TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Momić, Tatjana
AU  - Onjia, Antonije
AU  - Vujisić, Ljubodrag V.
AU  - Vasić, Vesna M.
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1639
AB  - In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
PB  - Springer Wien, Wien
T2  - Microchimica Acta
T1  - Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods
EP  - 297
IS  - 3-4
SP  - 289
VL  - 170
DO  - 10.1007/s00604-010-0324-2
ER  - 

@article{
author = "Lazarević-Pašti, Tamara and Momić, Tatjana and Onjia, Antonije and Vujisić, Ljubodrag V. and Vasić, Vesna M.",
year = "2010",
abstract = "In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.",
publisher = "Springer Wien, Wien",
journal = "Microchimica Acta",
title = "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods",
pages = "297-289",
number = "3-4",
volume = "170",
doi = "10.1007/s00604-010-0324-2"
}

Lazarević-Pašti, T., Momić, T., Onjia, A., Vujisić, L. V.,& Vasić, V. M.. (2010). Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta
Springer Wien, Wien., 170(3-4), 289-297.
https://doi.org/10.1007/s00604-010-0324-2

Lazarević-Pašti T, Momić T, Onjia A, Vujisić LV, Vasić VM. Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta. 2010;170(3-4):289-297.
doi:10.1007/s00604-010-0324-2 .

Lazarević-Pašti, Tamara, Momić, Tatjana, Onjia, Antonije, Vujisić, Ljubodrag V., Vasić, Vesna M., "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods" in Microchimica Acta, 170, no. 3-4 (2010):289-297,
https://doi.org/10.1007/s00604-010-0324-2 . .