@article{
author = "Prlainović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica and Kozlowska, Roksana T. and Mijin, Dušan",
year = "2010",
abstract = "The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 degrees C with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.",
publisher = "Sociedade Brasileira de Química",
journal = "Journal of the Brazilian Chemical Society",
title = "A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone",
pages = "2293-2285",
number = "12",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1563"
}