TY  - JOUR
AU  - Lu, Xiyang
AU  - Yao, Yao
AU  - Yang, Yang
AU  - Zhang, Zhongxi
AU  - Gu, Jinjie
AU  - Mojović, Ljiljana
AU  - Knežević-Jugović, Zorica
AU  - Baganz, Frank
AU  - Lye, Gary
AU  - Shi, Jiping
AU  - Hao, Jian
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/14
AB  - Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Ethylene glycol and glycolic acid production by wild-typeEscherichia coli
EP  - 755
IS  - 4
SP  - 744
SP  - 755
VL  - 68
DO  - 10.1002/bab.1987
ER  - 

@article{
author = "Lu, Xiyang and Yao, Yao and Yang, Yang and Zhang, Zhongxi and Gu, Jinjie and Mojović, Ljiljana and Knežević-Jugović, Zorica and Baganz, Frank and Lye, Gary and Shi, Jiping and Hao, Jian",
year = "2021",
abstract = "Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-typeEscherichia coliW3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore,E. colistrains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced byE. coli Delta yqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, theE. coli Delta aldA+yqhDproduced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production byE. colion xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli",
pages = "755-744-755",
number = "4",
volume = "68",
doi = "10.1002/bab.1987"
}

Lu, X., Yao, Y., Yang, Y., Zhang, Z., Gu, J., Mojović, L., Knežević-Jugović, Z., Baganz, F., Lye, G., Shi, J.,& Hao, J.. (2021). Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 68(4), 744-755.
https://doi.org/10.1002/bab.1987

Lu X, Yao Y, Yang Y, Zhang Z, Gu J, Mojović L, Knežević-Jugović Z, Baganz F, Lye G, Shi J, Hao J. Ethylene glycol and glycolic acid production by wild-typeEscherichia coli. in Biotechnology and Applied Biochemistry. 2021;68(4):744-755.
doi:10.1002/bab.1987 .

Lu, Xiyang, Yao, Yao, Yang, Yang, Zhang, Zhongxi, Gu, Jinjie, Mojović, Ljiljana, Knežević-Jugović, Zorica, Baganz, Frank, Lye, Gary, Shi, Jiping, Hao, Jian, "Ethylene glycol and glycolic acid production by wild-typeEscherichia coli" in Biotechnology and Applied Biochemistry, 68, no. 4 (2021):744-755,
https://doi.org/10.1002/bab.1987 . .

TY  - JOUR
AU  - Radetić, Tamara
AU  - Johnson, E.
AU  - Olmsted, D. L.
AU  - Yang, Yang
AU  - Laird, B. B.
AU  - Asta, M.
AU  - Dahmen, U.
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3618
AB  - We have made direct observations of the Brownian motion of individual nanosized liquid lead inclusions in solid aluminum by in-situ transmission electron microscopy. The process was found to depend strongly on the size of the inclusion and the anisotropy of the interfacial energy. The rate controlling mechanism was the nucleation of steps on facets of the equilibrium shape and the diffusion of Al along the liquid-solid interface. Because the Al-Pb interface undergoes a roughening transition at T-r approximate to 820 K, the step nucleation barrier decreases with increasing temperature and vanishes completely at Tr. By including the temperature dependence explicitly, we demonstrate that the contribution of the step energy to an Arrhenius plot of the data has a slope greater than the actual activation energy at temperature T by a factor T-r/(T-r-T). In addition, we show that the diffusion barrier for interfacial transport makes a substantial contribution. Our analysis reconciles a large discrepancy between activation energies obtained from the temperature and size dependences of the process and solves the apparent paradox posed by the observation that some particles are frozen in non-equilibrium shapes while nearby smaller particles are sufficiently mobile to undergo rapid Brownian motion.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Acta Materialia
T1  - Step-controlled Brownian motion of nanosized liquid Pb inclusions in a solid Al matrix
EP  - 433
SP  - 427
VL  - 141
DO  - 10.1016/j.actamat.2017.09.040
ER  - 

@article{
author = "Radetić, Tamara and Johnson, E. and Olmsted, D. L. and Yang, Yang and Laird, B. B. and Asta, M. and Dahmen, U.",
year = "2017",
abstract = "We have made direct observations of the Brownian motion of individual nanosized liquid lead inclusions in solid aluminum by in-situ transmission electron microscopy. The process was found to depend strongly on the size of the inclusion and the anisotropy of the interfacial energy. The rate controlling mechanism was the nucleation of steps on facets of the equilibrium shape and the diffusion of Al along the liquid-solid interface. Because the Al-Pb interface undergoes a roughening transition at T-r approximate to 820 K, the step nucleation barrier decreases with increasing temperature and vanishes completely at Tr. By including the temperature dependence explicitly, we demonstrate that the contribution of the step energy to an Arrhenius plot of the data has a slope greater than the actual activation energy at temperature T by a factor T-r/(T-r-T). In addition, we show that the diffusion barrier for interfacial transport makes a substantial contribution. Our analysis reconciles a large discrepancy between activation energies obtained from the temperature and size dependences of the process and solves the apparent paradox posed by the observation that some particles are frozen in non-equilibrium shapes while nearby smaller particles are sufficiently mobile to undergo rapid Brownian motion.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Acta Materialia",
title = "Step-controlled Brownian motion of nanosized liquid Pb inclusions in a solid Al matrix",
pages = "433-427",
volume = "141",
doi = "10.1016/j.actamat.2017.09.040"
}

Radetić, T., Johnson, E., Olmsted, D. L., Yang, Y., Laird, B. B., Asta, M.,& Dahmen, U.. (2017). Step-controlled Brownian motion of nanosized liquid Pb inclusions in a solid Al matrix. in Acta Materialia
Pergamon-Elsevier Science Ltd, Oxford., 141, 427-433.
https://doi.org/10.1016/j.actamat.2017.09.040

Radetić T, Johnson E, Olmsted DL, Yang Y, Laird BB, Asta M, Dahmen U. Step-controlled Brownian motion of nanosized liquid Pb inclusions in a solid Al matrix. in Acta Materialia. 2017;141:427-433.
doi:10.1016/j.actamat.2017.09.040 .

Radetić, Tamara, Johnson, E., Olmsted, D. L., Yang, Yang, Laird, B. B., Asta, M., Dahmen, U., "Step-controlled Brownian motion of nanosized liquid Pb inclusions in a solid Al matrix" in Acta Materialia, 141 (2017):427-433,
https://doi.org/10.1016/j.actamat.2017.09.040 . .