TY  - JOUR
AU  - Đurđić, Slađana
AU  - Mišković-Stanković, Vesna
AU  - Ražić, Slavica
AU  - Mutić, Jelena
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4416
AB  - The analysis of isotope ratios of lead in the mushrooms and soil, where they were grown, assisted with a principal component analysis, offered a new perspective for understanding possible chemical environment in a real setup of those compartments. The content of lead and its isotope compositions were determined in soil samples and mushroom Macrolepiota procera from unpolluted area of Mountain Goč, Serbia. Sequential extraction procedure based on the Commission of the European Community Bureau of Reference (BCR) was applied on soil samples in order to determine the distribution of lead in the labile and un-labile fractions of the soil. Caps and stipes of mushrooms were subjected to microwave acid-assisted digestion prior to measurements by inductively coupled plasma quadrupole mass spectrometer for determination of lead content and lead isotope ratios. Information about the chemical fractionation of Pb in soil, Pb isotopic data from soil fractions and fruiting bodies allowed a more detailed insight on the uptake mechanisms. Lead was predominantly associated with reducible fraction (~ 60%). Only its small portion (∼ 1%) was present in the exchangeable and acid-extractable fractions suggesting the low mobility of Pb. Lead isotope analysis revealed the presence of anthropogenic lead in the surface soil. Significant lower 206Pb/207Pb compared with other fractions was found in exchangeable and acid-soluble fraction (1.331 ± 0.010), which corresponds to the isotope ratio of European gasoline. The highest 206Pb/207Pb ratio was observed in reducible fraction (1.162 ± 0.007), while in oxidizable and residual fraction, those values were similar (1.159 ± 0.006 and 1.159 ± 0.004, respectively). Distinction of exchangeable and acid-extractable fractions from others was also confirmed, for the first time, by principal component analysis. The analysis of four isotope ratios (206Pb/207Pb, 208Pb/206Pb, 206Pb/204Pb, and 207Pb/204Pb) indicated that the analyzed M. procera accumulates lead from the first two fractions of topsoil layers.
PB  - Springer Nature
T2  - Environmental Science and Pollution Research
T1  - Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil
DO  - 10.1007/s11356-020-07947-6
ER  - 

@article{
author = "Đurđić, Slađana and Mišković-Stanković, Vesna and Ražić, Slavica and Mutić, Jelena",
year = "2020",
abstract = "The analysis of isotope ratios of lead in the mushrooms and soil, where they were grown, assisted with a principal component analysis, offered a new perspective for understanding possible chemical environment in a real setup of those compartments. The content of lead and its isotope compositions were determined in soil samples and mushroom Macrolepiota procera from unpolluted area of Mountain Goč, Serbia. Sequential extraction procedure based on the Commission of the European Community Bureau of Reference (BCR) was applied on soil samples in order to determine the distribution of lead in the labile and un-labile fractions of the soil. Caps and stipes of mushrooms were subjected to microwave acid-assisted digestion prior to measurements by inductively coupled plasma quadrupole mass spectrometer for determination of lead content and lead isotope ratios. Information about the chemical fractionation of Pb in soil, Pb isotopic data from soil fractions and fruiting bodies allowed a more detailed insight on the uptake mechanisms. Lead was predominantly associated with reducible fraction (~ 60%). Only its small portion (∼ 1%) was present in the exchangeable and acid-extractable fractions suggesting the low mobility of Pb. Lead isotope analysis revealed the presence of anthropogenic lead in the surface soil. Significant lower 206Pb/207Pb compared with other fractions was found in exchangeable and acid-soluble fraction (1.331 ± 0.010), which corresponds to the isotope ratio of European gasoline. The highest 206Pb/207Pb ratio was observed in reducible fraction (1.162 ± 0.007), while in oxidizable and residual fraction, those values were similar (1.159 ± 0.006 and 1.159 ± 0.004, respectively). Distinction of exchangeable and acid-extractable fractions from others was also confirmed, for the first time, by principal component analysis. The analysis of four isotope ratios (206Pb/207Pb, 208Pb/206Pb, 206Pb/204Pb, and 207Pb/204Pb) indicated that the analyzed M. procera accumulates lead from the first two fractions of topsoil layers.",
publisher = "Springer Nature",
journal = "Environmental Science and Pollution Research",
title = "Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil",
doi = "10.1007/s11356-020-07947-6"
}

Đurđić, S., Mišković-Stanković, V., Ražić, S.,& Mutić, J.. (2020). Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil. in Environmental Science and Pollution Research
Springer Nature..
https://doi.org/10.1007/s11356-020-07947-6

Đurđić S, Mišković-Stanković V, Ražić S, Mutić J. Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil. in Environmental Science and Pollution Research. 2020;.
doi:10.1007/s11356-020-07947-6 .

Đurđić, Slađana, Mišković-Stanković, Vesna, Ražić, Slavica, Mutić, Jelena, "Lead isotope ratios as tool for elucidation of chemical environment in a system of Macrolepiota procera (Scop.) Singer - soil" in Environmental Science and Pollution Research (2020),
https://doi.org/10.1007/s11356-020-07947-6 . .

TY  - JOUR
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Apostolović, Danijela
AU  - Cvetković, Anka
AU  - Veljović, Đorđe
AU  - Mutić, Jelena
AU  - van Hage, Marianne
AU  - Burazer, Lidija M.
AU  - Ćirković-Veličković, Tanja
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4169
AB  - An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide the most relevant assessment of the effects of the intensity and diversity of urban and industrial contamination on pollen structure and allergenicity. The significance of in-depth post-translational modification (PTM) studies of pollen proteomes, when compared with studies on other aspects of pollution and altered pollen allergenicity, has not yet been determined; hence, little progress has been made within this field. We undertook a comprehensive comparative analysis of multiple polluted and environmentally preserved Phleum pratense (Timothy grass) pollen samples using scanning electron microscopy, in-depth PTM profiling, determination of organic and inorganic pollutants, analysis of the release of sub-pollen particles and phenols/proteins, and analysis of proteome expression using high resolution tandem mass spectrometry. In addition, we used quantitative enzyme-linked immunosorbent assays (ELISA) and immunoglobulin E (IgE) immunoblotting. An increased phenolic content and release of sub-pollen particles was found in pollen samples from the polluted area, including a significantly higher content of mercury, cadmium, and manganese, with irregular long spines on pollen grain surface structures. Antioxidative defense-related enzymes were significantly upregulated and seven oxidative PTMs were significantly increased (methionine, histidine, lysine, and proline oxidation; tyrosine glycosylation, lysine 4-hydroxy-2-nonenal adduct, and lysine carbamylation) in pollen exposed to the chemical plant and road traffic pollution sources. Oxidative modifications affected several Timothy pollen allergens; Phl p 6, in particular, exhibited several different oxidative modifications. The expression of Phl p 6, 12, and 13 allergens were downregulated in polluted pollen, and IgE binding to pollen extract was substantially lower in the 18 patients studied, as measured by quantitative ELISA. Quantitative, unrestricted, and detailed PTM searches using an enrichment-free approach pointed to modification of Timothy pollen allergens and suggested that heavy metals are primarily responsible for oxidative stress effects observed in pollen proteins.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environment International
T1  - In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress
EP  - 658
SP  - 644
VL  - 126
DO  - 10.1016/j.envint.2019.03.001
ER  - 

@article{
author = "Smiljanić, Katarina and Prodić, Ivana and Apostolović, Danijela and Cvetković, Anka and Veljović, Đorđe and Mutić, Jelena and van Hage, Marianne and Burazer, Lidija M. and Ćirković-Veličković, Tanja",
year = "2019",
abstract = "An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide the most relevant assessment of the effects of the intensity and diversity of urban and industrial contamination on pollen structure and allergenicity. The significance of in-depth post-translational modification (PTM) studies of pollen proteomes, when compared with studies on other aspects of pollution and altered pollen allergenicity, has not yet been determined; hence, little progress has been made within this field. We undertook a comprehensive comparative analysis of multiple polluted and environmentally preserved Phleum pratense (Timothy grass) pollen samples using scanning electron microscopy, in-depth PTM profiling, determination of organic and inorganic pollutants, analysis of the release of sub-pollen particles and phenols/proteins, and analysis of proteome expression using high resolution tandem mass spectrometry. In addition, we used quantitative enzyme-linked immunosorbent assays (ELISA) and immunoglobulin E (IgE) immunoblotting. An increased phenolic content and release of sub-pollen particles was found in pollen samples from the polluted area, including a significantly higher content of mercury, cadmium, and manganese, with irregular long spines on pollen grain surface structures. Antioxidative defense-related enzymes were significantly upregulated and seven oxidative PTMs were significantly increased (methionine, histidine, lysine, and proline oxidation; tyrosine glycosylation, lysine 4-hydroxy-2-nonenal adduct, and lysine carbamylation) in pollen exposed to the chemical plant and road traffic pollution sources. Oxidative modifications affected several Timothy pollen allergens; Phl p 6, in particular, exhibited several different oxidative modifications. The expression of Phl p 6, 12, and 13 allergens were downregulated in polluted pollen, and IgE binding to pollen extract was substantially lower in the 18 patients studied, as measured by quantitative ELISA. Quantitative, unrestricted, and detailed PTM searches using an enrichment-free approach pointed to modification of Timothy pollen allergens and suggested that heavy metals are primarily responsible for oxidative stress effects observed in pollen proteins.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environment International",
title = "In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress",
pages = "658-644",
volume = "126",
doi = "10.1016/j.envint.2019.03.001"
}

Smiljanić, K., Prodić, I., Apostolović, D., Cvetković, A., Veljović, Đ., Mutić, J., van Hage, M., Burazer, L. M.,& Ćirković-Veličković, T.. (2019). In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress. in Environment International
Pergamon-Elsevier Science Ltd, Oxford., 126, 644-658.
https://doi.org/10.1016/j.envint.2019.03.001

Smiljanić K, Prodić I, Apostolović D, Cvetković A, Veljović Đ, Mutić J, van Hage M, Burazer LM, Ćirković-Veličković T. In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress. in Environment International. 2019;126:644-658.
doi:10.1016/j.envint.2019.03.001 .

Smiljanić, Katarina, Prodić, Ivana, Apostolović, Danijela, Cvetković, Anka, Veljović, Đorđe, Mutić, Jelena, van Hage, Marianne, Burazer, Lidija M., Ćirković-Veličković, Tanja, "In-depth quantitative profiling of post-translational modifications of Timothy grass pollen allergome in relation to environmental oxidative stress" in Environment International, 126 (2019):644-658,
https://doi.org/10.1016/j.envint.2019.03.001 . .

TY  - JOUR
AU  - Barać, Nemanja
AU  - Škrivanj, Sandra B.
AU  - Mutić, Jelena
AU  - Manojlović, Dragan D.
AU  - Bukumirić, Zoran
AU  - Živojinović, Dragana
AU  - Petrović, Rada
AU  - Ćorac, Aleksandar
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3311
AB  - Improved understanding of the relationships between heavy metals fractionation in agricultural soils and biological uptake could be obtained by analysing samples of biota in parallel with sequential extraction of their grown media. The overall goals of this study were to identify the characteristics of metal fractions and their bioavailability to maize and potato plants in the agricultural land of the Ibar River in southern Serbia and northern Kosovo. The concentrations of Pb, Zn, Cd, Ni, Cr and Cu in soil and vegetable samples were determined by the ICP-OES method. Pb/Zn production and industrial waste disposal significantly increased the pseudo-total concentrations of heavy metals in the soil together with their mobile and potentially bioavailable amounts. The Pb concentrations in the vegetable samples were generally above the EU maximum permitted concentrations in foodstuffs. However, the concentrations of Zn, Ni, Cr and Cu in the vegetables were below the critical levels. The results of the present study indicated that the intensive industrial production of Pb/Zn over the years and permanent pollution were responsible for the environmental contamination by heavy metals in the study area, particularly by Pb and Zn. The quantity of the mobile and potentially bioavailable heavy metals in the studied soils threatens the quality of Zea mays L. and Solanum tuberosum L. crops, with a real risk that these elements could enter the food chain.
PB  - Springer International Publishing Ag, Cham
T2  - Water Air and Soil Pollution
T1  - Heavy Metals Fractionation in Agricultural Soils of Pb/Zn Mining Region and Their Transfer to Selected Vegetables
IS  - 12
VL  - 227
DO  - 10.1007/s11270-016-3177-4
ER  - 

@article{
author = "Barać, Nemanja and Škrivanj, Sandra B. and Mutić, Jelena and Manojlović, Dragan D. and Bukumirić, Zoran and Živojinović, Dragana and Petrović, Rada and Ćorac, Aleksandar",
year = "2016",
abstract = "Improved understanding of the relationships between heavy metals fractionation in agricultural soils and biological uptake could be obtained by analysing samples of biota in parallel with sequential extraction of their grown media. The overall goals of this study were to identify the characteristics of metal fractions and their bioavailability to maize and potato plants in the agricultural land of the Ibar River in southern Serbia and northern Kosovo. The concentrations of Pb, Zn, Cd, Ni, Cr and Cu in soil and vegetable samples were determined by the ICP-OES method. Pb/Zn production and industrial waste disposal significantly increased the pseudo-total concentrations of heavy metals in the soil together with their mobile and potentially bioavailable amounts. The Pb concentrations in the vegetable samples were generally above the EU maximum permitted concentrations in foodstuffs. However, the concentrations of Zn, Ni, Cr and Cu in the vegetables were below the critical levels. The results of the present study indicated that the intensive industrial production of Pb/Zn over the years and permanent pollution were responsible for the environmental contamination by heavy metals in the study area, particularly by Pb and Zn. The quantity of the mobile and potentially bioavailable heavy metals in the studied soils threatens the quality of Zea mays L. and Solanum tuberosum L. crops, with a real risk that these elements could enter the food chain.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Water Air and Soil Pollution",
title = "Heavy Metals Fractionation in Agricultural Soils of Pb/Zn Mining Region and Their Transfer to Selected Vegetables",
number = "12",
volume = "227",
doi = "10.1007/s11270-016-3177-4"
}

Barać, N., Škrivanj, S. B., Mutić, J., Manojlović, D. D., Bukumirić, Z., Živojinović, D., Petrović, R.,& Ćorac, A.. (2016). Heavy Metals Fractionation in Agricultural Soils of Pb/Zn Mining Region and Their Transfer to Selected Vegetables. in Water Air and Soil Pollution
Springer International Publishing Ag, Cham., 227(12).
https://doi.org/10.1007/s11270-016-3177-4

Barać N, Škrivanj SB, Mutić J, Manojlović DD, Bukumirić Z, Živojinović D, Petrović R, Ćorac A. Heavy Metals Fractionation in Agricultural Soils of Pb/Zn Mining Region and Their Transfer to Selected Vegetables. in Water Air and Soil Pollution. 2016;227(12).
doi:10.1007/s11270-016-3177-4 .

Barać, Nemanja, Škrivanj, Sandra B., Mutić, Jelena, Manojlović, Dragan D., Bukumirić, Zoran, Živojinović, Dragana, Petrović, Rada, Ćorac, Aleksandar, "Heavy Metals Fractionation in Agricultural Soils of Pb/Zn Mining Region and Their Transfer to Selected Vegetables" in Water Air and Soil Pollution, 227, no. 12 (2016),
https://doi.org/10.1007/s11270-016-3177-4 . .