TY  - JOUR
AU  - Lužajić-Božinovski, Tijana
AU  - Todorovic, Vera
AU  - Milosevic, Ivan
AU  - Prokić, Bogomir Bolka
AU  - Gajdov, Vladimir
AU  - Nešović, Katarina
AU  - Mišković-Stanković, Vesna
AU  - Markovic, Danica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5021
AB  - Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in designing novel biomaterials that promote favorable macrophage-biomaterial surface interactions for clinical application. This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine, University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and immunohistochemical methods (CD68(+)). Statistical relevance was determined in the statistical software package SPSS 20 (IBM corp). The results of the study in terms of the number of giant cells localized around the implant showed that their number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the 30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and pericapsular space was highest in the group implanted with the commercial Suprasorb
T2  - Journal of Biomaterials Applications
T1  - Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats
EP  - 1125
IS  - 6
SP  - 1111
VL  - 36
DO  - 10.1177/08853282211046119
ER  - 

@article{
author = "Lužajić-Božinovski, Tijana and Todorovic, Vera and Milosevic, Ivan and Prokić, Bogomir Bolka and Gajdov, Vladimir and Nešović, Katarina and Mišković-Stanković, Vesna and Markovic, Danica",
year = "2022",
abstract = "Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in designing novel biomaterials that promote favorable macrophage-biomaterial surface interactions for clinical application. This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine, University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and immunohistochemical methods (CD68(+)). Statistical relevance was determined in the statistical software package SPSS 20 (IBM corp). The results of the study in terms of the number of giant cells localized around the implant showed that their number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the 30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and pericapsular space was highest in the group implanted with the commercial Suprasorb",
journal = "Journal of Biomaterials Applications",
title = "Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats",
pages = "1125-1111",
number = "6",
volume = "36",
doi = "10.1177/08853282211046119"
}

Lužajić-Božinovski, T., Todorovic, V., Milosevic, I., Prokić, B. B., Gajdov, V., Nešović, K., Mišković-Stanković, V.,& Markovic, D.. (2022). Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats. in Journal of Biomaterials Applications, 36(6), 1111-1125.
https://doi.org/10.1177/08853282211046119

Lužajić-Božinovski T, Todorovic V, Milosevic I, Prokić BB, Gajdov V, Nešović K, Mišković-Stanković V, Markovic D. Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats. in Journal of Biomaterials Applications. 2022;36(6):1111-1125.
doi:10.1177/08853282211046119 .

Lužajić-Božinovski, Tijana, Todorovic, Vera, Milosevic, Ivan, Prokić, Bogomir Bolka, Gajdov, Vladimir, Nešović, Katarina, Mišković-Stanković, Vesna, Markovic, Danica, "Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats" in Journal of Biomaterials Applications, 36, no. 6 (2022):1111-1125,
https://doi.org/10.1177/08853282211046119 . .

TY  - JOUR
AU  - Lužajić-Božinovski, Tijana
AU  - Todorovic, Vera
AU  - Milosevic, Ivan
AU  - Gajdov, Vladimir
AU  - Prokić, Bogomir Bolka
AU  - Nešović, Katarina
AU  - Mišković-Stanković, Vesna
AU  - Markovic, Danica
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4812
AB  - A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.
T2  - Acta Veterinaria-Beograd
T1  - Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model
EP  - 302
IS  - 3
SP  - 285
VL  - 71
DO  - 10.2478/acve-2021-0025
ER  - 

@article{
author = "Lužajić-Božinovski, Tijana and Todorovic, Vera and Milosevic, Ivan and Gajdov, Vladimir and Prokić, Bogomir Bolka and Nešović, Katarina and Mišković-Stanković, Vesna and Markovic, Danica",
year = "2021",
abstract = "A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model",
pages = "302-285",
number = "3",
volume = "71",
doi = "10.2478/acve-2021-0025"
}

Lužajić-Božinovski, T., Todorovic, V., Milosevic, I., Gajdov, V., Prokić, B. B., Nešović, K., Mišković-Stanković, V.,& Markovic, D.. (2021). Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd, 71(3), 285-302.
https://doi.org/10.2478/acve-2021-0025

Lužajić-Božinovski T, Todorovic V, Milosevic I, Gajdov V, Prokić BB, Nešović K, Mišković-Stanković V, Markovic D. Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd. 2021;71(3):285-302.
doi:10.2478/acve-2021-0025 .

Lužajić-Božinovski, Tijana, Todorovic, Vera, Milosevic, Ivan, Gajdov, Vladimir, Prokić, Bogomir Bolka, Nešović, Katarina, Mišković-Stanković, Vesna, Markovic, Danica, "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model" in Acta Veterinaria-Beograd, 71, no. 3 (2021):285-302,
https://doi.org/10.2478/acve-2021-0025 . .