TY  - JOUR
AU  - Pejin, Jelena
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Kocić-Tanackov, Sunčica
AU  - Savić, Dragiša
AU  - Nikolić, Svetlana
AU  - Đukić-Vuković, Aleksandra
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3022
AB  - The aim of this study was to determine the effect of magnesium or calcium ions content in triticale mashes on glucose and maltose content after liquefaction as well as on bioethanol yield after fermentation. Triticale variety Odyssey was used in this study. Liquefaction and saccharification in this study were performed without using any additional saccharifying enzymes, i.e. the triticale starch was hydrolyzed only by the enzymes present in triticale grain. Glucose and maltose content increased with the increase of magnesium and calcium ion content in mash. Glucose and maltose content increased by 30.16% and 9.58%, respectively, when 160 mg/L of magnesium ions were added, compared to the control sample. Glucose and maltose content increased by 69.31% and 61.66%, respectively, when 160 mg/L of calcium ions were added, compared to the control sample. According to the obtained results for glucose and maltose content increase during liquefaction, the supplementation of mashes with calcium ions had greater influence on the activity of triticale's amylases than the supplementation of mashes with magnesium ions. The present investigation shows that magnesium and calcium ions addition to triticale mashes improved bioethanol production during SSF processing. When 160 mg/L of magnesium ions were added bioethanol content increased by 31.22% compared to the control sample while when 160 mg/L of calcium ions were added bioethanol content increased by 21.04%. High percentage of the theoretical bioethanol yield (92.19%) was achieved after fermentation when 160 mg/L of magnesium ions were added to triticale mash. The obtained results show that the addition of magnesium and calcium ions in bioethanol production from triticale increase triticale's amylase activity as well as yeast enzyme activity. All this shows that when triticale with high amylolytic enzymes activity is used in bioethanol production with the addition of magnesium ions there is no need to use commercial enzymes in starch hydrolysis, which makes the use of triticale as a raw material for bioethanol production more economical.
PB  - Elsevier Sci Ltd, Oxford
T2  - Fuel
T1  - Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition
EP  - 64
SP  - 58
VL  - 142
DO  - 10.1016/j.fuel.2014.10.077
ER  - 

@article{
author = "Pejin, Jelena and Mojović, Ljiljana and Pejin, Dušanka and Kocić-Tanackov, Sunčica and Savić, Dragiša and Nikolić, Svetlana and Đukić-Vuković, Aleksandra",
year = "2015",
abstract = "The aim of this study was to determine the effect of magnesium or calcium ions content in triticale mashes on glucose and maltose content after liquefaction as well as on bioethanol yield after fermentation. Triticale variety Odyssey was used in this study. Liquefaction and saccharification in this study were performed without using any additional saccharifying enzymes, i.e. the triticale starch was hydrolyzed only by the enzymes present in triticale grain. Glucose and maltose content increased with the increase of magnesium and calcium ion content in mash. Glucose and maltose content increased by 30.16% and 9.58%, respectively, when 160 mg/L of magnesium ions were added, compared to the control sample. Glucose and maltose content increased by 69.31% and 61.66%, respectively, when 160 mg/L of calcium ions were added, compared to the control sample. According to the obtained results for glucose and maltose content increase during liquefaction, the supplementation of mashes with calcium ions had greater influence on the activity of triticale's amylases than the supplementation of mashes with magnesium ions. The present investigation shows that magnesium and calcium ions addition to triticale mashes improved bioethanol production during SSF processing. When 160 mg/L of magnesium ions were added bioethanol content increased by 31.22% compared to the control sample while when 160 mg/L of calcium ions were added bioethanol content increased by 21.04%. High percentage of the theoretical bioethanol yield (92.19%) was achieved after fermentation when 160 mg/L of magnesium ions were added to triticale mash. The obtained results show that the addition of magnesium and calcium ions in bioethanol production from triticale increase triticale's amylase activity as well as yeast enzyme activity. All this shows that when triticale with high amylolytic enzymes activity is used in bioethanol production with the addition of magnesium ions there is no need to use commercial enzymes in starch hydrolysis, which makes the use of triticale as a raw material for bioethanol production more economical.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fuel",
title = "Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition",
pages = "64-58",
volume = "142",
doi = "10.1016/j.fuel.2014.10.077"
}

Pejin, J., Mojović, L., Pejin, D., Kocić-Tanackov, S., Savić, D., Nikolić, S.,& Đukić-Vuković, A.. (2015). Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition. in Fuel
Elsevier Sci Ltd, Oxford., 142, 58-64.
https://doi.org/10.1016/j.fuel.2014.10.077

Pejin J, Mojović L, Pejin D, Kocić-Tanackov S, Savić D, Nikolić S, Đukić-Vuković A. Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition. in Fuel. 2015;142:58-64.
doi:10.1016/j.fuel.2014.10.077 .

Pejin, Jelena, Mojović, Ljiljana, Pejin, Dušanka, Kocić-Tanackov, Sunčica, Savić, Dragiša, Nikolić, Svetlana, Đukić-Vuković, Aleksandra, "Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition" in Fuel, 142 (2015):58-64,
https://doi.org/10.1016/j.fuel.2014.10.077 . .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Grujić, Olgica
AU  - Markov, Siniša
AU  - Pejin, Jelena
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Nikolić, Svetlana
AU  - Savić, Dragiša
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1446
AB  - Bioethanol produced from renewable biomass, such as sugar, starch, or lignocellulosic materials, is one of the alternative energy resources, which is both renewable and environmentally friendly. Although, the priority in global future ethanol production is put on lignocellulosic processing, which is considered as one of the most promising second-generation biofuel technologies, the utilization of lignocellulosic material for fuel ethanol is still under improvement. Sugar-based (molasses, sugar cane, sugar beet) and starch-based (corn, wheat, triticale, potato, rice, etc.) feedstock are still currently predominant at the industrial level and they are, so far, economically favorable compared to lingocelluloses. Currently, approx. 80 % of total world ethanol production is obtained from the fermentation of simple sugars by yeast. In Serbia, one of the most suitable and available agricultural raw material for the industrial ethanol production are cereals such as corn, wheat and triticale. In addition, surpluses of this feedstock are being produced in our country constantly. In this paper, a brief review of the state of the art in bioethanol production and biomass availability is given, pointing out the progress possibilities on starch-based production. The progress possibilities are discussed in the domain of feedstock choice and pretreatment, optimization of fermentation, process integration and utilization of the process byproducts.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Progress in the production of bioethanol on starch-based feedstocks
EP  - 226
IS  - 4
SP  - 211
VL  - 15
DO  - 10.2298/CICEQ0904211M
ER  - 

@article{
author = "Mojović, Ljiljana and Pejin, Dušanka and Grujić, Olgica and Markov, Siniša and Pejin, Jelena and Rakin, Marica and Vukašinović-Sekulić, Maja and Nikolić, Svetlana and Savić, Dragiša",
year = "2009",
abstract = "Bioethanol produced from renewable biomass, such as sugar, starch, or lignocellulosic materials, is one of the alternative energy resources, which is both renewable and environmentally friendly. Although, the priority in global future ethanol production is put on lignocellulosic processing, which is considered as one of the most promising second-generation biofuel technologies, the utilization of lignocellulosic material for fuel ethanol is still under improvement. Sugar-based (molasses, sugar cane, sugar beet) and starch-based (corn, wheat, triticale, potato, rice, etc.) feedstock are still currently predominant at the industrial level and they are, so far, economically favorable compared to lingocelluloses. Currently, approx. 80 % of total world ethanol production is obtained from the fermentation of simple sugars by yeast. In Serbia, one of the most suitable and available agricultural raw material for the industrial ethanol production are cereals such as corn, wheat and triticale. In addition, surpluses of this feedstock are being produced in our country constantly. In this paper, a brief review of the state of the art in bioethanol production and biomass availability is given, pointing out the progress possibilities on starch-based production. The progress possibilities are discussed in the domain of feedstock choice and pretreatment, optimization of fermentation, process integration and utilization of the process byproducts.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Progress in the production of bioethanol on starch-based feedstocks",
pages = "226-211",
number = "4",
volume = "15",
doi = "10.2298/CICEQ0904211M"
}

Mojović, L., Pejin, D., Grujić, O., Markov, S., Pejin, J., Rakin, M., Vukašinović-Sekulić, M., Nikolić, S.,& Savić, D.. (2009). Progress in the production of bioethanol on starch-based feedstocks. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 15(4), 211-226.
https://doi.org/10.2298/CICEQ0904211M

Mojović L, Pejin D, Grujić O, Markov S, Pejin J, Rakin M, Vukašinović-Sekulić M, Nikolić S, Savić D. Progress in the production of bioethanol on starch-based feedstocks. in Chemical Industry & Chemical Engineering Quarterly. 2009;15(4):211-226.
doi:10.2298/CICEQ0904211M .

Mojović, Ljiljana, Pejin, Dušanka, Grujić, Olgica, Markov, Siniša, Pejin, Jelena, Rakin, Marica, Vukašinović-Sekulić, Maja, Nikolić, Svetlana, Savić, Dragiša, "Progress in the production of bioethanol on starch-based feedstocks" in Chemical Industry & Chemical Engineering Quarterly, 15, no. 4 (2009):211-226,
https://doi.org/10.2298/CICEQ0904211M . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Savić, Dragiša
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1340
AB  - A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal
EP  - 234
IS  - 4
SP  - 231
VL  - 14
DO  - 10.2298/CICEQ0804231N
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Savić, Dragiša",
year = "2008",
abstract = "A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal",
pages = "234-231",
number = "4",
volume = "14",
doi = "10.2298/CICEQ0804231N"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Savić, D.. (2008). A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 14(4), 231-234.
https://doi.org/10.2298/CICEQ0804231N

Nikolić S, Mojović L, Rakin M, Pejin D, Savić D. A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal. in Chemical Industry & Chemical Engineering Quarterly. 2008;14(4):231-234.
doi:10.2298/CICEQ0804231N .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Savić, Dragiša, "A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal" in Chemical Industry & Chemical Engineering Quarterly, 14, no. 4 (2008):231-234,
https://doi.org/10.2298/CICEQ0804231N . .

TY  - JOUR
AU  - Joković, Nataša
AU  - Savić, Dragiša
AU  - Vukašinović-Sekulić, Maja
AU  - Topisirović, Ljubiša
PY  - 2005
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/800
AB  - Isolation of lactic acid bacteria from artisanal dairy products is base for selection of new strains with properties which are important for their industrial application. In this work, characterization of 69 strains isolated from home-made cheese from mountain Radan was done. Characterization of isolates included examination of their acidifying activity, proteolytic activity and production of diacetyl exopolysaccharides and antimicrobial substances. Isolates from genus Lactococcus showed the best acidifying activity, while lactobacilli grew slowly in milk. The most isolates from genera Lactococcus i Lactobacillus synthesized extracellular proteinases, while isolates identified as Enterococcus faecium and Leuconostoc mesenteroides showed weak acidifying activity. Two isolates of Lactococcus lactis subsp. lactis biovar. diacetylactis, all isolates of En. faecium with typical phenotypical characteristics and 3 isolates of the genus Lactobacillus were able to produce diacetyl. All isolates identified as Ln. mesenteroides (except one identified as subspecies cremoris) were able to produce dextran growing on medium with sucrose and also some isolates from the genus Lactobacillus produced exopolysaccharides. Only two isolates of En. faecium produced bacteriocins.
AB  - U ovom radu urađena je karakterizacija 69 izolata bakterija mlečne kiseline izolovanih iz domaćeg sira sa planine Radan. Karakterizacija izolata je obuhvatala ispitivanje aktivnosti izolata u mleku kao i sintezu diacetila egzopolisaharida i bakteriocina. Izolati iz roda Lactococcus najviše snižavaju pH vrednost inokulisanog mleka, dok laktobacili najslabije rastu u mleku. Sinteza diacetila je detektovana kod dva izolata koja pripadaju biovarijetetu Lactococcus lactis subsp. lactis biovar. diacetylactis, kod svih izolata iz roda Enterococcus sa tipičnim fenotipskim karakteristikama i kod tri izolata iz roda Lactobacillus. Svi izolati koji pripadaju vrsti Ln. mesenteroides (osim jednog koji pripada podvrsti cremoris) poseduju sposobnost sinteze dekstrana prilikom rasta na podlozi sa saharozom, dok nekoliko izolata iz roda Lactobacillus pokazuju sposobnost sinteze egzopolisaharida. Bakteriocine sintetišu samo dva izolata koja pripadaju vrsti En. faecium.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Technological characterization of lactic acid bacteria isolated from Radan’s cheese
T1  - Karakterizacija tehnoloških osobina bakterija mlečne kiseline izolovanih iz sira sa planine Radan
EP  - 126
IS  - 14
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_technorep_800
ER  - 

@article{
author = "Joković, Nataša and Savić, Dragiša and Vukašinović-Sekulić, Maja and Topisirović, Ljubiša",
year = "2005",
abstract = "Isolation of lactic acid bacteria from artisanal dairy products is base for selection of new strains with properties which are important for their industrial application. In this work, characterization of 69 strains isolated from home-made cheese from mountain Radan was done. Characterization of isolates included examination of their acidifying activity, proteolytic activity and production of diacetyl exopolysaccharides and antimicrobial substances. Isolates from genus Lactococcus showed the best acidifying activity, while lactobacilli grew slowly in milk. The most isolates from genera Lactococcus i Lactobacillus synthesized extracellular proteinases, while isolates identified as Enterococcus faecium and Leuconostoc mesenteroides showed weak acidifying activity. Two isolates of Lactococcus lactis subsp. lactis biovar. diacetylactis, all isolates of En. faecium with typical phenotypical characteristics and 3 isolates of the genus Lactobacillus were able to produce diacetyl. All isolates identified as Ln. mesenteroides (except one identified as subspecies cremoris) were able to produce dextran growing on medium with sucrose and also some isolates from the genus Lactobacillus produced exopolysaccharides. Only two isolates of En. faecium produced bacteriocins., U ovom radu urađena je karakterizacija 69 izolata bakterija mlečne kiseline izolovanih iz domaćeg sira sa planine Radan. Karakterizacija izolata je obuhvatala ispitivanje aktivnosti izolata u mleku kao i sintezu diacetila egzopolisaharida i bakteriocina. Izolati iz roda Lactococcus najviše snižavaju pH vrednost inokulisanog mleka, dok laktobacili najslabije rastu u mleku. Sinteza diacetila je detektovana kod dva izolata koja pripadaju biovarijetetu Lactococcus lactis subsp. lactis biovar. diacetylactis, kod svih izolata iz roda Enterococcus sa tipičnim fenotipskim karakteristikama i kod tri izolata iz roda Lactobacillus. Svi izolati koji pripadaju vrsti Ln. mesenteroides (osim jednog koji pripada podvrsti cremoris) poseduju sposobnost sinteze dekstrana prilikom rasta na podlozi sa saharozom, dok nekoliko izolata iz roda Lactobacillus pokazuju sposobnost sinteze egzopolisaharida. Bakteriocine sintetišu samo dva izolata koja pripadaju vrsti En. faecium.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Technological characterization of lactic acid bacteria isolated from Radan’s cheese, Karakterizacija tehnoloških osobina bakterija mlečne kiseline izolovanih iz sira sa planine Radan",
pages = "126-115",
number = "14",
url = "https://hdl.handle.net/21.15107/rcub_technorep_800"
}

Joković, N., Savić, D., Vukašinović-Sekulić, M.,& Topisirović, L.. (2005). Technological characterization of lactic acid bacteria isolated from Radan’s cheese. in Zbornik radova Tehnološkog fakulteta, Leskovac
Univerzitet u Nišu - Tehnološki fakultet, Leskovac.(14), 115-126.
https://hdl.handle.net/21.15107/rcub_technorep_800

Joković N, Savić D, Vukašinović-Sekulić M, Topisirović L. Technological characterization of lactic acid bacteria isolated from Radan’s cheese. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2005;(14):115-126.
https://hdl.handle.net/21.15107/rcub_technorep_800 .

Joković, Nataša, Savić, Dragiša, Vukašinović-Sekulić, Maja, Topisirović, Ljubiša, "Technological characterization of lactic acid bacteria isolated from Radan’s cheese" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 14 (2005):115-126,
https://hdl.handle.net/21.15107/rcub_technorep_800 .