TY  - JOUR
AU  - Petkovska, Jovana
AU  - Geskovski, Nikola
AU  - Marković, Darka
AU  - Dimova, Vesna
AU  - Mirakovski, Dejan
AU  - Radetić, Maja
AU  - Jordanov, Igor
PY  - 2024-01
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7155
AB  - Environmentally benign pH-indicating wound dressing comprised of carbohydrates chitosan (CH) and pectin (P), and anthocyanin grape (AG) dye is created via layer-by-layer assembly. Cotton fabric coated with eight bilayers of (CH-AG)4/(P-AG)4 deposited 1.97 % AG-dye. It exhibited a visible and immediate color change from pink to violet-blue while increasing its pH value from 6 to pH 7, matching the turning pH point of healing into an infected wound. Color transition of AG-dye in water-based buffers, tested by VIS-spectroscopy, shows the same color change when the pH value increased from 6 to 7. This coating imparts excellent antimicrobial activity against Gram-positive bacteria Staphylococcus aureus and yeast Candida albicans, moderate antibacterial activity against Gram-negative Escherichia coli bacteria, and no cytotoxicity on human fibroblast cells (MRC-5). This research proposes a sustainable, low-cost, and simple method for obtaining smart wound dressing that provides real-time monitoring of the wound pH.
PB  - Elsevier Ltd.
T2  - Carbohydrate Polymer Technologies and Applications
T1  - Chitosan-pectin multilayer coating with anthocyanin grape dye as pH indicating wound dressing: Synthesis and characterization
SP  - 100438
VL  - 7
DO  - 10.1016/j.carpta.2024.100438
ER  - 

@article{
author = "Petkovska, Jovana and Geskovski, Nikola and Marković, Darka and Dimova, Vesna and Mirakovski, Dejan and Radetić, Maja and Jordanov, Igor",
year = "2024-01",
abstract = "Environmentally benign pH-indicating wound dressing comprised of carbohydrates chitosan (CH) and pectin (P), and anthocyanin grape (AG) dye is created via layer-by-layer assembly. Cotton fabric coated with eight bilayers of (CH-AG)4/(P-AG)4 deposited 1.97 % AG-dye. It exhibited a visible and immediate color change from pink to violet-blue while increasing its pH value from 6 to pH 7, matching the turning pH point of healing into an infected wound. Color transition of AG-dye in water-based buffers, tested by VIS-spectroscopy, shows the same color change when the pH value increased from 6 to 7. This coating imparts excellent antimicrobial activity against Gram-positive bacteria Staphylococcus aureus and yeast Candida albicans, moderate antibacterial activity against Gram-negative Escherichia coli bacteria, and no cytotoxicity on human fibroblast cells (MRC-5). This research proposes a sustainable, low-cost, and simple method for obtaining smart wound dressing that provides real-time monitoring of the wound pH.",
publisher = "Elsevier Ltd.",
journal = "Carbohydrate Polymer Technologies and Applications",
title = "Chitosan-pectin multilayer coating with anthocyanin grape dye as pH indicating wound dressing: Synthesis and characterization",
pages = "100438",
volume = "7",
doi = "10.1016/j.carpta.2024.100438"
}

Petkovska, J., Geskovski, N., Marković, D., Dimova, V., Mirakovski, D., Radetić, M.,& Jordanov, I.. (2024-01). Chitosan-pectin multilayer coating with anthocyanin grape dye as pH indicating wound dressing: Synthesis and characterization. in Carbohydrate Polymer Technologies and Applications
Elsevier Ltd.., 7, 100438.
https://doi.org/10.1016/j.carpta.2024.100438

Petkovska J, Geskovski N, Marković D, Dimova V, Mirakovski D, Radetić M, Jordanov I. Chitosan-pectin multilayer coating with anthocyanin grape dye as pH indicating wound dressing: Synthesis and characterization. in Carbohydrate Polymer Technologies and Applications. 2024;7:100438.
doi:10.1016/j.carpta.2024.100438 .

Petkovska, Jovana, Geskovski, Nikola, Marković, Darka, Dimova, Vesna, Mirakovski, Dejan, Radetić, Maja, Jordanov, Igor, "Chitosan-pectin multilayer coating with anthocyanin grape dye as pH indicating wound dressing: Synthesis and characterization" in Carbohydrate Polymer Technologies and Applications, 7 (2024-01):100438,
https://doi.org/10.1016/j.carpta.2024.100438 . .

TY  - CONF
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Petkovska, Jovana
AU  - Mladenović, Nina
AU  - Radoičić, Marija
AU  - Vest, Natalie
AU  - Palen, Bethany
AU  - Jaime, Grunlan
AU  - Jordanov, Igor
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6866
AB  - The demand for antimicrobial textiles is rapidly
growing particularly in medical sector. In an
effort to develop a simple and efficient
antimicrobial treatment for cotton, layer-bylayer
deposition of chitosan and magnesium
lignosulfonate, followed by in situ synthesis of
silver (Ag) nanoparticles, was accomplished.
Lignin is exploited as a natural reducing and
stabilizing agent1,2, producing Ag nanoparticles
in amounts even larger than those obtained with
sodium borohydride as a strong reducing agent.
The influence of the number of bilayers (4 and
12) and initial concentration of AgNO3 solution
(10 and 20 mM) on antimicrobial activity of
cotton fabric against Gram-negative bacteria E.
coli, Gram-positive bacteria S. aureus, and C.
albicans, yeast was examined. The presence of
evenly scattered Ag nanoparticles all over the
fiber surface is confirmed by FESEM analysis
(Figure 1). The number of bilayers does not
affect the size of nanoparticles (~56 nm), but an
increase of bilayers results in greater Ag
content. The samples treated with 20 mM
AgNO3 solution contain more Ag than those
treated with 10 mM solution. The formation of
metallic Ag nanoparticles is confirmed by XPS
analysis.
Synthesized Ag nanoparticles provide excellent
antibacterial activity against bacteria S. aureus
(99.9 %), independent of the initial
concentration of AgNO3 and number of bilayers.
Larger content of Ag nanoparticles i.e., higher
initial concentration of AgNO3 solution leads to
considerably enhanced antibacterial activity
against E. coli. A similar trend is evident with
the C. albicans yeast, but the desired yeast
reduction of 99 % was not obtained.
C3  - Book of Abstracts / SICT/Plasma Tech/Tribology 2023 Joint Conferences, 26-28 Avril, 2023 - Lisbon, Portugal
T1  - Multilayer Chitosan/Lignin Nanocoating with Silver Nanoparticles for Antimicrobial Cotton
SP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6866
ER  - 

@conference{
author = "Radetić, Maja and Marković, Darka and Petkovska, Jovana and Mladenović, Nina and Radoičić, Marija and Vest, Natalie and Palen, Bethany and Jaime, Grunlan and Jordanov, Igor",
year = "2023",
abstract = "The demand for antimicrobial textiles is rapidly
growing particularly in medical sector. In an
effort to develop a simple and efficient
antimicrobial treatment for cotton, layer-bylayer
deposition of chitosan and magnesium
lignosulfonate, followed by in situ synthesis of
silver (Ag) nanoparticles, was accomplished.
Lignin is exploited as a natural reducing and
stabilizing agent1,2, producing Ag nanoparticles
in amounts even larger than those obtained with
sodium borohydride as a strong reducing agent.
The influence of the number of bilayers (4 and
12) and initial concentration of AgNO3 solution
(10 and 20 mM) on antimicrobial activity of
cotton fabric against Gram-negative bacteria E.
coli, Gram-positive bacteria S. aureus, and C.
albicans, yeast was examined. The presence of
evenly scattered Ag nanoparticles all over the
fiber surface is confirmed by FESEM analysis
(Figure 1). The number of bilayers does not
affect the size of nanoparticles (~56 nm), but an
increase of bilayers results in greater Ag
content. The samples treated with 20 mM
AgNO3 solution contain more Ag than those
treated with 10 mM solution. The formation of
metallic Ag nanoparticles is confirmed by XPS
analysis.
Synthesized Ag nanoparticles provide excellent
antibacterial activity against bacteria S. aureus
(99.9 %), independent of the initial
concentration of AgNO3 and number of bilayers.
Larger content of Ag nanoparticles i.e., higher
initial concentration of AgNO3 solution leads to
considerably enhanced antibacterial activity
against E. coli. A similar trend is evident with
the C. albicans yeast, but the desired yeast
reduction of 99 % was not obtained.",
journal = "Book of Abstracts / SICT/Plasma Tech/Tribology 2023 Joint Conferences, 26-28 Avril, 2023 - Lisbon, Portugal",
title = "Multilayer Chitosan/Lignin Nanocoating with Silver Nanoparticles for Antimicrobial Cotton",
pages = "177",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6866"
}

Radetić, M., Marković, D., Petkovska, J., Mladenović, N., Radoičić, M., Vest, N., Palen, B., Jaime, G.,& Jordanov, I.. (2023). Multilayer Chitosan/Lignin Nanocoating with Silver Nanoparticles for Antimicrobial Cotton. in Book of Abstracts / SICT/Plasma Tech/Tribology 2023 Joint Conferences, 26-28 Avril, 2023 - Lisbon, Portugal, 177.
https://hdl.handle.net/21.15107/rcub_technorep_6866

Radetić M, Marković D, Petkovska J, Mladenović N, Radoičić M, Vest N, Palen B, Jaime G, Jordanov I. Multilayer Chitosan/Lignin Nanocoating with Silver Nanoparticles for Antimicrobial Cotton. in Book of Abstracts / SICT/Plasma Tech/Tribology 2023 Joint Conferences, 26-28 Avril, 2023 - Lisbon, Portugal. 2023;:177.
https://hdl.handle.net/21.15107/rcub_technorep_6866 .

Radetić, Maja, Marković, Darka, Petkovska, Jovana, Mladenović, Nina, Radoičić, Marija, Vest, Natalie, Palen, Bethany, Jaime, Grunlan, Jordanov, Igor, "Multilayer Chitosan/Lignin Nanocoating with Silver Nanoparticles for Antimicrobial Cotton" in Book of Abstracts / SICT/Plasma Tech/Tribology 2023 Joint Conferences, 26-28 Avril, 2023 - Lisbon, Portugal (2023):177,
https://hdl.handle.net/21.15107/rcub_technorep_6866 .