TY  - JOUR
AU  - Mandura Jarić, Ana
AU  - Haramustek, Laura
AU  - Nižić Nodilo, Laura
AU  - Vrsaljko, Domagoj
AU  - Petrović, Predrag
AU  - Kuzmić, Sunčica
AU  - Jozinović, Antun
AU  - Aladić, Krunoslav
AU  - Jokić, Stela
AU  - Šeremet, Danijela
AU  - Vojvodić Cebin, Aleksandra
AU  - Komes, Draženka
PY  - 2024-02
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7280
AB  - In this study, spray drying was used as a technological solution for the valorization of Teucrium montanum extract into carboxymethyl cellulose-based delivery systems (CMC), individually or in combination with collagen, guar gum, gum arabic, and kappa-carrageenan. The results showed that the process yield and morphological properties were positively influenced by the introduction of CMC binary blends. The employment of CMC resulted in a high encapsulation efficiency (77–96%) for all phenylethanoid glycosides (PGs) analyzed. Due to the low wettability of the microparticles, a relatively gradual in vitro release of the PGs was achieved. Infusion of the filling with hydrophilic T. montanum extract encapsulated in microparticles with high hydrophobic surface area proved to be a practical route for significant confectionery fortification (5–9 mg PGs per dw serving), ensuring prolonged interaction between the food matrix used and the extract under simulated gastrointestinal conditions. Based on sensory evaluation, the introduction of kudzu starch into the jelly matrix has shown a texture-modifying potential.
PB  - MDPI
T2  - Foods
T1  - A Novel Approach to Serving Plant-Based Confectionery—The Employment of Spray Drying in the Production of Carboxymethyl Cellulose-Based Delivery Systems Enriched with Teucrium montanum L. Extract
IS  - 3
SP  - 372
VL  - 13
DO  - 10.3390/foods13030372
ER  - 

@article{
author = "Mandura Jarić, Ana and Haramustek, Laura and Nižić Nodilo, Laura and Vrsaljko, Domagoj and Petrović, Predrag and Kuzmić, Sunčica and Jozinović, Antun and Aladić, Krunoslav and Jokić, Stela and Šeremet, Danijela and Vojvodić Cebin, Aleksandra and Komes, Draženka",
year = "2024-02",
abstract = "In this study, spray drying was used as a technological solution for the valorization of Teucrium montanum extract into carboxymethyl cellulose-based delivery systems (CMC), individually or in combination with collagen, guar gum, gum arabic, and kappa-carrageenan. The results showed that the process yield and morphological properties were positively influenced by the introduction of CMC binary blends. The employment of CMC resulted in a high encapsulation efficiency (77–96%) for all phenylethanoid glycosides (PGs) analyzed. Due to the low wettability of the microparticles, a relatively gradual in vitro release of the PGs was achieved. Infusion of the filling with hydrophilic T. montanum extract encapsulated in microparticles with high hydrophobic surface area proved to be a practical route for significant confectionery fortification (5–9 mg PGs per dw serving), ensuring prolonged interaction between the food matrix used and the extract under simulated gastrointestinal conditions. Based on sensory evaluation, the introduction of kudzu starch into the jelly matrix has shown a texture-modifying potential.",
publisher = "MDPI",
journal = "Foods",
title = "A Novel Approach to Serving Plant-Based Confectionery—The Employment of Spray Drying in the Production of Carboxymethyl Cellulose-Based Delivery Systems Enriched with Teucrium montanum L. Extract",
number = "3",
pages = "372",
volume = "13",
doi = "10.3390/foods13030372"
}

Mandura Jarić, A., Haramustek, L., Nižić Nodilo, L., Vrsaljko, D., Petrović, P., Kuzmić, S., Jozinović, A., Aladić, K., Jokić, S., Šeremet, D., Vojvodić Cebin, A.,& Komes, D.. (2024-02). A Novel Approach to Serving Plant-Based Confectionery—The Employment of Spray Drying in the Production of Carboxymethyl Cellulose-Based Delivery Systems Enriched with Teucrium montanum L. Extract. in Foods
MDPI., 13(3), 372.
https://doi.org/10.3390/foods13030372

Mandura Jarić A, Haramustek L, Nižić Nodilo L, Vrsaljko D, Petrović P, Kuzmić S, Jozinović A, Aladić K, Jokić S, Šeremet D, Vojvodić Cebin A, Komes D. A Novel Approach to Serving Plant-Based Confectionery—The Employment of Spray Drying in the Production of Carboxymethyl Cellulose-Based Delivery Systems Enriched with Teucrium montanum L. Extract. in Foods. 2024;13(3):372.
doi:10.3390/foods13030372 .

Mandura Jarić, Ana, Haramustek, Laura, Nižić Nodilo, Laura, Vrsaljko, Domagoj, Petrović, Predrag, Kuzmić, Sunčica, Jozinović, Antun, Aladić, Krunoslav, Jokić, Stela, Šeremet, Danijela, Vojvodić Cebin, Aleksandra, Komes, Draženka, "A Novel Approach to Serving Plant-Based Confectionery—The Employment of Spray Drying in the Production of Carboxymethyl Cellulose-Based Delivery Systems Enriched with Teucrium montanum L. Extract" in Foods, 13, no. 3 (2024-02):372,
https://doi.org/10.3390/foods13030372 . .

TY  - CONF
AU  - Ugrinović, Vukašin
AU  - Marković, Maja
AU  - Petrović, Predrag
AU  - Veljović, Đorđe
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7393
AB  - INTRODUCTION: Hydrogels,  characterized  by  their  three-dimensional  hydrophilic  polymer  networks  capable  of retaining substantial amounts of water or biological fluids, hold significant promise for biomedical and pharmaceutical applications.  While  numerous  polymers  have  been  explored  for  hydrogel  development,  those  derived  from  natural sources  possess  inherent  advantages  due  to  their  abundance,  affordability,  biocompatibility,  and  biodegradability. Gelatin, a widely used natural polymer in biomedicine, standsout for its cost-effectiveness, compatibility with biological systems,  and  degradability.  Hydroxypropyl  methylcellulose  (HPMC),  a  cellulose  derivative,  exhibits  hydrophilicity, biodegradability,  and  biocompatibility.  However,  natural  polymer-based  hydrogels  often  exhibit  low  mechanical strength  and  solubility  in  physiological  conditions,  necessitating  innovative  cross-linking  strategies  to  enhance  their functionality. Citric acid (CA) emerges as a promising crosslinking agent owing to its affordability andnon-toxic nature.EXPERIMENTAL:HPMC-G hydrogels were synthesized by dissolving gelatin (0.05 g) and HPMC (0.1 g) with specified amounts of CA and sodium hypophosphitein 1 ml of distilled water in a reaction vessel. The mixture was homogenized, poured into a Teflon mold, and frozen at -20°C for 24 hours. After lyophilization, the hydrogels underwent crosslinking at 160°C for 7 minutes. By varying the CA content from 0%to 40% (w/w) while maintaining constant temperature and time, the optimal CA/HPMC ratio was determined. Subsequently, various heat treatments (140–180°C, 3-12 minutes) were applied to explore the optimal curing conditions.RESULTS AND DISCUSSION:The investigation elucidates how the CA/HPMC ratio and curing conditions impact the physicochemical and mechanical propertiesof HPMC-G hydrogels. Swelling tests and compressive mechanical property evaluations reveal that the incorporation of citric acid, along with increases in curing temperature and time, enhances compressive  modulus  and  degradation  stability  while  reducing  equilibrium  swelling.  Scanning  electron  microscopyanalysis reveals a highly porous microstructure in the resulting hydrogels, while differential scanning calorimetrycurves indicate the formation of strong interactions between gelatin and HPMC.CONCLUSIONS:The utilization of these materials notonly contributes to environmental conservation efforts but also drives the advancement of eco-friendly technology, aligning with the principles of the circular economy. Moreover, it offers promising avenues for innovative solutions in potential biomedicalapplications.
PB  - Savez hemijskih inženjera Srbije
C3  - Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
T1  - Citric acid-crosslinked gelatin/hydroxypropyl methylcellulose hydrogels for biomedical applications
IS  - 1S
SP  - 29
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7393
ER  - 

@conference{
author = "Ugrinović, Vukašin and Marković, Maja and Petrović, Predrag and Veljović, Đorđe",
year = "2024",
abstract = "INTRODUCTION: Hydrogels,  characterized  by  their  three-dimensional  hydrophilic  polymer  networks  capable  of retaining substantial amounts of water or biological fluids, hold significant promise for biomedical and pharmaceutical applications.  While  numerous  polymers  have  been  explored  for  hydrogel  development,  those  derived  from  natural sources  possess  inherent  advantages  due  to  their  abundance,  affordability,  biocompatibility,  and  biodegradability. Gelatin, a widely used natural polymer in biomedicine, standsout for its cost-effectiveness, compatibility with biological systems,  and  degradability.  Hydroxypropyl  methylcellulose  (HPMC),  a  cellulose  derivative,  exhibits  hydrophilicity, biodegradability,  and  biocompatibility.  However,  natural  polymer-based  hydrogels  often  exhibit  low  mechanical strength  and  solubility  in  physiological  conditions,  necessitating  innovative  cross-linking  strategies  to  enhance  their functionality. Citric acid (CA) emerges as a promising crosslinking agent owing to its affordability andnon-toxic nature.EXPERIMENTAL:HPMC-G hydrogels were synthesized by dissolving gelatin (0.05 g) and HPMC (0.1 g) with specified amounts of CA and sodium hypophosphitein 1 ml of distilled water in a reaction vessel. The mixture was homogenized, poured into a Teflon mold, and frozen at -20°C for 24 hours. After lyophilization, the hydrogels underwent crosslinking at 160°C for 7 minutes. By varying the CA content from 0%to 40% (w/w) while maintaining constant temperature and time, the optimal CA/HPMC ratio was determined. Subsequently, various heat treatments (140–180°C, 3-12 minutes) were applied to explore the optimal curing conditions.RESULTS AND DISCUSSION:The investigation elucidates how the CA/HPMC ratio and curing conditions impact the physicochemical and mechanical propertiesof HPMC-G hydrogels. Swelling tests and compressive mechanical property evaluations reveal that the incorporation of citric acid, along with increases in curing temperature and time, enhances compressive  modulus  and  degradation  stability  while  reducing  equilibrium  swelling.  Scanning  electron  microscopyanalysis reveals a highly porous microstructure in the resulting hydrogels, while differential scanning calorimetrycurves indicate the formation of strong interactions between gelatin and HPMC.CONCLUSIONS:The utilization of these materials notonly contributes to environmental conservation efforts but also drives the advancement of eco-friendly technology, aligning with the principles of the circular economy. Moreover, it offers promising avenues for innovative solutions in potential biomedicalapplications.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts",
title = "Citric acid-crosslinked gelatin/hydroxypropyl methylcellulose hydrogels for biomedical applications",
number = "1S",
pages = "29",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7393"
}

Ugrinović, V., Marković, M., Petrović, P.,& Veljović, Đ.. (2024). Citric acid-crosslinked gelatin/hydroxypropyl methylcellulose hydrogels for biomedical applications. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts
Savez hemijskih inženjera Srbije., 78(1S), 29.
https://hdl.handle.net/21.15107/rcub_technorep_7393

Ugrinović V, Marković M, Petrović P, Veljović Đ. Citric acid-crosslinked gelatin/hydroxypropyl methylcellulose hydrogels for biomedical applications. in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts. 2024;78(1S):29.
https://hdl.handle.net/21.15107/rcub_technorep_7393 .

Ugrinović, Vukašin, Marković, Maja, Petrović, Predrag, Veljović, Đorđe, "Citric acid-crosslinked gelatin/hydroxypropyl methylcellulose hydrogels for biomedical applications" in Hemijska industrija - Supplementary Issue - ExcellMater Conference 2024 Abstracts, 78, no. 1S (2024):29,
https://hdl.handle.net/21.15107/rcub_technorep_7393 .

Knežević Jugović, Z., Đorđević, V., Antov, M., Šekuljica, N., Mijalković, J., Jakovetić Tanasković, S., Balanč, B., Petrović, P., Salević-Jelić, A., Rakić, V., Nedović, V.,& Bugarski, B.. (2023). Колачићи на бази протеина нове генерације – протеина из лишћа уљарица. in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа
Београд : Центар за промоцију науке., 37.
https://hdl.handle.net/21.15107/rcub_technorep_7078

Knežević Jugović Z, Đorđević V, Antov M, Šekuljica N, Mijalković J, Jakovetić Tanasković S, Balanč B, Petrović P, Salević-Jelić A, Rakić V, Nedović V, Bugarski B. Колачићи на бази протеина нове генерације – протеина из лишћа уљарица. in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа. 2023;:37.
https://hdl.handle.net/21.15107/rcub_technorep_7078 .

Knežević Jugović, Zorica, Đorđević, Verica, Antov, Mirjana, Šekuljica, Nataša, Mijalković, Jelena, Jakovetić Tanasković, Sonja, Balanč, Bojana, Petrović, Predrag, Salević-Jelić, Ana, Rakić, Vesna, Nedović, Viktor, Bugarski, Branko, "Колачићи на бази протеина нове генерације – протеина из лишћа уљарица" in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа (2023):37,
https://hdl.handle.net/21.15107/rcub_technorep_7078 .

TY  - JOUR
AU  - Mijin, Nemanja
AU  - Milošević, Jelica
AU  - Stevanović, Sanja
AU  - Petrović, Predrag
AU  - Lolić, Aleksandar
AU  - Urbic, Tomaz
AU  - Polović, Natalija
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5268
AB  - The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.
PB  - Elsevier B.V.
T2  - Food Hydrocolloids
T1  - Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin
SP  - 108292
VL  - 136
DO  - 10.1016/j.foodhyd.2022.108292
ER  - 

@article{
author = "Mijin, Nemanja and Milošević, Jelica and Stevanović, Sanja and Petrović, Predrag and Lolić, Aleksandar and Urbic, Tomaz and Polović, Natalija",
year = "2023",
abstract = "The aggregation of proteins into fibrillar, amyloid-like aggregates generally results in an improved, positive effect on various techno-functional properties within food products, such as gelation, emulsification, and foam stabilization. These highly stable structures, characterized by their repetitive, β-sheet rich motifs, may develop as the result of the thermal treatment of protein-rich food products. Heavy metal ions can influence amyloid-like aggregation of food proteins. Lead(II) and cadmium(II) represent some of the most abundant and common environmental water and food pollutants. In this work, the influence of heavy metal ions, lead and cadmium on amyloid-like aggregation of ovalbumin at high temperatures (90 °C) and under acidic conditions (pH 2.0) was investigated. Ovalbumin is used as a general model for how heavy metals can affect amyloid-like aggregation of a food protein. Structural changes were monitored via Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence, Fourier-Transform infrared spectroscopy, atomic force microscopy, dynamic light scattering, as well as computational analyses. The obtained results indicate that the added heavy metal ions bind to different sites within ovalbumin prior to thermal treatment. Lead binding sites are closer to the hydrophobic regions of an protein, while cadmium ion binding sites are more exposed. This specific binding of metal ions affects the morphologies of amyloid-like aggregates, resulting in lead-induced branching of amyloid-like fibrils, or cadmium-induced tangling of fibrils into dense amyloid clusters. This additive effect of heavy metal ions is most evident in ovalbumin samples which contain a mixture of both heavy metal ions.",
publisher = "Elsevier B.V.",
journal = "Food Hydrocolloids",
title = "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin",
pages = "108292",
volume = "136",
doi = "10.1016/j.foodhyd.2022.108292"
}

Mijin, N., Milošević, J., Stevanović, S., Petrović, P., Lolić, A., Urbic, T.,& Polović, N.. (2023). Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids
Elsevier B.V.., 136, 108292.
https://doi.org/10.1016/j.foodhyd.2022.108292

Mijin N, Milošević J, Stevanović S, Petrović P, Lolić A, Urbic T, Polović N. Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin. in Food Hydrocolloids. 2023;136:108292.
doi:10.1016/j.foodhyd.2022.108292 .

Mijin, Nemanja, Milošević, Jelica, Stevanović, Sanja, Petrović, Predrag, Lolić, Aleksandar, Urbic, Tomaz, Polović, Natalija, "Amyloid-like aggregation influenced by lead(II) and cadmium(II) ions in hen egg white ovalbumin" in Food Hydrocolloids, 136 (2023):108292,
https://doi.org/10.1016/j.foodhyd.2022.108292 . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Volić, Mina
AU  - Obradović, Nataša
AU  - Balanč, Bojana
AU  - Pravilović, Radoslava
AU  - Bugarski, Branko
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6076
AB  - In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.
PB  - University in Banjaluka, Faculty of Technology
C3  - XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA
T1  - The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts
EP  - 104
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6076
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Volić, Mina and Obradović, Nataša and Balanč, Bojana and Pravilović, Radoslava and Bugarski, Branko",
year = "2023",
abstract = "In the present study, Serpylli herba's waste extracts were prepared using 50% ethanol (pH 6) and 50%
ethanol with hydrochloric acid (pH 1.2), using maceration (25 °C) and heat-assisted extraction (HAE,
80 °C). The best extraction conditions were determined via analyzing total polyphenol (TPC) and
flavonoid contents (TFC), antioxidant capacity, extraction yield, zeta potential, density, and surface
tension. In maceration and HAE, TPC were 1.18±0.06 and 1.43±0.05 mg GAE/mL (pH 6), and
0.69±0.05 and 0.86±0.02 mg GAE/mL (pH 1.2). The same trend is noticed in the case of TFC:
0.33±0.01 and 0.42±0.02 mg CE/mL (pH 6) and 0.24±0.01 and 0.27±0.01 mg CE/mL (pH 1.2). ABTS
radical scavenging activity was 775.0±3.5 and 623.8±5.1 mmol Trolox/mL (pH 6), and 269.2±3.1
and 327.9±7.7 mmol Trolox/mL (pH 1.2). In DPPH assay, IC50 was 1.64±0.04 and 1.48±0.03 mg/mL
(pH 6), and 7.59±0.07 and 5.11±0.09 mg/mL (pH 1.2). Extraction yield was 0.541±0.049 and
0.583±0.019% (pH 6), and 0.938±0.057 and 0.889±0.060% (pH 1.2). Zeta potential was -2.58±0.02
and -2.08±0.10 mV (pH 6), and 1.49±0.37 and 1.08±0.09 mV (pH 1.2). Density was 0.945±0.001
and 0.949±0.001 g/mL (pH 6), and 1.008±0.001 and 1.010±0.001 g/mL (pH 1.2). Surface tension
was 29.2±0.3 and 30.5±1.0 mN/m (pH 6), and 32.6±2.3 and 36.5±2.3 mN/m (pH 1.2). The best
properties were determined in the extract obtained in HAE at pH 6. The aim of this study is to provide
evidence of the physico-chemical properties of Serpylli herba's waste extracts that can add value and
improve the quality of existing food, pharmaceutical, and cosmetic products.",
publisher = "University in Banjaluka, Faculty of Technology",
journal = "XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA",
title = "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts",
pages = "104-96",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6076"
}

Jovanović, A. A., Petrović, P., Volić, M., Obradović, N., Balanč, B., Pravilović, R.,& Bugarski, B.. (2023). The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA
University in Banjaluka, Faculty of Technology., 96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076

Jovanović AA, Petrović P, Volić M, Obradović N, Balanč B, Pravilović R, Bugarski B. The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts. in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA. 2023;:96-104.
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

Jovanović, Aleksandra A., Petrović, Predrag, Volić, Mina, Obradović, Nataša, Balanč, Bojana, Pravilović, Radoslava, Bugarski, Branko, "The impact of pH value and temperature on polyphenol recovery, antioxidant capacity and physical properties of Serpylli herba's waste extracts" in XIV CONFERENCE OF CHEMISTS, TECHNOLOGISTS AND
ENVIRONMENTALISTS OF REPUBLIC OF SRPSKA
BOOK OF PROCEEDINGS, OCTOBER 21-22, 2022, BANJA LUKA (2023):96-104,
https://hdl.handle.net/21.15107/rcub_technorep_6076 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Petković, Miloš
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6734
AB  - The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).
PB  - John Wiley and Sons Inc.
T2  - Chemistry and Biodiversity
T1  - Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites
SP  - e202301044
DO  - 10.1002/cbdv.202301044
ER  - 

@article{
author = "Milutinović, Violeta and Petrović, Predrag and Petković, Miloš and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).",
publisher = "John Wiley and Sons Inc.",
journal = "Chemistry and Biodiversity",
title = "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites",
pages = "e202301044",
doi = "10.1002/cbdv.202301044"
}

Milutinović, V., Petrović, P., Petković, M., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity
John Wiley and Sons Inc.., e202301044.
https://doi.org/10.1002/cbdv.202301044

Milutinović V, Petrović P, Petković M, Klaus A, Ušjak L, Niketić M, Petrović S. Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity. 2023;:e202301044.
doi:10.1002/cbdv.202301044 .

Milutinović, Violeta, Petrović, Predrag, Petković, Miloš, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites" in Chemistry and Biodiversity (2023):e202301044,
https://doi.org/10.1002/cbdv.202301044 . .

TY  - CONF
AU  - Mijalković, Jelena R.
AU  - Šekuljica, Nataša Ž.
AU  - Jakovetić Tanasković, Sonja M.
AU  - Petrović, Predrag M.
AU  - Balanč, Bojana D.
AU  - Đorđević, Verica B.
AU  - Knežević-Jugović, Zorica D.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6914
AB  - The potential of food biodiversity and its production streams to generate additional features for isolating high-value products has been underutilized. Even so, the recovery of valuable nutritional compounds like proteins from waste streams and by-products has been identified as a key strategy for enhancing production sustainability in order to open up new market potential. In accordance with those facts, the primary aim of this research was to utilize a relatively novel extraction technique, high-intensity ultrasound, in order to achieve a reduction in time, energy, and extraction solvent consumption while at the same time improving the extraction yield and nutritional value of extracted leaf proteins. For this purpose, an ultrasound probe system with a low frequency (20±0.2 kHz) was utilized to extract the white proteins from the pumpkin leaf biomass, and the effects of different sonication amplitudes (20, 30, 40, 50, 60 and 70%) and duration periods (0, 1, 3, 5, 7.5, 10, 12.5, 15, 17.5, and 20 min) on the yield, solubility and emulsifying qualities, antioxidant properties, and structural characteristics of proteins were studied. In the functional properties evaluated, leaf proteins isolated using ultrasound outperformed those extracted using the conventional extraction method, maceration. High-intensity ultrasound resulted in a slight but gradual decline in solubility with an increase in amplitude, but a significant increase in solubility in an acidic environment was observed with the decrease of cavitation periods. Ultrasound-extracted proteins exhibit nearly 12, 1.5, and 3-fold greater solubility compared to the maceration-extracted sample at pH 3, 4, and 5, respectively. The emulsifying activity diminishes with increasing sonication amplitude and duration but increases in emulsifying stability as sonication periods are extended. The ultrasound extraction provided pumpkin proteins with high radical scavenging activities (i.e., good electron donors) and chelating activity, with half maximal inhibitory concentrations (IC50) in the range of 0.9 to 1.5 mg/ml, and 0.3 to 0.6, respectively, especially at 20 and 40% amplitude. Raman spectroscopy, surface charge, surface hydrophobicity, and sulfhydryl group contents were employed to characterize the structural changes brought on by ultrasound cavitation, and the achieved changes were more influenced by the treatment periods and amplitudes  applied. The experimental findings show that the use of ultrasound-emerging technology for protein extraction can significantly increase the yield of pumpkin leaf protein by up to 70%. The utilization of leaf proteins in food products and dietary supplements can be augmented by combining the ultrasound periods and amplitudes to create high-value samples with better capabilities.
PB  - Zvornik : Faculty of Technology
C3  - Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023
T1  - Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities
SP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6914
ER  - 

@conference{
author = "Mijalković, Jelena R. and Šekuljica, Nataša Ž. and Jakovetić Tanasković, Sonja M. and Petrović, Predrag M. and Balanč, Bojana D. and Đorđević, Verica B. and Knežević-Jugović, Zorica D.",
year = "2023",
abstract = "The potential of food biodiversity and its production streams to generate additional features for isolating high-value products has been underutilized. Even so, the recovery of valuable nutritional compounds like proteins from waste streams and by-products has been identified as a key strategy for enhancing production sustainability in order to open up new market potential. In accordance with those facts, the primary aim of this research was to utilize a relatively novel extraction technique, high-intensity ultrasound, in order to achieve a reduction in time, energy, and extraction solvent consumption while at the same time improving the extraction yield and nutritional value of extracted leaf proteins. For this purpose, an ultrasound probe system with a low frequency (20±0.2 kHz) was utilized to extract the white proteins from the pumpkin leaf biomass, and the effects of different sonication amplitudes (20, 30, 40, 50, 60 and 70%) and duration periods (0, 1, 3, 5, 7.5, 10, 12.5, 15, 17.5, and 20 min) on the yield, solubility and emulsifying qualities, antioxidant properties, and structural characteristics of proteins were studied. In the functional properties evaluated, leaf proteins isolated using ultrasound outperformed those extracted using the conventional extraction method, maceration. High-intensity ultrasound resulted in a slight but gradual decline in solubility with an increase in amplitude, but a significant increase in solubility in an acidic environment was observed with the decrease of cavitation periods. Ultrasound-extracted proteins exhibit nearly 12, 1.5, and 3-fold greater solubility compared to the maceration-extracted sample at pH 3, 4, and 5, respectively. The emulsifying activity diminishes with increasing sonication amplitude and duration but increases in emulsifying stability as sonication periods are extended. The ultrasound extraction provided pumpkin proteins with high radical scavenging activities (i.e., good electron donors) and chelating activity, with half maximal inhibitory concentrations (IC50) in the range of 0.9 to 1.5 mg/ml, and 0.3 to 0.6, respectively, especially at 20 and 40% amplitude. Raman spectroscopy, surface charge, surface hydrophobicity, and sulfhydryl group contents were employed to characterize the structural changes brought on by ultrasound cavitation, and the achieved changes were more influenced by the treatment periods and amplitudes  applied. The experimental findings show that the use of ultrasound-emerging technology for protein extraction can significantly increase the yield of pumpkin leaf protein by up to 70%. The utilization of leaf proteins in food products and dietary supplements can be augmented by combining the ultrasound periods and amplitudes to create high-value samples with better capabilities.",
publisher = "Zvornik : Faculty of Technology",
journal = "Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023",
title = "Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities",
pages = "178",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6914"
}

Mijalković, J. R., Šekuljica, N. Ž., Jakovetić Tanasković, S. M., Petrović, P. M., Balanč, B. D., Đorđević, V. B.,& Knežević-Jugović, Z. D.. (2023). Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities. in Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023
Zvornik : Faculty of Technology., 178.
https://hdl.handle.net/21.15107/rcub_technorep_6914

Mijalković JR, Šekuljica NŽ, Jakovetić Tanasković SM, Petrović PM, Balanč BD, Đorđević VB, Knežević-Jugović ZD. Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities. in Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023. 2023;:178.
https://hdl.handle.net/21.15107/rcub_technorep_6914 .

Mijalković, Jelena R., Šekuljica, Nataša Ž., Jakovetić Tanasković, Sonja M., Petrović, Predrag M., Balanč, Bojana D., Đorđević, Verica B., Knežević-Jugović, Zorica D., "Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities" in Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023 (2023):178,
https://hdl.handle.net/21.15107/rcub_technorep_6914 .

TY  - CONF
AU  - Pavlović, Neda
AU  - Mijalković, Jelena
AU  - Šekuljica, Nataša
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6251
AB  - Nowadays, the necessity for dietary nutrient encapsulation emerges largely since the action of digestive enzymes causes the loss of structural integrity and function of nutrients. Proteins have high nutritional value and thus are considered as the most nutritionally beneficial carriers; moreover, due to their versatility, proteins can be generated in a variety of structures (nanoparticles, nanofilms, nanofibers, coacervates, and hydrogels), allowing for the delivery of hydrophobic or hydrophilic nutrients. Proteins of vegetable origin have broader application potential over proteins of animal sources. Thus, the modern research focuses on waste leaf biomass, such as pumpkin leaves, as a rich source of plant proteins, specifically the most abundant protein in the world, ribulose-1,5-bisphosphate carboxylase-oxygenase, RuBisCo protein. 
The aim of this work was to isolate the water-soluble proteins, mostly RuBisCO protein, from pumpkin leaves in order to utilize it as a carrier for folic acid (FA) encapsulation. The resulting protein leaf concentrate was lyophilized and used as such to form nanoparticles. Leaf protein nanoparticles were prepared by employing a Ca-induced cold gelation method. 
Results and conclusions. The results showed that the Ca-induced protein nanoparticles have a small particle size (58–208 nm), polydispersity index (PDI) of 0.241 to 0.377, and negative zeta potential (-16.2 to -23.9 mV). Protein leaf nanoparticles exhibited uniform unimodal size distribution and spherical shape with a unique honeycomb-like core structure. Nanoparticle characteristics including size, surface charge and hydrophobicity could be adjustable by changing calcium chloride concentration from 2 to 10 mM and environmental pH (7 to 9). All manufactured nanoparticles demonstrated good stability over seven days, with no significant changes in zeta potential or formation agglomerates. Release studies showed that the leaf protein nanoparticles with FA, encapsulation efficiency amounted 40.2%, were resistant to pepsin and low pH in simulated gastric fluid (10% of initial FA amount was realized), but released the encapsulated FA in simulated intestinal conditions was enriched, even another 50% was achieved.  
Pumpkin leaf protein nanoparticles have a promising possibility for bioactive ingredient delivery owing to the characteristics described above. These findings would be of great importance for the development of food-grade nanoparticles suitable for the formulations of functional foods.
PB  - Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists
C3  - Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity
T1  - Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins
EP  - 142
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6251
ER  - 

@conference{
author = "Pavlović, Neda and Mijalković, Jelena and Šekuljica, Nataša and Petrović, Predrag and Đorđević, Verica and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "Nowadays, the necessity for dietary nutrient encapsulation emerges largely since the action of digestive enzymes causes the loss of structural integrity and function of nutrients. Proteins have high nutritional value and thus are considered as the most nutritionally beneficial carriers; moreover, due to their versatility, proteins can be generated in a variety of structures (nanoparticles, nanofilms, nanofibers, coacervates, and hydrogels), allowing for the delivery of hydrophobic or hydrophilic nutrients. Proteins of vegetable origin have broader application potential over proteins of animal sources. Thus, the modern research focuses on waste leaf biomass, such as pumpkin leaves, as a rich source of plant proteins, specifically the most abundant protein in the world, ribulose-1,5-bisphosphate carboxylase-oxygenase, RuBisCo protein. 
The aim of this work was to isolate the water-soluble proteins, mostly RuBisCO protein, from pumpkin leaves in order to utilize it as a carrier for folic acid (FA) encapsulation. The resulting protein leaf concentrate was lyophilized and used as such to form nanoparticles. Leaf protein nanoparticles were prepared by employing a Ca-induced cold gelation method. 
Results and conclusions. The results showed that the Ca-induced protein nanoparticles have a small particle size (58–208 nm), polydispersity index (PDI) of 0.241 to 0.377, and negative zeta potential (-16.2 to -23.9 mV). Protein leaf nanoparticles exhibited uniform unimodal size distribution and spherical shape with a unique honeycomb-like core structure. Nanoparticle characteristics including size, surface charge and hydrophobicity could be adjustable by changing calcium chloride concentration from 2 to 10 mM and environmental pH (7 to 9). All manufactured nanoparticles demonstrated good stability over seven days, with no significant changes in zeta potential or formation agglomerates. Release studies showed that the leaf protein nanoparticles with FA, encapsulation efficiency amounted 40.2%, were resistant to pepsin and low pH in simulated gastric fluid (10% of initial FA amount was realized), but released the encapsulated FA in simulated intestinal conditions was enriched, even another 50% was achieved.  
Pumpkin leaf protein nanoparticles have a promising possibility for bioactive ingredient delivery owing to the characteristics described above. These findings would be of great importance for the development of food-grade nanoparticles suitable for the formulations of functional foods.",
publisher = "Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists",
journal = "Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity",
title = "Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins",
pages = "142-141",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6251"
}

Pavlović, N., Mijalković, J., Šekuljica, N., Petrović, P., Đorđević, V., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins. in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity
Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists., 141-142.
https://hdl.handle.net/21.15107/rcub_technorep_6251

Pavlović N, Mijalković J, Šekuljica N, Petrović P, Đorđević V, Bugarski B, Knežević-Jugović Z. Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins. in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity. 2022;:141-142.
https://hdl.handle.net/21.15107/rcub_technorep_6251 .

Pavlović, Neda, Mijalković, Jelena, Šekuljica, Nataša, Petrović, Predrag, Đorđević, Verica, Bugarski, Branko, Knežević-Jugović, Zorica, "Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins" in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity (2022):141-142,
https://hdl.handle.net/21.15107/rcub_technorep_6251 .

TY  - CONF
AU  - Ugrinović, Vukašin
AU  - Hristara, Veroniki
AU  - Marković, Maja
AU  - Petrović, Predrag
AU  - Petrović, Rada
AU  - Janaćković, Đorđe
AU  - Veljović, Đorđe
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5240
AB  - Hydrogels are three-dimensional hydrophilic networks of polymers capable of retaining large
amounts of water or biological fluids, which makes them attractive for biomedical and
pharmaceutical applications. Although various types of polymers have been investigated as
hydrogels for biomedical purposes, those obtained from natural polymers have intrinsic
advantages as they are abundant, cheap, biocompatible and biodegradable. Gelatin is a natural
polymer that has been applied in biomedicine due to its low price, biocompatibility, and
biodegradability. HPMC, a derivative of cellulose, is a hydrophilic, biodegradable, and
biocompatible polymer. However, natural polymer-based hydrogels have low mechanical
properties and are relatively soluble in physiological conditions, which requires creative crosslinking strategies to improve the functionality of the hydrogels. The citric acid (CA) is an
inexpensive and non-toxic compound that has been proven to be an effective crosslinker for
natural polymers. In addition, the incorporation of bioactive calcium phosphate particles could
further improve mechanical properties and add new functionalities to the hydrogels.
In this work, we present novel composite hydrogels for biomedical applications, based on CAcrosslinked gelatin/HPMC (HPMC-G) matrix and Mg-doped biphasic calcium phosphate filler
(BCP). Firstly, the HPMC-G hydrogels crosslinking conditions were optimized. Different CA
concentrations, curing temperatures (140-180°C) and times (3-9 min) were applied. During the
second step, the optimaly crosslinked HPMC-G hydrogel was incorporated with different amounts
of BCP. BCP was obtained by calcination of Mg-doped hydroxyapatite (HA) powder synthesized
by the hydrothermal method. XRD analysis confirmed that powder was constituted only of HA
and beta-tricalcium phosphate. The obtained composite hydrogels were characterized by
mechanical testing, FTIR, SEM, swelling behavior and drug deliverability.
PB  - Materials Research Society of Serbia, Belgrade, Serbia
C3  - TWENTY THIRD ANNUAL CONFERENCE - YUCOMAT 2022, Herceg Novi, Montenegro
T1  - Composite hydrogels based on gelatin, hydroxypropyl methylcellulose and Mg-doped biphasic calcium phosphate for biomedical applications
UR  - https://hdl.handle.net/21.15107/rcub_technorep_5240
ER  - 

@conference{
author = "Ugrinović, Vukašin and Hristara, Veroniki and Marković, Maja and Petrović, Predrag and Petrović, Rada and Janaćković, Đorđe and Veljović, Đorđe",
year = "2022",
abstract = "Hydrogels are three-dimensional hydrophilic networks of polymers capable of retaining large
amounts of water or biological fluids, which makes them attractive for biomedical and
pharmaceutical applications. Although various types of polymers have been investigated as
hydrogels for biomedical purposes, those obtained from natural polymers have intrinsic
advantages as they are abundant, cheap, biocompatible and biodegradable. Gelatin is a natural
polymer that has been applied in biomedicine due to its low price, biocompatibility, and
biodegradability. HPMC, a derivative of cellulose, is a hydrophilic, biodegradable, and
biocompatible polymer. However, natural polymer-based hydrogels have low mechanical
properties and are relatively soluble in physiological conditions, which requires creative crosslinking strategies to improve the functionality of the hydrogels. The citric acid (CA) is an
inexpensive and non-toxic compound that has been proven to be an effective crosslinker for
natural polymers. In addition, the incorporation of bioactive calcium phosphate particles could
further improve mechanical properties and add new functionalities to the hydrogels.
In this work, we present novel composite hydrogels for biomedical applications, based on CAcrosslinked gelatin/HPMC (HPMC-G) matrix and Mg-doped biphasic calcium phosphate filler
(BCP). Firstly, the HPMC-G hydrogels crosslinking conditions were optimized. Different CA
concentrations, curing temperatures (140-180°C) and times (3-9 min) were applied. During the
second step, the optimaly crosslinked HPMC-G hydrogel was incorporated with different amounts
of BCP. BCP was obtained by calcination of Mg-doped hydroxyapatite (HA) powder synthesized
by the hydrothermal method. XRD analysis confirmed that powder was constituted only of HA
and beta-tricalcium phosphate. The obtained composite hydrogels were characterized by
mechanical testing, FTIR, SEM, swelling behavior and drug deliverability.",
publisher = "Materials Research Society of Serbia, Belgrade, Serbia",
journal = "TWENTY THIRD ANNUAL CONFERENCE - YUCOMAT 2022, Herceg Novi, Montenegro",
title = "Composite hydrogels based on gelatin, hydroxypropyl methylcellulose and Mg-doped biphasic calcium phosphate for biomedical applications",
url = "https://hdl.handle.net/21.15107/rcub_technorep_5240"
}

Ugrinović, V., Hristara, V., Marković, M., Petrović, P., Petrović, R., Janaćković, Đ.,& Veljović, Đ.. (2022). Composite hydrogels based on gelatin, hydroxypropyl methylcellulose and Mg-doped biphasic calcium phosphate for biomedical applications. in TWENTY THIRD ANNUAL CONFERENCE - YUCOMAT 2022, Herceg Novi, Montenegro
Materials Research Society of Serbia, Belgrade, Serbia..
https://hdl.handle.net/21.15107/rcub_technorep_5240

Ugrinović V, Hristara V, Marković M, Petrović P, Petrović R, Janaćković Đ, Veljović Đ. Composite hydrogels based on gelatin, hydroxypropyl methylcellulose and Mg-doped biphasic calcium phosphate for biomedical applications. in TWENTY THIRD ANNUAL CONFERENCE - YUCOMAT 2022, Herceg Novi, Montenegro. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_5240 .

Ugrinović, Vukašin, Hristara, Veroniki, Marković, Maja, Petrović, Predrag, Petrović, Rada, Janaćković, Đorđe, Veljović, Đorđe, "Composite hydrogels based on gelatin, hydroxypropyl methylcellulose and Mg-doped biphasic calcium phosphate for biomedical applications" in TWENTY THIRD ANNUAL CONFERENCE - YUCOMAT 2022, Herceg Novi, Montenegro (2022),
https://hdl.handle.net/21.15107/rcub_technorep_5240 .

TY  - JOUR
AU  - Šeremet, Danijela
AU  - Vugrinec, Kristina
AU  - Petrović, Predrag
AU  - Butorac, Ana
AU  - Kuzmić, Sunčica
AU  - Vojvodić Cebin, Aleksandra
AU  - Mandura, Ana
AU  - Lovrić, Marija
AU  - Pjanović, Rada
AU  - Komes, Draženka
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4987
AB  - Conventional and innovative (microwave-assisted and subcritical water extraction) techniques were applied to investigate the bioactive content of traditional plant - Teucrium montanum. Verbascoside and echinacoside, identified and quantified using LC-MS/MS and HPLC-PAD, were found to be the predominant phenolics in all extracts. Infusion (30 degrees C, 30 min) was characterized with the highest total phenolic content and antioxidant capacity and was further used for encapsulation into liposomes. Formulation of liposomes with a high encapsulation efficiency of echinacoside (68.27%) and verbascoside (80.60%), satisfactory physical properties, including size (326.2 nm) and polydispersity index (0.34), was achieved, although determined zeta potential (-23.03 mV) indicated their instability. Formulated liposomes were successfully coated with pectin and alginate that was also proved by FTIR analysis. Liposomes coated with pectin showed the most desirable in vitro digestion release of verbascoside and echinacoside, while alginate as liposome surface layer proved to be more appropriate for their retention during storage time.
PB  - Elsevier Ltd.
T2  - Food Chemistry
T1  - Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks
SP  - 131257
VL  - 370
DO  - 10.1016/j.foodchem.2021.131257
ER  - 

@article{
author = "Šeremet, Danijela and Vugrinec, Kristina and Petrović, Predrag and Butorac, Ana and Kuzmić, Sunčica and Vojvodić Cebin, Aleksandra and Mandura, Ana and Lovrić, Marija and Pjanović, Rada and Komes, Draženka",
year = "2022",
abstract = "Conventional and innovative (microwave-assisted and subcritical water extraction) techniques were applied to investigate the bioactive content of traditional plant - Teucrium montanum. Verbascoside and echinacoside, identified and quantified using LC-MS/MS and HPLC-PAD, were found to be the predominant phenolics in all extracts. Infusion (30 degrees C, 30 min) was characterized with the highest total phenolic content and antioxidant capacity and was further used for encapsulation into liposomes. Formulation of liposomes with a high encapsulation efficiency of echinacoside (68.27%) and verbascoside (80.60%), satisfactory physical properties, including size (326.2 nm) and polydispersity index (0.34), was achieved, although determined zeta potential (-23.03 mV) indicated their instability. Formulated liposomes were successfully coated with pectin and alginate that was also proved by FTIR analysis. Liposomes coated with pectin showed the most desirable in vitro digestion release of verbascoside and echinacoside, while alginate as liposome surface layer proved to be more appropriate for their retention during storage time.",
publisher = "Elsevier Ltd.",
journal = "Food Chemistry",
title = "Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks",
pages = "131257",
volume = "370",
doi = "10.1016/j.foodchem.2021.131257"
}

Šeremet, D., Vugrinec, K., Petrović, P., Butorac, A., Kuzmić, S., Vojvodić Cebin, A., Mandura, A., Lovrić, M., Pjanović, R.,& Komes, D.. (2022). Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks. in Food Chemistry
Elsevier Ltd.., 370, 131257.
https://doi.org/10.1016/j.foodchem.2021.131257

Šeremet D, Vugrinec K, Petrović P, Butorac A, Kuzmić S, Vojvodić Cebin A, Mandura A, Lovrić M, Pjanović R, Komes D. Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks. in Food Chemistry. 2022;370:131257.
doi:10.1016/j.foodchem.2021.131257 .

Šeremet, Danijela, Vugrinec, Kristina, Petrović, Predrag, Butorac, Ana, Kuzmić, Sunčica, Vojvodić Cebin, Aleksandra, Mandura, Ana, Lovrić, Marija, Pjanović, Rada, Komes, Draženka, "Formulation and characterization of liposomal encapsulated systems of bioactive ingredients from traditional plant mountain germander (Teucrium montanum L.) for the incorporation into coffee drinks" in Food Chemistry, 370 (2022):131257,
https://doi.org/10.1016/j.foodchem.2021.131257 . .

TY  - JOUR
AU  - Šeremet, Danijela
AU  - Štefančić, Martina
AU  - Petrović, Predrag
AU  - Kuzmić, Sunčica
AU  - Doroci, Shefkije
AU  - Mandura Jarić, Ana
AU  - Vojvodić Cebin, Aleksandra
AU  - Pjanović, Rada
AU  - Komes, Draženka
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5327
AB  - Ground ivy (Glechoma hederacea L.) has been known as a medicinal plant in folk medicine for generations and, as a member of the Lamiaceae family, is characterized with a high content of rosmarinic acid. The aim of the present study was to formulate delivery systems containing bioac-tive compounds from ground ivy in encapsulated form and incorporated into candies. Liposomes were examined as the encapsulation systems that were additionally coated with an alginate–plant protein gel to reduce leakage of the incorporated material. Bioactive characterization of the ground ivy extract showed a high content of total phenolics (1186.20 mg GAE/L) and rosmarinic acid (46.04 mg/L). The formulation of liposomes with the high encapsulation efficiency of rosmarinic acid (97.64%), with at least a double bilayer and with polydisperse particle size distribution was achieved. Alginate microparticles reinforced with rice proteins provided the highest encapsulation efficiency for rosmarinic acid (78.16%) and were therefore used for the successful coating of lipo-somes, as confirmed by FT‐IR analysis. Coating liposomes with alginate–rice protein gel provided prolonged controlled release of rosmarinic acid during simulated gastro‐intestinal digestion, and the same was noted when they were incorporated into candies.
PB  - MDPI
T2  - Foods
T1  - Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies
IS  - 12
SP  - 1816
VL  - 11
DO  - 10.3390/foods11121816
ER  - 

@article{
author = "Šeremet, Danijela and Štefančić, Martina and Petrović, Predrag and Kuzmić, Sunčica and Doroci, Shefkije and Mandura Jarić, Ana and Vojvodić Cebin, Aleksandra and Pjanović, Rada and Komes, Draženka",
year = "2022",
abstract = "Ground ivy (Glechoma hederacea L.) has been known as a medicinal plant in folk medicine for generations and, as a member of the Lamiaceae family, is characterized with a high content of rosmarinic acid. The aim of the present study was to formulate delivery systems containing bioac-tive compounds from ground ivy in encapsulated form and incorporated into candies. Liposomes were examined as the encapsulation systems that were additionally coated with an alginate–plant protein gel to reduce leakage of the incorporated material. Bioactive characterization of the ground ivy extract showed a high content of total phenolics (1186.20 mg GAE/L) and rosmarinic acid (46.04 mg/L). The formulation of liposomes with the high encapsulation efficiency of rosmarinic acid (97.64%), with at least a double bilayer and with polydisperse particle size distribution was achieved. Alginate microparticles reinforced with rice proteins provided the highest encapsulation efficiency for rosmarinic acid (78.16%) and were therefore used for the successful coating of lipo-somes, as confirmed by FT‐IR analysis. Coating liposomes with alginate–rice protein gel provided prolonged controlled release of rosmarinic acid during simulated gastro‐intestinal digestion, and the same was noted when they were incorporated into candies.",
publisher = "MDPI",
journal = "Foods",
title = "Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies",
number = "12",
pages = "1816",
volume = "11",
doi = "10.3390/foods11121816"
}

Šeremet, D., Štefančić, M., Petrović, P., Kuzmić, S., Doroci, S., Mandura Jarić, A., Vojvodić Cebin, A., Pjanović, R.,& Komes, D.. (2022). Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies. in Foods
MDPI., 11(12), 1816.
https://doi.org/10.3390/foods11121816

Šeremet D, Štefančić M, Petrović P, Kuzmić S, Doroci S, Mandura Jarić A, Vojvodić Cebin A, Pjanović R, Komes D. Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies. in Foods. 2022;11(12):1816.
doi:10.3390/foods11121816 .

Šeremet, Danijela, Štefančić, Martina, Petrović, Predrag, Kuzmić, Sunčica, Doroci, Shefkije, Mandura Jarić, Ana, Vojvodić Cebin, Aleksandra, Pjanović, Rada, Komes, Draženka, "Development, Characterization and Incorporation of Alginate‐Plant Protein Covered Liposomes Containing Ground Ivy (Glechoma hederacea L.) Extract into Candies" in Foods, 11, no. 12 (2022):1816,
https://doi.org/10.3390/foods11121816 . .

TY  - CHAP
AU  - Petrović, Predrag
AU  - Vunduk, Jovana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6138
AB  - Mushrooms have been used in traditional medicine for centuries, although only relatively recently they have become a subject of intensive studies as a source of potential drugs and products that could be used in the treatment of many disorders. Mushroom-forming fungi are shown to produce a vast number of unique metabolites that exhibit various biological effects; these include polysaccharides, terpenoids, polyketides, and amino compounds. The most studied use of mushrooms is for cancer treatment; mushrooms are a source of both compounds with direct antitumor effect, as well as immunomodulating polysaccharides (particularly β-glucans), which have been shown to stimulate the host's immune system and immunological response to cancer cells. Glucan-based mushroom products, such as lentinan from Lentinula edodes and PSK from Trametes versicolor, are clinically proven to be beneficial in the treatment of certain cancer types. Mushrooms are also known to be a source of potent cytotoxic compounds, such are illudins, clitocine, and ganoderic acids. As microbial resistance to antibiotics is becoming more and more prevalent, mushrooms are seen as a good source of new classes of compounds with antimicrobial activity, some of which, such as pleuromutilin, have led to the synthesis of new drugs that have been recently approved for use in humans. Psychedelic mushrooms and psilocybin have also been studied as breakthrough therapies for depression. Mushroom consumption has been associated with beneficial effects on sugar and lipid metabolism, which led to increased interest in research of mushroom product use in the treatment of metabolic disorders. Although mushrooms are often presumed to be a rich source of certain compounds with vitamin activity, this may not be the case. However, mushroom production can be manipulated to obtain higher yields of physiologically active compounds, such as vitamin D.
PB  - Taylor & Francis
T2  - WILD MUSHROOMS: Characteristics, Nutrition, and Processing
T1  - Nature and chemistry of bioactive components of wild edible mushrooms
EP  - 257
SP  - 211
DO  - 10.1201/9781003152583
ER  - 

@inbook{
author = "Petrović, Predrag and Vunduk, Jovana",
year = "2022",
abstract = "Mushrooms have been used in traditional medicine for centuries, although only relatively recently they have become a subject of intensive studies as a source of potential drugs and products that could be used in the treatment of many disorders. Mushroom-forming fungi are shown to produce a vast number of unique metabolites that exhibit various biological effects; these include polysaccharides, terpenoids, polyketides, and amino compounds. The most studied use of mushrooms is for cancer treatment; mushrooms are a source of both compounds with direct antitumor effect, as well as immunomodulating polysaccharides (particularly β-glucans), which have been shown to stimulate the host's immune system and immunological response to cancer cells. Glucan-based mushroom products, such as lentinan from Lentinula edodes and PSK from Trametes versicolor, are clinically proven to be beneficial in the treatment of certain cancer types. Mushrooms are also known to be a source of potent cytotoxic compounds, such are illudins, clitocine, and ganoderic acids. As microbial resistance to antibiotics is becoming more and more prevalent, mushrooms are seen as a good source of new classes of compounds with antimicrobial activity, some of which, such as pleuromutilin, have led to the synthesis of new drugs that have been recently approved for use in humans. Psychedelic mushrooms and psilocybin have also been studied as breakthrough therapies for depression. Mushroom consumption has been associated with beneficial effects on sugar and lipid metabolism, which led to increased interest in research of mushroom product use in the treatment of metabolic disorders. Although mushrooms are often presumed to be a rich source of certain compounds with vitamin activity, this may not be the case. However, mushroom production can be manipulated to obtain higher yields of physiologically active compounds, such as vitamin D.",
publisher = "Taylor & Francis",
journal = "WILD MUSHROOMS: Characteristics, Nutrition, and Processing",
booktitle = "Nature and chemistry of bioactive components of wild edible mushrooms",
pages = "257-211",
doi = "10.1201/9781003152583"
}

Petrović, P.,& Vunduk, J.. (2022). Nature and chemistry of bioactive components of wild edible mushrooms. in WILD MUSHROOMS: Characteristics, Nutrition, and Processing
Taylor & Francis., 211-257.
https://doi.org/10.1201/9781003152583

Petrović P, Vunduk J. Nature and chemistry of bioactive components of wild edible mushrooms. in WILD MUSHROOMS: Characteristics, Nutrition, and Processing. 2022;:211-257.
doi:10.1201/9781003152583 .

Petrović, Predrag, Vunduk, Jovana, "Nature and chemistry of bioactive components of wild edible mushrooms" in WILD MUSHROOMS: Characteristics, Nutrition, and Processing (2022):211-257,
https://doi.org/10.1201/9781003152583 . .

TY  - CHAP
AU  - Petrović, Predrag
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6144
AB  - Wild mushroom foraging has always come with the risk of mistaking edible mushrooms with poisonous ones. Poisonous mushrooms can cause a diverse spectrum of clinical signs and symptoms, ranging from simple, self-limited gastrointestinal upset to fatal multi-organ failure. Depending on the mechanism of action, affected organ(s), and clinical presentation, mushroom poisonings can be divided into several syndromes. Some mushroom poisoning syndromes are well characterized, but many are yet to be fully understood; some of the responsible toxins are known, but many are yet to be isolated. New poisonous mushrooms and new poisoning syndromes are being recognized in recent times, as well as the fact that mushroom toxicity is in many cases relative – there is a great inter-individual difference in susceptibility to some mushrooms toxins. Hepatotoxic mushrooms that contain amatoxins, mostly those of the genus Amanita, are still responsible for the great majority of mushroom poisoning-associated deaths worldwide. Other species can exert nephrotoxicity and lead to renal failure; more species however show neurotoxicity, while the greatest number of species primarily causes gastrointestinal disorders. Some species are still debated whether they are toxic or not. In the end, edible mushrooms can cause poisonings as well, if improperly handled, and are actually the most frequently reported cause of mushroom poisonings.
PB  - Taylor & Francis
T2  - WILD MUSHROOMS: Characteristics, Nutrition, and Processing
T1  - Toxic components and toxicology of wild mushrooms
EP  - 413
SP  - 369
DO  - 10.1201/9781003152583
ER  - 

@inbook{
author = "Petrović, Predrag",
year = "2022",
abstract = "Wild mushroom foraging has always come with the risk of mistaking edible mushrooms with poisonous ones. Poisonous mushrooms can cause a diverse spectrum of clinical signs and symptoms, ranging from simple, self-limited gastrointestinal upset to fatal multi-organ failure. Depending on the mechanism of action, affected organ(s), and clinical presentation, mushroom poisonings can be divided into several syndromes. Some mushroom poisoning syndromes are well characterized, but many are yet to be fully understood; some of the responsible toxins are known, but many are yet to be isolated. New poisonous mushrooms and new poisoning syndromes are being recognized in recent times, as well as the fact that mushroom toxicity is in many cases relative – there is a great inter-individual difference in susceptibility to some mushrooms toxins. Hepatotoxic mushrooms that contain amatoxins, mostly those of the genus Amanita, are still responsible for the great majority of mushroom poisoning-associated deaths worldwide. Other species can exert nephrotoxicity and lead to renal failure; more species however show neurotoxicity, while the greatest number of species primarily causes gastrointestinal disorders. Some species are still debated whether they are toxic or not. In the end, edible mushrooms can cause poisonings as well, if improperly handled, and are actually the most frequently reported cause of mushroom poisonings.",
publisher = "Taylor & Francis",
journal = "WILD MUSHROOMS: Characteristics, Nutrition, and Processing",
booktitle = "Toxic components and toxicology of wild mushrooms",
pages = "413-369",
doi = "10.1201/9781003152583"
}

Petrović, P.. (2022). Toxic components and toxicology of wild mushrooms. in WILD MUSHROOMS: Characteristics, Nutrition, and Processing
Taylor & Francis., 369-413.
https://doi.org/10.1201/9781003152583

Petrović P. Toxic components and toxicology of wild mushrooms. in WILD MUSHROOMS: Characteristics, Nutrition, and Processing. 2022;:369-413.
doi:10.1201/9781003152583 .

Petrović, Predrag, "Toxic components and toxicology of wild mushrooms" in WILD MUSHROOMS: Characteristics, Nutrition, and Processing (2022):369-413,
https://doi.org/10.1201/9781003152583 . .

TY  - JOUR
AU  - Milosevic, Dragana
AU  - Levic, Steva
AU  - Lazarević, Slavica
AU  - Veličković, Zlate
AU  - Marinković, Aleksandar
AU  - Petrović, Rada
AU  - Petrović, Predrag
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4788
AB  - The alkali treated subglebal tissue of the mosaic puffball (Handkea utriformis) (Sa) and Sa modified with hydroxyapatite (Sa-HAp), obtained by successive ionic layer adsorption and reaction (SILAR) method, were used for the removal of Pb2+, Cd2+ and Ni2+ from aqueous solution. The materials were characterized by FT-IR, Raman, SEM and EDS analysis and by determination of pH(PZC). The adsorption performances of Sa and SaHAp were assessed in batch experiments at different pH, contact times, temperatures and mass of the adsorbent. Different models of adsorption isotherms were used, and the best fit was obtained with the Langmuir model. Maximum adsorption capacities of Sa towards Pb2+, Cd2+ and Ni2+ were 44.82, 15.54 and 17.21 mg g(-1), while for Sa-HAp were 79.55, 52.59 and 45.01 mg g(-1), respectively. Kinetic data were well fitted by a pseudo second-order model, while thermodynamic studies disclose spontaneous and endothermic adsorption process. The Sa-Hap was successfully regenerated with 1 M NaCl and after the fifth desorption cycle and 10 h achieved 82.9, 69.7 and 60.4 %, while for 0.5 M NaCl + 0.5 M NaOH and 1 h was 78.3, 64.1, 57.5 % of desorbed Pb2+, Cd2+ and Ni2+, respectively. The competitive study and results from a column system confirmed good applicability of Sa-HAp adsorbent.
T2  - Journal of Environmental Management
T1  - Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions
VL  - 297
DO  - 10.1016/j.jenvman.2021.113358
ER  - 

@article{
author = "Milosevic, Dragana and Levic, Steva and Lazarević, Slavica and Veličković, Zlate and Marinković, Aleksandar and Petrović, Rada and Petrović, Predrag",
year = "2021",
abstract = "The alkali treated subglebal tissue of the mosaic puffball (Handkea utriformis) (Sa) and Sa modified with hydroxyapatite (Sa-HAp), obtained by successive ionic layer adsorption and reaction (SILAR) method, were used for the removal of Pb2+, Cd2+ and Ni2+ from aqueous solution. The materials were characterized by FT-IR, Raman, SEM and EDS analysis and by determination of pH(PZC). The adsorption performances of Sa and SaHAp were assessed in batch experiments at different pH, contact times, temperatures and mass of the adsorbent. Different models of adsorption isotherms were used, and the best fit was obtained with the Langmuir model. Maximum adsorption capacities of Sa towards Pb2+, Cd2+ and Ni2+ were 44.82, 15.54 and 17.21 mg g(-1), while for Sa-HAp were 79.55, 52.59 and 45.01 mg g(-1), respectively. Kinetic data were well fitted by a pseudo second-order model, while thermodynamic studies disclose spontaneous and endothermic adsorption process. The Sa-Hap was successfully regenerated with 1 M NaCl and after the fifth desorption cycle and 10 h achieved 82.9, 69.7 and 60.4 %, while for 0.5 M NaCl + 0.5 M NaOH and 1 h was 78.3, 64.1, 57.5 % of desorbed Pb2+, Cd2+ and Ni2+, respectively. The competitive study and results from a column system confirmed good applicability of Sa-HAp adsorbent.",
journal = "Journal of Environmental Management",
title = "Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions",
volume = "297",
doi = "10.1016/j.jenvman.2021.113358"
}

Milosevic, D., Levic, S., Lazarević, S., Veličković, Z., Marinković, A., Petrović, R.,& Petrović, P.. (2021). Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions. in Journal of Environmental Management, 297.
https://doi.org/10.1016/j.jenvman.2021.113358

Milosevic D, Levic S, Lazarević S, Veličković Z, Marinković A, Petrović R, Petrović P. Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions. in Journal of Environmental Management. 2021;297.
doi:10.1016/j.jenvman.2021.113358 .

Milosevic, Dragana, Levic, Steva, Lazarević, Slavica, Veličković, Zlate, Marinković, Aleksandar, Petrović, Rada, Petrović, Predrag, "Hybrid material based on subgleba of mosaic puffball mushroom (Handkea utriformis) as an adsorbent for heavy metal removal from aqueous solutions" in Journal of Environmental Management, 297 (2021),
https://doi.org/10.1016/j.jenvman.2021.113358 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Petrović, Predrag
AU  - Pljevljakusic, Dejan S.
AU  - Zdunic, Gordana M.
AU  - Savikin, Katarina P.
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4794
AB  - The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme
VL  - 25
DO  - 10.1016/j.jarmap.2021.100328
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Petrović, Predrag and Pljevljakusic, Dejan S. and Zdunic, Gordana M. and Savikin, Katarina P. and Bugarski, Branko",
year = "2021",
abstract = "The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme",
volume = "25",
doi = "10.1016/j.jarmap.2021.100328"
}

Jovanović, A., Đorđević, V., Petrović, P., Pljevljakusic, D. S., Zdunic, G. M., Savikin, K. P.,& Bugarski, B.. (2021). The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants, 25.
https://doi.org/10.1016/j.jarmap.2021.100328

Jovanović A, Đorđević V, Petrović P, Pljevljakusic DS, Zdunic GM, Savikin KP, Bugarski B. The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants. 2021;25.
doi:10.1016/j.jarmap.2021.100328 .

Jovanović, Aleksandra, Đorđević, Verica, Petrović, Predrag, Pljevljakusic, Dejan S., Zdunic, Gordana M., Savikin, Katarina P., Bugarski, Branko, "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme" in Journal of Applied Research on Medicinal and Aromatic Plants, 25 (2021),
https://doi.org/10.1016/j.jarmap.2021.100328 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Zdunic, Gordana M.
AU  - Savikin, Katarina P.
AU  - Kitic, Dusanka
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Brankovic, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4798
AB  - In the presented study, polyphenol profile, spasmolytic, antimicrobial and antioxidant properties of lyophilized Thymus serpyllum extracts were examined. The main phenolic acid in the extracts was rosmarinic acid, followed by salvianolic acid I, salvianolic acid K isomer and caffeic acid, whereas chlorogenic acid was detected only in traces; the dominant flavonoids were luteolin 7-O-glucuronide and 6-hydroxyluteolin 7-O-glucoside, while the contents of apigenin glucuronide and 6,8-di-C-glucosylapigenin were significantly lower. All tested extracts have shown spasmolytic effect in a dose-dependent manner on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions; lyophilized extract obtained in maceration has shown significantly better spasmolytic activity. The mentioned extract has shown dosedependent spasmolytic effect in the model of contractions induced by barium chloride and calcium chloride, as well. Furthermore, lyophilized extracts were effective against Gram-positive bacteria and showed only moderate antifungal activity. Ultrasound-assisted extraction provided extracts with better ability of neutralization DPPH radicals, whereas maceration and ultrasound-assisted extraction approved to be better regarding beta-carotene bleaching assay. Due to spasmolytic, antimicrobial and antioxidant properties shown in the present study, polyphenols-rich T. serpyllum extract may be used as adjuvant in therapy of gastrointestinal disorders and infections caused by some of the susceptible pathogens.
T2  - South African Journal of Botany
T1  - Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties
EP  - 283
SP  - 274
VL  - 142
DO  - 10.1016/j.sajb.2021.06.028
ER  - 

@article{
author = "Jovanović, Aleksandra and Petrović, Predrag and Zdunic, Gordana M. and Savikin, Katarina P. and Kitic, Dusanka and Đorđević, Verica and Bugarski, Branko and Brankovic, Suzana",
year = "2021",
abstract = "In the presented study, polyphenol profile, spasmolytic, antimicrobial and antioxidant properties of lyophilized Thymus serpyllum extracts were examined. The main phenolic acid in the extracts was rosmarinic acid, followed by salvianolic acid I, salvianolic acid K isomer and caffeic acid, whereas chlorogenic acid was detected only in traces; the dominant flavonoids were luteolin 7-O-glucuronide and 6-hydroxyluteolin 7-O-glucoside, while the contents of apigenin glucuronide and 6,8-di-C-glucosylapigenin were significantly lower. All tested extracts have shown spasmolytic effect in a dose-dependent manner on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions; lyophilized extract obtained in maceration has shown significantly better spasmolytic activity. The mentioned extract has shown dosedependent spasmolytic effect in the model of contractions induced by barium chloride and calcium chloride, as well. Furthermore, lyophilized extracts were effective against Gram-positive bacteria and showed only moderate antifungal activity. Ultrasound-assisted extraction provided extracts with better ability of neutralization DPPH radicals, whereas maceration and ultrasound-assisted extraction approved to be better regarding beta-carotene bleaching assay. Due to spasmolytic, antimicrobial and antioxidant properties shown in the present study, polyphenols-rich T. serpyllum extract may be used as adjuvant in therapy of gastrointestinal disorders and infections caused by some of the susceptible pathogens.",
journal = "South African Journal of Botany",
title = "Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties",
pages = "283-274",
volume = "142",
doi = "10.1016/j.sajb.2021.06.028"
}

Jovanović, A., Petrović, P., Zdunic, G. M., Savikin, K. P., Kitic, D., Đorđević, V., Bugarski, B.,& Brankovic, S.. (2021). Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties. in South African Journal of Botany, 142, 274-283.
https://doi.org/10.1016/j.sajb.2021.06.028

Jovanović A, Petrović P, Zdunic GM, Savikin KP, Kitic D, Đorđević V, Bugarski B, Brankovic S. Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties. in South African Journal of Botany. 2021;142:274-283.
doi:10.1016/j.sajb.2021.06.028 .

Jovanović, Aleksandra, Petrović, Predrag, Zdunic, Gordana M., Savikin, Katarina P., Kitic, Dusanka, Đorđević, Verica, Bugarski, Branko, Brankovic, Suzana, "Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties" in South African Journal of Botany, 142 (2021):274-283,
https://doi.org/10.1016/j.sajb.2021.06.028 . .

TY  - JOUR
AU  - Jovanovic, Milos
AU  - Cujic-Nikolic, Nada
AU  - Drinic, Zorica
AU  - Jankovic, Teodora
AU  - Markovic, Smilja
AU  - Petrović, Predrag
AU  - Savikin, Katarina
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4799
AB  - Willow gentian as a source of bitter compounds is traditionally used for digestive disorders. Gentiana root extract was spray dried using five different carriers (maltodextrin, whey protein, pectin, starch, gelatine) at various concentrations. Powders were characterized in terms of physical properties and encapsulation efficiency of bioactive compounds. The moisture content of all powders was between 1.78 and 3.46 %, and bulk density from 0.23 to 0.32 g/mL. Powders produced with maltodextrin and whey protein provided the highest yield (around 75 and 70 %, respectively) and the lowest hygroscopicity (6 and 7%, respectively). Gelatin and pectin provided powders with the highest encapsulation efficiency of total phenolic as well as the individual compounds. The stability of encapsulated bioactive compounds was studied after 6 months, and the most stable in all samples were gentiopicrin and sweroside with their content decreased by 10 % only. This study has shown that spray drying of gentian root extract produces powders with good physical properties and encapsulation of bioactives.
T2  - Industrial Crops and Products
T1  - Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds
VL  - 172
DO  - 10.1016/j.indcrop.2021.114044
ER  - 

@article{
author = "Jovanovic, Milos and Cujic-Nikolic, Nada and Drinic, Zorica and Jankovic, Teodora and Markovic, Smilja and Petrović, Predrag and Savikin, Katarina",
year = "2021",
abstract = "Willow gentian as a source of bitter compounds is traditionally used for digestive disorders. Gentiana root extract was spray dried using five different carriers (maltodextrin, whey protein, pectin, starch, gelatine) at various concentrations. Powders were characterized in terms of physical properties and encapsulation efficiency of bioactive compounds. The moisture content of all powders was between 1.78 and 3.46 %, and bulk density from 0.23 to 0.32 g/mL. Powders produced with maltodextrin and whey protein provided the highest yield (around 75 and 70 %, respectively) and the lowest hygroscopicity (6 and 7%, respectively). Gelatin and pectin provided powders with the highest encapsulation efficiency of total phenolic as well as the individual compounds. The stability of encapsulated bioactive compounds was studied after 6 months, and the most stable in all samples were gentiopicrin and sweroside with their content decreased by 10 % only. This study has shown that spray drying of gentian root extract produces powders with good physical properties and encapsulation of bioactives.",
journal = "Industrial Crops and Products",
title = "Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds",
volume = "172",
doi = "10.1016/j.indcrop.2021.114044"
}

Jovanovic, M., Cujic-Nikolic, N., Drinic, Z., Jankovic, T., Markovic, S., Petrović, P.,& Savikin, K.. (2021). Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds. in Industrial Crops and Products, 172.
https://doi.org/10.1016/j.indcrop.2021.114044

Jovanovic M, Cujic-Nikolic N, Drinic Z, Jankovic T, Markovic S, Petrović P, Savikin K. Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds. in Industrial Crops and Products. 2021;172.
doi:10.1016/j.indcrop.2021.114044 .

Jovanovic, Milos, Cujic-Nikolic, Nada, Drinic, Zorica, Jankovic, Teodora, Markovic, Smilja, Petrović, Predrag, Savikin, Katarina, "Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds" in Industrial Crops and Products, 172 (2021),
https://doi.org/10.1016/j.indcrop.2021.114044 . .

TY  - JOUR
AU  - Sabovljevic, Marko S.
AU  - Tomovic, Gordana
AU  - Boycheva, Petya
AU  - Ivanov, Dobri
AU  - Denchev, Teodor T.
AU  - Denchev, Cvetomir M.
AU  - Stevanoski, Ivana
AU  - Markovic, Aleksandra
AU  - Djurovic, Sanja Z.
AU  - Buzurovic, Uros
AU  - Yaneva, Galina
AU  - Stefanut, Sorin
AU  - Stefanut, Miruna-Maria
AU  - Knezevic, Aleksandar
AU  - Petrović, Predrag
AU  - Assyov, Boris
AU  - Pantovic, Jovana
AU  - Niketic, Marjan
AU  - Vukojicic, Snezana
AU  - Ion, Roxana
AU  - Tamas, Gabriela
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4882
AB  - This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: parasitic fungus Antherospora hortensis, saprotrophic fungi Loweomyces fractipes and Pholiota henningsii, stonewort Chara canescens, mosses Grimmia caespiticia and Rhodobryum ontariense, fern Woodsia alpina, monocots Aegilops triuncialis, Epipactis purpurata, Galanthus elwesii and Typha shuttleworthii and dicot Umbilicus luteus.
T2  - Botanica Serbica
T1  - New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3
EP  - 127
IS  - 1
SP  - 119
VL  - 45
DO  - 10.2298/BOTSERB2101119S
ER  - 

@article{
author = "Sabovljevic, Marko S. and Tomovic, Gordana and Boycheva, Petya and Ivanov, Dobri and Denchev, Teodor T. and Denchev, Cvetomir M. and Stevanoski, Ivana and Markovic, Aleksandra and Djurovic, Sanja Z. and Buzurovic, Uros and Yaneva, Galina and Stefanut, Sorin and Stefanut, Miruna-Maria and Knezevic, Aleksandar and Petrović, Predrag and Assyov, Boris and Pantovic, Jovana and Niketic, Marjan and Vukojicic, Snezana and Ion, Roxana and Tamas, Gabriela",
year = "2021",
abstract = "This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: parasitic fungus Antherospora hortensis, saprotrophic fungi Loweomyces fractipes and Pholiota henningsii, stonewort Chara canescens, mosses Grimmia caespiticia and Rhodobryum ontariense, fern Woodsia alpina, monocots Aegilops triuncialis, Epipactis purpurata, Galanthus elwesii and Typha shuttleworthii and dicot Umbilicus luteus.",
journal = "Botanica Serbica",
title = "New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3",
pages = "127-119",
number = "1",
volume = "45",
doi = "10.2298/BOTSERB2101119S"
}

Sabovljevic, M. S., Tomovic, G., Boycheva, P., Ivanov, D., Denchev, T. T., Denchev, C. M., Stevanoski, I., Markovic, A., Djurovic, S. Z., Buzurovic, U., Yaneva, G., Stefanut, S., Stefanut, M., Knezevic, A., Petrović, P., Assyov, B., Pantovic, J., Niketic, M., Vukojicic, S., Ion, R.,& Tamas, G.. (2021). New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3. in Botanica Serbica, 45(1), 119-127.
https://doi.org/10.2298/BOTSERB2101119S

Sabovljevic MS, Tomovic G, Boycheva P, Ivanov D, Denchev TT, Denchev CM, Stevanoski I, Markovic A, Djurovic SZ, Buzurovic U, Yaneva G, Stefanut S, Stefanut M, Knezevic A, Petrović P, Assyov B, Pantovic J, Niketic M, Vukojicic S, Ion R, Tamas G. New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3. in Botanica Serbica. 2021;45(1):119-127.
doi:10.2298/BOTSERB2101119S .

Sabovljevic, Marko S., Tomovic, Gordana, Boycheva, Petya, Ivanov, Dobri, Denchev, Teodor T., Denchev, Cvetomir M., Stevanoski, Ivana, Markovic, Aleksandra, Djurovic, Sanja Z., Buzurovic, Uros, Yaneva, Galina, Stefanut, Sorin, Stefanut, Miruna-Maria, Knezevic, Aleksandar, Petrović, Predrag, Assyov, Boris, Pantovic, Jovana, Niketic, Marjan, Vukojicic, Snezana, Ion, Roxana, Tamas, Gabriela, "New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 3" in Botanica Serbica, 45, no. 1 (2021):119-127,
https://doi.org/10.2298/BOTSERB2101119S . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana D.
AU  - Petrović, Predrag
AU  - Pravilović, Radoslava
AU  - Đorđević, Verica B.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5997
AB  - In traditional medicine Thymus serpyllum L. (wild thyme) herb represents a part of a large number of herbal medicinal formulations such as syrups, tinctures, infusions, teas, and decoctions. In recent years, there has been a growing interest in testing the biological properties of wild thyme, because the plant is a high-quality raw material, rich in essential oil and pharmacologically active polyphenolic compounds, which can be included in various formulations in the pharmaceutical, cosmetic, food, and chemical industries. Wild thyme extracts and essential oil have shown significant nitric oxide, ABTS and DPPH radicals scavenging potential, ferrous ion chelating property, reducing activity and ability to inhibit peroxidation of deoxyribonucleic acid (DNA), proteins and lipids. Wild thyme extracts exerted antibacterial activity against Bacillus cereus, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enterica, Yersinia enterocolitica, as well as Lactic acid bacteria. Wild thyme essential oil has shown antibacterial potential against E. coli, L. monocytogenes, P. aeruginosa, S. enteritidis, S. aureus, Streptococcus salivariu, S. mutans, S. sanguinis, S. pyogenes, E. feacalis, B. cereus, B. subtilis, B. pumilis, Lactobacillus acidophilus, Proteus mirabilis, Klebsiella pneumoniae, Salmonella choleraesuis, and Salmonella poona. T. serpyllum extract possesses antifungal activity against Fusarium solani, F. moniliforme, Aspergillus flavus, Microsporum canis, Alternaria species, Candida albicans, and C. glabarata. The essential oil efficiently inhibited the growth of C. albicans, C. glabrata and Aspergillus fungi. Depending on the concentration, wild thyme extract significantly relaxed spontaneous contractions, as well as acetylcholine-, potassium chloride-, barium chlorideand calcium chloride-induced contractions of the isolated rat ileum. The extract significantly reduced the expression of the inducible enzyme cyclooxygenase-2 and inhibited acetylcholinesterase, myeloperoxidase and α-glucosidase activity. T. serpyllum extract has shown cytotoxic activity on human breast cancer cell lines, while essential oil has shown the antitumor potential in human cell lung cancer, colon, cervical, hepatocellular, prostate, and breast adeno-carcinoma.
PB  - University of East Sarajevo, Faculty of Technology
T2  - Journal of Engineering & Processing Management
T1  - Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review
EP  - 41
IS  - 2
SP  - 32
VL  - 13
DO  - 10.7251/JEPM2102032J
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Balanč, Bojana D. and Petrović, Predrag and Pravilović, Radoslava and Đorđević, Verica B.",
year = "2021",
abstract = "In traditional medicine Thymus serpyllum L. (wild thyme) herb represents a part of a large number of herbal medicinal formulations such as syrups, tinctures, infusions, teas, and decoctions. In recent years, there has been a growing interest in testing the biological properties of wild thyme, because the plant is a high-quality raw material, rich in essential oil and pharmacologically active polyphenolic compounds, which can be included in various formulations in the pharmaceutical, cosmetic, food, and chemical industries. Wild thyme extracts and essential oil have shown significant nitric oxide, ABTS and DPPH radicals scavenging potential, ferrous ion chelating property, reducing activity and ability to inhibit peroxidation of deoxyribonucleic acid (DNA), proteins and lipids. Wild thyme extracts exerted antibacterial activity against Bacillus cereus, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enterica, Yersinia enterocolitica, as well as Lactic acid bacteria. Wild thyme essential oil has shown antibacterial potential against E. coli, L. monocytogenes, P. aeruginosa, S. enteritidis, S. aureus, Streptococcus salivariu, S. mutans, S. sanguinis, S. pyogenes, E. feacalis, B. cereus, B. subtilis, B. pumilis, Lactobacillus acidophilus, Proteus mirabilis, Klebsiella pneumoniae, Salmonella choleraesuis, and Salmonella poona. T. serpyllum extract possesses antifungal activity against Fusarium solani, F. moniliforme, Aspergillus flavus, Microsporum canis, Alternaria species, Candida albicans, and C. glabarata. The essential oil efficiently inhibited the growth of C. albicans, C. glabrata and Aspergillus fungi. Depending on the concentration, wild thyme extract significantly relaxed spontaneous contractions, as well as acetylcholine-, potassium chloride-, barium chlorideand calcium chloride-induced contractions of the isolated rat ileum. The extract significantly reduced the expression of the inducible enzyme cyclooxygenase-2 and inhibited acetylcholinesterase, myeloperoxidase and α-glucosidase activity. T. serpyllum extract has shown cytotoxic activity on human breast cancer cell lines, while essential oil has shown the antitumor potential in human cell lung cancer, colon, cervical, hepatocellular, prostate, and breast adeno-carcinoma.",
publisher = "University of East Sarajevo, Faculty of Technology",
journal = "Journal of Engineering & Processing Management",
title = "Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review",
pages = "41-32",
number = "2",
volume = "13",
doi = "10.7251/JEPM2102032J"
}

Jovanović, A. A., Balanč, B. D., Petrović, P., Pravilović, R.,& Đorđević, V. B.. (2021). Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology., 13(2), 32-41.
https://doi.org/10.7251/JEPM2102032J

Jovanović AA, Balanč BD, Petrović P, Pravilović R, Đorđević VB. Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review. in Journal of Engineering & Processing Management. 2021;13(2):32-41.
doi:10.7251/JEPM2102032J .

Jovanović, Aleksandra A., Balanč, Bojana D., Petrović, Predrag, Pravilović, Radoslava, Đorđević, Verica B., "Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review" in Journal of Engineering & Processing Management, 13, no. 2 (2021):32-41,
https://doi.org/10.7251/JEPM2102032J . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6409
AB  - Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries.
AB  - Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.
PB  - Serbian Nutrition Society, Belgrade
T2  - Hrana i ishrana
T1  - Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques
EP  - 20
IS  - 2
SP  - 15
VL  - 62
DO  - 10.5937/hraIsh2102015J
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries., Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.",
publisher = "Serbian Nutrition Society, Belgrade",
journal = "Hrana i ishrana",
title = "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques",
pages = "20-15",
number = "2",
volume = "62",
doi = "10.5937/hraIsh2102015J"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana
Serbian Nutrition Society, Belgrade., 62(2), 15-20.
https://doi.org/10.5937/hraIsh2102015J

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Bugarski B. Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana. 2021;62(2):15-20.
doi:10.5937/hraIsh2102015J .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques" in Hrana i ishrana, 62, no. 2 (2021):15-20,
https://doi.org/10.5937/hraIsh2102015J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6441
AB  - The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.
PB  - Serbian Nutrition Society
C3  - 14th Congress of Nutrition
T1  - The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6441
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko",
year = "2021",
abstract = "The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.",
publisher = "Serbian Nutrition Society",
journal = "14th Congress of Nutrition",
title = "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6441"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K., Nedović, V.,& Bugarski, B.. (2021). The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition
Serbian Nutrition Society..
https://hdl.handle.net/21.15107/rcub_technorep_6441

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Nedović V, Bugarski B. The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition. 2021;:null-76.
https://hdl.handle.net/21.15107/rcub_technorep_6441 .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts" in 14th Congress of Nutrition (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6441 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6410
AB  - Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Thymus serpyllum extract loaded liposomes produced by proliposome method
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6410
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Petrović, Predrag and Đorđević, Verica and Šavikin, Katarina and Zdunić, Gordana and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Thymus serpyllum extract loaded liposomes produced by proliposome method",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6410"
}

Jovanović, A. A., Balanč, B., Petrović, P., Đorđević, V., Šavikin, K., Zdunić, G.,& Bugarski, B.. (2021). Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference
University of Belgrade..
https://hdl.handle.net/21.15107/rcub_technorep_6410

Jovanović AA, Balanč B, Petrović P, Đorđević V, Šavikin K, Zdunić G, Bugarski B. Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference. 2021;:null-144.
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

Jovanović, Aleksandra A., Balanč, Bojana, Petrović, Predrag, Đorđević, Verica, Šavikin, Katarina, Zdunić, Gordana, Bugarski, Branko, "Thymus serpyllum extract loaded liposomes produced by proliposome method" in 2nd International UNIfood Conference (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Mijin, Dušan
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6443
AB  - Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.
PB  - Faculty of Technology, Zvornik
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
T1  - The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme
EP  - 143
SP  - 137
DO  - 10.7251/EEMEN2101137J
ER  - 

@conference{
author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Mijin, Dušan and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.",
publisher = "Faculty of Technology, Zvornik",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM",
title = "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme",
pages = "143-137",
doi = "10.7251/EEMEN2101137J"
}

Jovanović, A., Petrović, P., Đorđević, V., Mijin, D., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
Faculty of Technology, Zvornik., 137-143.
https://doi.org/10.7251/EEMEN2101137J

Jovanović A, Petrović P, Đorđević V, Mijin D, Zdunić G, Šavikin K, Bugarski B. The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM. 2021;:137-143.
doi:10.7251/EEMEN2101137J .

Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Mijin, Dušan, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme" in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM (2021):137-143,
https://doi.org/10.7251/EEMEN2101137J . .

TY  - JOUR
AU  - Drmanić, Saša
AU  - Petrović, Predrag
AU  - Brkić, Dominik R.
AU  - Marinković, Aleksandar
AU  - Nikolić, Jasmina
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4366
AB  - The derivatives of isatin have already been known to display a variety of biological activities. Therefore, the studies on their activity and its relation to structure have recently become a popular subject for investigation. The examined compounds were synthesized by the reaction of isatin and substituted primary amines and characterized by spectroscopic methods. The investigation of the antimicrobial and antioxidative activity of the synthesized compounds was performed by broth microdilution method. As for the characterization of the investigated isatin based Schiff bases, the linear solvation energy relationships (LSER) were used to analyze the solvent influence on the UV absorption maxima shifts (V-max), using the well known Kamlet-Taft model and taking geometrical isomers into consideration when possible. Linear free energy relationships (LFER) were used to analyze substituent effect on pK(a), as well as NMR chemical shifts and v max values. The antimicrobial activity and characterization were related using both experimental and theoretical methods.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - A survey on the characterization and biological activity of isatin derivatives
EP  - 1000
IS  - 8
SP  - 979
VL  - 85
DO  - 10.2298/JSC200320020D
ER  - 

@article{
author = "Drmanić, Saša and Petrović, Predrag and Brkić, Dominik R. and Marinković, Aleksandar and Nikolić, Jasmina",
year = "2020",
abstract = "The derivatives of isatin have already been known to display a variety of biological activities. Therefore, the studies on their activity and its relation to structure have recently become a popular subject for investigation. The examined compounds were synthesized by the reaction of isatin and substituted primary amines and characterized by spectroscopic methods. The investigation of the antimicrobial and antioxidative activity of the synthesized compounds was performed by broth microdilution method. As for the characterization of the investigated isatin based Schiff bases, the linear solvation energy relationships (LSER) were used to analyze the solvent influence on the UV absorption maxima shifts (V-max), using the well known Kamlet-Taft model and taking geometrical isomers into consideration when possible. Linear free energy relationships (LFER) were used to analyze substituent effect on pK(a), as well as NMR chemical shifts and v max values. The antimicrobial activity and characterization were related using both experimental and theoretical methods.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "A survey on the characterization and biological activity of isatin derivatives",
pages = "1000-979",
number = "8",
volume = "85",
doi = "10.2298/JSC200320020D"
}

Drmanić, S., Petrović, P., Brkić, D. R., Marinković, A.,& Nikolić, J.. (2020). A survey on the characterization and biological activity of isatin derivatives. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(8), 979-1000.
https://doi.org/10.2298/JSC200320020D

Drmanić S, Petrović P, Brkić DR, Marinković A, Nikolić J. A survey on the characterization and biological activity of isatin derivatives. in Journal of the Serbian Chemical Society. 2020;85(8):979-1000.
doi:10.2298/JSC200320020D .

Drmanić, Saša, Petrović, Predrag, Brkić, Dominik R., Marinković, Aleksandar, Nikolić, Jasmina, "A survey on the characterization and biological activity of isatin derivatives" in Journal of the Serbian Chemical Society, 85, no. 8 (2020):979-1000,
https://doi.org/10.2298/JSC200320020D . .

TY  - JOUR
AU  - Milošević, Milena D.
AU  - Marinković, Aleksandar
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Nikolić, Milica G.
AU  - Prlainović, Nevena
AU  - Cvijetić, Ilija
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4393
AB  - In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. H-1 and C-13 NMR spectra couldn't be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on.B97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50 = 20 +/- 4 mu M), while molecular docking suggested binding at the peripheral anionic site of AChE with the MMFF94 binding enthalpy of -43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Bioorganic Chemistry
T1  - Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents
VL  - 102
DO  - 10.1016/j.bioorg.2020.104073
ER  - 

@article{
author = "Milošević, Milena D. and Marinković, Aleksandar and Petrović, Predrag and Klaus, Anita and Nikolić, Milica G. and Prlainović, Nevena and Cvijetić, Ilija",
year = "2020",
abstract = "In this study we synthesized a series of sixteen bis(imino)pyridines (BIPs) starting from 2,6-diaminopyridine and various aromatic aldehydes, and evaluated their antioxidant, antibacterial, antifungal and acetylcholinesterase (AChE) inhibitory activity. The chemical structures were elucidated by FTIR, elemental analysis, ESR and HRMS. H-1 and C-13 NMR spectra couldn't be acquired due to the formation of stable, carbon-centered radical cations in a solution, as confirmed by ESR spectroscopy and DFT calculations. The in vitro antioxidant potency was evaluated using four assays: free radical scavenging activity (DPPH and ABTS), reducing power and total antioxidant capacity assay. BIPs demonstrated excellent antioxidant properties, and two derivatives proved to be more potent than reference antioxidants (ascorbic acid and Trolox) in all assays. DFT calculations on.B97XD/6-311++g(d,p) level of theory provided valuable insights into the radical scavenging mechanism of BIPs. For hydroxyl-substituted BIPs, hydrogen atom transfer (HAT) is a predominant mechanism, while the single electron transfer coupled with proton transfer (SET-PT) governs the antioxidant activity of other derivatives. Intramolecular hydrogen bonding (IHB) plays an important role in the mechanism of antioxidant activity as revealed by noncovalent interaction analysis and rotational barrier calculations. The spin density of radical cations is localized on carbon atoms of a pyridine ring, which corroborates with g-factors and multiplicity obtained from ESR analysis. The most potent BIP exhibited moderate inhibitory activity toward AChE (IC50 = 20 +/- 4 mu M), while molecular docking suggested binding at the peripheral anionic site of AChE with the MMFF94 binding enthalpy of -43.4 kcal/mol. Moderate in vitro antimicrobial activity of BIPs have been determined against several pathogenic bacterial strains: Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecalis, Staphylococcus aureus and clinical isolate of methicillin resistant S. aureus (MRSA). The antifungal activity of BIPs toward Candida albicans was also confirmed. The similarity ensemble approach combined with molecular docking suggested leucyl aminopeptidase as the probable antimicrobial target for the three most potent BIP derivatives.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Bioorganic Chemistry",
title = "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents",
volume = "102",
doi = "10.1016/j.bioorg.2020.104073"
}

Milošević, M. D., Marinković, A., Petrović, P., Klaus, A., Nikolić, M. G., Prlainović, N.,& Cvijetić, I.. (2020). Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry
Academic Press Inc Elsevier Science, San Diego., 102.
https://doi.org/10.1016/j.bioorg.2020.104073

Milošević MD, Marinković A, Petrović P, Klaus A, Nikolić MG, Prlainović N, Cvijetić I. Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents. in Bioorganic Chemistry. 2020;102.
doi:10.1016/j.bioorg.2020.104073 .

Milošević, Milena D., Marinković, Aleksandar, Petrović, Predrag, Klaus, Anita, Nikolić, Milica G., Prlainović, Nevena, Cvijetić, Ilija, "Synthesis, characterization and SAR studies of bis(imino)pyridines as antioxidants, acetylcholinesterase inhibitors and antimicrobial agents" in Bioorganic Chemistry, 102 (2020),
https://doi.org/10.1016/j.bioorg.2020.104073 . .