  - 49
DO  - 10.2298/GENSR1701127M
ER  - 

@article{
author = "Marić, Slađana and Radičević, Sanja and Lukić, Milan and Cerović, Radosav and Paunović, Svetlana A.",
year = "2017",
abstract = "Apples and sweet cherries are generally characterized by self-incompatibility, which is controlled by the multiallelic locus S with gametophytic action. Thus the identification of S-genotype represents crucial information for fruit breeders and growers. The aim of this study was to identify the S-genotype of two apple ('Cacanska Pozna' and 'Cadel') and two sweet cherry ('Asenova Rana' and 'Carna') cultivars developed at Fruit Research Institute, Cacak using the polymerase chain reaction (PCR) method with consensus and allele-specific primers. The S-genotype of apple cultivars 'Cacanska Pozna' (S7S9) and 'Cadel' (S2S7) were consistent with parental combinations 'Starking Delicious' (S9S28) x 'Jonathan' (S7S9) and 'Golden Delicious' (S2S3) x 'Jonathan' (S7S9), respectively. For sweet cherry cultivars 'Asenova Rana' ['Drogans Gelbe' (S1S5) x 'Majova Rana' (S1S4)] and 'Carna' ['Majova Rana' (S1S4) x 'Bigarreau de Schrecken' (S1S3)], S3S9 and S1S4 were identified respectively, suggesting that the reported pedigree is erroneous. The trueness-to-type of DNA samples of both sweet cherry cultivars was confirmed by comparison of cultivar samples from more than one site. Upon this testing, the remaining inconsistency indicated that aforementioned parentages of the sweet cherry cultivars were incorrect. These discrepancies could be the result of possible pollen contamination, or mistakes either in breeders recording or in seed processing and seedling production. The results of S-genotyping of apple and sweet cherry cultivars provide relevant information on correct assignment of these cultivars to cross-compatibility groups, which are important for cross design in developing new cultivar, as well as for orchard management in the efficient production of fruits.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "GENETIKA-BELGRADE",
title = "Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak",
pages = "138-127",
number = "1",
volume = "49",
doi = "10.2298/GENSR1701127M"
}

Marić, S., Radičević, S., Lukić, M., Cerović, R.,& Paunović, S. A.. (2017). Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak. in GENETIKA-BELGRADE
Društvo genetičara Srbije, Beograd., 49(1), 127-138.
https://doi.org/10.2298/GENSR1701127M

Marić S, Radičević S, Lukić M, Cerović R, Paunović SA. Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak. in GENETIKA-BELGRADE. 2017;49(1):127-138.
doi:10.2298/GENSR1701127M .

Marić, Slađana, Radičević, Sanja, Lukić, Milan, Cerović, Radosav, Paunović, Svetlana A., "Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak" in GENETIKA-BELGRADE, 49, no. 1 (2017):127-138,
https://doi.org/10.2298/GENSR1701127M . .