Mladenović, Mihailo

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  • Mladenović, Mihailo (3)
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PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS

Bogdanović, Mihajlo; Mladenović, Dragana; Grbić, Jovana; Mladenović, Mihailo; Lazović, Saša; Đukić-Vuković, Aleksandra

(Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture, 2024)

TY  - CONF
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Grbić, Jovana
AU  - Mladenović, Mihailo
AU  - Lazović, Saša
AU  - Đukić-Vuković, Aleksandra
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7446
AB  - Kopriva (Urtica dioica) je samonikla biljka bogata flavonoidima, karotenoidima, fitosterolima,
terpenima. Ima izuzetan farmaceutski potencijal zbog svojih antiinflamatornih, antioksidativnih i
diuretskih svojstava. Koristi se u ishrani, kao caj ili u formi suvih ili tecnih ekstrakta. U cilju
pobiljšanja karakteristika, bioraspoloživosti i stabilnosti ekstrakata, koriste se ekstrakcije ekološki
prihvatljivim rastvaracima, zeleni fizicki tretmani i fermentacija probiotickim bakterijama. Zeleni
rastvaraci kao što su voda, etanol i biokompatibilni eutekticke tecnosti su pogodni za dobijanje
ekstrakata u skladu sa principima održivog razvoja. Inovativni fizicki tretmani kao netermalni
tretman plazmom mogu obezbediti bolju ekstrakciju i mikrobiološku stabilnost posebno vodenih
ekstrakata. Takode, fermentacije bakterijama, kao što je Ligilactobacillus salivarius, mogu da
metabolišu jedinjenja prisutna u ekstraktima i biotransformišu glikozide, tanine i lignane u biološki
aktivnije oblike.
U radu smo ispitivali rast i antioksidativnu aktivnost vodenih ekstrakata cveta koprive dobijenih uz
tretman netermalnom plazmom i potom fermentaciju sa L. salivarius. Ispitali smo rast L. salivarius
u medijumima sa razlicitim udelom vodenog ekstrata cveta koprive i Man Rogosa Šarp (MRS) bujona
koji je koristi kao standardni mikrobiološki medijum za kultivaciju bakterija mlecne kiseline.
Antioksidativna aktivnost dobijenih ekstrakata je ispitana DPPH metodom, pre i nakon fermentacije.
L. salivarius je pokazao najbolji rast (11 log CFU/ml) u ekstraktima obogacenim sa 25% MRS. Potom
je je ispitivan rast L. salivarius u plazma tretiranom vodenom ekstraktu koprive sa 25% MRS. Nakon
tretmana netermalnom plazmom od 3 min i naknadne fermentacije ovih ekstrakata, postignut je rast
od 9 log CFU/ml i porast antioksidativne aktivnosti od 27% DPPH metodom. Uocen je pozitivan
kombinovani efekat netermalne plazme i fermentacije na povecanje antioksidativne aktivnosti,
posebno pri kracim tretmanima netermalnom plazmom. Rezultati rada ukazuju na mogucnost
primene zelenih tretmana i fermentacije za dobijanje proizvoda ekstrakta cveta koprive obogacenih
sa L. salivarius. Plazma tretman može uticati i na mikrobiološki sastav vodenih ekstrakata, kao
važnog aspekta za primenu u prehrambenoj i farmaceutskoj industriji.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture
C3  - Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024
T1  - PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7446
ER  - 
@conference{
author = "Bogdanović, Mihajlo and Mladenović, Dragana and Grbić, Jovana and Mladenović, Mihailo and Lazović, Saša and Đukić-Vuković, Aleksandra",
year = "2024",
abstract = "Kopriva (Urtica dioica) je samonikla biljka bogata flavonoidima, karotenoidima, fitosterolima,
terpenima. Ima izuzetan farmaceutski potencijal zbog svojih antiinflamatornih, antioksidativnih i
diuretskih svojstava. Koristi se u ishrani, kao caj ili u formi suvih ili tecnih ekstrakta. U cilju
pobiljšanja karakteristika, bioraspoloživosti i stabilnosti ekstrakata, koriste se ekstrakcije ekološki
prihvatljivim rastvaracima, zeleni fizicki tretmani i fermentacija probiotickim bakterijama. Zeleni
rastvaraci kao što su voda, etanol i biokompatibilni eutekticke tecnosti su pogodni za dobijanje
ekstrakata u skladu sa principima održivog razvoja. Inovativni fizicki tretmani kao netermalni
tretman plazmom mogu obezbediti bolju ekstrakciju i mikrobiološku stabilnost posebno vodenih
ekstrakata. Takode, fermentacije bakterijama, kao što je Ligilactobacillus salivarius, mogu da
metabolišu jedinjenja prisutna u ekstraktima i biotransformišu glikozide, tanine i lignane u biološki
aktivnije oblike.
U radu smo ispitivali rast i antioksidativnu aktivnost vodenih ekstrakata cveta koprive dobijenih uz
tretman netermalnom plazmom i potom fermentaciju sa L. salivarius. Ispitali smo rast L. salivarius
u medijumima sa razlicitim udelom vodenog ekstrata cveta koprive i Man Rogosa Šarp (MRS) bujona
koji je koristi kao standardni mikrobiološki medijum za kultivaciju bakterija mlecne kiseline.
Antioksidativna aktivnost dobijenih ekstrakata je ispitana DPPH metodom, pre i nakon fermentacije.
L. salivarius je pokazao najbolji rast (11 log CFU/ml) u ekstraktima obogacenim sa 25% MRS. Potom
je je ispitivan rast L. salivarius u plazma tretiranom vodenom ekstraktu koprive sa 25% MRS. Nakon
tretmana netermalnom plazmom od 3 min i naknadne fermentacije ovih ekstrakata, postignut je rast
od 9 log CFU/ml i porast antioksidativne aktivnosti od 27% DPPH metodom. Uocen je pozitivan
kombinovani efekat netermalne plazme i fermentacije na povecanje antioksidativne aktivnosti,
posebno pri kracim tretmanima netermalnom plazmom. Rezultati rada ukazuju na mogucnost
primene zelenih tretmana i fermentacije za dobijanje proizvoda ekstrakta cveta koprive obogacenih
sa L. salivarius. Plazma tretman može uticati i na mikrobiološki sastav vodenih ekstrakata, kao
važnog aspekta za primenu u prehrambenoj i farmaceutskoj industriji.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture",
journal = "Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024",
title = "PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS",
pages = "6",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7446"
}
Bogdanović, M., Mladenović, D., Grbić, J., Mladenović, M., Lazović, S.,& Đukić-Vuković, A.. (2024). PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS. in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi = National Society of Processing and Energy in Agriculture., 6.
https://hdl.handle.net/21.15107/rcub_technorep_7446
Bogdanović M, Mladenović D, Grbić J, Mladenović M, Lazović S, Đukić-Vuković A. PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS. in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024. 2024;:6.
https://hdl.handle.net/21.15107/rcub_technorep_7446 .
Bogdanović, Mihajlo, Mladenović, Dragana, Grbić, Jovana, Mladenović, Mihailo, Lazović, Saša, Đukić-Vuković, Aleksandra, "PLAZMA AKTIVIRANI VODENI EKSTRAKATI CVETA KOPRIVE KAO SUPSTRAT ZA GAJENJE BIOMASE LIGILACTOBACILLUS SALIVARIUS" in Knjiga sažetaka = Book of Abstracts / XXXVI Naučno-stručni skup Procesna tehnika i energetika u poljoprivredi - PTEP 2024 = XXXVI Scientific - Professional Conference Processing and Energy in Agriculture - PTEP 2024, Tara, Hotel Omorika, 15 – 18. April 2024 (2024):6,
https://hdl.handle.net/21.15107/rcub_technorep_7446 .

By-products from the processing of herbs as sources of antioxidants

Mladenović, Mihailo; Grbić, Jovana; Moskovljević, Mia; Bogdanović, Mihajlo; Mladenović, Dragana; Lukić, Ivana; Đukić-Vuković, Aleksandra

(Belgrade : University, Faculty of Technology and Metallurgy, 2023)

TY  - CONF
AU  - Mladenović, Mihailo
AU  - Grbić, Jovana
AU  - Moskovljević, Mia
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Lukić, Ivana
AU  - Đukić-Vuković, Aleksandra
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6944
AB  - During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.
PB  - Belgrade : University, Faculty of Technology and Metallurgy
C3  - Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
T1  - By-products from the processing of herbs as sources of antioxidants
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6944
ER  - 
@conference{
author = "Mladenović, Mihailo and Grbić, Jovana and Moskovljević, Mia and Bogdanović, Mihajlo and Mladenović, Dragana and Lukić, Ivana and Đukić-Vuković, Aleksandra",
year = "2023",
abstract = "During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.",
publisher = "Belgrade : University, Faculty of Technology and Metallurgy",
journal = "Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade",
title = "By-products from the processing of herbs as sources of antioxidants",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6944"
}
Mladenović, M., Grbić, J., Moskovljević, M., Bogdanović, M., Mladenović, D., Lukić, I.,& Đukić-Vuković, A.. (2023). By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade
Belgrade : University, Faculty of Technology and Metallurgy., 20.
https://hdl.handle.net/21.15107/rcub_technorep_6944
Mladenović M, Grbić J, Moskovljević M, Bogdanović M, Mladenović D, Lukić I, Đukić-Vuković A. By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:20.
https://hdl.handle.net/21.15107/rcub_technorep_6944 .
Mladenović, Mihailo, Grbić, Jovana, Moskovljević, Mia, Bogdanović, Mihajlo, Mladenović, Dragana, Lukić, Ivana, Đukić-Vuković, Aleksandra, "By-products from the processing of herbs as sources of antioxidants" in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):20,
https://hdl.handle.net/21.15107/rcub_technorep_6944 .

Common nettle processing residues as a valuable source of antioxidants

Mladenović, Mihailo; Bogdanović, Mihajlo; Mladenović, Dragana; Đukić-Vuković, Aleksandra; Mojović, Ljiljana

(National Technical University of Athens, 2023)

TY  - CONF
AU  - Mladenović, Mihailo
AU  - Bogdanović, Mihajlo
AU  - Mladenović, Dragana
AU  - Đukić-Vuković, Aleksandra
AU  - Mojović, Ljiljana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6744
PB  - National Technical University of Athens
C3  - 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
T1  - Common nettle processing residues as a valuable source of antioxidants
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6744
ER  - 
@conference{
author = "Mladenović, Mihailo and Bogdanović, Mihajlo and Mladenović, Dragana and Đukić-Vuković, Aleksandra and Mojović, Ljiljana",
year = "2023",
publisher = "National Technical University of Athens",
journal = "10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece",
title = "Common nettle processing residues as a valuable source of antioxidants",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6744"
}
Mladenović, M., Bogdanović, M., Mladenović, D., Đukić-Vuković, A.,& Mojović, L.. (2023). Common nettle processing residues as a valuable source of antioxidants. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece
National Technical University of Athens..
https://hdl.handle.net/21.15107/rcub_technorep_6744
Mladenović M, Bogdanović M, Mladenović D, Đukić-Vuković A, Mojović L. Common nettle processing residues as a valuable source of antioxidants. in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece. 2023;.
https://hdl.handle.net/21.15107/rcub_technorep_6744 .
Mladenović, Mihailo, Bogdanović, Mihajlo, Mladenović, Dragana, Đukić-Vuković, Aleksandra, Mojović, Ljiljana, "Common nettle processing residues as a valuable source of antioxidants" in 10th International Conference on Sustainable Solid Waste Management, Chania2023, 21-24 June, Chania, Greece (2023),
https://hdl.handle.net/21.15107/rcub_technorep_6744 .