TY  - JOUR
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić Bujagić, Ivana
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7260
AB  - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
PB  - Society of Chemists and Technologists of Macedonia
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications
EP  - 262
IS  - 2
SP  - 249
VL  - 42
DO  - 10.20450/mjcce.2023.2775
ER  - 

@article{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE-SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) after immersion in simulated body fluid (SBF) at 37 °C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect” with ~ 30 % of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureus TL and Escherich-ia coli ATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.",
publisher = "Society of Chemists and Technologists of Macedonia",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications",
pages = "262-249",
number = "2",
volume = "42",
doi = "10.20450/mjcce.2023.2775"
}

Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering
Society of Chemists and Technologists of Macedonia., 42(2), 249-262.
https://doi.org/10.20450/mjcce.2023.2775

Jaćimović N, Đošić M, Janković A, Grujić S, Matić Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262.
doi:10.20450/mjcce.2023.2775 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262,
https://doi.org/10.20450/mjcce.2023.2775 . .

TY  - CONF
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5029
AB  - Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), X ray diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue.
PB  - Institute of Technical Sciences of SASA
C3  - Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
T1  - Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin
SP  - 7
VL  - 18
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5029
ER  - 

@conference{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), X ray diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts",
title = "Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin",
pages = "7",
volume = "18",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5029"
}

Jaćimović, N., Đošić, M., Janković, A.,& Mišković-Stanković, V.. (2021). Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts
Institute of Technical Sciences of SASA., 18, 7.
https://hdl.handle.net/21.15107/rcub_technorep_5029

Jaćimović N, Đošić M, Janković A, Mišković-Stanković V. Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts. 2021;18:7.
https://hdl.handle.net/21.15107/rcub_technorep_5029 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin" in Nineteenth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts, 18 (2021):7,
https://hdl.handle.net/21.15107/rcub_technorep_5029 .