TY  - JOUR
AU  - Obradović, Bojana
AU  - Radisić, Milica
AU  - Vunjak-Novaković, Gordana
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1542
AB  - Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.
T2  - NATO Science for Peace and Security Series A: Chemistry and Biology
T1  - Biomimetic Approaches to Design of Tissue Engineering Bioreactors
EP  - 129
SP  - 115
DO  - 10.1007/978-90-481-8790-4-7
ER  - 

@article{
author = "Obradović, Bojana and Radisić, Milica and Vunjak-Novaković, Gordana",
year = "2010",
abstract = "Tissue engineering is an attractive strategy to address the increasing clinical need for tissue replacement. Engineered tissues can also serve as high-fidelity models for studies of development, disease and therapeutic modalities. Cultivation of three-dimensional tissue equivalents is necessarily based on the use of bioreactors, which are designed to provide controlled steady state cultivation conditions as well as required biochemical and physical regulatory signals. In this chapter, we review the design and operation of tissue engineering bioreactors, with the focus on biomimetic approaches to provide in vivo-like environments for rapid and orderly tissue development by cells cultured on a scaffold. Specifically, we focus on bioreactors for tissue engineering of two distinctly different tissues - articular cartilage and myocardium.",
journal = "NATO Science for Peace and Security Series A: Chemistry and Biology",
title = "Biomimetic Approaches to Design of Tissue Engineering Bioreactors",
pages = "129-115",
doi = "10.1007/978-90-481-8790-4-7"
}

Obradović, B., Radisić, M.,& Vunjak-Novaković, G.. (2010). Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology, 115-129.
https://doi.org/10.1007/978-90-481-8790-4-7

Obradović B, Radisić M, Vunjak-Novaković G. Biomimetic Approaches to Design of Tissue Engineering Bioreactors. in NATO Science for Peace and Security Series A: Chemistry and Biology. 2010;:115-129.
doi:10.1007/978-90-481-8790-4-7 .

Obradović, Bojana, Radisić, Milica, Vunjak-Novaković, Gordana, "Biomimetic Approaches to Design of Tissue Engineering Bioreactors" in NATO Science for Peace and Security Series A: Chemistry and Biology (2010):115-129,
https://doi.org/10.1007/978-90-481-8790-4-7 . .

TY  - JOUR
AU  - Vunjak-Novaković, Gordana
AU  - Radisić, M
AU  - Obradović, Bojana
PY  - 2006
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/920
AB  - The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick constructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs
EP  - 490
IS  - 4
SP  - 485
VL  - 81
DO  - 10.1002/jctb.1467
ER  - 

@article{
author = "Vunjak-Novaković, Gordana and Radisić, M and Obradović, Bojana",
year = "2006",
abstract = "The limited ability of cardiac muscle to regenerate after injury and the small number of organs available for transplantation motivate studies aimed at curative treatment options. Tissue engineering based on the integrated use of cells on biomaterial scaffolds in bioreactors may offer cardiac grafts suitable for surgical attachment to the myocardium or for basic research. In one of the current approaches, neonatal rat cardiomyocytes are combined with collagen sponges, gels or polyglycolic acid scaffolds (PGA). Cultivations performed in dishes, static or mixed flasks or rotating bioreactors yield constructs with a thin (100-200 mu m) peripheral layer of tissue expressing markers of cardiac differentiation and able to propagate electrical signals. The non-uniform cell distribution is a result of oxygen diffusional limitations within the constructs. Cultivations with perfusion of culture medium through the construct enhance the convective-diffusive oxygen supply and yield 1-2mm thick constructs with physiologically high and spatially uniform distribution of viable cells expressing cardiac markers. We review here a series of studies we conducted using cells seeded on three-dimensional scaffolds and cultured in several different bioreactors, to demonstrate that the bioreactor flow environment can have substantial effects on structural and functional properties of cardiac constructs.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs",
pages = "490-485",
number = "4",
volume = "81",
doi = "10.1002/jctb.1467"
}

Vunjak-Novaković, G., Radisić, M.,& Obradović, B.. (2006). Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 81(4), 485-490.
https://doi.org/10.1002/jctb.1467

Vunjak-Novaković G, Radisić M, Obradović B. Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. in Journal of Chemical Technology and Biotechnology. 2006;81(4):485-490.
doi:10.1002/jctb.1467 .

Vunjak-Novaković, Gordana, Radisić, M, Obradović, Bojana, "Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs" in Journal of Chemical Technology and Biotechnology, 81, no. 4 (2006):485-490,
https://doi.org/10.1002/jctb.1467 . .