TY  - JOUR
AU  - Knezević, Aleksandar
AU  - Stajić, Mirjana
AU  - Jovanović, Vladimir M.
AU  - Kovacević, Visnja
AU  - Ćilerdžić, Jasmina
AU  - Milovanović, Ivan
AU  - Vukojević, Jelena
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3249
AB  - Wheat straw is the major crop residue in European countries which makes it the most promising material for bioconversion into biofuels. However, cellulose and hemicellulose are protected with lignin, so delignification is an inevitable phase in lignocellulose processing. The organisms predominantly responsible for its degradation are white-rot fungi and among them Trametes species represent promising degraders due to a well-developed ligninolytic enzyme system. Although numerous studies have confirmed that low molecular weight compounds can induce the production and activity of ligninolytic enzymes it is not clear how this reflects on the extent of delignification. The aim of the study was to assess the capacity of p-anisidine and veratryl alcohol to induce the production and activity of Mn-oxidizing peroxidases and laccases, and wheat straw delignification by six Trametes species. Significant inter- and intraspecific variations in activity and features of these enzymes were found, as well as differences in the potential of lignocellulose degradation in the presence or absence of inducers. Differences in the catalytic properties of synthesized enzyme isoforms strongly affected lignin degradation. Apart from enhanced lignin degradation, the addition of p-anisidine could significantly improve the selectivity of wheat straw ligninolysis, which was especially evident for T. hirsuta strains.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Induction of wheat straw delignification by Trametes species
VL  - 6
DO  - 10.1038/srep26529
ER  - 

@article{
author = "Knezević, Aleksandar and Stajić, Mirjana and Jovanović, Vladimir M. and Kovacević, Visnja and Ćilerdžić, Jasmina and Milovanović, Ivan and Vukojević, Jelena",
year = "2016",
abstract = "Wheat straw is the major crop residue in European countries which makes it the most promising material for bioconversion into biofuels. However, cellulose and hemicellulose are protected with lignin, so delignification is an inevitable phase in lignocellulose processing. The organisms predominantly responsible for its degradation are white-rot fungi and among them Trametes species represent promising degraders due to a well-developed ligninolytic enzyme system. Although numerous studies have confirmed that low molecular weight compounds can induce the production and activity of ligninolytic enzymes it is not clear how this reflects on the extent of delignification. The aim of the study was to assess the capacity of p-anisidine and veratryl alcohol to induce the production and activity of Mn-oxidizing peroxidases and laccases, and wheat straw delignification by six Trametes species. Significant inter- and intraspecific variations in activity and features of these enzymes were found, as well as differences in the potential of lignocellulose degradation in the presence or absence of inducers. Differences in the catalytic properties of synthesized enzyme isoforms strongly affected lignin degradation. Apart from enhanced lignin degradation, the addition of p-anisidine could significantly improve the selectivity of wheat straw ligninolysis, which was especially evident for T. hirsuta strains.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Induction of wheat straw delignification by Trametes species",
volume = "6",
doi = "10.1038/srep26529"
}

Knezević, A., Stajić, M., Jovanović, V. M., Kovacević, V., Ćilerdžić, J., Milovanović, I.,& Vukojević, J.. (2016). Induction of wheat straw delignification by Trametes species. in Scientific Reports
Nature Publishing Group, London., 6.
https://doi.org/10.1038/srep26529

Knezević A, Stajić M, Jovanović VM, Kovacević V, Ćilerdžić J, Milovanović I, Vukojević J. Induction of wheat straw delignification by Trametes species. in Scientific Reports. 2016;6.
doi:10.1038/srep26529 .

Knezević, Aleksandar, Stajić, Mirjana, Jovanović, Vladimir M., Kovacević, Visnja, Ćilerdžić, Jasmina, Milovanović, Ivan, Vukojević, Jelena, "Induction of wheat straw delignification by Trametes species" in Scientific Reports, 6 (2016),
https://doi.org/10.1038/srep26529 . .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Stajić, Mirjana
AU  - Ćilerdžić, Jasmina
AU  - Stanojković, Tatjana
AU  - Knezević, Aleksandar
AU  - Vukojević, Jelena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2689
AB  - The goal of this study was the evaluation of antifungal, antioxidant and anticancer potentials of Pleurotus eryngii, P. ostreatus and P. pulmonarius mycelial extracts, and the influence of mycelium enrichment with selenium on these activities. Both Se-amended and non-amended extracts showed the same or similar minimal inhibitory concentration for 14 studied micromycetes, while a fungicidal effect was not noted, contrary to ketoconazole, which had inhibitory and fungicidal effects at very low concentrations. Se-non-amended extracts exhibited antioxidant activity, especially at higher concentrations. Selenium enrichment influenced activity, its effects decreasing in P. eryngii and P. pulmonarius, while in P. ostreatus no effect was noted. The DPPH radical scavenging capacity of the extracts was in direct correlation with their phenol and flavonoid contents. Cytotoxic activity against both HeLa and LS174 cell lines was very low compared with cis-DDP. These features suggest that mycelium should be an object of intensive studies.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts
EP  - 1388
IS  - 4
SP  - 1379
VL  - 66
DO  - 10.2298/ABS1404379M
ER  - 

@article{
author = "Milovanović, Ivan and Stajić, Mirjana and Ćilerdžić, Jasmina and Stanojković, Tatjana and Knezević, Aleksandar and Vukojević, Jelena",
year = "2014",
abstract = "The goal of this study was the evaluation of antifungal, antioxidant and anticancer potentials of Pleurotus eryngii, P. ostreatus and P. pulmonarius mycelial extracts, and the influence of mycelium enrichment with selenium on these activities. Both Se-amended and non-amended extracts showed the same or similar minimal inhibitory concentration for 14 studied micromycetes, while a fungicidal effect was not noted, contrary to ketoconazole, which had inhibitory and fungicidal effects at very low concentrations. Se-non-amended extracts exhibited antioxidant activity, especially at higher concentrations. Selenium enrichment influenced activity, its effects decreasing in P. eryngii and P. pulmonarius, while in P. ostreatus no effect was noted. The DPPH radical scavenging capacity of the extracts was in direct correlation with their phenol and flavonoid contents. Cytotoxic activity against both HeLa and LS174 cell lines was very low compared with cis-DDP. These features suggest that mycelium should be an object of intensive studies.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts",
pages = "1388-1379",
number = "4",
volume = "66",
doi = "10.2298/ABS1404379M"
}

Milovanović, I., Stajić, M., Ćilerdžić, J., Stanojković, T., Knezević, A.,& Vukojević, J.. (2014). Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1379-1388.
https://doi.org/10.2298/ABS1404379M

Milovanović I, Stajić M, Ćilerdžić J, Stanojković T, Knezević A, Vukojević J. Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts. in Archives of Biological Sciences. 2014;66(4):1379-1388.
doi:10.2298/ABS1404379M .

Milovanović, Ivan, Stajić, Mirjana, Ćilerdžić, Jasmina, Stanojković, Tatjana, Knezević, Aleksandar, Vukojević, Jelena, "Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts" in Archives of Biological Sciences, 66, no. 4 (2014):1379-1388,
https://doi.org/10.2298/ABS1404379M . .

TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Milovanović, Ivan
AU  - Stajić, Mirjana
AU  - Lončar, Nikola L.
AU  - Brčeski, Ilija
AU  - Vukojević, Jelena
AU  - Ćilerdžić, Jasmina
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2404
AB  - As biological decomposition of plant biomass represents a popular alternative environmental-friendly and economically justified process, screening of ligninolytic enzyme systems of various fungal species is a topical study area. The goal of the study was to obtain clear insight into the dynamics of laccase, Mn-dependent peroxidase, and Mn-independent peroxidase activity and levels of wheat straw lignin degradation in seven wood-rotting fungi. The best laccase producers were Pleurotus ostreatus and Pleurotus eryngii. Lenzites betulinus and Fomitopsis pinicola were the best Mn-dependent peroxidase producers, and P. ostreatus the weakest one. The peak of Mn-independent peroxidase was noted in Dichomytus squalens, and the minimum value in P. ostreatus. The profiles of the three enzymes, obtained by isoelectric focusing, were variable depending on the species and cultivation period. D. squalens was the best lignin degrader (34.1% of total lignin amount), and P. ostreatus and P. eryngii the weakest ones (7.1% and 14.5%, respectively).
PB  - Elsevier Sci Ltd, Oxford
T2  - Bioresource Technology
T1  - Lignin degradation by selected fungal species
EP  - 123
SP  - 117
VL  - 138
DO  - 10.1016/j.biortech.2013.03.182
ER  - 

@article{
author = "Knežević, Aleksandar and Milovanović, Ivan and Stajić, Mirjana and Lončar, Nikola L. and Brčeski, Ilija and Vukojević, Jelena and Ćilerdžić, Jasmina",
year = "2013",
abstract = "As biological decomposition of plant biomass represents a popular alternative environmental-friendly and economically justified process, screening of ligninolytic enzyme systems of various fungal species is a topical study area. The goal of the study was to obtain clear insight into the dynamics of laccase, Mn-dependent peroxidase, and Mn-independent peroxidase activity and levels of wheat straw lignin degradation in seven wood-rotting fungi. The best laccase producers were Pleurotus ostreatus and Pleurotus eryngii. Lenzites betulinus and Fomitopsis pinicola were the best Mn-dependent peroxidase producers, and P. ostreatus the weakest one. The peak of Mn-independent peroxidase was noted in Dichomytus squalens, and the minimum value in P. ostreatus. The profiles of the three enzymes, obtained by isoelectric focusing, were variable depending on the species and cultivation period. D. squalens was the best lignin degrader (34.1% of total lignin amount), and P. ostreatus and P. eryngii the weakest ones (7.1% and 14.5%, respectively).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Bioresource Technology",
title = "Lignin degradation by selected fungal species",
pages = "123-117",
volume = "138",
doi = "10.1016/j.biortech.2013.03.182"
}

Knežević, A., Milovanović, I., Stajić, M., Lončar, N. L., Brčeski, I., Vukojević, J.,& Ćilerdžić, J.. (2013). Lignin degradation by selected fungal species. in Bioresource Technology
Elsevier Sci Ltd, Oxford., 138, 117-123.
https://doi.org/10.1016/j.biortech.2013.03.182

Knežević A, Milovanović I, Stajić M, Lončar NL, Brčeski I, Vukojević J, Ćilerdžić J. Lignin degradation by selected fungal species. in Bioresource Technology. 2013;138:117-123.
doi:10.1016/j.biortech.2013.03.182 .

Knežević, Aleksandar, Milovanović, Ivan, Stajić, Mirjana, Lončar, Nikola L., Brčeski, Ilija, Vukojević, Jelena, Ćilerdžić, Jasmina, "Lignin degradation by selected fungal species" in Bioresource Technology, 138 (2013):117-123,
https://doi.org/10.1016/j.biortech.2013.03.182 . .