TY  - JOUR
AU  - Lužajić-Božinovski, Tijana
AU  - Todorovic, Vera
AU  - Milosevic, Ivan
AU  - Prokić, Bogomir Bolka
AU  - Gajdov, Vladimir
AU  - Nešović, Katarina
AU  - Mišković-Stanković, Vesna
AU  - Markovic, Danica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5021
AB  - Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in designing novel biomaterials that promote favorable macrophage-biomaterial surface interactions for clinical application. This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine, University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and immunohistochemical methods (CD68(+)). Statistical relevance was determined in the statistical software package SPSS 20 (IBM corp). The results of the study in terms of the number of giant cells localized around the implant showed that their number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the 30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and pericapsular space was highest in the group implanted with the commercial Suprasorb
T2  - Journal of Biomaterials Applications
T1  - Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats
EP  - 1125
IS  - 6
SP  - 1111
VL  - 36
DO  - 10.1177/08853282211046119
ER  - 

@article{
author = "Lužajić-Božinovski, Tijana and Todorovic, Vera and Milosevic, Ivan and Prokić, Bogomir Bolka and Gajdov, Vladimir and Nešović, Katarina and Mišković-Stanković, Vesna and Markovic, Danica",
year = "2022",
abstract = "Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in designing novel biomaterials that promote favorable macrophage-biomaterial surface interactions for clinical application. This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine, University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and immunohistochemical methods (CD68(+)). Statistical relevance was determined in the statistical software package SPSS 20 (IBM corp). The results of the study in terms of the number of giant cells localized around the implant showed that their number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the 30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and pericapsular space was highest in the group implanted with the commercial Suprasorb",
journal = "Journal of Biomaterials Applications",
title = "Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats",
pages = "1125-1111",
number = "6",
volume = "36",
doi = "10.1177/08853282211046119"
}

Lužajić-Božinovski, T., Todorovic, V., Milosevic, I., Prokić, B. B., Gajdov, V., Nešović, K., Mišković-Stanković, V.,& Markovic, D.. (2022). Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats. in Journal of Biomaterials Applications, 36(6), 1111-1125.
https://doi.org/10.1177/08853282211046119

Lužajić-Božinovski T, Todorovic V, Milosevic I, Prokić BB, Gajdov V, Nešović K, Mišković-Stanković V, Markovic D. Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats. in Journal of Biomaterials Applications. 2022;36(6):1111-1125.
doi:10.1177/08853282211046119 .

Lužajić-Božinovski, Tijana, Todorovic, Vera, Milosevic, Ivan, Prokić, Bogomir Bolka, Gajdov, Vladimir, Nešović, Katarina, Mišković-Stanković, Vesna, Markovic, Danica, "Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats" in Journal of Biomaterials Applications, 36, no. 6 (2022):1111-1125,
https://doi.org/10.1177/08853282211046119 . .

TY  - JOUR
AU  - Lužajić-Božinovski, Tijana
AU  - Todorovic, Vera
AU  - Milosevic, Ivan
AU  - Gajdov, Vladimir
AU  - Prokić, Bogomir Bolka
AU  - Nešović, Katarina
AU  - Mišković-Stanković, Vesna
AU  - Markovic, Danica
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4812
AB  - A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.
T2  - Acta Veterinaria-Beograd
T1  - Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model
EP  - 302
IS  - 3
SP  - 285
VL  - 71
DO  - 10.2478/acve-2021-0025
ER  - 

@article{
author = "Lužajić-Božinovski, Tijana and Todorovic, Vera and Milosevic, Ivan and Gajdov, Vladimir and Prokić, Bogomir Bolka and Nešović, Katarina and Mišković-Stanković, Vesna and Markovic, Danica",
year = "2021",
abstract = "A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials - silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (alpha-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model",
pages = "302-285",
number = "3",
volume = "71",
doi = "10.2478/acve-2021-0025"
}

Lužajić-Božinovski, T., Todorovic, V., Milosevic, I., Gajdov, V., Prokić, B. B., Nešović, K., Mišković-Stanković, V.,& Markovic, D.. (2021). Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd, 71(3), 285-302.
https://doi.org/10.2478/acve-2021-0025

Lužajić-Božinovski T, Todorovic V, Milosevic I, Gajdov V, Prokić BB, Nešović K, Mišković-Stanković V, Markovic D. Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd. 2021;71(3):285-302.
doi:10.2478/acve-2021-0025 .

Lužajić-Božinovski, Tijana, Todorovic, Vera, Milosevic, Ivan, Gajdov, Vladimir, Prokić, Bogomir Bolka, Nešović, Katarina, Mišković-Stanković, Vesna, Markovic, Danica, "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model" in Acta Veterinaria-Beograd, 71, no. 3 (2021):285-302,
https://doi.org/10.2478/acve-2021-0025 . .

TY  - JOUR
AU  - Lužajić-Božinovski, Tijana
AU  - Marković, Danica
AU  - Todorović, Vera
AU  - Prokić, Bogomir Bolka
AU  - Milošević, Ivan
AU  - Drndarević, Neda
AU  - Nešović, Katarina
AU  - Kyong, Yop Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3924
AB  - In this paper, we have shown for the first time the soft tissue response of novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria, Beograd
T1  - In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience
EP  - 339
IS  - 3
SP  - 321
VL  - 68
DO  - 10.2478/acve-2018-0027
ER  - 

@article{
author = "Lužajić-Božinovski, Tijana and Marković, Danica and Todorović, Vera and Prokić, Bogomir Bolka and Milošević, Ivan and Drndarević, Neda and Nešović, Katarina and Kyong, Yop Rhee and Mišković-Stanković, Vesna",
year = "2018",
abstract = "In this paper, we have shown for the first time the soft tissue response of novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria, Beograd",
title = "In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience",
pages = "339-321",
number = "3",
volume = "68",
doi = "10.2478/acve-2018-0027"
}

Lužajić-Božinovski, T., Marković, D., Todorović, V., Prokić, B. B., Milošević, I., Drndarević, N., Nešović, K., Kyong, Y. R.,& Mišković-Stanković, V.. (2018). In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience. in Acta Veterinaria, Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 321-339.
https://doi.org/10.2478/acve-2018-0027

Lužajić-Božinovski T, Marković D, Todorović V, Prokić BB, Milošević I, Drndarević N, Nešović K, Kyong YR, Mišković-Stanković V. In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience. in Acta Veterinaria, Beograd. 2018;68(3):321-339.
doi:10.2478/acve-2018-0027 .

Lužajić-Božinovski, Tijana, Marković, Danica, Todorović, Vera, Prokić, Bogomir Bolka, Milošević, Ivan, Drndarević, Neda, Nešović, Katarina, Kyong, Yop Rhee, Mišković-Stanković, Vesna, "In vivo investigation of soft tissue response of novel silver/poly(vinyl alcohol)/ graphene and silver/poly(vinyl alcohol)/chitosan/graphene hydrogels aimed for medical applications - the first experience" in Acta Veterinaria, Beograd, 68, no. 3 (2018):321-339,
https://doi.org/10.2478/acve-2018-0027 . .

TY  - JOUR
AU  - Prokić, Bogomir Bolka
AU  - Bačić, G.
AU  - Prokić, B.
AU  - Kalijadis, Ana
AU  - Todorović, Vera
AU  - Puškaš, Nela
AU  - Vidojević, D.
AU  - Laušević, Mila
AU  - Laušević, Z.
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5692
AB  - In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic) with muscle and subcutaneous tissues of rabbits. MRI techniques showed to be useful in longitudinal monitoring of the surrounding tissues, assessment of biocompatibility of new implants, and in the distinction of in vivo surgical edema from inflammation. Histopathology confirmed MRI results, thus showing that MRI has a great potential for in vivo studies of such materials.
AB  - U modernoj medicine implanti su veoma važni, kao i njihovo dizajniranje i izbor materijala. Isto tako je značajan i izbor tehnike kojom se prati in vivo njihova sudbina i biokompatibilnost. Cilj ove studije je bila procena mogućnosti praćenja biokompatibilnosti dva novo dizajnirana karbonska vlakna pomoću magnetic resonance imaging (MRI). Analizirali smo interakciju karbonskih vlakana sa funkcionalizovanom površinom (kiselinom i bazom) sa mišićnim i potkožnim tkivom kunića. MRI tehnika se pokazala uspešnom u toku praćenja reakcije okolnog tkiva, i procene biokompatibilnosti nove vrste implanata, jer je uspela da in vivo odvoji hirurški edem od inflamacije. Patohistološka analiza je potvrdila rezultate MRI pokazujući time da MRI ima veliki potencijal za in vivo studije takvih materijala.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues
T1  - In vivo MRI procena biokompatibilnosti funkcionalizovanih karbonskih vlakana u reakciji sa mekim tkivom
EP  - 696
IS  - 5-6
SP  - 683
VL  - 62
DO  - 10.2298/AVB1206683P
ER  - 

@article{
author = "Prokić, Bogomir Bolka and Bačić, G. and Prokić, B. and Kalijadis, Ana and Todorović, Vera and Puškaš, Nela and Vidojević, D. and Laušević, Mila and Laušević, Z.",
year = "2012",
abstract = "In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic) with muscle and subcutaneous tissues of rabbits. MRI techniques showed to be useful in longitudinal monitoring of the surrounding tissues, assessment of biocompatibility of new implants, and in the distinction of in vivo surgical edema from inflammation. Histopathology confirmed MRI results, thus showing that MRI has a great potential for in vivo studies of such materials., U modernoj medicine implanti su veoma važni, kao i njihovo dizajniranje i izbor materijala. Isto tako je značajan i izbor tehnike kojom se prati in vivo njihova sudbina i biokompatibilnost. Cilj ove studije je bila procena mogućnosti praćenja biokompatibilnosti dva novo dizajnirana karbonska vlakna pomoću magnetic resonance imaging (MRI). Analizirali smo interakciju karbonskih vlakana sa funkcionalizovanom površinom (kiselinom i bazom) sa mišićnim i potkožnim tkivom kunića. MRI tehnika se pokazala uspešnom u toku praćenja reakcije okolnog tkiva, i procene biokompatibilnosti nove vrste implanata, jer je uspela da in vivo odvoji hirurški edem od inflamacije. Patohistološka analiza je potvrdila rezultate MRI pokazujući time da MRI ima veliki potencijal za in vivo studije takvih materijala.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues, In vivo MRI procena biokompatibilnosti funkcionalizovanih karbonskih vlakana u reakciji sa mekim tkivom",
pages = "696-683",
number = "5-6",
volume = "62",
doi = "10.2298/AVB1206683P"
}

Prokić, B. B., Bačić, G., Prokić, B., Kalijadis, A., Todorović, V., Puškaš, N., Vidojević, D., Laušević, M.,& Laušević, Z.. (2012). In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 683-696.
https://doi.org/10.2298/AVB1206683P

Prokić BB, Bačić G, Prokić B, Kalijadis A, Todorović V, Puškaš N, Vidojević D, Laušević M, Laušević Z. In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues. in Acta Veterinaria-Beograd. 2012;62(5-6):683-696.
doi:10.2298/AVB1206683P .

Prokić, Bogomir Bolka, Bačić, G., Prokić, B., Kalijadis, Ana, Todorović, Vera, Puškaš, Nela, Vidojević, D., Laušević, Mila, Laušević, Z., "In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):683-696,
https://doi.org/10.2298/AVB1206683P . .