TY  - JOUR
AU  - Pejin, Jelena
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Kocić-Tanackov, Sunčica
AU  - Savić, Dragiša
AU  - Nikolić, Svetlana
AU  - Đukić-Vuković, Aleksandra
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3022
AB  - The aim of this study was to determine the effect of magnesium or calcium ions content in triticale mashes on glucose and maltose content after liquefaction as well as on bioethanol yield after fermentation. Triticale variety Odyssey was used in this study. Liquefaction and saccharification in this study were performed without using any additional saccharifying enzymes, i.e. the triticale starch was hydrolyzed only by the enzymes present in triticale grain. Glucose and maltose content increased with the increase of magnesium and calcium ion content in mash. Glucose and maltose content increased by 30.16% and 9.58%, respectively, when 160 mg/L of magnesium ions were added, compared to the control sample. Glucose and maltose content increased by 69.31% and 61.66%, respectively, when 160 mg/L of calcium ions were added, compared to the control sample. According to the obtained results for glucose and maltose content increase during liquefaction, the supplementation of mashes with calcium ions had greater influence on the activity of triticale's amylases than the supplementation of mashes with magnesium ions. The present investigation shows that magnesium and calcium ions addition to triticale mashes improved bioethanol production during SSF processing. When 160 mg/L of magnesium ions were added bioethanol content increased by 31.22% compared to the control sample while when 160 mg/L of calcium ions were added bioethanol content increased by 21.04%. High percentage of the theoretical bioethanol yield (92.19%) was achieved after fermentation when 160 mg/L of magnesium ions were added to triticale mash. The obtained results show that the addition of magnesium and calcium ions in bioethanol production from triticale increase triticale's amylase activity as well as yeast enzyme activity. All this shows that when triticale with high amylolytic enzymes activity is used in bioethanol production with the addition of magnesium ions there is no need to use commercial enzymes in starch hydrolysis, which makes the use of triticale as a raw material for bioethanol production more economical.
PB  - Elsevier Sci Ltd, Oxford
T2  - Fuel
T1  - Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition
EP  - 64
SP  - 58
VL  - 142
DO  - 10.1016/j.fuel.2014.10.077
ER  - 

@article{
author = "Pejin, Jelena and Mojović, Ljiljana and Pejin, Dušanka and Kocić-Tanackov, Sunčica and Savić, Dragiša and Nikolić, Svetlana and Đukić-Vuković, Aleksandra",
year = "2015",
abstract = "The aim of this study was to determine the effect of magnesium or calcium ions content in triticale mashes on glucose and maltose content after liquefaction as well as on bioethanol yield after fermentation. Triticale variety Odyssey was used in this study. Liquefaction and saccharification in this study were performed without using any additional saccharifying enzymes, i.e. the triticale starch was hydrolyzed only by the enzymes present in triticale grain. Glucose and maltose content increased with the increase of magnesium and calcium ion content in mash. Glucose and maltose content increased by 30.16% and 9.58%, respectively, when 160 mg/L of magnesium ions were added, compared to the control sample. Glucose and maltose content increased by 69.31% and 61.66%, respectively, when 160 mg/L of calcium ions were added, compared to the control sample. According to the obtained results for glucose and maltose content increase during liquefaction, the supplementation of mashes with calcium ions had greater influence on the activity of triticale's amylases than the supplementation of mashes with magnesium ions. The present investigation shows that magnesium and calcium ions addition to triticale mashes improved bioethanol production during SSF processing. When 160 mg/L of magnesium ions were added bioethanol content increased by 31.22% compared to the control sample while when 160 mg/L of calcium ions were added bioethanol content increased by 21.04%. High percentage of the theoretical bioethanol yield (92.19%) was achieved after fermentation when 160 mg/L of magnesium ions were added to triticale mash. The obtained results show that the addition of magnesium and calcium ions in bioethanol production from triticale increase triticale's amylase activity as well as yeast enzyme activity. All this shows that when triticale with high amylolytic enzymes activity is used in bioethanol production with the addition of magnesium ions there is no need to use commercial enzymes in starch hydrolysis, which makes the use of triticale as a raw material for bioethanol production more economical.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fuel",
title = "Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition",
pages = "64-58",
volume = "142",
doi = "10.1016/j.fuel.2014.10.077"
}

Pejin, J., Mojović, L., Pejin, D., Kocić-Tanackov, S., Savić, D., Nikolić, S.,& Đukić-Vuković, A.. (2015). Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition. in Fuel
Elsevier Sci Ltd, Oxford., 142, 58-64.
https://doi.org/10.1016/j.fuel.2014.10.077

Pejin J, Mojović L, Pejin D, Kocić-Tanackov S, Savić D, Nikolić S, Đukić-Vuković A. Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition. in Fuel. 2015;142:58-64.
doi:10.1016/j.fuel.2014.10.077 .

Pejin, Jelena, Mojović, Ljiljana, Pejin, Dušanka, Kocić-Tanackov, Sunčica, Savić, Dragiša, Nikolić, Svetlana, Đukić-Vuković, Aleksandra, "Bioethanol production from triticale by simultaneous saccharification and fermentation with magnesium or calcium ions addition" in Fuel, 142 (2015):58-64,
https://doi.org/10.1016/j.fuel.2014.10.077 . .

TY  - CONF
AU  - Marković, Milica
AU  - Markov, Siniša
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Pejin, Jelena
AU  - Vukašinović-Sekulić, Maja
AU  - Đukić-Vuković, Aleksandra
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6201
AB  - Triticale stillage is a by-product of the bioethanol fermentation. It is used as the
addition to feed and needs the protection from spoiling and pathogens. Lactic acid
bacteria can be used for it, and triticale stillage is then used as a medium for their
growth and the lactic acid production. The aim of this work was to see if there is a
possibility for scale up of the lactic acid fermentation process (by increasing 10 times
medium volume), when an inoculum is Lactobacillus fermentum PL-1. Titrable acidity
and pH were measured during the lactic acid fermentation. Utilization of carbohydrates
and the concentrations of D- and L- lactic acid were determined by enzymatic kits.
Viability of the lactic acid bacteria cell was done by pour a plate method. There was no
difference in pH value during the process (medium volume changed from 25 to 250
mL), while titrable acidity was significantly lower. The L-lactic acid yield was also
smaller, but not significantly. Utilization of carbohydrates was complete in both cases,
and viability was better in the samples with the usual experimental design. Given
results indicate that the volume of the fermentation medium can be increased on a pilot
level, for example.
PB  - Технолошки факултет, Лесковац
C3  - X SIMPOZIJUM «SAVREMENE TEHNOLOGIJE I PRIVREDNI RAZVOJ»
T1  - Lactic acid fermentation by Lactobacillus fermentum PL1 using a different volume of triticale stillage
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6201
ER  - 

@conference{
author = "Marković, Milica and Markov, Siniša and Pejin, Dušanka and Mojović, Ljiljana and Pejin, Jelena and Vukašinović-Sekulić, Maja and Đukić-Vuković, Aleksandra",
year = "2013",
abstract = "Triticale stillage is a by-product of the bioethanol fermentation. It is used as the
addition to feed and needs the protection from spoiling and pathogens. Lactic acid
bacteria can be used for it, and triticale stillage is then used as a medium for their
growth and the lactic acid production. The aim of this work was to see if there is a
possibility for scale up of the lactic acid fermentation process (by increasing 10 times
medium volume), when an inoculum is Lactobacillus fermentum PL-1. Titrable acidity
and pH were measured during the lactic acid fermentation. Utilization of carbohydrates
and the concentrations of D- and L- lactic acid were determined by enzymatic kits.
Viability of the lactic acid bacteria cell was done by pour a plate method. There was no
difference in pH value during the process (medium volume changed from 25 to 250
mL), while titrable acidity was significantly lower. The L-lactic acid yield was also
smaller, but not significantly. Utilization of carbohydrates was complete in both cases,
and viability was better in the samples with the usual experimental design. Given
results indicate that the volume of the fermentation medium can be increased on a pilot
level, for example.",
publisher = "Технолошки факултет, Лесковац",
journal = "X SIMPOZIJUM «SAVREMENE TEHNOLOGIJE I PRIVREDNI RAZVOJ»",
title = "Lactic acid fermentation by Lactobacillus fermentum PL1 using a different volume of triticale stillage",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6201"
}

Marković, M., Markov, S., Pejin, D., Mojović, L., Pejin, J., Vukašinović-Sekulić, M.,& Đukić-Vuković, A.. (2013). Lactic acid fermentation by Lactobacillus fermentum PL1 using a different volume of triticale stillage. in X SIMPOZIJUM «SAVREMENE TEHNOLOGIJE I PRIVREDNI RAZVOJ»
Технолошки факултет, Лесковац., 86.
https://hdl.handle.net/21.15107/rcub_technorep_6201

Marković M, Markov S, Pejin D, Mojović L, Pejin J, Vukašinović-Sekulić M, Đukić-Vuković A. Lactic acid fermentation by Lactobacillus fermentum PL1 using a different volume of triticale stillage. in X SIMPOZIJUM «SAVREMENE TEHNOLOGIJE I PRIVREDNI RAZVOJ». 2013;:86.
https://hdl.handle.net/21.15107/rcub_technorep_6201 .

Marković, Milica, Markov, Siniša, Pejin, Dušanka, Mojović, Ljiljana, Pejin, Jelena, Vukašinović-Sekulić, Maja, Đukić-Vuković, Aleksandra, "Lactic acid fermentation by Lactobacillus fermentum PL1 using a different volume of triticale stillage" in X SIMPOZIJUM «SAVREMENE TEHNOLOGIJE I PRIVREDNI RAZVOJ» (2013):86,
https://hdl.handle.net/21.15107/rcub_technorep_6201 .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Pejin, Jelena
AU  - Nikolić, Svetlana
AU  - Đukić-Vuković, Aleksandra
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2186
AB  - Bioethanol accounts for the majority of biofuel use worldwide, either as a fuel or a gasoline enhancer. In Serbia, the industrial production of bioethanol still relies on conventional feedstocks containing starch and sugar such as corn, wheat and molasses. In order to improve the economy of bioethanol production and to avoid the competition of the feedstock utilization for food and energy, several production approaches based on crop selection, process integration and waste utilization were considered in this paper. Particular attention was put on utilization of non conventional crops such as triticale and damaged crops not appropriate for food consumption. Potential of lignocellulosic biomass for the production of second generation ethanol in Serbia was also considered as well as the utilization of stillage as a main by-product. The investigated approaches can significantly improve the economy of bioethanol production and contribute to solve serious environmental problems.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Renewable & Sustainable Energy Reviews
T1  - How to improve the economy of bioethanol production in Serbia
EP  - 6047
IS  - 8
SP  - 6040
VL  - 16
DO  - 10.1016/j.rser.2012.07.001
ER  - 

@article{
author = "Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Pejin, Jelena and Nikolić, Svetlana and Đukić-Vuković, Aleksandra",
year = "2012",
abstract = "Bioethanol accounts for the majority of biofuel use worldwide, either as a fuel or a gasoline enhancer. In Serbia, the industrial production of bioethanol still relies on conventional feedstocks containing starch and sugar such as corn, wheat and molasses. In order to improve the economy of bioethanol production and to avoid the competition of the feedstock utilization for food and energy, several production approaches based on crop selection, process integration and waste utilization were considered in this paper. Particular attention was put on utilization of non conventional crops such as triticale and damaged crops not appropriate for food consumption. Potential of lignocellulosic biomass for the production of second generation ethanol in Serbia was also considered as well as the utilization of stillage as a main by-product. The investigated approaches can significantly improve the economy of bioethanol production and contribute to solve serious environmental problems.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Renewable & Sustainable Energy Reviews",
title = "How to improve the economy of bioethanol production in Serbia",
pages = "6047-6040",
number = "8",
volume = "16",
doi = "10.1016/j.rser.2012.07.001"
}

Mojović, L., Pejin, D., Rakin, M., Pejin, J., Nikolić, S.,& Đukić-Vuković, A.. (2012). How to improve the economy of bioethanol production in Serbia. in Renewable & Sustainable Energy Reviews
Pergamon-Elsevier Science Ltd, Oxford., 16(8), 6040-6047.
https://doi.org/10.1016/j.rser.2012.07.001

Mojović L, Pejin D, Rakin M, Pejin J, Nikolić S, Đukić-Vuković A. How to improve the economy of bioethanol production in Serbia. in Renewable & Sustainable Energy Reviews. 2012;16(8):6040-6047.
doi:10.1016/j.rser.2012.07.001 .

Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Pejin, Jelena, Nikolić, Svetlana, Đukić-Vuković, Aleksandra, "How to improve the economy of bioethanol production in Serbia" in Renewable & Sustainable Energy Reviews, 16, no. 8 (2012):6040-6047,
https://doi.org/10.1016/j.rser.2012.07.001 . .

TY  - JOUR
AU  - Kocić-Tanackov, Sunčica
AU  - Dimić, Gordana
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Pejin, Jelena
AU  - Tanackov, Ilija
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2035
AB  - This study was aimed at investigating the antifungal potential of basil (Ocimmum basilicum L.) extract against toxin-producing Penicillium spp. (P. aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum) isolated from food. The basil extract composition was determined by the GC-MS method. The major component identified in the extract was estragole (86.72%). The determination of the antifungal activity of basil extract on Penicillium spp. was performed using the agar plate method. Basil extract reduced the growth of Penicillium spp. at all applied concentration levels (0.16, 0.35, 0.70, and 1.50 mL/100mL) with the colony growth inhibition from 3.6 (for P. glabrum) to 100% (for P. chrysogenum). The highest sensitivity showed P. chrysogenum, where the growth was completely inhibited at the basil extract concentration of 1.50 mL/100mL. The growth of other Penicillium spp. was partially inhibited with the colony growth inhibition of 63.4 % (P. brevicompactum), 67.5% (P. aurantiogriseum), and 71.7% (P. glabrum). Higher concentrations (0.70 and 1.50 mL/100mL) reduced the growth of the aerial mycelium of all tested Penicillium species. In addition, at the same extract concentrations, the examination of microscopic preparation showed the deformation of hyphae with the frequent occurrence of fragmentations and thickenings, occurrence of irregular vesicle, frequently without metulae and phialides, enlarged metulae. The results obtained in this investigation point to the possibility of using basil extract for the antifungal food protection.
AB  - Ovaj rad prikazuje antifungalni potencijal ekstrakta bosiljka na rast toksin- produkujućih Penicillium vrsta (P. aurantiogriseum, P. glabrum, P. chrysogenum i P. brevicompactum) izolovanih iz hrane. Sastav ekstrakta bosiljka određen je GC-MS metodom i u najvećem procentu sadržavao je estragol (86,72%). Uticaj ekstrakta na rast plesni ispitan je metodom agar ploča. Pri svim primenjenim koncentracija ekstrakta (0,16; 0,35; 0,70 i 1,50 mL/100mL) redukovan je rast Penicillium spp. uz inhibiciju rasta kolonija od 3,6 (P. glabrum) do 100% (P. chrysogenum). Najveću osetljivost pokazala je plesan P. chrysogenum. Njen rast je bio potpuno sprečen pri koncentraciji od 1,5 mL/100mL. Rast ostalih Penicillium spp. je delimično inhibiran sa visokim procentom inhibicije rasta kolonije od 63,4 (P. brevicompactum), 67,5 (P. aurantiogriseum) i 71,7% (P. glabrum). Više koncentracije ekstrakta (0,70 i 1,5 mL/100mL) su redukovale micelarni rast svih testiranih vrsta. Takođe, pri ovim koncentracijama su u mikroskopskom preparatu uočene deformacije hifa sa čestom fragmentacijom i zadebljanjima, vezikule nepravilnog oblika, pojava proširenih metula, ali često bez metula i fijalida. Dobijeni rezultati ukazuju na mogućnost korišćenja ekstrakta bosiljka u antifungalnoj zaštiti hrane.
PB  - Faculty of Technology, Novi Sad
T2  - Acta periodica technologica
T1  - Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum
T1  - Antifungalna aktivnost ekstrakta bosiljka (Ocimmum basilicum L.) na rast Penicillium aurantiogriseum, P. glabrum, P. chrysogenum i P. brevicompactum
EP  - 256
IS  - 43
SP  - 247
DO  - 10.2298/APT1243247K
ER  - 

@article{
author = "Kocić-Tanackov, Sunčica and Dimić, Gordana and Pejin, Dušanka and Mojović, Ljiljana and Pejin, Jelena and Tanackov, Ilija",
year = "2012",
abstract = "This study was aimed at investigating the antifungal potential of basil (Ocimmum basilicum L.) extract against toxin-producing Penicillium spp. (P. aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum) isolated from food. The basil extract composition was determined by the GC-MS method. The major component identified in the extract was estragole (86.72%). The determination of the antifungal activity of basil extract on Penicillium spp. was performed using the agar plate method. Basil extract reduced the growth of Penicillium spp. at all applied concentration levels (0.16, 0.35, 0.70, and 1.50 mL/100mL) with the colony growth inhibition from 3.6 (for P. glabrum) to 100% (for P. chrysogenum). The highest sensitivity showed P. chrysogenum, where the growth was completely inhibited at the basil extract concentration of 1.50 mL/100mL. The growth of other Penicillium spp. was partially inhibited with the colony growth inhibition of 63.4 % (P. brevicompactum), 67.5% (P. aurantiogriseum), and 71.7% (P. glabrum). Higher concentrations (0.70 and 1.50 mL/100mL) reduced the growth of the aerial mycelium of all tested Penicillium species. In addition, at the same extract concentrations, the examination of microscopic preparation showed the deformation of hyphae with the frequent occurrence of fragmentations and thickenings, occurrence of irregular vesicle, frequently without metulae and phialides, enlarged metulae. The results obtained in this investigation point to the possibility of using basil extract for the antifungal food protection., Ovaj rad prikazuje antifungalni potencijal ekstrakta bosiljka na rast toksin- produkujućih Penicillium vrsta (P. aurantiogriseum, P. glabrum, P. chrysogenum i P. brevicompactum) izolovanih iz hrane. Sastav ekstrakta bosiljka određen je GC-MS metodom i u najvećem procentu sadržavao je estragol (86,72%). Uticaj ekstrakta na rast plesni ispitan je metodom agar ploča. Pri svim primenjenim koncentracija ekstrakta (0,16; 0,35; 0,70 i 1,50 mL/100mL) redukovan je rast Penicillium spp. uz inhibiciju rasta kolonija od 3,6 (P. glabrum) do 100% (P. chrysogenum). Najveću osetljivost pokazala je plesan P. chrysogenum. Njen rast je bio potpuno sprečen pri koncentraciji od 1,5 mL/100mL. Rast ostalih Penicillium spp. je delimično inhibiran sa visokim procentom inhibicije rasta kolonije od 63,4 (P. brevicompactum), 67,5 (P. aurantiogriseum) i 71,7% (P. glabrum). Više koncentracije ekstrakta (0,70 i 1,5 mL/100mL) su redukovale micelarni rast svih testiranih vrsta. Takođe, pri ovim koncentracijama su u mikroskopskom preparatu uočene deformacije hifa sa čestom fragmentacijom i zadebljanjima, vezikule nepravilnog oblika, pojava proširenih metula, ali često bez metula i fijalida. Dobijeni rezultati ukazuju na mogućnost korišćenja ekstrakta bosiljka u antifungalnoj zaštiti hrane.",
publisher = "Faculty of Technology, Novi Sad",
journal = "Acta periodica technologica",
title = "Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum, Antifungalna aktivnost ekstrakta bosiljka (Ocimmum basilicum L.) na rast Penicillium aurantiogriseum, P. glabrum, P. chrysogenum i P. brevicompactum",
pages = "256-247",
number = "43",
doi = "10.2298/APT1243247K"
}

Kocić-Tanackov, S., Dimić, G., Pejin, D., Mojović, L., Pejin, J.,& Tanackov, I.. (2012). Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum. in Acta periodica technologica
Faculty of Technology, Novi Sad.(43), 247-256.
https://doi.org/10.2298/APT1243247K

Kocić-Tanackov S, Dimić G, Pejin D, Mojović L, Pejin J, Tanackov I. Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum. in Acta periodica technologica. 2012;(43):247-256.
doi:10.2298/APT1243247K .

Kocić-Tanackov, Sunčica, Dimić, Gordana, Pejin, Dušanka, Mojović, Ljiljana, Pejin, Jelena, Tanackov, Ilija, "Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum" in Acta periodica technologica, no. 43 (2012):247-256,
https://doi.org/10.2298/APT1243247K . .

TY  - JOUR
AU  - Kocić-Tanackov, Sunčica
AU  - Dimić, Gordana
AU  - Tanackov, Ilija
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Pejin, Jelena
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2102
AB  - The effect of the oregano extract (Origanum vulgare L.) on the growth of Fusarium and Penicillium species isolated from cakes and ready-for-use fresh salads from different kinds of vegetables was investigated. The contents of the active component of extract were identified by GC-MS and they include: carvacrol (34.2%), carvone (18.5%), p-cimene (8.05%), thymol (3.74%). The oregano extract showed the ability to reduce mould growth at all applied concentrations. Stronger inhibitory effect on the growth of Penicillium species, contrary to Fusarium, was determined. At extract concentration of 2.50 mL/100 mL, growth of P. aurantiogriseum, P. glabrum and P. brevicompactum was completely inhibited during 14 days of incubation. At the same concentration, growth of Fusarium proliferatum was inhibited by 81.71%, F. oxysporum by 85.84%, F. verticillioides by 86.50%, P. chrysogenum by 86.2% and F. subglutinans by 88.85%.
AB  - U radu je prikazan uticaj ekstrakta origana (Origanum vulgare L.) na rast Fusarium i Penicillium vrsta izolovanih iz kolača i salata od svežeg povrća. Antifungalna ispitivanja su izvedena metodom agar ploča. Sastav aktivnih komponenti ekstrakta je određen GC-MS metodom i kao glavne komponente ustanovljene su: karvakrol (34,2%), karvon (18,5%) i p-cimen (8,05%). Ispitivani ekstrakt je na svim primenjenim koncentracijama pokazao sposobnost redukcije rasta plesni. Jači inhibitorni efekat je utvrđen na rast Penicillium vrsta. Pri koncentraciji od 2,50 mL/100 mL ekstrakta rast P. aurantiogriseum, P. glabrum i P. brevicompactum je kompletno inhibiran tokom 14 dana inkubiranja. Pri istoj koncentraciji rast F. proliferatum je inhibiran za 81,71%, F. oxysporum za 85,84%, F. verticillioides za 86,50%, P. chrysogenum za 86,2% i F. subglutinans za 88,85%. Ispitivani začinski ekstrakt origana je pored ograničavanja rasta kolonija plesni uzrokovao i promene u makro- i mikromorfologiji. Pri višim koncentracijama (1,50 i 2,50 mL/100 mL) došlo je do formiranja nekarakterističnih, kolonija sa izdignutim centrom, zrnaste strukture i smanjenom konidijacijom (P. brevicompactum, P. chrysogenum, F. oxysporum). Takođe, na ovim koncentracijama su u mikroskopskom preparatu uočene deformacije hifa (sa čestom fragmentacijom i zadebljanjima, ugibanje) i reproduktivnih organa (vezikule nepravilnog oblika, često bez metula i fijalida, pojava proširenih metula, deformacija monofijalida) ili bez reprouktivnih organa (P. aurantiogriseum, P. brevicompactum i P. chrysogenum). Rezultati pokazuju da bi ispitivani ekstrat origana mogao imati zaštitni efekat u kontroli rasta plesni u hrani tokom čuvanja ili čak produžiti rok trajnosti hrane.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Antifungal activity of oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food
T1  - Antifungalna aktivnost ekstrakta origana (Origanum vulgare L.) na rast Fusarium i Penicillium vrsta izolovanih iz hrane
EP  - 41
IS  - 1
SP  - 33
VL  - 66
DO  - 10.2298/HEMIND110614073K
ER  - 

@article{
author = "Kocić-Tanackov, Sunčica and Dimić, Gordana and Tanackov, Ilija and Pejin, Dušanka and Mojović, Ljiljana and Pejin, Jelena",
year = "2012",
abstract = "The effect of the oregano extract (Origanum vulgare L.) on the growth of Fusarium and Penicillium species isolated from cakes and ready-for-use fresh salads from different kinds of vegetables was investigated. The contents of the active component of extract were identified by GC-MS and they include: carvacrol (34.2%), carvone (18.5%), p-cimene (8.05%), thymol (3.74%). The oregano extract showed the ability to reduce mould growth at all applied concentrations. Stronger inhibitory effect on the growth of Penicillium species, contrary to Fusarium, was determined. At extract concentration of 2.50 mL/100 mL, growth of P. aurantiogriseum, P. glabrum and P. brevicompactum was completely inhibited during 14 days of incubation. At the same concentration, growth of Fusarium proliferatum was inhibited by 81.71%, F. oxysporum by 85.84%, F. verticillioides by 86.50%, P. chrysogenum by 86.2% and F. subglutinans by 88.85%., U radu je prikazan uticaj ekstrakta origana (Origanum vulgare L.) na rast Fusarium i Penicillium vrsta izolovanih iz kolača i salata od svežeg povrća. Antifungalna ispitivanja su izvedena metodom agar ploča. Sastav aktivnih komponenti ekstrakta je određen GC-MS metodom i kao glavne komponente ustanovljene su: karvakrol (34,2%), karvon (18,5%) i p-cimen (8,05%). Ispitivani ekstrakt je na svim primenjenim koncentracijama pokazao sposobnost redukcije rasta plesni. Jači inhibitorni efekat je utvrđen na rast Penicillium vrsta. Pri koncentraciji od 2,50 mL/100 mL ekstrakta rast P. aurantiogriseum, P. glabrum i P. brevicompactum je kompletno inhibiran tokom 14 dana inkubiranja. Pri istoj koncentraciji rast F. proliferatum je inhibiran za 81,71%, F. oxysporum za 85,84%, F. verticillioides za 86,50%, P. chrysogenum za 86,2% i F. subglutinans za 88,85%. Ispitivani začinski ekstrakt origana je pored ograničavanja rasta kolonija plesni uzrokovao i promene u makro- i mikromorfologiji. Pri višim koncentracijama (1,50 i 2,50 mL/100 mL) došlo je do formiranja nekarakterističnih, kolonija sa izdignutim centrom, zrnaste strukture i smanjenom konidijacijom (P. brevicompactum, P. chrysogenum, F. oxysporum). Takođe, na ovim koncentracijama su u mikroskopskom preparatu uočene deformacije hifa (sa čestom fragmentacijom i zadebljanjima, ugibanje) i reproduktivnih organa (vezikule nepravilnog oblika, često bez metula i fijalida, pojava proširenih metula, deformacija monofijalida) ili bez reprouktivnih organa (P. aurantiogriseum, P. brevicompactum i P. chrysogenum). Rezultati pokazuju da bi ispitivani ekstrat origana mogao imati zaštitni efekat u kontroli rasta plesni u hrani tokom čuvanja ili čak produžiti rok trajnosti hrane.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Antifungal activity of oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food, Antifungalna aktivnost ekstrakta origana (Origanum vulgare L.) na rast Fusarium i Penicillium vrsta izolovanih iz hrane",
pages = "41-33",
number = "1",
volume = "66",
doi = "10.2298/HEMIND110614073K"
}

Kocić-Tanackov, S., Dimić, G., Tanackov, I., Pejin, D., Mojović, L.,& Pejin, J.. (2012). Antifungal activity of oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food. in Hemijska industrija
Association of Chemical Engineers of Serbia., 66(1), 33-41.
https://doi.org/10.2298/HEMIND110614073K

Kocić-Tanackov S, Dimić G, Tanackov I, Pejin D, Mojović L, Pejin J. Antifungal activity of oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food. in Hemijska industrija. 2012;66(1):33-41.
doi:10.2298/HEMIND110614073K .

Kocić-Tanackov, Sunčica, Dimić, Gordana, Tanackov, Ilija, Pejin, Dušanka, Mojović, Ljiljana, Pejin, Jelena, "Antifungal activity of oregano (Origanum vulgare L.) extract on the growth of Fusarium and Penicillium species isolated from food" in Hemijska industrija, 66, no. 1 (2012):33-41,
https://doi.org/10.2298/HEMIND110614073K . .

TY  - JOUR
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Pejin, Jelena
AU  - Grujić, Olgica
AU  - Markov, Siniša
AU  - Nikolić, Svetlana
AU  - Marković, Milica N.
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2218
AB  - BACKGROUND: Bioethanol produced from renewable biomass, such as sugar, starch or lignocellulosic materials, is one of the alternative energy resources that is environmentally friendly. Triticale crops have a high yield as well as a high starch content and amylolytic enzyme activity and are therefore considered to be ideal for bioethanol production. RESULTS: This study examined the feasibility of ultrasound pretreatment to enhance the release of fermentable sugars from triticale meal during pretreatment and consequently increase bioethanol yield in the simultaneous saccharification and fermentation (SSF) process by Saccharomyces cerevisiae yeast. Ultrasonic pretreatment effectively increased the glucose and maltose content after liquefaction by 15.71% and 52.57%, respectively, compared with the untreated control sample under determined optimal conditions of sonication (5 min, 60 degrees C). The ultrasound pretreatment consequently improved bioethanol production during SSF processing since the bioethanol content was increased by 10.89%. CONCLUSION: Taking into consideration significant process parameters obtained in the SSF process of triticale meal with ultrasound pretreatment at 60 degrees C, the process time may be reduced from 72 to 48 h. At that point of the SSF, maximum bioethanol content of 9.55% (w/v), bioethanol yield of 0.43 g g(-1) of triticale starch, and percentage of the theoretical bioethanol yield of 84.56% were achieved.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Increase in bioethanol production yield from triticale by simultaneous saccharification and fermentation with application of ultrasound
EP  - 176
IS  - 2
SP  - 170
VL  - 87
DO  - 10.1002/jctb.2675
ER  - 

@article{
author = "Pejin, Dušanka and Mojović, Ljiljana and Pejin, Jelena and Grujić, Olgica and Markov, Siniša and Nikolić, Svetlana and Marković, Milica N.",
year = "2012",
abstract = "BACKGROUND: Bioethanol produced from renewable biomass, such as sugar, starch or lignocellulosic materials, is one of the alternative energy resources that is environmentally friendly. Triticale crops have a high yield as well as a high starch content and amylolytic enzyme activity and are therefore considered to be ideal for bioethanol production. RESULTS: This study examined the feasibility of ultrasound pretreatment to enhance the release of fermentable sugars from triticale meal during pretreatment and consequently increase bioethanol yield in the simultaneous saccharification and fermentation (SSF) process by Saccharomyces cerevisiae yeast. Ultrasonic pretreatment effectively increased the glucose and maltose content after liquefaction by 15.71% and 52.57%, respectively, compared with the untreated control sample under determined optimal conditions of sonication (5 min, 60 degrees C). The ultrasound pretreatment consequently improved bioethanol production during SSF processing since the bioethanol content was increased by 10.89%. CONCLUSION: Taking into consideration significant process parameters obtained in the SSF process of triticale meal with ultrasound pretreatment at 60 degrees C, the process time may be reduced from 72 to 48 h. At that point of the SSF, maximum bioethanol content of 9.55% (w/v), bioethanol yield of 0.43 g g(-1) of triticale starch, and percentage of the theoretical bioethanol yield of 84.56% were achieved.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Increase in bioethanol production yield from triticale by simultaneous saccharification and fermentation with application of ultrasound",
pages = "176-170",
number = "2",
volume = "87",
doi = "10.1002/jctb.2675"
}

Pejin, D., Mojović, L., Pejin, J., Grujić, O., Markov, S., Nikolić, S.,& Marković, M. N.. (2012). Increase in bioethanol production yield from triticale by simultaneous saccharification and fermentation with application of ultrasound. in Journal of Chemical Technology and Biotechnology
Wiley-Blackwell, Hoboken., 87(2), 170-176.
https://doi.org/10.1002/jctb.2675

Pejin D, Mojović L, Pejin J, Grujić O, Markov S, Nikolić S, Marković MN. Increase in bioethanol production yield from triticale by simultaneous saccharification and fermentation with application of ultrasound. in Journal of Chemical Technology and Biotechnology. 2012;87(2):170-176.
doi:10.1002/jctb.2675 .

Pejin, Dušanka, Mojović, Ljiljana, Pejin, Jelena, Grujić, Olgica, Markov, Siniša, Nikolić, Svetlana, Marković, Milica N., "Increase in bioethanol production yield from triticale by simultaneous saccharification and fermentation with application of ultrasound" in Journal of Chemical Technology and Biotechnology, 87, no. 2 (2012):170-176,
https://doi.org/10.1002/jctb.2675 . .

TY  - JOUR
AU  - Kocić-Tanackov, Sunčica
AU  - Dimić, Gordana
AU  - Tanackov, Ilija
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Pejin, Jelena
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2166
AB  - The aim of this study was to investigate the effect of oregano extract on the growth of Aspergillus species (Aspergillus niger, Aspergillus carbonarius, and Aspergillus wentii) isolated from food and biosynthesis of sterigmatocystin (SIC) by Aspergillus versicolor. Antifungal determinations were conducted using the agar plate method. The effect of oregano extract on the biosynthesis of STC was determined in a Yeast Extract Sucrose (YES) broth. The SIC content and mycelial growth of A. versicolor were determined on the 7th, 14th, and 21st day with all applied concentrations of oregano extract and control sample. The composition of oregano extract was determined by gas chromatography-mass spectrometry (GC-MS) and 21 different components were identified. The major components were carvacrol (34.20%) and carvone (18.05%). At 2.5 mL/100 mL concentration, the oregano extract completely inhibited the growth of A. wentii, while the growth of A. carbonarius and A. niger was reduced by 95.6% and 45.6%, respectively. Significant reductions in SIC biosynthesis during 21 days of incubation were observed for extract concentrations higher than 0.06 mL/100 mL. The obtained results showed that the tested oregano extract could be used as a food preservative to prevent food-borne fungal infections and mycotoxin production.
PB  - Elsevier, Amsterdam
T2  - LWT-Food Science and Technology
T1  - The inhibitory effect of oregano extract on the growth of Aspergillus spp. and on sterigmatocystin biosynthesis
EP  - 20
IS  - 1
SP  - 14
VL  - 49
DO  - 10.1016/j.lwt.2012.04.013
ER  - 

@article{
author = "Kocić-Tanackov, Sunčica and Dimić, Gordana and Tanackov, Ilija and Pejin, Dušanka and Mojović, Ljiljana and Pejin, Jelena",
year = "2012",
abstract = "The aim of this study was to investigate the effect of oregano extract on the growth of Aspergillus species (Aspergillus niger, Aspergillus carbonarius, and Aspergillus wentii) isolated from food and biosynthesis of sterigmatocystin (SIC) by Aspergillus versicolor. Antifungal determinations were conducted using the agar plate method. The effect of oregano extract on the biosynthesis of STC was determined in a Yeast Extract Sucrose (YES) broth. The SIC content and mycelial growth of A. versicolor were determined on the 7th, 14th, and 21st day with all applied concentrations of oregano extract and control sample. The composition of oregano extract was determined by gas chromatography-mass spectrometry (GC-MS) and 21 different components were identified. The major components were carvacrol (34.20%) and carvone (18.05%). At 2.5 mL/100 mL concentration, the oregano extract completely inhibited the growth of A. wentii, while the growth of A. carbonarius and A. niger was reduced by 95.6% and 45.6%, respectively. Significant reductions in SIC biosynthesis during 21 days of incubation were observed for extract concentrations higher than 0.06 mL/100 mL. The obtained results showed that the tested oregano extract could be used as a food preservative to prevent food-borne fungal infections and mycotoxin production.",
publisher = "Elsevier, Amsterdam",
journal = "LWT-Food Science and Technology",
title = "The inhibitory effect of oregano extract on the growth of Aspergillus spp. and on sterigmatocystin biosynthesis",
pages = "20-14",
number = "1",
volume = "49",
doi = "10.1016/j.lwt.2012.04.013"
}

Kocić-Tanackov, S., Dimić, G., Tanackov, I., Pejin, D., Mojović, L.,& Pejin, J.. (2012). The inhibitory effect of oregano extract on the growth of Aspergillus spp. and on sterigmatocystin biosynthesis. in LWT-Food Science and Technology
Elsevier, Amsterdam., 49(1), 14-20.
https://doi.org/10.1016/j.lwt.2012.04.013

Kocić-Tanackov S, Dimić G, Tanackov I, Pejin D, Mojović L, Pejin J. The inhibitory effect of oregano extract on the growth of Aspergillus spp. and on sterigmatocystin biosynthesis. in LWT-Food Science and Technology. 2012;49(1):14-20.
doi:10.1016/j.lwt.2012.04.013 .

Kocić-Tanackov, Sunčica, Dimić, Gordana, Tanackov, Ilija, Pejin, Dušanka, Mojović, Ljiljana, Pejin, Jelena, "The inhibitory effect of oregano extract on the growth of Aspergillus spp. and on sterigmatocystin biosynthesis" in LWT-Food Science and Technology, 49, no. 1 (2012):14-20,
https://doi.org/10.1016/j.lwt.2012.04.013 . .

TY  - CONF
AU  - Đukić Vuković, Aleksandra
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Pejin, Jelena
AU  - Bulatović, Maja
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7371
AB  - In Serbia, the industrial production of bioethanol still relies on conventional energy crops (corn, wheat triticale and molasses). The production process generates large amounts of waste liquid stillage (app. 13 hL per hL of bioethanol produced) with high BOD, values which may cause serious environmental problems if not treated adequately. The aim of this paper is to provide an overview of the possibilities of stillage utilization from bioethanol production on starch based feedstocks. Three main approaches were analyzed and presented: (1) recirculation of liquid corn stillage in mashing process; (2) employment of solid corn stillage remained after ethanol distillation as animal feed; (3) utilization of three different liquid stillages for the production of lactic acid. It has been shown that the bioethanol yield can be significantly increased by the recirculation of liquid corn stillage in the amount from 10 to 30%, while the solid fraction can be used as a high quality feed rich in proteins and minerals. In addition, very high values of lactic acid yields were accomplished during lactic acid production on three different liquid stillages by Lactobacillus genera. All of the investigated approaches can significantly improve the economy of bioethanol production on starch grains.
PB  - SaySoft Inc., Italy
C3  - Book of Proceedings / XIX International Symposium on Alcohol Fuels (ISAF XIX), Development and utilisation of alchohol fuels, to promote sustainability, October 10-14, 2011, Verona, Italy
T1  - The possibilities of utilization of stillage from the production of bioethanol on starch feedstocks
EP  - 419
SP  - 415
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7371
ER  - 

@conference{
author = "Đukić Vuković, Aleksandra and Nikolić, Svetlana and Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Pejin, Jelena and Bulatović, Maja",
year = "2011",
abstract = "In Serbia, the industrial production of bioethanol still relies on conventional energy crops (corn, wheat triticale and molasses). The production process generates large amounts of waste liquid stillage (app. 13 hL per hL of bioethanol produced) with high BOD, values which may cause serious environmental problems if not treated adequately. The aim of this paper is to provide an overview of the possibilities of stillage utilization from bioethanol production on starch based feedstocks. Three main approaches were analyzed and presented: (1) recirculation of liquid corn stillage in mashing process; (2) employment of solid corn stillage remained after ethanol distillation as animal feed; (3) utilization of three different liquid stillages for the production of lactic acid. It has been shown that the bioethanol yield can be significantly increased by the recirculation of liquid corn stillage in the amount from 10 to 30%, while the solid fraction can be used as a high quality feed rich in proteins and minerals. In addition, very high values of lactic acid yields were accomplished during lactic acid production on three different liquid stillages by Lactobacillus genera. All of the investigated approaches can significantly improve the economy of bioethanol production on starch grains.",
publisher = "SaySoft Inc., Italy",
journal = "Book of Proceedings / XIX International Symposium on Alcohol Fuels (ISAF XIX), Development and utilisation of alchohol fuels, to promote sustainability, October 10-14, 2011, Verona, Italy",
title = "The possibilities of utilization of stillage from the production of bioethanol on starch feedstocks",
pages = "419-415",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7371"
}

Đukić Vuković, A., Nikolić, S., Mojović, L., Pejin, D., Rakin, M., Pejin, J.,& Bulatović, M.. (2011). The possibilities of utilization of stillage from the production of bioethanol on starch feedstocks. in Book of Proceedings / XIX International Symposium on Alcohol Fuels (ISAF XIX), Development and utilisation of alchohol fuels, to promote sustainability, October 10-14, 2011, Verona, Italy
SaySoft Inc., Italy., 415-419.
https://hdl.handle.net/21.15107/rcub_technorep_7371

Đukić Vuković A, Nikolić S, Mojović L, Pejin D, Rakin M, Pejin J, Bulatović M. The possibilities of utilization of stillage from the production of bioethanol on starch feedstocks. in Book of Proceedings / XIX International Symposium on Alcohol Fuels (ISAF XIX), Development and utilisation of alchohol fuels, to promote sustainability, October 10-14, 2011, Verona, Italy. 2011;:415-419.
https://hdl.handle.net/21.15107/rcub_technorep_7371 .

Đukić Vuković, Aleksandra, Nikolić, Svetlana, Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Pejin, Jelena, Bulatović, Maja, "The possibilities of utilization of stillage from the production of bioethanol on starch feedstocks" in Book of Proceedings / XIX International Symposium on Alcohol Fuels (ISAF XIX), Development and utilisation of alchohol fuels, to promote sustainability, October 10-14, 2011, Verona, Italy (2011):415-419,
https://hdl.handle.net/21.15107/rcub_technorep_7371 .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Vukašinović-Sekulić, Maja
AU  - Đukić-Vuković, Aleksandra
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Pejin, Jelena
AU  - Nikolić, Svetlana
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1834
AB  - Bioethanol produced by fermentation of biomass is one of the most important renewable and ecologically friendly energy resources today. After bioethanol distillation a considerable amount of liquid stillage remains (1 hl of the produced bioethanol, approximately 13 hl of liquid thin stillage is formed). Since the stillage contains high concentrations of organic and inorganic substances, it may cause serious environmental problems if it is disposed to water flows untreated. The aim of this work was to investigate potential of application of corn thin stillage as an inexpensive renewable feedstock for lactic acid production. In this way, it is possible to solve ecological problems and to improve the economy of bioethanol production taking into account growing demands for lactic acid for utilization in pharmaceutical, food, chemical, textile and leader industry. Lactic acid fermentation was conducted with nine different species from genera Lactobacillus and their growth, sugar utilization and lactic acid production on corn thin stillage were compared. The most productive strains were two facultatively heterofermentative species Lb. paracasei ssp. paracasei NRRL B-4564 and Lb. casei ssp. casei NRRL B-441, which had almost identical kinetics of lactic acid production during the first 48 hours, and after that the rate of lactic acid formation decreased more for strain Lb. casei ssp. casei NRRL B-441. For this reason the species Lb. paracasei ssp. paracasei NRRL B-4564 was selected for further study of effect of temperature, oxygen and shaking on lactic acid production. The lactic acid production was maximal at 41°C, without shaking, under anaerobic conditions. However, the biomass production was better at 30°C, also without shaking, under anaerobic conditions.
AB  - Bioetanol koji se proizvodi fermentacijom biomase predstavlja izuzetno značajno i ekološki pogodno biogorivo čija se proizvodnja danas konstantno povećava. Nakon proizvodnje bioetanola zaostaje značajna količina džibre kao otpadnog proizvoda (na 1 hl proizvedenog etanola nastaje oko 13 hl tečne džibre). Zbog prisustva značajnih količina organskih i neorganskih jedinjenja ispuštanje tečne džibre u vodene tokove može prouzrokovati ozbiljna ekološka zagađenja. Cilj ovog rada je da se ispita mogućnost korišćenja tečne džibre iz proizvodnje bioetanola na kukuruzu kao sirovine za proizvodnju mlečne kiseline. Na ovaj način je moguće rešiti problem otpada i značajno povećati ekonomičnost procesa proizvodnje bioetanola, imajući u vidu rastući trend u proizvodnji mlečne kiseline i njenu sve veću primenu u farmaceutskoj, hemijskoj, prehrambenoj, tekstilnoj i kožnoj industriji. U radu je ispitana mlečno kiselinska fermentacija devet vrsta bakterija iz roda Lactobacillus u toku koje je praćen njihov rast, iskorišćenje šećera iz supstrata (tečne džibre) i koncentracija mlečne kiseline. Najbolja aktivnost uočena je kod fakultativno heterofermentativnih vrsta Lb. paracasei ssp. paracasei NRRL B-4564 i Lb. casei ssp. casei NRRL B-441, kod kojih je zapažen gotovo identičan tok mlečne fermentacije u toku prvih 48 h, nakon čega brzina obrazovanja mlečne kiseline brže opada kod vrste Lb. casei ssp. casei NRRL B-441. Zbog toga je za dalji rad izabrana vrsta Lb. paracasei ssp. paracasei NRRL B-4564 sa kojom je ispitan uticaj temperature, kiseonika i mešanja na proizvodnju mlečne kiseline. Najveći sadržaj mlečne kiseline je postignut u fermentaciji na 41°C, u statičnim, anaerobnim uslovima. Proizvodnja biomase je bila bolja pri temperaturi od 30°C, takođe bez mešanja, pod anaerobnim uslovima.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Production of lactic acid on liquid distillery stillage
T1  - Proizvodnja mlečne kiseline na tečnoj destilerijskoj džibri
EP  - 5
IS  - 1
SP  - 1
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1834
ER  - 

@article{
author = "Mojović, Ljiljana and Vukašinović-Sekulić, Maja and Đukić-Vuković, Aleksandra and Pejin, Dušanka and Rakin, Marica and Pejin, Jelena and Nikolić, Svetlana",
year = "2011",
abstract = "Bioethanol produced by fermentation of biomass is one of the most important renewable and ecologically friendly energy resources today. After bioethanol distillation a considerable amount of liquid stillage remains (1 hl of the produced bioethanol, approximately 13 hl of liquid thin stillage is formed). Since the stillage contains high concentrations of organic and inorganic substances, it may cause serious environmental problems if it is disposed to water flows untreated. The aim of this work was to investigate potential of application of corn thin stillage as an inexpensive renewable feedstock for lactic acid production. In this way, it is possible to solve ecological problems and to improve the economy of bioethanol production taking into account growing demands for lactic acid for utilization in pharmaceutical, food, chemical, textile and leader industry. Lactic acid fermentation was conducted with nine different species from genera Lactobacillus and their growth, sugar utilization and lactic acid production on corn thin stillage were compared. The most productive strains were two facultatively heterofermentative species Lb. paracasei ssp. paracasei NRRL B-4564 and Lb. casei ssp. casei NRRL B-441, which had almost identical kinetics of lactic acid production during the first 48 hours, and after that the rate of lactic acid formation decreased more for strain Lb. casei ssp. casei NRRL B-441. For this reason the species Lb. paracasei ssp. paracasei NRRL B-4564 was selected for further study of effect of temperature, oxygen and shaking on lactic acid production. The lactic acid production was maximal at 41°C, without shaking, under anaerobic conditions. However, the biomass production was better at 30°C, also without shaking, under anaerobic conditions., Bioetanol koji se proizvodi fermentacijom biomase predstavlja izuzetno značajno i ekološki pogodno biogorivo čija se proizvodnja danas konstantno povećava. Nakon proizvodnje bioetanola zaostaje značajna količina džibre kao otpadnog proizvoda (na 1 hl proizvedenog etanola nastaje oko 13 hl tečne džibre). Zbog prisustva značajnih količina organskih i neorganskih jedinjenja ispuštanje tečne džibre u vodene tokove može prouzrokovati ozbiljna ekološka zagađenja. Cilj ovog rada je da se ispita mogućnost korišćenja tečne džibre iz proizvodnje bioetanola na kukuruzu kao sirovine za proizvodnju mlečne kiseline. Na ovaj način je moguće rešiti problem otpada i značajno povećati ekonomičnost procesa proizvodnje bioetanola, imajući u vidu rastući trend u proizvodnji mlečne kiseline i njenu sve veću primenu u farmaceutskoj, hemijskoj, prehrambenoj, tekstilnoj i kožnoj industriji. U radu je ispitana mlečno kiselinska fermentacija devet vrsta bakterija iz roda Lactobacillus u toku koje je praćen njihov rast, iskorišćenje šećera iz supstrata (tečne džibre) i koncentracija mlečne kiseline. Najbolja aktivnost uočena je kod fakultativno heterofermentativnih vrsta Lb. paracasei ssp. paracasei NRRL B-4564 i Lb. casei ssp. casei NRRL B-441, kod kojih je zapažen gotovo identičan tok mlečne fermentacije u toku prvih 48 h, nakon čega brzina obrazovanja mlečne kiseline brže opada kod vrste Lb. casei ssp. casei NRRL B-441. Zbog toga je za dalji rad izabrana vrsta Lb. paracasei ssp. paracasei NRRL B-4564 sa kojom je ispitan uticaj temperature, kiseonika i mešanja na proizvodnju mlečne kiseline. Najveći sadržaj mlečne kiseline je postignut u fermentaciji na 41°C, u statičnim, anaerobnim uslovima. Proizvodnja biomase je bila bolja pri temperaturi od 30°C, takođe bez mešanja, pod anaerobnim uslovima.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Production of lactic acid on liquid distillery stillage, Proizvodnja mlečne kiseline na tečnoj destilerijskoj džibri",
pages = "5-1",
number = "1",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1834"
}

Mojović, L., Vukašinović-Sekulić, M., Đukić-Vuković, A., Pejin, D., Rakin, M., Pejin, J.,& Nikolić, S.. (2011). Production of lactic acid on liquid distillery stillage. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 15(1), 1-5.
https://hdl.handle.net/21.15107/rcub_technorep_1834

Mojović L, Vukašinović-Sekulić M, Đukić-Vuković A, Pejin D, Rakin M, Pejin J, Nikolić S. Production of lactic acid on liquid distillery stillage. in Journal on Processing and Energy in Agriculture. 2011;15(1):1-5.
https://hdl.handle.net/21.15107/rcub_technorep_1834 .

Mojović, Ljiljana, Vukašinović-Sekulić, Maja, Đukić-Vuković, Aleksandra, Pejin, Dušanka, Rakin, Marica, Pejin, Jelena, Nikolić, Svetlana, "Production of lactic acid on liquid distillery stillage" in Journal on Processing and Energy in Agriculture, 15, no. 1 (2011):1-5,
https://hdl.handle.net/21.15107/rcub_technorep_1834 .

TY  - JOUR
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Vukašinović-Sekulić, Maja
AU  - Rakin, Marica
AU  - Nikolić, Svetlana
AU  - Pejin, Jelena
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1817
AB  - The production of lactic acid on a liquid distillery stillage remaining after the bioethanol production on a mixture of waste bread and waste water from the production of wheat gluten was studied in this work. The lactic acid fermentation was performed with a probiotic lactic acid bacteria Lactobacillus rhamnosus ATCC 7469. During the fermentation, parameters such as the concentration of lactic acid (according to Taylor method), the concentration of reducing sugars (spectrophotometric method according to Miller), pH, and the bacterial growth (counting method on agar plates) were followed. The optimal conditions for the production of lactic acid such as: temperature, inoculum concentration, concentration of added CaCO3 for neutralization and shaking intensity were determined. Under these conditions, a yield of over 80% of the theoretical yield was achieved. The highest intensity of the lactic acid production was noticed during the first 24 hours of fermentation, while the fermentation completely ended after 72 hours. An intensive growth of the L. rhamnosus ATCC 7469 was detected under the optimal conditions, indicating a possibility of the parallel production of lactic acid and probiotic biomass. This approach could be economically more favorable. The liquid stillage used in this study was shown as a good substrate for lactic acid fermentation.
AB  - U radu je ispitivana proizvodnja mlečne kiseline na tečnoj destilerijskoj džibri nastaloj nakon proizvodnje bioetanola iz smeše otpadnog hleba i otpadne vode iz proizvodnje glutena. Mlečno-kiselinska fermentacija je vršena pomoću probiotički aktivne bakterije Lactobacillus rhamnosus ATCC 7469. U toku fermentacije praćen je sadržaj mlečne kiseline (po metodi Taylor-a), sadržaj redukujućih šećera (spektrofotometrijski metodom po Miller-u), pH, kao i rast bakterije (brojanjem ćelija Koh-ovom metodom na agarnim pločama). Utvrđeni su optimalni uslovi za proizvodnju mlečne kiseline: temperatura, koncentracija inokuluma, koncentracija dodatog CaCO3 kao sredstva za neutralizaciju i intenzitet mešanja. Pri utvrđenim optimalnim uslovima ostvareni su prinosi od preko 80% od teorijskog prinosa mlečne kiseline u mikroaerofilnoj fermentaciji sa mešanjem. Najveći intezitet fermentacije ostvaren je u toku prvih 24 časa, a fermentacija je završena nakon 72 časa. Pri optimalnim uslovima mlečnokiselinske fermentacije uočen je intenzivan rast bakterije Lactobacillus rhamnosus ATCC 7469 što ukazuje na mogućnost paralelne proizvodnje mlečne kiseline i probiotičke biomase, čime bi se mogli postići bolji ekonomski efekti. Ispitivana džibra se pokazala kao pogodan medijum za mlečno kiselinsku fermentaciju.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - The production of lactic acid on liquid distillery stillage by Lactobacillus rhamnosus ATCC 7469
T1  - Proizvodnja mlečne kiseline na tečnoj destilerijskoj džibri pomoću Lactobacillus rhamnosus ATCC 7469
EP  - 104
IS  - 20
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1817
ER  - 

@article{
author = "Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Pejin, Dušanka and Vukašinović-Sekulić, Maja and Rakin, Marica and Nikolić, Svetlana and Pejin, Jelena",
year = "2011",
abstract = "The production of lactic acid on a liquid distillery stillage remaining after the bioethanol production on a mixture of waste bread and waste water from the production of wheat gluten was studied in this work. The lactic acid fermentation was performed with a probiotic lactic acid bacteria Lactobacillus rhamnosus ATCC 7469. During the fermentation, parameters such as the concentration of lactic acid (according to Taylor method), the concentration of reducing sugars (spectrophotometric method according to Miller), pH, and the bacterial growth (counting method on agar plates) were followed. The optimal conditions for the production of lactic acid such as: temperature, inoculum concentration, concentration of added CaCO3 for neutralization and shaking intensity were determined. Under these conditions, a yield of over 80% of the theoretical yield was achieved. The highest intensity of the lactic acid production was noticed during the first 24 hours of fermentation, while the fermentation completely ended after 72 hours. An intensive growth of the L. rhamnosus ATCC 7469 was detected under the optimal conditions, indicating a possibility of the parallel production of lactic acid and probiotic biomass. This approach could be economically more favorable. The liquid stillage used in this study was shown as a good substrate for lactic acid fermentation., U radu je ispitivana proizvodnja mlečne kiseline na tečnoj destilerijskoj džibri nastaloj nakon proizvodnje bioetanola iz smeše otpadnog hleba i otpadne vode iz proizvodnje glutena. Mlečno-kiselinska fermentacija je vršena pomoću probiotički aktivne bakterije Lactobacillus rhamnosus ATCC 7469. U toku fermentacije praćen je sadržaj mlečne kiseline (po metodi Taylor-a), sadržaj redukujućih šećera (spektrofotometrijski metodom po Miller-u), pH, kao i rast bakterije (brojanjem ćelija Koh-ovom metodom na agarnim pločama). Utvrđeni su optimalni uslovi za proizvodnju mlečne kiseline: temperatura, koncentracija inokuluma, koncentracija dodatog CaCO3 kao sredstva za neutralizaciju i intenzitet mešanja. Pri utvrđenim optimalnim uslovima ostvareni su prinosi od preko 80% od teorijskog prinosa mlečne kiseline u mikroaerofilnoj fermentaciji sa mešanjem. Najveći intezitet fermentacije ostvaren je u toku prvih 24 časa, a fermentacija je završena nakon 72 časa. Pri optimalnim uslovima mlečnokiselinske fermentacije uočen je intenzivan rast bakterije Lactobacillus rhamnosus ATCC 7469 što ukazuje na mogućnost paralelne proizvodnje mlečne kiseline i probiotičke biomase, čime bi se mogli postići bolji ekonomski efekti. Ispitivana džibra se pokazala kao pogodan medijum za mlečno kiselinsku fermentaciju.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "The production of lactic acid on liquid distillery stillage by Lactobacillus rhamnosus ATCC 7469, Proizvodnja mlečne kiseline na tečnoj destilerijskoj džibri pomoću Lactobacillus rhamnosus ATCC 7469",
pages = "104-96",
number = "20",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1817"
}

Đukić-Vuković, A., Mojović, L., Pejin, D., Vukašinović-Sekulić, M., Rakin, M., Nikolić, S.,& Pejin, J.. (2011). The production of lactic acid on liquid distillery stillage by Lactobacillus rhamnosus ATCC 7469. in Zbornik radova Tehnološkog fakulteta, Leskovac
Univerzitet u Nišu - Tehnološki fakultet, Leskovac.(20), 96-104.
https://hdl.handle.net/21.15107/rcub_technorep_1817

Đukić-Vuković A, Mojović L, Pejin D, Vukašinović-Sekulić M, Rakin M, Nikolić S, Pejin J. The production of lactic acid on liquid distillery stillage by Lactobacillus rhamnosus ATCC 7469. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2011;(20):96-104.
https://hdl.handle.net/21.15107/rcub_technorep_1817 .

Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Pejin, Dušanka, Vukašinović-Sekulić, Maja, Rakin, Marica, Nikolić, Svetlana, Pejin, Jelena, "The production of lactic acid on liquid distillery stillage by Lactobacillus rhamnosus ATCC 7469" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 20 (2011):96-104,
https://hdl.handle.net/21.15107/rcub_technorep_1817 .

TY  - JOUR
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Vukašinović-Sekulić, Maja
AU  - Rakin, Marica
AU  - Nikolić, Svetlana
AU  - Pejin, Jelena
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1786
AB  - Lactic acid is a relatively cheap chemical with a wide range of applications: as a preservative and acidifying agent in food and dairy industry, a monomer for biodegradable poly-lactide polymers (PLA) in pharmaceutical industry, precursor and chemical feedstock for chemical, textile and leather industries. Traditional raw materials for fermentative production of lactic acid, refined sugars, are now being replaced with starch from corn, rice and other crops for industrial production, with a tendency for utilization of agro-industrial wastes. Processes based on renewable waste sources have ecological (zero CO2 emission, eco-friendly byproducts) and economical (cheap raw materials, reduction of storage costs) advantages. An intensive research interest has been recently devoted to develop and improve the lactic acid production on more complex industrial by-products, like thin stillage from bioethanol production, corncobs, paper waste, straw etc. Complex and variable chemical composition and purity of these raw materials and high nutritional requirements of lacid acid bacteria (LAB) are the main obstacles in these production processes. Media supplementation to improve the fermentation is an important factor, especially from an economic point of view. Today, a particular challenge is to increase the productivity of lactic acid production on complex renewable biomass. Several strategies are currently being explored for this purpose such as process integration, use of LAB with amylolytic activity, employment of mixed cultures of LAB and/or utilization of genetically engineered microorganisms. Modern techniques of genetic engineering enable construction of microorganisms with desired characteristics and implementation of single step processes without or with minimal pre-treatment. In addition, new bioreactor constructions (such as membrane bioreactors), utilization of immobilized systems are also being explored. Electrodialysis, bipolar membrane separation process, enhanced filtration techniques etc., can provide some progress in purification technologies, although it is still remaining the most expensive phase in the lactic acid production. A new approach of parallel production of lactic bacteria biomass with probiotic activity and lactic acid could provide additional benefit and profit rise in the production process.
AB  - Potražnja za mlečnom kiselinom beleži snažan rast u poslednjoj deceniji, prvenstveno zbog razvoja biodegradabilnih polimera na bazi mlečne kiseline i njene primene u prehrambenoj, farmaceutskoj i drugim industrijama. Savremena proizvodnja mlečne kiseline se zasniva na principima održivosti, ekonomske i ekološke povoljnosti u biotehnološkim postupcima na obnovljivim i otpadnim poljoprivrednim sirovinama. U ovom radu su razmatrane mogućnosti fermentativne proizvodnje mlečne kiseline na različitim obnovljivim sirovinama, sa detaljnijom analizom mogućnosti korišćenja tečne džibre kao sirovine. Poseban izazov je povećati produktivnost proizvodnje na obnovljivim sirovinama korišćenjem novih tipova bioreaktora, bakterija mlečne kiseline (BMK) sa amilolitičkom aktivnošću, mešovitih kultura BMK ili pak genetički modifikovanih mikroorganizama. Primena novih tehnoloških rešenja koja uključuju integraciju određenih tehnoloških faza ili procesa, primenu imobilisanih sistema kao i uporednu proizvodnju mlečne kiseline i probiotske biomase je razmatrana u ovom radu.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - New trends and challenges in lactic acid production on renewable biomass
T1  - Novi pravci i izazovi u proizvodnji mlečne kiseline na obnovljivim sirovinama
EP  - 422
IS  - 4
SP  - 411
VL  - 65
DO  - 10.2298/HEMIND110114022D
ER  - 

@article{
author = "Đukić-Vuković, Aleksandra and Mojović, Ljiljana and Pejin, Dušanka and Vukašinović-Sekulić, Maja and Rakin, Marica and Nikolić, Svetlana and Pejin, Jelena",
year = "2011",
abstract = "Lactic acid is a relatively cheap chemical with a wide range of applications: as a preservative and acidifying agent in food and dairy industry, a monomer for biodegradable poly-lactide polymers (PLA) in pharmaceutical industry, precursor and chemical feedstock for chemical, textile and leather industries. Traditional raw materials for fermentative production of lactic acid, refined sugars, are now being replaced with starch from corn, rice and other crops for industrial production, with a tendency for utilization of agro-industrial wastes. Processes based on renewable waste sources have ecological (zero CO2 emission, eco-friendly byproducts) and economical (cheap raw materials, reduction of storage costs) advantages. An intensive research interest has been recently devoted to develop and improve the lactic acid production on more complex industrial by-products, like thin stillage from bioethanol production, corncobs, paper waste, straw etc. Complex and variable chemical composition and purity of these raw materials and high nutritional requirements of lacid acid bacteria (LAB) are the main obstacles in these production processes. Media supplementation to improve the fermentation is an important factor, especially from an economic point of view. Today, a particular challenge is to increase the productivity of lactic acid production on complex renewable biomass. Several strategies are currently being explored for this purpose such as process integration, use of LAB with amylolytic activity, employment of mixed cultures of LAB and/or utilization of genetically engineered microorganisms. Modern techniques of genetic engineering enable construction of microorganisms with desired characteristics and implementation of single step processes without or with minimal pre-treatment. In addition, new bioreactor constructions (such as membrane bioreactors), utilization of immobilized systems are also being explored. Electrodialysis, bipolar membrane separation process, enhanced filtration techniques etc., can provide some progress in purification technologies, although it is still remaining the most expensive phase in the lactic acid production. A new approach of parallel production of lactic bacteria biomass with probiotic activity and lactic acid could provide additional benefit and profit rise in the production process., Potražnja za mlečnom kiselinom beleži snažan rast u poslednjoj deceniji, prvenstveno zbog razvoja biodegradabilnih polimera na bazi mlečne kiseline i njene primene u prehrambenoj, farmaceutskoj i drugim industrijama. Savremena proizvodnja mlečne kiseline se zasniva na principima održivosti, ekonomske i ekološke povoljnosti u biotehnološkim postupcima na obnovljivim i otpadnim poljoprivrednim sirovinama. U ovom radu su razmatrane mogućnosti fermentativne proizvodnje mlečne kiseline na različitim obnovljivim sirovinama, sa detaljnijom analizom mogućnosti korišćenja tečne džibre kao sirovine. Poseban izazov je povećati produktivnost proizvodnje na obnovljivim sirovinama korišćenjem novih tipova bioreaktora, bakterija mlečne kiseline (BMK) sa amilolitičkom aktivnošću, mešovitih kultura BMK ili pak genetički modifikovanih mikroorganizama. Primena novih tehnoloških rešenja koja uključuju integraciju određenih tehnoloških faza ili procesa, primenu imobilisanih sistema kao i uporednu proizvodnju mlečne kiseline i probiotske biomase je razmatrana u ovom radu.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "New trends and challenges in lactic acid production on renewable biomass, Novi pravci i izazovi u proizvodnji mlečne kiseline na obnovljivim sirovinama",
pages = "422-411",
number = "4",
volume = "65",
doi = "10.2298/HEMIND110114022D"
}

Đukić-Vuković, A., Mojović, L., Pejin, D., Vukašinović-Sekulić, M., Rakin, M., Nikolić, S.,& Pejin, J.. (2011). New trends and challenges in lactic acid production on renewable biomass. in Hemijska industrija
Association of Chemical Engineers of Serbia., 65(4), 411-422.
https://doi.org/10.2298/HEMIND110114022D

Đukić-Vuković A, Mojović L, Pejin D, Vukašinović-Sekulić M, Rakin M, Nikolić S, Pejin J. New trends and challenges in lactic acid production on renewable biomass. in Hemijska industrija. 2011;65(4):411-422.
doi:10.2298/HEMIND110114022D .

Đukić-Vuković, Aleksandra, Mojović, Ljiljana, Pejin, Dušanka, Vukašinović-Sekulić, Maja, Rakin, Marica, Nikolić, Svetlana, Pejin, Jelena, "New trends and challenges in lactic acid production on renewable biomass" in Hemijska industrija, 65, no. 4 (2011):411-422,
https://doi.org/10.2298/HEMIND110114022D . .

TY  - CONF
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Pejin, Jelena
AU  - Vukašinović-Sekulić, Maja
AU  - Nikolić, S.
AU  - Markov, Siniša
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1751
AB  - The rapid depletion of the world fossil fuel supply and the increasing problem of greenhouse gas effects have strenghtened the worldwide interest in alternative sources of energy. Bioethanol accounts for the majority of biofuel use worldwide, either as a fuel or a gasoline enhancer. Utilization of bioethanol can significantly reduce fossil fuels use and exhaust greenhouse gas emission. The production of this fuel is increasing over the years, and has reached the level of 73.9 billion liters during the year 2009. In Serbia, the industrial production of bioethanol still relies on conventional energy crops containing starch and sugar such as corn, wheat and molasses. In order to improve the economy of bioethanol production and to avoid the competition of the feedstock utilization for food and energy, several production approaches based on crop selection, process integration and waste utilization were considered: (a) utilization of damaged crops not appropriate for food consumption; (b) utilization of triticale, the plant resistent to severe climate and soil conditions, which also possess its own source of amylolytic enzymes; (c) utilization of lignocellulosic biomass and technologies for the production of second generation ethanol; (d) implementation of thin stillage recycling step; (e) utilization of thin stillage for the production of lactic acid and/or microbial biomass with probiotic activity; (f) evaluation of the solid stillage remained after ethanol distillation as animal feed. The investigated approaches can significantly improve the economy of bioethanol production. In addition, some of the proposed processes utilize the wastes from bioethanol production and thus contribute to solve serious environmental problems.
PB  - 24th International Conference on Efficiency, Cost, Optimization, Simulation and Environmental Impact of Energy Systems, ECOS 2011
C3  - Proceedings of the 24th International Conference on Efficiency, Cost, Optimization, Simulation and E
T1  - The ways to improve the economy of bioethanol production in Serbia
EP  - 3614
SP  - 3603
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1751
ER  - 

@conference{
author = "Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Pejin, Jelena and Vukašinović-Sekulić, Maja and Nikolić, S. and Markov, Siniša",
year = "2011",
abstract = "The rapid depletion of the world fossil fuel supply and the increasing problem of greenhouse gas effects have strenghtened the worldwide interest in alternative sources of energy. Bioethanol accounts for the majority of biofuel use worldwide, either as a fuel or a gasoline enhancer. Utilization of bioethanol can significantly reduce fossil fuels use and exhaust greenhouse gas emission. The production of this fuel is increasing over the years, and has reached the level of 73.9 billion liters during the year 2009. In Serbia, the industrial production of bioethanol still relies on conventional energy crops containing starch and sugar such as corn, wheat and molasses. In order to improve the economy of bioethanol production and to avoid the competition of the feedstock utilization for food and energy, several production approaches based on crop selection, process integration and waste utilization were considered: (a) utilization of damaged crops not appropriate for food consumption; (b) utilization of triticale, the plant resistent to severe climate and soil conditions, which also possess its own source of amylolytic enzymes; (c) utilization of lignocellulosic biomass and technologies for the production of second generation ethanol; (d) implementation of thin stillage recycling step; (e) utilization of thin stillage for the production of lactic acid and/or microbial biomass with probiotic activity; (f) evaluation of the solid stillage remained after ethanol distillation as animal feed. The investigated approaches can significantly improve the economy of bioethanol production. In addition, some of the proposed processes utilize the wastes from bioethanol production and thus contribute to solve serious environmental problems.",
publisher = "24th International Conference on Efficiency, Cost, Optimization, Simulation and Environmental Impact of Energy Systems, ECOS 2011",
journal = "Proceedings of the 24th International Conference on Efficiency, Cost, Optimization, Simulation and E",
title = "The ways to improve the economy of bioethanol production in Serbia",
pages = "3614-3603",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1751"
}

Mojović, L., Pejin, D., Rakin, M., Pejin, J., Vukašinović-Sekulić, M., Nikolić, S.,& Markov, S.. (2011). The ways to improve the economy of bioethanol production in Serbia. in Proceedings of the 24th International Conference on Efficiency, Cost, Optimization, Simulation and E
24th International Conference on Efficiency, Cost, Optimization, Simulation and Environmental Impact of Energy Systems, ECOS 2011., 3603-3614.
https://hdl.handle.net/21.15107/rcub_technorep_1751

Mojović L, Pejin D, Rakin M, Pejin J, Vukašinović-Sekulić M, Nikolić S, Markov S. The ways to improve the economy of bioethanol production in Serbia. in Proceedings of the 24th International Conference on Efficiency, Cost, Optimization, Simulation and E. 2011;:3603-3614.
https://hdl.handle.net/21.15107/rcub_technorep_1751 .

Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Pejin, Jelena, Vukašinović-Sekulić, Maja, Nikolić, S., Markov, Siniša, "The ways to improve the economy of bioethanol production in Serbia" in Proceedings of the 24th International Conference on Efficiency, Cost, Optimization, Simulation and E (2011):3603-3614,
https://hdl.handle.net/21.15107/rcub_technorep_1751 .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Pejin, Jelena
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1944
AB  - Bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal by Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The optimal duration of the pretreatments and the SSF process kinetics were assessed and determined. Also, the effect of ultrasound and microwave pretreatments on ethanol yield and productivity was investigated. An optimal duration of 5 min was determined for both pretreatments. Ultrasonic and microwave pretreatments effectively increased the glucose concentration obtained after liquefaction by 6.82 and 8.48%, respectively, compared to untreated control sample. Also, both pretreatments improved ethanol yield and productivity during the SSF process. Ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process by 11.15 and 13.40% (compared to the control sample), respectively. The application of microwave pretreatment resulted in higher glucose release during liquefaction and consequently in higher ethanol concentration, compared to ultrasound pretreatment. A maximum ethanol concentration of 9.91% (w/w) and percentage of theoretical ethanol yield of 92.27% were achieved after 44 h of the SSF process of corn meal with prior microwave treatment.
PB  - Springer, New York
T2  - Clean Technologies and Environmental Policy
T1  - Utilization of microwave and ultrasound pretreatments in the production of bioethanol from corn
EP  - 594
IS  - 4
SP  - 587
VL  - 13
DO  - 10.1007/s10098-011-0366-0
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Pejin, Jelena",
year = "2011",
abstract = "Bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal by Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The optimal duration of the pretreatments and the SSF process kinetics were assessed and determined. Also, the effect of ultrasound and microwave pretreatments on ethanol yield and productivity was investigated. An optimal duration of 5 min was determined for both pretreatments. Ultrasonic and microwave pretreatments effectively increased the glucose concentration obtained after liquefaction by 6.82 and 8.48%, respectively, compared to untreated control sample. Also, both pretreatments improved ethanol yield and productivity during the SSF process. Ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process by 11.15 and 13.40% (compared to the control sample), respectively. The application of microwave pretreatment resulted in higher glucose release during liquefaction and consequently in higher ethanol concentration, compared to ultrasound pretreatment. A maximum ethanol concentration of 9.91% (w/w) and percentage of theoretical ethanol yield of 92.27% were achieved after 44 h of the SSF process of corn meal with prior microwave treatment.",
publisher = "Springer, New York",
journal = "Clean Technologies and Environmental Policy",
title = "Utilization of microwave and ultrasound pretreatments in the production of bioethanol from corn",
pages = "594-587",
number = "4",
volume = "13",
doi = "10.1007/s10098-011-0366-0"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Pejin, J.. (2011). Utilization of microwave and ultrasound pretreatments in the production of bioethanol from corn. in Clean Technologies and Environmental Policy
Springer, New York., 13(4), 587-594.
https://doi.org/10.1007/s10098-011-0366-0

Nikolić S, Mojović L, Rakin M, Pejin D, Pejin J. Utilization of microwave and ultrasound pretreatments in the production of bioethanol from corn. in Clean Technologies and Environmental Policy. 2011;13(4):587-594.
doi:10.1007/s10098-011-0366-0 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Pejin, Jelena, "Utilization of microwave and ultrasound pretreatments in the production of bioethanol from corn" in Clean Technologies and Environmental Policy, 13, no. 4 (2011):587-594,
https://doi.org/10.1007/s10098-011-0366-0 . .

TY  - JOUR
AU  - Marković, Milica
AU  - Markov, Siniša
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Jelena
AU  - Joković, Nataša
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1849
AB  - Triticale stillage is a by-product of bioethanol production. A research study was conducted in order to see if triticale stillage is adequate for lactic acid bacteria growth and lactic acid fermentation. Three Lactobacillus strains: Lactobacillus fermentum NRRL-B-75624, Lactobacillus fermentum PL-1, and Lactobacillus plantarum PL-4 were taken into consideration. Lactic acid fermentation was monitored by measuring pH value and titratable acidity. Lactobacillus fermentum PL-1 had the greatest decrease of pH values and increase of titratable acidity so it was chosen for future work. During the research, it was investigated how nutrient composition of triticale stillage and CaCO3 can influence lactic acid fermentation and CaCO3 role in cell protection. The nutrient composition of triticale stillage was satisfactory for lactic acid fermentation. The addition of CaCO3 helped in lactic acid fermentation. Although the titratable acidity in the samples with CaCO3 was lower than in the samples without CaCO3, the number of viable cells was higher for the samples with CaCO3, which showed that CaCO3 protected lactic acid cells from inhibition by lactic acid.
AB  - Džibra tritikalea predstavlja jedan od sporednih produkata tokom proizvodnje bioetanola. Glavna ideja istraživanja je bila da se istraži pogodnost džibre tritikalea kao podloge za uzgajanje bakterija mlečne kiseline i mlečnokiselinsku fermentaciju. Tokom rada su ispitivana tri soja bakterija mlečne kiseline: Lactobacillus fermentum NRRL-B-75624, Lactobacillus fermentum PL-1 i Lactobacillus plantarum PL-4. Tok mlečno-kiselinske fermentacije praćen je merenjem pH vrednosti uzorka, kao i određivanjem titrabilne kiselosti. Kod Lactobacillus fermentum PL-1 je zabeleženo najveće sniženje pH vrednosti, kao i porast titrabilne kiselosti, te je iz tih razloga izabran za dalji tok rada. Istraživanje je bilo usmereno i na to kako hranjivi sastav podloge i dodatak CaCO3 utiču na mlečno-kiselinsku fermentaciju, kao i na zaštitnu ulogu CaCO3 na ćelije bakterija mlečne kiseline. Hranjivi sastav džibre tritikalea se pokazao kao kompletna podloga za odvijanje mlečno-kiselinske fermentacije. Titrabilna kiselost je bila niža kod uzoraka sa dodatkom CaCO3, u odnosu na uzorke kojima nije dodat CaCO3, zbog formiranja Ca-laktata. Istovremeno je zabeležen veći broj vijabilnih ćelija u uzorcima kod kojih je dodat CaCO3, što ukazuje da CaCO3 ima zaštitnu ulogu na ćelije bakterija mlečne kiseline.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - The possibility of lactic acid fermentation in the Triticale stillage
T1  - Mogućnost mlečno-kiselinske fermentacije na džibri tritikalea
EP  - 162
IS  - 2
SP  - 153
VL  - 17
DO  - 10.2298/CICEQ100916065M
ER  - 

@article{
author = "Marković, Milica and Markov, Siniša and Pejin, Dušanka and Mojović, Ljiljana and Vukašinović-Sekulić, Maja and Pejin, Jelena and Joković, Nataša",
year = "2011",
abstract = "Triticale stillage is a by-product of bioethanol production. A research study was conducted in order to see if triticale stillage is adequate for lactic acid bacteria growth and lactic acid fermentation. Three Lactobacillus strains: Lactobacillus fermentum NRRL-B-75624, Lactobacillus fermentum PL-1, and Lactobacillus plantarum PL-4 were taken into consideration. Lactic acid fermentation was monitored by measuring pH value and titratable acidity. Lactobacillus fermentum PL-1 had the greatest decrease of pH values and increase of titratable acidity so it was chosen for future work. During the research, it was investigated how nutrient composition of triticale stillage and CaCO3 can influence lactic acid fermentation and CaCO3 role in cell protection. The nutrient composition of triticale stillage was satisfactory for lactic acid fermentation. The addition of CaCO3 helped in lactic acid fermentation. Although the titratable acidity in the samples with CaCO3 was lower than in the samples without CaCO3, the number of viable cells was higher for the samples with CaCO3, which showed that CaCO3 protected lactic acid cells from inhibition by lactic acid., Džibra tritikalea predstavlja jedan od sporednih produkata tokom proizvodnje bioetanola. Glavna ideja istraživanja je bila da se istraži pogodnost džibre tritikalea kao podloge za uzgajanje bakterija mlečne kiseline i mlečnokiselinsku fermentaciju. Tokom rada su ispitivana tri soja bakterija mlečne kiseline: Lactobacillus fermentum NRRL-B-75624, Lactobacillus fermentum PL-1 i Lactobacillus plantarum PL-4. Tok mlečno-kiselinske fermentacije praćen je merenjem pH vrednosti uzorka, kao i određivanjem titrabilne kiselosti. Kod Lactobacillus fermentum PL-1 je zabeleženo najveće sniženje pH vrednosti, kao i porast titrabilne kiselosti, te je iz tih razloga izabran za dalji tok rada. Istraživanje je bilo usmereno i na to kako hranjivi sastav podloge i dodatak CaCO3 utiču na mlečno-kiselinsku fermentaciju, kao i na zaštitnu ulogu CaCO3 na ćelije bakterija mlečne kiseline. Hranjivi sastav džibre tritikalea se pokazao kao kompletna podloga za odvijanje mlečno-kiselinske fermentacije. Titrabilna kiselost je bila niža kod uzoraka sa dodatkom CaCO3, u odnosu na uzorke kojima nije dodat CaCO3, zbog formiranja Ca-laktata. Istovremeno je zabeležen veći broj vijabilnih ćelija u uzorcima kod kojih je dodat CaCO3, što ukazuje da CaCO3 ima zaštitnu ulogu na ćelije bakterija mlečne kiseline.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "The possibility of lactic acid fermentation in the Triticale stillage, Mogućnost mlečno-kiselinske fermentacije na džibri tritikalea",
pages = "162-153",
number = "2",
volume = "17",
doi = "10.2298/CICEQ100916065M"
}

Marković, M., Markov, S., Pejin, D., Mojović, L., Vukašinović-Sekulić, M., Pejin, J.,& Joković, N.. (2011). The possibility of lactic acid fermentation in the Triticale stillage. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 17(2), 153-162.
https://doi.org/10.2298/CICEQ100916065M

Marković M, Markov S, Pejin D, Mojović L, Vukašinović-Sekulić M, Pejin J, Joković N. The possibility of lactic acid fermentation in the Triticale stillage. in Chemical Industry & Chemical Engineering Quarterly. 2011;17(2):153-162.
doi:10.2298/CICEQ100916065M .

Marković, Milica, Markov, Siniša, Pejin, Dušanka, Mojović, Ljiljana, Vukašinović-Sekulić, Maja, Pejin, Jelena, Joković, Nataša, "The possibility of lactic acid fermentation in the Triticale stillage" in Chemical Industry & Chemical Engineering Quarterly, 17, no. 2 (2011):153-162,
https://doi.org/10.2298/CICEQ100916065M . .

TY  - JOUR
AU  - Marković, Milica
AU  - Markov, Siniša
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Grujić, Olgica
AU  - Savatović, Slađana
AU  - Pejin, Jelena
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1818
AB  - Triticale shows many agronomic advantages including tolerance of acid soils, light soils, and dry conditions. This crop can be used for the ethanol production, and 8 triticale varieties were examined for it. The aim of the research was to choose a triticale variety with the lowest Falling number and the highest ethanol yield. In further research, the main aim was to see if the triticale stillage (produced during the ethanol production) can be used for the lactic acid production. According to technological parameters, the best variety is Odisej. Thermal preparation of triticale was made with distilled and tap water, but without noticeable differences in the ethanol yield. During lactic acid fermentation higher acidity was recorded in the samples without agitation, while the concentration of D- and L- lactic acid was higher in the samples with agitation. Utilization of carbohydrates was the same in both cases. The Odisej variety has the best technological characteristics, low Falling number, high α- amylase activity, no need for technical enzymes during the preparation process, as well as a high ethanol yield. Triticale stillage is a good medium for the lactic acid production, which can be used for natural preservation of stillage and its application in feed.
AB  - Tritikale ima mnogo agronomskih prednosti, i može rasti na kiselim i siromašnim zemljištima, kao i pri sušnim uslovima. Tritikale se može koristiti za proizvodnju bioetanola, i ispitano je 8 sorti ovog žita. Cilj rada je bio izabrati sortu tritikalea, sa najnižim brojem padanja i najvišim prinosom bioetanola. Dalji cilj istraživanja je bio da se ispita da li džibra dobijena nakon proizvodnje bioetanola može da se koristi za proizvodnju mlečne kiseline. Na osnovu tehnoloških parametara najbolja sorta je Odisej. Proces termičke pripreme tritikalea izvođen je sa destilovanom i vodom za piće, pri čemu nisu uočene razlike u prinosu bioetanola. Tokom mlečnokiselinske fermentacije uočena je nešto veća kiselost kod uzoraka bez mešanja, veće koncentracije D- i L- mlečne kiseline kod uzoraka sa mešanjem, dok je utrošak glukoze bio isti u oba slučaja. Sorta Odisej ima najbolje tehnološke karakteristike, nizak broj padanja, visoku α-amilaznu aktivnost, nisu neophodni tehnički enzimi za proces pripreme i daje visok prinos bioetanola. Džibra tritikalea je dobra podloga za proizvodnju mlečne kiseline što je moguće iskorititi za prirodno konzervisanje džibre i njenu primenu u stočnoj ishrani.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Triticale usage in the biotechnological processes: Bioethanol and lactic acid production
T1  - Primena tritikalea u biotehnološkim procesima - proizvodnja bioetanola i mlečne kiseline
EP  - 113
IS  - 20
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1818
ER  - 

@article{
author = "Marković, Milica and Markov, Siniša and Pejin, Dušanka and Mojović, Ljiljana and Grujić, Olgica and Savatović, Slađana and Pejin, Jelena",
year = "2011",
abstract = "Triticale shows many agronomic advantages including tolerance of acid soils, light soils, and dry conditions. This crop can be used for the ethanol production, and 8 triticale varieties were examined for it. The aim of the research was to choose a triticale variety with the lowest Falling number and the highest ethanol yield. In further research, the main aim was to see if the triticale stillage (produced during the ethanol production) can be used for the lactic acid production. According to technological parameters, the best variety is Odisej. Thermal preparation of triticale was made with distilled and tap water, but without noticeable differences in the ethanol yield. During lactic acid fermentation higher acidity was recorded in the samples without agitation, while the concentration of D- and L- lactic acid was higher in the samples with agitation. Utilization of carbohydrates was the same in both cases. The Odisej variety has the best technological characteristics, low Falling number, high α- amylase activity, no need for technical enzymes during the preparation process, as well as a high ethanol yield. Triticale stillage is a good medium for the lactic acid production, which can be used for natural preservation of stillage and its application in feed., Tritikale ima mnogo agronomskih prednosti, i može rasti na kiselim i siromašnim zemljištima, kao i pri sušnim uslovima. Tritikale se može koristiti za proizvodnju bioetanola, i ispitano je 8 sorti ovog žita. Cilj rada je bio izabrati sortu tritikalea, sa najnižim brojem padanja i najvišim prinosom bioetanola. Dalji cilj istraživanja je bio da se ispita da li džibra dobijena nakon proizvodnje bioetanola može da se koristi za proizvodnju mlečne kiseline. Na osnovu tehnoloških parametara najbolja sorta je Odisej. Proces termičke pripreme tritikalea izvođen je sa destilovanom i vodom za piće, pri čemu nisu uočene razlike u prinosu bioetanola. Tokom mlečnokiselinske fermentacije uočena je nešto veća kiselost kod uzoraka bez mešanja, veće koncentracije D- i L- mlečne kiseline kod uzoraka sa mešanjem, dok je utrošak glukoze bio isti u oba slučaja. Sorta Odisej ima najbolje tehnološke karakteristike, nizak broj padanja, visoku α-amilaznu aktivnost, nisu neophodni tehnički enzimi za proces pripreme i daje visok prinos bioetanola. Džibra tritikalea je dobra podloga za proizvodnju mlečne kiseline što je moguće iskorititi za prirodno konzervisanje džibre i njenu primenu u stočnoj ishrani.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Triticale usage in the biotechnological processes: Bioethanol and lactic acid production, Primena tritikalea u biotehnološkim procesima - proizvodnja bioetanola i mlečne kiseline",
pages = "113-105",
number = "20",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1818"
}

Marković, M., Markov, S., Pejin, D., Mojović, L., Grujić, O., Savatović, S.,& Pejin, J.. (2011). Triticale usage in the biotechnological processes: Bioethanol and lactic acid production. in Zbornik radova Tehnološkog fakulteta, Leskovac
Univerzitet u Nišu - Tehnološki fakultet, Leskovac.(20), 105-113.
https://hdl.handle.net/21.15107/rcub_technorep_1818

Marković M, Markov S, Pejin D, Mojović L, Grujić O, Savatović S, Pejin J. Triticale usage in the biotechnological processes: Bioethanol and lactic acid production. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2011;(20):105-113.
https://hdl.handle.net/21.15107/rcub_technorep_1818 .

Marković, Milica, Markov, Siniša, Pejin, Dušanka, Mojović, Ljiljana, Grujić, Olgica, Savatović, Slađana, Pejin, Jelena, "Triticale usage in the biotechnological processes: Bioethanol and lactic acid production" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 20 (2011):105-113,
https://hdl.handle.net/21.15107/rcub_technorep_1818 .

TY  - CONF
AU  - Marković, Milica
AU  - Markov, Siniša
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Vukašinović, Maja
AU  - Pejin, Jelena
AU  - Joković, Nataša
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6303
AB  - Triticale is a very applicable crop for bioethanol production, and also provides a stillage that can be used as a feed. The main aim of this research was to investigate the possibility of transformation of unused stillage carbohydrate after bioethanol prooduction into the lactis acid fermentation, in order to produce a natural feed preservative. In this study Lactobacillus fermentum Pl-1 was used. Different pre-treatment were done during the preparation of bioethanol fermentation (by triticale autoamylolytic enzymes, technical enzymes and ultrasound), and thus 4 different samples of stillage were prepared. CaCO3 was added in one part of the samples. Results indicated a good conversion factor of glucose into the lactic acid, up to 1.55 after 72 hours of the treatment. The sample pre-treated only by triticale autoamylolytic enzymes had a small part of carbohydrtes utilised during the stillage preparation. As a result, there was more glucose available for lactic acid fermentation. Added CaCo3 probably improved the protection of Lactobacillus fermentum PL-1 cells, but it was not possible to see a real glucose conversion factor due to Ca-lactate formation. Therefore, the stillage produced after the bioethanol production is a very good medium for lactic acid production
PB  - Beograd : Tehnološko-metalurški fakultet Univerziteta
C3  - Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine
T1  - Primena džibre tritikalea za proizvodnju mlečne kiseline
T1  - Triticale stillage appliance for lactic acid production
EP  - 33
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6303
ER  - 

@conference{
author = "Marković, Milica and Markov, Siniša and Pejin, Dušanka and Mojović, Ljiljana and Vukašinović, Maja and Pejin, Jelena and Joković, Nataša",
year = "2010",
abstract = "Triticale is a very applicable crop for bioethanol production, and also provides a stillage that can be used as a feed. The main aim of this research was to investigate the possibility of transformation of unused stillage carbohydrate after bioethanol prooduction into the lactis acid fermentation, in order to produce a natural feed preservative. In this study Lactobacillus fermentum Pl-1 was used. Different pre-treatment were done during the preparation of bioethanol fermentation (by triticale autoamylolytic enzymes, technical enzymes and ultrasound), and thus 4 different samples of stillage were prepared. CaCO3 was added in one part of the samples. Results indicated a good conversion factor of glucose into the lactic acid, up to 1.55 after 72 hours of the treatment. The sample pre-treated only by triticale autoamylolytic enzymes had a small part of carbohydrtes utilised during the stillage preparation. As a result, there was more glucose available for lactic acid fermentation. Added CaCo3 probably improved the protection of Lactobacillus fermentum PL-1 cells, but it was not possible to see a real glucose conversion factor due to Ca-lactate formation. Therefore, the stillage produced after the bioethanol production is a very good medium for lactic acid production",
publisher = "Beograd : Tehnološko-metalurški fakultet Univerziteta",
journal = "Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine",
title = "Primena džibre tritikalea za proizvodnju mlečne kiseline, Triticale stillage appliance for lactic acid production",
pages = "33-32",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6303"
}

Marković, M., Markov, S., Pejin, D., Mojović, L., Vukašinović, M., Pejin, J.,& Joković, N.. (2010). Primena džibre tritikalea za proizvodnju mlečne kiseline. in Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine
Beograd : Tehnološko-metalurški fakultet Univerziteta., 32-33.
https://hdl.handle.net/21.15107/rcub_technorep_6303

Marković M, Markov S, Pejin D, Mojović L, Vukašinović M, Pejin J, Joković N. Primena džibre tritikalea za proizvodnju mlečne kiseline. in Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine. 2010;:32-33.
https://hdl.handle.net/21.15107/rcub_technorep_6303 .

Marković, Milica, Markov, Siniša, Pejin, Dušanka, Mojović, Ljiljana, Vukašinović, Maja, Pejin, Jelena, Joković, Nataša, "Primena džibre tritikalea za proizvodnju mlečne kiseline" in Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine (2010):32-33,
https://hdl.handle.net/21.15107/rcub_technorep_6303 .

TY  - CONF
AU  - Nikolić, Svetlana
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Jelena
AU  - Djukić, Aleksandra
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6304
AB  - The lactic acid production from corn thin stillage using Lactobacillus rhamnosus ATCC 7469 in a batch system was studied. The fermentation temperature was optimized taking into account lactic acid production and glucose consumption as well as other significant process parameters (such as lactic acid yield and volumetric productivity). Also, the effect of pH control on lactic acid production by addition of 1% (w/w) CaCO3 and 10M NaOH was investigated. Based on the obtained results, an optimal fermentation temperature of 41 0C was selected. In this case, lactic acid yield of 0.70 g/g and utilized glucose of 72.55% were achieved. The addition of 10 M NaOH after 72h of fermentation at optimal temperature improved substrate (glucose) utilization for 63.70% compared to the control sample.
PB  - Технолошко-металуршки факултет, Београд
C3  - Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem
T1  - Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469
EP  - 51
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6304
ER  - 

@conference{
author = "Nikolić, Svetlana and Vukašinović-Sekulić, Maja and Pejin, Dušanka and Mojović, Ljiljana and Rakin, Marica and Pejin, Jelena and Djukić, Aleksandra",
year = "2010",
abstract = "The lactic acid production from corn thin stillage using Lactobacillus rhamnosus ATCC 7469 in a batch system was studied. The fermentation temperature was optimized taking into account lactic acid production and glucose consumption as well as other significant process parameters (such as lactic acid yield and volumetric productivity). Also, the effect of pH control on lactic acid production by addition of 1% (w/w) CaCO3 and 10M NaOH was investigated. Based on the obtained results, an optimal fermentation temperature of 41 0C was selected. In this case, lactic acid yield of 0.70 g/g and utilized glucose of 72.55% were achieved. The addition of 10 M NaOH after 72h of fermentation at optimal temperature improved substrate (glucose) utilization for 63.70% compared to the control sample.",
publisher = "Технолошко-металуршки факултет, Београд",
journal = "Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem",
title = "Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469",
pages = "51-50",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6304"
}

Nikolić, S., Vukašinović-Sekulić, M., Pejin, D., Mojović, L., Rakin, M., Pejin, J.,& Djukić, A.. (2010). Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469. in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem
Технолошко-металуршки факултет, Београд., 50-51.
https://hdl.handle.net/21.15107/rcub_technorep_6304

Nikolić S, Vukašinović-Sekulić M, Pejin D, Mojović L, Rakin M, Pejin J, Djukić A. Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469. in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem. 2010;:50-51.
https://hdl.handle.net/21.15107/rcub_technorep_6304 .

Nikolić, Svetlana, Vukašinović-Sekulić, Maja, Pejin, Dušanka, Mojović, Ljiljana, Rakin, Marica, Pejin, Jelena, Djukić, Aleksandra, "Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469" in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem (2010):50-51,
https://hdl.handle.net/21.15107/rcub_technorep_6304 .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1636
AB  - The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield and volumetric productivity. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v v(-1)) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. Optimal initial glucose concentrations of 150 and 176 g l(-1) for free and immobilized system were achieved, respectively. The immobilized cell system was superior to the free cell system since higher ethanol tolerance and productivity and lower substrate inhibition were detected.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Biomass & Bioenergy
T1  - Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 1456
IS  - 10
SP  - 1449
VL  - 34
DO  - 10.1016/j.biombioe.2010.04.008
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Vukašinović-Sekulić, Maja",
year = "2010",
abstract = "The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield and volumetric productivity. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v v(-1)) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. Optimal initial glucose concentrations of 150 and 176 g l(-1) for free and immobilized system were achieved, respectively. The immobilized cell system was superior to the free cell system since higher ethanol tolerance and productivity and lower substrate inhibition were detected.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Biomass & Bioenergy",
title = "Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "1456-1449",
number = "10",
volume = "34",
doi = "10.1016/j.biombioe.2010.04.008"
}

Nikolić, S., Mojović, L., Pejin, D., Rakin, M.,& Vukašinović-Sekulić, M.. (2010). Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Biomass & Bioenergy
Pergamon-Elsevier Science Ltd, Oxford., 34(10), 1449-1456.
https://doi.org/10.1016/j.biombioe.2010.04.008

Nikolić S, Mojović L, Pejin D, Rakin M, Vukašinović-Sekulić M. Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Biomass & Bioenergy. 2010;34(10):1449-1456.
doi:10.1016/j.biombioe.2010.04.008 .

Nikolić, Svetlana, Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Vukašinović-Sekulić, Maja, "Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Biomass & Bioenergy, 34, no. 10 (2010):1449-1456,
https://doi.org/10.1016/j.biombioe.2010.04.008 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Dušanka
AU  - Pejin, Jelena
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1738
AB  - Bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal by Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The SSF process kinetics were assessed and determined. Based on the obtained results, both pretreatments improved ethanol production during the SSF process. The ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process for 11.15 % and 13.40 % (compared to the control sample), respectively. The application of microwave pretreatment resulted in higher increase in ethanol concentration compared to the ultrasound pretreatment.
PB  - Aidic Servizi Srl, Milano
T2  - Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
T1  - Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments
EP  - 1332
SP  - 1327
VL  - 21
DO  - 10.3303/CET1021222
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Vukašinović-Sekulić, Maja and Pejin, Dušanka and Pejin, Jelena",
year = "2010",
abstract = "Bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal by Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The SSF process kinetics were assessed and determined. Based on the obtained results, both pretreatments improved ethanol production during the SSF process. The ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process for 11.15 % and 13.40 % (compared to the control sample), respectively. The application of microwave pretreatment resulted in higher increase in ethanol concentration compared to the ultrasound pretreatment.",
publisher = "Aidic Servizi Srl, Milano",
journal = "Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener",
title = "Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments",
pages = "1332-1327",
volume = "21",
doi = "10.3303/CET1021222"
}

Nikolić, S., Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Pejin, D.,& Pejin, J.. (2010). Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
Aidic Servizi Srl, Milano., 21, 1327-1332.
https://doi.org/10.3303/CET1021222

Nikolić S, Mojović L, Rakin M, Vukašinović-Sekulić M, Pejin D, Pejin J. Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener. 2010;21:1327-1332.
doi:10.3303/CET1021222 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Vukašinović-Sekulić, Maja, Pejin, Dušanka, Pejin, Jelena, "Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments" in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener, 21 (2010):1327-1332,
https://doi.org/10.3303/CET1021222 . .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Nikolić, Svetlana
AU  - Pejin, Jelena
AU  - Pejin, Dušanka
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1736
AB  - The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieved after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals.
PB  - Aidic Servizi Srl, Milano
T2  - Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
T1  - Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast
EP  - 1338
SP  - 1333
VL  - 21
DO  - 10.3303/CET1021223
ER  - 

@article{
author = "Mojović, Ljiljana and Rakin, Marica and Vukašinović-Sekulić, Maja and Nikolić, Svetlana and Pejin, Jelena and Pejin, Dušanka",
year = "2010",
abstract = "The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieved after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals.",
publisher = "Aidic Servizi Srl, Milano",
journal = "Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener",
title = "Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast",
pages = "1338-1333",
volume = "21",
doi = "10.3303/CET1021223"
}

Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Nikolić, S., Pejin, J.,& Pejin, D.. (2010). Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
Aidic Servizi Srl, Milano., 21, 1333-1338.
https://doi.org/10.3303/CET1021223

Mojović L, Rakin M, Vukašinović-Sekulić M, Nikolić S, Pejin J, Pejin D. Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener. 2010;21:1333-1338.
doi:10.3303/CET1021223 .

Mojović, Ljiljana, Rakin, Marica, Vukašinović-Sekulić, Maja, Nikolić, Svetlana, Pejin, Jelena, Pejin, Dušanka, "Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast" in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener, 21 (2010):1333-1338,
https://doi.org/10.3303/CET1021223 . .

TY  - CONF
AU  - Nikolić, S.
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Dušanka
AU  - Pejin, Jelena
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1547
AB  - Bioethanol production by simultaneous saccharification and fermentation (SSF) of cornmeal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The SSF process kinetics was determined. Both pretreatments improved ethanol production during the SSF process. The ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process for 11.15 and 13.40%, respectively. The maximum ethanol concentration of 9.87% (wt/wt) and the percentage of theoretical ethanol yield of 90.80% were achieved after 32 hr of the SSF of corn meal with prior microwave treatment. The application of microwave pretreatment resulted in higher increase in ethanol concentration compared to the ultrasound pretreatment. This is an abstract of a paper presented at the 19th International Congress of Chemical and Process Engineering and 7th European Congress of Chemical Engineering (Prague, Czech Republic 8/28/2010-9/1/2010).
PB  - 19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congress of Chemical Engineering, ECCE-7
C3  - 19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congres
T1  - Improvement of bioethanol production from corn by ultrasound and microwave pretreatments
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1547
ER  - 

@conference{
author = "Nikolić, S. and Mojović, Ljiljana and Rakin, Marica and Vukašinović-Sekulić, Maja and Pejin, Dušanka and Pejin, Jelena",
year = "2010",
abstract = "Bioethanol production by simultaneous saccharification and fermentation (SSF) of cornmeal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The SSF process kinetics was determined. Both pretreatments improved ethanol production during the SSF process. The ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process for 11.15 and 13.40%, respectively. The maximum ethanol concentration of 9.87% (wt/wt) and the percentage of theoretical ethanol yield of 90.80% were achieved after 32 hr of the SSF of corn meal with prior microwave treatment. The application of microwave pretreatment resulted in higher increase in ethanol concentration compared to the ultrasound pretreatment. This is an abstract of a paper presented at the 19th International Congress of Chemical and Process Engineering and 7th European Congress of Chemical Engineering (Prague, Czech Republic 8/28/2010-9/1/2010).",
publisher = "19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congress of Chemical Engineering, ECCE-7",
journal = "19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congres",
title = "Improvement of bioethanol production from corn by ultrasound and microwave pretreatments",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1547"
}

Nikolić, S., Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Pejin, D.,& Pejin, J.. (2010). Improvement of bioethanol production from corn by ultrasound and microwave pretreatments. in 19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congres
19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congress of Chemical Engineering, ECCE-7..
https://hdl.handle.net/21.15107/rcub_technorep_1547

Nikolić S, Mojović L, Rakin M, Vukašinović-Sekulić M, Pejin D, Pejin J. Improvement of bioethanol production from corn by ultrasound and microwave pretreatments. in 19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congres. 2010;.
https://hdl.handle.net/21.15107/rcub_technorep_1547 .

Nikolić, S., Mojović, Ljiljana, Rakin, Marica, Vukašinović-Sekulić, Maja, Pejin, Dušanka, Pejin, Jelena, "Improvement of bioethanol production from corn by ultrasound and microwave pretreatments" in 19th International Congress of Chemical and Process Engineering, CHISA 2010 and 7th European Congres (2010),
https://hdl.handle.net/21.15107/rcub_technorep_1547 .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Pejin, Jelena
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1698
AB  - An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 degrees C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of corn meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal
EP  - 222
IS  - 1
SP  - 216
VL  - 122
DO  - 10.1016/j.foodchem.2010.02.063
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Pejin, Jelena",
year = "2010",
abstract = "An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 degrees C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of corn meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal",
pages = "222-216",
number = "1",
volume = "122",
doi = "10.1016/j.foodchem.2010.02.063"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Pejin, J.. (2010). Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal. in Food Chemistry
Elsevier Sci Ltd, Oxford., 122(1), 216-222.
https://doi.org/10.1016/j.foodchem.2010.02.063

Nikolić S, Mojović L, Rakin M, Pejin D, Pejin J. Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal. in Food Chemistry. 2010;122(1):216-222.
doi:10.1016/j.foodchem.2010.02.063 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Pejin, Jelena, "Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal" in Food Chemistry, 122, no. 1 (2010):216-222,
https://doi.org/10.1016/j.foodchem.2010.02.063 . .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Jelena
AU  - Grujić, Olgica
AU  - Nikolić, Svetlana
AU  - Radosavljević, Milica
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1590
AB  - Bioethanol produced by fermentation of biomass, such as sugar, starch, or lignocellulosic materials, is one of the most important renewable energy resources. An average thin stillage amount produced in the bioethanol process is approximately 13 hL per hL of bioethanol. An adequate utilization of the stillage is of great importance in order to improve the economy of the bioethanol production. The aim of this paper was to study the possibilities of stillage utilization from the process of bioethanol production from corn. For this purpose the recirculation of thin stillage in the amount of 10-30% in mashing process was studied, while the solid phase remained after filtration was assessed for animal feed. The results have shown that the ethanol yield in the process can be significantly increased by the recirculation. The dry matter content in the slurry after the fermentation also increased with the increasing amount of recirculated stillage. Chemical composition of solid stillage remained after fermentation as well as of the solid stillage enriched with yeast (1 %of dry Saccharomyces cerevisiae yeast) was studied. The stillage enriched with yeast had a greater amount of proteins and generally can be used as a high quality feed.
AB  - Bioetanol proizveden postupkom fermentacije biomase na bazi šećera, skroba ili lignoceluloze predstavlja jedan od najznačajnijih obnovljivih izvora energije. Prilikom proizvodnje 1 hL etanola nastaje oko 13 hl bistre džibre. Da bi se povećala ekonomičnost ovog postupka od izuzetne važnosti je da se džibra koja nastaje kao otpadni proizvod adekvatno valorizuje. Cilj ovog rada je da se ispitaju mogućnosti iskorišćavanja džibre nastale prilikom proizvodnje bioetanola na kukuruzu. U radu je ispitivana recirkulacije bistre džibre u količini od 10-30% u fazu ukomljavanja kukuruza za proizvodnju etanola, dok je zaostala čvrsta faza nakon filtracije razmatrana za korišćenje u ishrani stoke. Utvrđeno je da se recirkulacijom može značajno povećati koncentracija etanola u proizvodnom procesu. Sadržaj suve materije u džibri zaostaloj nakon fermentacije se takodje povećavao sa povećanjem količine recirkulisane bistre džibre. U radu je ispitan hemijski sastav čvrste džibre i džibre obogaćene kvascem (1 % suvog Saccharomyces cerevisiae kvasca) i analizirana nutritivna vrednost ovih uzoraka. Džibra obogaćena kvascem je imala značajno veći sadržaj proteina i generalno se može koristiti kao visoko kvalitetna hrana za životinje.
PB  - Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
T2  - Journal on Processing and Energy in Agriculture
T1  - Investigations of the possibilities of stillage utilization from the bioethanol production on corn
T1  - Ispitivanje mogućnosti korišćenja džibre iz proizvodnje bioetanola iz kukuruza
EP  - 57
IS  - 1
SP  - 54
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1590
ER  - 

@article{
author = "Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Vukašinović-Sekulić, Maja and Pejin, Jelena and Grujić, Olgica and Nikolić, Svetlana and Radosavljević, Milica",
year = "2010",
abstract = "Bioethanol produced by fermentation of biomass, such as sugar, starch, or lignocellulosic materials, is one of the most important renewable energy resources. An average thin stillage amount produced in the bioethanol process is approximately 13 hL per hL of bioethanol. An adequate utilization of the stillage is of great importance in order to improve the economy of the bioethanol production. The aim of this paper was to study the possibilities of stillage utilization from the process of bioethanol production from corn. For this purpose the recirculation of thin stillage in the amount of 10-30% in mashing process was studied, while the solid phase remained after filtration was assessed for animal feed. The results have shown that the ethanol yield in the process can be significantly increased by the recirculation. The dry matter content in the slurry after the fermentation also increased with the increasing amount of recirculated stillage. Chemical composition of solid stillage remained after fermentation as well as of the solid stillage enriched with yeast (1 %of dry Saccharomyces cerevisiae yeast) was studied. The stillage enriched with yeast had a greater amount of proteins and generally can be used as a high quality feed., Bioetanol proizveden postupkom fermentacije biomase na bazi šećera, skroba ili lignoceluloze predstavlja jedan od najznačajnijih obnovljivih izvora energije. Prilikom proizvodnje 1 hL etanola nastaje oko 13 hl bistre džibre. Da bi se povećala ekonomičnost ovog postupka od izuzetne važnosti je da se džibra koja nastaje kao otpadni proizvod adekvatno valorizuje. Cilj ovog rada je da se ispitaju mogućnosti iskorišćavanja džibre nastale prilikom proizvodnje bioetanola na kukuruzu. U radu je ispitivana recirkulacije bistre džibre u količini od 10-30% u fazu ukomljavanja kukuruza za proizvodnju etanola, dok je zaostala čvrsta faza nakon filtracije razmatrana za korišćenje u ishrani stoke. Utvrđeno je da se recirkulacijom može značajno povećati koncentracija etanola u proizvodnom procesu. Sadržaj suve materije u džibri zaostaloj nakon fermentacije se takodje povećavao sa povećanjem količine recirkulisane bistre džibre. U radu je ispitan hemijski sastav čvrste džibre i džibre obogaćene kvascem (1 % suvog Saccharomyces cerevisiae kvasca) i analizirana nutritivna vrednost ovih uzoraka. Džibra obogaćena kvascem je imala značajno veći sadržaj proteina i generalno se može koristiti kao visoko kvalitetna hrana za životinje.",
publisher = "Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "Journal on Processing and Energy in Agriculture",
title = "Investigations of the possibilities of stillage utilization from the bioethanol production on corn, Ispitivanje mogućnosti korišćenja džibre iz proizvodnje bioetanola iz kukuruza",
pages = "57-54",
number = "1",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1590"
}

Mojović, L., Pejin, D., Rakin, M., Vukašinović-Sekulić, M., Pejin, J., Grujić, O., Nikolić, S.,& Radosavljević, M.. (2010). Investigations of the possibilities of stillage utilization from the bioethanol production on corn. in Journal on Processing and Energy in Agriculture
Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 14(1), 54-57.
https://hdl.handle.net/21.15107/rcub_technorep_1590

Mojović L, Pejin D, Rakin M, Vukašinović-Sekulić M, Pejin J, Grujić O, Nikolić S, Radosavljević M. Investigations of the possibilities of stillage utilization from the bioethanol production on corn. in Journal on Processing and Energy in Agriculture. 2010;14(1):54-57.
https://hdl.handle.net/21.15107/rcub_technorep_1590 .

Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Vukašinović-Sekulić, Maja, Pejin, Jelena, Grujić, Olgica, Nikolić, Svetlana, Radosavljević, Milica, "Investigations of the possibilities of stillage utilization from the bioethanol production on corn" in Journal on Processing and Energy in Agriculture, 14, no. 1 (2010):54-57,
https://hdl.handle.net/21.15107/rcub_technorep_1590 .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1500
AB  - The simultaneous enzymatic saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 center dot 7H(2)O and MgSO4 center dot 7H(2)O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/l.h were obtained.
PB  - Elsevier Sci Ltd, Oxford
T2  - Fuel
T1  - Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 1607
IS  - 9
SP  - 1602
VL  - 88
DO  - 10.1016/j.fuel.2008.12.019
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka",
year = "2009",
abstract = "The simultaneous enzymatic saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 center dot 7H(2)O and MgSO4 center dot 7H(2)O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/l.h were obtained.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fuel",
title = "Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "1607-1602",
number = "9",
volume = "88",
doi = "10.1016/j.fuel.2008.12.019"
}

Nikolić, S., Mojović, L., Rakin, M.,& Pejin, D.. (2009). Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Fuel
Elsevier Sci Ltd, Oxford., 88(9), 1602-1607.
https://doi.org/10.1016/j.fuel.2008.12.019

Nikolić S, Mojović L, Rakin M, Pejin D. Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Fuel. 2009;88(9):1602-1607.
doi:10.1016/j.fuel.2008.12.019 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, "Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Fuel, 88, no. 9 (2009):1602-1607,
https://doi.org/10.1016/j.fuel.2008.12.019 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Nedović, Viktor
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1485
AB  - BACKGROUND: Bioethanol produced from renewable biomass, such as corn meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca-alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L-1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca-alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca-alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 503
IS  - 4
SP  - 497
VL  - 84
DO  - 10.1002/jctb.2068
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Nedović, Viktor",
year = "2009",
abstract = "BACKGROUND: Bioethanol produced from renewable biomass, such as corn meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca-alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L-1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca-alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca-alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "503-497",
number = "4",
volume = "84",
doi = "10.1002/jctb.2068"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Nedović, V.. (2009). Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 84(4), 497-503.
https://doi.org/10.1002/jctb.2068

Nikolić S, Mojović L, Rakin M, Pejin D, Nedović V. Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Journal of Chemical Technology and Biotechnology. 2009;84(4):497-503.
doi:10.1002/jctb.2068 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Nedović, Viktor, "Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Journal of Chemical Technology and Biotechnology, 84, no. 4 (2009):497-503,
https://doi.org/10.1002/jctb.2068 . .