TY  - CONF
AU  - Šekuljica, Nataša
AU  - Salim, Abdalla Ali
AU  - Luković, Nevena
AU  - Jovanović, Jelena
AU  - Jakovetić Tanasković, Sonja
AU  - Grbavčić, Sanja
AU  - Knežević-Jugović, Zorica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6274
AB  - Solid-state fermentation (SSF) is a fermentation process where different agro-industrial
residues have been used as substrates to maximize their utilizations and to address the waste
disposal issues. Bacillus sp. strain has been studied for its ability to produce different enzymes
by SSF of agro-industrial residues. Soybean meal (SBM) is a co-product after solvent
extraction process in dietary oil production and high quality source of protein.
Herein, SBM was assessed for the production of hydrolytic enzymes through SSF with
Bacillus sp. strain for 8 days at 30 °C. Highest obtained yields of producing proteases,
cellulases, α-amylases and pectinases were 56.4, 0.89, 0.95 and 1.07 U g-1, respectively. The
enzymes produced were very different in biochemical characteristics, including activities at
specific temperatures and a wide range of pH values. The highest activity proteases,
cellulases, α-amylases exhibited at higher temperatures, 50 °C suggesting the high potential of
these enzymes for industrial application involving elevated conditions. Fermented soybean
meal (FSBM) was examined in terms of total phenol content (TPC), scavenging DPPH and
ABTS radical abilities. Scavenging DPPH and ABTS abilities of FSBM were all stronger than
that of SBM. The radical scavenging ability increased by fermentation in accordance with the
increase in TPC content. TPC increase from 1.12 to 3.12 mgGAE/g soybean meal after three days
of fermentation was observed. The high hydrolytic enzyme activity and stability established
plus the low cost of the substrate make this solid-state process a promising tool for
biotechnological industry.
PB  - Bratislava : Slovak Society of Chemical Engineering
C3  - Proceedings / 46th International Conference of Slovak Society of Chemical Engineering (SSCHE 2019)
T1  - SOLID-STATE FERMENTATION OF SOYBEAN MEAL: HYDROLYTIC ENZYMES PRODUCTION AND IMPROVEMENT IN BIOACTIVITY
EP  - 110
SP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6274
ER  - 

@conference{
author = "Šekuljica, Nataša and Salim, Abdalla Ali and Luković, Nevena and Jovanović, Jelena and Jakovetić Tanasković, Sonja and Grbavčić, Sanja and Knežević-Jugović, Zorica",
year = "2019",
abstract = "Solid-state fermentation (SSF) is a fermentation process where different agro-industrial
residues have been used as substrates to maximize their utilizations and to address the waste
disposal issues. Bacillus sp. strain has been studied for its ability to produce different enzymes
by SSF of agro-industrial residues. Soybean meal (SBM) is a co-product after solvent
extraction process in dietary oil production and high quality source of protein.
Herein, SBM was assessed for the production of hydrolytic enzymes through SSF with
Bacillus sp. strain for 8 days at 30 °C. Highest obtained yields of producing proteases,
cellulases, α-amylases and pectinases were 56.4, 0.89, 0.95 and 1.07 U g-1, respectively. The
enzymes produced were very different in biochemical characteristics, including activities at
specific temperatures and a wide range of pH values. The highest activity proteases,
cellulases, α-amylases exhibited at higher temperatures, 50 °C suggesting the high potential of
these enzymes for industrial application involving elevated conditions. Fermented soybean
meal (FSBM) was examined in terms of total phenol content (TPC), scavenging DPPH and
ABTS radical abilities. Scavenging DPPH and ABTS abilities of FSBM were all stronger than
that of SBM. The radical scavenging ability increased by fermentation in accordance with the
increase in TPC content. TPC increase from 1.12 to 3.12 mgGAE/g soybean meal after three days
of fermentation was observed. The high hydrolytic enzyme activity and stability established
plus the low cost of the substrate make this solid-state process a promising tool for
biotechnological industry.",
publisher = "Bratislava : Slovak Society of Chemical Engineering",
journal = "Proceedings / 46th International Conference of Slovak Society of Chemical Engineering (SSCHE 2019)",
title = "SOLID-STATE FERMENTATION OF SOYBEAN MEAL: HYDROLYTIC ENZYMES PRODUCTION AND IMPROVEMENT IN BIOACTIVITY",
pages = "110-102",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6274"
}

Šekuljica, N., Salim, A. A., Luković, N., Jovanović, J., Jakovetić Tanasković, S., Grbavčić, S.,& Knežević-Jugović, Z.. (2019). SOLID-STATE FERMENTATION OF SOYBEAN MEAL: HYDROLYTIC ENZYMES PRODUCTION AND IMPROVEMENT IN BIOACTIVITY. in Proceedings / 46th International Conference of Slovak Society of Chemical Engineering (SSCHE 2019)
Bratislava : Slovak Society of Chemical Engineering., 102-110.
https://hdl.handle.net/21.15107/rcub_technorep_6274

Šekuljica N, Salim AA, Luković N, Jovanović J, Jakovetić Tanasković S, Grbavčić S, Knežević-Jugović Z. SOLID-STATE FERMENTATION OF SOYBEAN MEAL: HYDROLYTIC ENZYMES PRODUCTION AND IMPROVEMENT IN BIOACTIVITY. in Proceedings / 46th International Conference of Slovak Society of Chemical Engineering (SSCHE 2019). 2019;:102-110.
https://hdl.handle.net/21.15107/rcub_technorep_6274 .

Šekuljica, Nataša, Salim, Abdalla Ali, Luković, Nevena, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Grbavčić, Sanja, Knežević-Jugović, Zorica, "SOLID-STATE FERMENTATION OF SOYBEAN MEAL: HYDROLYTIC ENZYMES PRODUCTION AND IMPROVEMENT IN BIOACTIVITY" in Proceedings / 46th International Conference of Slovak Society of Chemical Engineering (SSCHE 2019) (2019):102-110,
https://hdl.handle.net/21.15107/rcub_technorep_6274 .

TY  - JOUR
AU  - Salim, Abdalla Ali
AU  - Grbavčić, Sanja
AU  - Šekuljica, Nataša
AU  - Vukašinović-Sekulić, Maja
AU  - Jovanović, Jelena
AU  - Jakovetić Tanasković, Sonja
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4065
AB  - Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU).
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods
EP  - 368
IS  - 3
SP  - 361
VL  - 66
DO  - 10.1002/bab.1732
ER  - 

@article{
author = "Salim, Abdalla Ali and Grbavčić, Sanja and Šekuljica, Nataša and Vukašinović-Sekulić, Maja and Jovanović, Jelena and Jakovetić Tanasković, Sonja and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2019",
abstract = "Protease, cellulase, and alpha-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and alpha-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity ( gt = 7.5 IU).",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods",
pages = "368-361",
number = "3",
volume = "66",
doi = "10.1002/bab.1732"
}

Salim, A. A., Grbavčić, S., Šekuljica, N., Vukašinović-Sekulić, M., Jovanović, J., Jakovetić Tanasković, S., Luković, N.,& Knežević-Jugović, Z.. (2019). Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 66(3), 361-368.
https://doi.org/10.1002/bab.1732

Salim AA, Grbavčić S, Šekuljica N, Vukašinović-Sekulić M, Jovanović J, Jakovetić Tanasković S, Luković N, Knežević-Jugović Z. Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods. in Biotechnology and Applied Biochemistry. 2019;66(3):361-368.
doi:10.1002/bab.1732 .

Salim, Abdalla Ali, Grbavčić, Sanja, Šekuljica, Nataša, Vukašinović-Sekulić, Maja, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Luković, Nevena, Knežević-Jugović, Zorica, "Enzyme production by solid-state fermentation on soybean meal: A comparative study of conventional and ultrasound-assisted extraction methods" in Biotechnology and Applied Biochemistry, 66, no. 3 (2019):361-368,
https://doi.org/10.1002/bab.1732 . .

TY  - JOUR
AU  - Salim, Abdalla Ali
AU  - Grbavčić, Sanja
AU  - Šekuljica, Nataša
AU  - Stefanović, Andrea
AU  - Jakovetić Tanasković, Sonja
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3687
AB  - Study on potential of different agro-industrial waste residues for supporting the growth of newly isolated Bacillus sp. TMF-1 strain under solid-state fermentation (SSF) was conducted aiming to produce several industrially valuable enzymes. Since the feasibility of the initial study was confirmed, further objectives included evaluation of several pretreatments of the studied agricultural by-products (soybean meal, sunflower meal, maize bran, maize pericarp, olive oil cake and wheat bran) on the microbial productivity as means of enhancing the yields of produced proteases, alpha-amylases, cellulases and pectinases. Among acid/alkaline treatment, ultrasound and microwave assisted methods, chemical treatments superiorly affected most of the studied substrates. Highest yields of produced proteases (50.5 IU g (1)) and alpha-amylases (50.75 IU g (1)) were achieved on alkaline treated corn pericarp. Alkaline treatment also promoted the secretion of cellulases on maize bran (1.19 IU g (1)). Highest yield of pectinases was obtained on untreated soybean meal (64.90 IU g (1)).
PB  - Elsevier Sci Ltd, Oxford
T2  - Bioresource Technology
T1  - Production of enzymes by a newly isolated Bacillus sp TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods
EP  - 200
SP  - 193
VL  - 228
DO  - 10.1016/j.biortech.2016.12.081
ER  - 

@article{
author = "Salim, Abdalla Ali and Grbavčić, Sanja and Šekuljica, Nataša and Stefanović, Andrea and Jakovetić Tanasković, Sonja and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2017",
abstract = "Study on potential of different agro-industrial waste residues for supporting the growth of newly isolated Bacillus sp. TMF-1 strain under solid-state fermentation (SSF) was conducted aiming to produce several industrially valuable enzymes. Since the feasibility of the initial study was confirmed, further objectives included evaluation of several pretreatments of the studied agricultural by-products (soybean meal, sunflower meal, maize bran, maize pericarp, olive oil cake and wheat bran) on the microbial productivity as means of enhancing the yields of produced proteases, alpha-amylases, cellulases and pectinases. Among acid/alkaline treatment, ultrasound and microwave assisted methods, chemical treatments superiorly affected most of the studied substrates. Highest yields of produced proteases (50.5 IU g (1)) and alpha-amylases (50.75 IU g (1)) were achieved on alkaline treated corn pericarp. Alkaline treatment also promoted the secretion of cellulases on maize bran (1.19 IU g (1)). Highest yield of pectinases was obtained on untreated soybean meal (64.90 IU g (1)).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Bioresource Technology",
title = "Production of enzymes by a newly isolated Bacillus sp TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods",
pages = "200-193",
volume = "228",
doi = "10.1016/j.biortech.2016.12.081"
}

Salim, A. A., Grbavčić, S., Šekuljica, N., Stefanović, A., Jakovetić Tanasković, S., Luković, N.,& Knežević-Jugović, Z.. (2017). Production of enzymes by a newly isolated Bacillus sp TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods. in Bioresource Technology
Elsevier Sci Ltd, Oxford., 228, 193-200.
https://doi.org/10.1016/j.biortech.2016.12.081

Salim AA, Grbavčić S, Šekuljica N, Stefanović A, Jakovetić Tanasković S, Luković N, Knežević-Jugović Z. Production of enzymes by a newly isolated Bacillus sp TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods. in Bioresource Technology. 2017;228:193-200.
doi:10.1016/j.biortech.2016.12.081 .

Salim, Abdalla Ali, Grbavčić, Sanja, Šekuljica, Nataša, Stefanović, Andrea, Jakovetić Tanasković, Sonja, Luković, Nevena, Knežević-Jugović, Zorica, "Production of enzymes by a newly isolated Bacillus sp TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods" in Bioresource Technology, 228 (2017):193-200,
https://doi.org/10.1016/j.biortech.2016.12.081 . .