TY  - JOUR
AU  - Čolić, Miodrag
AU  - Dzopalić, Tanja
AU  - Tomić, Sergej
AU  - Rajković, Jelena
AU  - Rudolf, Rebeka
AU  - Vuković, Goran D.
AU  - Marinković, Aleksandar
AU  - Uskoković, Petar
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2782
AB  - The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Carbon
T1  - Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells
EP  - 287
SP  - 273
VL  - 67
DO  - 10.1016/j.carbon.2013.09.090
ER  - 

@article{
author = "Čolić, Miodrag and Dzopalić, Tanja and Tomić, Sergej and Rajković, Jelena and Rudolf, Rebeka and Vuković, Goran D. and Marinković, Aleksandar and Uskoković, Petar",
year = "2014",
abstract = "The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs. Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG. In conclusion, our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Carbon",
title = "Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells",
pages = "287-273",
volume = "67",
doi = "10.1016/j.carbon.2013.09.090"
}

Čolić, M., Dzopalić, T., Tomić, S., Rajković, J., Rudolf, R., Vuković, G. D., Marinković, A.,& Uskoković, P.. (2014). Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. in Carbon
Pergamon-Elsevier Science Ltd, Oxford., 67, 273-287.
https://doi.org/10.1016/j.carbon.2013.09.090

Čolić M, Dzopalić T, Tomić S, Rajković J, Rudolf R, Vuković GD, Marinković A, Uskoković P. Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. in Carbon. 2014;67:273-287.
doi:10.1016/j.carbon.2013.09.090 .

Čolić, Miodrag, Dzopalić, Tanja, Tomić, Sergej, Rajković, Jelena, Rudolf, Rebeka, Vuković, Goran D., Marinković, Aleksandar, Uskoković, Petar, "Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells" in Carbon, 67 (2014):273-287,
https://doi.org/10.1016/j.carbon.2013.09.090 . .