@conference{
author = "Mišković-Stanković, Vesna and Nešović, Katarina and Janković, Ana and Radetić, Tamara and Perić-Grujić, Aleksandra and Vukašinović-Sekulić, Maja and Kojić, Vesna and Yop Rhee, Kyong",
year = "2019",
abstract = "In recent times, polymer-based hydrogel materials have presented themselves as excellent candidates for
new-generation wound dressings with improved properties, such as high sorption ability, good mechanical
properties and low adhesiveness [1]. Additionally, cross linked hydrogel matrices serve as excellent carriers
for controlled release of antibacterial agents, such as silver nanoparticles (Ag/NPs), which are preferred over
conventional antibiotics due to multi-phase mechanism of action and low susceptibility to induce bacterial
resistance [2]. In this work, we aim to produce novel silver/poly(vinyl alcohol)/chitosan (Ag/PVA/CHI)
hydrogels for wound dressing applications. The electrochemical route for AgNPs synthesis provided facile
and green method for the reduction of Ag+
ions inside the hydrogel matrices, without the need to use toxic
chemical reducing agents [3]. The effect of chitosan content on the synthesis yield, antibacterial properties,
swelling and release kinetics was investigated. The formation of AgNPs was confirmed using UV-visible
spectroscopy through the appearance of plasmon resonant peaks at around 400 nm (Figure 1a), whereas
transmission electron microscopy (TEM) proved the incorporation of both single and polycrystalline spherical
AgNPs with diameters of 5-10 nm (Figure 1b). The swelling and silver release kinetics were investigated in
modified phosphate buffer solutions (pH 7.4) at 37 °C to mimic physiological conditions. The obtained
swelling isotherms and release profiles were fitted with different models to obtain kinetic and diffusion
parameters (Figure 1c). The antibacterial activity was evaluated against Staphylococcus aureus TL and
Escherichia coli ATCC 25922 bacterial strains using an in-suspension test, and non-toxicity of both silvercontaining and silver-free hydrogels was proved by MTT cytotoxicity test. The obtained results confirmed
strong potential of Ag/PVA/CHI hydrogels for biomedical applications.",
publisher = "International Association of Physical Chemists",
journal = "Book of abstracts / 7th Regional Symposium on Electrochemistry – South East Europe & 8th Kurt Schwabe Symposium, Split, Croatia, May 27-30, 2019",
title = "Poly(vinyl alcohol)/chitosan hydrogels with electrochemically synthesized silver nanoparticles for wound dressing applications",
pages = "121",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7245"
}
