TY  - JOUR
AU  - Jonović, Marko
AU  - Jugović, Branimir
AU  - Žuža, Milena
AU  - Ðorđević, Verica
AU  - Milašinović, Nikola
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5165
AB  - The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.
PB  - MDPI
T2  - Polymers
T1  - Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation
IS  - 13
SP  - 2614
VL  - 14
DO  - 10.3390/polym14132614
ER  - 

@article{
author = "Jonović, Marko and Jugović, Branimir and Žuža, Milena and Ðorđević, Verica and Milašinović, Nikola and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "The aim of this study was to investigate covalent immobilization of horseradish peroxidase (HRP) on magnetic nanoparticles (Mag) encapsulated in calcium alginate beads (MABs) for color degradation, combining easy and fast removal of biocatalyst from the reaction mixture due to its magnetic properties and strong binding due to surface alginate functional groups. MABs obtained by extrusion techniques were analyzed by optical microscopy, FEG-SEM and characterized regarding mechanical properties, magnetization and HRP binding. HRP with initial concentration of 10 mg/gcarrier was successfully covalently bonded on MABs (diameter ~1 mm, magnetite/alginate ratio 1:4), with protein loading of 8.9 mg/gcarrier, immobilization yield 96.9% and activity 32.8 U/g. Immobilized HRP on MABs (HRP-MABs) was then used to catalyze degradation of two anthraquinonic dyes, Acid Blue 225 (AB225) and Acid Violet 109 (AV109), as models for wastewater pollutants. HRP-MABs decolorized 77.3% and 76.1% of AV109 and AB225, respectively after 15 min under optimal conditions (0.097 mM H2O2, 200 mg of HRP-MABs (8.9 mg/gcarrier), 0.08 and 0.1 g/mg beads/dye ratio for AV109 and AB225, respectively). Biocatalyst was used for 7 repeated cycles retaining 75% and 51% of initial activity for AB225 and AV109, respectively, showing potential for use in large scale applications for colored wastewater treatment.",
publisher = "MDPI",
journal = "Polymers",
title = "Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation",
number = "13",
pages = "2614",
volume = "14",
doi = "10.3390/polym14132614"
}

Jonović, M., Jugović, B., Žuža, M., Ðorđević, V., Milašinović, N., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation. in Polymers
MDPI., 14(13), 2614.
https://doi.org/10.3390/polym14132614

Jonović M, Jugović B, Žuža M, Ðorđević V, Milašinović N, Bugarski B, Knežević-Jugović Z. Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation. in Polymers. 2022;14(13):2614.
doi:10.3390/polym14132614 .

Jonović, Marko, Jugović, Branimir, Žuža, Milena, Ðorđević, Verica, Milašinović, Nikola, Bugarski, Branko, Knežević-Jugović, Zorica, "Immobilization of Horseradish Peroxidase on Magnetite-Alginate Beads to Enable Effective Strong Binding and Enzyme Recycling during Anthraquinone Dyes’ Degradation" in Polymers, 14, no. 13 (2022):2614,
https://doi.org/10.3390/polym14132614 . .

TY  - JOUR
AU  - Vučković, N.
AU  - Glođović, N.
AU  - Radovanović, Željko
AU  - Janaćković, Đorđe
AU  - Milašinović, Nikola
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4569
AB  - Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems, there are very few studies that address this biopolymer utilization in forensic applications, such as the detection of latent fingerprints. We used chitosan crosslinked with sodium tripolyphosphate and conjugated with L-lysine to enhance the visualization of latent fingerprints, due to its ability to interact with fingerprint sweat residues. These conjugates were prepared using different (w/w) amounts of chitosan and tripolyphosphate (6/1; 4/1; 1/1; 1/4; and 1/6), and those with the most promising results (i.e., 6/1 formulation) were investigated in detail. Fourier transform infrared (FT-IR) spectroscopy confirmed interactions between components of the systems. Optical microscopy and scanning electron microscope (SEM) analysis showed that prepared powder formulations were uniform in size and confirmed that chitosan/tripolyphosphate/lysine conjugates bind easily to the sweat and lipid residues present in the latent fingerprints. The testing of prepared conjugates demonstrated the potential of these systems as bio-based powder substitution for commercially available powders.
T2  - Journal of Forensic Sciences
T1  - A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement
DO  - 10.1111/1556-4029.14569
ER  - 

@article{
author = "Vučković, N. and Glođović, N. and Radovanović, Željko and Janaćković, Đorđe and Milašinović, Nikola",
year = "2020",
abstract = "Most chemical and physical methods employed in visualizing latent marks have shown detrimental effects on human health and, therefore, some research groups have directed their attention to the utilization of various (bio)polymers with the aforementioned purpose. Although chitosan is widely used in medicine, pharmacy, food, and drug delivery systems, there are very few studies that address this biopolymer utilization in forensic applications, such as the detection of latent fingerprints. We used chitosan crosslinked with sodium tripolyphosphate and conjugated with L-lysine to enhance the visualization of latent fingerprints, due to its ability to interact with fingerprint sweat residues. These conjugates were prepared using different (w/w) amounts of chitosan and tripolyphosphate (6/1; 4/1; 1/1; 1/4; and 1/6), and those with the most promising results (i.e., 6/1 formulation) were investigated in detail. Fourier transform infrared (FT-IR) spectroscopy confirmed interactions between components of the systems. Optical microscopy and scanning electron microscope (SEM) analysis showed that prepared powder formulations were uniform in size and confirmed that chitosan/tripolyphosphate/lysine conjugates bind easily to the sweat and lipid residues present in the latent fingerprints. The testing of prepared conjugates demonstrated the potential of these systems as bio-based powder substitution for commercially available powders.",
journal = "Journal of Forensic Sciences",
title = "A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement",
doi = "10.1111/1556-4029.14569"
}

Vučković, N., Glođović, N., Radovanović, Ž., Janaćković, Đ.,& Milašinović, N.. (2020). A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement. in Journal of Forensic Sciences.
https://doi.org/10.1111/1556-4029.14569

Vučković N, Glođović N, Radovanović Ž, Janaćković Đ, Milašinović N. A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement. in Journal of Forensic Sciences. 2020;.
doi:10.1111/1556-4029.14569 .

Vučković, N., Glođović, N., Radovanović, Željko, Janaćković, Đorđe, Milašinović, Nikola, "A novel chitosan/tripolyphosphate/L-lysine conjugates for latent fingerprints detection and enhancement" in Journal of Forensic Sciences (2020),
https://doi.org/10.1111/1556-4029.14569 . .

Gligorijević, B. R., Milašinović, V. D., Milašinović, M., Milašinović, N., Jevremović, A., Raić, K., Alil, A., Dikić, S., Vlahović, M.,& Martinović, S.. (2019). Poboljšanje elektro-mehaničkih svojstava Al/Cu električnih konektora smanjenjem intermetalnog sloja postupkom redukovanja vremena zavarivanja trenjem. in Tehnološko-metalurški fakultet
Tehnološko-metalurški fakultet., 1-13.
https://hdl.handle.net/21.15107/rcub_technorep_6236

Gligorijević BR, Milašinović VD, Milašinović M, Milašinović N, Jevremović A, Raić K, Alil A, Dikić S, Vlahović M, Martinović S. Poboljšanje elektro-mehaničkih svojstava Al/Cu električnih konektora smanjenjem intermetalnog sloja postupkom redukovanja vremena zavarivanja trenjem. in Tehnološko-metalurški fakultet. 2019;:1-13.
https://hdl.handle.net/21.15107/rcub_technorep_6236 .

Gligorijević, Bojan R., Milašinović, Veljko D., Milašinović, Mijat, Milašinović, Nikola, Jevremović, Aleksandar, Raić, Karlo, Alil, Ana, Dikić, Stefan, Vlahović, Milica, Martinović, Sanja, "Poboljšanje elektro-mehaničkih svojstava Al/Cu električnih konektora smanjenjem intermetalnog sloja postupkom redukovanja vremena zavarivanja trenjem" in Tehnološko-metalurški fakultet (2019):1-13,
https://hdl.handle.net/21.15107/rcub_technorep_6236 .

TY  - JOUR
AU  - Terzić, Ivan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
AU  - Lučić-Škorić, Marija
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Kalagasidis Krušić, Melina
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3946
AB  - Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018.
PB  - Wiley, Hoboken
T2  - Polymer Engineering and Science
T1  - A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol
EP  - 2199
IS  - 12
SP  - 2192
VL  - 58
DO  - 10.1002/pen.24834
ER  - 

@article{
author = "Terzić, Ivan and Ivanović, Jasna and Žižović, Irena and Lučić-Škorić, Marija and Milosavljević, Nedeljko and Milašinović, Nikola and Kalagasidis Krušić, Melina",
year = "2018",
abstract = "Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018.",
publisher = "Wiley, Hoboken",
journal = "Polymer Engineering and Science",
title = "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol",
pages = "2199-2192",
number = "12",
volume = "58",
doi = "10.1002/pen.24834"
}

Terzić, I., Ivanović, J., Žižović, I., Lučić-Škorić, M., Milosavljević, N., Milašinović, N.,& Kalagasidis Krušić, M.. (2018). A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer Engineering and Science
Wiley, Hoboken., 58(12), 2192-2199.
https://doi.org/10.1002/pen.24834

Terzić I, Ivanović J, Žižović I, Lučić-Škorić M, Milosavljević N, Milašinović N, Kalagasidis Krušić M. A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer Engineering and Science. 2018;58(12):2192-2199.
doi:10.1002/pen.24834 .

Terzić, Ivan, Ivanović, Jasna, Žižović, Irena, Lučić-Škorić, Marija, Milosavljević, Nedeljko, Milašinović, Nikola, Kalagasidis Krušić, Melina, "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol" in Polymer Engineering and Science, 58, no. 12 (2018):2192-2199,
https://doi.org/10.1002/pen.24834 . .

TY  - JOUR
AU  - Babić, Filip
AU  - Balanč, Bojana
AU  - Milašinović, Nikola
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3798
AB  - The role of the materials used for packaging is to protect inner content from external, mostly negative, factors. Besides being safe, and with good mechanical properties, these materials need to align with other consumers’ needs and demands, being both attractive and authentic, while at the same time meeting ecological requirements. Gelatin-based thin films are good candidates for production of semi-soft cup-shaped products that could be used for cruciferous vegetables packaging. These films were prepared by a simple solvent evaporation method. Prepared films were characterized by swelling study and their mechanical properties were investigated. The optical characteristics of the films were determined by optical microscopy revealing that these systems possess attractive clear and transparent gold-yellowish appearance with a smooth surface.

Keywords: Gelatin, Glycerol, Chamomile Tea Extract, Polymer Thin Films
PB  - University of East Sarajevo, Faculty of Technology
T2  - Journal of Engineering & Processing Management
T1  - Reducing packaging waste – mechanical characteristics and network parameters of the gelatin-based thin film for cruciferous vegetables packaging
EP  - 27
IS  - 1
SP  - 21
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3798
ER  - 

@article{
author = "Babić, Filip and Balanč, Bojana and Milašinović, Nikola",
year = "2018",
abstract = "The role of the materials used for packaging is to protect inner content from external, mostly negative, factors. Besides being safe, and with good mechanical properties, these materials need to align with other consumers’ needs and demands, being both attractive and authentic, while at the same time meeting ecological requirements. Gelatin-based thin films are good candidates for production of semi-soft cup-shaped products that could be used for cruciferous vegetables packaging. These films were prepared by a simple solvent evaporation method. Prepared films were characterized by swelling study and their mechanical properties were investigated. The optical characteristics of the films were determined by optical microscopy revealing that these systems possess attractive clear and transparent gold-yellowish appearance with a smooth surface.

Keywords: Gelatin, Glycerol, Chamomile Tea Extract, Polymer Thin Films",
publisher = "University of East Sarajevo, Faculty of Technology",
journal = "Journal of Engineering & Processing Management",
title = "Reducing packaging waste – mechanical characteristics and network parameters of the gelatin-based thin film for cruciferous vegetables packaging",
pages = "27-21",
number = "1",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3798"
}

Babić, F., Balanč, B.,& Milašinović, N.. (2018). Reducing packaging waste – mechanical characteristics and network parameters of the gelatin-based thin film for cruciferous vegetables packaging. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology., 10(1), 21-27.
https://hdl.handle.net/21.15107/rcub_technorep_3798

Babić F, Balanč B, Milašinović N. Reducing packaging waste – mechanical characteristics and network parameters of the gelatin-based thin film for cruciferous vegetables packaging. in Journal of Engineering & Processing Management. 2018;10(1):21-27.
https://hdl.handle.net/21.15107/rcub_technorep_3798 .

Babić, Filip, Balanč, Bojana, Milašinović, Nikola, "Reducing packaging waste – mechanical characteristics and network parameters of the gelatin-based thin film for cruciferous vegetables packaging" in Journal of Engineering & Processing Management, 10, no. 1 (2018):21-27,
https://hdl.handle.net/21.15107/rcub_technorep_3798 .

TY  - JOUR
AU  - Žuža, Milena
AU  - Milašinović, Nikola
AU  - Jonović, Marko M.
AU  - Jovanović, Jelena
AU  - Kalagasidis Krušić, Melina
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3544
AB  - In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Design and characterization of alcalase-chitosan conjugates as potential biocatalysts
EP  - 1723
IS  - 11
SP  - 1713
VL  - 40
DO  - 10.1007/s00449-017-1826-7
ER  - 

@article{
author = "Žuža, Milena and Milašinović, Nikola and Jonović, Marko M. and Jovanović, Jelena and Kalagasidis Krušić, Melina and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2017",
abstract = "In this study, alcalase (protease from Bacillus licheniformis) immobilization by adsorption, enzyme crosslinking and covalent enzyme binding to activated chitosan microbeads were examined. The biocatalysts highest activity was obtained by covalent immobilization of alcalase onto a solid support. The alcalase covalent immobilization onto different types of chitosan beads obtained by inverse emulsion technique and electrostatic extrusion was studied. Parameters examined under different conditions were beads diameter, enzyme loading, enzyme capacity yield, and biocatalyst activity. The highest activity and enzyme loading of 23.6 IU/mg protein and 340.2 mg/g, respectively, were achieved by the enzyme immobilized onto chitosan microbeads obtained by the electrostatic extrusion technique. FT-IR analysis was used to confirm formation of alcalase-chitosan conjugates. The activity of optimally produced alcalase-chitosan microbeads was then verified in the industrially feasible reaction systems of egg white and soy protein hydrolysis. The high degree of hydrolysis of 29.85 +/- 0.967% after 180 min and five successive reuses obtained under real conditions (50 A degrees C, pH 8) verified the covalently bound alcalase to chitosan beads a promising candidate for use in industrial egg white protein hydrolysis process.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts",
pages = "1723-1713",
number = "11",
volume = "40",
doi = "10.1007/s00449-017-1826-7"
}

Žuža, M., Milašinović, N., Jonović, M. M., Jovanović, J., Kalagasidis Krušić, M., Bugarski, B.,& Knežević-Jugović, Z.. (2017). Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and Biosystems Engineering
Springer, New York., 40(11), 1713-1723.
https://doi.org/10.1007/s00449-017-1826-7

Žuža M, Milašinović N, Jonović MM, Jovanović J, Kalagasidis Krušić M, Bugarski B, Knežević-Jugović Z. Design and characterization of alcalase-chitosan conjugates as potential biocatalysts. in Bioprocess and Biosystems Engineering. 2017;40(11):1713-1723.
doi:10.1007/s00449-017-1826-7 .

Žuža, Milena, Milašinović, Nikola, Jonović, Marko M., Jovanović, Jelena, Kalagasidis Krušić, Melina, Bugarski, Branko, Knežević-Jugović, Zorica, "Design and characterization of alcalase-chitosan conjugates as potential biocatalysts" in Bioprocess and Biosystems Engineering, 40, no. 11 (2017):1713-1723,
https://doi.org/10.1007/s00449-017-1826-7 . .

TY  - CHAP
AU  - Lević, Steva
AU  - Ðorđević, Verica
AU  - Knežević-Jugović, Zorica
AU  - Kalušević, Ana
AU  - Milašinović, Nikola
AU  - Bugarski, Branko M.
AU  - Nedović, Viktor A.
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6180
AB  - The application of immobilized enzyme is strictly related to economic benefits of immobilization. Regardless of numerous patents and published articles, application of immobilized enzymes in food industry is relatively limited to several processes. Immobilization of enzyme onto inorganic carriers is a well-established technique, especially in the field of industrial applications. Zeolites are another group of inorganic materials that have been applied in enzyme immobilization. Cysteine was also found to be suitable as a binding agent for enzyme immobilization on to the glass surface. The covalent immobilization of enzymes onto epoxy-activated carriers has drawn considerable interest. A new process for synthesis of carriers, such as electrochemical synthesis of polyaniline, was found to be a promising procedure for production of stable enzyme support. Natural and synthetic polymer hydrogels have been frequently used for encapsulation of cells, enzymes and food compounds.
PB  - CRC Press
T2  - Microbial Enzyme Technology in Food Applications
T1  - Enzyme Encapsulation Technologies and their Applications in Food Processing
EP  - 502
SP  - 469
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6180
ER  - 

@inbook{
author = "Lević, Steva and Ðorđević, Verica and Knežević-Jugović, Zorica and Kalušević, Ana and Milašinović, Nikola and Bugarski, Branko M. and Nedović, Viktor A.",
year = "2016",
abstract = "The application of immobilized enzyme is strictly related to economic benefits of immobilization. Regardless of numerous patents and published articles, application of immobilized enzymes in food industry is relatively limited to several processes. Immobilization of enzyme onto inorganic carriers is a well-established technique, especially in the field of industrial applications. Zeolites are another group of inorganic materials that have been applied in enzyme immobilization. Cysteine was also found to be suitable as a binding agent for enzyme immobilization on to the glass surface. The covalent immobilization of enzymes onto epoxy-activated carriers has drawn considerable interest. A new process for synthesis of carriers, such as electrochemical synthesis of polyaniline, was found to be a promising procedure for production of stable enzyme support. Natural and synthetic polymer hydrogels have been frequently used for encapsulation of cells, enzymes and food compounds.",
publisher = "CRC Press",
journal = "Microbial Enzyme Technology in Food Applications",
booktitle = "Enzyme Encapsulation Technologies and their Applications in Food Processing",
pages = "502-469",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6180"
}

Lević, S., Ðorđević, V., Knežević-Jugović, Z., Kalušević, A., Milašinović, N., Bugarski, B. M.,& Nedović, V. A.. (2016). Enzyme Encapsulation Technologies and their Applications in Food Processing. in Microbial Enzyme Technology in Food Applications
CRC Press., 469-502.
https://hdl.handle.net/21.15107/rcub_technorep_6180

Lević S, Ðorđević V, Knežević-Jugović Z, Kalušević A, Milašinović N, Bugarski BM, Nedović VA. Enzyme Encapsulation Technologies and their Applications in Food Processing. in Microbial Enzyme Technology in Food Applications. 2016;:469-502.
https://hdl.handle.net/21.15107/rcub_technorep_6180 .

Lević, Steva, Ðorđević, Verica, Knežević-Jugović, Zorica, Kalušević, Ana, Milašinović, Nikola, Bugarski, Branko M., Nedović, Viktor A., "Enzyme Encapsulation Technologies and their Applications in Food Processing" in Microbial Enzyme Technology in Food Applications (2016):469-502,
https://hdl.handle.net/21.15107/rcub_technorep_6180 .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Kotur-Stevuljević, Jelena
AU  - Dilber, Sanda P.
AU  - Kalagasidis Krušić, Melina
AU  - Đoržević, Brižita
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3469
AB  - Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity
EP  - 58
IS  - 1
SP  - 49
VL  - 70
DO  - 10.2298/HEMIND141119010V
ER  - 

@article{
author = "Vidović, Bojana and Milašinović, Nikola and Kotur-Stevuljević, Jelena and Dilber, Sanda P. and Kalagasidis Krušić, Melina and Đoržević, Brižita and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity",
pages = "58-49",
number = "1",
volume = "70",
doi = "10.2298/HEMIND141119010V"
}

Vidović, B., Milašinović, N., Kotur-Stevuljević, J., Dilber, S. P., Kalagasidis Krušić, M., Đoržević, B.,& Knežević-Jugović, Z.. (2016). Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(1), 49-58.
https://doi.org/10.2298/HEMIND141119010V

Vidović B, Milašinović N, Kotur-Stevuljević J, Dilber SP, Kalagasidis Krušić M, Đoržević B, Knežević-Jugović Z. Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija. 2016;70(1):49-58.
doi:10.2298/HEMIND141119010V .

Vidović, Bojana, Milašinović, Nikola, Kotur-Stevuljević, Jelena, Dilber, Sanda P., Kalagasidis Krušić, Melina, Đoržević, Brižita, Knežević-Jugović, Zorica, "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity" in Hemijska industrija, 70, no. 1 (2016):49-58,
https://doi.org/10.2298/HEMIND141119010V . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Čalija, Bojan
AU  - Vidović, Bojana
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3456
AB  - The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of the Taiwan Institute of Chemical Engineers
T1  - Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
EP  - 112
SP  - 106
VL  - 60
DO  - 10.1016/j.jtice.2015.10.037
ER  - 

@article{
author = "Milašinović, Nikola and Čalija, Bojan and Vidović, Bojana and Crevar-Sakač, Milkica and Vujić, Zorica and Knežević-Jugović, Zorica",
year = "2016",
abstract = "The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
title = "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method",
pages = "112-106",
volume = "60",
doi = "10.1016/j.jtice.2015.10.037"
}

Milašinović, N., Čalija, B., Vidović, B., Crevar-Sakač, M., Vujić, Z.,& Knežević-Jugović, Z.. (2016). Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers
Elsevier Science Bv, Amsterdam., 60, 106-112.
https://doi.org/10.1016/j.jtice.2015.10.037

Milašinović N, Čalija B, Vidović B, Crevar-Sakač M, Vujić Z, Knežević-Jugović Z. Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers. 2016;60:106-112.
doi:10.1016/j.jtice.2015.10.037 .

Milašinović, Nikola, Čalija, Bojan, Vidović, Bojana, Crevar-Sakač, Milkica, Vujić, Zorica, Knežević-Jugović, Zorica, "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method" in Journal of the Taiwan Institute of Chemical Engineers, 60 (2016):106-112,
https://doi.org/10.1016/j.jtice.2015.10.037 . .

TY  - CONF
AU  - Trifković, Kata
AU  - Isailović, Bojana
AU  - Milašinović, Nikola
AU  - Jovanović, Aleksandra
AU  - Knežević-Jugović, Zorica
AU  - Đorđević, Verica
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6453
AB  - In this work mechanical behavior of four different formulations of alginate-based beads was
tested. The alginate beads were prepared by means of extrusion technique. As a model
encapsulated bioactive substance resveratrol-loaded liposomes were used. The beads were
composed as follows: 1.5% alginate beads; 1.5% alginate beads encapsulating liposomes; 1.5%
alginate/30% sucrose beads encapsulating liposomes and 1.5% alginate/0.5% chitosan beads
encapsulating liposomes. The mechanical properties (e.g. maximal forces required for reaching
the default deformation, maximal stresses and the Young’s modulus) of aforementioned beads
were determined by testing the single bead submerged in water using the AG-X plus Universal
Testing Machine (Shimadzu, Japan). Results showed the decrease in maximal forces (stresses)
and increase in elasticity of the examined beads upon encapsulation of resveratrol-loaded
liposomes. After addition of sucrose and coating with chitosan, maximal forces respectively
remained constant and decreased, while elastic modulus also remained constant and increased,
respectively. Tested compression method can be used in the determination of mechanical
properties of different alginate-based beads.
AB  - U ovom radu su ispitane mehaničke karakteristike četiri različite formulacije čestica na bazi
alginata. Čestice su pripremljenje koristeći metodu ekstruzije. Kao model supstanca za
inkapsulaciju korišćni su lipozomi sa inkapsuliranim resveratrolom. Formulacije čestica su
sledeće: 1,5% alginatne čestice; 1,5% alginatne čestice sa inkapsuliranim lipozomima; 1,5%
alginatne čestice sa inkapsuliranim lipozomima sa dodatkom 30% saharoze i 1,5% alginatne
čestice sa inkapsuliranim lipozomima sa dodatnim slojem 0,5% hitozana. Mehaničke
karakteristike (tj. maksimalne sile potrebne za dostizanje unapred definisane deformacije,
maksimalni naponi i Jungovi moduli elastičnosti) gore pomenutih čestica su određene
testiranjem pojedinačnih čestica uronjenih u vodu, koristeći AG-X plus Universal Testing
Machine (Shimadzu, Japan). Rezultati su pokazali da nakon inkapsulacije lipozoma sa
resveratrolom u alginatne čestice dolazi do smanjenja maksimalne sile potrebne za postizanje
zadate deformacije, kao i do povećanja elastičnosti ispitivanh čestica. Nakon dodatka saharoze i
oblaganja alginatnih čestica slojem hitozana, maksimalna sila ostaje nepromenjana, odnosno
dolazi do njenog smanjenja; slicno, vrednosti modula elastičnosti takođe ostaju konstantne,
odnosno povećavaju se. Ispitivana metoda kompresije može se koristiti za određivanje
mehaničkih karakteristika različitih čestica na bazi alginata.
PB  - Faculty of Technology, Zvornik
C3  - IV International Congress of “Engineering, Environment and Materials in Processing Industry“
T1  - Mechanical properties of alginate-liposomes-based beads with encapsulated resveratrol
T1  - Mehaničke karakteristike čestica alginat-lipozomi sa inkapsuliranim resveratrolom
EP  - 325
SP  - 318
DO  - 10.7251/EEMEN1501318T
ER  - 

@conference{
author = "Trifković, Kata and Isailović, Bojana and Milašinović, Nikola and Jovanović, Aleksandra and Knežević-Jugović, Zorica and Đorđević, Verica and Bugarski, Branko",
year = "2015",
abstract = "In this work mechanical behavior of four different formulations of alginate-based beads was
tested. The alginate beads were prepared by means of extrusion technique. As a model
encapsulated bioactive substance resveratrol-loaded liposomes were used. The beads were
composed as follows: 1.5% alginate beads; 1.5% alginate beads encapsulating liposomes; 1.5%
alginate/30% sucrose beads encapsulating liposomes and 1.5% alginate/0.5% chitosan beads
encapsulating liposomes. The mechanical properties (e.g. maximal forces required for reaching
the default deformation, maximal stresses and the Young’s modulus) of aforementioned beads
were determined by testing the single bead submerged in water using the AG-X plus Universal
Testing Machine (Shimadzu, Japan). Results showed the decrease in maximal forces (stresses)
and increase in elasticity of the examined beads upon encapsulation of resveratrol-loaded
liposomes. After addition of sucrose and coating with chitosan, maximal forces respectively
remained constant and decreased, while elastic modulus also remained constant and increased,
respectively. Tested compression method can be used in the determination of mechanical
properties of different alginate-based beads., U ovom radu su ispitane mehaničke karakteristike četiri različite formulacije čestica na bazi
alginata. Čestice su pripremljenje koristeći metodu ekstruzije. Kao model supstanca za
inkapsulaciju korišćni su lipozomi sa inkapsuliranim resveratrolom. Formulacije čestica su
sledeće: 1,5% alginatne čestice; 1,5% alginatne čestice sa inkapsuliranim lipozomima; 1,5%
alginatne čestice sa inkapsuliranim lipozomima sa dodatkom 30% saharoze i 1,5% alginatne
čestice sa inkapsuliranim lipozomima sa dodatnim slojem 0,5% hitozana. Mehaničke
karakteristike (tj. maksimalne sile potrebne za dostizanje unapred definisane deformacije,
maksimalni naponi i Jungovi moduli elastičnosti) gore pomenutih čestica su određene
testiranjem pojedinačnih čestica uronjenih u vodu, koristeći AG-X plus Universal Testing
Machine (Shimadzu, Japan). Rezultati su pokazali da nakon inkapsulacije lipozoma sa
resveratrolom u alginatne čestice dolazi do smanjenja maksimalne sile potrebne za postizanje
zadate deformacije, kao i do povećanja elastičnosti ispitivanh čestica. Nakon dodatka saharoze i
oblaganja alginatnih čestica slojem hitozana, maksimalna sila ostaje nepromenjana, odnosno
dolazi do njenog smanjenja; slicno, vrednosti modula elastičnosti takođe ostaju konstantne,
odnosno povećavaju se. Ispitivana metoda kompresije može se koristiti za određivanje
mehaničkih karakteristika različitih čestica na bazi alginata.",
publisher = "Faculty of Technology, Zvornik",
journal = "IV International Congress of “Engineering, Environment and Materials in Processing Industry“",
title = "Mechanical properties of alginate-liposomes-based beads with encapsulated resveratrol, Mehaničke karakteristike čestica alginat-lipozomi sa inkapsuliranim resveratrolom",
pages = "325-318",
doi = "10.7251/EEMEN1501318T"
}

Trifković, K., Isailović, B., Milašinović, N., Jovanović, A., Knežević-Jugović, Z., Đorđević, V.,& Bugarski, B.. (2015). Mechanical properties of alginate-liposomes-based beads with encapsulated resveratrol. in IV International Congress of “Engineering, Environment and Materials in Processing Industry“
Faculty of Technology, Zvornik., 318-325.
https://doi.org/10.7251/EEMEN1501318T

Trifković K, Isailović B, Milašinović N, Jovanović A, Knežević-Jugović Z, Đorđević V, Bugarski B. Mechanical properties of alginate-liposomes-based beads with encapsulated resveratrol. in IV International Congress of “Engineering, Environment and Materials in Processing Industry“. 2015;:318-325.
doi:10.7251/EEMEN1501318T .

Trifković, Kata, Isailović, Bojana, Milašinović, Nikola, Jovanović, Aleksandra, Knežević-Jugović, Zorica, Đorđević, Verica, Bugarski, Branko, "Mechanical properties of alginate-liposomes-based beads with encapsulated resveratrol" in IV International Congress of “Engineering, Environment and Materials in Processing Industry“ (2015):318-325,
https://doi.org/10.7251/EEMEN1501318T . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Kalagasidis Krušić, Melina
AU  - Knežević-Jugović, Zorica
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3077
AB  - Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.
PB  - Sage Publications Ltd, London
T2  - Journal of Biomaterials Applications
T1  - Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties
EP  - 631
IS  - 5
SP  - 618
VL  - 30
DO  - 10.1177/0885328215598940
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica and Zdunić, Gordana and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko",
year = "2015",
abstract = "Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Biomaterials Applications",
title = "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties",
pages = "631-618",
number = "5",
volume = "30",
doi = "10.1177/0885328215598940"
}

Trifković, K. T., Milašinović, N., Đorđević, V., Zdunić, G., Kalagasidis Krušić, M., Knežević-Jugović, Z., Šavikin, K., Nedović, V.,& Bugarski, B.. (2015). Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of Biomaterials Applications
Sage Publications Ltd, London., 30(5), 618-631.
https://doi.org/10.1177/0885328215598940

Trifković KT, Milašinović N, Đorđević V, Zdunić G, Kalagasidis Krušić M, Knežević-Jugović Z, Šavikin K, Nedović V, Bugarski B. Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of Biomaterials Applications. 2015;30(5):618-631.
doi:10.1177/0885328215598940 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica, Zdunić, Gordana, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties" in Journal of Biomaterials Applications, 30, no. 5 (2015):618-631,
https://doi.org/10.1177/0885328215598940 . .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Vidović, Jana
AU  - Čalija, Bojan
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Milić, Jela R.
AU  - Đoržević, Brižita
AU  - Kalagasidis Krušić, Melina
AU  - Knežević-Jugović, Zorica
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3078
PB  - Karger, Basel
T2  - Annals of Nutrition and Metabolism
T1  - Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity
EP  - 485
SP  - 484
VL  - 67
UR  - https://hdl.handle.net/21.15107/rcub_technorep_3078
ER  - 

@article{
author = "Vidović, Bojana and Milašinović, Nikola and Vidović, Jana and Čalija, Bojan and Crevar-Sakač, Milkica and Vujić, Zorica and Milić, Jela R. and Đoržević, Brižita and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica",
year = "2015",
publisher = "Karger, Basel",
journal = "Annals of Nutrition and Metabolism",
title = "Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity",
pages = "485-484",
volume = "67",
url = "https://hdl.handle.net/21.15107/rcub_technorep_3078"
}

Vidović, B., Milašinović, N., Vidović, J., Čalija, B., Crevar-Sakač, M., Vujić, Z., Milić, J. R., Đoržević, B., Kalagasidis Krušić, M.,& Knežević-Jugović, Z.. (2015). Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity. in Annals of Nutrition and Metabolism
Karger, Basel., 67, 484-485.
https://hdl.handle.net/21.15107/rcub_technorep_3078

Vidović B, Milašinović N, Vidović J, Čalija B, Crevar-Sakač M, Vujić Z, Milić JR, Đoržević B, Kalagasidis Krušić M, Knežević-Jugović Z. Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity. in Annals of Nutrition and Metabolism. 2015;67:484-485.
https://hdl.handle.net/21.15107/rcub_technorep_3078 .

Vidović, Bojana, Milašinović, Nikola, Vidović, Jana, Čalija, Bojan, Crevar-Sakač, Milkica, Vujić, Zorica, Milić, Jela R., Đoržević, Brižita, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, "Preparation of alpha-lipoic acid/chitosan microparticle conjugate and its in vitro antioxidative activity" in Annals of Nutrition and Metabolism, 67 (2015):484-485,
https://hdl.handle.net/21.15107/rcub_technorep_3078 .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Jakovetić, Sonja
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lučić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2771
AB  - This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Hindawi Publishing Corp, New York
T2  - Scientific World Journal
T1  - Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
DO  - 10.1155/2014/142123
ER  - 

@article{
author = "Milašinović, Nikola and Jakovetić, Sonja and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lučić, Marija and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2014",
abstract = "This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to be 40 and 45 degrees C, while the optimum pH was observed at pH 7 and 8, respectively. Both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium have been evaluated. Maximum reaction rate (V-max) and Michaelis-Menten constants (K-m) were also determined for immobilized lipase. Storage stability of lipase was increased as a result of immobilization process. Furthermore, the operational stability and reusability of the immobilized lipase in esterification reaction have been studied, and it was observed that after 10 cycles, the residual activity for entrapped lipase was as high as 50%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Hindawi Publishing Corp, New York",
journal = "Scientific World Journal",
title = "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel",
doi = "10.1155/2014/142123"
}

Milašinović, N., Jakovetić, S., Knežević-Jugović, Z., Milosavljević, N., Lučić, M., Filipović, J.,& Kalagasidis Krušić, M.. (2014). Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal
Hindawi Publishing Corp, New York..
https://doi.org/10.1155/2014/142123

Milašinović N, Jakovetić S, Knežević-Jugović Z, Milosavljević N, Lučić M, Filipović J, Kalagasidis Krušić M. Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel. in Scientific World Journal. 2014;.
doi:10.1155/2014/142123 .

Milašinović, Nikola, Jakovetić, Sonja, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel" in Scientific World Journal (2014),
https://doi.org/10.1155/2014/142123 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Lučić-Škorić, Marija
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2828
AB  - Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
VL  - 2014
DO  - 10.1155/2014/364930
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Lučić-Škorić, Marija and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2014",
abstract = "Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic environment of upper gastrointestinal tract and deliver it in the neutral or slightly basic region of the lower intestine. The protein/poly(NiPAAm-co-IA) hydrogels were synthetized by free radical crosslinking copolymerization and were characterized concerning their swelling capability, mechanical properties, and morphology. The pore structure and sizes up to 1.90 nm allowed good entrapment of lipase molecules. Model protein, lipase from Candida rugosa, was entrapped within hydrogels upon mild conditions that provided its protection from harmful environmental influences. The efficiency of the lipase entrapment reached 96.7%, and was dependent on the initial concentration of lipase solution. The swelling of the obtained hydrogels in simulated pH and temperature of gastrointestinal tract, the lipase entrapment efficiency, and its release profiles from hydrogels were investigated as well.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions",
volume = "2014",
doi = "10.1155/2014/364930"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Lučić-Škorić, M., Filipović, J.,& Kalagasidis Krušić, M.. (2014). Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed Research International
Hindawi Ltd, London., 2014.
https://doi.org/10.1155/2014/364930

Milašinović N, Knežević-Jugović Z, Milosavljević N, Lučić-Škorić M, Filipović J, Kalagasidis Krušić M. Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions. in Biomed Research International. 2014;2014.
doi:10.1155/2014/364930 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Lučić-Škorić, Marija, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions" in Biomed Research International, 2014 (2014),
https://doi.org/10.1155/2014/364930 . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica
AU  - Kalagasidis Krušić, Melina
AU  - Knežević-Jugović, Zorica
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2647
AB  - In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. The obtained microgel beads from similar to 220 to similar to 790 mu m in diameter were exposed to controlled drying process at air (at 37 degrees C) after which they contracted to irregular shapes (similar to 70-230 mu m). The loading of dried microbeads with polyphenols was achieved by swelling in the acidic medium. The swelling rate of microbeads decreased with the increase in GA concentration. Upon this rehydration, thyme polypheriols were effectively encapsulated (active load of 66-114mg GAEg(beads)(-1)) and the microbeads recovered a spherical shape. Both, the increase in the amount of the crosslinking agent and the presence of polyphenols, contributed to a more pronounced surface roughness of microbeads. The release of encapsulated polyphenols in simulated gastrointestinal fluids was prolonged to 3 h.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols
EP  - 907
SP  - 901
VL  - 111
DO  - 10.1016/j.carbpol.2014.05.053
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica and Nedović, Viktor and Bugarski, Branko",
year = "2014",
abstract = "In this work chitosan microbeads were prepared by emulsion technique and loaded with thyme polyphenols by diffusion from an external aqueous solution of Thymus serpyllum L. The effects of concentrations of chitosan (1.5-3% (w/v)) and GA (glutaraldehyde) (0.1-0.4% (v/v)), as a crosslinking agent on the main properties of microbeads were assessed. The obtained microgel beads from similar to 220 to similar to 790 mu m in diameter were exposed to controlled drying process at air (at 37 degrees C) after which they contracted to irregular shapes (similar to 70-230 mu m). The loading of dried microbeads with polyphenols was achieved by swelling in the acidic medium. The swelling rate of microbeads decreased with the increase in GA concentration. Upon this rehydration, thyme polypheriols were effectively encapsulated (active load of 66-114mg GAEg(beads)(-1)) and the microbeads recovered a spherical shape. Both, the increase in the amount of the crosslinking agent and the presence of polyphenols, contributed to a more pronounced surface roughness of microbeads. The release of encapsulated polyphenols in simulated gastrointestinal fluids was prolonged to 3 h.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols",
pages = "907-901",
volume = "111",
doi = "10.1016/j.carbpol.2014.05.053"
}

Trifković, K. T., Milašinović, N., Đorđević, V., Kalagasidis Krušić, M., Knežević-Jugović, Z., Nedović, V.,& Bugarski, B.. (2014). Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 111, 901-907.
https://doi.org/10.1016/j.carbpol.2014.05.053

Trifković KT, Milašinović N, Đorđević V, Kalagasidis Krušić M, Knežević-Jugović Z, Nedović V, Bugarski B. Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. in Carbohydrate Polymers. 2014;111:901-907.
doi:10.1016/j.carbpol.2014.05.053 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, Nedović, Viktor, Bugarski, Branko, "Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols" in Carbohydrate Polymers, 111 (2014):901-907,
https://doi.org/10.1016/j.carbpol.2014.05.053 . .

TY  - JOUR
AU  - Milosavljević, Nedeljko
AU  - Debeljković, Aleksandra
AU  - Kalagasidis Krušić, Melina
AU  - Milašinović, Nikola
AU  - Uzum, O. B.
AU  - Karadag, E.
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2686
AB  - The application of poly(acrylamide-co-sodium methacrylate) hydrogel for the removal of Cu2+ and Cd2+ ions from aqueous solutions has been investigated as a function of pH, adsorbent dose and temperature. FTIR spectra showed that -NH2 and -COOH groups are involved in both metal ions adsorption which followed pseudo-first order kinetics. Nonlinear regression analysis of six isotherms, Langmuir, Freundlich, Redlich-Peterson, Toth, Dubinin-Radushkevich and Sips, have been applied to the sorption data, while the best interpretation was given by Redlich-Peterson. Based on the separation factor, RL, the Cu2+ and Cd2+ ions adsorption is favorable. The maximum adsorption of 24.05 mg/g (48%) for Cu2+ ions and 32.99 mg/g (66%) for Cd2+ ions has been obtained at pH 5.0 and sorbent dosage of 300 mg.
PB  - Wiley, Hoboken
T2  - Environmental Progress & Sustainable Energy
T1  - Application of Poly(acrlymide-co-sodium methacrylate) Hydrogels in Copper and Cadmium Removal from Aqueous Solution
EP  - 834
IS  - 3
SP  - 824
VL  - 33
DO  - 10.1002/ep.11854
ER  - 

@article{
author = "Milosavljević, Nedeljko and Debeljković, Aleksandra and Kalagasidis Krušić, Melina and Milašinović, Nikola and Uzum, O. B. and Karadag, E.",
year = "2014",
abstract = "The application of poly(acrylamide-co-sodium methacrylate) hydrogel for the removal of Cu2+ and Cd2+ ions from aqueous solutions has been investigated as a function of pH, adsorbent dose and temperature. FTIR spectra showed that -NH2 and -COOH groups are involved in both metal ions adsorption which followed pseudo-first order kinetics. Nonlinear regression analysis of six isotherms, Langmuir, Freundlich, Redlich-Peterson, Toth, Dubinin-Radushkevich and Sips, have been applied to the sorption data, while the best interpretation was given by Redlich-Peterson. Based on the separation factor, RL, the Cu2+ and Cd2+ ions adsorption is favorable. The maximum adsorption of 24.05 mg/g (48%) for Cu2+ ions and 32.99 mg/g (66%) for Cd2+ ions has been obtained at pH 5.0 and sorbent dosage of 300 mg.",
publisher = "Wiley, Hoboken",
journal = "Environmental Progress & Sustainable Energy",
title = "Application of Poly(acrlymide-co-sodium methacrylate) Hydrogels in Copper and Cadmium Removal from Aqueous Solution",
pages = "834-824",
number = "3",
volume = "33",
doi = "10.1002/ep.11854"
}

Milosavljević, N., Debeljković, A., Kalagasidis Krušić, M., Milašinović, N., Uzum, O. B.,& Karadag, E.. (2014). Application of Poly(acrlymide-co-sodium methacrylate) Hydrogels in Copper and Cadmium Removal from Aqueous Solution. in Environmental Progress & Sustainable Energy
Wiley, Hoboken., 33(3), 824-834.
https://doi.org/10.1002/ep.11854

Milosavljević N, Debeljković A, Kalagasidis Krušić M, Milašinović N, Uzum OB, Karadag E. Application of Poly(acrlymide-co-sodium methacrylate) Hydrogels in Copper and Cadmium Removal from Aqueous Solution. in Environmental Progress & Sustainable Energy. 2014;33(3):824-834.
doi:10.1002/ep.11854 .

Milosavljević, Nedeljko, Debeljković, Aleksandra, Kalagasidis Krušić, Melina, Milašinović, Nikola, Uzum, O. B., Karadag, E., "Application of Poly(acrlymide-co-sodium methacrylate) Hydrogels in Copper and Cadmium Removal from Aqueous Solution" in Environmental Progress & Sustainable Energy, 33, no. 3 (2014):824-834,
https://doi.org/10.1002/ep.11854 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Jakovljević, Živana
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2235
AB  - A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester  gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Elsevier Science Sa, Lausanne
T2  - Chemical Engineering Journal
T1  - Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels
EP  - 623
SP  - 614
VL  - 181
DO  - 10.1016/j.cej.2011.11.115
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Jakovljević, Živana and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester  gt 90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Chemical Engineering Journal",
title = "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels",
pages = "623-614",
volume = "181",
doi = "10.1016/j.cej.2011.11.115"
}

Milašinović, N., Knežević-Jugović, Z., Jakovljević, Ž., Filipović, J.,& Kalagasidis Krušić, M.. (2012). Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal
Elsevier Science Sa, Lausanne., 181, 614-623.
https://doi.org/10.1016/j.cej.2011.11.115

Milašinović N, Knežević-Jugović Z, Jakovljević Ž, Filipović J, Kalagasidis Krušić M. Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal. 2012;181:614-623.
doi:10.1016/j.cej.2011.11.115 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Jakovljević, Živana, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels" in Chemical Engineering Journal, 181 (2012):614-623,
https://doi.org/10.1016/j.cej.2011.11.115 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Milosavljević, Nedeljko
AU  - Filipović, Jovanka
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2170
AB  - The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid
EP  - 340
IS  - 1-2
SP  - 332
VL  - 436
DO  - 10.1016/j.ijpharm.2012.06.050
ER  - 

@article{
author = "Milašinović, Nikola and Knežević-Jugović, Zorica and Milosavljević, Nedeljko and Filipović, Jovanka and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "The series of poly(N-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from Candida rugosa as a model protein, were synthesized by free radical copolymerization. The composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. All samples were characterized regarding morphology. The investigation of hydrogel swelling properties revealed their pH and temperature sensitive character. Protein loading efficiency, release profiles and the specific activity yield of the released lipase were also investigated as a function of hydrogel composition, protein content and pH, at the physiological temperature of 37 degrees C. Copolymers of N-isopropylacrylamide and itaconic acid presented high lipase loading efficiency. Another very important feature of these copolymers was that the protein release kinetic strongly depended on the pH value of the medium. The diffusion exponents values around 1 denoted that these hydrogel compositions could be adjusted to follow near zero-order kinetics. Namely, hydrogel formulations released low amounts of lipase at pH 2.20, but much higher released protein quantities were observed at pH 6.80 enabling these copolymers to be attractive candidates as site specific protein oral drug delivery systems.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid",
pages = "340-332",
number = "1-2",
volume = "436",
doi = "10.1016/j.ijpharm.2012.06.050"
}

Milašinović, N., Knežević-Jugović, Z., Milosavljević, N., Filipović, J.,& Kalagasidis Krušić, M.. (2012). Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics
Elsevier Science Bv, Amsterdam., 436(1-2), 332-340.
https://doi.org/10.1016/j.ijpharm.2012.06.050

Milašinović N, Knežević-Jugović Z, Milosavljević N, Filipović J, Kalagasidis Krušić M. Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid. in International Journal of Pharmaceutics. 2012;436(1-2):332-340.
doi:10.1016/j.ijpharm.2012.06.050 .

Milašinović, Nikola, Knežević-Jugović, Zorica, Milosavljević, Nedeljko, Filipović, Jovanka, Kalagasidis Krušić, Melina, "Controlled release of lipase from Candida rugosa loaded into hydrogels of N-isopropylacrylamide and itaconic acid" in International Journal of Pharmaceutics, 436, no. 1-2 (2012):332-340,
https://doi.org/10.1016/j.ijpharm.2012.06.050 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Milosavljević, Nedeljko
AU  - Filipović, Jovanka
AU  - Knežević-Jugović, Zorica
AU  - Kalagasidis Krušić, Melina
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2132
AB  - Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.
PB  - Springer, New York
T2  - Polymer Bulletin
T1  - Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions
EP  - 361
IS  - 3
SP  - 347
VL  - 69
DO  - 10.1007/s00289-012-0737-7
ER  - 

@article{
author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina",
year = "2012",
abstract = "Temperature and pH-sensitive hydrogels, based on N-isopropylacrylamide and itaconic acid, with varying comonomer ratios and crosslinking agent content, were prepared by free radical crosslinking copolymerization. The immobilization of lipase from Candida rugosa was carried out by post-loading entrapment method at different temperatures until equilibrium swelling was achieved. The effects of the hydrogel composition and the immobilization temperature on the hydrogel-binding capacity, immobilized lipase specific activity, as well as the enzyme leakage were studied. It was found that the NiPAAm/IA ratio, crosslinking agent concentration and the temperature at which the entrapment was performed significantly affected the hydrogel-binding capacity. The biocatalysts obtained by entrapment into hydrogel with the highest itaconic acid content at 5 A degrees C exhibited both highest binding capacity and the highest specific activity, but appeared to be less suitable for repeated uses than those obtained at 25 A degrees C.",
publisher = "Springer, New York",
journal = "Polymer Bulletin",
title = "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions",
pages = "361-347",
number = "3",
volume = "69",
doi = "10.1007/s00289-012-0737-7"
}

Milašinović, N., Milosavljević, N., Filipović, J., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2012). Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin
Springer, New York., 69(3), 347-361.
https://doi.org/10.1007/s00289-012-0737-7

Milašinović N, Milosavljević N, Filipović J, Knežević-Jugović Z, Kalagasidis Krušić M. Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions. in Polymer Bulletin. 2012;69(3):347-361.
doi:10.1007/s00289-012-0737-7 .

Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka, Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Efficient immobilization of lipase from Candida rugosa by entrapment into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels under mild conditions" in Polymer Bulletin, 69, no. 3 (2012):347-361,
https://doi.org/10.1007/s00289-012-0737-7 . .

TY  - CONF
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Isailović, Bojana
AU  - Kalagasidis Krušić, Melina
AU  - Đorđević, Verica
AU  - Knežević-Jugović, Zorica
AU  - Bugarski, Branko
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1991
AB  - The aim of this study was to investigate encapsulation processes of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads and its consecutive release. Chitosan microbeads were prepared in water-in-oil (W/O) microemulsion, while alginate-chitosan microbeads were prepared by electrostatic extrusion. The outer surface of microbeads was observed by optical microscopy. The interaction between chitosan or alginate-chitosan microbeads and extract compounds were analyzed by FT-IR. Release profiles of polyphenolic compounds from the microbeads in water was monitored. Experimental results show that the applied methods gave chitosan and alginate-chitosan microbeads with an average diameter of 390 μm and 890 μm, respectively. FT-IR analyses confirmed the presence of polyphenolic extract within the synthetized microbeads. The encapsulation efficiency was determined with respect to total polyphenolic content. Chitosan microbeads showed lower encapsulation efficiency of ∼10%, but prolonged release, compared to alginate-chitosan microbeads where encapsulation efficiency was ∼70%. The uniformity of the obtained microbeads was confirmed by optical microscopy. This study showed that, by some synthesis modification of both, chitosan and alginate-chitosan microbeads have the potential to be used for encapsulation of aqueous phenolic extracts.
PB  - 6th Central European Congress on Food, CEFood 2012
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Encapsulation of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads
EP  - 1058
SP  - 1052
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1991
ER  - 

@conference{
author = "Trifković, Kata T. and Milašinović, Nikola and Isailović, Bojana and Kalagasidis Krušić, Melina and Đorđević, Verica and Knežević-Jugović, Zorica and Bugarski, Branko",
year = "2012",
abstract = "The aim of this study was to investigate encapsulation processes of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads and its consecutive release. Chitosan microbeads were prepared in water-in-oil (W/O) microemulsion, while alginate-chitosan microbeads were prepared by electrostatic extrusion. The outer surface of microbeads was observed by optical microscopy. The interaction between chitosan or alginate-chitosan microbeads and extract compounds were analyzed by FT-IR. Release profiles of polyphenolic compounds from the microbeads in water was monitored. Experimental results show that the applied methods gave chitosan and alginate-chitosan microbeads with an average diameter of 390 μm and 890 μm, respectively. FT-IR analyses confirmed the presence of polyphenolic extract within the synthetized microbeads. The encapsulation efficiency was determined with respect to total polyphenolic content. Chitosan microbeads showed lower encapsulation efficiency of ∼10%, but prolonged release, compared to alginate-chitosan microbeads where encapsulation efficiency was ∼70%. The uniformity of the obtained microbeads was confirmed by optical microscopy. This study showed that, by some synthesis modification of both, chitosan and alginate-chitosan microbeads have the potential to be used for encapsulation of aqueous phenolic extracts.",
publisher = "6th Central European Congress on Food, CEFood 2012",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Encapsulation of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads",
pages = "1058-1052",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1991"
}

Trifković, K. T., Milašinović, N., Isailović, B., Kalagasidis Krušić, M., Đorđević, V., Knežević-Jugović, Z.,& Bugarski, B.. (2012). Encapsulation of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
6th Central European Congress on Food, CEFood 2012., 1052-1058.
https://hdl.handle.net/21.15107/rcub_technorep_1991

Trifković KT, Milašinović N, Isailović B, Kalagasidis Krušić M, Đorđević V, Knežević-Jugović Z, Bugarski B. Encapsulation of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:1052-1058.
https://hdl.handle.net/21.15107/rcub_technorep_1991 .

Trifković, Kata T., Milašinović, Nikola, Isailović, Bojana, Kalagasidis Krušić, Melina, Đorđević, Verica, Knežević-Jugović, Zorica, Bugarski, Branko, "Encapsulation of Thymus serpyllum L. aqueous extract in chitosan and alginate-chitosan microbeads" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):1052-1058,
https://hdl.handle.net/21.15107/rcub_technorep_1991 .

TY  - JOUR
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Popović, Ivanka
AU  - Filipović, Jovanka M.
AU  - Kalagasidis Krušić, Melina
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1908
AB  - Novel pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid were prepared in two steps. Chitosan was first ionically crosslinked with itaconic acid, after which a free radical polymerization and crosslinking of the chitosan/itaconic acid network was performed by adding methacrylic acid and a crosslinker in order to achieve better mechanical properties and tunable swelling. The samples were characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, dynamic mechanical analysis and the swelling ratios of the hydrogels at various pH values (2.0-8.0). The hydrogel composition is found to have a great impact on the hydrogel structure, mechanical and thermal properties, morphology and swelling kinetics. The highly porous morphology of the gels is probably connected with the bulky chitosan/itaconic acid network which reduces the degree of crosslinking in the second step of the synthesis due to steric hindrances. The gels demonstrate substantial change in buffer absorbency with change of pH, low for acid buffers and the higher for pH values above 6 where the swelling is considerably slow, thus suggesting their strong candidature for use as oral drug-delivery systems in the lower parts of the gastrointestinal tract and for drugs that require longer release times.
PB  - Wiley-Blackwell, Hoboken
T2  - Polymer International
T1  - Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid
EP  - 452
IS  - 3
SP  - 443
VL  - 60
DO  - 10.1002/pi.2967
ER  - 

@article{
author = "Milosavljević, Nedeljko and Milašinović, Nikola and Popović, Ivanka and Filipović, Jovanka M. and Kalagasidis Krušić, Melina",
year = "2011",
abstract = "Novel pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid were prepared in two steps. Chitosan was first ionically crosslinked with itaconic acid, after which a free radical polymerization and crosslinking of the chitosan/itaconic acid network was performed by adding methacrylic acid and a crosslinker in order to achieve better mechanical properties and tunable swelling. The samples were characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, dynamic mechanical analysis and the swelling ratios of the hydrogels at various pH values (2.0-8.0). The hydrogel composition is found to have a great impact on the hydrogel structure, mechanical and thermal properties, morphology and swelling kinetics. The highly porous morphology of the gels is probably connected with the bulky chitosan/itaconic acid network which reduces the degree of crosslinking in the second step of the synthesis due to steric hindrances. The gels demonstrate substantial change in buffer absorbency with change of pH, low for acid buffers and the higher for pH values above 6 where the swelling is considerably slow, thus suggesting their strong candidature for use as oral drug-delivery systems in the lower parts of the gastrointestinal tract and for drugs that require longer release times.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Polymer International",
title = "Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid",
pages = "452-443",
number = "3",
volume = "60",
doi = "10.1002/pi.2967"
}

Milosavljević, N., Milašinović, N., Popović, I., Filipović, J. M.,& Kalagasidis Krušić, M.. (2011). Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid. in Polymer International
Wiley-Blackwell, Hoboken., 60(3), 443-452.
https://doi.org/10.1002/pi.2967

Milosavljević N, Milašinović N, Popović I, Filipović JM, Kalagasidis Krušić M. Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid. in Polymer International. 2011;60(3):443-452.
doi:10.1002/pi.2967 .

Milosavljević, Nedeljko, Milašinović, Nikola, Popović, Ivanka, Filipović, Jovanka M., Kalagasidis Krušić, Melina, "Preparation and characterization of pH-sensitive hydrogels based on chitosan, itaconic acid and methacrylic acid" in Polymer International, 60, no. 3 (2011):443-452,
https://doi.org/10.1002/pi.2967 . .

TY  - JOUR
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Filipović, Jovanka M.
AU  - Kalagasidis Krušić, Melina
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1814
AB  - This paper deals with the synthesis of copolymer hydrogels of N-isopropylacrylamide, itaconic acid and chitosan. During synthesis, pH of the reaction solution and temperature were varied. It was found that the reaction at higher temperature, above lower critical solution temperature (LCST) value of N-isopropyl-acrylamide resulted in a formation of polymer rich domains or aggregates in the matrix of loosely tied network, which cause a higher degree of swelling. More-over, reaction pH also affects the degree of swelling and contraction of hydrogels owing to the different ionization of the ionic groups present in the hydrogel. The values of the diffusion exponent, n, indicate non-Fickian kinetics corresponding to diffusion and relaxation of polymer chains. These hydrogels could be classified as macroporous hydrogels. Hence, it is possible to synthesize hydrogels of desired functionality which allow their application in different fields. .
AB  - U ovom radu je prikazana sinteza kopolimernih hidrogelova N-izopropilakrilamida, itakonske kiseline i hitozana. Pri sintezi su varirani temperatura i pH vrednost rastvora za sintezu. Utvrđeno je da temperatura sinteze iznad donje kritične temperature rastvora (LCST) za NIPAM utiče na stvaranje agregata i elastičnih polimernih lanaca u mreži što omogućava veće bubrenje hidrogelova. Takođe je utvrđeno da i pH rastvora pri sintezi utiče na stepen bubrenja i kontrakcije hidrogelova, jer dolazi do različitog stepena jonizacije grupa koje ulaze u sastav hidrogela. Transport tečnosti kroz hidrogel ne prati Fick-ov zakon. Ovi hidrogelovi spadaju u grupu makroporoznih hidrogelova. Na ovaj način moguće je sintetisati hidrogelove željene funkcionalnosti što im omogućava primenu u raznim oblastima.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide
T1  - Sinteza i karakterizacija kopolimernih hidrogelova hitozana, itakonske kiseline i N-izopropilakrilamida
EP  - 666
IS  - 6
SP  - 657
VL  - 65
DO  - 10.2298/HEMIND110913072M
ER  - 

@article{
author = "Milosavljević, Nedeljko and Milašinović, Nikola and Filipović, Jovanka M. and Kalagasidis Krušić, Melina",
year = "2011",
abstract = "This paper deals with the synthesis of copolymer hydrogels of N-isopropylacrylamide, itaconic acid and chitosan. During synthesis, pH of the reaction solution and temperature were varied. It was found that the reaction at higher temperature, above lower critical solution temperature (LCST) value of N-isopropyl-acrylamide resulted in a formation of polymer rich domains or aggregates in the matrix of loosely tied network, which cause a higher degree of swelling. More-over, reaction pH also affects the degree of swelling and contraction of hydrogels owing to the different ionization of the ionic groups present in the hydrogel. The values of the diffusion exponent, n, indicate non-Fickian kinetics corresponding to diffusion and relaxation of polymer chains. These hydrogels could be classified as macroporous hydrogels. Hence, it is possible to synthesize hydrogels of desired functionality which allow their application in different fields. ., U ovom radu je prikazana sinteza kopolimernih hidrogelova N-izopropilakrilamida, itakonske kiseline i hitozana. Pri sintezi su varirani temperatura i pH vrednost rastvora za sintezu. Utvrđeno je da temperatura sinteze iznad donje kritične temperature rastvora (LCST) za NIPAM utiče na stvaranje agregata i elastičnih polimernih lanaca u mreži što omogućava veće bubrenje hidrogelova. Takođe je utvrđeno da i pH rastvora pri sintezi utiče na stepen bubrenja i kontrakcije hidrogelova, jer dolazi do različitog stepena jonizacije grupa koje ulaze u sastav hidrogela. Transport tečnosti kroz hidrogel ne prati Fick-ov zakon. Ovi hidrogelovi spadaju u grupu makroporoznih hidrogelova. Na ovaj način moguće je sintetisati hidrogelove željene funkcionalnosti što im omogućava primenu u raznim oblastima.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide, Sinteza i karakterizacija kopolimernih hidrogelova hitozana, itakonske kiseline i N-izopropilakrilamida",
pages = "666-657",
number = "6",
volume = "65",
doi = "10.2298/HEMIND110913072M"
}

Milosavljević, N., Milašinović, N., Filipović, J. M.,& Kalagasidis Krušić, M.. (2011). Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide. in Hemijska industrija
Association of Chemical Engineers of Serbia., 65(6), 657-666.
https://doi.org/10.2298/HEMIND110913072M

Milosavljević N, Milašinović N, Filipović JM, Kalagasidis Krušić M. Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide. in Hemijska industrija. 2011;65(6):657-666.
doi:10.2298/HEMIND110913072M .

Milosavljević, Nedeljko, Milašinović, Nikola, Filipović, Jovanka M., Kalagasidis Krušić, Melina, "Synthesis and characterization of copolymer hydrogels of chitosan, itaconic acid and N-isopropylacrylamide" in Hemijska industrija, 65, no. 6 (2011):657-666,
https://doi.org/10.2298/HEMIND110913072M . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Milosavljević, Nedeljko
AU  - Filipović, Jovanka M.
AU  - Knežević-Jugović, Zorica
AU  - Kalagasidis Krušić, Melina
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1806
AB  - To overcome the problems of free enzyme application as catalysts in chemical reactions, i.e., high costs of isolation and purification processes, high sensitivity to process conditions, insufficient enzyme stability etc., different immobilization techniques need to be used. Immobilization to/within solid support improves enzyme stability decreasing its denaturation. This paper deals with hydrogels of N-isopropylacrylamide and itaconic acid with incorporated nonionic surfactants (Triton X-100, Brij 30 and Tween 80) synthesized in distilled water at room temperature by free radical polymerization. These hydrogels were used as supports for immobilization of enzyme, lipase from Candida rugosa by post-entrapment method. The aim was to investigate the effect of the non-ionic surfactants on the lipase binding capacity, as well as on its hydrolytic activity. In order to characterize the obtained hydrogels, FT-IR analysis has been performed. Further, the swelling behaviour of these samples in buffer solution of pH 6.80 has been investigated. The dynamic-mechanical properties of hydrogels have been studied, too. The immobilized lipase showed somewhat reduced hydrolytic activity, compared to the activity of free lipase as well as in comparison to the lipase immobilized to the reference sample (sample synthesized under the same polymerization conditions, but in the absence of surfactants). It was concluded that the addition of surfactants increased the hydrogel mesh size. The surfactant addition did not affect the dynamic-mechanical properties of the investigated hydrogels. The largest percentage of specific activity and yield of activity were presented by the reference sample, too. It is obvious that the absence of surfactants charged groups has no influence on the lipase binding capacity, and the obtained activity yields are to be expected.
AB  - U radu je ispitana imobilizacija lipaze iz Candida rugosa u hidrogel poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu nejonskih površinski aktivnih materija. Pri sintezi je varirana vrsta i sadržaj površinski aktivnih materija. Ispitana su mehanička svojstva sintetisanih hidrogelova i urađena je FT-IR analiza. Imobilizacija lipaze je izvedena bubrenjem prethodno sintetisanih hidrogelova u rastvoru lipaze, a zatim je ispitan uticaj dodatka površinski aktivnih materija pri sintezi nosača na kapacitet uklapanja lipaze u hidrogel i na aktivnost tako uklopljene lipaze. Imobilisana lipaza iz Candida rugosa je pokazala umanjenu hidrolitičku aktivnost, kako u odnosu na aktivnost slobodne lipaze, što je i očekivano, tako i u odnosu na aktivnost lipaze imobilisane u referentni hidrogel, sintetisan bez prisustva površinski aktivnih materija.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants
T1  - Imobilizacija lipaze iz Candida rugosa u kopolimerne hidrogelove poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu površinski aktivnih materija
EP  - 673
IS  - 6
SP  - 667
VL  - 65
DO  - 10.2298/HEMIND110915063M
ER  - 

@article{
author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka M. and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina",
year = "2011",
abstract = "To overcome the problems of free enzyme application as catalysts in chemical reactions, i.e., high costs of isolation and purification processes, high sensitivity to process conditions, insufficient enzyme stability etc., different immobilization techniques need to be used. Immobilization to/within solid support improves enzyme stability decreasing its denaturation. This paper deals with hydrogels of N-isopropylacrylamide and itaconic acid with incorporated nonionic surfactants (Triton X-100, Brij 30 and Tween 80) synthesized in distilled water at room temperature by free radical polymerization. These hydrogels were used as supports for immobilization of enzyme, lipase from Candida rugosa by post-entrapment method. The aim was to investigate the effect of the non-ionic surfactants on the lipase binding capacity, as well as on its hydrolytic activity. In order to characterize the obtained hydrogels, FT-IR analysis has been performed. Further, the swelling behaviour of these samples in buffer solution of pH 6.80 has been investigated. The dynamic-mechanical properties of hydrogels have been studied, too. The immobilized lipase showed somewhat reduced hydrolytic activity, compared to the activity of free lipase as well as in comparison to the lipase immobilized to the reference sample (sample synthesized under the same polymerization conditions, but in the absence of surfactants). It was concluded that the addition of surfactants increased the hydrogel mesh size. The surfactant addition did not affect the dynamic-mechanical properties of the investigated hydrogels. The largest percentage of specific activity and yield of activity were presented by the reference sample, too. It is obvious that the absence of surfactants charged groups has no influence on the lipase binding capacity, and the obtained activity yields are to be expected., U radu je ispitana imobilizacija lipaze iz Candida rugosa u hidrogel poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu nejonskih površinski aktivnih materija. Pri sintezi je varirana vrsta i sadržaj površinski aktivnih materija. Ispitana su mehanička svojstva sintetisanih hidrogelova i urađena je FT-IR analiza. Imobilizacija lipaze je izvedena bubrenjem prethodno sintetisanih hidrogelova u rastvoru lipaze, a zatim je ispitan uticaj dodatka površinski aktivnih materija pri sintezi nosača na kapacitet uklapanja lipaze u hidrogel i na aktivnost tako uklopljene lipaze. Imobilisana lipaza iz Candida rugosa je pokazala umanjenu hidrolitičku aktivnost, kako u odnosu na aktivnost slobodne lipaze, što je i očekivano, tako i u odnosu na aktivnost lipaze imobilisane u referentni hidrogel, sintetisan bez prisustva površinski aktivnih materija.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants, Imobilizacija lipaze iz Candida rugosa u kopolimerne hidrogelove poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu površinski aktivnih materija",
pages = "673-667",
number = "6",
volume = "65",
doi = "10.2298/HEMIND110915063M"
}

Milašinović, N., Milosavljević, N., Filipović, J. M., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2011). Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants. in Hemijska industrija
Association of Chemical Engineers of Serbia., 65(6), 667-673.
https://doi.org/10.2298/HEMIND110915063M

Milašinović N, Milosavljević N, Filipović JM, Knežević-Jugović Z, Kalagasidis Krušić M. Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants. in Hemijska industrija. 2011;65(6):667-673.
doi:10.2298/HEMIND110915063M .

Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka M., Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants" in Hemijska industrija, 65, no. 6 (2011):667-673,
https://doi.org/10.2298/HEMIND110915063M . .

TY  - JOUR
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Filipović, Jovanka M.
AU  - Kalagasidis Krušić, Melina
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1642
AB  - Semi-interpenetrating networks (semi-IPNs) based on chitosan and poly(N-vinyl-2-pyrrolidone) were prepared in order to achieve better mechanical properties. The samples were characterized by FTIR, SEM, DMA analysis and the swelling behavior at different pHs (2.0-8.0). The semi- -IPNs composition was found to have a great impact on the hydrogel structure, mechanical properties, morphology and swelling kinetics. The gels demonstrated substantial change in buffer absorbency with the change of pH, high for acid buffers and lower for pH values above 6 where the swelling was considerably slow. Incorporation of PVP led to better mechanical strength of semi-IPNs while keeping a satisfactory degree of swelling, which makes them suitable for various applications.
AB  - U ovom radu je prikazana sinteza i karakterizacija semi-IPM hitozana sa poli(N-vinil-2-pirolidonom) različitog sastava i stepena umreženja. FTIR analizom je utvrđeno da interakcije između hitozana i PVP-a potiču od amino grupa hitozana i karbonilnih grupa PVP. Utvrđeno je da semi-IPM imaju poroznu strukturu i da bubrenje zavisi od pH okolnog rastvora, koncentracije umreživača i sadržaja PVP-a. Dodatak PVP-a poboljšava mehanička svojstva, ali istovremeno zadržava zadovoljavajući stepen bubrenja što sintetisane semi-IPM čini pogodnim za primenu u raznim oblastima.
PB  - Association of Chemical Engineers of Serbia
T2  - Hemijska industrija
T1  - Synthesis and characterization of semi-interpenetrating networks of chitosan and poly(N-vinyl-2-pyrrolidone)
T1  - Sinteza i karakterizacija semi-interpenetrirajućih mreža hitozana i poli(N-vinil-2-pirolidona)
EP  - 517
IS  - 6
SP  - 511
VL  - 64
DO  - 10.2298/HEMIND100827057M
ER  - 

@article{
author = "Milosavljević, Nedeljko and Milašinović, Nikola and Filipović, Jovanka M. and Kalagasidis Krušić, Melina",
year = "2010",
abstract = "Semi-interpenetrating networks (semi-IPNs) based on chitosan and poly(N-vinyl-2-pyrrolidone) were prepared in order to achieve better mechanical properties. The samples were characterized by FTIR, SEM, DMA analysis and the swelling behavior at different pHs (2.0-8.0). The semi- -IPNs composition was found to have a great impact on the hydrogel structure, mechanical properties, morphology and swelling kinetics. The gels demonstrated substantial change in buffer absorbency with the change of pH, high for acid buffers and lower for pH values above 6 where the swelling was considerably slow. Incorporation of PVP led to better mechanical strength of semi-IPNs while keeping a satisfactory degree of swelling, which makes them suitable for various applications., U ovom radu je prikazana sinteza i karakterizacija semi-IPM hitozana sa poli(N-vinil-2-pirolidonom) različitog sastava i stepena umreženja. FTIR analizom je utvrđeno da interakcije između hitozana i PVP-a potiču od amino grupa hitozana i karbonilnih grupa PVP. Utvrđeno je da semi-IPM imaju poroznu strukturu i da bubrenje zavisi od pH okolnog rastvora, koncentracije umreživača i sadržaja PVP-a. Dodatak PVP-a poboljšava mehanička svojstva, ali istovremeno zadržava zadovoljavajući stepen bubrenja što sintetisane semi-IPM čini pogodnim za primenu u raznim oblastima.",
publisher = "Association of Chemical Engineers of Serbia",
journal = "Hemijska industrija",
title = "Synthesis and characterization of semi-interpenetrating networks of chitosan and poly(N-vinyl-2-pyrrolidone), Sinteza i karakterizacija semi-interpenetrirajućih mreža hitozana i poli(N-vinil-2-pirolidona)",
pages = "517-511",
number = "6",
volume = "64",
doi = "10.2298/HEMIND100827057M"
}

Milosavljević, N., Milašinović, N., Filipović, J. M.,& Kalagasidis Krušić, M.. (2010). Synthesis and characterization of semi-interpenetrating networks of chitosan and poly(N-vinyl-2-pyrrolidone). in Hemijska industrija
Association of Chemical Engineers of Serbia., 64(6), 511-517.
https://doi.org/10.2298/HEMIND100827057M

Milosavljević N, Milašinović N, Filipović JM, Kalagasidis Krušić M. Synthesis and characterization of semi-interpenetrating networks of chitosan and poly(N-vinyl-2-pyrrolidone). in Hemijska industrija. 2010;64(6):511-517.
doi:10.2298/HEMIND100827057M .

Milosavljević, Nedeljko, Milašinović, Nikola, Filipović, Jovanka M., Kalagasidis Krušić, Melina, "Synthesis and characterization of semi-interpenetrating networks of chitosan and poly(N-vinyl-2-pyrrolidone)" in Hemijska industrija, 64, no. 6 (2010):511-517,
https://doi.org/10.2298/HEMIND100827057M . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Kalagasidis Krušić, Melina
AU  - Knežević-Jugović, Zorica
AU  - Filipović, Jovanka
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1593
AB  - Temperature- and pH-sensitive hydrogels, based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), were synthesized by free radical crosslinking copolymerization in the presence of lipase from Candida rugosa. The samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a hydrophilic model protein, lipase. These hydrogels were highly responsive to temperature and pH, at constant ionic strength. Parameters, such as the crosslinking degree and non-ionic/ionic (NiPAAm/IA) ratio, were found to impact the hydrogel structure, mechanical properties, morphology and swelling kinetics at different pH and temperatures. The hydrogels demonstrated protein loading efficiency as high as 95 wt%. Release studies of a hydrophilic model protein at a physiological temperature of 37 degrees C were performed at different pH values. High dependence of lipase release kinetics on hydrogel structure and the environmental pH was found, showing generally low release rates, lower in acidic media (pH 2.20) and higher at higher pHs (6.80). Lipase activity was retained even after treatment conditions that would provoke denaturation of the enzyme if it was not protected in the gel. The obtained hydrogels were found suitable for releasing therapeutic proteins in a controlled manner at specific sites in gastrointestinal tract.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein
EP  - 61
IS  - 1-2
SP  - 53
VL  - 383
DO  - 10.1016/j.ijpharm.2009.09.001
ER  - 

@article{
author = "Milašinović, Nikola and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica and Filipović, Jovanka",
year = "2010",
abstract = "Temperature- and pH-sensitive hydrogels, based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), were synthesized by free radical crosslinking copolymerization in the presence of lipase from Candida rugosa. The samples were characterized for their sensitivity to the changes of external conditions and the ability to control the release of a hydrophilic model protein, lipase. These hydrogels were highly responsive to temperature and pH, at constant ionic strength. Parameters, such as the crosslinking degree and non-ionic/ionic (NiPAAm/IA) ratio, were found to impact the hydrogel structure, mechanical properties, morphology and swelling kinetics at different pH and temperatures. The hydrogels demonstrated protein loading efficiency as high as 95 wt%. Release studies of a hydrophilic model protein at a physiological temperature of 37 degrees C were performed at different pH values. High dependence of lipase release kinetics on hydrogel structure and the environmental pH was found, showing generally low release rates, lower in acidic media (pH 2.20) and higher at higher pHs (6.80). Lipase activity was retained even after treatment conditions that would provoke denaturation of the enzyme if it was not protected in the gel. The obtained hydrogels were found suitable for releasing therapeutic proteins in a controlled manner at specific sites in gastrointestinal tract.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein",
pages = "61-53",
number = "1-2",
volume = "383",
doi = "10.1016/j.ijpharm.2009.09.001"
}

Milašinović, N., Kalagasidis Krušić, M., Knežević-Jugović, Z.,& Filipović, J.. (2010). Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein. in International Journal of Pharmaceutics
Elsevier Science Bv, Amsterdam., 383(1-2), 53-61.
https://doi.org/10.1016/j.ijpharm.2009.09.001

Milašinović N, Kalagasidis Krušić M, Knežević-Jugović Z, Filipović J. Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein. in International Journal of Pharmaceutics. 2010;383(1-2):53-61.
doi:10.1016/j.ijpharm.2009.09.001 .

Milašinović, Nikola, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, Filipović, Jovanka, "Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein" in International Journal of Pharmaceutics, 383, no. 1-2 (2010):53-61,
https://doi.org/10.1016/j.ijpharm.2009.09.001 . .