@conference{
author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Jaćimovski, Darko and Bezbradica, Dejan",
year = "2023",
abstract = "Scientific breakthroughs in research of human microbiota revealed that significant number of
diseases related to modern food intake and sedentary lifestyles are caused by dysbiosis of human
microbiota composition, hence substantial increase in interest for products helping restore this balance
(such as probiotics and prebiotics) within food, cosmetic and health industry was observed. In recent
times, prebiotics have been recognized as attractive products due to their higher stability to processing
and storage conditions, lower susceptibility to interaction with other compounds in more complex
formulation and higher reproducibility of their physiological effects [1]. Consequential rapid growth of
market demand brought challenges for biochemical industry, such as requirements to increase
production capacity and decrease costs by development of more efficient enzymatic processes and
product separation.
Majority of prebiotic compounds are classified as oligosaccharides, which can be obtained
either by enzymatic synthesis from compounds of lower degree of polymerization (DP) - for example:
galacto-oligosaccharides (GOS) from lactose and fructo-oligosaccharides (FOS) from sucrose - or by
enzymatic hydrolysis, for example pectic oligosaccharides (POS) from pectin or xylo-oligosaccharides
(XOS) from xylan [2]. In both cases, membrane separation is efficient tool for product purification,
because OS obtained by synthesis can be separated from lower DP compounds using nanofiltrations,
while those obtained by hydrolysis can be separated from compounds of higher DP using ultrafiltration.
Hence, it can be expected that selection of adequate membrane material, pore dimensions and
downstream processing conditions will have important role in development of novel prebiotic
technologies.
In this study nanofiltration membrane module was applied with a purpose of purification of
FOS obtained in enzymatic synthesis using sucrose as substrate. Two strategies were applied:
• purification of reaction mixture obtained after enzymatic synthesis,
• simultaneous enzymatic synthesis and purification in nanofiltration membrane module.",
publisher = "TECNALIA",
journal = "Proceedings of the 16th International Conference on Catalyst in Membrane Reactors (ICCMR16), October 16-18, 2023, Donostia-San Sebastián, Spain",
title = "Simultaneous enzymatic synthesis and purification of prebiotic fructooligosaccharides in nanofiltration membrane module",
pages = "85--84",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6871"
}