TY  - JOUR
AU  - Popescu, A. C.
AU  - Sima, F.
AU  - Duta, L.
AU  - Popescu, A. C.
AU  - Mihailescu, Ion N.
AU  - Capitanu, D.
AU  - Mustata, R.
AU  - Sima, L. E.
AU  - Petrescu, S. M.
AU  - Janaćković, Đorđe
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1483
AB  - We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances.
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests
EP  - 5490
IS  - 10
SP  - 5486
VL  - 255
DO  - 10.1016/j.apsusc.2008.10.031
ER  - 

@article{
author = "Popescu, A. C. and Sima, F. and Duta, L. and Popescu, A. C. and Mihailescu, Ion N. and Capitanu, D. and Mustata, R. and Sima, L. E. and Petrescu, S. M. and Janaćković, Đorđe",
year = "2009",
abstract = "We report on the synthesis by pulsed laser deposition with a KrF* excimer laser source (lambda = 248 nm, tau = 25 ns) of bioglass thin films of 6P57 and 6P61 types. Physiology, viability, and proliferation of human osteoblast cells were determined by quantitative in vitro tests performed by flow cytometry on primary osteoblasts cultured on pulsed laser deposited bioglasses. Both types of glass films proved to be appropriate mediums for cell survival and proliferation. In a parallel investigation, cell morphology and adhesion to the surface was studied by fluorescence microscopy and scanning electron microscopy. Strong bonds between the materials and cells were found in both cases, as osteoblast pseudopodes penetrated deep into the material. According to our observations, the 6P57 glass films were superior with respect to viability and proliferation performances.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests",
pages = "5490-5486",
number = "10",
volume = "255",
doi = "10.1016/j.apsusc.2008.10.031"
}

Popescu, A. C., Sima, F., Duta, L., Popescu, A. C., Mihailescu, I. N., Capitanu, D., Mustata, R., Sima, L. E., Petrescu, S. M.,& Janaćković, Đ.. (2009). Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests. in Applied Surface Science
Elsevier, Amsterdam., 255(10), 5486-5490.
https://doi.org/10.1016/j.apsusc.2008.10.031

Popescu AC, Sima F, Duta L, Popescu AC, Mihailescu IN, Capitanu D, Mustata R, Sima LE, Petrescu SM, Janaćković Đ. Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests. in Applied Surface Science. 2009;255(10):5486-5490.
doi:10.1016/j.apsusc.2008.10.031 .

Popescu, A. C., Sima, F., Duta, L., Popescu, A. C., Mihailescu, Ion N., Capitanu, D., Mustata, R., Sima, L. E., Petrescu, S. M., Janaćković, Đorđe, "Biocompatible and bioactive nanostructured glass coatings synthesized by pulsed laser deposition: In vitro biological tests" in Applied Surface Science, 255, no. 10 (2009):5486-5490,
https://doi.org/10.1016/j.apsusc.2008.10.031 . .

TY  - JOUR
AU  - Floroian, L.
AU  - Savu, B.
AU  - Stanciu, G.
AU  - Popescu, A. C.
AU  - Sima, F.
AU  - Mihailescu, Ion N.
AU  - Mustata, R.
AU  - Sima, L. E.
AU  - Petrescu, M.
AU  - Tanasković, Dušanka
AU  - Janaćković, Đorđe
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1230
AB  - We report the synthesis by pulsed laser deposition of thin structures of two bioactive glasses belonging to the SiO2-Na2O-K2O-CaO-MgO-P2O5 system, on medical grade Ti substrates. We evaluated their biocompatibility after immersion in simulated body fluids and by performing cells adhesion tests. The Films were characterized by confocal scanning laser microscopy and Fourier transform infrared spectrometry, before and after 30 and 46 days immersion in fluids. Our studies demonstrated that deposited coatings were degraded in simulated fluids. A new apatite layer was synthesized by ions changing with the fluid during the decomposition of bioglasses. We investigated after immersion in fluids cells adhesion and the cytoskelet organization of synthesized structures, by fluorescence microscopy. A good adhesion to bioglass coatings was evidenced.
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests
EP  - 3062
IS  - 5
SP  - 3056
VL  - 255
DO  - 10.1016/j.apsusc.2008.08.105
ER  - 

@article{
author = "Floroian, L. and Savu, B. and Stanciu, G. and Popescu, A. C. and Sima, F. and Mihailescu, Ion N. and Mustata, R. and Sima, L. E. and Petrescu, M. and Tanasković, Dušanka and Janaćković, Đorđe",
year = "2008",
abstract = "We report the synthesis by pulsed laser deposition of thin structures of two bioactive glasses belonging to the SiO2-Na2O-K2O-CaO-MgO-P2O5 system, on medical grade Ti substrates. We evaluated their biocompatibility after immersion in simulated body fluids and by performing cells adhesion tests. The Films were characterized by confocal scanning laser microscopy and Fourier transform infrared spectrometry, before and after 30 and 46 days immersion in fluids. Our studies demonstrated that deposited coatings were degraded in simulated fluids. A new apatite layer was synthesized by ions changing with the fluid during the decomposition of bioglasses. We investigated after immersion in fluids cells adhesion and the cytoskelet organization of synthesized structures, by fluorescence microscopy. A good adhesion to bioglass coatings was evidenced.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests",
pages = "3062-3056",
number = "5",
volume = "255",
doi = "10.1016/j.apsusc.2008.08.105"
}

Floroian, L., Savu, B., Stanciu, G., Popescu, A. C., Sima, F., Mihailescu, I. N., Mustata, R., Sima, L. E., Petrescu, M., Tanasković, D.,& Janaćković, Đ.. (2008). Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests. in Applied Surface Science
Elsevier, Amsterdam., 255(5), 3056-3062.
https://doi.org/10.1016/j.apsusc.2008.08.105

Floroian L, Savu B, Stanciu G, Popescu AC, Sima F, Mihailescu IN, Mustata R, Sima LE, Petrescu M, Tanasković D, Janaćković Đ. Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests. in Applied Surface Science. 2008;255(5):3056-3062.
doi:10.1016/j.apsusc.2008.08.105 .

Floroian, L., Savu, B., Stanciu, G., Popescu, A. C., Sima, F., Mihailescu, Ion N., Mustata, R., Sima, L. E., Petrescu, M., Tanasković, Dušanka, Janaćković, Đorđe, "Nanostructured bioglass thin films synthesized by pulsed laser deposition: CSLM, FTIR investigations and in vitro biotests" in Applied Surface Science, 255, no. 5 (2008):3056-3062,
https://doi.org/10.1016/j.apsusc.2008.08.105 . .

TY  - JOUR
AU  - Tanasković, Dušanka
AU  - Jokić, Bojan
AU  - Socol, Gabriel
AU  - Popescu, A. C.
AU  - Mihailescu, Ion N.
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1149
AB  - Functionally graded glass-apatite multistructures were synthesized by pulsed laser deposition on Ti substrates. We used sintered targets of hydroxyapatite Ca-10(PO4)(6)(OH)(2), or bioglasses in the system SiO2-Na2O-K2O-CaO-MgO-P2O5 with SiO2 content of either 57 wt.% (6P57) or 61 wt.% (061). A UV KrF* (lambda = 248 nm, tau  gt  7 ns) excimer laser source was used for the multipulse laser ablation of the targets. The hydroxyapatite thin films were obtained in H2O Vapors, while the bioglass layers were deposited in O-2. Thin films of 6P61 were deposited in direct contact with Ti, because Ti and this glass have similar thermal expansion behaviors, which ensure good bioglass adhesion to the substrate. This glass, however, is not bioactive, so yet more depositions of 6P57 bioglass and/or hydroxyapatite thin films were performed. All structures with hydroxyapatite overcoating were post-treated in a flux of water vapors. The obtained multistructures were characterized by various techniques. Xray investigations of the coatings found small amounts of crystalline hydroxyapatite in the outer layers. The scanning electron microscopy analyses revealed homogeneous coatings with good adhesion to the Ti substrate. Our studies showed that the multistructures we had obtained were compatible with further use in biomimetic metallic implants with glass-apatite coating applications.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Surface Science
T1  - Synthesis of functionally graded bioactive glass-apatite multistructures on Ti substrates by pulsed laser deposition
EP  - 1282
IS  - 4
SP  - 1279
VL  - 254
DO  - 10.1016/j.apsusc.2007.08.009
ER  - 

@article{
author = "Tanasković, Dušanka and Jokić, Bojan and Socol, Gabriel and Popescu, A. C. and Mihailescu, Ion N. and Petrović, Rada and Janaćković, Đorđe",
year = "2007",
abstract = "Functionally graded glass-apatite multistructures were synthesized by pulsed laser deposition on Ti substrates. We used sintered targets of hydroxyapatite Ca-10(PO4)(6)(OH)(2), or bioglasses in the system SiO2-Na2O-K2O-CaO-MgO-P2O5 with SiO2 content of either 57 wt.% (6P57) or 61 wt.% (061). A UV KrF* (lambda = 248 nm, tau  gt  7 ns) excimer laser source was used for the multipulse laser ablation of the targets. The hydroxyapatite thin films were obtained in H2O Vapors, while the bioglass layers were deposited in O-2. Thin films of 6P61 were deposited in direct contact with Ti, because Ti and this glass have similar thermal expansion behaviors, which ensure good bioglass adhesion to the substrate. This glass, however, is not bioactive, so yet more depositions of 6P57 bioglass and/or hydroxyapatite thin films were performed. All structures with hydroxyapatite overcoating were post-treated in a flux of water vapors. The obtained multistructures were characterized by various techniques. Xray investigations of the coatings found small amounts of crystalline hydroxyapatite in the outer layers. The scanning electron microscopy analyses revealed homogeneous coatings with good adhesion to the Ti substrate. Our studies showed that the multistructures we had obtained were compatible with further use in biomimetic metallic implants with glass-apatite coating applications.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Surface Science",
title = "Synthesis of functionally graded bioactive glass-apatite multistructures on Ti substrates by pulsed laser deposition",
pages = "1282-1279",
number = "4",
volume = "254",
doi = "10.1016/j.apsusc.2007.08.009"
}

Tanasković, D., Jokić, B., Socol, G., Popescu, A. C., Mihailescu, I. N., Petrović, R.,& Janaćković, Đ.. (2007). Synthesis of functionally graded bioactive glass-apatite multistructures on Ti substrates by pulsed laser deposition. in Applied Surface Science
Elsevier Science Bv, Amsterdam., 254(4), 1279-1282.
https://doi.org/10.1016/j.apsusc.2007.08.009

Tanasković D, Jokić B, Socol G, Popescu AC, Mihailescu IN, Petrović R, Janaćković Đ. Synthesis of functionally graded bioactive glass-apatite multistructures on Ti substrates by pulsed laser deposition. in Applied Surface Science. 2007;254(4):1279-1282.
doi:10.1016/j.apsusc.2007.08.009 .

Tanasković, Dušanka, Jokić, Bojan, Socol, Gabriel, Popescu, A. C., Mihailescu, Ion N., Petrović, Rada, Janaćković, Đorđe, "Synthesis of functionally graded bioactive glass-apatite multistructures on Ti substrates by pulsed laser deposition" in Applied Surface Science, 254, no. 4 (2007):1279-1282,
https://doi.org/10.1016/j.apsusc.2007.08.009 . .