TY  - JOUR
AU  - Simović, Ljiljana
AU  - Škundrić, Petar
AU  - Kostić, Mirjana
AU  - Tasić, Goran
AU  - Kojić, Zvezdana Z.
AU  - Milaković, Branko D.
AU  - Medović, Adela
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1948
AB  - the antimicrobial textile material. The material is in the form of plaster wound dressing which is consisting of a nonwoven textile base containing polypropylene (PP)/viscose, a polymer carrier, and an antimicrobial active substance. The polymer carrier is the polysaccharide D-glucosamine (chitosan), and the active antimicrobial substance is antibiotic gentamicin sulfate. The amount of gentamicin sulfate immobilized into the polymer matrix of the polymer carrier was 0.15-0.20 mg/cm(2) or 2.0-2.5% of the mass of the nonwoven textile material. The antimicrobial textile material has been studied in vitro and in vivo conditions through the efficiency of the antimicrobial effects on different kinds of pathogenic microorganisms, as well as the biocompatibility in preclinical research. The results of these experiments indicate that all bioactive textile materials were biocidal in vitro for all pathogenic test organisms. Good biocompatibility, the existence of the correlation between the in vitro and in vivo results, concerning efficiency, qualifies these antimicrobial biornaterials for clinical use.
PB  - Wiley, Hoboken
T2  - Journal of Applied Polymer Science
T1  - Efficiency and Biocompatibility of Antimicrobial Textile Material of Broad Spectrum Activity
EP  - 1467
IS  - 3
SP  - 1459
VL  - 120
DO  - 10.1002/app.33235
ER  - 

@article{
author = "Simović, Ljiljana and Škundrić, Petar and Kostić, Mirjana and Tasić, Goran and Kojić, Zvezdana Z. and Milaković, Branko D. and Medović, Adela",
year = "2011",
abstract = "the antimicrobial textile material. The material is in the form of plaster wound dressing which is consisting of a nonwoven textile base containing polypropylene (PP)/viscose, a polymer carrier, and an antimicrobial active substance. The polymer carrier is the polysaccharide D-glucosamine (chitosan), and the active antimicrobial substance is antibiotic gentamicin sulfate. The amount of gentamicin sulfate immobilized into the polymer matrix of the polymer carrier was 0.15-0.20 mg/cm(2) or 2.0-2.5% of the mass of the nonwoven textile material. The antimicrobial textile material has been studied in vitro and in vivo conditions through the efficiency of the antimicrobial effects on different kinds of pathogenic microorganisms, as well as the biocompatibility in preclinical research. The results of these experiments indicate that all bioactive textile materials were biocidal in vitro for all pathogenic test organisms. Good biocompatibility, the existence of the correlation between the in vitro and in vivo results, concerning efficiency, qualifies these antimicrobial biornaterials for clinical use.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Polymer Science",
title = "Efficiency and Biocompatibility of Antimicrobial Textile Material of Broad Spectrum Activity",
pages = "1467-1459",
number = "3",
volume = "120",
doi = "10.1002/app.33235"
}

Simović, L., Škundrić, P., Kostić, M., Tasić, G., Kojić, Z. Z., Milaković, B. D.,& Medović, A.. (2011). Efficiency and Biocompatibility of Antimicrobial Textile Material of Broad Spectrum Activity. in Journal of Applied Polymer Science
Wiley, Hoboken., 120(3), 1459-1467.
https://doi.org/10.1002/app.33235

Simović L, Škundrić P, Kostić M, Tasić G, Kojić ZZ, Milaković BD, Medović A. Efficiency and Biocompatibility of Antimicrobial Textile Material of Broad Spectrum Activity. in Journal of Applied Polymer Science. 2011;120(3):1459-1467.
doi:10.1002/app.33235 .

Simović, Ljiljana, Škundrić, Petar, Kostić, Mirjana, Tasić, Goran, Kojić, Zvezdana Z., Milaković, Branko D., Medović, Adela, "Efficiency and Biocompatibility of Antimicrobial Textile Material of Broad Spectrum Activity" in Journal of Applied Polymer Science, 120, no. 3 (2011):1459-1467,
https://doi.org/10.1002/app.33235 . .

TY  - JOUR
AU  - Simović, Ljiljana
AU  - Škundrić, Petar
AU  - Pajić-Lijaković, Ivana
AU  - Ristić, Katarina
AU  - Medović, Adela
AU  - Tasić, Goran
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1606
AB  - A mathematical model was developed to estimate the release of gentamicin sulfate from a bioactive textile material as a transdermal system for wound dressing. The gentamicin sulfate released from the antibiotic/chitosan hydrogel complexes was measured in vitro by the Franz diffusion cell technique. The diffusive transport of gentamicin sulfate through three connected compartments, that is, chitosan hydrogel, membrane, and solution, was considered by the formulation of a model based on Fick's second law. Initially, the total amount of gentamicin sulfate was placed within an already swollen chitosan hydrogel. The value of the diffusivity coefficient of the drug through the chitosan hydrogel was calculated for every initial amount of the active substance. For the initial concentration of gentamicin sulfate, which was lower than 2.81 x 10(4) mu g/mL, the diffusion coefficient was approximately constant. A higher amount of gentamicin sulfate in the hydrogel reduced its own transport as a consequence of an increase in the intensity of the interaction field between the molecules of gentamicin sulfate. The model provides the possibility of optimizing the process of drug release by ensuring a compromise between a higher value of the diffusivity coefficient and a desirable amount of gentamicin sulfate and a constant concentration within the solution over 48 h.
PB  - John Wiley & Sons Inc, Hoboken
T2  - Journal of Applied Polymer Science
T1  - Mathematical Model of Gentamicin Sulfate Release from a Bioactive Textile Material as a Transdermal System Under In Vitro Conditions
EP  - 1430
IS  - 3
SP  - 1424
VL  - 117
DO  - 10.1002/app.31964
ER  - 

@article{
author = "Simović, Ljiljana and Škundrić, Petar and Pajić-Lijaković, Ivana and Ristić, Katarina and Medović, Adela and Tasić, Goran",
year = "2010",
abstract = "A mathematical model was developed to estimate the release of gentamicin sulfate from a bioactive textile material as a transdermal system for wound dressing. The gentamicin sulfate released from the antibiotic/chitosan hydrogel complexes was measured in vitro by the Franz diffusion cell technique. The diffusive transport of gentamicin sulfate through three connected compartments, that is, chitosan hydrogel, membrane, and solution, was considered by the formulation of a model based on Fick's second law. Initially, the total amount of gentamicin sulfate was placed within an already swollen chitosan hydrogel. The value of the diffusivity coefficient of the drug through the chitosan hydrogel was calculated for every initial amount of the active substance. For the initial concentration of gentamicin sulfate, which was lower than 2.81 x 10(4) mu g/mL, the diffusion coefficient was approximately constant. A higher amount of gentamicin sulfate in the hydrogel reduced its own transport as a consequence of an increase in the intensity of the interaction field between the molecules of gentamicin sulfate. The model provides the possibility of optimizing the process of drug release by ensuring a compromise between a higher value of the diffusivity coefficient and a desirable amount of gentamicin sulfate and a constant concentration within the solution over 48 h.",
publisher = "John Wiley & Sons Inc, Hoboken",
journal = "Journal of Applied Polymer Science",
title = "Mathematical Model of Gentamicin Sulfate Release from a Bioactive Textile Material as a Transdermal System Under In Vitro Conditions",
pages = "1430-1424",
number = "3",
volume = "117",
doi = "10.1002/app.31964"
}

Simović, L., Škundrić, P., Pajić-Lijaković, I., Ristić, K., Medović, A.,& Tasić, G.. (2010). Mathematical Model of Gentamicin Sulfate Release from a Bioactive Textile Material as a Transdermal System Under In Vitro Conditions. in Journal of Applied Polymer Science
John Wiley & Sons Inc, Hoboken., 117(3), 1424-1430.
https://doi.org/10.1002/app.31964

Simović L, Škundrić P, Pajić-Lijaković I, Ristić K, Medović A, Tasić G. Mathematical Model of Gentamicin Sulfate Release from a Bioactive Textile Material as a Transdermal System Under In Vitro Conditions. in Journal of Applied Polymer Science. 2010;117(3):1424-1430.
doi:10.1002/app.31964 .

Simović, Ljiljana, Škundrić, Petar, Pajić-Lijaković, Ivana, Ristić, Katarina, Medović, Adela, Tasić, Goran, "Mathematical Model of Gentamicin Sulfate Release from a Bioactive Textile Material as a Transdermal System Under In Vitro Conditions" in Journal of Applied Polymer Science, 117, no. 3 (2010):1424-1430,
https://doi.org/10.1002/app.31964 . .

TY  - JOUR
AU  - Stojanović, D.
AU  - Janaćković, Đorđe
AU  - Marković, Danica
AU  - Tasić, Goran
AU  - Aleksandrić, B.
AU  - Kojić, Zvezdana
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1255
AB  - Calcium phosphate ceramics are among the more commonly used and biocompatible ceramics. Recently, we have synthesized a new calcium phosphate ceramic, alpha tricalcium phosphate (aTCP). The aim of this study was to assess the biocompatibility of this original, in our laboratory modified, newly synthesized aTCP ceramic, by carefully evaluating the inflammatory reaction of soft tissue in response to its subcutaneous implantation, and by comparing this result with the results of already widely used virtually non-toxic, non-immunogenic, and almost chemically inert dental (DC) and hydroxy apatite (HAP) ceramics. Implants (diameter 5x2 mm) of aTCP, DC and HAP were implanted into 12 adult male rats subcutaneously. At 2 weeks and 12 weeks post-implantation, the animals were euthanized and the tissueimplant reactions were analyzed histologically. Evaluation of routine stained sections (5 μm, hematoxylin & eosin) of the cutis and subcutis surrounding the aTCP, DC and HAP ceramics revealed the following: 1) all the ceramic devices had fibrous connective tissue capsules; 2) there were significant differences in the tissue - implant reactions based on the estimated time, while there were no differences in the tissue-implant reaction based on the type of ceramic material; 3) two weeks after implantation hyperemia and cellular proliferation were the most expressive results, while twelve weeks after implantation extensive angiogenesis and collagen fibers production were the prominent findings for all types of implanted calcium phosphate ceramics.
AB  - Podaci iz literature ukazuju da se kalcijum fosfatna keramika ubraja među najčešće korišćene i biološki najkompatibilnije keramičke materijale koji se danas primenjuju u medicini. U našoj laboratoriji sintetisana je nova vrsta kalcijum fosfatne keramike, alfa-trikalcijum fosfat (aTCP). Cilj ovog rada je bio da se proceni biokompatibilnost ovog, modifikovanog, novosintetisanog aTCP, putem pažljive procene inflamatorne reakcije mekog tkiva koja nastaje kao odgovor na njegovu subkutanu implantaciju i putem upoređivanja ovih rezultata sa rezultatima subku- tane implantacije dentalne keramike (DC) i hidroksiapatita (HAP), koji su ne-toksični, ne-imunogeni i skoro hemijski potpuno inertni. Kod 12 odraslih mužjaka Wistar pacova implantirani su subkutano implanti aTCP, DC i HAP (disk 5x2 mm). Na kraju 2. i 12. nedelje nakon implantacije, životinje su žrtvovane i reakcija tkivoimplant je analizirana histološki. Analiza rutinski obojenih (5 μm, hemotoksilin & eozin) isečaka tkiva kutisa i subkutisa koje se nalazilo oko implantiranog aTCP, DC i HAP, otkrila je sledeće: 1) svi keramički implanti bili su okruženi fibroznom kapsulom; 2) u odnosu na vreme posle implantacije, postojale su značajne razlike u tkivo-implant reakciji, dok u odnosu na vrstu keramičkog materijala, nisu postojale razlike u reakciji tkiva na implant; 3) za sve tri vrste implantirane kalcijum-fosfatne keramike, glavni nalaz u tkivu bio je, dve nedelje nakon implantacije, hiperemija i ćelijska infiltracija, dok nakon 12 nedelja prisutna intenzivna angiogeneza i stvaranje kolagenih vlakana.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria, Beograd
T1  - A tissue-implant reaction associated with subcutan implantation of alpha-tricalcium phosphate, dental ceramic and hydroxyapatite bioceramics in rats
T1  - Tkivo-implant reakcija povezana sa subkutanom implantacijom alfa-trikalcijum fosfata, dentalne keramike i hidroksiapatit biokeramike kod pacova
EP  - 393
IS  - 4
SP  - 381
VL  - 58
DO  - 10.2298/AVB0804381S
ER  - 

@article{
author = "Stojanović, D. and Janaćković, Đorđe and Marković, Danica and Tasić, Goran and Aleksandrić, B. and Kojić, Zvezdana",
year = "2008",
abstract = "Calcium phosphate ceramics are among the more commonly used and biocompatible ceramics. Recently, we have synthesized a new calcium phosphate ceramic, alpha tricalcium phosphate (aTCP). The aim of this study was to assess the biocompatibility of this original, in our laboratory modified, newly synthesized aTCP ceramic, by carefully evaluating the inflammatory reaction of soft tissue in response to its subcutaneous implantation, and by comparing this result with the results of already widely used virtually non-toxic, non-immunogenic, and almost chemically inert dental (DC) and hydroxy apatite (HAP) ceramics. Implants (diameter 5x2 mm) of aTCP, DC and HAP were implanted into 12 adult male rats subcutaneously. At 2 weeks and 12 weeks post-implantation, the animals were euthanized and the tissueimplant reactions were analyzed histologically. Evaluation of routine stained sections (5 μm, hematoxylin & eosin) of the cutis and subcutis surrounding the aTCP, DC and HAP ceramics revealed the following: 1) all the ceramic devices had fibrous connective tissue capsules; 2) there were significant differences in the tissue - implant reactions based on the estimated time, while there were no differences in the tissue-implant reaction based on the type of ceramic material; 3) two weeks after implantation hyperemia and cellular proliferation were the most expressive results, while twelve weeks after implantation extensive angiogenesis and collagen fibers production were the prominent findings for all types of implanted calcium phosphate ceramics., Podaci iz literature ukazuju da se kalcijum fosfatna keramika ubraja među najčešće korišćene i biološki najkompatibilnije keramičke materijale koji se danas primenjuju u medicini. U našoj laboratoriji sintetisana je nova vrsta kalcijum fosfatne keramike, alfa-trikalcijum fosfat (aTCP). Cilj ovog rada je bio da se proceni biokompatibilnost ovog, modifikovanog, novosintetisanog aTCP, putem pažljive procene inflamatorne reakcije mekog tkiva koja nastaje kao odgovor na njegovu subkutanu implantaciju i putem upoređivanja ovih rezultata sa rezultatima subku- tane implantacije dentalne keramike (DC) i hidroksiapatita (HAP), koji su ne-toksični, ne-imunogeni i skoro hemijski potpuno inertni. Kod 12 odraslih mužjaka Wistar pacova implantirani su subkutano implanti aTCP, DC i HAP (disk 5x2 mm). Na kraju 2. i 12. nedelje nakon implantacije, životinje su žrtvovane i reakcija tkivoimplant je analizirana histološki. Analiza rutinski obojenih (5 μm, hemotoksilin & eozin) isečaka tkiva kutisa i subkutisa koje se nalazilo oko implantiranog aTCP, DC i HAP, otkrila je sledeće: 1) svi keramički implanti bili su okruženi fibroznom kapsulom; 2) u odnosu na vreme posle implantacije, postojale su značajne razlike u tkivo-implant reakciji, dok u odnosu na vrstu keramičkog materijala, nisu postojale razlike u reakciji tkiva na implant; 3) za sve tri vrste implantirane kalcijum-fosfatne keramike, glavni nalaz u tkivu bio je, dve nedelje nakon implantacije, hiperemija i ćelijska infiltracija, dok nakon 12 nedelja prisutna intenzivna angiogeneza i stvaranje kolagenih vlakana.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria, Beograd",
title = "A tissue-implant reaction associated with subcutan implantation of alpha-tricalcium phosphate, dental ceramic and hydroxyapatite bioceramics in rats, Tkivo-implant reakcija povezana sa subkutanom implantacijom alfa-trikalcijum fosfata, dentalne keramike i hidroksiapatit biokeramike kod pacova",
pages = "393-381",
number = "4",
volume = "58",
doi = "10.2298/AVB0804381S"
}

Stojanović, D., Janaćković, Đ., Marković, D., Tasić, G., Aleksandrić, B.,& Kojić, Z.. (2008). A tissue-implant reaction associated with subcutan implantation of alpha-tricalcium phosphate, dental ceramic and hydroxyapatite bioceramics in rats. in Acta Veterinaria, Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(4), 381-393.
https://doi.org/10.2298/AVB0804381S

Stojanović D, Janaćković Đ, Marković D, Tasić G, Aleksandrić B, Kojić Z. A tissue-implant reaction associated with subcutan implantation of alpha-tricalcium phosphate, dental ceramic and hydroxyapatite bioceramics in rats. in Acta Veterinaria, Beograd. 2008;58(4):381-393.
doi:10.2298/AVB0804381S .

Stojanović, D., Janaćković, Đorđe, Marković, Danica, Tasić, Goran, Aleksandrić, B., Kojić, Zvezdana, "A tissue-implant reaction associated with subcutan implantation of alpha-tricalcium phosphate, dental ceramic and hydroxyapatite bioceramics in rats" in Acta Veterinaria, Beograd, 58, no. 4 (2008):381-393,
https://doi.org/10.2298/AVB0804381S . .

TY  - JOUR
AU  - Medović, Adela
AU  - Kojić, Zvezdana
AU  - Tasić, Goran
AU  - Milaković, Branko
AU  - Kostić, Mirjana
AU  - Škundrić, Petar
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1263
AB  - In this paper an account of biocompatibility of a hormonactive fiber in the form of biologically active complex chitosan-insulin is presented. The behavior of artificial depots of insulin containing 180-330mg of insulin per gram of fiber has been studied. The artificial depot of insulin has been characterized from the standpoint of its behavior in physiological solution (in the culture of L929 cells and in organism of an experimental animal). In vitro conditions, the intensity of desorption depends on the nature of solution and on pH of medium. It has been shown that insulin is controllably delivered in physiological solutions in quantities of 1.3-1.6mg of insulin during 24 hours, at pH about 7. The quantity of insulin delivered has been assessed by UV Sprectrophotometry. Biocompatibility of hormone-active fibers in the form of artificial depot of insulin has been determined in pre-clinical conditions through determination of the degree of harmfulness (toxicity) to the organism of the experimental animal. Tests have been performed according to the matrix for testing of biocompatibility and according to recommendations and guides published in Guidelines ISO 10993. Noxiousness has been assessed by testing: citotoxicity, sensitivization and irritation. Qualitative and quantitative citotoxicity tests in direct contact on L929 cells culture have shown that a hormone-active fiber does not provoke the citotoxic effect. The results obtained have shown that quantities of controllably released insulin could satisfy basal needs of an organism and that the artificial depot does not show effects of toxicity. On the bases of the obtained results from tests and calculated index of sensitivitization it follows that the artificial depot of insulin does not cause sensitivization. Also, the results obtained by subcutaneous irritation testing showed that the fibrous artificial depot of insulin could be eveluated as a non-irritating bioactive material.
AB  - U ovom radu kao deo pretkliničkog ispitivanja biokompatibilnosti, ispitivana je toksičnost i efikasnost veštačkih depoa insulina. Veštački depoi insulina sadržali su 180-330 mg insulina po gramu vlakna hitozana. Cilj rada je bio: 1. odrediti kinetiku otpuštanja insulina iz hormonaktivnog vlakna u in vitro uslovima, 2. ispitati senzibilizaciju in vivo; 3. ispitati primarnu kutanu iritaciju in vivo i 4. ispitati citotoksični efekat in vitro. Metode: Količina otpuštenog insulina praćena je UV spektrofotometrijom. Pretklinička ispitivanja biokompatibilnosti hormonaktivnih vlakana izvršena su prema preporukama i vodičima publikovanih u Guidelines ISO 10993 kao i prema matriksu za ispitivanje biokompatibilnosti. Toksičnost je ispitana kroz testiranje: senzibilizacije i primarne kutane iritacije in vivo i testiranje citotoksičnosti (kvalitativno i kvantitativno) u kulturi L929 ćelija. Pokazano je da se insulin kontrolisano otpušta u fiziološkom rastvoru u količinama od 1.3-1.6 mg insulina u toku 24 sata, pri pH oko 7. Prema dobijenim rezultatima ispitivanja i izračunatog indeksa senzibilizcije sledi da veštački depo ne izaziva senzibilizaciju. Takođe, dobijeni rezultati ispitivanja primarne kutane iritacije su pokazali da se vlaknasti veštački depo insulina može oceniti kao neiritirajući bioaktivni materijal. Kvalitativni i kvantitativni testovi citotoksičnosti u direktnom kontaktu na kulturi ćelija L929 su pokazali da hormonaktivno vlakno ne izaziva citotoksični efekat. U cilju dalje karakterizacije biokompatibilnosti (toksičnosti i efikasnosti) hormonaktivnih polisahaidnih vlakana, dalja istraživanja su neophodna pre njihove kliničke primene.
PB  - Univerzitet u Beogradu - Medicinski fakultet, Beograd
T2  - Medicinska istraživanja
T1  - Toksičnost i kinetika otpuštanja insulina iz hormonaktivnih polisaharidnih vlakana
EP  - 22
IS  - 2
SP  - 17
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1263
ER  - 

@article{
author = "Medović, Adela and Kojić, Zvezdana and Tasić, Goran and Milaković, Branko and Kostić, Mirjana and Škundrić, Petar",
year = "2008",
abstract = "In this paper an account of biocompatibility of a hormonactive fiber in the form of biologically active complex chitosan-insulin is presented. The behavior of artificial depots of insulin containing 180-330mg of insulin per gram of fiber has been studied. The artificial depot of insulin has been characterized from the standpoint of its behavior in physiological solution (in the culture of L929 cells and in organism of an experimental animal). In vitro conditions, the intensity of desorption depends on the nature of solution and on pH of medium. It has been shown that insulin is controllably delivered in physiological solutions in quantities of 1.3-1.6mg of insulin during 24 hours, at pH about 7. The quantity of insulin delivered has been assessed by UV Sprectrophotometry. Biocompatibility of hormone-active fibers in the form of artificial depot of insulin has been determined in pre-clinical conditions through determination of the degree of harmfulness (toxicity) to the organism of the experimental animal. Tests have been performed according to the matrix for testing of biocompatibility and according to recommendations and guides published in Guidelines ISO 10993. Noxiousness has been assessed by testing: citotoxicity, sensitivization and irritation. Qualitative and quantitative citotoxicity tests in direct contact on L929 cells culture have shown that a hormone-active fiber does not provoke the citotoxic effect. The results obtained have shown that quantities of controllably released insulin could satisfy basal needs of an organism and that the artificial depot does not show effects of toxicity. On the bases of the obtained results from tests and calculated index of sensitivitization it follows that the artificial depot of insulin does not cause sensitivization. Also, the results obtained by subcutaneous irritation testing showed that the fibrous artificial depot of insulin could be eveluated as a non-irritating bioactive material., U ovom radu kao deo pretkliničkog ispitivanja biokompatibilnosti, ispitivana je toksičnost i efikasnost veštačkih depoa insulina. Veštački depoi insulina sadržali su 180-330 mg insulina po gramu vlakna hitozana. Cilj rada je bio: 1. odrediti kinetiku otpuštanja insulina iz hormonaktivnog vlakna u in vitro uslovima, 2. ispitati senzibilizaciju in vivo; 3. ispitati primarnu kutanu iritaciju in vivo i 4. ispitati citotoksični efekat in vitro. Metode: Količina otpuštenog insulina praćena je UV spektrofotometrijom. Pretklinička ispitivanja biokompatibilnosti hormonaktivnih vlakana izvršena su prema preporukama i vodičima publikovanih u Guidelines ISO 10993 kao i prema matriksu za ispitivanje biokompatibilnosti. Toksičnost je ispitana kroz testiranje: senzibilizacije i primarne kutane iritacije in vivo i testiranje citotoksičnosti (kvalitativno i kvantitativno) u kulturi L929 ćelija. Pokazano je da se insulin kontrolisano otpušta u fiziološkom rastvoru u količinama od 1.3-1.6 mg insulina u toku 24 sata, pri pH oko 7. Prema dobijenim rezultatima ispitivanja i izračunatog indeksa senzibilizcije sledi da veštački depo ne izaziva senzibilizaciju. Takođe, dobijeni rezultati ispitivanja primarne kutane iritacije su pokazali da se vlaknasti veštački depo insulina može oceniti kao neiritirajući bioaktivni materijal. Kvalitativni i kvantitativni testovi citotoksičnosti u direktnom kontaktu na kulturi ćelija L929 su pokazali da hormonaktivno vlakno ne izaziva citotoksični efekat. U cilju dalje karakterizacije biokompatibilnosti (toksičnosti i efikasnosti) hormonaktivnih polisahaidnih vlakana, dalja istraživanja su neophodna pre njihove kliničke primene.",
publisher = "Univerzitet u Beogradu - Medicinski fakultet, Beograd",
journal = "Medicinska istraživanja",
title = "Toksičnost i kinetika otpuštanja insulina iz hormonaktivnih polisaharidnih vlakana",
pages = "22-17",
number = "2",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1263"
}

Medović, A., Kojić, Z., Tasić, G., Milaković, B., Kostić, M.,& Škundrić, P.. (2008). Toksičnost i kinetika otpuštanja insulina iz hormonaktivnih polisaharidnih vlakana. in Medicinska istraživanja
Univerzitet u Beogradu - Medicinski fakultet, Beograd., 42(2), 17-22.
https://hdl.handle.net/21.15107/rcub_technorep_1263

Medović A, Kojić Z, Tasić G, Milaković B, Kostić M, Škundrić P. Toksičnost i kinetika otpuštanja insulina iz hormonaktivnih polisaharidnih vlakana. in Medicinska istraživanja. 2008;42(2):17-22.
https://hdl.handle.net/21.15107/rcub_technorep_1263 .

Medović, Adela, Kojić, Zvezdana, Tasić, Goran, Milaković, Branko, Kostić, Mirjana, Škundrić, Petar, "Toksičnost i kinetika otpuštanja insulina iz hormonaktivnih polisaharidnih vlakana" in Medicinska istraživanja, 42, no. 2 (2008):17-22,
https://hdl.handle.net/21.15107/rcub_technorep_1263 .