TY  - JOUR
AU  - Milivojević, Milan
AU  - Popović, Aleksandra
AU  - Pajić-Lijaković, Ivana
AU  - Šoštarić, Ivan
AU  - Kolašinac, Stefan
AU  - Dajić Stevanović, Zora
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6616
AB  - Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.
PB  - MDPI
T2  - Gels
T1  - Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications
IS  - 8
SP  - 620
VL  - 9
DO  - 10.3390/gels9080620
ER  - 

@article{
author = "Milivojević, Milan and Popović, Aleksandra and Pajić-Lijaković, Ivana and Šoštarić, Ivan and Kolašinac, Stefan and Dajić Stevanović, Zora",
year = "2023",
abstract = "Sodium alginate is one of the most interesting and the most investigated and applied biopolymers due to its advantageous properties. Among them, easy, simple, mild, rapid, non-toxic gelation by divalent cations is the most important. In addition, it is abundant, low-cost, eco-friendly, bio-compatible, bio-adhesive, biodegradable, stable, etc. All those properties were systematically considered within this review. Carotenoids are functional components in the human diet with plenty of health benefits. However, their sensitivity to environmental and process stresses, chemical instability, easy oxidation, low water solubility, and bioavailability limit their food and pharmaceutical applications. Encapsulation may help in overcoming these limitations and within this review, the role of alginate-based encapsulation systems in improving the stability and bioavailability of carotenoids is explored. It may be concluded that all alginate-based systems increase carotenoid stability, but only those of micro- and nano-size, as well as emulsion-based, may improve their low bioaccessibility. In addition, the incorporation of other biopolymers may further improve encapsulation system properties. Furthermore, the main techniques for evaluating the encapsulation are briefly considered. This review critically and profoundly explains the role of alginates in improving the encapsulation process of carotenoids, suggesting the best alternatives for those systems. Moreover, it provides a comprehensive cover of recent advances in this field.",
publisher = "MDPI",
journal = "Gels",
title = "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications",
number = "8",
pages = "620",
volume = "9",
doi = "10.3390/gels9080620"
}

Milivojević, M., Popović, A., Pajić-Lijaković, I., Šoštarić, I., Kolašinac, S.,& Dajić Stevanović, Z.. (2023). Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels
MDPI., 9(8), 620.
https://doi.org/10.3390/gels9080620

Milivojević M, Popović A, Pajić-Lijaković I, Šoštarić I, Kolašinac S, Dajić Stevanović Z. Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications. in Gels. 2023;9(8):620.
doi:10.3390/gels9080620 .

Milivojević, Milan, Popović, Aleksandra, Pajić-Lijaković, Ivana, Šoštarić, Ivan, Kolašinac, Stefan, Dajić Stevanović, Zora, "Alginate Gel-Based Carriers for Encapsulation of Carotenoids: On Challenges and Applications" in Gels, 9, no. 8 (2023):620,
https://doi.org/10.3390/gels9080620 . .

TY  - JOUR
AU  - Lučić, Milica
AU  - Potkonjak, Nebojša
AU  - Sredović Ignjatović, Ivana
AU  - Lević, Steva
AU  - Dajić-Stevanović, Zora
AU  - Kolašinac, Stefan
AU  - Belović, Miona
AU  - Torbica, Aleksandra
AU  - Zlatanović, Ivan
AU  - Pavlović, Vladimir
AU  - Onjia, Antonije
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6532
AB  - This study investigates the effects of ultrasound, in combination with chemical pretreatments, on the quality attributes (total phenolic and carotenoid content, antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl assay (DPPH)), ferric-reducing ability (FRAP), CIE L* a* b* color, non-enzymatic browning, rehydration ratio, textural and morphological properties) of red pepper subjected to drying (hot air drying or freeze drying). The fractional factorial design was used to assess the impact of factors. The global Derringer desirability function was used to determine the optimal conditions for the best quality attributes of dried pepper. The drying method influenced total phenolic content, a* (redness), and initial rehydration ratio; pretreatment time significantly affected FRAP antiradical activity, a*, chroma and non-browning index, while pH-value had a significant effect on the texture of dried pepper. Non-enzymatic browning was reduced to 72.6%, while the DPPH antioxidant capacity of freeze-dried peppers was enhanced from 4.2% to 71.9%. Ultrasonic pretreatment led to changes in the pepper morphology, while potassium metabisulfite (KMS) was a more effective additive than citric acid.
PB  - MDPI
T2  - Foods
T1  - Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)
IS  - 13
SP  - 2468
VL  - 12
DO  - 10.3390/foods12132468
ER  - 

@article{
author = "Lučić, Milica and Potkonjak, Nebojša and Sredović Ignjatović, Ivana and Lević, Steva and Dajić-Stevanović, Zora and Kolašinac, Stefan and Belović, Miona and Torbica, Aleksandra and Zlatanović, Ivan and Pavlović, Vladimir and Onjia, Antonije",
year = "2023",
abstract = "This study investigates the effects of ultrasound, in combination with chemical pretreatments, on the quality attributes (total phenolic and carotenoid content, antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl assay (DPPH)), ferric-reducing ability (FRAP), CIE L* a* b* color, non-enzymatic browning, rehydration ratio, textural and morphological properties) of red pepper subjected to drying (hot air drying or freeze drying). The fractional factorial design was used to assess the impact of factors. The global Derringer desirability function was used to determine the optimal conditions for the best quality attributes of dried pepper. The drying method influenced total phenolic content, a* (redness), and initial rehydration ratio; pretreatment time significantly affected FRAP antiradical activity, a*, chroma and non-browning index, while pH-value had a significant effect on the texture of dried pepper. Non-enzymatic browning was reduced to 72.6%, while the DPPH antioxidant capacity of freeze-dried peppers was enhanced from 4.2% to 71.9%. Ultrasonic pretreatment led to changes in the pepper morphology, while potassium metabisulfite (KMS) was a more effective additive than citric acid.",
publisher = "MDPI",
journal = "Foods",
title = "Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)",
number = "13",
pages = "2468",
volume = "12",
doi = "10.3390/foods12132468"
}

Lučić, M., Potkonjak, N., Sredović Ignjatović, I., Lević, S., Dajić-Stevanović, Z., Kolašinac, S., Belović, M., Torbica, A., Zlatanović, I., Pavlović, V.,& Onjia, A.. (2023). Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum). in Foods
MDPI., 12(13), 2468.
https://doi.org/10.3390/foods12132468

Lučić M, Potkonjak N, Sredović Ignjatović I, Lević S, Dajić-Stevanović Z, Kolašinac S, Belović M, Torbica A, Zlatanović I, Pavlović V, Onjia A. Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum). in Foods. 2023;12(13):2468.
doi:10.3390/foods12132468 .

Lučić, Milica, Potkonjak, Nebojša, Sredović Ignjatović, Ivana, Lević, Steva, Dajić-Stevanović, Zora, Kolašinac, Stefan, Belović, Miona, Torbica, Aleksandra, Zlatanović, Ivan, Pavlović, Vladimir, Onjia, Antonije, "Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum)" in Foods, 12, no. 13 (2023):2468,
https://doi.org/10.3390/foods12132468 . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Sieniawska, Elwira
AU  - Glowniak, Kazimierz
AU  - Obradović, Nataša
AU  - Pajić-Lijaković, Ivana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4517
AB  - Essential oils (EOs) and their main constituents, the terpenes, are widely studied, mostly relating to their antioxidant ability and bioactivity, such as antimicrobial, anticancer, anti-inflammatory, and range of other actions in the living systems. However, there is limited information on their bioavailability, especially upon clinical studies. Having in mind both strong biological effects and health benefits of EOs and their specific physicochemical properties (volatility, lipophilic character, low water solubility or insolubility, viscosity, expressed odor, concentration-dependent toxicity, etc.), there is a need for their encapsulation for target delivery. Encapsulation of EOs and their constituents is the prerequisite for enhancing their oxidative stability, thermostability, photostability, shelf life, and biological activity. We considered various carrier types such a (1) monophase and polyphase polysaccharide hydrogel carriers, (2) polysaccharide-protein carriers, and (3) lipid carriers in the context of physicochemical and engineering factors. Physicochemical factors are encapsulation efficiency, chemical stability under gastric conditions, mechanical stability, and thermal stability of carrier matrices. Choice of carrier material also determines the encapsulation technique. Consequently, the engineering factors are related to the advantage and disadvantage of various encapsulation techniques frequently used in the literature. In addition, it was intended to address the interactions between (1) main carrier components, such as polysaccharides, proteins, and lipids themselves (in order to form chemically and mechanically stable structure); (2) main carrier components with pepsin under gastric conditions (in order to form resistant material under gastric conditions); and (3) main carrier components with EOs (in order to enhance encapsulation efficiency), as a necessary precondition for whole process optimization. Finally, different sources for obtaining natural carrier macromolecules are surveyed, especially the agro-waste materials and agricultural and food by-products. This review article highlights the bioavailability aspects of encapsulated EOs and physicochemical and engineering factors concerning natural macromolecule carriers for their target delivery and application.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application
VL  - 8
DO  - 10.3389/fbioe.2020.00563
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Sieniawska, Elwira and Glowniak, Kazimierz and Obradović, Nataša and Pajić-Lijaković, Ivana",
year = "2020",
abstract = "Essential oils (EOs) and their main constituents, the terpenes, are widely studied, mostly relating to their antioxidant ability and bioactivity, such as antimicrobial, anticancer, anti-inflammatory, and range of other actions in the living systems. However, there is limited information on their bioavailability, especially upon clinical studies. Having in mind both strong biological effects and health benefits of EOs and their specific physicochemical properties (volatility, lipophilic character, low water solubility or insolubility, viscosity, expressed odor, concentration-dependent toxicity, etc.), there is a need for their encapsulation for target delivery. Encapsulation of EOs and their constituents is the prerequisite for enhancing their oxidative stability, thermostability, photostability, shelf life, and biological activity. We considered various carrier types such a (1) monophase and polyphase polysaccharide hydrogel carriers, (2) polysaccharide-protein carriers, and (3) lipid carriers in the context of physicochemical and engineering factors. Physicochemical factors are encapsulation efficiency, chemical stability under gastric conditions, mechanical stability, and thermal stability of carrier matrices. Choice of carrier material also determines the encapsulation technique. Consequently, the engineering factors are related to the advantage and disadvantage of various encapsulation techniques frequently used in the literature. In addition, it was intended to address the interactions between (1) main carrier components, such as polysaccharides, proteins, and lipids themselves (in order to form chemically and mechanically stable structure); (2) main carrier components with pepsin under gastric conditions (in order to form resistant material under gastric conditions); and (3) main carrier components with EOs (in order to enhance encapsulation efficiency), as a necessary precondition for whole process optimization. Finally, different sources for obtaining natural carrier macromolecules are surveyed, especially the agro-waste materials and agricultural and food by-products. This review article highlights the bioavailability aspects of encapsulated EOs and physicochemical and engineering factors concerning natural macromolecule carriers for their target delivery and application.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application",
volume = "8",
doi = "10.3389/fbioe.2020.00563"
}

Dajić-Stevanović, Z., Sieniawska, E., Glowniak, K., Obradović, N.,& Pajić-Lijaković, I.. (2020). Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fbioe.2020.00563

Dajić-Stevanović Z, Sieniawska E, Glowniak K, Obradović N, Pajić-Lijaković I. Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application. in Frontiers in Bioengineering and Biotechnology. 2020;8.
doi:10.3389/fbioe.2020.00563 .

Dajić-Stevanović, Zora, Sieniawska, Elwira, Glowniak, Kazimierz, Obradović, Nataša, Pajić-Lijaković, Ivana, "Natural Macromolecules as Carriers for Essential Oils: From Extraction to Biomedical Application" in Frontiers in Bioengineering and Biotechnology, 8 (2020),
https://doi.org/10.3389/fbioe.2020.00563 . .

TY  - CHAP
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
AU  - Lević, Steva
AU  - Trifković, Kata T.
AU  - Balanč, Bojana
AU  - Nedović, Viktor
AU  - Stevanović-Dajić, Zora
AU  - Radosević, Radenko
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4162
AB  - Some of the main rheological properties that Ca-alginate hydrogel matrix should satisfy for biomedical and biotechnological applications are the matrix viscoelasticity and the ability of stress relaxation. Although alginate satisfies both of them, experimental data note that cell growth is significantly reduced by microenvironmental effects. Microenvironmental restriction effects are connected to matrix resistance stress accumulation. Matrix stress is generated within the boundary layers around the cell aggregates under compression caused by cell rearrangement and growth. Simultaneously induced relaxation phenomena of both subsystems: (1) immobilized cells and (2) hydrogel matrix occur at three time scales through successive relaxation cycles. Complex dynamics of matrix compression intensifies mechanical and electrostatic cell-matrix interactions. Minimizing of the resistance stress is the strategy for improving the matrix performances. Cell action could be simulated in the experiments without cells during repeated stress relaxation cycles. We consider the physical and chemical modifications of Ca-alginate hydrogel.
T2  - Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications
T1  - Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells
EP  - 306
SP  - 281
DO  - 10.1016/B978-0-12-818431-8.00009-X
ER  - 

@inbook{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan and Lević, Steva and Trifković, Kata T. and Balanč, Bojana and Nedović, Viktor and Stevanović-Dajić, Zora and Radosević, Radenko and Bugarski, Branko",
year = "2019",
abstract = "Some of the main rheological properties that Ca-alginate hydrogel matrix should satisfy for biomedical and biotechnological applications are the matrix viscoelasticity and the ability of stress relaxation. Although alginate satisfies both of them, experimental data note that cell growth is significantly reduced by microenvironmental effects. Microenvironmental restriction effects are connected to matrix resistance stress accumulation. Matrix stress is generated within the boundary layers around the cell aggregates under compression caused by cell rearrangement and growth. Simultaneously induced relaxation phenomena of both subsystems: (1) immobilized cells and (2) hydrogel matrix occur at three time scales through successive relaxation cycles. Complex dynamics of matrix compression intensifies mechanical and electrostatic cell-matrix interactions. Minimizing of the resistance stress is the strategy for improving the matrix performances. Cell action could be simulated in the experiments without cells during repeated stress relaxation cycles. We consider the physical and chemical modifications of Ca-alginate hydrogel.",
journal = "Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications",
booktitle = "Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells",
pages = "306-281",
doi = "10.1016/B978-0-12-818431-8.00009-X"
}

Pajić-Lijaković, I., Milivojević, M., Lević, S., Trifković, K. T., Balanč, B., Nedović, V., Stevanović-Dajić, Z., Radosević, R.,& Bugarski, B.. (2019). Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells. in Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications, 281-306.
https://doi.org/10.1016/B978-0-12-818431-8.00009-X

Pajić-Lijaković I, Milivojević M, Lević S, Trifković KT, Balanč B, Nedović V, Stevanović-Dajić Z, Radosević R, Bugarski B. Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells. in Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications. 2019;:281-306.
doi:10.1016/B978-0-12-818431-8.00009-X .

Pajić-Lijaković, Ivana, Milivojević, Milan, Lević, Steva, Trifković, Kata T., Balanč, Bojana, Nedović, Viktor, Stevanović-Dajić, Zora, Radosević, Radenko, Bugarski, Branko, "Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells" in Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications (2019):281-306,
https://doi.org/10.1016/B978-0-12-818431-8.00009-X . .

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Bosnjak-Neumueller, Jasna
AU  - Pajić-Lijaković, Ivana
AU  - Raj, Jog
AU  - Vasiljević, Marko
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3838
AB  - The inconsistency of phytogenic feed additives' (PFA) effects on the livestock industry poses a risk for their use as a replacement for antibiotic growth promoters. The livestock market is being encouraged to use natural growth promotors, but information is limited about the PFA mode of action. The aim of this paper is to present the complexity of compounds present in essential oils (EOs) and factors that influence biological effects of PFA. In this paper, we highlight various controls and optimization parameters that influence the processes for the standardization of these products. The chemical composition of EOs depends on plant genetics, growth conditions, development stage at harvest, and processes of extracting active compounds. Their biological effects are further influenced by the interaction of phytochemicals and their bioavailability in the gastrointestinal tract of animals. PFA effects on animal health and production are also complex due to various EO antibiotic, antioxidant, anti-quorum sensing, anti-inflammatory, and digestive fluids stimulating activities. Research must focus on reliable methods to identify and control the quality and effects of EOs. In this study, we focused on available microencapsulation techniques of EOs to increase the bioavailability of active compounds, as well as their application in the animal feed additive industry.
PB  - MDPI, Basel
T2  - Molecules
T1  - Essential Oils as Feed AdditivesFuture Perspectives
IS  - 7
VL  - 23
DO  - 10.3390/molecules23071717
ER  - 

@article{
author = "Dajić-Stevanović, Zora and Bosnjak-Neumueller, Jasna and Pajić-Lijaković, Ivana and Raj, Jog and Vasiljević, Marko",
year = "2018",
abstract = "The inconsistency of phytogenic feed additives' (PFA) effects on the livestock industry poses a risk for their use as a replacement for antibiotic growth promoters. The livestock market is being encouraged to use natural growth promotors, but information is limited about the PFA mode of action. The aim of this paper is to present the complexity of compounds present in essential oils (EOs) and factors that influence biological effects of PFA. In this paper, we highlight various controls and optimization parameters that influence the processes for the standardization of these products. The chemical composition of EOs depends on plant genetics, growth conditions, development stage at harvest, and processes of extracting active compounds. Their biological effects are further influenced by the interaction of phytochemicals and their bioavailability in the gastrointestinal tract of animals. PFA effects on animal health and production are also complex due to various EO antibiotic, antioxidant, anti-quorum sensing, anti-inflammatory, and digestive fluids stimulating activities. Research must focus on reliable methods to identify and control the quality and effects of EOs. In this study, we focused on available microencapsulation techniques of EOs to increase the bioavailability of active compounds, as well as their application in the animal feed additive industry.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Essential Oils as Feed AdditivesFuture Perspectives",
number = "7",
volume = "23",
doi = "10.3390/molecules23071717"
}

Dajić-Stevanović, Z., Bosnjak-Neumueller, J., Pajić-Lijaković, I., Raj, J.,& Vasiljević, M.. (2018). Essential Oils as Feed AdditivesFuture Perspectives. in Molecules
MDPI, Basel., 23(7).
https://doi.org/10.3390/molecules23071717

Dajić-Stevanović Z, Bosnjak-Neumueller J, Pajić-Lijaković I, Raj J, Vasiljević M. Essential Oils as Feed AdditivesFuture Perspectives. in Molecules. 2018;23(7).
doi:10.3390/molecules23071717 .

Dajić-Stevanović, Zora, Bosnjak-Neumueller, Jasna, Pajić-Lijaković, Ivana, Raj, Jog, Vasiljević, Marko, "Essential Oils as Feed AdditivesFuture Perspectives" in Molecules, 23, no. 7 (2018),
https://doi.org/10.3390/molecules23071717 . .

TY  - JOUR
AU  - Volić, Mina
AU  - Pajić-Lijaković, Ivana
AU  - Đorđević, Verica
AU  - Knežević-Jugović, Zorica
AU  - Pecinar, Ilinka
AU  - Stevanović-Dajić, Zora
AU  - Veljović, Đorđe
AU  - Hadnadjev, Miroslav
AU  - Bugarski, Branko
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3851
AB  - Preparation of alginate-soy protein isolate (AL/SPI) complex beads containing essential oil of thyme was carried out by emulsification of thyme oil in aqueous sodium alginate solution blended with SPI solution, followed by atomization via electrostatic extrusion and gelification with calcium ions. The process parameters were optimized by variation of the alginate (1-2.5 wt.%) and SPI (0-1.5 wt.%) concentrations. Dry alginate-SPI particles exhibited wrinkle surface while shape distortion of hydrogel beads occurred with  gt = 1.5 wt.% alginate concentration, whereas SPI induced reduction of the particle size. Encapsulation efficiency of 72-80 % based on total polyphenols was achieved. In SGF the samples exhibited oil release of 42-55 % (due to matrix shrinkage and proteins degradation by pepsin activity), while the rest was delivered in SIF within 2.5 h simultaneously with swelling and degradation of the matrix.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Alginate/soy protein system for essential oil encapsulation with intestinal delivery
EP  - 24
SP  - 15
VL  - 200
DO  - 10.1016/j.carbpol.2018.07.033
ER  - 

@article{
author = "Volić, Mina and Pajić-Lijaković, Ivana and Đorđević, Verica and Knežević-Jugović, Zorica and Pecinar, Ilinka and Stevanović-Dajić, Zora and Veljović, Đorđe and Hadnadjev, Miroslav and Bugarski, Branko",
year = "2018",
abstract = "Preparation of alginate-soy protein isolate (AL/SPI) complex beads containing essential oil of thyme was carried out by emulsification of thyme oil in aqueous sodium alginate solution blended with SPI solution, followed by atomization via electrostatic extrusion and gelification with calcium ions. The process parameters were optimized by variation of the alginate (1-2.5 wt.%) and SPI (0-1.5 wt.%) concentrations. Dry alginate-SPI particles exhibited wrinkle surface while shape distortion of hydrogel beads occurred with  gt = 1.5 wt.% alginate concentration, whereas SPI induced reduction of the particle size. Encapsulation efficiency of 72-80 % based on total polyphenols was achieved. In SGF the samples exhibited oil release of 42-55 % (due to matrix shrinkage and proteins degradation by pepsin activity), while the rest was delivered in SIF within 2.5 h simultaneously with swelling and degradation of the matrix.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Alginate/soy protein system for essential oil encapsulation with intestinal delivery",
pages = "24-15",
volume = "200",
doi = "10.1016/j.carbpol.2018.07.033"
}

Volić, M., Pajić-Lijaković, I., Đorđević, V., Knežević-Jugović, Z., Pecinar, I., Stevanović-Dajić, Z., Veljović, Đ., Hadnadjev, M.,& Bugarski, B.. (2018). Alginate/soy protein system for essential oil encapsulation with intestinal delivery. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 200, 15-24.
https://doi.org/10.1016/j.carbpol.2018.07.033

Volić M, Pajić-Lijaković I, Đorđević V, Knežević-Jugović Z, Pecinar I, Stevanović-Dajić Z, Veljović Đ, Hadnadjev M, Bugarski B. Alginate/soy protein system for essential oil encapsulation with intestinal delivery. in Carbohydrate Polymers. 2018;200:15-24.
doi:10.1016/j.carbpol.2018.07.033 .

Volić, Mina, Pajić-Lijaković, Ivana, Đorđević, Verica, Knežević-Jugović, Zorica, Pecinar, Ilinka, Stevanović-Dajić, Zora, Veljović, Đorđe, Hadnadjev, Miroslav, Bugarski, Branko, "Alginate/soy protein system for essential oil encapsulation with intestinal delivery" in Carbohydrate Polymers, 200 (2018):15-24,
https://doi.org/10.1016/j.carbpol.2018.07.033 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Milivojević, Milan
AU  - Lević, Steva
AU  - Trifković, Kata T.
AU  - Stevanović-Dajić, Zora
AU  - Radosević, Radenko
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3666
AB  - Microenvironmentally restricted yeast cell growth within Ca-alginate beads with and without entrapped gas bubbles was considered based on experimental data. Cell growth dynamics was described by (1) the dimensionless cell number density as a function of the cell growth time and (2) the cell distribution per bead cross sections. One of the key control parameters for bioprocess optimization is the matrix resistance stress generated during immobilized cell expansion. The dynamics of the increase in matrix stress was described theoretically based on a multi-scale mathematical model. In order to estimate and reduce the accumulation of matrix stress we considered repeated stress relaxation cycles in separate rheological experiments without immobilized cells. The results revealed that the increase in resistance stress within the Ca-alginate matrix was significant (similar to 7 kPa) after 10 repeated cycles, even under a low compression strain of 2% per cycle. The stress could be reduced by using the Ca-alginate matrix with entrapped gas bubbles. The final cell concentration within the beads with entrapped bubbles was 3.3 times higher in comparison with the beads without bubbles. The bubbles could locally amortize the compression effects within the surrounding cell clusters.
PB  - Elsevier Sci Ltd, Oxford
T2  - Process Biochemistry
T1  - Matrix resistance stress: A key parameter for immobilized cell growth regulation
EP  - 43
SP  - 30
VL  - 52
DO  - 10.1016/j.procbio.2016.10.017
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Milivojević, Milan and Lević, Steva and Trifković, Kata T. and Stevanović-Dajić, Zora and Radosević, Radenko and Nedović, Viktor and Bugarski, Branko",
year = "2017",
abstract = "Microenvironmentally restricted yeast cell growth within Ca-alginate beads with and without entrapped gas bubbles was considered based on experimental data. Cell growth dynamics was described by (1) the dimensionless cell number density as a function of the cell growth time and (2) the cell distribution per bead cross sections. One of the key control parameters for bioprocess optimization is the matrix resistance stress generated during immobilized cell expansion. The dynamics of the increase in matrix stress was described theoretically based on a multi-scale mathematical model. In order to estimate and reduce the accumulation of matrix stress we considered repeated stress relaxation cycles in separate rheological experiments without immobilized cells. The results revealed that the increase in resistance stress within the Ca-alginate matrix was significant (similar to 7 kPa) after 10 repeated cycles, even under a low compression strain of 2% per cycle. The stress could be reduced by using the Ca-alginate matrix with entrapped gas bubbles. The final cell concentration within the beads with entrapped bubbles was 3.3 times higher in comparison with the beads without bubbles. The bubbles could locally amortize the compression effects within the surrounding cell clusters.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Process Biochemistry",
title = "Matrix resistance stress: A key parameter for immobilized cell growth regulation",
pages = "43-30",
volume = "52",
doi = "10.1016/j.procbio.2016.10.017"
}

Pajić-Lijaković, I., Milivojević, M., Lević, S., Trifković, K. T., Stevanović-Dajić, Z., Radosević, R., Nedović, V.,& Bugarski, B.. (2017). Matrix resistance stress: A key parameter for immobilized cell growth regulation. in Process Biochemistry
Elsevier Sci Ltd, Oxford., 52, 30-43.
https://doi.org/10.1016/j.procbio.2016.10.017

Pajić-Lijaković I, Milivojević M, Lević S, Trifković KT, Stevanović-Dajić Z, Radosević R, Nedović V, Bugarski B. Matrix resistance stress: A key parameter for immobilized cell growth regulation. in Process Biochemistry. 2017;52:30-43.
doi:10.1016/j.procbio.2016.10.017 .

Pajić-Lijaković, Ivana, Milivojević, Milan, Lević, Steva, Trifković, Kata T., Stevanović-Dajić, Zora, Radosević, Radenko, Nedović, Viktor, Bugarski, Branko, "Matrix resistance stress: A key parameter for immobilized cell growth regulation" in Process Biochemistry, 52 (2017):30-43,
https://doi.org/10.1016/j.procbio.2016.10.017 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Lević, Steva
AU  - Hadnadjev, Miroslav
AU  - Stevanović-Dajić, Zora
AU  - Radosević, Radenko
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3140
AB  - Structural changes of Ca-alginate bead due to yeast cell loading and the impact on their mechanical properties were studied based on experimental data of cell number density per beads, cell rearrangement within the bead surface and core regions, bead average volume and density, bead storage and loss moduli. Structural changes could be explained by (1) mechanical and electrostatic cell-matrix interactions and (2) chemical interactions of the matrix with the components of the nutrition medium which include: wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate. According to obtained results, it was estimated that: (1) cell-matrix electrostatic interactions within the hydrogel matrix at t=0 induced the bead weakening, (2) wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate led to the bead weakening (regime 1, t is an element of (left perpendicular0, 2 daysright perpendicular), (3) the bead stiffness remained constant (regime 2, t is an element of (left perpendicular2, 4 daysright perpendicular)) and (4) the bead reinforcement was caused by cell clusters rapid increase and their inter connections within the bead surface region (regime 3, t is an element of (left perpendicular4, 5 daysright perpendicular)). Regulation of the matrix resistance stress and the rate of its change could lead to decrease of the micro-environmental restriction effects and the whole process optimization.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochemical Engineering Journal
T1  - Structural changes of Ca-alginate beads caused by immobilized yeast cell growth
EP  - 38
SP  - 32
VL  - 103
DO  - 10.1016/j.bej.2015.06.016
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Lević, Steva and Hadnadjev, Miroslav and Stevanović-Dajić, Zora and Radosević, Radenko and Nedović, Viktor and Bugarski, Branko",
year = "2015",
abstract = "Structural changes of Ca-alginate bead due to yeast cell loading and the impact on their mechanical properties were studied based on experimental data of cell number density per beads, cell rearrangement within the bead surface and core regions, bead average volume and density, bead storage and loss moduli. Structural changes could be explained by (1) mechanical and electrostatic cell-matrix interactions and (2) chemical interactions of the matrix with the components of the nutrition medium which include: wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate. According to obtained results, it was estimated that: (1) cell-matrix electrostatic interactions within the hydrogel matrix at t=0 induced the bead weakening, (2) wash out of Ca2+ ions by presence of non-gelling ions and by chelating compounds such as phosphate led to the bead weakening (regime 1, t is an element of (left perpendicular0, 2 daysright perpendicular), (3) the bead stiffness remained constant (regime 2, t is an element of (left perpendicular2, 4 daysright perpendicular)) and (4) the bead reinforcement was caused by cell clusters rapid increase and their inter connections within the bead surface region (regime 3, t is an element of (left perpendicular4, 5 daysright perpendicular)). Regulation of the matrix resistance stress and the rate of its change could lead to decrease of the micro-environmental restriction effects and the whole process optimization.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochemical Engineering Journal",
title = "Structural changes of Ca-alginate beads caused by immobilized yeast cell growth",
pages = "38-32",
volume = "103",
doi = "10.1016/j.bej.2015.06.016"
}

Pajić-Lijaković, I., Lević, S., Hadnadjev, M., Stevanović-Dajić, Z., Radosević, R., Nedović, V.,& Bugarski, B.. (2015). Structural changes of Ca-alginate beads caused by immobilized yeast cell growth. in Biochemical Engineering Journal
Elsevier Science Bv, Amsterdam., 103, 32-38.
https://doi.org/10.1016/j.bej.2015.06.016

Pajić-Lijaković I, Lević S, Hadnadjev M, Stevanović-Dajić Z, Radosević R, Nedović V, Bugarski B. Structural changes of Ca-alginate beads caused by immobilized yeast cell growth. in Biochemical Engineering Journal. 2015;103:32-38.
doi:10.1016/j.bej.2015.06.016 .

Pajić-Lijaković, Ivana, Lević, Steva, Hadnadjev, Miroslav, Stevanović-Dajić, Zora, Radosević, Radenko, Nedović, Viktor, Bugarski, Branko, "Structural changes of Ca-alginate beads caused by immobilized yeast cell growth" in Biochemical Engineering Journal, 103 (2015):32-38,
https://doi.org/10.1016/j.bej.2015.06.016 . .