TY  - JOUR
AU  - Janković, Ana
AU  - Eraković, Sanja
AU  - Ristoscu, Carmen
AU  - Mihailescu, Ion N.
AU  - Duta, L.
AU  - Visan, A.
AU  - Stan, George E.
AU  - Popa, A. C.
AU  - Husanu, M. A.
AU  - Luculescu, C. R.
AU  - Srdić, Vladimir V.
AU  - Janaćković, Đorđe
AU  - Mišković-Stanković, Vesna
AU  - Bleotu, C.
AU  - Chifiriuc, M. C.
AU  - Mihailescu, Ion N.
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3042
AB  - We report on thin film deposition by matrix-assisted pulsed laser evaporation of simple hydroxyapatite (HA) or silver (Ag) doped HA combined with the natural biopolymer organosolv lignin (Lig) (Ag:HA-Lig). Solid cryogenic target of aqueous dispersions of Ag:HA-Lig composite and its counterpart without silver (HA-Lig) were prepared for evaporation using a KrF* excimer laser source. The expulsed material was assembled onto TiO2/Ti substrata or silicon wafers and subjected to physical-chemical investigations. Smooth, uniform films adherent to substratum were observed. The chemical analyses confirmed the presence of the HA components, but also evidenced traces of Ag and Lig. Deposited HA was Ca deficient, which is indicative of a film with increased solubility. Recorded X-ray Diffraction patterns were characteristic for amorphous films. Lig presence in thin films was undoubtedly proved by both X-ray Photoelectron and Fourier Transform Infra-Red Spectroscopy analyses. The microbiological evaluation showed that the newly assembled surfaces exhibited an inhibitory activity both on the initial steps of biofilm forming, and on mature bacterial and fungal biofilm development. The intensity of the antibiofilm activity was positively influenced by the presence of the Lig and/or Ag, in the case of Staphylococcus aureus, Pseudomonas aeruginosa and Candida famata biofilms. The obtained surfaces exhibited a low cytotoxicity toward human mesenchymal stem cells, being therefore promising candidates for fabricating implantable biomaterials with increased biocompatibility and resistance to microbial colonization and further biofilm development.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation
IS  - 1
VL  - 26
DO  - 10.1007/s10856-014-5333-y
ER  - 

@article{
author = "Janković, Ana and Eraković, Sanja and Ristoscu, Carmen and Mihailescu, Ion N. and Duta, L. and Visan, A. and Stan, George E. and Popa, A. C. and Husanu, M. A. and Luculescu, C. R. and Srdić, Vladimir V. and Janaćković, Đorđe and Mišković-Stanković, Vesna and Bleotu, C. and Chifiriuc, M. C. and Mihailescu, Ion N.",
year = "2015",
abstract = "We report on thin film deposition by matrix-assisted pulsed laser evaporation of simple hydroxyapatite (HA) or silver (Ag) doped HA combined with the natural biopolymer organosolv lignin (Lig) (Ag:HA-Lig). Solid cryogenic target of aqueous dispersions of Ag:HA-Lig composite and its counterpart without silver (HA-Lig) were prepared for evaporation using a KrF* excimer laser source. The expulsed material was assembled onto TiO2/Ti substrata or silicon wafers and subjected to physical-chemical investigations. Smooth, uniform films adherent to substratum were observed. The chemical analyses confirmed the presence of the HA components, but also evidenced traces of Ag and Lig. Deposited HA was Ca deficient, which is indicative of a film with increased solubility. Recorded X-ray Diffraction patterns were characteristic for amorphous films. Lig presence in thin films was undoubtedly proved by both X-ray Photoelectron and Fourier Transform Infra-Red Spectroscopy analyses. The microbiological evaluation showed that the newly assembled surfaces exhibited an inhibitory activity both on the initial steps of biofilm forming, and on mature bacterial and fungal biofilm development. The intensity of the antibiofilm activity was positively influenced by the presence of the Lig and/or Ag, in the case of Staphylococcus aureus, Pseudomonas aeruginosa and Candida famata biofilms. The obtained surfaces exhibited a low cytotoxicity toward human mesenchymal stem cells, being therefore promising candidates for fabricating implantable biomaterials with increased biocompatibility and resistance to microbial colonization and further biofilm development.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation",
number = "1",
volume = "26",
doi = "10.1007/s10856-014-5333-y"
}

Janković, A., Eraković, S., Ristoscu, C., Mihailescu, I. N., Duta, L., Visan, A., Stan, G. E., Popa, A. C., Husanu, M. A., Luculescu, C. R., Srdić, V. V., Janaćković, Đ., Mišković-Stanković, V., Bleotu, C., Chifiriuc, M. C.,& Mihailescu, I. N.. (2015). Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 26(1).
https://doi.org/10.1007/s10856-014-5333-y

Janković A, Eraković S, Ristoscu C, Mihailescu IN, Duta L, Visan A, Stan GE, Popa AC, Husanu MA, Luculescu CR, Srdić VV, Janaćković Đ, Mišković-Stanković V, Bleotu C, Chifiriuc MC, Mihailescu IN. Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation. in Journal of Materials Science-Materials in Medicine. 2015;26(1).
doi:10.1007/s10856-014-5333-y .

Janković, Ana, Eraković, Sanja, Ristoscu, Carmen, Mihailescu, Ion N., Duta, L., Visan, A., Stan, George E., Popa, A. C., Husanu, M. A., Luculescu, C. R., Srdić, Vladimir V., Janaćković, Đorđe, Mišković-Stanković, Vesna, Bleotu, C., Chifiriuc, M. C., Mihailescu, Ion N., "Structural and biological evaluation of lignin addition to simple and silver-doped hydroxyapatite thin films synthesized by matrix-assisted pulsed laser evaporation" in Journal of Materials Science-Materials in Medicine, 26, no. 1 (2015),
https://doi.org/10.1007/s10856-014-5333-y . .