TY  - JOUR
AU  - Janković, Ana
AU  - Eraković, Sanja
AU  - Ristoscu, Carmen
AU  - Mihailescu, Ion N.
AU  - Duta, L.
AU  - Visan, A.
AU  - Stan, George E.
AU  - Popa, A. C.
AU  - Husanu, M. A.
AU  - Luculescu, C. R.
AU  - Srdić, Vladimir V.
AU  - Janaćković, Đorđe
AU  - Mišković-Stanković, Vesna
AU  - Bleotu, C.
AU  - Chifiriuc, M. C.
AU  - Mihailescu, Ion N.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3042
AB  - We report on thin film deposition by matrix-assisted pulsed laser evaporation of simple hydroxyapatite (HA) or silver (Ag) doped HA combined with the natural biopolymer organosolv lignin (Lig) (Ag:HA-Lig). Solid cryogenic target of aqueous dispersions of Ag:HA-Lig composite and its counterpart without silver (HA-Lig) were prepared for evaporation using a KrF* excimer laser source. The expulsed material was assembled onto TiO2/Ti substrata or silicon wafers and subjected to physical-chemical investigations. Smooth, uniform films adherent to substratum were observed. The chemical analyses confirmed the presence of the HA components, but also evidenced traces of Ag and Lig. Deposited HA was Ca deficient, which is indicative of a film with increased solubility. Recorded X-ray Diffraction patterns were characteristic for amorphous films. Lig presence in thin films was undoubtedly proved by both X-ray Photoelectron and Fourier Transform Infra-Red Spectroscopy analyses. The microbiological evaluation showed that the newly assembled surfaces exhibited an inhibitory activity both on the initial steps of biofilm forming, and on mature bacterial and fungal biofilm development. The intensity of the antibiofilm activity was positively influenced by the presence of the Lig and/or Ag, in the case of Staphylococcus aureus, Pseudomonas aeruginosa and Candida famata biofilms. The obtained surfaces exhibited a low cytotoxicity toward human mesenchymal stem cells, being therefore promising candidates for fabricating implantable biomaterials with increased biocompatibility and resistance to microbial colonization and further biofilm development.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation
IS  - 1
VL  - 26
DO  - 10.1007/s10856-014-5333-y
ER  - 

@article{
author = "Janković, Ana and Eraković, Sanja and Ristoscu, Carmen and Mihailescu, Ion N. and Duta, L. and Visan, A. and Stan, George E. and Popa, A. C. and Husanu, M. A. and Luculescu, C. R. and Srdić, Vladimir V. and Janaćković, Đorđe and Mišković-Stanković, Vesna and Bleotu, C. and Chifiriuc, M. C. and Mihailescu, Ion N.",
year = "2015",
abstract = "We report on thin film deposition by matrix-assisted pulsed laser evaporation of simple hydroxyapatite (HA) or silver (Ag) doped HA combined with the natural biopolymer organosolv lignin (Lig) (Ag:HA-Lig). Solid cryogenic target of aqueous dispersions of Ag:HA-Lig composite and its counterpart without silver (HA-Lig) were prepared for evaporation using a KrF* excimer laser source. The expulsed material was assembled onto TiO2/Ti substrata or silicon wafers and subjected to physical-chemical investigations. Smooth, uniform films adherent to substratum were observed. The chemical analyses confirmed the presence of the HA components, but also evidenced traces of Ag and Lig. Deposited HA was Ca deficient, which is indicative of a film with increased solubility. Recorded X-ray Diffraction patterns were characteristic for amorphous films. Lig presence in thin films was undoubtedly proved by both X-ray Photoelectron and Fourier Transform Infra-Red Spectroscopy analyses. The microbiological evaluation showed that the newly assembled surfaces exhibited an inhibitory activity both on the initial steps of biofilm forming, and on mature bacterial and fungal biofilm development. The intensity of the antibiofilm activity was positively influenced by the presence of the Lig and/or Ag, in the case of Staphylococcus aureus, Pseudomonas aeruginosa and Candida famata biofilms. The obtained surfaces exhibited a low cytotoxicity toward human mesenchymal stem cells, being therefore promising candidates for fabricating implantable biomaterials with increased biocompatibility and resistance to microbial colonization and further biofilm development.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation",
number = "1",
volume = "26",
doi = "10.1007/s10856-014-5333-y"
}

Janković, A., Eraković, S., Ristoscu, C., Mihailescu, I. N., Duta, L., Visan, A., Stan, G. E., Popa, A. C., Husanu, M. A., Luculescu, C. R., Srdić, V. V., Janaćković, Đ., Mišković-Stanković, V., Bleotu, C., Chifiriuc, M. C.,& Mihailescu, I. N.. (2015). Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 26(1).
https://doi.org/10.1007/s10856-014-5333-y

Janković A, Eraković S, Ristoscu C, Mihailescu IN, Duta L, Visan A, Stan GE, Popa AC, Husanu MA, Luculescu CR, Srdić VV, Janaćković Đ, Mišković-Stanković V, Bleotu C, Chifiriuc MC, Mihailescu IN. Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation. in Journal of Materials Science-Materials in Medicine. 2015;26(1).
doi:10.1007/s10856-014-5333-y .

Janković, Ana, Eraković, Sanja, Ristoscu, Carmen, Mihailescu, Ion N., Duta, L., Visan, A., Stan, George E., Popa, A. C., Husanu, M. A., Luculescu, C. R., Srdić, Vladimir V., Janaćković, Đorđe, Mišković-Stanković, Vesna, Bleotu, C., Chifiriuc, M. C., Mihailescu, Ion N., "Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation" in Journal of Materials Science-Materials in Medicine, 26, no. 1 (2015),
https://doi.org/10.1007/s10856-014-5333-y . .

TY  - JOUR
AU  - Eraković, Sanja
AU  - Janković, Ana
AU  - Ristoscu, Carmen
AU  - Duta, L.
AU  - Serban, N.
AU  - Visan, A.
AU  - Mihailescu, Ion N.
AU  - Stan, George E.
AU  - Socol, M.
AU  - Iordache, O.
AU  - Dumitrescu, I.
AU  - Luculescu, C. R.
AU  - Janaćković, Đorđe
AU  - Mišković-Stanković, Vesna
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2816
AB  - Hydroxyapatite (HA) is a widely used biomaterial for implant thin films, largely recognized for its excellent capability to chemically bond to hard tissue inducing the osteogenesis without immune response from human tissues. Nowadays, intense research efforts are focused on development of antimicrobial HA doped thin films. In particular, HA doped with Ag (Ag:HA) is expected to inhibit the attachment of microbes and contamination of metallic implant surface. We herewith report on nano-sized HA and Ag:HA thin films synthesized by pulsed laser deposition on pure Ti and Ti modified with 100 nm diameter TiO2 nanotubes (fabricated by anodization of Ti plates) substrates. The HA-based thin films were characterized by SEM, AFM, EDS, FUR, and XRD. The cytotoxic activity was tested with HEp2 cells against controls. The antifungal efficiency of the deposited layers was tested against the Candida albicans and Aspergillus niger strains. The Ti substrates modified with TiO2 nanotubes covered with Ag:HA thin films showed the highest antifungal activity.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Surface Science
T1  - Antifungal activity of Ag:hydroxyapatite thin films synthesized by pulsed laser deposition on Ti and Ti modified by TiO2 nanotubes substrates
EP  - 45
SP  - 37
VL  - 293
DO  - 10.1016/j.apsusc.2013.12.029
ER  - 

@article{
author = "Eraković, Sanja and Janković, Ana and Ristoscu, Carmen and Duta, L. and Serban, N. and Visan, A. and Mihailescu, Ion N. and Stan, George E. and Socol, M. and Iordache, O. and Dumitrescu, I. and Luculescu, C. R. and Janaćković, Đorđe and Mišković-Stanković, Vesna",
year = "2014",
abstract = "Hydroxyapatite (HA) is a widely used biomaterial for implant thin films, largely recognized for its excellent capability to chemically bond to hard tissue inducing the osteogenesis without immune response from human tissues. Nowadays, intense research efforts are focused on development of antimicrobial HA doped thin films. In particular, HA doped with Ag (Ag:HA) is expected to inhibit the attachment of microbes and contamination of metallic implant surface. We herewith report on nano-sized HA and Ag:HA thin films synthesized by pulsed laser deposition on pure Ti and Ti modified with 100 nm diameter TiO2 nanotubes (fabricated by anodization of Ti plates) substrates. The HA-based thin films were characterized by SEM, AFM, EDS, FUR, and XRD. The cytotoxic activity was tested with HEp2 cells against controls. The antifungal efficiency of the deposited layers was tested against the Candida albicans and Aspergillus niger strains. The Ti substrates modified with TiO2 nanotubes covered with Ag:HA thin films showed the highest antifungal activity.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Surface Science",
title = "Antifungal activity of Ag:hydroxyapatite thin films synthesized by pulsed laser deposition on Ti and Ti modified by TiO2 nanotubes substrates",
pages = "45-37",
volume = "293",
doi = "10.1016/j.apsusc.2013.12.029"
}

Eraković, S., Janković, A., Ristoscu, C., Duta, L., Serban, N., Visan, A., Mihailescu, I. N., Stan, G. E., Socol, M., Iordache, O., Dumitrescu, I., Luculescu, C. R., Janaćković, Đ.,& Mišković-Stanković, V.. (2014). Antifungal activity of Ag:hydroxyapatite thin films synthesized by pulsed laser deposition on Ti and Ti modified by TiO2 nanotubes substrates. in Applied Surface Science
Elsevier Science Bv, Amsterdam., 293, 37-45.
https://doi.org/10.1016/j.apsusc.2013.12.029

Eraković S, Janković A, Ristoscu C, Duta L, Serban N, Visan A, Mihailescu IN, Stan GE, Socol M, Iordache O, Dumitrescu I, Luculescu CR, Janaćković Đ, Mišković-Stanković V. Antifungal activity of Ag:hydroxyapatite thin films synthesized by pulsed laser deposition on Ti and Ti modified by TiO2 nanotubes substrates. in Applied Surface Science. 2014;293:37-45.
doi:10.1016/j.apsusc.2013.12.029 .

Eraković, Sanja, Janković, Ana, Ristoscu, Carmen, Duta, L., Serban, N., Visan, A., Mihailescu, Ion N., Stan, George E., Socol, M., Iordache, O., Dumitrescu, I., Luculescu, C. R., Janaćković, Đorđe, Mišković-Stanković, Vesna, "Antifungal activity of Ag:hydroxyapatite thin films synthesized by pulsed laser deposition on Ti and Ti modified by TiO2 nanotubes substrates" in Applied Surface Science, 293 (2014):37-45,
https://doi.org/10.1016/j.apsusc.2013.12.029 . .

TY  - JOUR
AU  - Linnik, Oksana
AU  - Petrik, Irina
AU  - Smirnova, Natalia
AU  - Kandyba, Viktor
AU  - Korduban, Oleksandr
AU  - Eremenko, Anna
AU  - Socol, Gabriel
AU  - Stefan, Nicolaie
AU  - Ristoscu, Carmen
AU  - Mihailescu, Ion N.
AU  - Sutan, Claudiu
AU  - Malinovschi, Viorel
AU  - Đokić, Veljko
AU  - Janaković, Đordje
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2007
AB  - Doped TiO2/ZrO2 films were obtained by Pulsed Laser Deposition method under different synthesis conditions. The onset of absorption spectra was red shifted for the films obtained in N-2 containing gas mixtures, while a broad absorption in visible was observed in the case of films deposited in CH4 atmosphere. The presence of O-Ti-N bonds revealed by XPS corresponded to the highest photocatalytic performance. XPS spectra of the samples obtained in N-2/CH4 gas mixtures evidenced a more effective incorporation of nitrogen in the structure due to oxygen deficiency. Nevertheless, no atomic carbon presence in the TiO2/ZrO2 structures has been detected.
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Tio2/zro2 thin films synthesized by pld in low pressure n-, c- and/or o-containing gases: structural, optical and photocatalytic properties
EP  - 1352
IS  - 3
SP  - 1343
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2007
ER  - 

@article{
author = "Linnik, Oksana and Petrik, Irina and Smirnova, Natalia and Kandyba, Viktor and Korduban, Oleksandr and Eremenko, Anna and Socol, Gabriel and Stefan, Nicolaie and Ristoscu, Carmen and Mihailescu, Ion N. and Sutan, Claudiu and Malinovschi, Viorel and Đokić, Veljko and Janaković, Đordje",
year = "2012",
abstract = "Doped TiO2/ZrO2 films were obtained by Pulsed Laser Deposition method under different synthesis conditions. The onset of absorption spectra was red shifted for the films obtained in N-2 containing gas mixtures, while a broad absorption in visible was observed in the case of films deposited in CH4 atmosphere. The presence of O-Ti-N bonds revealed by XPS corresponded to the highest photocatalytic performance. XPS spectra of the samples obtained in N-2/CH4 gas mixtures evidenced a more effective incorporation of nitrogen in the structure due to oxygen deficiency. Nevertheless, no atomic carbon presence in the TiO2/ZrO2 structures has been detected.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Tio2/zro2 thin films synthesized by pld in low pressure n-, c- and/or o-containing gases: structural, optical and photocatalytic properties",
pages = "1352-1343",
number = "3",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2007"
}

Linnik, O., Petrik, I., Smirnova, N., Kandyba, V., Korduban, O., Eremenko, A., Socol, G., Stefan, N., Ristoscu, C., Mihailescu, I. N., Sutan, C., Malinovschi, V., Đokić, V.,& Janaković, Đ.. (2012). Tio2/zro2 thin films synthesized by pld in low pressure n-, c- and/or o-containing gases: structural, optical and photocatalytic properties. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 7(3), 1343-1352.
https://hdl.handle.net/21.15107/rcub_technorep_2007

Linnik O, Petrik I, Smirnova N, Kandyba V, Korduban O, Eremenko A, Socol G, Stefan N, Ristoscu C, Mihailescu IN, Sutan C, Malinovschi V, Đokić V, Janaković Đ. Tio2/zro2 thin films synthesized by pld in low pressure n-, c- and/or o-containing gases: structural, optical and photocatalytic properties. in Digest Journal of Nanomaterials and Biostructures. 2012;7(3):1343-1352.
https://hdl.handle.net/21.15107/rcub_technorep_2007 .

Linnik, Oksana, Petrik, Irina, Smirnova, Natalia, Kandyba, Viktor, Korduban, Oleksandr, Eremenko, Anna, Socol, Gabriel, Stefan, Nicolaie, Ristoscu, Carmen, Mihailescu, Ion N., Sutan, Claudiu, Malinovschi, Viorel, Đokić, Veljko, Janaković, Đordje, "Tio2/zro2 thin films synthesized by pld in low pressure n-, c- and/or o-containing gases: structural, optical and photocatalytic properties" in Digest Journal of Nanomaterials and Biostructures, 7, no. 3 (2012):1343-1352,
https://hdl.handle.net/21.15107/rcub_technorep_2007 .

TY  - JOUR
AU  - Tanasković, Dušanka
AU  - Veljović, Đorđe
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
AU  - Mitrić, Miodrag
AU  - Cojanu, C.
AU  - Ristoscu, Carmen
AU  - Mihailescu, Ion N.
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1220
AB  - Pulsed laser deposition was used to obtain functionally graded bioactive glass coatings on titanium substrates. An UV KrF* (lambda=248 nm, tau gt 7 ns) excimer laser was used for the multi-pulse irradiation of the targets. The depositions were performed in oxygen while keeping substrate temperature at 400 degrees C. We used sintered glass targets in the system SiO2-Na2O-K2O-CaO-MgO-P2O5 that differed in SiO2 content, which was either 57 wt.% (6P57) or 61 wt.% (6P61). A glass 6P61 was used as the first layer in direct contact with the metallic substrate, while the outer bioactive layer was made of glass 6P57. Both the bioactive coatings and the bulk glasses were analyzed by Fourier transform infrared spectrometry (FTIR), grazing incidence X-ray diffraction (GIXRD), and scanning electron microscopy (SEM). The FTIR spectra of the glass powders and glass coatings showed the main vibration modes of the Si-O-Si groups. GIXRD analysis confirmed that the glass coatings had an amorphous structure. The SEM micrographs of the glass coatings showed the films to consist of droplets with diameters ranging from 0.2 to 5 Pm. SEM was used to determine the rate of apatite formation on the coating when exposed to simulated body fluid (SBF) solution for 7 days. We demonstrated that pulsed laser deposition leads to good glass-metal adhesion on the substrate and well attached bioactive particles on the surface. We consider therefore this method appropriate for forming implants that can develop an apatite layer after immersion in SBF.
PB  - Trans Tech Publications Ltd, Durnten-Zurich
T2  - Key Engineering Materials
T1  - Double-layer bioactive glass coatings obtained by pulsed laser deposition
EP  - 280
SP  - 277
VL  - 361-363 I
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6718
ER  - 

@article{
author = "Tanasković, Dušanka and Veljović, Đorđe and Petrović, Rada and Janaćković, Đorđe and Mitrić, Miodrag and Cojanu, C. and Ristoscu, Carmen and Mihailescu, Ion N.",
year = "2008",
abstract = "Pulsed laser deposition was used to obtain functionally graded bioactive glass coatings on titanium substrates. An UV KrF* (lambda=248 nm, tau gt 7 ns) excimer laser was used for the multi-pulse irradiation of the targets. The depositions were performed in oxygen while keeping substrate temperature at 400 degrees C. We used sintered glass targets in the system SiO2-Na2O-K2O-CaO-MgO-P2O5 that differed in SiO2 content, which was either 57 wt.% (6P57) or 61 wt.% (6P61). A glass 6P61 was used as the first layer in direct contact with the metallic substrate, while the outer bioactive layer was made of glass 6P57. Both the bioactive coatings and the bulk glasses were analyzed by Fourier transform infrared spectrometry (FTIR), grazing incidence X-ray diffraction (GIXRD), and scanning electron microscopy (SEM). The FTIR spectra of the glass powders and glass coatings showed the main vibration modes of the Si-O-Si groups. GIXRD analysis confirmed that the glass coatings had an amorphous structure. The SEM micrographs of the glass coatings showed the films to consist of droplets with diameters ranging from 0.2 to 5 Pm. SEM was used to determine the rate of apatite formation on the coating when exposed to simulated body fluid (SBF) solution for 7 days. We demonstrated that pulsed laser deposition leads to good glass-metal adhesion on the substrate and well attached bioactive particles on the surface. We consider therefore this method appropriate for forming implants that can develop an apatite layer after immersion in SBF.",
publisher = "Trans Tech Publications Ltd, Durnten-Zurich",
journal = "Key Engineering Materials",
title = "Double-layer bioactive glass coatings obtained by pulsed laser deposition",
pages = "280-277",
volume = "361-363 I",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6718"
}

Tanasković, D., Veljović, Đ., Petrović, R., Janaćković, Đ., Mitrić, M., Cojanu, C., Ristoscu, C.,& Mihailescu, I. N.. (2008). Double-layer bioactive glass coatings obtained by pulsed laser deposition. in Key Engineering Materials
Trans Tech Publications Ltd, Durnten-Zurich., 361-363 I, 277-280.
https://hdl.handle.net/21.15107/rcub_vinar_6718

Tanasković D, Veljović Đ, Petrović R, Janaćković Đ, Mitrić M, Cojanu C, Ristoscu C, Mihailescu IN. Double-layer bioactive glass coatings obtained by pulsed laser deposition. in Key Engineering Materials. 2008;361-363 I:277-280.
https://hdl.handle.net/21.15107/rcub_vinar_6718 .

Tanasković, Dušanka, Veljović, Đorđe, Petrović, Rada, Janaćković, Đorđe, Mitrić, Miodrag, Cojanu, C., Ristoscu, Carmen, Mihailescu, Ion N., "Double-layer bioactive glass coatings obtained by pulsed laser deposition" in Key Engineering Materials, 361-363 I (2008):277-280,
https://hdl.handle.net/21.15107/rcub_vinar_6718 .