TY  - CHAP
AU  - Grayson, W.L.
AU  - Vunjak-Novaković, Gordana
AU  - Obradović, Bojana
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1978
AB  - The underlying philosophy guiding the design and development of advanced bioreactors for tissue engineering purposes is to provide environmental conditions that enable metabolically active cells in threedimensional (3D) spatial arrangements to reconstruct functional tissues1. This requires that cells receive adequate supplies of nutrients to maintain their viability, along with 'relevant' biological, physiological and mechanical signals to guide their differentiation along a chosen lineagepathway.
T2  - Cell and Tissue Engineering
T1  - Bioreactors in tissue engineering
EP  - 227
SP  - 217
VL  - 9783642219139
DO  - 10.1007/978-3-642-21913-9_11
ER  - 

@inbook{
author = "Grayson, W.L. and Vunjak-Novaković, Gordana and Obradović, Bojana",
year = "2012",
abstract = "The underlying philosophy guiding the design and development of advanced bioreactors for tissue engineering purposes is to provide environmental conditions that enable metabolically active cells in threedimensional (3D) spatial arrangements to reconstruct functional tissues1. This requires that cells receive adequate supplies of nutrients to maintain their viability, along with 'relevant' biological, physiological and mechanical signals to guide their differentiation along a chosen lineagepathway.",
journal = "Cell and Tissue Engineering",
booktitle = "Bioreactors in tissue engineering",
pages = "227-217",
volume = "9783642219139",
doi = "10.1007/978-3-642-21913-9_11"
}

Grayson, W.L., Vunjak-Novaković, G.,& Obradović, B.. (2012). Bioreactors in tissue engineering. in Cell and Tissue Engineering, 9783642219139, 217-227.
https://doi.org/10.1007/978-3-642-21913-9_11

Grayson W, Vunjak-Novaković G, Obradović B. Bioreactors in tissue engineering. in Cell and Tissue Engineering. 2012;9783642219139:217-227.
doi:10.1007/978-3-642-21913-9_11 .

Grayson, W.L., Vunjak-Novaković, Gordana, Obradović, Bojana, "Bioreactors in tissue engineering" in Cell and Tissue Engineering, 9783642219139 (2012):217-227,
https://doi.org/10.1007/978-3-642-21913-9_11 . .

TY  - BOOK
AU  - Balać, Igor
AU  - Bugarski, Branko
AU  - Ćosić, Irena
AU  - Dramićanin, Miroslav
AU  - Đorđević, Drago
AU  - Filipović, Nenad D.
AU  - Ignjatović, Nenad
AU  - Janaćković, Đorđe
AU  - Kojić, Miloš
AU  - Manojlović, Verica
AU  - Marković, Zoran
AU  - Obradović, Bojana
AU  - Pajić-Lijaković, Ivana
AU  - Pavlović, Miodrag
AU  - Plavšić, Milenko B.
AU  - Raković, Dejan
AU  - Ranković, Vladimir
AU  - Stojanović, Boban
AU  - Trajković, Vladimir
AU  - Uskoković, Dragan
AU  - Uskoković, Petar
AU  - Veljković, Dejan
AU  - Vlastelica, Ivo
AU  - Vunjak-Novaković, Gordana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1535
AB  - Početak XXI veka nesumnjivo je obeležen interdisciplinarnim i multidisciplinarnim naporima istraživača u različitim oblastima nauke. Jedna od najizrazitijih tendencija ovog tipa uočava se u biomedicinskim istraživanjima, gde se združuju napori lekara, biologa, genetičara i biohemičara, s jedne strane, i biofizičara i inženjera, s druge strane – sa ciljem dubljeg razumevanja zdravlja i bolesti, i primene ovih saznanja u biomedicinskoj praksi, tako važnoj u svakodnevnom životu ljudi. Kao rezultat ovih svetskih trendova, u Srbiji već više godina na nekoliko fakulteta postoji nastava iz oblasti biomedicinskog inženjerstva, sa ciljem da osposobi inženjere ovih usmerenja za multidisciplinarno povezivanje znanja iz oblasti tehnike sa biomedicinskim znanjima. Jedan od bazičnih predmeta ovih usmerenja jesu Biomaterijali, kojima je i posvećen naš udžbenik, čiji je cilj da predstavi pregled teorije i prakse biomaterijala u biomedicinskoj nauci. Nauka o biomaterijalima je nesumnjivo najmultidisciplinarnija od svih nauka, jer zahteva ovladavanje znanjima iz mnogih oblasti nauke i tehnologije, inženjerstva i medicine, kako bi naučnici iz oblasti biomaterijala mogli da se uhvate u koštac sa ovom profesijom. Zato posle uvodnog dela, udžbenik iz Biomaterijala sadrži četiri celine: (I) Osnovni biomedicinski koncepti i reakcije organizma na biomaterijale, (II) Struktura, fizičko-mehanička karakterizacija i modeliranje biomaterijala i tkiva, (III) Savremeni biomaterijali i tehnologije, (IV) Perspektive biomaterijala i tehnologija, iza kojih slede Zadaci sa rešenjima, Ispitna test pitanja i Ispitna teorijska pitanja, koji pomažu studentima da lakše savladaju veoma obimno i kompleksno gradivo. Na kraju svakog poglavlja data su pitanja za rekapitulaciju, kao i spisak dopunske literature za opcionu detaljniju obradu pojedinih oblasti. Grupa od dvadeset četiri profesionalca sa univerziteta i naučnih instituta, pod okriljem Instituta tehničkih nauka Srpske akademije nauka i umetnosti, Beograd, i Društva za istraživanje materijala Srbije (MRS Srbija) doprinela je pisanju ovog kapitalnog udžbenika o biomaterijalima, prvog do sada na srpskom jeziku. Mada uključivanje veće grupe autora nužno dovodi do stilske neujednačenosti, ipak je oblast biomaterijala toliko multidisciplinarna da je ovakav pristup bio neophodan, kako uostalom pokazuju slična svetska iskustva sa uključivanjem i preko pedeset autora. Ipak urednici su se potrudili da koliko je to moguće stilski i pedagoški ujednače udžbenik, kako bi bio korisna literatura za sve studente diplomskih, master i doktorskih studija iz biomedicinskog inženjerstva u Srbiji i okruženju.
PB  - Beograd : Institut tehničkih nauka Srpske akademije nauka i umetnosti; Društvo za istraživanje materijala
T1  - Biomaterijali
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7344
ER  - 

@book{
author = "Balać, Igor and Bugarski, Branko and Ćosić, Irena and Dramićanin, Miroslav and Đorđević, Drago and Filipović, Nenad D. and Ignjatović, Nenad and Janaćković, Đorđe and Kojić, Miloš and Manojlović, Verica and Marković, Zoran and Obradović, Bojana and Pajić-Lijaković, Ivana and Pavlović, Miodrag and Plavšić, Milenko B. and Raković, Dejan and Ranković, Vladimir and Stojanović, Boban and Trajković, Vladimir and Uskoković, Dragan and Uskoković, Petar and Veljković, Dejan and Vlastelica, Ivo and Vunjak-Novaković, Gordana",
year = "2010",
abstract = "Početak XXI veka nesumnjivo je obeležen interdisciplinarnim i multidisciplinarnim naporima istraživača u različitim oblastima nauke. Jedna od najizrazitijih tendencija ovog tipa uočava se u biomedicinskim istraživanjima, gde se združuju napori lekara, biologa, genetičara i biohemičara, s jedne strane, i biofizičara i inženjera, s druge strane – sa ciljem dubljeg razumevanja zdravlja i bolesti, i primene ovih saznanja u biomedicinskoj praksi, tako važnoj u svakodnevnom životu ljudi. Kao rezultat ovih svetskih trendova, u Srbiji već više godina na nekoliko fakulteta postoji nastava iz oblasti biomedicinskog inženjerstva, sa ciljem da osposobi inženjere ovih usmerenja za multidisciplinarno povezivanje znanja iz oblasti tehnike sa biomedicinskim znanjima. Jedan od bazičnih predmeta ovih usmerenja jesu Biomaterijali, kojima je i posvećen naš udžbenik, čiji je cilj da predstavi pregled teorije i prakse biomaterijala u biomedicinskoj nauci. Nauka o biomaterijalima je nesumnjivo najmultidisciplinarnija od svih nauka, jer zahteva ovladavanje znanjima iz mnogih oblasti nauke i tehnologije, inženjerstva i medicine, kako bi naučnici iz oblasti biomaterijala mogli da se uhvate u koštac sa ovom profesijom. Zato posle uvodnog dela, udžbenik iz Biomaterijala sadrži četiri celine: (I) Osnovni biomedicinski koncepti i reakcije organizma na biomaterijale, (II) Struktura, fizičko-mehanička karakterizacija i modeliranje biomaterijala i tkiva, (III) Savremeni biomaterijali i tehnologije, (IV) Perspektive biomaterijala i tehnologija, iza kojih slede Zadaci sa rešenjima, Ispitna test pitanja i Ispitna teorijska pitanja, koji pomažu studentima da lakše savladaju veoma obimno i kompleksno gradivo. Na kraju svakog poglavlja data su pitanja za rekapitulaciju, kao i spisak dopunske literature za opcionu detaljniju obradu pojedinih oblasti. Grupa od dvadeset četiri profesionalca sa univerziteta i naučnih instituta, pod okriljem Instituta tehničkih nauka Srpske akademije nauka i umetnosti, Beograd, i Društva za istraživanje materijala Srbije (MRS Srbija) doprinela je pisanju ovog kapitalnog udžbenika o biomaterijalima, prvog do sada na srpskom jeziku. Mada uključivanje veće grupe autora nužno dovodi do stilske neujednačenosti, ipak je oblast biomaterijala toliko multidisciplinarna da je ovakav pristup bio neophodan, kako uostalom pokazuju slična svetska iskustva sa uključivanjem i preko pedeset autora. Ipak urednici su se potrudili da koliko je to moguće stilski i pedagoški ujednače udžbenik, kako bi bio korisna literatura za sve studente diplomskih, master i doktorskih studija iz biomedicinskog inženjerstva u Srbiji i okruženju.",
publisher = "Beograd : Institut tehničkih nauka Srpske akademije nauka i umetnosti; Društvo za istraživanje materijala",
title = "Biomaterijali",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7344"
}

Balać, I., Bugarski, B., Ćosić, I., Dramićanin, M., Đorđević, D., Filipović, N. D., Ignjatović, N., Janaćković, Đ., Kojić, M., Manojlović, V., Marković, Z., Obradović, B., Pajić-Lijaković, I., Pavlović, M., Plavšić, M. B., Raković, D., Ranković, V., Stojanović, B., Trajković, V., Uskoković, D., Uskoković, P., Veljković, D., Vlastelica, I.,& Vunjak-Novaković, G.. (2010). Biomaterijali. 
Beograd : Institut tehničkih nauka Srpske akademije nauka i umetnosti; Društvo za istraživanje materijala..
https://hdl.handle.net/21.15107/rcub_vinar_7344

Balać I, Bugarski B, Ćosić I, Dramićanin M, Đorđević D, Filipović ND, Ignjatović N, Janaćković Đ, Kojić M, Manojlović V, Marković Z, Obradović B, Pajić-Lijaković I, Pavlović M, Plavšić MB, Raković D, Ranković V, Stojanović B, Trajković V, Uskoković D, Uskoković P, Veljković D, Vlastelica I, Vunjak-Novaković G. Biomaterijali. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_7344 .

Balać, Igor, Bugarski, Branko, Ćosić, Irena, Dramićanin, Miroslav, Đorđević, Drago, Filipović, Nenad D., Ignjatović, Nenad, Janaćković, Đorđe, Kojić, Miloš, Manojlović, Verica, Marković, Zoran, Obradović, Bojana, Pajić-Lijaković, Ivana, Pavlović, Miodrag, Plavšić, Milenko B., Raković, Dejan, Ranković, Vladimir, Stojanović, Boban, Trajković, Vladimir, Uskoković, Dragan, Uskoković, Petar, Veljković, Dejan, Vlastelica, Ivo, Vunjak-Novaković, Gordana, "Biomaterijali" (2010),
https://hdl.handle.net/21.15107/rcub_vinar_7344 .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Radisić, Milica
AU  - Vunjak-Novaković, Gordana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1542
AB  - Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.
T2  - NATO Science for Peace and Security Series A: Chemistry and Biology
T1  - Biomimetic Approaches to Design of Tissue Engineering Bioreactors
EP  - 129
SP  - 115
DO  - 10.1007/978-90-481-8790-4-7
ER  - 

@article{
author = "Obradović, Bojana and Radisić, Milica and Vunjak-Novaković, Gordana",
year = "2010",
abstract = "Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.",
journal = "NATO Science for Peace and Security Series A: Chemistry and Biology",
title = "Biomimetic Approaches to Design of Tissue Engineering Bioreactors",
pages = "129-115",
doi = "10.1007/978-90-481-8790-4-7"
}

Obradović, B., Radisić, M.,& Vunjak-Novaković, G.. (2010). Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology, 115-129.
https://doi.org/10.1007/978-90-481-8790-4-7

Obradović B, Radisić M, Vunjak-Novaković G. Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology. 2010;:115-129.
doi:10.1007/978-90-481-8790-4-7 .

Obradović, Bojana, Radisić, Milica, Vunjak-Novaković, Gordana, "Biomimetic Approaches to Design of Tissue Engineering Bioreactors" in NATO Science for Peace and Security Series A: Chemistry and Biology (2010):115-129,
https://doi.org/10.1007/978-90-481-8790-4-7 . .

TY  - CHAP
AU  - Freshney, R.I.
AU  - Obradović, Bojana
AU  - Grayson, W.
AU  - Cannizzaro, C.
AU  - Vunjak-Novaković, Gordana
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1025
T2  - Principles of Tissue Engineering
T1  - Principles of tissue culture and bioreactor design
EP  - 183
SP  - 155
DO  - 10.1016/B978-012370615-7/50016-0
ER  - 

@inbook{
author = "Freshney, R.I. and Obradović, Bojana and Grayson, W. and Cannizzaro, C. and Vunjak-Novaković, Gordana",
year = "2007",
journal = "Principles of Tissue Engineering",
booktitle = "Principles of tissue culture and bioreactor design",
pages = "183-155",
doi = "10.1016/B978-012370615-7/50016-0"
}

Freshney, R.I., Obradović, B., Grayson, W., Cannizzaro, C.,& Vunjak-Novaković, G.. (2007). Principles of tissue culture and bioreactor design. in Principles of Tissue Engineering, 155-183.
https://doi.org/10.1016/B978-012370615-7/50016-0

Freshney R, Obradović B, Grayson W, Cannizzaro C, Vunjak-Novaković G. Principles of tissue culture and bioreactor design. in Principles of Tissue Engineering. 2007;:155-183.
doi:10.1016/B978-012370615-7/50016-0 .

Freshney, R.I., Obradović, Bojana, Grayson, W., Cannizzaro, C., Vunjak-Novaković, Gordana, "Principles of tissue culture and bioreactor design" in Principles of Tissue Engineering (2007):155-183,
https://doi.org/10.1016/B978-012370615-7/50016-0 . .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Osmokrović, Andrea
AU  - Bugarski, Branko
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1018
AB  - Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
PB  - 8th Conference of the Yugoslav Materials Research Society
T2  - Materials Science Forum
T1  - Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies
EP  - 422
SP  - 417
VL  - 555
DO  - 10.4028/0-87849-441-3.417
ER  - 

@article{
author = "Obradović, Bojana and Osmokrović, Andrea and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2007",
abstract = "Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.",
publisher = "8th Conference of the Yugoslav Materials Research Society",
journal = "Materials Science Forum",
title = "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies",
pages = "422-417",
volume = "555",
doi = "10.4028/0-87849-441-3.417"
}

Obradović, B., Osmokrović, A., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2007). Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum
8th Conference of the Yugoslav Materials Research Society., 555, 417-422.
https://doi.org/10.4028/0-87849-441-3.417

Obradović B, Osmokrović A, Bugarski B, Bugarski D, Vunjak-Novaković G. Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Materials Science Forum. 2007;555:417-422.
doi:10.4028/0-87849-441-3.417 .

Obradović, Bojana, Osmokrović, Andrea, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies" in Materials Science Forum, 555 (2007):417-422,
https://doi.org/10.4028/0-87849-441-3.417 . .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Radisić, M.
AU  - Vunjak-Novaković, Gordana
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1039
AB  - Efficient transport of oxygen is one of the main requirements in tissue engineering systems in order to avoid cell death in the inner tissue regions and support uniform tissue regeneration. In this paper, we review approaches to design of tissue engineering systems with adequate oxygen delivery for cultivation of cartilage and myocardium, two distinctly different tissue types with respect to the tissue structure and oxygen requirements. Mathematical modeling was used to support experimental results and predict oxygen transport within the cultivated tissues and correlate it to the cell response and tissue properties.
T2  - Fluid Dynamics and Materials Processing
T1  - Oxygen transport in tissue engineering systems: Cartilage and myocardium
EP  - 202
IS  - 3
SP  - 189
VL  - 3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1039
ER  - 

@article{
author = "Obradović, Bojana and Radisić, M. and Vunjak-Novaković, Gordana",
year = "2007",
abstract = "Efficient transport of oxygen is one of the main requirements in tissue engineering systems in order to avoid cell death in the inner tissue regions and support uniform tissue regeneration. In this paper, we review approaches to design of tissue engineering systems with adequate oxygen delivery for cultivation of cartilage and myocardium, two distinctly different tissue types with respect to the tissue structure and oxygen requirements. Mathematical modeling was used to support experimental results and predict oxygen transport within the cultivated tissues and correlate it to the cell response and tissue properties.",
journal = "Fluid Dynamics and Materials Processing",
title = "Oxygen transport in tissue engineering systems: Cartilage and myocardium",
pages = "202-189",
number = "3",
volume = "3",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1039"
}

Obradović, B., Radisić, M.,& Vunjak-Novaković, G.. (2007). Oxygen transport in tissue engineering systems: Cartilage and myocardium. in Fluid Dynamics and Materials Processing, 3(3), 189-202.
https://hdl.handle.net/21.15107/rcub_technorep_1039

Obradović B, Radisić M, Vunjak-Novaković G. Oxygen transport in tissue engineering systems: Cartilage and myocardium. in Fluid Dynamics and Materials Processing. 2007;3(3):189-202.
https://hdl.handle.net/21.15107/rcub_technorep_1039 .

Obradović, Bojana, Radisić, M., Vunjak-Novaković, Gordana, "Oxygen transport in tissue engineering systems: Cartilage and myocardium" in Fluid Dynamics and Materials Processing, 3, no. 3 (2007):189-202,
https://hdl.handle.net/21.15107/rcub_technorep_1039 .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Obradović, Bojana
AU  - Bugarski, Diana
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
PY  - 2006
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/897
AB  - Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate micro beads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological for cartilage and bone (~ 100 m/s) provided convective mass transport through the packed bed while the small bead diameter (~ 500µm) ensured short diffusion distances to the immobilized cells. Over 5 weeks of cultivation, the cells remained viable at a constant density whereas the alginate micro beads retained size and spherical shape. Cell density used in this study ( 5 x 106 cells/ml) was found to be too low to result in cartilage tissue formation. However, in several cases, loosely bonded groups of beads and merged beads without visible boundaries were observed, implying that higher cell densities may lead to development of a continuous extra cellular matrix. This study indicates the potentials of the packed bed bioreactor system in conjunction with alginate micro beads as cell carriers, for precise regulation of the cellular microenvironment in cartilage tissue engineering.
AB  - Efikasan prenos regulatornih molekula je jedan od osnovnih zahteva u kulturama ćelija kostne srži radi podsticaja ćelija na diferencijaciju u željenom pravcu. U ovom radu je razvijen i primenjen bioreaktorski sistem sa pakovanim slojem za kultivaciju ćelija kostne srži miša imobilisanih u alginatne mikročestice proizvedene tehnikom elektrostatičke ekstruzije. Kontinualnim protokom medijuma pri fiziološkim brzinama strujanja (~ 100 µm/s) obezbeđen je konvektivni prenos mase u pakovanom sloju dok su malim prečnikom čestica (~ 500µm) ostvarena mala rastojanja za difuzioni prenos mase do imobilisanih ćelija. U toku 5 nedelja kultivacije, ćelije su ostale vijabilne pri konstantnoj ćelijskoj gustini dok su alginatne mikročestice zadržale veličinu i sferičan oblik. Pokazalo se da je koncentracija ćelija korišćena u ovom radu (5 x 106 cells/ml) suviše niska za formiranje tkiva hrskavice. Međutim, u nekoliko slučajeva nađene su slabo vezane grupe mikročestica, a ponegde i potpuno stopljene mikročestica bez primetnih granica što navodi na pretpostavku da bi pri višim koncentracijama ćelija bio moguć razvoj kontinualnog ekstracelularnog matriksa. Rezultati dobijeni u ovom radu ukazuju na mogućnost primene bioreaktorskog sistema sa pakovanim slojem alginatnih mikročestica, nosača ćelija, za preciznu regulaciju mikro-okoline ćelija u inženjerstvu tkiva hrskavice.
PB  - University of Belgrade - Faculty of Mechanical Engineering, Belgrade
T2  - FME Transactions
T1  - Development of a packed bed bioreactor for cartilage tissue engineering
T1  - Razvoj bioreaktorskog sistema sa pakovanim slojem za primenu u inženjerstvu tkiva hrskavice
EP  - 70
IS  - 2
SP  - 65
VL  - 34
UR  - https://hdl.handle.net/21.15107/rcub_technorep_897
ER  - 

@article{
author = "Osmokrović, Andrea and Obradović, Bojana and Bugarski, Diana and Bugarski, Branko and Vunjak-Novaković, Gordana",
year = "2006",
abstract = "Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate micro beads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological for cartilage and bone (~ 100 m/s) provided convective mass transport through the packed bed while the small bead diameter (~ 500µm) ensured short diffusion distances to the immobilized cells. Over 5 weeks of cultivation, the cells remained viable at a constant density whereas the alginate micro beads retained size and spherical shape. Cell density used in this study ( 5 x 106 cells/ml) was found to be too low to result in cartilage tissue formation. However, in several cases, loosely bonded groups of beads and merged beads without visible boundaries were observed, implying that higher cell densities may lead to development of a continuous extra cellular matrix. This study indicates the potentials of the packed bed bioreactor system in conjunction with alginate micro beads as cell carriers, for precise regulation of the cellular microenvironment in cartilage tissue engineering., Efikasan prenos regulatornih molekula je jedan od osnovnih zahteva u kulturama ćelija kostne srži radi podsticaja ćelija na diferencijaciju u željenom pravcu. U ovom radu je razvijen i primenjen bioreaktorski sistem sa pakovanim slojem za kultivaciju ćelija kostne srži miša imobilisanih u alginatne mikročestice proizvedene tehnikom elektrostatičke ekstruzije. Kontinualnim protokom medijuma pri fiziološkim brzinama strujanja (~ 100 µm/s) obezbeđen je konvektivni prenos mase u pakovanom sloju dok su malim prečnikom čestica (~ 500µm) ostvarena mala rastojanja za difuzioni prenos mase do imobilisanih ćelija. U toku 5 nedelja kultivacije, ćelije su ostale vijabilne pri konstantnoj ćelijskoj gustini dok su alginatne mikročestice zadržale veličinu i sferičan oblik. Pokazalo se da je koncentracija ćelija korišćena u ovom radu (5 x 106 cells/ml) suviše niska za formiranje tkiva hrskavice. Međutim, u nekoliko slučajeva nađene su slabo vezane grupe mikročestica, a ponegde i potpuno stopljene mikročestica bez primetnih granica što navodi na pretpostavku da bi pri višim koncentracijama ćelija bio moguć razvoj kontinualnog ekstracelularnog matriksa. Rezultati dobijeni u ovom radu ukazuju na mogućnost primene bioreaktorskog sistema sa pakovanim slojem alginatnih mikročestica, nosača ćelija, za preciznu regulaciju mikro-okoline ćelija u inženjerstvu tkiva hrskavice.",
publisher = "University of Belgrade - Faculty of Mechanical Engineering, Belgrade",
journal = "FME Transactions",
title = "Development of a packed bed bioreactor for cartilage tissue engineering, Razvoj bioreaktorskog sistema sa pakovanim slojem za primenu u inženjerstvu tkiva hrskavice",
pages = "70-65",
number = "2",
volume = "34",
url = "https://hdl.handle.net/21.15107/rcub_technorep_897"
}

Osmokrović, A., Obradović, B., Bugarski, D., Bugarski, B.,& Vunjak-Novaković, G.. (2006). Development of a packed bed bioreactor for cartilage tissue engineering. in FME Transactions
University of Belgrade - Faculty of Mechanical Engineering, Belgrade., 34(2), 65-70.
https://hdl.handle.net/21.15107/rcub_technorep_897

Osmokrović A, Obradović B, Bugarski D, Bugarski B, Vunjak-Novaković G. Development of a packed bed bioreactor for cartilage tissue engineering. in FME Transactions. 2006;34(2):65-70.
https://hdl.handle.net/21.15107/rcub_technorep_897 .

Osmokrović, Andrea, Obradović, Bojana, Bugarski, Diana, Bugarski, Branko, Vunjak-Novaković, Gordana, "Development of a packed bed bioreactor for cartilage tissue engineering" in FME Transactions, 34, no. 2 (2006):65-70,
https://hdl.handle.net/21.15107/rcub_technorep_897 .

TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Radisić, M
AU  - Obradović, Bojana
PY  - 2006
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/920
AB  - The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick constructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs
EP  - 490
IS  - 4
SP  - 485
VL  - 81
DO  - 10.1002/jctb.1467
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Radisić, M and Obradović, Bojana",
year = "2006",
abstract = "The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick constructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs",
pages = "490-485",
number = "4",
volume = "81",
doi = "10.1002/jctb.1467"
}

Vunjak-Novaković, G., Radisić, M.,& Obradović, B.. (2006). Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 81(4), 485-490.
https://doi.org/10.1002/jctb.1467

Vunjak-Novaković G, Radisić M, Obradović B. Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. in Journal of Chemical Technology and Biotechnology. 2006;81(4):485-490.
doi:10.1002/jctb.1467 .

Vunjak-Novaković, Gordana, Radisić, M, Obradović, Bojana, "Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs" in Journal of Chemical Technology and Biotechnology, 81, no. 4 (2006):485-490,
https://doi.org/10.1002/jctb.1467 . .

TY  - JOUR
AU  - Obradović, B.
AU  - Bugarski, Branko
AU  - Todosijević, Zoran
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2005
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/713
AB  - Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.
PB  - 6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes
T2  - Materials Science Forum
T1  - Mathematical modeling of cell distribution in alginate microbeads
EP  - 536
SP  - 531
VL  - 494
DO  - 10.4028/0-87849-971-7.531
ER  - 

@article{
author = "Obradović, B. and Bugarski, Branko and Todosijević, Zoran and Nedović, Viktor and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2005",
abstract = "Alginate is one of the mostly used hydrogels for cell entrapment aimed for applications in food industry, environmental engineering, pharmacy and biomedicine. One of the major parameters affecting cell viability and activity is cell distribution inside the immobilization matrix. In addition, changes in cell distribution over the cultivation time could indicate mass transfer limitations, favorable local environments or cell differentiation. In this study, immobilization and distribution of brewing yeast in alginate microbeads were investigated as a model system of colony forming cell growth. Cell distributions were attained by image analysis of histological cross-sections of microbeads used in beer fermentation. A mathematical model based on cellular automata approach was developed for three-dimensional simulations of cell arrangement over the fermentation time.",
publisher = "6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes",
journal = "Materials Science Forum",
title = "Mathematical modeling of cell distribution in alginate microbeads",
pages = "536-531",
volume = "494",
doi = "10.4028/0-87849-971-7.531"
}

Obradović, B., Bugarski, B., Todosijević, Z., Nedović, V., Bugarski, D.,& Vunjak-Novaković, G.. (2005). Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum
6th Conference of the Yugoslav Materials Research Society, YUCOMAT VI: Current Research in Advanced Materials and Processes., 494, 531-536.
https://doi.org/10.4028/0-87849-971-7.531

Obradović B, Bugarski B, Todosijević Z, Nedović V, Bugarski D, Vunjak-Novaković G. Mathematical modeling of cell distribution in alginate microbeads. in Materials Science Forum. 2005;494:531-536.
doi:10.4028/0-87849-971-7.531 .

Obradović, B., Bugarski, Branko, Todosijević, Zoran, Nedović, Viktor, Bugarski, Diana, Vunjak-Novaković, Gordana, "Mathematical modeling of cell distribution in alginate microbeads" in Materials Science Forum, 494 (2005):531-536,
https://doi.org/10.4028/0-87849-971-7.531 . .

TY  - CHAP
AU  - Radisić, M.
AU  - Obradović, Bojana
AU  - Vunjak-Novaković, Gordana
PY  - 2005
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/723
AB  - Tissue engineering combines the principles of biology, engineering, and medicine to create functional grafts capable of repairing native tissue following a congenital deformity, disease, or trauma. Engineered tissues can provide high-fidelity models for basic studies of cell function and tissue development, and responses to genetic alterations, drugs, hypoxia, and physical stimuli. The overall objective of tissue engineering is the restoration of normal tissue function. Ideally, lost or damaged tissue should be replaced by an engineered graft that can reestablish appropriate structure, composition, cell signaling, and key function(s) of the native tissue. In light of this paradigm, the clinical utility of tissue engineering will likely depend on our ability to replicate the site-specific properties of the tissue being replaced across different size scales and establish the specific differentiated cell phenotype, the composition, architectural organization, and biomechanical properties of the extracellular matrix (ECM), and provide the continuity and strength of the interface with the neighboring host tissues.
T2  - Scaffolding in Tissue Engineering
T1  - Functional tissue engineering of cartilage and myocardium: Bioreactor aspects
EP  - 530
SP  - 501
UR  - https://hdl.handle.net/21.15107/rcub_technorep_723
ER  - 

@inbook{
author = "Radisić, M. and Obradović, Bojana and Vunjak-Novaković, Gordana",
year = "2005",
abstract = "Tissue engineering combines the principles of biology, engineering, and medicine to create functional grafts capable of repairing native tissue following a congenital deformity, disease, or trauma. Engineered tissues can provide high-fidelity models for basic studies of cell function and tissue development, and responses to genetic alterations, drugs, hypoxia, and physical stimuli. The overall objective of tissue engineering is the restoration of normal tissue function. Ideally, lost or damaged tissue should be replaced by an engineered graft that can reestablish appropriate structure, composition, cell signaling, and key function(s) of the native tissue. In light of this paradigm, the clinical utility of tissue engineering will likely depend on our ability to replicate the site-specific properties of the tissue being replaced across different size scales and establish the specific differentiated cell phenotype, the composition, architectural organization, and biomechanical properties of the extracellular matrix (ECM), and provide the continuity and strength of the interface with the neighboring host tissues.",
journal = "Scaffolding in Tissue Engineering",
booktitle = "Functional tissue engineering of cartilage and myocardium: Bioreactor aspects",
pages = "530-501",
url = "https://hdl.handle.net/21.15107/rcub_technorep_723"
}

Radisić, M., Obradović, B.,& Vunjak-Novaković, G.. (2005). Functional tissue engineering of cartilage and myocardium: Bioreactor aspects. in Scaffolding in Tissue Engineering, 501-530.
https://hdl.handle.net/21.15107/rcub_technorep_723

Radisić M, Obradović B, Vunjak-Novaković G. Functional tissue engineering of cartilage and myocardium: Bioreactor aspects. in Scaffolding in Tissue Engineering. 2005;:501-530.
https://hdl.handle.net/21.15107/rcub_technorep_723 .

Radisić, M., Obradović, Bojana, Vunjak-Novaković, Gordana, "Functional tissue engineering of cartilage and myocardium: Bioreactor aspects" in Scaffolding in Tissue Engineering (2005):501-530,
https://hdl.handle.net/21.15107/rcub_technorep_723 .

TY  - JOUR
AU  - Obradović, B.
AU  - Bugarski, Diana
AU  - Petakov, M
AU  - Jovcić, G
AU  - Stojanović, N
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
PY  - 2004
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/673
AB  - Cartilage tissue engineering based on chondrogenic cells seeded onto biodegradable polymer supports and cultivated in bioreactors can potentially become an effective method for creating functional tissue equivalents. Cultivation in perfused bioreactors can improve the uniformity and structure of the engineered tissues. We report studies of two different supports for immunobilization of mouse bone marrow stromal cells (BMSC) for cultivation in perfused bioreactors: fibrous polyglycolic acid (PGA) scaffolds and alginate microbeads.
PB  - Trans Tech Publications Ltd, Durnten-Zurich
T2  - Progress in Advanced Materials and Processes
T1  - Cell support studies aimed for cartilage tissue engineering in perfused bioreactors
EP  - 553
SP  - 549
VL  - 453-454
DO  - 10.4028/www.scientific.net/MSF.453-454.549
ER  - 

@article{
author = "Obradović, B. and Bugarski, Diana and Petakov, M and Jovcić, G and Stojanović, N and Bugarski, Branko and Vunjak-Novaković, Gordana",
year = "2004",
abstract = "Cartilage tissue engineering based on chondrogenic cells seeded onto biodegradable polymer supports and cultivated in bioreactors can potentially become an effective method for creating functional tissue equivalents. Cultivation in perfused bioreactors can improve the uniformity and structure of the engineered tissues. We report studies of two different supports for immunobilization of mouse bone marrow stromal cells (BMSC) for cultivation in perfused bioreactors: fibrous polyglycolic acid (PGA) scaffolds and alginate microbeads.",
publisher = "Trans Tech Publications Ltd, Durnten-Zurich",
journal = "Progress in Advanced Materials and Processes",
title = "Cell support studies aimed for cartilage tissue engineering in perfused bioreactors",
pages = "553-549",
volume = "453-454",
doi = "10.4028/www.scientific.net/MSF.453-454.549"
}

Obradović, B., Bugarski, D., Petakov, M., Jovcić, G., Stojanović, N., Bugarski, B.,& Vunjak-Novaković, G.. (2004). Cell support studies aimed for cartilage tissue engineering in perfused bioreactors. in Progress in Advanced Materials and Processes
Trans Tech Publications Ltd, Durnten-Zurich., 453-454, 549-553.
https://doi.org/10.4028/www.scientific.net/MSF.453-454.549

Obradović B, Bugarski D, Petakov M, Jovcić G, Stojanović N, Bugarski B, Vunjak-Novaković G. Cell support studies aimed for cartilage tissue engineering in perfused bioreactors. in Progress in Advanced Materials and Processes. 2004;453-454:549-553.
doi:10.4028/www.scientific.net/MSF.453-454.549 .

Obradović, B., Bugarski, Diana, Petakov, M, Jovcić, G, Stojanović, N, Bugarski, Branko, Vunjak-Novaković, Gordana, "Cell support studies aimed for cartilage tissue engineering in perfused bioreactors" in Progress in Advanced Materials and Processes, 453-454 (2004):549-553,
https://doi.org/10.4028/www.scientific.net/MSF.453-454.549 . .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Bugarski, Branko
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2004
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/637
AB  - In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue.
AB  - U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.
PB  - Institut bezbednosti, Beograd
T2  - Nauka, tehnika, bezbednost
T1  - In vitro tissue cultures: Microencapsulation and bioreactor cultivation
T1  - In vitro kulture tkiva - mikroenkapsulacija i bioreaktorska kultivacija
EP  - 68
IS  - 2
SP  - 59
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_technorep_637
ER  - 

@article{
author = "Obradović, Bojana and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2004",
abstract = "In this paper, two strategies for in vitro tissue cultivation are presented Microencapsulation is aimed for transplantation of functional tissues encapsulated in semi-permeable membranes, which provide efficient transport of nutrients, gases and small molecules necessary for tissue survival and normal function, and, in the same time, tissue protection from immunological response. Pancreatic islets were encapsulated in poly-L-ornitine - alginate microcapsules by electrostatic droplet generation technique for potential treatment of diabetes. Another approach to tissue cultivation is in vitro regeneration of functional tissue equivalents based on autologous cell biodegradable polymer scaffolds and bioreactor cultivation. Under optimal in vitro conditions (three-dimensional, biodegradable polymer scaffolds dynamic laminar flow in rotating bioreactors) biochemical composition morphology and biomechanical properties of engineered cartilage tissue approached those of the native tissue., U ovom radu su prikazana dva pravca razvoja kultura tkiva za potencijalnu primenu u medicini. Mikroenkapsulacija je usmerena na transplantaciju funkcionalnih tkiva zaštićenih polupropustljivom membranom koja treba da obezbedi efikasnu razmenu materija neophodnih za održavanje viabilnosti i funkcije tkiva, i istovremenu zaštitu od imunološkog odgovora organizma. Kao model sistem, ćelije pankreasa su enkapsulirane primenom tehnike elektrostatičke ekstruzije u mikrokapsule od alginata i poli-L-ornitina za potencijalnu primenu u lečenju dijabetesa. Drugi pravac istraživanja predstavlja in vitro kultivaciju funkcionalnih tkiva baziranu na autolognim ćelijama, biodegradabilnim polimernim nosačima i bioreaktorskoj kultivaciji. Optimizacijom karakteristika polimernih nosača ćelija, uslova kultivacije tipa i režima rada bioreaktora dobijeno je kultivisano tkivo hrskavice koje se po biohemijskim, strukturnim, morfološkim i biomehaničkim karakteristikama približava prirodnom tkivu.",
publisher = "Institut bezbednosti, Beograd",
journal = "Nauka, tehnika, bezbednost",
title = "In vitro tissue cultures: Microencapsulation and bioreactor cultivation, In vitro kulture tkiva - mikroenkapsulacija i bioreaktorska kultivacija",
pages = "68-59",
number = "2",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_technorep_637"
}

Obradović, B., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2004). In vitro tissue cultures: Microencapsulation and bioreactor cultivation. in Nauka, tehnika, bezbednost
Institut bezbednosti, Beograd., 14(2), 59-68.
https://hdl.handle.net/21.15107/rcub_technorep_637

Obradović B, Bugarski B, Bugarski D, Vunjak-Novaković G. In vitro tissue cultures: Microencapsulation and bioreactor cultivation. in Nauka, tehnika, bezbednost. 2004;14(2):59-68.
https://hdl.handle.net/21.15107/rcub_technorep_637 .

Obradović, Bojana, Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "In vitro tissue cultures: Microencapsulation and bioreactor cultivation" in Nauka, tehnika, bezbednost, 14, no. 2 (2004):59-68,
https://hdl.handle.net/21.15107/rcub_technorep_637 .

TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Radišić, Milica
AU  - Obradović, Bojana
PY  - 2004
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/654
AB  - The restoration of normal cardiac function can potentially be achieved by functional tissue constructs grown in vitro by using differentiated or progenitor cells, biomaterial scaffolds and bioreactors. Engineered tissues can also serve as high-fidelity models for basic studies of cells and tissues, in response to gene alterations, drugs, hypoxia, or physical stimuli.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Cardiac tissue engineering
EP  - 67
IS  - 6a
SP  - 65
VL  - 58
UR  - https://hdl.handle.net/21.15107/rcub_technorep_654
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Radišić, Milica and Obradović, Bojana",
year = "2004",
abstract = "The restoration of normal cardiac function can potentially be achieved by functional tissue constructs grown in vitro by using differentiated or progenitor cells, biomaterial scaffolds and bioreactors. Engineered tissues can also serve as high-fidelity models for basic studies of cells and tissues, in response to gene alterations, drugs, hypoxia, or physical stimuli.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Cardiac tissue engineering",
pages = "67-65",
number = "6a",
volume = "58",
url = "https://hdl.handle.net/21.15107/rcub_technorep_654"
}

Vunjak-Novaković, G., Radišić, M.,& Obradović, B.. (2004). Cardiac tissue engineering. in Hemijska industrija
Association of Chemical Engineers of Serbia., 58(6a), 65-67.
https://hdl.handle.net/21.15107/rcub_technorep_654

Vunjak-Novaković G, Radišić M, Obradović B. Cardiac tissue engineering. in Hemijska industrija. 2004;58(6a):65-67.
https://hdl.handle.net/21.15107/rcub_technorep_654 .

Vunjak-Novaković, Gordana, Radišić, Milica, Obradović, Bojana, "Cardiac tissue engineering" in Hemijska industrija, 58, no. 6a (2004):65-67,
https://hdl.handle.net/21.15107/rcub_technorep_654 .

TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Obradović, Bojana
AU  - Martin, Ivan
AU  - Freed, LE
PY  - 2002
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/445
AB  - Functional tissue engineering of cartilage involves the use of bioreactors designed to provide a controlled in vitro environment that embodies some of the biochemical and physical signals known to regulate chondrogenesis. Hydrodynamic conditions can affect in vitro tissue formation in at least two ways: by direct effects of hydrodynamic forces on cell morphology and function, and by indirect flow-induced changes in mass transfer of nutrients and metabolites. In the present work, we discuss the effects of three different in vitro environments: static flasks (tissues fixed in place, static medium), mixed flasks (tissues fixed in place, unidirectional turbulent flow) and rotating bioreactors (tissues dynamically suspended in laminar flow) on engineered cartilage constructs and native cartilage explants. As compared to static and mixed flasks, dynamic laminar flow in rotating bioreactors resulted in the most rapid tissue growth and the highest final fractions of glycosaminoglycans and total collagen in both tissues. Mechanical properties (equilibrium modulus, dynamic stiffness, hydraulic permeability) of engineered constructs and explanted cartilage correlated with the wet weight fractions of glycosaminoglycans and collagen. Current research needs in the area of cartilage tissue engineering include the utilization of additional physiologically relevant regulatory signals, and the development of predictive mathematical models that enable optimization of the conditions and duration of tissue culture.
PB  - IOS Press, Amsterdam
T2  - Biorheology
T1  - Bioreactor studies of native and tissue engineered cartilage
EP  - 268
IS  - 1-2
SP  - 259
VL  - 39
UR  - https://hdl.handle.net/21.15107/rcub_technorep_445
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Obradović, Bojana and Martin, Ivan and Freed, LE",
year = "2002",
abstract = "Functional tissue engineering of cartilage involves the use of bioreactors designed to provide a controlled in vitro environment that embodies some of the biochemical and physical signals known to regulate chondrogenesis. Hydrodynamic conditions can affect in vitro tissue formation in at least two ways: by direct effects of hydrodynamic forces on cell morphology and function, and by indirect flow-induced changes in mass transfer of nutrients and metabolites. In the present work, we discuss the effects of three different in vitro environments: static flasks (tissues fixed in place, static medium), mixed flasks (tissues fixed in place, unidirectional turbulent flow) and rotating bioreactors (tissues dynamically suspended in laminar flow) on engineered cartilage constructs and native cartilage explants. As compared to static and mixed flasks, dynamic laminar flow in rotating bioreactors resulted in the most rapid tissue growth and the highest final fractions of glycosaminoglycans and total collagen in both tissues. Mechanical properties (equilibrium modulus, dynamic stiffness, hydraulic permeability) of engineered constructs and explanted cartilage correlated with the wet weight fractions of glycosaminoglycans and collagen. Current research needs in the area of cartilage tissue engineering include the utilization of additional physiologically relevant regulatory signals, and the development of predictive mathematical models that enable optimization of the conditions and duration of tissue culture.",
publisher = "IOS Press, Amsterdam",
journal = "Biorheology",
title = "Bioreactor studies of native and tissue engineered cartilage",
pages = "268-259",
number = "1-2",
volume = "39",
url = "https://hdl.handle.net/21.15107/rcub_technorep_445"
}

Vunjak-Novaković, G., Obradović, B., Martin, I.,& Freed, L.. (2002). Bioreactor studies of native and tissue engineered cartilage. in Biorheology
IOS Press, Amsterdam., 39(1-2), 259-268.
https://hdl.handle.net/21.15107/rcub_technorep_445

Vunjak-Novaković G, Obradović B, Martin I, Freed L. Bioreactor studies of native and tissue engineered cartilage. in Biorheology. 2002;39(1-2):259-268.
https://hdl.handle.net/21.15107/rcub_technorep_445 .

Vunjak-Novaković, Gordana, Obradović, Bojana, Martin, Ivan, Freed, LE, "Bioreactor studies of native and tissue engineered cartilage" in Biorheology, 39, no. 1-2 (2002):259-268,
https://hdl.handle.net/21.15107/rcub_technorep_445 .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Martin, Ivan
AU  - Padera, RF
AU  - Treppo, S
AU  - Freed, LE
AU  - Vunjak-Novaković, Gordana
PY  - 2001
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/371
AB  - The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Orthopaedic Research
T1  - Integration of engineered cartilage
EP  - 1097
IS  - 6
SP  - 1089
VL  - 19
DO  - 10.1016/S0736-0266(01)00030-4
ER  - 

@article{
author = "Obradović, Bojana and Martin, Ivan and Padera, RF and Treppo, S and Freed, LE and Vunjak-Novaković, Gordana",
year = "2001",
abstract = "The structure and function of cartilaginous constructs, engineered in vitro using bovine articular chondrocytes, biodegradable scaffolds and bioreactors, can be modulated by the conditions and duration of tissue cultivation. We hypothesized that the integrative properties of engineered cartilage depend on developmental stage of the construct and the extracellular matrix content of adjacent cartilage, and that some aspects of integration can be studied under controlled in vitro conditions. Disc-shaped constructs (cultured for 5 +/- 1 days or 5 +/- 1 weeks) or explants (untreated or trypsin treated cartilage) were sutured into ring-shaped explants (untreated or trypsin treated cartilage) to form composites that were cultured for an additional 1-8 weeks in bioreactors and evaluated biochemically, histologically and mechanically (compressive stiffness of the central disk, adhesive strength of the integration interface). Immature constructs had poorer mechanical properties but integrated better than either more mature constructs or cartilage explants. Integration of immature constructs involved cell proliferation and the progressive formation of cartilaginous tissue, in contrast to the integration of more mature constructs or native cartilage which involved only the secretion of extracellular matrix components. Integration patterns correlated with the adhesive strength of the disc-ring interface, which was markedly higher for immature constructs than for either more mature constructs or cartilage explants. Trypsin treatment of the adjacent cartilage further enhanced the integration of immature constructs.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Orthopaedic Research",
title = "Integration of engineered cartilage",
pages = "1097-1089",
number = "6",
volume = "19",
doi = "10.1016/S0736-0266(01)00030-4"
}

Obradović, B., Martin, I., Padera, R., Treppo, S., Freed, L.,& Vunjak-Novaković, G.. (2001). Integration of engineered cartilage. in Journal of Orthopaedic Research
Elsevier Sci Ltd, Oxford., 19(6), 1089-1097.
https://doi.org/10.1016/S0736-0266(01)00030-4

Obradović B, Martin I, Padera R, Treppo S, Freed L, Vunjak-Novaković G. Integration of engineered cartilage. in Journal of Orthopaedic Research. 2001;19(6):1089-1097.
doi:10.1016/S0736-0266(01)00030-4 .

Obradović, Bojana, Martin, Ivan, Padera, RF, Treppo, S, Freed, LE, Vunjak-Novaković, Gordana, "Integration of engineered cartilage" in Journal of Orthopaedic Research, 19, no. 6 (2001):1089-1097,
https://doi.org/10.1016/S0736-0266(01)00030-4 . .

TY  - JOUR
AU  - Plavšić, Milenko B.
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
AU  - Uskoković, Dragan
AU  - Goosen, M.F.
AU  - Jovanović, G.N.
AU  - Ignjatović, Nenad
AU  - Tomić, Simonida
AU  - Pajić-Lijaković, Ivana
PY  - 2000
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/316
AB  - The influence of the molecular weight of poly (L-lactide) on the properties important for biomedical application are reviewed. The utilization of polylactides in sutures, drug release systems and bone reconstruction is analyzed, the considering interrelations between their biocompatibility, biodegradability, good mechanical properties and molecular weight, chain stiffness and crystallinity. The results for polylactides having average molecular weight in the range of 20x103-500x103 g/mol are presented, indicating the different optimal values, depending on the usage.
AB  - Dat je pregled rezultata istraživanja uticaja molske mase poli (L-laktida) na svojstva značajna za njegovu primenu u medicini. Analizirano je korišćenje polilaktida pri ušivanju tkiva, kontrolisanom otpuštanju lekova i lečenju preloma kostiju, razmatrajući povezanost njegovih mehaničkih svojstava, biokompatibilnosti i biodegradabilnosti sa molskom masom, krutošću lanca i kristalnošću. Prikazani su rezultati za polilaktid srednje molske kase u opsegu od 20x103-500x103 g/mol i ukazano na javljanje različitih vrednosti kao optimalnih, zavisno od namene materijala.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - The significance of molecular weight for medical purpose application of PLLA
T1  - Značaj molske mase za primenu PLLA u medicini
EP  - 457
IS  - 10
SP  - 447
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_technorep_316
ER  - 

@article{
author = "Plavšić, Milenko B. and Bugarski, Branko and Vunjak-Novaković, Gordana and Uskoković, Dragan and Goosen, M.F. and Jovanović, G.N. and Ignjatović, Nenad and Tomić, Simonida and Pajić-Lijaković, Ivana",
year = "2000",
abstract = "The influence of the molecular weight of poly (L-lactide) on the properties important for biomedical application are reviewed. The utilization of polylactides in sutures, drug release systems and bone reconstruction is analyzed, the considering interrelations between their biocompatibility, biodegradability, good mechanical properties and molecular weight, chain stiffness and crystallinity. The results for polylactides having average molecular weight in the range of 20x103-500x103 g/mol are presented, indicating the different optimal values, depending on the usage., Dat je pregled rezultata istraživanja uticaja molske mase poli (L-laktida) na svojstva značajna za njegovu primenu u medicini. Analizirano je korišćenje polilaktida pri ušivanju tkiva, kontrolisanom otpuštanju lekova i lečenju preloma kostiju, razmatrajući povezanost njegovih mehaničkih svojstava, biokompatibilnosti i biodegradabilnosti sa molskom masom, krutošću lanca i kristalnošću. Prikazani su rezultati za polilaktid srednje molske kase u opsegu od 20x103-500x103 g/mol i ukazano na javljanje različitih vrednosti kao optimalnih, zavisno od namene materijala.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "The significance of molecular weight for medical purpose application of PLLA, Značaj molske mase za primenu PLLA u medicini",
pages = "457-447",
number = "10",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_technorep_316"
}

Plavšić, M. B., Bugarski, B., Vunjak-Novaković, G., Uskoković, D., Goosen, M.F., Jovanović, G.N., Ignjatović, N., Tomić, S.,& Pajić-Lijaković, I.. (2000). The significance of molecular weight for medical purpose application of PLLA. in Hemijska industrija
Association of Chemical Engineers of Serbia., 54(10), 447-457.
https://hdl.handle.net/21.15107/rcub_technorep_316

Plavšić MB, Bugarski B, Vunjak-Novaković G, Uskoković D, Goosen M, Jovanović G, Ignjatović N, Tomić S, Pajić-Lijaković I. The significance of molecular weight for medical purpose application of PLLA. in Hemijska industrija. 2000;54(10):447-457.
https://hdl.handle.net/21.15107/rcub_technorep_316 .

Plavšić, Milenko B., Bugarski, Branko, Vunjak-Novaković, Gordana, Uskoković, Dragan, Goosen, M.F., Jovanović, G.N., Ignjatović, Nenad, Tomić, Simonida, Pajić-Lijaković, Ivana, "The significance of molecular weight for medical purpose application of PLLA" in Hemijska industrija, 54, no. 10 (2000):447-457,
https://hdl.handle.net/21.15107/rcub_technorep_316 .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Meldon, JH
AU  - Freed, LE
AU  - Vunjak-Novaković, Gordana
PY  - 2000
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/302
AB  - Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.
PB  - Wiley-Blackwell, Malden
T2  - AICHE Journal
T1  - Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model
EP  - 1871
IS  - 9
SP  - 1860
VL  - 46
DO  - 10.1002/aic.690460914
ER  - 

@article{
author = "Obradović, Bojana and Meldon, JH and Freed, LE and Vunjak-Novaković, Gordana",
year = "2000",
abstract = "Functional cartilaginous constructs for scientific research and eventual tissue repair were cultivated in bioreactors starting from chondrocytes immobilized on polymeric scaffolds. The scaffolds gradually degraded as the cells regenerated tissue matrix consisting of glycosaminoglycan (GAG) and type II collagen. To facilitate data interpretation and optimize cultivation conditions, a mathematical model was developed which yields the concentrations of oxygen and GAG as functions of time and position in growing tissue. Calculated GAG concentrations were qualitatively and quantitatively consistent with profiles measured via high-resolution image processing of tissue samples cultured at two different oxygen tensions for various periods of time.",
publisher = "Wiley-Blackwell, Malden",
journal = "AICHE Journal",
title = "Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model",
pages = "1871-1860",
number = "9",
volume = "46",
doi = "10.1002/aic.690460914"
}

Obradović, B., Meldon, J., Freed, L.,& Vunjak-Novaković, G.. (2000). Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. in AICHE Journal
Wiley-Blackwell, Malden., 46(9), 1860-1871.
https://doi.org/10.1002/aic.690460914

Obradović B, Meldon J, Freed L, Vunjak-Novaković G. Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model. in AICHE Journal. 2000;46(9):1860-1871.
doi:10.1002/aic.690460914 .

Obradović, Bojana, Meldon, JH, Freed, LE, Vunjak-Novaković, Gordana, "Glycosaminoglycan deposition in engineered cartilage: Experiments and mathematical model" in AICHE Journal, 46, no. 9 (2000):1860-1871,
https://doi.org/10.1002/aic.690460914 . .

TY  - JOUR
AU  - Martin, Ivan
AU  - Obradović, Bojana
AU  - Treppo, S
AU  - Grodzinsky, AJ
AU  - Langer, R
AU  - Freed, LE
AU  - Vunjak-Novaković, Gordana
PY  - 2000
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/298
AB  - Cartilaginous constructs have been grown in vitro using chondrocytes, biodegradable polymer scaffolds, and tissue culture bioreactors. In the present work, we studied how the composition and mechanical properties of engineered cartilage can be modulated by the conditions and duration of in vitro cultivation, using three different environments: static flasks, mixed flasks, and rotating vessels. After 4-6 weeks, static culture yielded small and fragile constructs, while turbulent flow in mixed flasks induced the formation of an outer fibrous capsule; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels had the highest fractions of glycosaminoglycans and collagen (respectively 75% and 39% of levels measured in native cartilage), and the best mechanical properties (equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential were all about 20% of values measured in native cartilage). Chondrocytes in cartilaginous constructs remained metabolically active and phenotypically stable over prolonged cultivation in rotating bioreactors. The wet weight fraction of glycosaminoglycans and equilibrium modulus of 7 month constructs reached or exceeded the corresponding values measured from freshly explanted native cartilage. Taken together, these findings suggest that functional equivalents of native cartilage can be engineered by optimizing the hydrodynamic conditions in tissue culture bioreactors and the duration of tissue cultivation.
PB  - IOS Press, Amsterdam
T2  - Biorheology
T1  - Modulation of the mechanical properties of tissue engineered cartilage
EP  - 147
IS  - 1-2
SP  - 141
VL  - 37
UR  - https://hdl.handle.net/21.15107/rcub_technorep_298
ER  - 

@article{
author = "Martin, Ivan and Obradović, Bojana and Treppo, S and Grodzinsky, AJ and Langer, R and Freed, LE and Vunjak-Novaković, Gordana",
year = "2000",
abstract = "Cartilaginous constructs have been grown in vitro using chondrocytes, biodegradable polymer scaffolds, and tissue culture bioreactors. In the present work, we studied how the composition and mechanical properties of engineered cartilage can be modulated by the conditions and duration of in vitro cultivation, using three different environments: static flasks, mixed flasks, and rotating vessels. After 4-6 weeks, static culture yielded small and fragile constructs, while turbulent flow in mixed flasks induced the formation of an outer fibrous capsule; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels had the highest fractions of glycosaminoglycans and collagen (respectively 75% and 39% of levels measured in native cartilage), and the best mechanical properties (equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential were all about 20% of values measured in native cartilage). Chondrocytes in cartilaginous constructs remained metabolically active and phenotypically stable over prolonged cultivation in rotating bioreactors. The wet weight fraction of glycosaminoglycans and equilibrium modulus of 7 month constructs reached or exceeded the corresponding values measured from freshly explanted native cartilage. Taken together, these findings suggest that functional equivalents of native cartilage can be engineered by optimizing the hydrodynamic conditions in tissue culture bioreactors and the duration of tissue cultivation.",
publisher = "IOS Press, Amsterdam",
journal = "Biorheology",
title = "Modulation of the mechanical properties of tissue engineered cartilage",
pages = "147-141",
number = "1-2",
volume = "37",
url = "https://hdl.handle.net/21.15107/rcub_technorep_298"
}

Martin, I., Obradović, B., Treppo, S., Grodzinsky, A., Langer, R., Freed, L.,& Vunjak-Novaković, G.. (2000). Modulation of the mechanical properties of tissue engineered cartilage. in Biorheology
IOS Press, Amsterdam., 37(1-2), 141-147.
https://hdl.handle.net/21.15107/rcub_technorep_298

Martin I, Obradović B, Treppo S, Grodzinsky A, Langer R, Freed L, Vunjak-Novaković G. Modulation of the mechanical properties of tissue engineered cartilage. in Biorheology. 2000;37(1-2):141-147.
https://hdl.handle.net/21.15107/rcub_technorep_298 .

Martin, Ivan, Obradović, Bojana, Treppo, S, Grodzinsky, AJ, Langer, R, Freed, LE, Vunjak-Novaković, Gordana, "Modulation of the mechanical properties of tissue engineered cartilage" in Biorheology, 37, no. 1-2 (2000):141-147,
https://hdl.handle.net/21.15107/rcub_technorep_298 .

TY  - JOUR
AU  - Martin, Ivan
AU  - Obradović, Bojana
AU  - Freed, LE
AU  - Vunjak-Novaković, Gordana
PY  - 1999
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/252
AB  - Cartilage tissue engineering can provide a valuable tool for controlled studies of tissue development. As an example, analysis of the spatial distribution of glycosaminoglycans (GAG) in sections of cartilaginous tissues engineered under different culture conditions could be used to correlate the effects of environmental factors with the structure of the regenerated tissue. In this paper we describe a computer-based technique for quantitative analysis of safranin-O stained histological sections, using low magnification light microscopy images. We identified a parameter to quantify the intensity of red color in the sections, which in turn was proportional to the biochemically determined wet weight fraction of GAG in corresponding tissue samples, and to describe the spatial distribution of GAG as a function of depth from. the section edge. A broken line regression model was then used to determine the thickness of an external region, with lower GAG fractions, and the spatial rate of change in GAG content. The method was applied to the quantitatation of GAG distribution in samples of natural and engineered cartilage, cultured for 6 weeks in three different vessels: static flasks, mixed flasks, and rotating bioreactors.
PB  - Amer Inst Physics, Woodbury
T2  - Annals of Biomedical Engineering
T1  - Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage
EP  - 662
IS  - 5
SP  - 656
VL  - 27
DO  - 10.1114/1.205
ER  - 

@article{
author = "Martin, Ivan and Obradović, Bojana and Freed, LE and Vunjak-Novaković, Gordana",
year = "1999",
abstract = "Cartilage tissue engineering can provide a valuable tool for controlled studies of tissue development. As an example, analysis of the spatial distribution of glycosaminoglycans (GAG) in sections of cartilaginous tissues engineered under different culture conditions could be used to correlate the effects of environmental factors with the structure of the regenerated tissue. In this paper we describe a computer-based technique for quantitative analysis of safranin-O stained histological sections, using low magnification light microscopy images. We identified a parameter to quantify the intensity of red color in the sections, which in turn was proportional to the biochemically determined wet weight fraction of GAG in corresponding tissue samples, and to describe the spatial distribution of GAG as a function of depth from. the section edge. A broken line regression model was then used to determine the thickness of an external region, with lower GAG fractions, and the spatial rate of change in GAG content. The method was applied to the quantitatation of GAG distribution in samples of natural and engineered cartilage, cultured for 6 weeks in three different vessels: static flasks, mixed flasks, and rotating bioreactors.",
publisher = "Amer Inst Physics, Woodbury",
journal = "Annals of Biomedical Engineering",
title = "Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage",
pages = "662-656",
number = "5",
volume = "27",
doi = "10.1114/1.205"
}

Martin, I., Obradović, B., Freed, L.,& Vunjak-Novaković, G.. (1999). Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage. in Annals of Biomedical Engineering
Amer Inst Physics, Woodbury., 27(5), 656-662.
https://doi.org/10.1114/1.205

Martin I, Obradović B, Freed L, Vunjak-Novaković G. Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage. in Annals of Biomedical Engineering. 1999;27(5):656-662.
doi:10.1114/1.205 .

Martin, Ivan, Obradović, Bojana, Freed, LE, Vunjak-Novaković, Gordana, "Method for quantitative analysis of glycosaminoglycan distribution in cultured natural and engineered cartilage" in Annals of Biomedical Engineering, 27, no. 5 (1999):656-662,
https://doi.org/10.1114/1.205 . .

TY  - JOUR
AU  - Obradović, Bojana
AU  - Carrier, RL
AU  - Vunjak-Novaković, Gordana
AU  - Freed, LE
PY  - 1999
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/249
AB  - Tissue engineered cartilage can be grown in vitro if the necessary physical and biochemical factors are present in the tissue culture environment. Cell metabolism and tissue composition were studied for engineered cartilage cultured for 5 weeks using bovine articular chondrocytes, polymer scaffolds (5 mm diameter x 2 mm thick fibrous discs), and rotating bioreactors. Medium pH and concentrations of oxygen, carbon dioxide, glucose, lactate, ammonia, and glycosoaminoglycan (GAG) were varied by altering the exchange rates of gas and medium in the bioreactors. Cell-polymer constructs were assessed with respect to histomorphology, biochemical composition and metabolic activity. Low oxygen tension (similar to 40 mmHg) and low pH (similar to 6.7) were associated with anaerobic cell metabolism (yield of lactate on glucose, Y-L/G, of 2.2 mol/mol) while higher oxygen tension (similar to 80 mmHg) and higher pH (similar to 7.0) were associated with more aerobic cell metabolism (Y-L/G of 1.65-1.79 mol/mol). Under conditions of infrequent medium replacement (50% once per week), cells utilized more economical pathways such that glucose consumption and lactate production both decreased, cell metabolism remained relatively aerobic (Y-L/G of 1.67 mol/mol) and the resulting constructs were cartilaginous. More aerobic conditions generally resulted in larger constructs containing higher amounts of cartilaginous tissue components, while anaerobic conditions suppressed chondrogenesis in 3D tissue constructs.
PB  - John Wiley & Sons Inc, New York
T2  - Biotechnology and Bioengineering
T1  - Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage
EP  - 205
IS  - 2
SP  - 197
VL  - 63
DO  - 10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2
ER  - 

@article{
author = "Obradović, Bojana and Carrier, RL and Vunjak-Novaković, Gordana and Freed, LE",
year = "1999",
abstract = "Tissue engineered cartilage can be grown in vitro if the necessary physical and biochemical factors are present in the tissue culture environment. Cell metabolism and tissue composition were studied for engineered cartilage cultured for 5 weeks using bovine articular chondrocytes, polymer scaffolds (5 mm diameter x 2 mm thick fibrous discs), and rotating bioreactors. Medium pH and concentrations of oxygen, carbon dioxide, glucose, lactate, ammonia, and glycosoaminoglycan (GAG) were varied by altering the exchange rates of gas and medium in the bioreactors. Cell-polymer constructs were assessed with respect to histomorphology, biochemical composition and metabolic activity. Low oxygen tension (similar to 40 mmHg) and low pH (similar to 6.7) were associated with anaerobic cell metabolism (yield of lactate on glucose, Y-L/G, of 2.2 mol/mol) while higher oxygen tension (similar to 80 mmHg) and higher pH (similar to 7.0) were associated with more aerobic cell metabolism (Y-L/G of 1.65-1.79 mol/mol). Under conditions of infrequent medium replacement (50% once per week), cells utilized more economical pathways such that glucose consumption and lactate production both decreased, cell metabolism remained relatively aerobic (Y-L/G of 1.67 mol/mol) and the resulting constructs were cartilaginous. More aerobic conditions generally resulted in larger constructs containing higher amounts of cartilaginous tissue components, while anaerobic conditions suppressed chondrogenesis in 3D tissue constructs.",
publisher = "John Wiley & Sons Inc, New York",
journal = "Biotechnology and Bioengineering",
title = "Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage",
pages = "205-197",
number = "2",
volume = "63",
doi = "10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2"
}

Obradović, B., Carrier, R., Vunjak-Novaković, G.,& Freed, L.. (1999). Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage. in Biotechnology and Bioengineering
John Wiley & Sons Inc, New York., 63(2), 197-205.
https://doi.org/10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2

Obradović B, Carrier R, Vunjak-Novaković G, Freed L. Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage. in Biotechnology and Bioengineering. 1999;63(2):197-205.
doi:10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2 .

Obradović, Bojana, Carrier, RL, Vunjak-Novaković, Gordana, Freed, LE, "Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage" in Biotechnology and Bioengineering, 63, no. 2 (1999):197-205,
https://doi.org/10.1002/(SICI)1097-0290(19990420)63:2197::AID-BIT83.0.CO;2-2 . .

TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Martin, Ivan
AU  - Obradović, Bojana
AU  - Treppo, S
AU  - Grodzinsky, AJ
AU  - Langer, R
AU  - Freed, LE
PY  - 1999
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/243
AB  - Cartilaginous constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In the present work, the relationships between the composition and mechanical properties of engineered cartilage constructs were studied by culturing bovine calf articular chondrocytes on fibrous polyglycolic acid scaffolds (5 mm in diameter, 2-mm thick, and 97% porous) in three different environments: static flasks, mixed flasks, and rotating vessels. After 6 weeks of cultivation, the composition, morphology, and mechanical function of the constructs in radially confined static and dynamic compression all depended on the conditions of in vitro cultivation. Static culture yielded small and fragile constructs, while turbulent flow in mixed flasks yielded constructs with fibrous outer capsules; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels were the largest, contained continuous cartilage-like extracellular matrices with the highest fractions of glycosaminoglycan and collagen, and had the best mechanical properties. The equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential correlated with the wet-weight fractions of glycosaminoglycan, collagen, and water. These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage.
PB  - Wiley, Hoboken
T2  - Journal of Orthopaedic Research
T1  - Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage
EP  - 138
IS  - 1
SP  - 130
VL  - 17
DO  - 10.1002/jor.1100170119
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Martin, Ivan and Obradović, Bojana and Treppo, S and Grodzinsky, AJ and Langer, R and Freed, LE",
year = "1999",
abstract = "Cartilaginous constructs have been grown in vitro with use of isolated cells, biodegradable polymer scaffolds, and bioreactors. In the present work, the relationships between the composition and mechanical properties of engineered cartilage constructs were studied by culturing bovine calf articular chondrocytes on fibrous polyglycolic acid scaffolds (5 mm in diameter, 2-mm thick, and 97% porous) in three different environments: static flasks, mixed flasks, and rotating vessels. After 6 weeks of cultivation, the composition, morphology, and mechanical function of the constructs in radially confined static and dynamic compression all depended on the conditions of in vitro cultivation. Static culture yielded small and fragile constructs, while turbulent flow in mixed flasks yielded constructs with fibrous outer capsules; both environments resulted in constructs with poor mechanical properties. The constructs that were cultured freely suspended in a dynamic laminar flow field in rotating vessels were the largest, contained continuous cartilage-like extracellular matrices with the highest fractions of glycosaminoglycan and collagen, and had the best mechanical properties. The equilibrium modulus, hydraulic permeability, dynamic stiffness, and streaming potential correlated with the wet-weight fractions of glycosaminoglycan, collagen, and water. These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage.",
publisher = "Wiley, Hoboken",
journal = "Journal of Orthopaedic Research",
title = "Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage",
pages = "138-130",
number = "1",
volume = "17",
doi = "10.1002/jor.1100170119"
}

Vunjak-Novaković, G., Martin, I., Obradović, B., Treppo, S., Grodzinsky, A., Langer, R.,& Freed, L.. (1999). Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. in Journal of Orthopaedic Research
Wiley, Hoboken., 17(1), 130-138.
https://doi.org/10.1002/jor.1100170119

Vunjak-Novaković G, Martin I, Obradović B, Treppo S, Grodzinsky A, Langer R, Freed L. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. in Journal of Orthopaedic Research. 1999;17(1):130-138.
doi:10.1002/jor.1100170119 .

Vunjak-Novaković, Gordana, Martin, Ivan, Obradović, Bojana, Treppo, S, Grodzinsky, AJ, Langer, R, Freed, LE, "Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage" in Journal of Orthopaedic Research, 17, no. 1 (1999):130-138,
https://doi.org/10.1002/jor.1100170119 . .

TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Obradović, Bojana
AU  - Martin, Ivan
AU  - Bursac, PM
AU  - Langer, R
AU  - Freed, LE
PY  - 1998
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/193
AB  - Cell seeding of three-dimensional polymer scaffolds is the first step of the cultivation of engineered tissues in bioreactors. Seeding requirements of large scaffolds to make implants for potential clinical use include: (a) high yield, to maximize the utilization of donor cells, (b) high kinetic rate, to minimize the time in suspension for anchorage-dependent and shear-sensitive cells, and
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering
EP  - 202
IS  - 2
SP  - 193
VL  - 14
DO  - 10.1021/bp970120j
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Obradović, Bojana and Martin, Ivan and Bursac, PM and Langer, R and Freed, LE",
year = "1998",
abstract = "Cell seeding of three-dimensional polymer scaffolds is the first step of the cultivation of engineered tissues in bioreactors. Seeding requirements of large scaffolds to make implants for potential clinical use include: (a) high yield, to maximize the utilization of donor cells, (b) high kinetic rate, to minimize the time in suspension for anchorage-dependent and shear-sensitive cells, and",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering",
pages = "202-193",
number = "2",
volume = "14",
doi = "10.1021/bp970120j"
}

Vunjak-Novaković, G., Obradović, B., Martin, I., Bursac, P., Langer, R.,& Freed, L.. (1998). Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. in Biotechnology Progress
Wiley, Hoboken., 14(2), 193-202.
https://doi.org/10.1021/bp970120j

Vunjak-Novaković G, Obradović B, Martin I, Bursac P, Langer R, Freed L. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. in Biotechnology Progress. 1998;14(2):193-202.
doi:10.1021/bp970120j .

Vunjak-Novaković, Gordana, Obradović, Bojana, Martin, Ivan, Bursac, PM, Langer, R, Freed, LE, "Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering" in Biotechnology Progress, 14, no. 2 (1998):193-202,
https://doi.org/10.1021/bp970120j . .

TY  - JOUR
AU  - Sajc, L.
AU  - Jovanović, Z.
AU  - Jovanović, G.
AU  - Bugarski, Branko
AU  - Vunjak-Novaković, Gordana
PY  - 1996
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/98
AB  - The study concerns a phenomenon in which the magnetization of ferromagnetic particles stabilizes the state of uniform fluidization in gas-solid, liquid-solid and gas-liquid-solid fluidized beds. Fluidization regimes and bed structure are functions of the intensity of an external magnetic field and the magnetic propererties of the particles. A fluidized bed of magnetized ferromagnetic particles can be regarded as a ferromagnet, with the permeability proportional to the ferromagnetic material magnetization properties, the intensity of the magnetic field applied by the solenoid, particle diameter and fluidized bed porosity. Stability analysis was performed using mean field theory to determine the magnetic forces acting at the gas-ferromagnet and liquid-ferromagnet surface, a discontinuity boundary, and to predict the mechanism of transition from the unstably fluidized to the stably fluidized sate.
PB  - Serbian Chemical Society, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - The interfacial stability of magnetically stabilized fluidized beds
EP  - 329
IS  - 4-5
SP  - 319
VL  - 61
UR  - https://hdl.handle.net/21.15107/rcub_technorep_98
ER  - 

@article{
author = "Sajc, L. and Jovanović, Z. and Jovanović, G. and Bugarski, Branko and Vunjak-Novaković, Gordana",
year = "1996",
abstract = "The study concerns a phenomenon in which the magnetization of ferromagnetic particles stabilizes the state of uniform fluidization in gas-solid, liquid-solid and gas-liquid-solid fluidized beds. Fluidization regimes and bed structure are functions of the intensity of an external magnetic field and the magnetic propererties of the particles. A fluidized bed of magnetized ferromagnetic particles can be regarded as a ferromagnet, with the permeability proportional to the ferromagnetic material magnetization properties, the intensity of the magnetic field applied by the solenoid, particle diameter and fluidized bed porosity. Stability analysis was performed using mean field theory to determine the magnetic forces acting at the gas-ferromagnet and liquid-ferromagnet surface, a discontinuity boundary, and to predict the mechanism of transition from the unstably fluidized to the stably fluidized sate.",
publisher = "Serbian Chemical Society, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "The interfacial stability of magnetically stabilized fluidized beds",
pages = "329-319",
number = "4-5",
volume = "61",
url = "https://hdl.handle.net/21.15107/rcub_technorep_98"
}

Sajc, L., Jovanović, Z., Jovanović, G., Bugarski, B.,& Vunjak-Novaković, G.. (1996). The interfacial stability of magnetically stabilized fluidized beds. in Journal of the Serbian Chemical Society
Serbian Chemical Society, Belgrade., 61(4-5), 319-329.
https://hdl.handle.net/21.15107/rcub_technorep_98

Sajc L, Jovanović Z, Jovanović G, Bugarski B, Vunjak-Novaković G. The interfacial stability of magnetically stabilized fluidized beds. in Journal of the Serbian Chemical Society. 1996;61(4-5):319-329.
https://hdl.handle.net/21.15107/rcub_technorep_98 .

Sajc, L., Jovanović, Z., Jovanović, G., Bugarski, Branko, Vunjak-Novaković, Gordana, "The interfacial stability of magnetically stabilized fluidized beds" in Journal of the Serbian Chemical Society, 61, no. 4-5 (1996):319-329,
https://hdl.handle.net/21.15107/rcub_technorep_98 .

TY  - JOUR
AU  - Kundaković, Lj.
AU  - Obradović, Bojana
AU  - Vunjak-Novaković, Gordana
PY  - 1996
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/85
AB  - Three phase flow and mixing in the liquid phase were studied for a draft tube fluidized bed (DTFB) operating in a circulating regime. The DTFB was considered as a system consisting of five interconnected compartiments: the draft tube, annulus and three top sections. The local liquid velocities and phase hold ups were assessed from (a) tracer response studies using a 3-D column, and (b) flow visualization studies using a geometrically similar 2-D column. The drift-flux model for two-phase flow was extended to take into account the momentum transfer between gas bubbles, liquid and solid particles and used to describe the circulating flow in the DTFB. Additional momentum transfer from gas bubbles to solid particles was identified and attributed to the changes in relative velocities and drag forces induced by bubble motion in three phase flow. Mixing in the liquid phase was assessed in the individual DTFB compartments and the system as a whole using the response technique and fast conductivity measurements. Axial mixing in the liquid phase was attributed to the circulating flow between the draft tube and annulus, and to the backmixing in the top sections of the reactor.
PB  - Serbian Chemical Society, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Fluid dynamic studies of a three-phase fluidized bed
EP  - 310
IS  - 4-5
SP  - 297
VL  - 61
UR  - https://hdl.handle.net/21.15107/rcub_technorep_85
ER  - 

@article{
author = "Kundaković, Lj. and Obradović, Bojana and Vunjak-Novaković, Gordana",
year = "1996",
abstract = "Three phase flow and mixing in the liquid phase were studied for a draft tube fluidized bed (DTFB) operating in a circulating regime. The DTFB was considered as a system consisting of five interconnected compartiments: the draft tube, annulus and three top sections. The local liquid velocities and phase hold ups were assessed from (a) tracer response studies using a 3-D column, and (b) flow visualization studies using a geometrically similar 2-D column. The drift-flux model for two-phase flow was extended to take into account the momentum transfer between gas bubbles, liquid and solid particles and used to describe the circulating flow in the DTFB. Additional momentum transfer from gas bubbles to solid particles was identified and attributed to the changes in relative velocities and drag forces induced by bubble motion in three phase flow. Mixing in the liquid phase was assessed in the individual DTFB compartments and the system as a whole using the response technique and fast conductivity measurements. Axial mixing in the liquid phase was attributed to the circulating flow between the draft tube and annulus, and to the backmixing in the top sections of the reactor.",
publisher = "Serbian Chemical Society, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Fluid dynamic studies of a three-phase fluidized bed",
pages = "310-297",
number = "4-5",
volume = "61",
url = "https://hdl.handle.net/21.15107/rcub_technorep_85"
}

Kundaković, Lj., Obradović, B.,& Vunjak-Novaković, G.. (1996). Fluid dynamic studies of a three-phase fluidized bed. in Journal of the Serbian Chemical Society
Serbian Chemical Society, Belgrade., 61(4-5), 297-310.
https://hdl.handle.net/21.15107/rcub_technorep_85

Kundaković L, Obradović B, Vunjak-Novaković G. Fluid dynamic studies of a three-phase fluidized bed. in Journal of the Serbian Chemical Society. 1996;61(4-5):297-310.
https://hdl.handle.net/21.15107/rcub_technorep_85 .

Kundaković, Lj., Obradović, Bojana, Vunjak-Novaković, Gordana, "Fluid dynamic studies of a three-phase fluidized bed" in Journal of the Serbian Chemical Society, 61, no. 4-5 (1996):297-310,
https://hdl.handle.net/21.15107/rcub_technorep_85 .

TY  - JOUR
AU  - Sajc, L.
AU  - Vunjak-Novaković, Gordana
AU  - Grubisić, D.
AU  - Kovačević, N.
AU  - Vuković, Dragan
AU  - Bugarski, Branko
PY  - 1995
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/60
AB  - The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor
EP  - 423
IS  - 3
SP  - 416
VL  - 43
DO  - 10.1007/BF00218443
ER  - 

@article{
author = "Sajc, L. and Vunjak-Novaković, Gordana and Grubisić, D. and Kovačević, N. and Vuković, Dragan and Bugarski, Branko",
year = "1995",
abstract = "The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor",
pages = "423-416",
number = "3",
volume = "43",
doi = "10.1007/BF00218443"
}

Sajc, L., Vunjak-Novaković, G., Grubisić, D., Kovačević, N., Vuković, D.,& Bugarski, B.. (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology
Springer, New York., 43(3), 416-423.
https://doi.org/10.1007/BF00218443

Sajc L, Vunjak-Novaković G, Grubisić D, Kovačević N, Vuković D, Bugarski B. Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. in Applied Microbiology and Biotechnology. 1995;43(3):416-423.
doi:10.1007/BF00218443 .

Sajc, L., Vunjak-Novaković, Gordana, Grubisić, D., Kovačević, N., Vuković, Dragan, Bugarski, Branko, "Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor" in Applied Microbiology and Biotechnology, 43, no. 3 (1995):416-423,
https://doi.org/10.1007/BF00218443 . .