TY  - CONF
AU  - Knežević, Magdalena
AU  - Stajković-Srbinović, Olivera
AU  - Maibassova, Assel
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Delić, Dušica
AU  - Buntić, Aneta
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6434
AB  - The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.
PB  - MDPI
C3  - 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
T1  - Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6434
ER  - 

@conference{
author = "Knežević, Magdalena and Stajković-Srbinović, Olivera and Maibassova, Assel and Milić, Marija and Mihajlovski, Katarina and Delić, Dušica and Buntić, Aneta",
year = "2022",
abstract = "The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.",
publisher = "MDPI",
journal = "1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection",
title = "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6434"
}

Knežević, M., Stajković-Srbinović, O., Maibassova, A., Milić, M., Mihajlovski, K., Delić, D.,& Buntić, A.. (2022). Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
MDPI..
https://hdl.handle.net/21.15107/rcub_technorep_6434

Knežević M, Stajković-Srbinović O, Maibassova A, Milić M, Mihajlovski K, Delić D, Buntić A. Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

Knežević, Magdalena, Stajković-Srbinović, Olivera, Maibassova, Assel, Milić, Marija, Mihajlovski, Katarina, Delić, Dušica, Buntić, Aneta, "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades" in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

TY  - CONF
AU  - Buntić, Aneta
AU  - Tošić Jojević, Sonja
AU  - Knežević, Magdalena
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Koković, Nikola
AU  - Saljnikov, Elmira
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6426
AB  - The purpose of this study was to evaluate the effect of biomass ash from combustion of soybean straw
alone or in combination with bacterial inoculums on yield of barley crop, and on soil and plant quality.
The greenhouse pot experiment included four treatments and two control soils (control without any
amendment - ØØ and control with mineral fertilizer ØØ+CAN, 0.3g CAN/pot). Treatments included
soil (3 kg/pot) and biomass ash (30 g/pot) without and with one of three selected bacterial inoculums.
Bacterial strains Streptomyces fulvissimus CKS7, Hymenobacter sp. CKS3 and Sinorhizobium (Ensifer)
meliloti 207 were used for three inoculums preparation (CKS7, CKS7+CKS3 and 207). The ash and
experimental soil samples, as well as soil and plant samples collected at the stage of crop maturity
were tested for: total N, C, S, plant available P2O5 and K2O and for the content of potentially toxic and
hazardous microelements (As, Cd, Сo, Cr, Cu, Mn, Ni, Pb, Zn). The results showed that the addition of
biomass ash significantly reduced soil acidity and increased content of plant available P and K
compared to the controls (in all treatments). Barley yield was almost twice as higher in ash treatment
and by 1.56, 1.70 and 1.81 times higher in treatments Ash+CKS7, Ash+CKS7+CKS3 and Ash+207
respectively, compared to control (ØØ). The content of potentially toxic microelements was below the
maximum allowed concentrations in the soils and shoots. The use of combinations of ash and bacterial
inoculum improved the quality of barley in terms of nitrogen content. This study showed that biomass
ash can be used as fertilizer on acidic soils with low nutrient content alone or in combination with
applied inoculants and provide an environmentally friendly approach in agriculture to reduce the
need for chemical fertilizers and solve the problem of biomass ash disposal.
PB  - Federation of Eurasian Soil Science Societies
C3  - The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey
T1  - The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality
SP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6426
ER  - 

@conference{
author = "Buntić, Aneta and Tošić Jojević, Sonja and Knežević, Magdalena and Milić, Marija and Mihajlovski, Katarina and Koković, Nikola and Saljnikov, Elmira",
year = "2021",
abstract = "The purpose of this study was to evaluate the effect of biomass ash from combustion of soybean straw
alone or in combination with bacterial inoculums on yield of barley crop, and on soil and plant quality.
The greenhouse pot experiment included four treatments and two control soils (control without any
amendment - ØØ and control with mineral fertilizer ØØ+CAN, 0.3g CAN/pot). Treatments included
soil (3 kg/pot) and biomass ash (30 g/pot) without and with one of three selected bacterial inoculums.
Bacterial strains Streptomyces fulvissimus CKS7, Hymenobacter sp. CKS3 and Sinorhizobium (Ensifer)
meliloti 207 were used for three inoculums preparation (CKS7, CKS7+CKS3 and 207). The ash and
experimental soil samples, as well as soil and plant samples collected at the stage of crop maturity
were tested for: total N, C, S, plant available P2O5 and K2O and for the content of potentially toxic and
hazardous microelements (As, Cd, Сo, Cr, Cu, Mn, Ni, Pb, Zn). The results showed that the addition of
biomass ash significantly reduced soil acidity and increased content of plant available P and K
compared to the controls (in all treatments). Barley yield was almost twice as higher in ash treatment
and by 1.56, 1.70 and 1.81 times higher in treatments Ash+CKS7, Ash+CKS7+CKS3 and Ash+207
respectively, compared to control (ØØ). The content of potentially toxic microelements was below the
maximum allowed concentrations in the soils and shoots. The use of combinations of ash and bacterial
inoculum improved the quality of barley in terms of nitrogen content. This study showed that biomass
ash can be used as fertilizer on acidic soils with low nutrient content alone or in combination with
applied inoculants and provide an environmentally friendly approach in agriculture to reduce the
need for chemical fertilizers and solve the problem of biomass ash disposal.",
publisher = "Federation of Eurasian Soil Science Societies",
journal = "The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey",
title = "The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality",
pages = "22",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6426"
}

Buntić, A., Tošić Jojević, S., Knežević, M., Milić, M., Mihajlovski, K., Koković, N.,& Saljnikov, E.. (2021). The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality. in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey
Federation of Eurasian Soil Science Societies., 22.
https://hdl.handle.net/21.15107/rcub_technorep_6426

Buntić A, Tošić Jojević S, Knežević M, Milić M, Mihajlovski K, Koković N, Saljnikov E. The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality. in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey. 2021;:22.
https://hdl.handle.net/21.15107/rcub_technorep_6426 .

Buntić, Aneta, Tošić Jojević, Sonja, Knežević, Magdalena, Milić, Marija, Mihajlovski, Katarina, Koković, Nikola, Saljnikov, Elmira, "The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality" in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey (2021):22,
https://hdl.handle.net/21.15107/rcub_technorep_6426 .

TY  - CONF
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera
AU  - Milić, Marija
AU  - Dubljanin, Tijana
AU  - Kuzmanović, Đorđe
AU  - Knežević, Magdalena
AU  - Delić, Dušica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6415
AB  - Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences
C3  - 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection
T1  - Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti
EP  - 163
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6415
ER  - 

@conference{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Dubljanin, Tijana and Kuzmanović, Đorđe and Knežević, Magdalena and Delić, Dušica",
year = "2019",
abstract = "Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences",
journal = "1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection",
title = "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti",
pages = "163-151",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6415"
}

Buntić, A., Stajković-Srbinović, O., Milić, M., Dubljanin, T., Kuzmanović, Đ., Knežević, M.,& Delić, D.. (2019). Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences., 151-163.
https://hdl.handle.net/21.15107/rcub_technorep_6415

Buntić A, Stajković-Srbinović O, Milić M, Dubljanin T, Kuzmanović Đ, Knežević M, Delić D. Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection. 2019;:151-163.
https://hdl.handle.net/21.15107/rcub_technorep_6415 .

Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Dubljanin, Tijana, Kuzmanović, Đorđe, Knežević, Magdalena, Delić, Dušica, "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti" in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection (2019):151-163,
https://hdl.handle.net/21.15107/rcub_technorep_6415 .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera S.
AU  - Knežević, Magdalena M.
AU  - Rasulić, Nataša V.
AU  - Kuzmanović, Đorđe Ž.
AU  - Dimitrijević-Branković, Suzana
AU  - Delić, Dušica I.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4291
AB  - Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley.
AB  - Zemljišne bakterije iz rizosfere korena biljaka najčešće imaju širok spektar pozitivnog dejstva na biljke I mogu poboljšati njhiov rast. Vrste roda Bacillus jedne su od najistraživanijih bakterija koje mogu doprinositi poboljšanju rasta biljaka. U ovom radu je izolovano sedam Bacillus izolata iz rizosfere lucerke (Medicago sativa). Ispitivana je sposobnost produkcije jedinjenja kao što su vodonik-cijanid, indol-3-sirćetna kiselina I siderofore. Procenjena je I sposobnost indukcije klijavosti na semena pšenice (Triticum aestivum) I ječma (Hordeum jubatum). Izolat BMG2 je proizveo najviše indol-3-sirćetne kiseline (28.89 µg/mL). Dužina korenčića je povećana za 60%, dok je dužina izdanka uvećana 2.23 puta primenom izolata PAZE-6 i BMG1. Pored toga, izolati BMG1, BMG2 i PAZE-6, su poboljšali klijavost obe vrste semena I pokazali sposobnost da proizvedu korisne supstance kao što su sideroforei indol-3sirćetna kiselina. Na dalje bi ovi izolati mogli da se koriste u proizvodnji tečnih aditiva koji bi povećali prinos kultivisanih biljaka, a posebno ječma.
PB  - Serbian Soil Science Society, Belgrade
T2  - Zemljište i biljka
T1  - The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination
T1  - Uticaj bakterijskih izolata iz zemljišta rizosfere na indukciju klijavosti semena pšenice i ječma
EP  - 11
IS  - 2
SP  - 1
VL  - 68
DO  - 10.5937/ZemBilj1902001B
ER  - 

@article{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Knežević, Magdalena M. and Rasulić, Nataša V. and Kuzmanović, Đorđe Ž. and Dimitrijević-Branković, Suzana and Delić, Dušica I.",
year = "2019",
abstract = "Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley., Zemljišne bakterije iz rizosfere korena biljaka najčešće imaju širok spektar pozitivnog dejstva na biljke I mogu poboljšati njhiov rast. Vrste roda Bacillus jedne su od najistraživanijih bakterija koje mogu doprinositi poboljšanju rasta biljaka. U ovom radu je izolovano sedam Bacillus izolata iz rizosfere lucerke (Medicago sativa). Ispitivana je sposobnost produkcije jedinjenja kao što su vodonik-cijanid, indol-3-sirćetna kiselina I siderofore. Procenjena je I sposobnost indukcije klijavosti na semena pšenice (Triticum aestivum) I ječma (Hordeum jubatum). Izolat BMG2 je proizveo najviše indol-3-sirćetne kiseline (28.89 µg/mL). Dužina korenčića je povećana za 60%, dok je dužina izdanka uvećana 2.23 puta primenom izolata PAZE-6 i BMG1. Pored toga, izolati BMG1, BMG2 i PAZE-6, su poboljšali klijavost obe vrste semena I pokazali sposobnost da proizvedu korisne supstance kao što su sideroforei indol-3sirćetna kiselina. Na dalje bi ovi izolati mogli da se koriste u proizvodnji tečnih aditiva koji bi povećali prinos kultivisanih biljaka, a posebno ječma.",
publisher = "Serbian Soil Science Society, Belgrade",
journal = "Zemljište i biljka",
title = "The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination, Uticaj bakterijskih izolata iz zemljišta rizosfere na indukciju klijavosti semena pšenice i ječma",
pages = "11-1",
number = "2",
volume = "68",
doi = "10.5937/ZemBilj1902001B"
}

Buntić, A., Stajković-Srbinović, O. S., Knežević, M. M., Rasulić, N. V., Kuzmanović, Đ. Ž., Dimitrijević-Branković, S.,& Delić, D. I.. (2019). The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination. in Zemljište i biljka
Serbian Soil Science Society, Belgrade., 68(2), 1-11.
https://doi.org/10.5937/ZemBilj1902001B

Buntić A, Stajković-Srbinović OS, Knežević MM, Rasulić NV, Kuzmanović ĐŽ, Dimitrijević-Branković S, Delić DI. The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination. in Zemljište i biljka. 2019;68(2):1-11.
doi:10.5937/ZemBilj1902001B .

Buntić, Aneta, Stajković-Srbinović, Olivera S., Knežević, Magdalena M., Rasulić, Nataša V., Kuzmanović, Đorđe Ž., Dimitrijević-Branković, Suzana, Delić, Dušica I., "The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination" in Zemljište i biljka, 68, no. 2 (2019):1-11,
https://doi.org/10.5937/ZemBilj1902001B . .