TY  - JOUR
AU  - Filipović, Vuk V.
AU  - Babić Radić, Marija M.
AU  - Vuković, Jovana S.
AU  - Vukomanović, Marija
AU  - Rubert, Marina
AU  - Hofmann, Sandra
AU  - Müller, Ralph
AU  - Tomić, Simonida Lj.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5018
AB  - New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the structure and the physicochemical and mechanical properties, and a natural polymer, to ensure the specific biological functions of the scaffold, with inorganic filler was applied. The goal was to make a new material with superior properties for applications in the biomedical field which mimics as closely as possible the native bone extracellular matrix (ECM). Biodegradable PHEMA hydrogel was obtained by crosslinking HEMA by poly(beta-amino esters) (PBAE). The scaffold's physicochemical and mechanical properties, in vitro degradation, and biological activity were assessed so to study the effects of the incorporation of nHAp in the (PHEMA/PBAE/gelatin) hydrogel, as well as the effect of the different pore-forming methods. Cryogels had higher elasticity, swelling, porosity, and percent of mass loss during degradation than the samples obtained by porogenation. The composite scaffolds had a higher mechanical strength, 10.14 MPa for the porogenated samples and 5.87 MPa for the cryogels, but a slightly lower degree of swelling, percent of mass loss, and porosity than the hybrid ones. All the scaffolds were nontoxic and had a high cell adhesion rate, which was 15-20% higher in the composite samples. Cell metabolic activity after 2 and 7 days of culture was higher in the composites, although not statistically different. After 28 days, cell metabolic activity was similar in all scaffolds and the TCP control. No effect of integrating nHAp into the scaffolds on osteogenic cell differentiation could be observed. Synergetic effects occurred which influenced the mechanical behavior, structure, physicochemical properties, and interactions with biological species.
T2  - Polymers
T1  - Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite
IS  - 1
SP  - 18
VL  - 14
DO  - 10.3390/polym14010018
ER  - 

@article{
author = "Filipović, Vuk V. and Babić Radić, Marija M. and Vuković, Jovana S. and Vukomanović, Marija and Rubert, Marina and Hofmann, Sandra and Müller, Ralph and Tomić, Simonida Lj.",
year = "2022",
abstract = "New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the structure and the physicochemical and mechanical properties, and a natural polymer, to ensure the specific biological functions of the scaffold, with inorganic filler was applied. The goal was to make a new material with superior properties for applications in the biomedical field which mimics as closely as possible the native bone extracellular matrix (ECM). Biodegradable PHEMA hydrogel was obtained by crosslinking HEMA by poly(beta-amino esters) (PBAE). The scaffold's physicochemical and mechanical properties, in vitro degradation, and biological activity were assessed so to study the effects of the incorporation of nHAp in the (PHEMA/PBAE/gelatin) hydrogel, as well as the effect of the different pore-forming methods. Cryogels had higher elasticity, swelling, porosity, and percent of mass loss during degradation than the samples obtained by porogenation. The composite scaffolds had a higher mechanical strength, 10.14 MPa for the porogenated samples and 5.87 MPa for the cryogels, but a slightly lower degree of swelling, percent of mass loss, and porosity than the hybrid ones. All the scaffolds were nontoxic and had a high cell adhesion rate, which was 15-20% higher in the composite samples. Cell metabolic activity after 2 and 7 days of culture was higher in the composites, although not statistically different. After 28 days, cell metabolic activity was similar in all scaffolds and the TCP control. No effect of integrating nHAp into the scaffolds on osteogenic cell differentiation could be observed. Synergetic effects occurred which influenced the mechanical behavior, structure, physicochemical properties, and interactions with biological species.",
journal = "Polymers",
title = "Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite",
number = "1",
pages = "18",
volume = "14",
doi = "10.3390/polym14010018"
}

Filipović, V. V., Babić Radić, M. M., Vuković, J. S., Vukomanović, M., Rubert, M., Hofmann, S., Müller, R.,& Tomić, S. Lj.. (2022). Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite. in Polymers, 14(1), 18.
https://doi.org/10.3390/polym14010018

Filipović VV, Babić Radić MM, Vuković JS, Vukomanović M, Rubert M, Hofmann S, Müller R, Tomić SL. Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite. in Polymers. 2022;14(1):18.
doi:10.3390/polym14010018 .

Filipović, Vuk V., Babić Radić, Marija M., Vuković, Jovana S., Vukomanović, Marija, Rubert, Marina, Hofmann, Sandra, Müller, Ralph, Tomić, Simonida Lj., "Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(beta-amino esters), and Hydroxyapatite" in Polymers, 14, no. 1 (2022):18,
https://doi.org/10.3390/polym14010018 . .

TY  - JOUR
AU  - Babić Radić, Marija M.
AU  - Filipovic, Vuk V.
AU  - Vukomanovic, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5023
AB  - The design and evaluation of novel 2-hydroxyethyl methacrylate/gelatin/alginate/graphene oxide hydrogels as innovative scaffolding biomaterials, which concurrently are the suitable drug delivery carrier, was proposed. The hydrogels were prepared by the adapted porogen leaching method; this is also the first time this method has been used to incorporate nanocolloidal graphene oxide through the hydrogel and simultaneously form porous structures. The effects of a material's composition on its chemical, morphological, mechanical, and swelling properties, as well as on cell viability and in vitro degradation, were assessed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), measurements of Young's modulus, gravimetric method and MTT test, respectively. The engineered hydrogels show good swelling capacity, fully hydrophilic surfaces, tunable porosity (from 56 to 76%) and mechanical properties (from 1.69 to 4.78 MPa), curcumin entrapment efficiency above 99% and excellent curcumin release performances. In vitro cytotoxicity on healthy human fibroblast (MRC5 cells) by MTT test reveal that the materials are nontoxic and biocompatible, proposing novel hydrogels for in vivo clinical evaluation to optimize tissue regeneration treatments by coupling the hydrogels with cells and different active agents to create material/biofactor hybrids with new levels of biofunctionality.
T2  - Gels
T1  - Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials
IS  - 1
SP  - 22
VL  - 8
DO  - 10.3390/gels8010022
ER  - 

@article{
author = "Babić Radić, Marija M. and Filipovic, Vuk V. and Vukomanovic, Marija and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2022",
abstract = "The design and evaluation of novel 2-hydroxyethyl methacrylate/gelatin/alginate/graphene oxide hydrogels as innovative scaffolding biomaterials, which concurrently are the suitable drug delivery carrier, was proposed. The hydrogels were prepared by the adapted porogen leaching method; this is also the first time this method has been used to incorporate nanocolloidal graphene oxide through the hydrogel and simultaneously form porous structures. The effects of a material's composition on its chemical, morphological, mechanical, and swelling properties, as well as on cell viability and in vitro degradation, were assessed using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), measurements of Young's modulus, gravimetric method and MTT test, respectively. The engineered hydrogels show good swelling capacity, fully hydrophilic surfaces, tunable porosity (from 56 to 76%) and mechanical properties (from 1.69 to 4.78 MPa), curcumin entrapment efficiency above 99% and excellent curcumin release performances. In vitro cytotoxicity on healthy human fibroblast (MRC5 cells) by MTT test reveal that the materials are nontoxic and biocompatible, proposing novel hydrogels for in vivo clinical evaluation to optimize tissue regeneration treatments by coupling the hydrogels with cells and different active agents to create material/biofactor hybrids with new levels of biofunctionality.",
journal = "Gels",
title = "Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials",
number = "1",
pages = "22",
volume = "8",
doi = "10.3390/gels8010022"
}

Babić Radić, M. M., Filipovic, V. V., Vukomanovic, M., Nikodinović-Runić, J.,& Tomić, S.. (2022). Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. in Gels, 8(1), 22.
https://doi.org/10.3390/gels8010022

Babić Radić MM, Filipovic VV, Vukomanovic M, Nikodinović-Runić J, Tomić S. Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials. in Gels. 2022;8(1):22.
doi:10.3390/gels8010022 .

Babić Radić, Marija M., Filipovic, Vuk V., Vukomanovic, Marija, Nikodinović-Runić, Jasmina, Tomić, Simonida, "Degradable 2-Hydroxyethyl Methacrylate/Gelatin/Alginate Hydrogels Infused by Nanocolloidal Graphene Oxide as Promising Drug Delivery and Scaffolding Biomaterials" in Gels, 8, no. 1 (2022):22,
https://doi.org/10.3390/gels8010022 . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Nikodinović-Runić, Jasmina
AU  - Vukomanovic, Marija
AU  - Babić, Marija M.
AU  - Vuković, Jovana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4926
AB  - Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.
T2  - Polymers
T1  - Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite
IS  - 6
SP  - 932
VL  - 13
DO  - 10.3390/polym13060932
ER  - 

@article{
author = "Tomić, Simonida and Nikodinović-Runić, Jasmina and Vukomanovic, Marija and Babić, Marija M. and Vuković, Jovana",
year = "2021",
abstract = "Hydrogel scaffolding biomaterials are one of the most attractive polymeric biomaterials for regenerative engineering and can be engineered into tissue mimetic scaffolds to support cell growth due to their similarity to the native extracellular matrix. The novel, versatile hydrogel scaffolds based on alginate, gelatin, 2-hydroxyethyl methacrylate, and inorganic agent hydroxyapatite were prepared by modified cryogelation. The chemical composition, morphology, porosity, mechanical properties, effects on cell viability, in vitro degradation, in vitro and in vivo biocompatibility were tested to correlate the material's composition with the corresponding properties. Scaffolds showed an interconnected porous microstructure, satisfactory mechanical strength, favorable hydrophilicity, degradation, and suitable in vitro and in vivo biocompatible behavior. Materials showed good biocompatibility with healthy human fibroblast in cell culture, as well as in vivo with zebrafish assay, suggesting newly synthesized hydrogel scaffolds as a potential new generation of hydrogel scaffolding biomaterials with tunable properties for versatile biomedical applications and tissue regeneration.",
journal = "Polymers",
title = "Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite",
number = "6",
pages = "932",
volume = "13",
doi = "10.3390/polym13060932"
}

Tomić, S., Nikodinović-Runić, J., Vukomanovic, M., Babić, M. M.,& Vuković, J.. (2021). Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. in Polymers, 13(6), 932.
https://doi.org/10.3390/polym13060932

Tomić S, Nikodinović-Runić J, Vukomanovic M, Babić MM, Vuković J. Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite. in Polymers. 2021;13(6):932.
doi:10.3390/polym13060932 .

Tomić, Simonida, Nikodinović-Runić, Jasmina, Vukomanovic, Marija, Babić, Marija M., Vuković, Jovana, "Novel Hydrogel Scaffolds Based on Alginate, Gelatin, 2-Hydroxyethyl Methacrylate, and Hydroxyapatite" in Polymers, 13, no. 6 (2021):932,
https://doi.org/10.3390/polym13060932 . .

TY  - JOUR
AU  - Jugović, Dragana
AU  - Mitrić, Miodrag
AU  - Milović, Miloš
AU  - Jokić, Bojan M.
AU  - Vukomanovic, Marija
AU  - Suvorov, Danilo
AU  - Uskoković, Dragan
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5712
AB  - In this study, nanocrystalline LiFePO4/C composite powder has been synthesized via a cellulose matrix-assisted method. In an experiment conducted under extreme conditions involving rapid heating, short high-temperature delay, and subsequent quenching, well-ordered 35-nm crystallites have been obtained within 5 min. A quantitative filter paper has served both as a template and carbon source. It degrades pyrolytically through fragmentation reactions and formation of volatiles when exposed to rapid heating, which further has an impact on powder morphology, as revealed by electron microscopy studies. The electrochemical measurements in terms of galvanostatic cycling have shown that the approach presented in this study may enable to reach good rate capability and excellent cycling stability. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Powder Technology
T1  - Properties of quenched LiFePO4/C powder obtained via cellulose matrix-assisted method
EP  - 544
SP  - 539
VL  - 246
DO  - 10.1016/j.powtec.2013.06.021
ER  - 

@article{
author = "Jugović, Dragana and Mitrić, Miodrag and Milović, Miloš and Jokić, Bojan M. and Vukomanovic, Marija and Suvorov, Danilo and Uskoković, Dragan",
year = "2013",
abstract = "In this study, nanocrystalline LiFePO4/C composite powder has been synthesized via a cellulose matrix-assisted method. In an experiment conducted under extreme conditions involving rapid heating, short high-temperature delay, and subsequent quenching, well-ordered 35-nm crystallites have been obtained within 5 min. A quantitative filter paper has served both as a template and carbon source. It degrades pyrolytically through fragmentation reactions and formation of volatiles when exposed to rapid heating, which further has an impact on powder morphology, as revealed by electron microscopy studies. The electrochemical measurements in terms of galvanostatic cycling have shown that the approach presented in this study may enable to reach good rate capability and excellent cycling stability. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Powder Technology",
title = "Properties of quenched LiFePO4/C powder obtained via cellulose matrix-assisted method",
pages = "544-539",
volume = "246",
doi = "10.1016/j.powtec.2013.06.021"
}

Jugović, D., Mitrić, M., Milović, M., Jokić, B. M., Vukomanovic, M., Suvorov, D.,& Uskoković, D.. (2013). Properties of quenched LiFePO4/C powder obtained via cellulose matrix-assisted method. in Powder Technology, 246, 539-544.
https://doi.org/10.1016/j.powtec.2013.06.021

Jugović D, Mitrić M, Milović M, Jokić BM, Vukomanovic M, Suvorov D, Uskoković D. Properties of quenched LiFePO4/C powder obtained via cellulose matrix-assisted method. in Powder Technology. 2013;246:539-544.
doi:10.1016/j.powtec.2013.06.021 .

Jugović, Dragana, Mitrić, Miodrag, Milović, Miloš, Jokić, Bojan M., Vukomanovic, Marija, Suvorov, Danilo, Uskoković, Dragan, "Properties of quenched LiFePO4/C powder obtained via cellulose matrix-assisted method" in Powder Technology, 246 (2013):539-544,
https://doi.org/10.1016/j.powtec.2013.06.021 . .