TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Filipović, Vladimir
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Salamon, Ivan
PY  - 2024-02
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7036
AB  - Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique
SP  - 101390
VL  - 37
DO  - 10.1016/j.scp.2023.101390
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Tadić, Vanja and Maksimović, Svetolik and Filipović, Vladimir and Dimitrijević-Branković, Suzana and Miljković, Miona and Salamon, Ivan",
year = "2024-02",
abstract = "Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique",
pages = "101390",
volume = "37",
doi = "10.1016/j.scp.2023.101390"
}

Dimitrijević, S., Milić, M., Tadić, V., Maksimović, S., Filipović, V., Dimitrijević-Branković, S., Miljković, M.,& Salamon, I.. (2024-02). Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 37, 101390.
https://doi.org/10.1016/j.scp.2023.101390

Dimitrijević S, Milić M, Tadić V, Maksimović S, Filipović V, Dimitrijević-Branković S, Miljković M, Salamon I. Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy. 2024;37:101390.
doi:10.1016/j.scp.2023.101390 .

Dimitrijević, Snežana, Milić, Marija, Tadić, Vanja, Maksimović, Svetolik, Filipović, Vladimir, Dimitrijević-Branković, Suzana, Miljković, Miona, Salamon, Ivan, "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique" in Sustainable Chemistry and Pharmacy, 37 (2024-02):101390,
https://doi.org/10.1016/j.scp.2023.101390 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Lađarević, Jelena
AU  - Onjia, Antonije
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7444
AB  - This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain
DO  - 10.1007/s11356-024-33367-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Lađarević, Jelena and Onjia, Antonije and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2024",
abstract = "This study presents an innovative approach for the reuse and recycling of waste material, brewer’s spent grain (BSG) for
creating a novel green biocatalyst. The same BSG was utilized in several consecutive steps: initially, it served as a substrate
for the cultivation and production of laccase by a novel isolated fungal strain, Coriolopsis trogii 2SMKN, then, it was reused
as a carrier for laccase immobilization, aiding in the process of azo dye decolorization and fnally, reused as recycled BSG
for the second successful laccase immobilization for six guaiacol oxidation, contributing to a zero-waste strategy. The novel
fungal strain produced laccase with a maximum activity of 171.4 U/g after 6 days of solid-state fermentation using BSG
as a substrate. The obtained laccase exhibited excellent performance in the decolorization of azo dyes, both as a free and
immobilized, at high temperatures, without addition of harmful mediators, achieving maximum decolorization efciencies
of 99.0%, 71.2%, and 61.0% for Orange G (OG), Congo Red, and Eriochrome Black T (EBT), respectively. The immobilized
laccase on BSG was successfully reused across fve cycles of azo dye decolorization process. Notably, new green biocatalyst
outperformed commercial laccase from Aspergillus spp. in the decolorization of OG and EBT. GC-MS and LC-MS revealed
azo-dye degradation products and decomposition pathway. This analysis was complemented by antimicrobial and phytotoxicity tests, which confrmed the non-toxic nature of the degradation products, indicating the potential for safe environmental
disposal.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain",
doi = "10.1007/s11356-024-33367-x"
}

Ilić, N., Davidović, S., Milić, M., Lađarević, J., Onjia, A., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2024). Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research
Springer..
https://doi.org/10.1007/s11356-024-33367-x

Ilić N, Davidović S, Milić M, Lađarević J, Onjia A, Dimitrijević-Branković S, Mihajlovski K. Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain. in Environmental Science and Pollution Research. 2024;.
doi:10.1007/s11356-024-33367-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Lađarević, Jelena, Onjia, Antonije, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "Green biocatalyst for decolorization of azo dyes from industrial wastewater: Coriolopsis trogii 2SMKN laccase immobilized on recycled brewerʼs spent grain" in Environmental Science and Pollution Research (2024),
https://doi.org/10.1007/s11356-024-33367-x . .

TY  - CHAP
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Milić, Marija
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7427
AB  - Due to the depletion of fossil fuel sources and the issues posed by climate change, there is an increasing demand for renewable and sustainable fuel alternatives. Biofuels are a viable replacement for fossil fuels since they are made from renewable biomass. Degradation and valorization of renewable biomass start in the biorefinery with the main goal of producing biofuels and biochemicals in a more sustainable way. Lignocellulosic biomass (LB) presents one of the most abundant feedstocks for low-carbon fuel production. Enzymes belonging to cellulase, xylanase, pectinase, laccase, amylase, and lipase are utilized for biomass degradation and for the biofuels production. The emphasis of this chapter will be on the smart degrading enzymes designed to produce the two low-carbon biofuels, bioethanol and biodiesel, which are mostly used in the industrial sector. The technological development of commercial enzymes involved in biofuel and chemical production is also discussed.
PB  - Cham : Springer
T2  - Biorefinery and Industry 4.0: Empowering Sustainability
T1  - The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production
EP  - 179
SP  - 113
DO  - 10.1007/978-3-031-51601-6_5
ER  - 

@inbook{
author = "Mihajlovski, Katarina and Ilić, Nevena and Milić, Marija",
year = "2024",
abstract = "Due to the depletion of fossil fuel sources and the issues posed by climate change, there is an increasing demand for renewable and sustainable fuel alternatives. Biofuels are a viable replacement for fossil fuels since they are made from renewable biomass. Degradation and valorization of renewable biomass start in the biorefinery with the main goal of producing biofuels and biochemicals in a more sustainable way. Lignocellulosic biomass (LB) presents one of the most abundant feedstocks for low-carbon fuel production. Enzymes belonging to cellulase, xylanase, pectinase, laccase, amylase, and lipase are utilized for biomass degradation and for the biofuels production. The emphasis of this chapter will be on the smart degrading enzymes designed to produce the two low-carbon biofuels, bioethanol and biodiesel, which are mostly used in the industrial sector. The technological development of commercial enzymes involved in biofuel and chemical production is also discussed.",
publisher = "Cham : Springer",
journal = "Biorefinery and Industry 4.0: Empowering Sustainability",
booktitle = "The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production",
pages = "179-113",
doi = "10.1007/978-3-031-51601-6_5"
}

Mihajlovski, K., Ilić, N.,& Milić, M.. (2024). The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production. in Biorefinery and Industry 4.0: Empowering Sustainability
Cham : Springer., 113-179.
https://doi.org/10.1007/978-3-031-51601-6_5

Mihajlovski K, Ilić N, Milić M. The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production. in Biorefinery and Industry 4.0: Empowering Sustainability. 2024;:113-179.
doi:10.1007/978-3-031-51601-6_5 .

Mihajlovski, Katarina, Ilić, Nevena, Milić, Marija, "The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production" in Biorefinery and Industry 4.0: Empowering Sustainability (2024):113-179,
https://doi.org/10.1007/978-3-031-51601-6_5 . .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Buntić, Aneta
AU  - Dimitrijević-Branković, Suzana
AU  - Filipović, Vladimir
AU  - Popović, Vera
AU  - Salamon, Ivan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7344
AB  - Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.
PB  - MDPI
T2  - Sustainability
T1  - Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost
IS  - 4
SP  - 1632
VL  - 16
DO  - 10.3390/su16041632
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Buntić, Aneta and Dimitrijević-Branković, Suzana and Filipović, Vladimir and Popović, Vera and Salamon, Ivan",
year = "2024",
abstract = "Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.",
publisher = "MDPI",
journal = "Sustainability",
title = "Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost",
number = "4",
pages = "1632",
volume = "16",
doi = "10.3390/su16041632"
}

Dimitrijević, S., Milić, M., Buntić, A., Dimitrijević-Branković, S., Filipović, V., Popović, V.,& Salamon, I.. (2024). Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. in Sustainability
MDPI., 16(4), 1632.
https://doi.org/10.3390/su16041632

Dimitrijević S, Milić M, Buntić A, Dimitrijević-Branković S, Filipović V, Popović V, Salamon I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. in Sustainability. 2024;16(4):1632.
doi:10.3390/su16041632 .

Dimitrijević, Snežana, Milić, Marija, Buntić, Aneta, Dimitrijević-Branković, Suzana, Filipović, Vladimir, Popović, Vera, Salamon, Ivan, "Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost" in Sustainability, 16, no. 4 (2024):1632,
https://doi.org/10.3390/su16041632 . .

TY  - DATA
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Buntić, Aneta
AU  - Dimitrijević-Branković, Suzana
AU  - Filipović, Vladimir
AU  - Popović, Vera
AU  - Salamon, Ivan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7345
AB  - Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.
PB  - MDPI
T2  - Sustainability
T1  - Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632
IS  - 4
SP  - 1632
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7345
ER  - 

@misc{
author = "Dimitrijević, Snežana and Milić, Marija and Buntić, Aneta and Dimitrijević-Branković, Suzana and Filipović, Vladimir and Popović, Vera and Salamon, Ivan",
year = "2024",
abstract = "Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.",
publisher = "MDPI",
journal = "Sustainability",
title = "Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632",
number = "4",
pages = "1632",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7345"
}

Dimitrijević, S., Milić, M., Buntić, A., Dimitrijević-Branković, S., Filipović, V., Popović, V.,& Salamon, I.. (2024). Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632. in Sustainability
MDPI., 16(4), 1632.
https://hdl.handle.net/21.15107/rcub_technorep_7345

Dimitrijević S, Milić M, Buntić A, Dimitrijević-Branković S, Filipović V, Popović V, Salamon I. Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632. in Sustainability. 2024;16(4):1632.
https://hdl.handle.net/21.15107/rcub_technorep_7345 .

Dimitrijević, Snežana, Milić, Marija, Buntić, Aneta, Dimitrijević-Branković, Suzana, Filipović, Vladimir, Popović, Vera, Salamon, Ivan, "Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632" in Sustainability, 16, no. 4 (2024):1632,
https://hdl.handle.net/21.15107/rcub_technorep_7345 .

TY  - JOUR
AU  - Milić, Marija
AU  - Buntić, Aneta V.
AU  - Mihajlovski, Katarina
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4981
AB  - This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.
T2  - Biomass Conversion and Biorefinery
T1  - The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization
EP  - 6759
IS  - 8
SP  - 6747
VL  - 13
DO  - 10.1007/s13399-021-01605-8
ER  - 

@article{
author = "Milić, Marija and Buntić, Aneta V. and Mihajlovski, Katarina and Ilić, Nevena and Davidović, Slađana and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "This study demonstrated the innovative biotransformation aspect of spent coffee grounds (SCG) by application of the specific enzymatic cocktail with addition of lactic acid bacteria strain Lactobacillus rhamnosus (ATCC (R) 7469 (TM)). This bioprocess induced the improvement of the most of the quality parameters in SCG, causing a considerable increase in the bioactive contents (total polyphenols, chlorogenic acid, reducing sugars, free amino acids) and antioxidant activity, in comparison to the non-fermented SCG. Under the optimal conditions, at 37 degrees C, for 24 h, with 5% v/w of the inoculum employed, the polyphenols were increased for 67% (227.3 +/- 3.3 mg GAE/g dm extract), reducing sugars 57% (277.9 +/- 7.6 mg glucose/g dm extract), and alpha-amino nitrogen 80% (161.2 +/- 9.8 mg/100 g dm extract), and the chlorogenic acid content was doubled, while the caffeine was reduced for 38%. Furthermore, the resulting product evinced a strong antioxidant activity detected via inhibition of DPPH radicals and reducing capacity assay (FRAP). The proposed technology is viable for a large-scale commercialization for utilization of easily accessible industrial residues, deriving a product with a nutritive formulation very desirable to be incorporated as an additive in food and feed industry.",
journal = "Biomass Conversion and Biorefinery",
title = "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization",
pages = "6759-6747",
number = "8",
volume = "13",
doi = "10.1007/s13399-021-01605-8"
}

Milić, M., Buntić, A. V., Mihajlovski, K., Ilić, N., Davidović, S.,& Dimitrijević-Branković, S.. (2023). The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery, 13(8), 6747-6759.
https://doi.org/10.1007/s13399-021-01605-8

Milić M, Buntić AV, Mihajlovski K, Ilić N, Davidović S, Dimitrijević-Branković S. The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization. in Biomass Conversion and Biorefinery. 2023;13(8):6747-6759.
doi:10.1007/s13399-021-01605-8 .

Milić, Marija, Buntić, Aneta V., Mihajlovski, Katarina, Ilić, Nevena, Davidović, Slađana, Dimitrijević-Branković, Suzana, "The development of a combined enzymatic and microbial fermentation as a viable technology for the spent coffee ground full utilization" in Biomass Conversion and Biorefinery, 13, no. 8 (2023):6747-6759,
https://doi.org/10.1007/s13399-021-01605-8 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Beluhan, Sunčica
AU  - Dimitrijević-Branković, Suzana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6374
AB  - Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.
PB  - MDPI
T2  - Energies
T1  - Cellulases: From Lignocellulosic Biomass to Improved Production
IS  - 8
SP  - 3598
VL  - 16
DO  - 10.3390/en16083598
ER  - 

@article{
author = "Ilić, Nevena and Milić, Marija and Beluhan, Sunčica and Dimitrijević-Branković, Suzana",
year = "2023",
abstract = "Cellulases are enzymes that are attracting worldwide attention because of their ability to degrade cellulose in the lignocellulosic biomass and transform it into highly demanded bioethanol. The enzymatic hydrolysis of cellulose by cellulases into fermentable sugars is a crucial step in biofuel production, given the complex structure of lignocellulose. Due to cellulases’ unique ability to hydrolyze the very recaltricant nature of lignocellulosic biomass, the cellulase market demand is rapidly growing. Although cellulases have been used in industrial applications for decades, constant effort is being made in the field of enzyme innovation to develop cellulase mixtures/cocktails with improved performance. Given that the main producers of cellulases are of microbial origin, there is a constant need to isolate new microorganisms as potential producers of enzymes important for biofuel production. This review provides insight into current research on improving microbial cellulase production as well as the outlook for the cellulase market with commercial cellulase preparation involved in industrial bioethanol production.",
publisher = "MDPI",
journal = "Energies",
title = "Cellulases: From Lignocellulosic Biomass to Improved Production",
number = "8",
pages = "3598",
volume = "16",
doi = "10.3390/en16083598"
}

Ilić, N., Milić, M., Beluhan, S.,& Dimitrijević-Branković, S.. (2023). Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies
MDPI., 16(8), 3598.
https://doi.org/10.3390/en16083598

Ilić N, Milić M, Beluhan S, Dimitrijević-Branković S. Cellulases: From Lignocellulosic Biomass to Improved Production. in Energies. 2023;16(8):3598.
doi:10.3390/en16083598 .

Ilić, Nevena, Milić, Marija, Beluhan, Sunčica, Dimitrijević-Branković, Suzana, "Cellulases: From Lignocellulosic Biomass to Improved Production" in Energies, 16, no. 8 (2023):3598,
https://doi.org/10.3390/en16083598 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Dimitrijević-Branković, Suzana
AU  - Mihajlovski, Katarina
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6605
AB  - Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
T1  - APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET
EP  - 186
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6605
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Dimitrijević-Branković, Suzana and Mihajlovski, Katarina",
year = "2023",
abstract = "Industrial dye effluents that contain triphenylmethane dyes are environment-threatening problem. The triphenylmethane dyes are one of the largest dye’s group that is discharged in large quantities to water bodies. Removing of them with fungal enzymes is big scientific challenge. The fungal laccases are promising tool for removing of these dyes from water bodies. In this study, the potential of crude fungal laccase from genus Ganoderma in decolorization of triphenylmethane dye, crystal violet was investigated. The crude fungal enzyme was produced using agroindustrial lignocellulosic waste, cereal mix. The effect of different substrate masses (15g, 25g and 50 g) and Cu2+ ions concentrations (0.25 mM, 0.75 mM, 1.0 mM, 2.5 mM и 5.0 mM) on lccase production were investigated. The obtained crude fungal laccase with the highest activity was used for decolorization of crystal violet at different concentrations from 20 mg/L to 50 mg/L. The crude fungal laccase from genus Ganoderma had the highest activity of 47.43 U/g, when the substrate mass of 50 g was used for enyzme production. The lower masses of substrate (15g and 25g) induced lower laccase activities of 45.07 U/g and 46.69 U/g, respectively. The initial addition of Cu2+ ions in concentration of 0.25 mM to agroindustrial waste led to decrease of laccase activity compared to the laccase activity of laccase obtained from agroindustrial waste that didn’t contain Cu2+ ions. On the other hand, the increase of Cu2+ concentration to 1.0 mM in the substrate led to increase the laccase activity. The highest laccase activity of 47.53 U/g was obtained when  the 1.0 mM Cu2+ was added to substrate, while the decrease in laccase activity was observed when 5.0 mM Cu2+ was added to the agroindustrial waste. Decolorization of crystal violet at different concentrations (20 mg/L, 30 mg/L, 40 mg/L, 50 mg/L) was carred out at pH 5 and temperature of 50 ºC for 120 min. The highest decolorization efficiency of 14.42 % was obtained in the case of the lowest dye concentration (20 mg/L), while the lowest decolorization efficiency of 3.76 % was obtained when the highest dye concentration of 50 mg/L was decolorized with crude fungal laccase for 120 min. The obtained results show that fungal crude laccases can be used for decolorization of triphenylmethane dyes, but the detailed optimization is very important for obtaining relatively high decolorization efficiencies for short time.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23",
title = "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET",
pages = "186-179",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6605"
}

Ilić, N., Milić, M., Davidović, S., Dimitrijević-Branković, S.,& Mihajlovski, K.. (2023). APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23
Zvornik : University of East Sarajevo Faculty of Technology., 179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605

Ilić N, Milić M, Davidović S, Dimitrijević-Branković S, Mihajlovski K. APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET. in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23. 2023;:179-186.
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Dimitrijević-Branković, Suzana, Mihajlovski, Katarina, "APPLICATION OF CRUDE FUNGAL LACCASE FROM GANODERMA SPP. IN DECOLORIZATION OF TRIPHENYLMETHANE DYE CRYSTAL VIOLET" in Proceedings / VIII International Congress "Engineering, Environment and Materials in Process Industry", EEM2023, Jahorina, March 20-23 (2023):179-186,
https://hdl.handle.net/21.15107/rcub_technorep_6605 .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Kostić, Anđela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5940
AB  - In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.
C3  - Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,
T1  - Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5940
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Kostić, Anđela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "In recent years, one of the biggest environmental problems is the pollution of water with colored wastewater which has negative effect on the environment and human health. Wastewaters contain complex structural compounds, such as azo dyes that used today in many industrial fields. Removing of azo dyes from wastewater using traditional methods is an extremely complex and in many cases ineffective process. In recent decades, there is a tendency towards the application of environmentally acceptable methods of removing synthetic dyes from wastewater. Method which has proven to be very effective, is the degradation of synthetic dyes using various fungal enzymes. In this study, the crude fungal laccase (31,42 UmL-1) obtained by growing fungal mycelium Ganoderma spp. on cereal mix was used for decolorization of Methyl Orange. Decolorization procedure was carried out at different temperatures (30-70 ºC) and pH (3-8) in order to determine the optimal conditions for dye decolorization. The incubation time was 180 min and every 15 min during the incubation time, the change in color intensity was monitored spectrophotometrically at 472 nm and decolorization efficiency (DE) was calculated. The optimal pH was 5 with DE of 57 % at 30 ºC, while in the case of other pH values, DE was lower. The lowest DE (1,2 %) was in the case of pH 8, which indicates that laccase activity decreases in the alkaline medium. The optimal temperature of decolorization was 50 ºC with DE of 62 % at pH 5, while the DE was lower at higher and lower temperatures, which is in accordance with the literature data on the laccase activity optimal temperature of the Ganoderma spp. The lowest DE was 35 % at 70 ºC and pH 5. The obtained results show that laccase with good decolorization properties can be obtained using cheap agro-industrial wastes, such as cereal mix. The low cost of laccase production as well as the relatively high DE in a short time may further broaden its application in wastewater treatment.",
journal = "Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,",
title = "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5940"
}

Ilić, N., Milić, M., Davidović, S., Kostić, A., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,, 80.
https://hdl.handle.net/21.15107/rcub_technorep_5940

Ilić N, Milić M, Davidović S, Kostić A, Mihajlovski K, Dimitrijević-Branković S. Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix. in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering,. 2022;:80.
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Kostić, Anđela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Decolorization of azo dye Methyl Orange with crude fungal laccase obtained by growing Ganoderma spp. on cereal mix" in Programme and Book of Abstracts, 20th Young Researchers' Conference Materials Science and Engineering, (2022):80,
https://hdl.handle.net/21.15107/rcub_technorep_5940 .

TY  - CHAP
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6145
AB  - The utilization of lignocellulosic biomass for biofuel production is a very important 
step in the transition towards to the circular economy. Within this process, the enzymatic 
hydrolysis are employed in a conversion of renewable biomass to a value-added product. 
The predominant polymer in lignocellulose is cellulose and enzymes involved in its 
degradation are cellulases. Those group of enzymes play a crucial role in biochemical degradation
of recalcitrant nature of plant lignocellulose, so marketable demand for them is rapidly growing. 
A leading enzyme manufacturers, like Novozymes, AB Enzymes, DSM, Genencor DuPont, have already developed a highly efficient cellulose-digesting enzymes cellulases for 
industrial use. Over time, those enzyme preparations have been improved and novel enzymes 
innovations in the form of enzyme cocktails have been launched on the market. Consecutively, 
this great expansion maintains a constant need among researchers for isolating and selecting 
the novel microorganisms, more potent producers of industrially powerful enzymes.
PB  - Wiley
T2  - Lignocellulose Bioconversion Through White Biotechnology
T1  - The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development
DO  - 10.1002/9781119735984.ch5
ER  - 

@inbook{
author = "Mihajlovski, Katarina and Milić, Marija",
year = "2022",
abstract = "The utilization of lignocellulosic biomass for biofuel production is a very important 
step in the transition towards to the circular economy. Within this process, the enzymatic 
hydrolysis are employed in a conversion of renewable biomass to a value-added product. 
The predominant polymer in lignocellulose is cellulose and enzymes involved in its 
degradation are cellulases. Those group of enzymes play a crucial role in biochemical degradation
of recalcitrant nature of plant lignocellulose, so marketable demand for them is rapidly growing. 
A leading enzyme manufacturers, like Novozymes, AB Enzymes, DSM, Genencor DuPont, have already developed a highly efficient cellulose-digesting enzymes cellulases for 
industrial use. Over time, those enzyme preparations have been improved and novel enzymes 
innovations in the form of enzyme cocktails have been launched on the market. Consecutively, 
this great expansion maintains a constant need among researchers for isolating and selecting 
the novel microorganisms, more potent producers of industrially powerful enzymes.",
publisher = "Wiley",
journal = "Lignocellulose Bioconversion Through White Biotechnology",
booktitle = "The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development",
doi = "10.1002/9781119735984.ch5"
}

Mihajlovski, K.,& Milić, M.. (2022). The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development. in Lignocellulose Bioconversion Through White Biotechnology
Wiley..
https://doi.org/10.1002/9781119735984.ch5

Mihajlovski K, Milić M. The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development. in Lignocellulose Bioconversion Through White Biotechnology. 2022;.
doi:10.1002/9781119735984.ch5 .

Mihajlovski, Katarina, Milić, Marija, "The Role of Plant Cell Wall Degrading Enzymes in Biorefinery Development" in Lignocellulose Bioconversion Through White Biotechnology (2022),
https://doi.org/10.1002/9781119735984.ch5 . .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Filipović, Vladimir
AU  - Milić, Marija
AU  - Dimitrijević-Branković, Suzana
AU  - Ugrenović, Vladan
AU  - Popović, Vera
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6412
AB  - U cilju proučavanja uticaja bakterija koje stimulišu rast biljaka, a koje pripadaju rodu Strep-
tomices sp , Paenibacillus sp i Hymenibacter sp , na prinos i kvalitet semena uljanog lana i crnog
kima, kao i na morfološke karakteristike biljaka, sprovedeni su dvogodišnji terenski eksperimen-
ti Tretman zemljišta mešanom kulturom bakterija različitih koncentracija je primenjen tokom
setve biljaka Pre početka setve ispitan je uticaj pojedinačnih sojeva na indeks klijavosti seme-
na uljanog lana i crnog kima Na osnovu rezultata prikazanih u radu, može se uočiti da sojevi,
CKS1 – Paenybacillus chitinolyticus, CKS3 – Hymenobacter sp , CKS7 – Streptomyces fulvissimus,
u svim ispitivanim koncentracijama, daju vrednosti indeksa klijavosti GI preko 100% kod uljanog
lana, dok je kod crnog kima takav efekat pokazao samo soj CKS7 Pozitivno dejstvo bakterijskog
preparata kod obe ispitane biljne vrste zabeleženo je merenjem prinosa semena po hektaru tre-
tiranom manjom koncentracijom bakterijskog preparata, u odnosu na odgovarajuće netretirane
uzorke (kontrolu) Pozitivna razlika zabeležena je merenjem broja čaura u uzorcima tretiranog
uljanog lana i crnog kima u odnosu na netretirani, kao i mase korena obe tretirane biljne vrste
Takođe, najveću ukupnu klijavost u odnosu na kontrolu je ostvarila varijanta uljanog lana L2 u
tretmanu manjom koncentracijom bakterija od 98,3±1,4%.
PB  - Serbian Association of Plant Breedings and Seed Producers, Belgrade
T2  - Selekcija i semenarstvo
T1  - Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima
EP  - 38
IS  - 2
SP  - 30
VL  - 28
DO  - 10.5937/SelSem2202030D
ER  - 

@article{
author = "Dimitrijević, Snežana and Filipović, Vladimir and Milić, Marija and Dimitrijević-Branković, Suzana and Ugrenović, Vladan and Popović, Vera",
year = "2022",
abstract = "U cilju proučavanja uticaja bakterija koje stimulišu rast biljaka, a koje pripadaju rodu Strep-
tomices sp , Paenibacillus sp i Hymenibacter sp , na prinos i kvalitet semena uljanog lana i crnog
kima, kao i na morfološke karakteristike biljaka, sprovedeni su dvogodišnji terenski eksperimen-
ti Tretman zemljišta mešanom kulturom bakterija različitih koncentracija je primenjen tokom
setve biljaka Pre početka setve ispitan je uticaj pojedinačnih sojeva na indeks klijavosti seme-
na uljanog lana i crnog kima Na osnovu rezultata prikazanih u radu, može se uočiti da sojevi,
CKS1 – Paenybacillus chitinolyticus, CKS3 – Hymenobacter sp , CKS7 – Streptomyces fulvissimus,
u svim ispitivanim koncentracijama, daju vrednosti indeksa klijavosti GI preko 100% kod uljanog
lana, dok je kod crnog kima takav efekat pokazao samo soj CKS7 Pozitivno dejstvo bakterijskog
preparata kod obe ispitane biljne vrste zabeleženo je merenjem prinosa semena po hektaru tre-
tiranom manjom koncentracijom bakterijskog preparata, u odnosu na odgovarajuće netretirane
uzorke (kontrolu) Pozitivna razlika zabeležena je merenjem broja čaura u uzorcima tretiranog
uljanog lana i crnog kima u odnosu na netretirani, kao i mase korena obe tretirane biljne vrste
Takođe, najveću ukupnu klijavost u odnosu na kontrolu je ostvarila varijanta uljanog lana L2 u
tretmanu manjom koncentracijom bakterija od 98,3±1,4%.",
publisher = "Serbian Association of Plant Breedings and Seed Producers, Belgrade",
journal = "Selekcija i semenarstvo",
title = "Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima",
pages = "38-30",
number = "2",
volume = "28",
doi = "10.5937/SelSem2202030D"
}

Dimitrijević, S., Filipović, V., Milić, M., Dimitrijević-Branković, S., Ugrenović, V.,& Popović, V.. (2022). Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima. in Selekcija i semenarstvo
Serbian Association of Plant Breedings and Seed Producers, Belgrade., 28(2), 30-38.
https://doi.org/10.5937/SelSem2202030D

Dimitrijević S, Filipović V, Milić M, Dimitrijević-Branković S, Ugrenović V, Popović V. Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima. in Selekcija i semenarstvo. 2022;28(2):30-38.
doi:10.5937/SelSem2202030D .

Dimitrijević, Snežana, Filipović, Vladimir, Milić, Marija, Dimitrijević-Branković, Suzana, Ugrenović, Vladan, Popović, Vera, "Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima" in Selekcija i semenarstvo, 28, no. 2 (2022):30-38,
https://doi.org/10.5937/SelSem2202030D . .

TY  - CHAP
AU  - Belozertseva, Irina
AU  - Milić, Marija
AU  - Tošić, Sonja
AU  - Saljnikov, Elmira
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6433
AB  - Detailed studies of snow and soil contamination within the influence zone of the Irkutsk Aluminium Metallurgical Plant were carried out for the period of 1996–2015. The main types of atmospheric and soil pollution and the amounts and distribution area of the pollutants were described. The study revealed that within 1 km of the aluminium smelter, the maximum fluoride concentration in the snow meltwater reached 66 mg dm−3. The relationship between technogenic soil and snowpack pollution was assessed, and their effect on some soil parameters was revealed. A standard determination of technogenic loads was carried out in relation to the significant and sensitive soil parameters. The maximum level of technogenic load was obtained by determining critical points on the “load vs. effect” curve. The values of the “dose–effect” relationship can be used to determine the maximum permissible concentration (MPC) and maximum impermissible concentration (MIC) of the potentially toxic elements in the soil. The amounts of the total forms of fluoride, aluminium and sodium were, respectively, 0.66 and 0.84 g kg−1, 82 and 93 g kg−1, and 24 and 26 g kg−1 for the upper (MPC) and lower (MIC) limits. This highlights how the soil environment is polluted with these substances emitted from the Irkutsk aluminium smelter.
PB  - Springer, Cham.
T2  - Advances in Understanding Soil Degradation
T1  - Environmental Pollution in the Vicinity of an Aluminium Smelter in Siberia
EP  - 402
SP  - 379
DO  - 10.1007/978-3-030-85682-3_18
ER  - 

@inbook{
author = "Belozertseva, Irina and Milić, Marija and Tošić, Sonja and Saljnikov, Elmira",
year = "2022",
abstract = "Detailed studies of snow and soil contamination within the influence zone of the Irkutsk Aluminium Metallurgical Plant were carried out for the period of 1996–2015. The main types of atmospheric and soil pollution and the amounts and distribution area of the pollutants were described. The study revealed that within 1 km of the aluminium smelter, the maximum fluoride concentration in the snow meltwater reached 66 mg dm−3. The relationship between technogenic soil and snowpack pollution was assessed, and their effect on some soil parameters was revealed. A standard determination of technogenic loads was carried out in relation to the significant and sensitive soil parameters. The maximum level of technogenic load was obtained by determining critical points on the “load vs. effect” curve. The values of the “dose–effect” relationship can be used to determine the maximum permissible concentration (MPC) and maximum impermissible concentration (MIC) of the potentially toxic elements in the soil. The amounts of the total forms of fluoride, aluminium and sodium were, respectively, 0.66 and 0.84 g kg−1, 82 and 93 g kg−1, and 24 and 26 g kg−1 for the upper (MPC) and lower (MIC) limits. This highlights how the soil environment is polluted with these substances emitted from the Irkutsk aluminium smelter.",
publisher = "Springer, Cham.",
journal = "Advances in Understanding Soil Degradation",
booktitle = "Environmental Pollution in the Vicinity of an Aluminium Smelter in Siberia",
pages = "402-379",
doi = "10.1007/978-3-030-85682-3_18"
}

Belozertseva, I., Milić, M., Tošić, S.,& Saljnikov, E.. (2022). Environmental Pollution in the Vicinity of an Aluminium Smelter in Siberia. in Advances in Understanding Soil Degradation
Springer, Cham.., 379-402.
https://doi.org/10.1007/978-3-030-85682-3_18

Belozertseva I, Milić M, Tošić S, Saljnikov E. Environmental Pollution in the Vicinity of an Aluminium Smelter in Siberia. in Advances in Understanding Soil Degradation. 2022;:379-402.
doi:10.1007/978-3-030-85682-3_18 .

Belozertseva, Irina, Milić, Marija, Tošić, Sonja, Saljnikov, Elmira, "Environmental Pollution in the Vicinity of an Aluminium Smelter in Siberia" in Advances in Understanding Soil Degradation (2022):379-402,
https://doi.org/10.1007/978-3-030-85682-3_18 . .

TY  - CONF
AU  - Knežević, Magdalena
AU  - Stajković-Srbinović, Olivera
AU  - Maibassova, Assel
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Delić, Dušica
AU  - Buntić, Aneta
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6434
AB  - The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.
PB  - MDPI
C3  - 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
T1  - Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6434
ER  - 

@conference{
author = "Knežević, Magdalena and Stajković-Srbinović, Olivera and Maibassova, Assel and Milić, Marija and Mihajlovski, Katarina and Delić, Dušica and Buntić, Aneta",
year = "2022",
abstract = "The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.",
publisher = "MDPI",
journal = "1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection",
title = "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6434"
}

Knežević, M., Stajković-Srbinović, O., Maibassova, A., Milić, M., Mihajlovski, K., Delić, D.,& Buntić, A.. (2022). Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
MDPI..
https://hdl.handle.net/21.15107/rcub_technorep_6434

Knežević M, Stajković-Srbinović O, Maibassova A, Milić M, Mihajlovski K, Delić D, Buntić A. Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

Knežević, Magdalena, Stajković-Srbinović, Olivera, Maibassova, Assel, Milić, Marija, Mihajlovski, Katarina, Delić, Dušica, Buntić, Aneta, "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades" in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

Filipović, V., Ugrenović, V., Maksimović, Z., Dimitrijević, S., Milić, M., Popović, V.,& Terzić, D.. (2022). Vegetativno razmnožavanje panonskog timijana (Thymus pannonicus All.) uz primenu fitohormona. .
https://hdl.handle.net/21.15107/rcub_technorep_6454

Filipović V, Ugrenović V, Maksimović Z, Dimitrijević S, Milić M, Popović V, Terzić D. Vegetativno razmnožavanje panonskog timijana (Thymus pannonicus All.) uz primenu fitohormona. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6454 .

Filipović, Vladimir, Ugrenović, Vladan, Maksimović, Zoran, Dimitrijević, Snežana, Milić, Marija, Popović, Vera, Terzić, Dragan, "Vegetativno razmnožavanje panonskog timijana (Thymus pannonicus All.) uz primenu fitohormona" (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6454 .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/20
AB  - Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Waste and Biomass Valorization
T1  - From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production
EP  - 174
IS  - 1
SP  - 165
VL  - 12
DO  - 10.1007/s12649-020-00960-3
ER  - 

@article{
author = "Mihajlovski, Katarina and Buntić, Aneta and Milić, Marija and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Waste and Biomass Valorization",
title = "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production",
pages = "174-165",
number = "1",
volume = "12",
doi = "10.1007/s12649-020-00960-3"
}

Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization
Springer, Dordrecht., 12(1), 165-174.
https://doi.org/10.1007/s12649-020-00960-3

Mihajlovski K, Buntić A, Milić M, Rajilić-Stojanović M, Dimitrijević-Branković S. From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization. 2021;12(1):165-174.
doi:10.1007/s12649-020-00960-3 .

Mihajlovski, Katarina, Buntić, Aneta, Milić, Marija, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production" in Waste and Biomass Valorization, 12, no. 1 (2021):165-174,
https://doi.org/10.1007/s12649-020-00960-3 . .

TY  - JOUR
AU  - Rudić, Suzana
AU  - Dimitrijević-Branković, Suzana
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4584
AB  - This study has developed an improved and fast microwave-assisted procedure for extraction of high yields of natural antioxidants from artichoke leaves dust. The material is derived as an abundant residue remained after processing for the industrial tea blends preparation, having no commercial value. A key factors for optimization of the total polyphenolic (TPC) and flavonoid content (TFC) and antioxidant activities have been determined by using single factor experiments along with a response surface methodology. The optimal conditions for maximizing the combination of TPC/TFC and antioxidant activity were obtained as follows: ethanol concentration 67.81%, extraction time 50 s, liquid/solid ratio 41.78 mL/g, microwave power 180 W, whereas the corresponding responses resulted in: TPC − 205.59 ± 7.30 mg GAE/g dw, TFC − 79.75 ± 1.12 mg QE/g dw, and FRAP −1008.14 ± 37.15 mmolFe2+/g dw. Only 14.7 µg/mL of the extract concentration is required to inhibit 50% of DPPH free radicals. A comparative study confirmed that the proposed procedure yielded higher TPC, TFC and antioxidant activities than available extraction methods. It highlights a several fold reduced extraction time, caring an eco-friendly principle for obtaining of antioxidants from industrial, medicinal crop residues, which would be further easily valorized in food and pharmaceutical industry.
PB  - Elsevier, Amsterdam
T2  - Separation and Purification Technology
T1  - Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants
SP  - 117714
VL  - 256
DO  - 10.1016/j.seppur.2020.117714
ER  - 

@article{
author = "Rudić, Suzana and Dimitrijević-Branković, Suzana and Dimitrijević, Snežana and Milić, Marija",
year = "2021",
abstract = "This study has developed an improved and fast microwave-assisted procedure for extraction of high yields of natural antioxidants from artichoke leaves dust. The material is derived as an abundant residue remained after processing for the industrial tea blends preparation, having no commercial value. A key factors for optimization of the total polyphenolic (TPC) and flavonoid content (TFC) and antioxidant activities have been determined by using single factor experiments along with a response surface methodology. The optimal conditions for maximizing the combination of TPC/TFC and antioxidant activity were obtained as follows: ethanol concentration 67.81%, extraction time 50 s, liquid/solid ratio 41.78 mL/g, microwave power 180 W, whereas the corresponding responses resulted in: TPC − 205.59 ± 7.30 mg GAE/g dw, TFC − 79.75 ± 1.12 mg QE/g dw, and FRAP −1008.14 ± 37.15 mmolFe2+/g dw. Only 14.7 µg/mL of the extract concentration is required to inhibit 50% of DPPH free radicals. A comparative study confirmed that the proposed procedure yielded higher TPC, TFC and antioxidant activities than available extraction methods. It highlights a several fold reduced extraction time, caring an eco-friendly principle for obtaining of antioxidants from industrial, medicinal crop residues, which would be further easily valorized in food and pharmaceutical industry.",
publisher = "Elsevier, Amsterdam",
journal = "Separation and Purification Technology",
title = "Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants",
pages = "117714",
volume = "256",
doi = "10.1016/j.seppur.2020.117714"
}

Rudić, S., Dimitrijević-Branković, S., Dimitrijević, S.,& Milić, M.. (2021). Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants. in Separation and Purification Technology
Elsevier, Amsterdam., 256, 117714.
https://doi.org/10.1016/j.seppur.2020.117714

Rudić S, Dimitrijević-Branković S, Dimitrijević S, Milić M. Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants. in Separation and Purification Technology. 2021;256:117714.
doi:10.1016/j.seppur.2020.117714 .

Rudić, Suzana, Dimitrijević-Branković, Suzana, Dimitrijević, Snežana, Milić, Marija, "Valorization of unexploited artichoke leaves dust for obtaining of extracts rich in natural antioxidants" in Separation and Purification Technology, 256 (2021):117714,
https://doi.org/10.1016/j.seppur.2020.117714 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
AU  - Pecarski, Danijela
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4853
AB  - A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.
T2  - Journal of the Serbian Chemical Society
T1  - Statistical optimization of bioethanol production from waste bread hydrolysate
EP  - 662
IS  - 7-8
SP  - 651
VL  - 86
DO  - 10.2298/JSC210308032M
ER  - 

@article{
author = "Mihajlovski, Katarina and Milić, Marija and Pecarski, Danijela and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "A recent trend in sustainable bioethanol production is the use of agricultural waste or food waste as an inexpensive and the most available feedstock. Bread waste is the major food waste that could be successfully used for the production of bioethanol. The aim of this study was to optimize ethanol production by the response surface methodology (RSM) using waste bread hydrolysate. Waste bread hydrolysate was obtained using crude hydrolytic enzymes that produce bacterial isolate Hymenobacter sp. CKS3. The influence of time of fermentation (24-72 h) and waste brewer's yeast inoculum (1-4 %) on ethanol production was studied. The optimal conditions, obtained by central composite design (CCD), were 48.6 h of fermentation and 2.85 % of inoculum. Under these conditions, a maximum of 2.06 % of ethanol concentration was reached. The obtained ethanol concentration was in good correlation, coefficient of 0.858, with yeast cell yield. The results obtained in this study imply that waste bread hydrolysate could be used as a biomass source for biofuel production with multiple benefits relating to environmental protection, reduction of production costs, and saving fossil fuels.",
journal = "Journal of the Serbian Chemical Society",
title = "Statistical optimization of bioethanol production from waste bread hydrolysate",
pages = "662-651",
number = "7-8",
volume = "86",
doi = "10.2298/JSC210308032M"
}

Mihajlovski, K., Milić, M., Pecarski, D.,& Dimitrijević-Branković, S.. (2021). Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society, 86(7-8), 651-662.
https://doi.org/10.2298/JSC210308032M

Mihajlovski K, Milić M, Pecarski D, Dimitrijević-Branković S. Statistical optimization of bioethanol production from waste bread hydrolysate. in Journal of the Serbian Chemical Society. 2021;86(7-8):651-662.
doi:10.2298/JSC210308032M .

Mihajlovski, Katarina, Milić, Marija, Pecarski, Danijela, Dimitrijević-Branković, Suzana, "Statistical optimization of bioethanol production from waste bread hydrolysate" in Journal of the Serbian Chemical Society, 86, no. 7-8 (2021):651-662,
https://doi.org/10.2298/JSC210308032M . .

TY  - JOUR
AU  - Knezevic, Magdalena M.
AU  - Stajkovic-Srbinovic, Olivera S.
AU  - Assel, Maibassova
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Delic, Dusica, I
AU  - Buntić, Aneta V.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4919
AB  - This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.
T2  - Rhizosphere
T1  - The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium
VL  - 18
DO  - 10.1016/j.rhisph.2021.100353
ER  - 

@article{
author = "Knezevic, Magdalena M. and Stajkovic-Srbinovic, Olivera S. and Assel, Maibassova and Milić, Marija and Mihajlovski, Katarina and Delic, Dusica, I and Buntić, Aneta V.",
year = "2021",
abstract = "This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.",
journal = "Rhizosphere",
title = "The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium",
volume = "18",
doi = "10.1016/j.rhisph.2021.100353"
}

Knezevic, M. M., Stajkovic-Srbinovic, O. S., Assel, M., Milić, M., Mihajlovski, K., Delic, D. I.,& Buntić, A. V.. (2021). The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium. in Rhizosphere, 18.
https://doi.org/10.1016/j.rhisph.2021.100353

Knezevic MM, Stajkovic-Srbinovic OS, Assel M, Milić M, Mihajlovski K, Delic DI, Buntić AV. The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium. in Rhizosphere. 2021;18.
doi:10.1016/j.rhisph.2021.100353 .

Knezevic, Magdalena M., Stajkovic-Srbinovic, Olivera S., Assel, Maibassova, Milić, Marija, Mihajlovski, Katarina, Delic, Dusica, I, Buntić, Aneta V., "The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium" in Rhizosphere, 18 (2021),
https://doi.org/10.1016/j.rhisph.2021.100353 . .

TY  - CONF
AU  - Ilić, Nevena
AU  - Kukučka, Andrej
AU  - Milić, Marija
AU  - Milutinović, Milica
AU  - Miljković, Miona
AU  - Davidović, Slađana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5938
AB  - Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.
C3  - Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry
T1  - Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles
EP  - 469
SP  - 461
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5938
ER  - 

@conference{
author = "Ilić, Nevena and Kukučka, Andrej and Milić, Marija and Milutinović, Milica and Miljković, Miona and Davidović, Slađana",
year = "2021",
abstract = "Antimicrobial properties of silver nanoparticles (Ag NPs) are well known and widely exploited. Various approaches have been applied for preparation of Ag NPs. Nowadays, „green“ methods are attracting more attention since they are eco-friendly and generally cheap. Moreover, incorporation of Ag NPs into various natural polymers enables preparation of biocompatible antimicrobial materials with controlled releasing of Ag NPs. Polymeric material also provides long term stability of Ag NPs. The aim of this study was to develop antimicrobial materials based on silver nanoparticles using green approach. Reduction of silver was performed by plant extracts of horsetail (Equisetum arvense L.) and cocoa (Theobroma cacao); the obtained nanoparticles were designated as EA Ag NPs and TC Ag NPs, respectively. The effect of extract concentration on particle size distribution and antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus were investigated. The smallest particles with the narrowest size distribution (between 46 nm and 91 nm) were obtained with the lowest tested concentration (10% v/v) of plant extract for both horsetail and cacao. These samples also showed the highest antibacterial activities, so they were selected for preparation of nanocomposite films based on agar-agar and chitosan. It has been shown that incorporation of both EA and TC Ag NPs significantly improved antibacterial properties of the films against E. coli (≈80% higher inhibition in comparison to the pristine agar-agar/chitosan films). In the case of S. aures, TC Ag NPs incorporated in agar-agar/chitosan films enhanced inhibition of the film for 60%. On the other side, incorporation of EA Ag NPs into agar-agar/chitosan film increased the growth inhibition for only 20%. This might be due to the contribution of cocoa extract itself, i.e. some active components that inhibit the growth of S. aureus. Our results show that agar-agar/chitosan films with incorporated green synthesized Ag NPs have potential application in the areas where usage of biodegradable and biocompatible materials with high antibacterial activity is desired, such as food package, wound healing, coatings for medical devices etc.",
journal = "Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry",
title = "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles",
pages = "469-461",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5938"
}

Ilić, N., Kukučka, A., Milić, M., Milutinović, M., Miljković, M.,& Davidović, S.. (2021). Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry, 461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938

Ilić N, Kukučka A, Milić M, Milutinović M, Miljković M, Davidović S. Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles. in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry. 2021;:461-469.
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

Ilić, Nevena, Kukučka, Andrej, Milić, Marija, Milutinović, Milica, Miljković, Miona, Davidović, Slađana, "Synthesis and characterization of agar-agar-chitosan composite films incorporated with green synthesized silver nanoparticles" in Proceedings,VII International Congress “ Engineering, Environment and Materials in Process Industry (2021):461-469,
https://hdl.handle.net/21.15107/rcub_technorep_5938 .

TY  - CONF
AU  - Ilić, Nevena
AU  - Milić, Marija
AU  - Davidović, Slađana
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5939
AB  - The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.
C3  - Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021
T1  - The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5939
ER  - 

@conference{
author = "Ilić, Nevena and Milić, Marija and Davidović, Slađana and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "The polymerization and crosslinking of various phenolic compounds induced by the laccase enzyme have been in recent years increasingly used in food industry, due to the emergence of products with improved properties, especially with increased antioxidant activity. In this regard, this study has explored the laccase-induced structural changes of two types of naturally occurring polyphenols, named gallic acid and epigallocatechin gallate, and their antioxidant activity. In the first part, the effect of the laccase of different origin - laccase of white rot fungi (Ganoderma spp.), previously grown on waste cereals and commercial laccase from Novozym® 51003, were investigated. Based on the results obtained after incubation (24 h, 50ºC), the sample containing a mixture of polyphenols, rather than individually, gave visible changes in the reading of the UV-Vis spectrum and increased antioxidant activity with small differences between commercial laccase and laccase of white rot fungi, which indicates that laccase obtained by growing fungi on waste cereals may be competitive for such a purpose. Moreover, the use of such an obtained laccase is more environmentally friendly and economically viable than use of commercial one. Further process optimization of polyphenols polymerization was performed within the method of multifactor statistical analysis (Design Expert), by using of commercial laccase, where by the effect of enzyme concentrations (0.1, 0.3 and 0.5 U/ml) and incubation time (4, 14 and 24 h) were monitored. The maximal antioxidant activity, measured by both DPPH (inhibition of 58.58% of radicals) and FRAP (176.57 mmol Fe2+/ml) was achieved by a sample containing a mixture of polyphenols and 0.3 U/ml laccase enzymes, after 14 h, at 50˚C. The results of this study revealed that careful optimization of process variables during polyphenols polymerization is extremely important for obtaining the product with desirable value added properties, which may be implemented in food and pharmaceutical industry.",
journal = "Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021",
title = "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5939"
}

Ilić, N., Milić, M., Davidović, S., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2021). The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021, 158.
https://hdl.handle.net/21.15107/rcub_technorep_5939

Ilić N, Milić M, Davidović S, Mihajlovski K, Dimitrijević-Branković S. The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme. in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021. 2021;:158.
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

Ilić, Nevena, Milić, Marija, Davidović, Slađana, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "The evaluation of the antioxidant potential during the oxidative polymerization of polyphenol compounds induced by laccase enzyme" in Book of Abstracts, The 2nd Unifood International Conference-UNIFood Conference 2021 (2021):158,
https://hdl.handle.net/21.15107/rcub_technorep_5939 .

TY  - CONF
AU  - Filipović, Vladimir
AU  - Ugrenović, Vladan
AU  - Maksimović, Zoran
AU  - Dimitrijević, Snežana
AU  - Popović, Vera
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6424
AB  - This study has explored the effect of three phytohormones application, based on α-naphthyl acetic acid, named INCIT 1, INCIT 8 and INCIT K, on the root system growing potential and rooting stimulation of Thymus pannonicus All. cuttings. This plant is highly reputable not only in a folk medicine, but also recognized in the business sector, due to a multiple uses and beneficial effects on human health.
The research was located in Veliko Središte, Vršac (Serbia) and conducted in May, 2021. The cuttings were dipped in a powdered formulation of phytohormones and placed in plastic containers pre-filled with commercial substrate. The phytohormone-free variant was taken as a control. After 2 months, the cuttings were removed and the morphological and biological properties (polyphenols content and antioxidant activity by DPPH radical and FRAP method) were measured.
Based on the morphological results, all of treated samples showed satisfactory behaviour, whereby the most suitable phytohormone to stimulate the growth of cuttings was INCIT 8, in which the average weight of the aboveground vegetative part of the plant and root mass were higher for more than 60% in relation to the control; the number of roots were about 25% higher, whereby the mass of the whole plant was almost 3 times increased. Considering the biological activities in a 5% aqueous infusions of the samples, it was found that the polyphenols content and the antioxidant activity were lowered in treated samples, with relation to the control, excluding the sample INCIT 8 where the FRAP value was slightly increased, while the inhibition of DPPH radicals was higher by around 15%.
The applied phytohormone treatment may ensure economically viable yields of standard or even improved plant quality. In the following period, it is expected that all of treated adult plants will have a higher content of biologically active substances.
PB  - University of Belgrade
C3  - UNIFood Conference
T1  - The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6424
ER  - 

@conference{
author = "Filipović, Vladimir and Ugrenović, Vladan and Maksimović, Zoran and Dimitrijević, Snežana and Popović, Vera and Mihajlovski, Katarina and Milić, Marija",
year = "2021",
abstract = "This study has explored the effect of three phytohormones application, based on α-naphthyl acetic acid, named INCIT 1, INCIT 8 and INCIT K, on the root system growing potential and rooting stimulation of Thymus pannonicus All. cuttings. This plant is highly reputable not only in a folk medicine, but also recognized in the business sector, due to a multiple uses and beneficial effects on human health.
The research was located in Veliko Središte, Vršac (Serbia) and conducted in May, 2021. The cuttings were dipped in a powdered formulation of phytohormones and placed in plastic containers pre-filled with commercial substrate. The phytohormone-free variant was taken as a control. After 2 months, the cuttings were removed and the morphological and biological properties (polyphenols content and antioxidant activity by DPPH radical and FRAP method) were measured.
Based on the morphological results, all of treated samples showed satisfactory behaviour, whereby the most suitable phytohormone to stimulate the growth of cuttings was INCIT 8, in which the average weight of the aboveground vegetative part of the plant and root mass were higher for more than 60% in relation to the control; the number of roots were about 25% higher, whereby the mass of the whole plant was almost 3 times increased. Considering the biological activities in a 5% aqueous infusions of the samples, it was found that the polyphenols content and the antioxidant activity were lowered in treated samples, with relation to the control, excluding the sample INCIT 8 where the FRAP value was slightly increased, while the inhibition of DPPH radicals was higher by around 15%.
The applied phytohormone treatment may ensure economically viable yields of standard or even improved plant quality. In the following period, it is expected that all of treated adult plants will have a higher content of biologically active substances.",
publisher = "University of Belgrade",
journal = "UNIFood Conference",
title = "The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6424"
}

Filipović, V., Ugrenović, V., Maksimović, Z., Dimitrijević, S., Popović, V., Mihajlovski, K.,& Milić, M.. (2021). The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all. in UNIFood Conference
University of Belgrade., 151.
https://hdl.handle.net/21.15107/rcub_technorep_6424

Filipović V, Ugrenović V, Maksimović Z, Dimitrijević S, Popović V, Mihajlovski K, Milić M. The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all. in UNIFood Conference. 2021;:151.
https://hdl.handle.net/21.15107/rcub_technorep_6424 .

Filipović, Vladimir, Ugrenović, Vladan, Maksimović, Zoran, Dimitrijević, Snežana, Popović, Vera, Mihajlovski, Katarina, Milić, Marija, "The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all" in UNIFood Conference (2021):151,
https://hdl.handle.net/21.15107/rcub_technorep_6424 .

TY  - CONF
AU  - Buntić, Aneta
AU  - Tošić Jojević, Sonja
AU  - Knežević, Magdalena
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Koković, Nikola
AU  - Saljnikov, Elmira
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6426
AB  - The purpose of this study was to evaluate the effect of biomass ash from combustion of soybean straw
alone or in combination with bacterial inoculums on yield of barley crop, and on soil and plant quality.
The greenhouse pot experiment included four treatments and two control soils (control without any
amendment - ØØ and control with mineral fertilizer ØØ+CAN, 0.3g CAN/pot). Treatments included
soil (3 kg/pot) and biomass ash (30 g/pot) without and with one of three selected bacterial inoculums.
Bacterial strains Streptomyces fulvissimus CKS7, Hymenobacter sp. CKS3 and Sinorhizobium (Ensifer)
meliloti 207 were used for three inoculums preparation (CKS7, CKS7+CKS3 and 207). The ash and
experimental soil samples, as well as soil and plant samples collected at the stage of crop maturity
were tested for: total N, C, S, plant available P2O5 and K2O and for the content of potentially toxic and
hazardous microelements (As, Cd, Сo, Cr, Cu, Mn, Ni, Pb, Zn). The results showed that the addition of
biomass ash significantly reduced soil acidity and increased content of plant available P and K
compared to the controls (in all treatments). Barley yield was almost twice as higher in ash treatment
and by 1.56, 1.70 and 1.81 times higher in treatments Ash+CKS7, Ash+CKS7+CKS3 and Ash+207
respectively, compared to control (ØØ). The content of potentially toxic microelements was below the
maximum allowed concentrations in the soils and shoots. The use of combinations of ash and bacterial
inoculum improved the quality of barley in terms of nitrogen content. This study showed that biomass
ash can be used as fertilizer on acidic soils with low nutrient content alone or in combination with
applied inoculants and provide an environmentally friendly approach in agriculture to reduce the
need for chemical fertilizers and solve the problem of biomass ash disposal.
PB  - Federation of Eurasian Soil Science Societies
C3  - The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey
T1  - The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality
SP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6426
ER  - 

@conference{
author = "Buntić, Aneta and Tošić Jojević, Sonja and Knežević, Magdalena and Milić, Marija and Mihajlovski, Katarina and Koković, Nikola and Saljnikov, Elmira",
year = "2021",
abstract = "The purpose of this study was to evaluate the effect of biomass ash from combustion of soybean straw
alone or in combination with bacterial inoculums on yield of barley crop, and on soil and plant quality.
The greenhouse pot experiment included four treatments and two control soils (control without any
amendment - ØØ and control with mineral fertilizer ØØ+CAN, 0.3g CAN/pot). Treatments included
soil (3 kg/pot) and biomass ash (30 g/pot) without and with one of three selected bacterial inoculums.
Bacterial strains Streptomyces fulvissimus CKS7, Hymenobacter sp. CKS3 and Sinorhizobium (Ensifer)
meliloti 207 were used for three inoculums preparation (CKS7, CKS7+CKS3 and 207). The ash and
experimental soil samples, as well as soil and plant samples collected at the stage of crop maturity
were tested for: total N, C, S, plant available P2O5 and K2O and for the content of potentially toxic and
hazardous microelements (As, Cd, Сo, Cr, Cu, Mn, Ni, Pb, Zn). The results showed that the addition of
biomass ash significantly reduced soil acidity and increased content of plant available P and K
compared to the controls (in all treatments). Barley yield was almost twice as higher in ash treatment
and by 1.56, 1.70 and 1.81 times higher in treatments Ash+CKS7, Ash+CKS7+CKS3 and Ash+207
respectively, compared to control (ØØ). The content of potentially toxic microelements was below the
maximum allowed concentrations in the soils and shoots. The use of combinations of ash and bacterial
inoculum improved the quality of barley in terms of nitrogen content. This study showed that biomass
ash can be used as fertilizer on acidic soils with low nutrient content alone or in combination with
applied inoculants and provide an environmentally friendly approach in agriculture to reduce the
need for chemical fertilizers and solve the problem of biomass ash disposal.",
publisher = "Federation of Eurasian Soil Science Societies",
journal = "The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey",
title = "The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality",
pages = "22",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6426"
}

Buntić, A., Tošić Jojević, S., Knežević, M., Milić, M., Mihajlovski, K., Koković, N.,& Saljnikov, E.. (2021). The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality. in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey
Federation of Eurasian Soil Science Societies., 22.
https://hdl.handle.net/21.15107/rcub_technorep_6426

Buntić A, Tošić Jojević S, Knežević M, Milić M, Mihajlovski K, Koković N, Saljnikov E. The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality. in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey. 2021;:22.
https://hdl.handle.net/21.15107/rcub_technorep_6426 .

Buntić, Aneta, Tošić Jojević, Sonja, Knežević, Magdalena, Milić, Marija, Mihajlovski, Katarina, Koković, Nikola, Saljnikov, Elmira, "The use of soybean biomass ash and bacterial inoculums as the treatment in acidic soils to improve soil and barley plant quality" in The International Symposium on “Soil Science and Plant Nutrition” (6th International Scientific Meetings), Samsun, Turkey (2021):22,
https://hdl.handle.net/21.15107/rcub_technorep_6426 .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Stajković-Srbinović, Olivera S.
AU  - Rasulić, Nataša V.
AU  - Delić, Dušica I.
AU  - Mihajlovski, Katarina
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4075
AB  - This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.
PB  - Springer, New York
T2  - International Journal of Environmental Science and Technology
T1  - Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production
EP  - 5890
IS  - 10
SP  - 5881
VL  - 16
DO  - 10.1007/s13762-019-02230-9
ER  - 

@article{
author = "Buntić, Aneta and Milić, Marija and Stajković-Srbinović, Olivera S. and Rasulić, Nataša V. and Delić, Dušica I. and Mihajlovski, Katarina",
year = "2019",
abstract = "This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.",
publisher = "Springer, New York",
journal = "International Journal of Environmental Science and Technology",
title = "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production",
pages = "5890-5881",
number = "10",
volume = "16",
doi = "10.1007/s13762-019-02230-9"
}

Buntić, A., Milić, M., Stajković-Srbinović, O. S., Rasulić, N. V., Delić, D. I.,& Mihajlovski, K.. (2019). Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology
Springer, New York., 16(10), 5881-5890.
https://doi.org/10.1007/s13762-019-02230-9

Buntić A, Milić M, Stajković-Srbinović OS, Rasulić NV, Delić DI, Mihajlovski K. Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology. 2019;16(10):5881-5890.
doi:10.1007/s13762-019-02230-9 .

Buntić, Aneta, Milić, Marija, Stajković-Srbinović, Olivera S., Rasulić, Nataša V., Delić, Dušica I., Mihajlovski, Katarina, "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production" in International Journal of Environmental Science and Technology, 16, no. 10 (2019):5881-5890,
https://doi.org/10.1007/s13762-019-02230-9 . .

TY  - JOUR
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Antonović, Dušan
AU  - Šiler-Marinković, Slavica
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4129
AB  - The use of the microwave-activated sugar beet pulp as an alternative adsorbent material in combination with biological processes has been applied for the colored wastewater treatment. The maximum adsorption capacity of the crystal violet dye has been achieved relatively fast, during 315 s, with no dependence of the pH, when the adsorbent mass was 15 and 0.2 g/L of the initial dye concentration. The adsorption equilibrium data fully complied with the Temkin isotherm. The afterward biological decontamination of the adsorbed dye was related to the biodegradable potential of Streptomyces microflavus CKS6 cells. The microorganism revealed strong affinity toward the dye and minimal nutritional requirements for the metabolic activity expressions. Total dye decomposition occurred after 9 h, whereby lignin peroxidase enzyme has been recognized to be mainly involved in the process. According to the phytotoxicity analysis, no harmful residues were found in the decolorized working medium.
PB  - Desalination Publ, Hopkinton
T2  - Desalination and Water Treatment
T1  - Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination
EP  - 382
SP  - 372
VL  - 169
DO  - 10.5004/dwt.2019.24677
ER  - 

@article{
author = "Buntić, Aneta and Milić, Marija and Antonović, Dušan and Šiler-Marinković, Slavica and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "The use of the microwave-activated sugar beet pulp as an alternative adsorbent material in combination with biological processes has been applied for the colored wastewater treatment. The maximum adsorption capacity of the crystal violet dye has been achieved relatively fast, during 315 s, with no dependence of the pH, when the adsorbent mass was 15 and 0.2 g/L of the initial dye concentration. The adsorption equilibrium data fully complied with the Temkin isotherm. The afterward biological decontamination of the adsorbed dye was related to the biodegradable potential of Streptomyces microflavus CKS6 cells. The microorganism revealed strong affinity toward the dye and minimal nutritional requirements for the metabolic activity expressions. Total dye decomposition occurred after 9 h, whereby lignin peroxidase enzyme has been recognized to be mainly involved in the process. According to the phytotoxicity analysis, no harmful residues were found in the decolorized working medium.",
publisher = "Desalination Publ, Hopkinton",
journal = "Desalination and Water Treatment",
title = "Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination",
pages = "382-372",
volume = "169",
doi = "10.5004/dwt.2019.24677"
}

Buntić, A., Milić, M., Antonović, D., Šiler-Marinković, S.,& Dimitrijević-Branković, S.. (2019). Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination. in Desalination and Water Treatment
Desalination Publ, Hopkinton., 169, 372-382.
https://doi.org/10.5004/dwt.2019.24677

Buntić A, Milić M, Antonović D, Šiler-Marinković S, Dimitrijević-Branković S. Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination. in Desalination and Water Treatment. 2019;169:372-382.
doi:10.5004/dwt.2019.24677 .

Buntić, Aneta, Milić, Marija, Antonović, Dušan, Šiler-Marinković, Slavica, Dimitrijević-Branković, Suzana, "Implementation of integrated adsorption and biological process in wastewater treatment for permanent dye removal and its subsequent decontamination" in Desalination and Water Treatment, 169 (2019):372-382,
https://doi.org/10.5004/dwt.2019.24677 . .