TY  - JOUR
AU  - Cvjetićanin, Milica
AU  - Ramić, Bojana
AU  - Milanović, Marija
AU  - Veljović, Đorđe
AU  - Anđelković, Aleksandar
AU  - Maletić, Snežana
AU  - Jevrosimov, Irina
AU  - Bajkin, Branislav
AU  - Gudurić, Vera
PY  - 2024-06
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7453
AB  - Objectives: To assess and compare the cell viability and ion release profiles of two conventional glass ionomer cements (GICs), Fuji IX and Ketac Molar EasyMix, modified with TiO2 and Mg-doped-HAp nanoparticles (NPs). Methods: TiO2 NPs, synthesized via a sol–gel method, and Mg-doped hydroxyapatite, synthesized via a hydrothermal process, were incorporated into GICs at a concentration of 5 wt.%. The biocompatibility of prepared materials was assessed by evaluating their effects on the viability of dental pulp stem cells (DPSCs), together with monitoring ion release profiles. Statistical analysis was performed using One-way analysis of variance, with significance level p < 0.05. Results: The addition of NPs did not significantly affect the biocompatibility of GICs, as evidenced by comparable decreased levels in cell viability to their original formulations. Distinct variations in cell viability were observed among Fuji IX and Ketac Molar, including their respective modifications. FUJI IX and its modification with TiO2 exhibited moderate decrease in cell viability, while other groups exhibited severe negative effects. While slight differences in ion release profiles were observed among the groups, significant variations compared to original cements were not achieved. Fluoride release exhibited an initial “burst release” within the initial 24 h in all samples, stabilizing over subsequent days. Conclusions: The addition of NPs did not compromise biocompatibility, nor anticariogenic potential of tested GICs. However, observed differences among FUJI IX and Ketac Molar, including their respective modifications, as well as induced low viability of DPSC by all tested groups, suggest the need for careful consideration of cement composition in their biological assessments. Clinical significance: The findings contribute to understanding the complex interaction between NPs and GIC matrices. However, the results should be interpreted recognizing the inherent limitations associated with in vitro studies. Further research avenues could explore long-term effects, in vivo performance, and potential clinical applications.
PB  - Elsevier Ltd.
T2  - Journal of Dentistry
T1  - Cell viability assessment and ion release profiles of GICs modified with TiO2- and Mg-doped hydroxyapatite nanoparticles
SP  - 105015
VL  - 145
DO  - 10.1016/j.jdent.2024.105015
ER  - 

@article{
author = "Cvjetićanin, Milica and Ramić, Bojana and Milanović, Marija and Veljović, Đorđe and Anđelković, Aleksandar and Maletić, Snežana and Jevrosimov, Irina and Bajkin, Branislav and Gudurić, Vera",
year = "2024-06",
abstract = "Objectives: To assess and compare the cell viability and ion release profiles of two conventional glass ionomer cements (GICs), Fuji IX and Ketac Molar EasyMix, modified with TiO2 and Mg-doped-HAp nanoparticles (NPs). Methods: TiO2 NPs, synthesized via a sol–gel method, and Mg-doped hydroxyapatite, synthesized via a hydrothermal process, were incorporated into GICs at a concentration of 5 wt.%. The biocompatibility of prepared materials was assessed by evaluating their effects on the viability of dental pulp stem cells (DPSCs), together with monitoring ion release profiles. Statistical analysis was performed using One-way analysis of variance, with significance level p < 0.05. Results: The addition of NPs did not significantly affect the biocompatibility of GICs, as evidenced by comparable decreased levels in cell viability to their original formulations. Distinct variations in cell viability were observed among Fuji IX and Ketac Molar, including their respective modifications. FUJI IX and its modification with TiO2 exhibited moderate decrease in cell viability, while other groups exhibited severe negative effects. While slight differences in ion release profiles were observed among the groups, significant variations compared to original cements were not achieved. Fluoride release exhibited an initial “burst release” within the initial 24 h in all samples, stabilizing over subsequent days. Conclusions: The addition of NPs did not compromise biocompatibility, nor anticariogenic potential of tested GICs. However, observed differences among FUJI IX and Ketac Molar, including their respective modifications, as well as induced low viability of DPSC by all tested groups, suggest the need for careful consideration of cement composition in their biological assessments. Clinical significance: The findings contribute to understanding the complex interaction between NPs and GIC matrices. However, the results should be interpreted recognizing the inherent limitations associated with in vitro studies. Further research avenues could explore long-term effects, in vivo performance, and potential clinical applications.",
publisher = "Elsevier Ltd.",
journal = "Journal of Dentistry",
title = "Cell viability assessment and ion release profiles of GICs modified with TiO2- and Mg-doped hydroxyapatite nanoparticles",
pages = "105015",
volume = "145",
doi = "10.1016/j.jdent.2024.105015"
}

Cvjetićanin, M., Ramić, B., Milanović, M., Veljović, Đ., Anđelković, A., Maletić, S., Jevrosimov, I., Bajkin, B.,& Gudurić, V.. (2024-06). Cell viability assessment and ion release profiles of GICs modified with TiO2- and Mg-doped hydroxyapatite nanoparticles. in Journal of Dentistry
Elsevier Ltd.., 145, 105015.
https://doi.org/10.1016/j.jdent.2024.105015

Cvjetićanin M, Ramić B, Milanović M, Veljović Đ, Anđelković A, Maletić S, Jevrosimov I, Bajkin B, Gudurić V. Cell viability assessment and ion release profiles of GICs modified with TiO2- and Mg-doped hydroxyapatite nanoparticles. in Journal of Dentistry. 2024;145:105015.
doi:10.1016/j.jdent.2024.105015 .

Cvjetićanin, Milica, Ramić, Bojana, Milanović, Marija, Veljović, Đorđe, Anđelković, Aleksandar, Maletić, Snežana, Jevrosimov, Irina, Bajkin, Branislav, Gudurić, Vera, "Cell viability assessment and ion release profiles of GICs modified with TiO2- and Mg-doped hydroxyapatite nanoparticles" in Journal of Dentistry, 145 (2024-06):105015,
https://doi.org/10.1016/j.jdent.2024.105015 . .